A type of antibacterial wipe and its manufacturing process

Abstract

This invention belongs to the field of wet wipe technology, specifically relating to a sterilizing wet wipe and its manufacturing process. The sterilizing wet wipe includes a wet wipe substrate and a wet wipe soaking solution; the wet wipe soaking solution comprises the following components by weight: 10-15 parts sterilizing ingredient; 0.5-1 part plant essential oil; 90-100 parts water; the sterilizing ingredient is composed of modified carboxymethyl chitosan and modified sodium hyaluronate in a mass ratio of 1-2:1; this sterilizing wet wipe achieves a sterilization rate of over 98% against Staphylococcus aureus, Escherichia coli, Candida albicans, and Bacillus subtilis within a short time, exhibiting excellent sterilization performance, while also providing good moisturizing effects, no skin irritation, and high safety.

Description

Technical Field

[0001] This invention belongs to the field of wet wipes technology, specifically relating to a bactericidal wet wipe and its manufacturing process. Background Technology

[0002] Wet wipes are a common personal hygiene product, usually made of non-woven fabric, paper towels, or cotton cloth, and contain a certain amount of moisture, moisturizers, and antibacterial, disinfectant, or skin-care ingredients. They are widely used in daily life. Common types of wet wipes are mainly divided into ordinary cleaning wipes, disinfecting wipes, baby wipes, and makeup remover wipes. Ordinary cleaning wipes are mainly used for daily cleaning of hands, face, or object surfaces, and usually contain mild surfactants that can effectively remove stains and grease. Disinfecting wipes, in addition to their cleaning function, also contain disinfectants such as alcohol and quaternary ammonium salts, which can kill or inhibit the growth of bacteria, viruses, and other microorganisms. Baby wipes are mainly used for daily cleaning of babies, such as wiping their buttocks and hands. They usually do not contain irritating ingredients such as alcohol and fragrances, and contain moisturizing ingredients such as glycerin and aloe vera. Makeup remover wipes contain makeup-removing ingredients such as surfactants and solvents, which can easily remove makeup and are suitable for quick makeup removal or travel use. As people's living standards continue to improve, the functional requirements for wet wipes are also increasing. Wet wipes with antibacterial, moisturizing, and low-irritation functions are gradually becoming a focus of attention.

[0003] Chinese patent application CN202410399749.1 discloses a disinfectant wipe and its preparation method. The disinfectant wipe contains a disinfectant agent comprising 0.4-2.4 wt% lactic acid and 0.02-0.1 wt% chlorhexidine compounds. The disinfectant agent is obtained by adding lactic acid and chlorhexidine compounds to water and stirring until homogeneous. Non-woven fabric is then soaked in the disinfectant agent to obtain the disinfectant wipe. While this patent uses lactic acid and chlorhexidine compounds as the disinfectant agent, and although the two components can synergistically improve the disinfection effect, the disinfectant wipe's disinfection effect is insufficient. Chinese patent application CN202410896513.9 discloses a long-lasting disinfectant wipe. This long-lasting disinfectant wipe uses functional small or large molecules and / or transition coupling agents or functional polymer materials as the functional main body and large or high molecular materials as the structural main body. It is made through processes such as spraying, dipping, soaking, bonding, and blending. However, the functional small or large molecules and / or transition coupling agents are organic chemical components, and some components are highly irritating to human skin, which limits their use. Therefore, it is necessary to develop a wipe with excellent sterilization performance and low irritation to meet market demand. Summary of the Invention

[0004] This invention addresses the shortcomings of existing technologies by providing a sterilizing wipe and its manufacturing process. The sterilizing wipe achieves a sterilization rate of over 98% against Staphylococcus aureus, Escherichia coli, Candida albicans, and Bacillus subtilis in a short time, demonstrating excellent sterilization performance. It also has good moisturizing effects, is non-irritating to the skin, and is highly safe.

[0005] The technical solution adopted in this invention is as follows:

[0006] A type of antibacterial wipe, comprising a wipe substrate and a wipe soaking solution; the wipe soaking solution comprises the following components by weight: 10-15 parts of antibacterial ingredient; 0.5-1 part of plant essential oil; and 90-100 parts of water; wherein the antibacterial ingredient is composed of modified carboxymethyl chitosan and modified sodium hyaluronate in a mass ratio of 1-2:1.

