A sampling device and detection method for detecting calf diarrhea pathogens

Abstract

The application discloses a kind of calf diarrhea pathogen detection sampling device, including sampling tube, adjusting pipe, sampling membrane cover and extraction assembly;Sampling tube distal end is fixedly connected with adjusting pipe, and the hollow section in sampling tube includes communicating rectangle and circle;The section of sampling tube proximal end is rectangle hollow, and upper and lower sides are provided with telescopic cavity;Sampling membrane cover is sleeved on the outside of sampling tube;Adjusting pipe is rotatably connected with extraction assembly;Extraction assembly has negative pressure function, so that sampling membrane cover is sucked into sampling tube under the action of negative pressure;A pair of infusion holes are provided in the inside of sampling tube, and the input end of infusion hole penetrates into the inside of adjusting pipe, and penetrates out from the outer wall of adjusting pipe;The output end of infusion hole penetrates into telescopic cavity;It also includes a pair of plugging components and connecting pipes;A pair of plugging components are arranged in telescopic cavity;Each plugging component is fixedly connected with the output end of infusion hole;Connecting pipe is fixedly connected with plugging component at both ends.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of calf diarrhea sampling device, specifically to a sampling device for calf diarrhea pathogen detection and a detection method. BACKGROUND

[0002] CN114129192B discloses a rapid sampling device for calf diarrhea pathogen detection. The specific sampling method is to form a sealed capsule: the sampler rotates the handle to make the inner tube rotate with the handle, the inner tube rotates in the composite tube through the rotating sleeve, and the composite tube is in a state of being pressed by the cow's rectum, so there is resistance between the composite tube and the cow's rectum. When the inner tube rotates, the speed of the inner tube is greater than that of the composite tube, and there is a speed difference. When the inner tube rotates, the twisting resistance of the inner tube to the cylindrical membrane tube is increased by the pressing plate, the cylindrical membrane tube located at the front end of the inner tube is twisted, and the cylindrical membrane tube is twisted into a sealed capsule, so that the sampled feces is stored in the sealed capsule.

[0003] The rectum of the calf is very fragile, and using the resistance of the inner wall of the rectum of the calf to reduce the speed of the composite tube will undoubtedly cause damage to the rectum of the calf, making the calf feel uncomfortable, and the calf will perform various actions, thereby affecting the smooth progress of sampling.

[0004] Since the composite tube and the inner tube can rotate in the rectum, it can be seen that the composite tube and the inner tube are made of hard material. When the cow feels pain due to the rotation of the composite tube, various actions will occur, and the hard end of the composite tube is also likely to cause damage to the rectum of the cow.

[0005] The part of the rectum of the calf close to the anus is naturally curved to adapt to the change of abdominal pressure and defecation action. Therefore, the depth of insertion of the composite tube and the inner tube into the rectum is limited, and it is not possible to ensure that enough fecal samples can be obtained. Therefore, the present application provides a sampling device for calf diarrhea pathogen detection and a detection method. SUMMARY

[0006] The present application aims to provide a sampling device for calf diarrhea pathogen detection to solve the problem of damage to the rectum of the calf during sampling as described in the background.

[0007] To achieve the above-mentioned purpose, the present application provides the following technical solutions:

[0008] A sampling device for calf diarrhea pathogen detection, comprising a sampling tube, an adjusting tube, a sampling membrane sleeve and a extraction assembly; the distal end of the sampling tube is fixedly connected with the adjusting tube, the hollow cross section of the sampling tube comprises a rectangle and a circle which are in communication; the hollow cross section of the sampling tube close to the proximal end is a rectangle, and the hollow cross section of the sampling tube close to the distal end is a circle; the proximal end of the sampling tube is a rectangular hollow, and the upper and lower sides of the rectangular hollow are provided with telescopic cavities;

