A composition for improving chicken feed conversion efficiency and its application

Abstract

This invention discloses a composition for improving chicken feed conversion efficiency and its application. The composition comprises *Romebutzella roximatei* post-biotic, farnesol, and paeonol in a mass ratio of 2:1:1. The composition is directly added to the feed at a dosage of 1% of the total weight of the chicken feed. This invention finds that the composition formed by the mixture of *Romebutzella roximatei* post-biotic, farnesol, and paeonol, when used as a feed additive, exhibits high safety, no toxic side effects, and can significantly improve broiler feed conversion efficiency, broiler growth performance and meat quality, and laying hen egg production performance and egg quality.

Description

Technical Field

[0001] This invention relates to the field of healthy and efficient chicken farming technology, specifically to a composition for improving chicken feed conversion efficiency and its application. Background Technology

[0002] my country ranks among the world's top producers of poultry meat and eggs. Poultry meat accounts for the second largest share of meat consumption in China, after pork, while eggs are a major source of protein in daily consumption. Therefore, ensuring chicken health, efficient breeding, and strict control over poultry meat and egg production and quality are crucial to national welfare. However, due to various stressors during the breeding process, such as abnormal temperature changes, pathogen infection, transportation stress, excessive vaccination, and harmful factors in feed, chicken production performance and meat / egg quality are negatively impacted; this severely restricts the economic benefits for farmers and chicken production enterprises.

[0003] Therefore, developing green, safe, and efficient feed additive products to improve chicken production performance and meat / egg quality is one of the key issues of concern in the livestock industry. Summary of the Invention

[0004] In view of this, the purpose of this invention is to provide a composition for improving chicken feed conversion efficiency and its application. The composition, by weight, comprises 2 parts of *Rhomboidia rumen-butzion* postbiotic, 1 part of farnesol, and 1 part of paeonol. This composition exhibits high safety, stable efficacy, no toxic side effects, and can significantly improve chicken feed conversion efficiency and meat / egg quality. This composition can be used to prepare chicken feed additives that improve chicken feed conversion efficiency.

[0005] To achieve the above objectives, the present invention provides at least the following technical solutions:

[0006] In a first aspect, the present invention provides a composition for improving chicken feed conversion efficiency, the composition comprising Rombutz bacteria post-biotic, farnesol, and paeonol.

[0007] Secondly, the present invention provides the application of the aforementioned composition in the preparation of chicken feed additives.

[0008] The present invention provides a composition for improving chicken feed conversion efficiency and its application, which has at least the following advantages compared with the prior art:

[0009] 1. The post-biotic of *Romebutzim* overcomes the drawbacks of difficulty in colonizing live bacteria and easy degradation by gastric acid and bile salts, thereby targeting the intestines to improve intestinal health. Farnesol and paeonol are derived from natural plants, exhibiting high safety and no toxic side effects. The combination of *Romebutzim* post-biotic, farnesol, and paeonol can be used as a feed additive for long-term use to improve feed conversion efficiency; long-term use will not lead to the development of drug-resistant strains, an advantage not possessed by other anti-disease drugs.

[0010] 2. In vivo experiments showed that the combination of Rombutz bacteria post-biotic, farnesol and paeonol can improve feed conversion efficiency and meat quality in broilers; and improve egg production performance and egg quality in laying hens. Detailed Implementation

[0011] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the invention.

[0012] Those skilled in the art will understand that, unless otherwise stated, the terms "the," "the," and "the foregoing" used in this application may also include plural forms. It should be further understood that the word "comprising" as used in the specification of this application means the presence of the stated features, steps, or operations, but does not exclude the presence or addition of one or more other features, integers, or steps.

[0013] Those skilled in the art will understand that, where specific experimental steps or conditions are not specified in the embodiments, they can be performed according to the conventional experimental steps or conditions described in the literature in this field; and where the manufacturers of the raw materials or instruments and equipment used are not specified, they are all conventional products that can be obtained commercially.