[0007] Furthermore, the plant essential oil is any one or a mixture of several of the following: rose essential oil, lavender essential oil, chamomile essential oil, and tea tree essential oil.

[0008] In the technical solution of this invention, the preparation method of the modified carboxymethyl chitosan is as follows: 50-60wt% ethanol solution and carboxymethyl chitosan are added to a reaction vessel and stirred evenly. The modified compound and triethylamine are added, and the mixture is stirred and reacted at a temperature of 50-60℃ for 5-6 hours to obtain modified carboxymethyl chitosan.

[0009] Furthermore, the mass ratio of the carboxymethyl chitosan, the modified compound, triethylamine, and 50-60 wt% ethanol solution is 1:0.4-0.5:0.08-0.1:25-30.

[0010] In the technical solution of the present invention, the preparation method of the modified sodium hyaluronate is as follows: water, sodium hyaluronate and sodium hydroxide are added to a reaction vessel, stirred evenly, a modified compound is added, and the mixture is stirred and reacted at a temperature of 60-70℃ for 8-10 hours to obtain modified sodium hyaluronate.

[0011] Furthermore, the mass ratio of sodium hyaluronate, modified compound, sodium hydroxide, and water is 1:0.5-0.6:0.1-0.15:15-20.

[0012] In the technical solution of this invention, the preparation methods of the modified compounds in the preparation methods of modified carboxymethyl chitosan and modified sodium hyaluronate are all as follows:

[0013] S1. Ethanol, 4-chloroaniline, and dodecyl dimethyl tertiary amine were added to a reaction vessel and refluxed at 80°C for 10-11 hours to obtain intermediate 1.

[0014] S2. Add chloroform and intermediate 1 to the reaction vessel, and slowly add epichlorohydrin dropwise. After the addition is complete, stir the reaction at a temperature of 30-40℃ for 3-5 hours to obtain intermediate 2.

[0015] S3. Toluene, intermediate 2, and guanidinoacetic acid are added to a reaction vessel, and concentrated sulfuric acid is added dropwise. After the addition is complete, the mixture is refluxed at 110°C for 6-8 hours to obtain the modified compound. The modified compound is:

[0016] .

[0017] Further, the molar ratio of 4-chloroaniline and dodecyl dimethyl tertiary amine in step S1 is 1:1.1-1.2.

[0018] Furthermore, in step S2, the molar ratio of intermediate 1 to epichlorohydrin is 1:1-1.1.

[0019] Further, in step S3, the molar ratio of intermediate 2, guanidinoacetic acid, and concentrated sulfuric acid is 1:1.1-1.2:0.05-0.1.

[0020] This invention also provides a manufacturing process for antibacterial wipes, comprising the following steps:

[0021] A1. Preparation of wet wipe soaking solution: According to the weight ratio, the bactericidal components of modified carboxymethyl chitosan and modified sodium hyaluronate, and plant essential oils are added to water and stirred evenly to obtain wet wipe soaking solution;

[0022] A2. Preparation of antibacterial wipes: Spray the wipe soaking solution onto the wipe substrate to obtain antibacterial wipes.

[0023] Furthermore, the stirring speed in step A1 is 500-600 r / min, and the stirring time is 20-30 min.

[0024] The present invention has the following beneficial effects:

[0025] To obtain antibacterial wipes with excellent sterilization properties, this invention modifies the antibacterial components in the wipe soaking solution. First, 4-chloroaniline is used as a raw material and reacted sequentially with dodecyl dimethyl tertiary amine, epichlorohydrin, and guanidinoacetic acid to obtain a modified compound. This modified compound contains a long-chain quaternary ammonium salt and guanidinyl and chloroalkyl groups. The long-chain quaternary ammonium salt and guanidinyl groups impart dual antibacterial effects to the modified compound. This invention also modifies carboxymethyl chitosan, which inherently possesses antibacterial properties. The amino groups in the carboxymethyl chitosan molecule react with the chloroalkyl groups in the modified compound to further enhance its antibacterial properties. Grafting the modified compound onto carboxymethyl chitosan molecules further enhances the bactericidal effect of carboxymethyl chitosan. On the other hand, sodium hyaluronate is modified by reacting the hydroxyl groups in the sodium hyaluronate molecule with the chloroalkyl groups in the modified compound, thereby grafting the modified compound onto the sodium hyaluronate molecule to obtain modified sodium hyaluronate with bactericidal properties. In this invention, modified carboxymethyl chitosan and modified sodium hyaluronate are combined as bactericidal components in bactericidal wipes. The synergistic effect of the two not only improves the moisturizing performance of the bactericidal wipes but also greatly enhances their bactericidal properties.