[0009] The sampling membrane sleeve is sleeved outside the sampling tube; the adjusting tube is rotationally connected with the extraction assembly; the extraction assembly has a negative pressure function, so that the sampling membrane sleeve is sucked into the sampling tube under the action of the negative pressure; a pair of infusion holes are formed in the inside of the sampling tube, the infusion hole input end penetrates into the inside of the adjusting tube, and the infusion hole output end penetrates out of the outer wall of the adjusting tube; the infusion hole output end penetrates into the telescopic cavity;

[0010] Further comprising a pair of sealing assemblies and a connecting tube; the pair of sealing assemblies are arranged in the telescopic cavity; each sealing assembly is fixedly connected with the infusion hole output end; the connecting tube is fixedly connected with the sealing assemblies at both ends; the sealing assemblies have a telescopic function, and when the pair of sealing assemblies are heated to a specified temperature, the sealing assemblies start to stretch towards each other, and vice versa; so as to deflate the sampling membrane sleeve in the rectangular cross-section through hole.

[0011] Preferably, the adjusting tube is fixedly connected with a fixed ring on the outer wall; the rear end of the sampling membrane sleeve is an open section; the open section is detachably connected on the side of the fixed ring away from the extraction assembly; the front end of the sampling membrane sleeve is a closed end, and is located at the proximal end of the sampling tube, and is used for entering the sampling tube with feces.

[0012] Preferably, the sampling tube is a flexible hose with toughness, and the proximal end is arc-shaped; a flexible heat insulation strip is arranged between the opposite side walls of the sealing assembly and the connecting tube, and the heat insulation strip is fixedly connected with the sealing assembly and the connecting tube; a heat insulation layer is pasted on the inner wall of the sampling tube between the heat insulation strip and the adjusting tube; the adjusting tube is a hard tube; the sampling membrane sleeve is made of flexible material; and the connecting tube is a flexible hose.

[0013] Preferably, the extraction assembly comprises a rotating pipe, a piston pipe, a piston and a sealing plate; the rotating pipe is rotationally connected with the adjusting tube and the piston pipe at both ends respectively; the piston is slidingly connected in the piston pipe; the sealing plate is fixedly connected on the inner wall of the piston pipe and is located at the end of the piston pipe close to the rotating pipe; the upper side of the sealing plate is lower than the center of the piston pipe; a pair of symmetrical air holes are formed in the inner wall of the piston pipe on the inner side of the sealing plate.

[0014] Preferably, the rotating pipe comprises a rotating tube, a rotating plate, a cross rod and a pair of sealing plates; the rotating tube is rotationally connected with the adjusting tube and the piston pipe at both ends respectively; the rotating plate is fixedly connected on the inner wall of the rotating tube close to the end of the piston pipe, and the lower side of the rotating plate exceeds the center of the rotating tube and also exceeds the upper side of the sealing plate; the cross rod is fixedly connected on the rotating plate; the cross rod is fixedly connected with a pair of sealing plates at the two vertical ends of the rotating tube; and the sealing plates correspond to the pair of air holes.

[0015] Preferably, the rotating pipe further comprises a positioning block; the positioning block is fixedly connected on the side of the sealing plate close to the rotating plate; and the positioning block is lower than the upper side of the sealing plate.

[0016] Preferably, each plugging assembly comprises an expansion rod, a heating pipe and a pressing plate; a pair of pressing plates are located on the upper and lower sides of the rectangular through hole; each expansion rod is fixedly connected to the corresponding expansion cavity and the pressing plate at both ends; a pair of heating pipes are fixedly connected to the same side of the expansion rod for expansion and contraction with the expansion rod; both ends of the heating pipe are fixedly connected to the connecting pipe and the output end of the infusion hole, respectively; the middle of the connecting pipe is fixedly connected to the side of the rectangular through hole.

[0017] Preferably, the expansion rod is made of memory alloy material; the deformation temperature of the memory alloy material is 50-60℃, preferably 50℃.