[0014] Those skilled in the art will understand that, unless otherwise stated in this application, when numerical ranges are given in the embodiments, both endpoints of each numerical range and any value between the two endpoints may be selected. Unless otherwise defined, all technical and scientific terms used in this application, as well as the prior art known to those skilled in the art and the descriptions in this application, can be implemented using any prior art methods, devices, and materials similar to or equivalent to those described, used, or made by means of methods, devices, and materials in the embodiments of this application.

[0015] Romboutsia (RB) primarily colonizes the intestinal lumen and mucosa of the host's ileum. Its metabolism produces various bioactive substances, including β-glucan, functional amino acids, vitamins, propionic acid, and butyric acid. In recent years, due to its high correlation with overall health, RB has attracted widespread attention in the field of biomedicine. For example, studies have found a significant decrease in the relative abundance of RB in the intestinal lumen of patients with autism, hypertension, and diabetes. In the intestines of adult broilers, RB abundance is second only to lactic acid bacteria, indicating its significant potential in regulating broiler gut health and improving growth performance. RB-based postbiotics are rich in β-glucan, functional amino acids, vitamins, propionic acid, and butyric acid, and also contain high levels of bacterial proteins. Compared to live bacteria alone, RB postbiotics overcome the drawbacks of difficult colonization and easy degradation by gastric acid and bile salts, targeting the gut to improve gut health. However, there are currently no reports on RB postbiotics in poultry production.

[0016] Farnesol is an active ingredient extracted from the bark of the acacia tree, primarily composed of flavonoids and triterpenoids. Farnesol is also found in lemongrass, citronella, tuberose, cyclamen, rose, orange blossom, balsam, and musk. Its chemical formula is Ca. 15 H 25 With a molecular weight of 223.72 g / mol, farnesol has been found to possess various biological functions, including antibacterial, anti-inflammatory, antioxidant, and immunomodulatory effects, as well as regulation of lipid metabolism. Clinically, farnesol is used to treat allergic asthma, diabetes, atherosclerosis, obesity, and hyperlipidemia. Due to its green and safe characteristics, farnesol is widely used in food, skincare products, and pharmaceuticals. However, its application in livestock and poultry production is rarely reported.

[0017] Paeonol is a traditional Chinese medicine extracted from the root bark of peony, or the dried root bark of the peony plant (Paeonia suffruticosa). Paeonol is a white or slightly yellow needle-like crystal with a melting point of approximately 49–51°C. It is readily soluble in ethanol and methanol, and slightly soluble in water. Paeonol can be distilled with steam and exhibits strong absorption in the ultraviolet region; its energy at 274 nm is 862, allowing for spectrophotometric determination. Current research has found that paeonol possesses analgesic, anti-inflammatory, antipyretic, and anti-allergic effects. Specifically, paeonol can alleviate pain induced by substances such as acetic acid and acetic acid; it can alleviate inflammatory reactions caused by egg white, formaldehyde, histamine, serotonin, bradykinin, and endotoxins; and it can alleviate fever induced by vaccine immunization. Furthermore, paeonol also possesses various biological functions, including antibacterial, anti-inflammatory, antioxidant, and blood pressure and lipid-regulating effects. Due to its green, safe, and efficient characteristics, paeonol is used as a clinical drug in human medicine, such as paeonol ointment, but there are no reports on paeonol in livestock production.

[0018] Currently, there are few reports on the application of *Rhombus bacillus* metatrophic factors, farnesol, and paeonol in chicken production, and even fewer reports on their application as a combination in chicken production. Based on this, in the embodiments of this invention, *Rhombus bacillus* metatrophic factors, farnesol, and paeonol are mixed in a certain proportion to form a composition. In vivo tests show that this composition can improve feed conversion efficiency and meat quality in broilers; it can also improve egg production performance and egg quality in laying hens, providing basic data for efficient chicken farming.

[0019] Based on this, embodiments of the present invention provide a composition for improving chicken feed conversion efficiency, the composition comprising Rombutz bacteria post-biotic, farnesol, and paeonol.

[0020] In some preferred embodiments, the composition, by weight, comprises 2 parts of Rombutzium postbiotic, 1 part of farnesol, and 1 part of paeonol.