[0026] The antibacterial wipes provided by this invention can achieve a sterilization rate of over 98% against Staphylococcus aureus, Escherichia coli, Candida albicans, and Bacillus subtilis in a short time, demonstrating excellent sterilization performance. They also have good moisturizing effects, are non-irritating to the skin, and are highly safe, thus better meeting market needs. Detailed Implementation

[0027] The technical solutions in the embodiments of this application will be clearly and completely described below with reference to the embodiments of this application. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. Based on the embodiments of this application, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of this application.

[0028] In the technical solution of this invention, the raw materials used in the following embodiments and comparative examples are all commercially available products: sodium hyaluronate CAS number 9067-32-7 (purchased from Beijing Wokai Biotechnology Co., Ltd., product number A45224, molecular weight 3000), carboxymethyl chitosan CAS number 83512-85-0 (molecular weight 8.5kDa, degree of deacetylation 90%, degree of carboxymethylation 85%), sodium hydroxide CAS number 1310-73-2, and triethylamine CAS number 121-44-8. 4-Chloroaniline (CAS No. 106-47-8); dodecyl dimethyl tertiary amine (CAS No. 112-18-5); epichlorohydrin (CAS No. 106-89-8); guanidinoacetic acid (CAS No. 352-97-6); concentrated sulfuric acid (CAS No. 7664-93-9); ethanol (CAS No. 64-17-5); chloroform (CAS No. 67-66-3); toluene (CAS No. 108-88-3); diethyl ether (CAS No. 60-29-7); and acetone (CAS No. 67-64-1) are all analytical grade reagents.

[0029] Example 1

[0030] This embodiment provides a method for preparing a modified compound, and the synthetic route is as follows:

[0031] ;

[0032] S1. 500 mL of ethanol, 13.0 g of 4-chloroaniline, and 23.9 g of dodecyl dimethyl tertiary amine were added to a reaction vessel. The mixture was refluxed at 80 °C for 11 h. After the reaction was complete, the mixture was cooled, the ethanol was removed, diethyl ether was added, and the mixture was stirred, filtered, and the solid was dried to obtain 29.5 g of intermediate 1. The molar ratio of 4-chloroaniline to dodecyl dimethyl tertiary amine was 1:1.1. Intermediate 1: ESI (m / z): 341.3 [M+H] + , 1 H-NMR (600MHz, DMSO-d6, δppm): 6.85 (d, J=8.3Hz, 2H), 6.80 (d, J=8.3Hz, 2H), 5.50 (s, 2H), 3.74 (s, 6H), 3.23-3.27 (m, 2H), 1.72-1.78 (m, 2H), 1.24-1.31 (m, 18H), 0.88-0.93 (m, 3H);

[0033] S2. Add 400 mL of chloroform and 25.0 g of intermediate 1 to a reaction vessel, and slowly add 7.1 g of epichlorohydrin dropwise. After the addition is complete, stir the mixture at 35 °C for 5 h. After the reaction is complete, cool the mixture, remove the chloroform, add diethyl ether, stir, filter, and dry the solid to obtain 27.2 g of intermediate 2. The molar ratio of intermediate 1 to epichlorohydrin is 1:1.05. Intermediate 2: ESI (m / z): 433.3 [M+H] + , 1 H-NMR (600MHz, DMSO-d6, δppm): 9.53 (s, 1H), 6.90 (d, J=8.3Hz, 2H), 6.67 (d, J=8.3Hz, 2H), 5.37 (s, 1H), 3.64- 3.76 (m, 8H), 3.32-3.39 (m, 2H), 3.18-3.25 (m, 3H), 1.71-1.76 (m, 2H), 1.25-1.31 (m, 18H), 0.88-0.92 (m, 3H);