[0018] Preferably, a hole cap is inserted into the input end of one of the infusion holes; an injection hose is fixedly connected to the input end of the other infusion hole; a plurality of air holes are also provided on the adjusting pipe, which are communicated to the side wall of the input end of the other infusion hole.

[0019] Also provided is a detection method of the sampling device for detecting the diarrhea pathogen of a calf, comprising the following steps:

[0020] S1, first, the sampling membrane sleeve is sleeved on the sampling tube; the rotating pipe is rotated to block the air hole with the plugging plate; the hole cap is inserted into the input end of the infusion hole on the lower side of the adjusting pipe;

[0021] S2, the proximal end of the sampling tube is inserted into the rectum of the calf;

[0022] S3, during the insertion process, the piston is pulled outward; the sampling membrane sleeve and the feces are sucked into the sampling tube through the negative pressure generated by the piston in the piston tube;

[0023] S4, when the piston moves outward to the outer end of the piston tube, the insertion of the sampling tube into the rectum is stopped; the rotating pipe is reversely rotated to make the plugging plate disengage from the air hole; then the piston is reversely pushed, and the gas in the piston tube is discharged through the air hole; the air hole is blocked again; then the piston is continuously pulled outward, and the process is repeated, so that the sampling membrane sleeve is continuously sucked into the sampling tube to collect more feces.

[0024] S5, after the sampling is completed, hot water at 50-60℃, preferably 50℃, is injected into the injection hose; the hot water flows to the heating pipe through the infusion hole and heats the expansion rod; when the pair of expansion rods are heated to the specified temperature, they start to stretch towards each other, thereby driving the pair of pressing plates to move towards each other to flatten the sampling membrane sleeve located between the pair of pressing plates;

[0025] S6, the sampling tube is pulled out of the rectum of the calf; then the hole cap is removed, and cold water is injected into the injection hose again to cool the expansion rod, so that the expansion rod shrinks back into the expansion cavity; then the sampling membrane sleeve is detached from the fixing ring, and the sampling membrane sleeve in the sampling tube is pulled out; the sampling is completed.

[0026] S7, finally inject gas into the injection hose, the residual liquid in the injection hose, infusion hole, heating tube and connecting pipe is discharged.

[0027] Compared with the prior art, the present application has the following advantages:

[0028] The present application has the advantages of simple structure, convenient operation and safe sampling process. The sampling tube has flexibility and toughness, and can be inserted into the rectum of a cow. When the sampling tube is inserted into the rectum of the cow, it can bend along the bending state of the rectum of the cow, and the arc-shaped proximal end can prevent the sampling tube from causing damage to the rectum of the cow and reducing the pain of the cow. More sampling tubes can be inserted into the rectum of the cow to collect more fecal samples. After the sampling tube is pulled out, the sampling membrane sleeve can be removed from the sampling tube, and the structure of the present application can be reused.

[0029] During the insertion of the sampling tube into the rectum of the cow, the piston member is pulled outward to generate negative pressure, so that the sampling membrane sleeve and the feces are sucked into the sampling tube. Under the action of negative pressure, the feces will not flow out of the sampling tube.

[0030] By using the telescopic rod made of memory alloy material, the sampling membrane sleeve between the pressing plates can be pressed flat after heating and cooperating with the pressing plates to prevent the feces from flowing out during the extraction of the sampling tube. Hot water or cold water is input into the heating tube through the infusion hole to control the temperature of the telescopic rod to make the telescopic rod stretch or contract. Since the infusion hole is arranged inside the sampling tube, the hot water flows between the infusion hole and the heating tube, and the sampling tube is heat-insulated, so that the hot water does not contact the rectum of the calf, avoiding damage to the rectum of the calf. BRIEF DESCRIPTION OF DRAWINGS

[0031] Figure 1 The present application is a schematic diagram of the structure;

[0032] Figure 2 The present application is a schematic diagram of the structure;

[0033] Figure 3 The present application is a schematic diagram of the structure; Figure 2 The present application is a schematic diagram of the structure;