[0021] In some embodiments, the strain of Romboutsia ilealis used to prepare the Romboutsia metagener is commercially available Romboutsia ilealis DSM 25109.

[0022] In some embodiments, the *Rombutz* postbiotic contains ≥33% bacterial cell protein and ≥10% *Rombutz*. 8 cfu / g, β-glucan content ≥5mg / g, sodium butyrate content ≥10mg / g.

[0023] In some preferred embodiments, the preparation method of the Rombutz bacteria postbiotic is as follows:

[0024] A single colony of Romboutsia ilealis DSM 25109 was inoculated into 10 mL of modified GAM liquid medium and anaerobically cultured at 37°C for 24 hours. Then, 1 mL of the bacterial culture was inoculated into 100 mL of modified GAM liquid medium. Next, 100 mL of the bacterial culture was placed in a 500 L fermenter for expansion culture for 24 hours, maintaining the fermenter temperature at a constant 37°C and introducing N2 to establish anaerobic fermentation conditions. After the culture was completed, 10% maltodextrin was added to the fermentation broth for adsorption, and the culture was spray-dried to prepare Romboutsia postbiotics.

[0025] Based on this, embodiments of the present invention provide the application of the aforementioned composition in the preparation of chicken feed additives.

[0026] In some embodiments, the feed additive is used by adding the composition directly to the feed at a rate of 1% of the total weight of the chicken feed.

[0027] The technical solution of the present invention and the technical effects achieved will be described in detail below through more specific embodiments.

[0028] Example 1

[0029] This embodiment provides a composition for improving chicken feed conversion efficiency, which is prepared by the following method:

[0030] Weigh out 2000g of Rombutz bacteria postbiotic (bacterial protein ≥33%, Rombutz bacteria ≥10⁸ CFU / g, β-glucan content ≥5 mg / g, sodium butyrate content ≥10 mg / g), 1000g of farnesol (purchased from Aladdin Company, purity >80%), and 1000g of paeonol (purchased from Aladdin Company, purity >85%), mix them evenly, and you will get the composition for improving chicken feed conversion efficiency.

[0031] The composition can be used in the normal feeding of broilers or laying hens. It is used by adding it to the feed at a rate of 1% of the total feed weight, or adding it to the drinking water at a rate of 1% of the drinking water weight, and using it throughout the feeding cycle.

[0032] Example 2

[0033] This embodiment verifies the effect of the composition prepared in Example 1 on broiler feed conversion efficiency and meat quality. The verification method is as follows:

[0034] 1. Materials and Methods

[0035] The experiment used 240 healthy AA (Animal Humidity) 1-day-old birds with uniform weight. + Male broiler chicks were evenly divided into two treatment groups, each with eight replicates, and each replicate group had 15 chicks. The two treatment groups were a control group and a combination group. The control group broilers were fed a corn-soybean meal basal diet formulated according to the Chinese broiler nutritional requirements standard (NY / T33-2004). The combination group broilers were fed a diet consisting of the combination prepared in Example 1, added at 1% of the total feed weight to the basal diet. The feeding trial lasted for 35 days. Broilers were allowed free access to feed and water and were kept under a 24-hour light regime. On day 35 of the experiment, all broilers were fasted for 8 hours, then weighed and their feed consumption was recorded. Growth performance-related indicators such as average daily gain (ADG), average daily feed intake (ADFI), and feed conversion ratio (FCR) were calculated. Two broilers of uniform weight were selected from each replicate group and anesthetized by intravenous injection of sodium pentobarbital (50 mg / kg live body weight) under the wing vein. They were then slaughtered and their pectoral and leg muscles were dissected for further analysis.

[0036] 2. Data Analysis

[0037] The experimental data were analyzed using one-way ANOVA in SPSS 23.0 statistical software. The significance of the difference was P<0.05 and the extreme significance of the difference was P<0.01. The results are expressed as mean ± standard deviation.

[0038] 3. Test Results

[0039] 3.1 Effects of the composition on broiler growth performance

[0040] Table 1 shows the effect of the composition on the growth performance of broilers. Different letters under the data in the table indicate significant differences (P<0.05). The survival rate is expressed as the number of broilers slaughtered at 35 days of age divided by the number at the start of 1 day of age.