[0034] S3. Add 300 mL of toluene, 25.0 g of intermediate 2, and 7.4 g of guanidinoacetic acid to a reaction vessel. Add 0.3 g of concentrated sulfuric acid dropwise. After the addition is complete, reflux the reaction at 110 °C for 8 h. After the reaction is complete, cool, remove the toluene, add diethyl ether, stir, filter, and dry the solid to obtain 25.8 g of the modified compound. The molar ratio of intermediate 2, guanidinoacetic acid, and concentrated sulfuric acid is 1:1.1:0.05. Modified compound: ESI (m / z): 532.3 [M+H] + , 1 H-NMR (600MHz, DMSO-d6, δppm): 9.52 (s, 1H), 7.84 (s, 1H), 6.92 (d, J=8.3Hz, 2H), 6.68 (d, J=8.3Hz, 2H), 6.63 (s, 2H), 4.93-4.98 (m, 1H), 4. 32 (s, 2H), 3.70-3.76 (m, 7H), 3.40-3.48 (m, 2H), 3.15-3.23 (m, 3H), 2 .50 (s, 1H), 1.72-1.78 (m, 2H), 1.23-1.30 (m, 18H), 0.87-0.92 (m, 3H).

[0035] Example 2

[0036] This embodiment provides a sterilizing wipe and its manufacturing process.

[0037] A type of antibacterial wipe, comprising a wipe substrate and a wipe soaking solution; the wipe soaking solution comprises the following components by weight: 15 parts of antibacterial ingredient; 0.8 parts of plant essential oil; 100 parts of water; the antibacterial ingredient is composed of modified carboxymethyl chitosan and modified sodium hyaluronate in a mass ratio of 2:1; the plant essential oil is rose essential oil.

[0038] The modified carboxymethyl chitosan was prepared as follows: 60 wt% ethanol solution and carboxymethyl chitosan were added to a reaction vessel and stirred until homogeneous. The modified compound and triethylamine were then added, and the mixture was stirred at 55°C for 6 hours. After the reaction was completed, the mixture was cooled, and the pH of the solution was adjusted to neutral. The reaction solution was then added dropwise to acetone for precipitation. The mixture was filtered, and the solid was washed with acetone and dried to obtain the modified carboxymethyl chitosan. The degree of substitution of the modified compound was determined to be 12.6% by elemental analysis. The mass ratio of carboxymethyl chitosan, modified compound, triethylamine, and 60 wt% ethanol solution was 1:0.5:0.1:30.

[0039] The preparation method of modified sodium hyaluronate is as follows: water, sodium hyaluronate, and sodium hydroxide are added to a reaction vessel and stirred evenly. The modified compound is then added, and the mixture is stirred at 70℃ for 9 hours. After the reaction is completed, the mixture is cooled, and the reaction solution is added dropwise to anhydrous ethanol for precipitation. After filtration, the solid is washed with anhydrous ethanol and dried to obtain modified sodium hyaluronate. The degree of substitution of the modified compound is determined to be 15.4% by nuclear magnetic resonance hydrogen spectroscopy. The mass ratio of sodium hyaluronate, modified compound, sodium hydroxide, and water is 1:0.6:0.15:20.

[0040] A manufacturing process for antibacterial wipes includes the following steps:

[0041] A1. Preparation of wet wipe soaking solution: According to the weight ratio, the bactericidal components of modified carboxymethyl chitosan and modified sodium hyaluronate, and plant essential oils are added to water and stirred evenly to obtain wet wipe soaking solution; the stirring speed is 600 r / min and the stirring time is 20 min;

[0042] A2. Preparation of antibacterial wipes: Spray the wipe soaking solution onto the wipe substrate to obtain antibacterial wipes.

[0043] Example 3

[0044] This embodiment provides a sterilizing wipe and its manufacturing process.

[0045] A type of antibacterial wipe, comprising a wipe substrate and a wipe soaking liquid; the wipe soaking liquid comprises the following components by weight: 13 parts of antibacterial ingredient; 1 part of plant essential oil; 95 parts of water; the antibacterial ingredient is composed of modified carboxymethyl chitosan and modified sodium hyaluronate in a mass ratio of 1.5:1; the plant essential oil is tea tree oil.

[0046] The preparation method of modified carboxymethyl chitosan is as follows: 55wt% ethanol solution and carboxymethyl chitosan are added to a reaction vessel and stirred evenly. Modified compound and triethylamine are added, and the reaction is carried out at 60℃ for 5 hours. After the reaction is completed, the mixture is cooled, the pH of the solution is adjusted to neutral, and the reaction solution is added dropwise to acetone for precipitation. After filtration, the solid is washed with acetone and dried to obtain modified carboxymethyl chitosan. The degree of substitution of the modified compound is determined to be 12.0% by elemental analysis. The mass ratio of carboxymethyl chitosan, modified compound, triethylamine, and 55wt% ethanol solution is 1:0.45:0.09:25.