[0034] Figure 4 The present application is a schematic diagram of the structure;

[0035] Figure 5 The present application is a schematic diagram of the structure;

[0036] Figure 6 The present application is a schematic diagram of the structure;

[0037] Figure 7 The present application is a schematic diagram of the structure; Figure 6Structure section view of the blocking assembly at middle B;

[0038] Figure 8 Structure section view of the connection between the blocking assembly and the telescopic cavity of the present application;

[0039] Figure 9 Structure section view of the present application after the telescopic plate is stretched;

[0040] Figure 10 Positioning schematic diagram of the present application inserted into the rectum of a calf;

[0041] Figure 11 Structure section view of the heat insulation layer in the sampling tube of the present application;

[0042] Figure 12 Structure section view of the connection between the connecting tube and the heat insulation strip of the present application.

[0043] In the figure: 11 infusion hole, 12 sampling tube, 121 telescopic cavity, 13 adjusting tube, 131 fixing ring, 21 sampling membrane sleeve, 3 extraction assembly, 31 rotating tube, 311 rotating tube, 312 rotating plate, 313 cross bar, 314 blocking plate, 32 piston tube, 321 air hole, 33 piston, 34 closing plate, 35 positioning block, 4 blocking assembly, 41 telescopic rod, 42 heating tube, 43 extrusion plate, 5 connecting tube, 61 hole cap, 62 injection hose, 63 air hole, 7 heat insulation strip, 8 heat insulation layer. DETAILED DESCRIPTION

[0044] Example 1

[0045] Please refer to Figures 1-10 , the present application provides a technical solution: as Figures 1-3 shown, a sampling device for detecting diarrhea pathogens of a calf, comprising a sampling tube 12, an adjusting tube 13, a sampling membrane sleeve 21 and an extraction assembly 3. The distal end of the sampling tube 12 is fixedly connected with the adjusting tube 13, the sampling tube 12 and the adjusting tube 13 are hollow inside and communicate with each other, the hollow cross section inside the sampling tube 12 includes a communicating rectangle and a circle; the hollow cross section of the sampling tube 12 near the proximal end is rectangular, and the hollow cross section of the sampling tube 12 near the distal end is circular. The proximal end cross section of the sampling tube 12 is rectangular hollow, and the upper and lower sides of the rectangular hollow are provided with telescopic cavities 121.

[0046] The adjusting tube 13 is fixedly connected with a fixing ring 131. The sampling membrane sleeve 21 is sleeved outside the sampling tube 12, the rear end of the sampling membrane sleeve 21 is an open section and is detachably connected on the side of the fixing ring 131 away from the extraction assembly 3; the front end of the sampling membrane sleeve 21 is a closed end and is located at the proximal end of the sampling tube 12, for bringing feces into the sampling tube 12. The adjusting tube 13 is rotationally connected with the extraction assembly 3. The extraction assembly 3 has a negative pressure function, so as to suck the front end of the sampling membrane sleeve 21 into the sampling tube 12 under the action of negative pressure.

[0047] The sampling tube 12 is internally provided with a pair of infusion holes 11, the input end of the infusion hole 11 penetrates into the inside of the adjusting tube 13, and penetrates out from the outer wall of the adjusting tube 13; the output end of the infusion hole 11 penetrates into the telescopic cavity 121.

[0048] The application also comprises a pair of sealing assemblies 4 and a connecting tube 5. The pair of sealing assemblies 4 are arranged in the telescopic cavity 121. Each sealing assembly 4 is fixedly connected with the output end of the infusion hole 11. The connecting tube 5 is fixedly connected with the sealing assemblies 4 at both ends, and is attached to the side of the rectangular through hole. The infusion hole 11 is used for conveying fluid for the sealing assembly 4. The fluid is hot water, and the temperature of the hot water is 50-60 DEG C.