[0041] Table 1

[0042] Testing items control group Composition group P-value d35 - Average body weight, g <![CDATA[2017.63±52.18 b ]]> <![CDATA[2209.12±82.34 a ]]> 0.019 ADFI,g 86.73±4.15 90.26±3.05 0.062 ADG,g <![CDATA[59.32±1.99 b ]]> <![CDATA[64.91±2.73 a ]]> 0.031 FCR <![CDATA[1.47±0.03 a ]]> <![CDATA[1.39±0.02 b ]]> <0.001 Survival rate, % 94.33±1.98 96.14±2.05 0.052

[0043] Table 1 shows that the combination of biotics, farnesol, and paeonol added to the diet significantly increased the average body weight of broilers at 35 days of age, the average daily weight gain during the experiment, and reduced the feed conversion ratio, while also showing a trend towards improving broiler survival rate (P = 0.052). The experimental results indicate that the composition provided by this invention improves broiler feed conversion efficiency and increases broiler survival rate by approximately 1.81%, which has significant application value for farmers and enterprises.

[0044] 3.2 Effect of the composition on the quality of broiler chicken

[0045] Table 2 shows the effects of the composition on broiler meat quality. Different letters under the data indicate significant differences (P < 0.05). Muscle percentage is the ratio of muscle weight to the corresponding broiler body weight × 100%. pH value was measured using a pH meter. Shear force was tested using a computer-controlled muscle tenderness tester (C-LM4, Beijing Tianxiang Feiyu Technology Co., Ltd.). The specific steps are briefly described as follows: A 2cm × 2cm × 2cm meat sample was peeled from the whole chicken breast, placed in a sealed bag, and then placed in an 80℃ constant temperature water bath. After heating for 9.5 minutes with the lid on, the sample was removed and cooled to room temperature for 30 minutes. The liquid inside the bag was then drained, and the meat sample was weighed. The ratio of the muscle sample weight before the test minus the current weight to the muscle weight before the test × 100% represents the muscle cooking loss rate. Further, the square pieces of meat were placed horizontally along the muscle fiber direction at the tenderness tester's blade position and cut twice per piece. The average of the two shear forces was calculated, and the unit is Newtons (N).

[0046] Table 2

[0047]

[0048] Table 2 shows that the combination of *Rhomboidia rumen-derived* post-biotic, farnesol, and paeonol in the diet significantly improved the leg muscle percentage of broilers, showed a trend towards reducing the pH value of the breast muscle (P = 0.063), significantly reduced cooking losses in both breast and leg muscles, decreased shear force in leg muscles, and showed a trend towards reducing shear force in breast muscles (P = 0.074). Lower pH values ​​indicate more acidic muscle, which affects palatability; greater cooking losses mean less usable meat for consumers; lower shear force values ​​indicate higher tenderness. The results in Table 2 indicate that the composition provided in the embodiments of this invention improves muscle deposition efficiency and increases muscle tenderness, thereby improving meat quality.

[0049] Example 3

[0050] This embodiment verifies the effect of the composition prepared in Example 1 on feed conversion efficiency and blood biochemical indicators in broilers under a low-energy model. The verification method is as follows:

[0051] 1. Materials and Methods

[0052] In this experiment, 360 healthy, one-day-old male "Kobo 500" broiler chicks with uniform weight were randomly divided into three treatment groups. Each treatment group included eight replicates, with each replicate containing 15 chicks. The three treatment groups were: a control group, a low-energy group, and a low-energy + combination diet group. The control group was fed a corn-soybean meal basal diet formulated according to the Chinese broiler nutritional requirements standard (NY / T33-2004). The low-energy group was fed a diet that reduced the energy content of the basal diet by 60 kcal / ton of feed by reducing soybean oil. The low-energy + combination diet group was fed a diet that reduced the energy content of the basal diet by 60 kcal / ton of feed. The diets for the broiler group were based on the diets of the low-energy broiler group, with the composition prepared in Example 1 added at a rate of 1% of the total feed weight. The feeding trial lasted for 35 days, during which the broilers had free access to feed and water and were under a 24-hour light regime. On the 35th day of the trial, all broilers were fasted for 8 hours, then weighed and their feed consumption was recorded. Growth performance-related indicators such as average daily gain (ADG), average daily feed intake (ADFI), and feed conversion ratio (FCR) were calculated. Blood biochemical indicators were measured from two broilers of uniform weight in each replicate group by collecting blood from the venous vein under the wing.