[0049] The sealing assembly 4 has a telescopic function. When the fluid contacts the sealing assembly 4, the pair of sealing assemblies 4 are heated to a specified temperature and then start to stretch towards each other, so as to compress the sampling membrane sleeve 21 located in the rectangular through hole.

[0050] The sampling tube 12 is a soft tube with certain toughness and good heat insulation effect, and the proximal end of the sampling tube 12 is arc-shaped. The adjusting tube 13 is a hard tube. The sampling membrane sleeve 21 is made of flexible material. The connecting tube 5 is a soft tube.

[0051] As shown in Figures 3-5 The extraction assembly 3 comprises a rotating pipe 31, a piston pipe 32, a piston 33 and a sealing plate 34. One end of the rotating pipe 31 is rotatably connected with the adjusting tube 13, and the rotating pipe 31 comprises a rotating pipe 311, a rotating plate 312, a cross rod 313 and a pair of sealing plates 314. Specifically, one end of the rotating pipe 311 is rotatably connected with the adjusting tube 13, and the other end is rotatably connected with the piston pipe 32. The sealing plate 34 is fixedly connected to the inner wall of the piston pipe 32, and the sealing plate 34 is arranged at the end of the piston pipe 32 close to the rotating pipe 31. The upper side of the sealing plate 34 is lower than the center of the piston pipe 32. A pair of symmetrical air holes 321 are arranged on the inner wall of the piston pipe 32 located on the inner side of the sealing plate 34.

[0052] The rotating plate 312 is fixedly connected to the inner wall of the rotating pipe 311 close to the end of the piston pipe 32, and the lower side of the rotating plate 312 exceeds the center of the rotating pipe 311 and also exceeds the upper side of the sealing plate 34. The cross rod 313 is fixedly connected to the rotating plate 312 and coincides with the center of the rotating pipe 311. The cross rod 313 is fixedly connected with a pair of sealing plates 314 at the vertical two ends of the rotating pipe 311. The sealing plates 314 correspond to the pair of air holes 321.

[0053] The rotating pipe 31 further comprises a positioning block 35. The positioning block 35 is fixedly connected to the closing plate 34 near the side of the rotating plate 312. The positioning block 35 is lower than the upper side of the closing plate 34, and is used to position the rotating plate 312. When the lower side of the rotating plate 312 is located on the upper side of the positioning block 35, the rotating plate 312 will close the upper side of the closing plate 34, so that the inside of the rotating pipe 311 and the piston pipe 32 are not communicated. Conversely, when the rotating plate 312 is rotated away from the positioning block 35, the inside of the rotating pipe 311 and the piston pipe 32 begin to communicate; when the blocking plate 314 blocks the air hole 321, the area of the inside of the rotating pipe 311 and the piston pipe 32 that are communicated is the largest, at this time, pulling the piston piece 33 outward will generate a negative pressure, which can suck the sampling membrane sleeve 21 into the sampling pipe 12, and further move in the sampling pipe 12.

[0054] As shown in Figure 2 and Figures 6-10 Each blocking assembly 4 comprises an extension rod 41, a heating pipe 42 and a pressing plate 43. A pair of pressing plates 43 are located on the upper and lower sides of a rectangular through hole. Each extension rod 41 is fixedly connected to the corresponding extension cavity 121 and the pressing plate 43 at both ends. A pair of heating pipes 42 are fixedly connected to the same side of the extension rod 41, and the length of the heating pipe 42 is consistent with the length of the extension rod 41, so as to follow the extension rod 41 to extend and retract. The two ends of the heating pipe 42 are respectively fixedly connected to the connecting pipe 5 and the output end of the infusion hole 11. So that the inside of the pair of infusion holes 11, the pair of heating pipes 42 and the connecting pipe 5 are communicated. The middle part of the connecting pipe 5 is fixedly connected to the side of the rectangular through hole, so as to limit the position of the connecting pipe 5.

[0055] The extension rod 41 is made of memory alloy material. The deformation temperature of the memory alloy material is set to 50 degrees. Below 50 degrees, the extension rod 41 is folded in Z shape in the extension cavity 121. When hot water is input into the heating pipe 42 through the infusion hole 11, the extension rod 41 starts to deform after the temperature rises to 50 degrees, and stretches in the direction of the rectangular through hole; so as to drive the pressing plate 43 to press the sampling membrane sleeve 21; the temperature of the hot water is between 50-60℃, which can also ensure the safety of the operator.

[0056] As shown in Figure 3As shown, the input end of the infusion hole 11 at the lower side of the adjusting pipe 13 is inserted with a hole cap 61, which is fixedly connected to the outer wall of the adjusting pipe 13 by a rope. The input end of the infusion hole 11 at the upper side of the adjusting pipe 13 is fixedly connected with an injection hose 62, which facilitates the injection of hot water into the infusion hole 11 through an injection needle. A plurality of air vents 63 are formed on the adjusting pipe 13 and communicate with the input end of the infusion hole 11 at the upper side. When the input end of the infusion hole 11 at the lower side of the adjusting pipe 13 is inserted with the hole cap 61 and hot water is injected into the input end of the infusion hole 11 at the upper side of the adjusting pipe 13, the air vents 63 are used to discharge the gas in the infusion hole 11, the heating pipe 42 and the connecting pipe 5, so that the heating pipe 42 is filled with hot water, thereby ensuring the heating effect on the telescopic rod 41.

[0057] A detection method of a sampling device for detecting a calf diarrhea pathogen, comprising the following steps:

[0058] S1, first, the sampling membrane sleeve 21 is sleeved on the sampling pipe 12; and the closed end of the sampling membrane sleeve 21 is located at the proximal end of the sampling pipe 12. The rotating pipe 31 is rotated, so that the blocking plate 314 blocks the air vent 321. The hole cap 61 is inserted into the input end of the infusion hole 11 at the lower side of the adjusting pipe 13.

[0059] S2, the proximal end of the sampling pipe 12 is slowly inserted into the rectum of the calf. The sampling pipe 12 has a certain toughness and can change the bending degree according to the shape of the rectum, so as to reduce the burden and damage to the rectum of the calf.

[0060] S3, during the insertion process, the piston piece 33 is pulled outward. The negative pressure generated by the piston piece 33 in the piston pipe 32 sucks the closed end of the sampling membrane sleeve 21 into the sampling pipe 12; as the sampling pipe 12 continues to be inserted, the sampling membrane sleeve 21 continuously enters the sampling pipe 12 with the feces in the rectum.

[0061] S4, when the piston piece 33 moves outward to the outer end of the piston pipe 32, the insertion of the sampling pipe 12 into the rectum is stopped. The rotating pipe 31 is reversely rotated, so that the lower side of the rotating pipe 311 abuts against the positioning block 35, and the hollowed sealing of the sealing rod 34 is closed, at this time, the blocking plate 314 is separated from the air vent 321. Then the piston piece 33 is reversely pushed, and the gas in the piston pipe 32 is discharged through the air vent 321. The rotating pipe 31 is rotated again, so that the blocking plate 314 blocks the air vent 321. Then the piston piece 33 is continuously pulled outward, so as to be repeated, thereby continuously sucking the sampling membrane sleeve 21 into the sampling pipe 12 to collect more feces.

[0062] S5, after the sampling is completed, hot water at 50 degrees is injected into the injection hose 62 through the injection tube (not shown in the figure). The hot water flows through the infusion hole 11 to the heating tube 42 to heat the telescopic rod 41. When the pair of telescopic rods 41 are heated to the specified temperature, they start to stretch towards each other, thereby driving the pair of pressing plates 43 to move towards each other to collapse the sampling membrane sleeve 21 between the pair of pressing plates 43 to prevent the feces from flowing out. The infusion hole 11 is at a certain distance from the inner wall of the sampling tube 12 and has a small volume, so when the hot water flows in the infusion hole 11 and the heating tube 42, the temperature rise of the inner wall of the sampling tube 12 is limited. In this way, the temperature of the sampling tube 12 is prevented from being too high to affect the collected feces.