[0053] 2. Data Analysis

[0054] The experimental data were analyzed by one-way ANOVA in SPSS 23.0 statistical software. Duncan's method was used to perform multiple comparisons between groups. P < 0.05 was used as the significance criterion, and P < 0.01 was used as the extremely significant criterion. The results are expressed as mean ± standard deviation.

[0055] 3. Test Results

[0056] 3.1 Effects of the composition on broiler growth performance under a low-energy model

[0057] Table 3 shows the effect of the composition on the growth performance of broilers under the low-energy model. Different letters under the data in the table indicate significant differences (P<0.05).

[0058] Table 3

[0059]

[0060]

[0061] Lowering the energy content of poultry diets leads to decreased feed conversion efficiency. Table 3 shows that under the low-energy model, broiler feed conversion efficiency decreases (manifested as an increased feed-to-weight ratio), and the average daily weight gain of broilers decreases. However, adding the composition prepared in this invention to the low-energy model significantly improves both average daily weight gain and broiler feed conversion efficiency.

[0062] 3.2 Effects of the composition on blood biochemical parameters of broilers under a low-energy model

[0063] Table 4 shows the effects of the composition on the blood biochemical parameters of broilers under the low-energy model. Different letters under the data in the table indicate significant differences (P < 0.05). The blood biochemical parameters were measured using a Hitachi fully automated blood biochemical analyzer. TP represents total protein, ALB represents albumin, TB represents total bilirubin, DB represents direct bilirubin, AST represents aspartate aminotransferase, ALT represents alanine aminotransferase, ALP represents alkaline phosphatase, GGT represents gamma-aminotransferase, TG represents triglycerides, TC represents total cholesterol, HDL represents high-density lipoprotein, LDL represents low-density lipoprotein, CREA represents creatinine, UA represents uric acid, GLU represents glucose, and LDH represents lactate dehydrogenase.

[0064] Table 4

[0065] Testing items control group Low energy group Low energy + combination group p-value TP (g / L) 33.67±3.17 35.64±4.00 35.35±2.47 0.379 ALB (g / L) 15.25±0.98 15.74±1.18 15.50±0.87 0.730 TB (g / L) <![CDATA[14.16±2.20 b ]]> <![CDATA[17.87±2.61 a ]]> <![CDATA[14.21±1.07 b ]]> 0.016 DB(μmol / L) <![CDATA[1.29±0.36 b ]]> <![CDATA[1.79±0.42 a ]]> <![CDATA[1.14±0.49 b ]]> <0.001 ALT(U / L) 1.29±0.36 1.79±0.42 1.84±0.49 0.941 AST(U / L) 240.91±34.45 296.75±54.50 251.45±72.78 0.061 ALP(U / L) <![CDATA[2086.68±522.27 b ]]> <![CDATA[2940.05±711.54 a ]]> <![CDATA[1458.26±325.99 c ]]> <0.001 GGT(U / L) 16.50±3.78 18.08±3.92 17.58±3.23 0.791 TG (mmol / L) 0.36±0.07 0.35±0.05 0.37±0.03 0.744 TC (mmol / L) 3.37±0.32 3.54±0.43 3.47±0.32 0.555 HDL (mmol / L) 2.25±0.28 2.41±0.30 2.32±0.22 0.548 LDL (mmol / L) 0.51±0.14 0.51±0.15 0.54±0.12 0.819 CREA (μmol / L) 8.95±2.38 8.87±2.63 8.71±1.72 0.881 UA (μmol / L) 225.20±63.32 255.24±64.49 258.70±62.39 0.254 GLU (mmol / L) 13.44±1.35 13.10±0.59 13.58±1.30 0.733 LDH(U / L) 945.89±728.46 930.61±263.82 976.49±279.91 0.570