[0063] S6, the sampling tube 12 is pulled out of the calf rectum. Then the hole cap 61 is removed, and cold water is injected into the injection hose 62 through the injection tube again to push out the hot water and cool the telescopic rod 41, so that the telescopic rod 41 is retracted into the telescopic cavity 121. Then the open end of the sampling membrane sleeve 21 is detached from the fixing ring 131, and the sampling membrane sleeve 21 in the sampling tube 12 is pulled out. The sampling is completed.

[0064] S7, finally, gas is injected into the injection hose 62 through the injection tube to discharge the liquid remaining in the injection hose 62, the infusion hole 11, the heating tube 42 and the connecting tube 5.

[0065] Example 2

[0066] As Figures 11-12 shown, on the basis of example 1, a flexible heat insulation strip 7 is arranged between the sampling tube 12 and the connecting tube 5, and the heat insulation strip 7 is fixedly connected with the pressing plate 43 and the connecting tube 5. When the pressing plate 43 moves, it can move with the connecting tube 5. When the hot water flows from the connecting tube 5, the heat is insulated to prevent heat transfer to the rectangular hollow part and damage the feces sample.

[0067] A heat insulation layer 8 is attached to the inner wall of the sampling tube 12 between the heat insulation strip 7 and the adjusting tube 13.

[0068] The heat insulation layer 8 and the sampling tube 12 together provide double-layer heat insulation to protect the feces in the sampling tube 12. The heat insulation strip 7 and the heat insulation layer 8 are made of heat insulation materials, such as aerogel felt, heat insulation paper or other heat insulation materials.

Claims

1. A sampling device for detecting pathogens causing diarrhea in calves, characterized in that: It includes a sampling tube (12), an adjustment tube (13), a sampling membrane sleeve (21), and an extraction assembly (3); the distal end of the sampling tube (12) is fixedly connected to the adjustment tube (13), and the hollow cross section inside the sampling tube (12) includes a connected rectangle and a circle; the hollow cross section near the proximal end of the sampling tube (12) is rectangular, and the hollow cross section near the distal end is circular; the upper and lower sides of the rectangular hollow cross section of the sampling tube (12) are provided with telescopic cavities (121). The sampling membrane sleeve (21) is fitted over the outside of the sampling tube (12); the regulating tube (13) is rotatably connected to the extraction component (3); the extraction component (3) has a negative pressure function, so that the sampling membrane sleeve (21) is sucked into the sampling tube (12) under the action of negative pressure; a pair of infusion holes (11) are provided inside the sampling tube (12), the inlet end of the infusion hole (11) passes through the inside of the regulating tube (13) and exits from the outer wall of the regulating tube (13); the outlet end of the infusion hole (11) passes through the telescopic cavity (121). It also includes a pair of sealing components (4) and a connecting tube (5); the pair of sealing components (4) are set in the telescopic cavity (121); each sealing component (4) is fixedly connected to the output end of the infusion hole (11); the two ends of the connecting tube (5) are fixedly connected to the sealing components (4); the sealing components (4) have a telescopic function, and when the pair of sealing components (4) are heated to a specified temperature, they begin to stretch towards each other, and conversely, the sealing components (4) shrink; so as to crush the sampling membrane sleeve (21) located in the through hole with a rectangular cross section.

2. The sampling device for detecting pathogens causing calf diarrhea according to claim 1, characterized in that: The outer wall of the regulating tube (13) is fixedly connected to a fixing ring (131); the rear end of the sampling membrane sleeve (21) is an open section; the open section is detachably connected to the side of the fixing ring (131) away from the extraction component (3); the front end of the sampling membrane sleeve (21) is a closed end, located at the far end of the sampling tube (12), used to carry feces into the sampling tube (12).

3. The sampling device for detecting pathogens causing calf diarrhea according to claim 1, characterized in that: The sampling tube (12) is a flexible hose with an arc-shaped proximal end; a flexible heat insulation strip (7) is provided between the opposite sidewalls of the sealing component (4) and the connecting tube (5), and the heat insulation strip (7) is fixedly connected to the sealing component (4) and the connecting tube (5); a heat insulation layer (8) is pasted on the inner wall of the sampling tube (12) located between the heat insulation strip (7) and the regulating tube (13); the regulating tube (13) is a rigid tube; the sampling membrane sleeve (21) is made of flexible material; and the connecting tube (5) is a flexible hose.

4. The sampling device for detecting pathogens causing calf diarrhea according to claim 1, characterized in that: The extraction assembly (3) includes a rotating tube (31), a piston tube (32), a piston (33), and a sealing plate (34); the two ends of the rotating tube (31) are rotatably connected to the adjusting tube (13) and the piston tube (32), respectively; the piston (33) is slidably connected inside the piston tube (32); the sealing plate (34) is fixedly connected to the inner wall of the piston tube (32) and is located at the end of the piston tube (32) near the rotating tube (31); the upper side of the sealing plate (34) is lower than the center of the piston tube (32); a pair of symmetrical vent holes (321) are opened on the inner wall of the piston tube (32) located inside the sealing plate (34).

5. A sampling device for detecting calf diarrhea pathogens according to claim 4, characterized in that: The rotating pipe fitting (31) includes a rotating pipe (311), a rotating plate (312), a cross rod (313), and a pair of sealing plates (314); the two ends of the rotating pipe (311) are rotatably connected to the adjusting pipe (13) and the connecting piston pipe (32), respectively; the rotating plate (312) is fixedly connected to the inner wall of the rotating pipe (311) near the end of the piston pipe (32), and its lower side extends beyond the center of the rotating pipe (311) and also beyond the upper side of the sealing plate (34); the cross rod (313) is fixedly connected to the rotating plate (312); a pair of sealing plates (314) are fixedly connected to the two ends of the cross rod (313) and the rotating pipe (311) in the vertical direction; the sealing plates (314) correspond to a pair of vent holes (321).

6. A sampling device for detecting pathogens causing calf diarrhea according to claim 5, characterized in that: The rotating pipe fitting (31) also includes a positioning block (35); the positioning block (35) is fixedly connected to the side of the closed plate (34) near the rotating plate (312); the positioning block (35) is lower than the upper side of the closed plate (34).

7. A sampling device for detecting pathogens causing calf diarrhea according to claim 1, characterized in that: Each sealing assembly (4) includes a telescopic rod (41), a heating tube (42), and a squeezing plate (43); a pair of squeezing plates (43) are located on the upper and lower sides of a rectangular through hole; both ends of each telescopic rod (41) are fixedly connected to the corresponding telescopic cavity (121) and the squeezing plate (43); a pair of heating tubes (42) are fixedly connected to the same side of the telescopic rod (41) for telescopic movement; both ends of the heating tubes (42) are fixedly connected to the connecting tube (5) and the output end of the infusion hole (11) respectively; the middle part of the connecting tube (5) is fixedly connected to the side of the rectangular through hole.

8. A sampling device for detecting pathogens causing calf diarrhea according to claim 7, characterized in that: The telescopic rod (41) is made of shape memory alloy; the deformation temperature of the shape memory alloy is set to 50-60°C.

9. A sampling device for detecting pathogens causing calf diarrhea according to claim 1, characterized in that: A cap (61) is inserted into the input end of one of the infusion ports (11); an injection tubing (62) is fixedly connected to the input end of the other infusion port (11); a plurality of vent holes (63) are also provided on the regulating tube (13), and the vent holes (63) are connected to the side wall of the input end of the other infusion port (11).

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