[0066] Table 4 shows that under the low-energy model, the levels of total bilirubin, direct bilirubin, alkaline phosphatase, and aspartate aminotransferase in broiler serum increased, indicating that low energy has a negative impact on broiler liver. Under the low-energy model conditions, adding the composition prepared in this invention to the diet can alleviate the increase in the levels of total bilirubin, direct bilirubin, alkaline phosphatase, and aspartate aminotransferase, indicating that the composition has a protective effect on broiler liver. The liver is the main site of nutrient metabolism and an important immune organ. The protective effect of this composition on broiler liver is one of the reasons why it improves feed conversion efficiency in broilers.

[0067] Example 4

[0068] This embodiment verifies the effect of the composition on the egg production performance and egg quality of laying hens. The verification method is as follows:

[0069] 1. Materials and Methods

[0070] In this experiment, 480 healthy 21-week-old Jingfen No. 1 laying hens with uniform weight were randomly divided into two treatment groups: a control group and a combination diet group. Each treatment group had 8 replicates, with 30 laying hens in each replicate group. The control group was fed a corn-soybean meal basal diet formulated according to the Chinese Nutritional Requirements Standard for Laying Hens (NY / T33-2004). The combination diet group was fed a diet consisting of the combination prepared in Example 1, added at 1% of the total feed weight to the basal diet. The experiment lasted for 5 weeks. During the experiment, the laying hens had free access to feed and water, and an 18-hour light-6-hour dark lighting regime was used. Egg weight was recorded daily, and egg production rate and feed consumption were recorded weekly to calculate the feed conversion ratio. At the end of the experiment, 60 eggs were randomly selected from each treatment group for egg quality analysis.

[0071] 2. Data Analysis

[0072] The experimental data were analyzed using one-way ANOVA in SPSS 23.0 statistical software. The significance of the difference was P<0.05 and the extreme significance of the difference was P<0.01. The results are expressed as mean ± standard deviation.

[0073] 3. Test Results

[0074] 3.1 Effect of the composition on egg production rate of laying hens

[0075] Table 5 shows the effect of the composition on the egg production rate of laying hens. Different letters under the data in the table indicate significant differences (P<0.05).

[0076] Table 5

[0077] time control group Composition group p-value Week 1 25.61±2.43 26.12±3.32 0.484 Week 2 51.24±3.65 52.11±3.37 0.628 Week 3 <![CDATA[74.08±5.11 b ]]> <![CDATA[81.42±3.22 a ]]> 0.016 Week 4 <![CDATA[84.32±3.21 b ]]> <![CDATA[90.39±5.15 a ]]> 0.021 Week 5 91.90±2.91 93.47±4.43 0.056

[0078] As shown in Table 5, adding the composition provided by this invention to the diet can significantly improve the egg production rate of laying hens in weeks 3, 4 and 5 of the experiment.

[0079] 3.2 Effect of the composition on the feed conversion ratio of laying hens

[0080] Table 6 shows the effect of the composition on the feed conversion ratio of laying hens; the results are expressed as the feed conversion ratio of laying hens in each week of the experiment and throughout the entire experiment period. The feed conversion ratio represents the weight of feed (in kilograms) required to produce 1 kilogram of eggs.

[0081] Table 6

[0082]

[0083]

[0084] Table 6 shows that the combination of *Rhodomyrtus tomentosa* post-biotic, farnesol, and paeonol in the diet improved the feed conversion ratio (FCR) of laying hens in weeks 3, 4, and 5 of the experiment, and had an improving effect on the FCR throughout the entire experiment. The experimental results indicate that the composition provided by this invention can improve the feed conversion efficiency of laying hens and save on breeding costs.

[0085] 3.3 Effect of the composition on egg quality

[0086] Table 7 shows the effects of the composition on egg quality, where the egg quality testing methods are as follows:

[0087] After weighing the eggs using an analytical balance, the eggshell strength (kg / cm²) was measured using an egg quality tester DET-6000 (NABEL Co., Ltd, Japan). 2 The test involves measuring the egg's yolk color and, specifically, placing the egg vertically on an eggshell strength tester with the blunt end facing upwards, and measuring the pressure exerted per unit area on the eggshell surface. The albumen height (mm) is measured using an albumen height meter (KIYA-818B, SEISAKUSHO, LTD), and the Haugh unit is calculated using the formula: Haugh unit = 100·lg(H - 1.7W). 0.37 +7.57), where H is the albumen height (mm) and W is the egg weight (g); the eggshell thickness is measured using a micrometer (after removing the shell membrane, the thickness of the middle, blunt, and sharp ends of the egg is measured and the average value is taken in millimeters, accurate to 0.01mm).

[0088] Table 7

[0089] Testing items control group Composition group p-value Protein height, mm 6.33±0.95 6.56±0.87 0.845 Haas unit 73.17±8.22 75.35±5.09 0.511 Egg yolk color 6.38±0.77 6.41±0.78 0.798 <![CDATA[Eggshell strength, kg / cm 2 > 40.15±3.15 44.01±2.18 0.029 Eggshell thickness, cm 0.33±0.01 0.36±0.01 0.002 Egg yolk ratio, % 22.33±1.52 23.06±1.67 0.176 Protein percentage, % 66.87±1.44 65.77±0.89 0.221 Eggshell percentage, % 10.75±0.38 10.51±0.99 0.537

[0090] Table 7 shows that the combination of *Rhombus aestivum* post-biotic, farnesol, and paeonol in the diet can improve the eggshell strength and thickness. Higher eggshell strength and thickness result in better storage and transportation resistance. These results indicate that the composition provided by this invention improves egg quality by enhancing eggshell quality.

[0091] In summary, the composition formed by mixing Rombutzella post-biotic, farnesol and paeonol provided by the present invention can improve the conversion efficiency of chicken feed, and this composition can be used to prepare chicken feed additives.

[0092] The present invention has been described in detail above. Specific examples have been used to illustrate the principles and implementation methods of the invention. The descriptions of the embodiments above are only for the purpose of helping to understand the invention and its core ideas. It should be noted that those skilled in the art can make various improvements and modifications to the invention without departing from its principles, and these improvements and modifications also fall within the protection scope of the claims of the present invention.

Claims

1. A composition for improving chicken feed conversion efficiency, said composition comprising Rombutzella post-biotic, farnesol, and paeonol; The composition, by weight, consists of 2 parts of Rombutz bacteria post-biotic, 1 part of farnesol, and 1 part of paeonol; The Rombutz strain used to prepare Rombutz metabiotics is commercially available. Romboutsia ilealis DSM25109.

2. The composition according to claim 1, characterized in that, The *Rombutz* postbiotic contains ≥33% bacterial cell protein and ≥10% *Rombutz*. 8 cfu / g, β-glucan content ≥5 mg / g, sodium butyrate content ≥10 mg / g.

3. The composition according to claim 1, characterized in that, The preparation method of the Rombutz bacteria postbiotic is as follows: Rombutz Romboutsia ilealis A single colony of DSM 25109 was inoculated into 10 mL of modified GAM liquid medium and anaerobically cultured at 37 °C for 24 hours. Subsequently, 1 mL of the bacterial culture was inoculated into 100 mL of modified GAM liquid medium. Then, 100 mL of the bacterial culture was placed in a 500 L fermenter for expansion culture for 24 hours, maintaining the fermenter temperature at a constant 37 °C and introducing N2 to establish anaerobic fermentation conditions. After the culture was completed, 10% maltodextrin was added to the fermentation broth for adsorption, and spray-dried to prepare the post-biotic of Rombutz.

4. The use of the composition according to any one of claims 1 to 3 in the preparation of chicken feed additives.

5. The application according to claim 4, characterized in that, The method of using the feed additive is as follows: add the composition directly to the feed, and the amount added is 1% of the total weight of the chicken feed.

Images