[0047] The preparation method of modified sodium hyaluronate is as follows: water, sodium hyaluronate, and sodium hydroxide are added to a reaction vessel and stirred evenly. The modified compound is then added, and the mixture is stirred and reacted at 60℃ for 10 hours. After the reaction is completed, the mixture is cooled, and the reaction solution is added dropwise to anhydrous ethanol for precipitation. After filtration, the solid is washed with anhydrous ethanol and dried to obtain modified sodium hyaluronate. The degree of substitution of the modified compound is determined to be 14.2% by nuclear magnetic resonance hydrogen spectroscopy. The mass ratio of sodium hyaluronate, modified compound, sodium hydroxide, and water is 1:0.5:0.1:15.

[0048] A manufacturing process for antibacterial wipes includes the following steps:

[0049] A1. Preparation of wet wipe soaking solution: According to the weight ratio, the bactericidal components of modified carboxymethyl chitosan and modified sodium hyaluronate, and plant essential oils are added to water and stirred evenly to obtain wet wipe soaking solution; the stirring speed is 500 r / min and the stirring time is 30 min;

[0050] A2. Preparation of antibacterial wipes: Spray the wipe soaking solution onto the wipe substrate to obtain antibacterial wipes.

[0051] Example 4

[0052] This embodiment provides a sterilizing wipe and its manufacturing process.

[0053] A type of antibacterial wipe, comprising a wipe substrate and a wipe soaking solution; the wipe soaking solution comprises the following components by weight: 10 parts of antibacterial ingredient; 0.5 parts of plant essential oil; 90 parts of water; the antibacterial ingredient is composed of modified carboxymethyl chitosan and modified sodium hyaluronate in a mass ratio of 1:1; the plant essential oil is chamomile essential oil.

[0054] The modified carboxymethyl chitosan was prepared as follows: 50 wt% ethanol solution and carboxymethyl chitosan were added to a reaction vessel and stirred until homogeneous. The modified compound and triethylamine were then added, and the mixture was stirred at 50 °C for 5.5 h. After the reaction was completed, the mixture was cooled, and the pH of the solution was adjusted to neutral. The reaction solution was then added dropwise to acetone for precipitation. After filtration, the solid was washed with acetone and dried to obtain the modified carboxymethyl chitosan. The degree of substitution of the modified compound was determined to be 11.3% by elemental analysis. The mass ratio of carboxymethyl chitosan, modified compound, triethylamine, and 50 wt% ethanol solution was 1:0.4:0.08:28.

[0055] The preparation method of modified sodium hyaluronate is as follows: water, sodium hyaluronate, and sodium hydroxide are added to a reaction vessel and stirred evenly. The modified compound is then added, and the mixture is stirred at 65℃ for 8 hours. After the reaction is completed, the mixture is cooled, and the reaction solution is added dropwise to anhydrous ethanol for precipitation. After filtration, the solid is washed with anhydrous ethanol and dried to obtain modified sodium hyaluronate. The degree of substitution of the modified compound is 14.8% as determined by 1H NMR spectroscopy. The mass ratio of sodium hyaluronate, modified compound, sodium hydroxide, and water is 1:0.55:0.13:18.

[0056] A manufacturing process for antibacterial wipes includes the following steps:

[0057] A1. Preparation of wet wipe soaking solution: According to the weight ratio, the bactericidal components of modified carboxymethyl chitosan and modified sodium hyaluronate, and plant essential oils are added to water and stirred evenly to obtain wet wipe soaking solution; the stirring speed is 550 r / min and the stirring time is 25 min.

[0058] A2. Preparation of antibacterial wipes: Spray the wipe soaking solution onto the wipe substrate to obtain antibacterial wipes.

[0059] Example 5

[0060] This embodiment provides a sterilizing wipe and its manufacturing process.

[0061] An antibacterial wipe, comprising a wipe substrate and a wipe soaking liquid; the wipe soaking liquid comprises the following components by weight: 14 parts of antibacterial ingredient; 0.7 parts of plant essential oil; 97 parts of water; the antibacterial ingredient is composed of modified carboxymethyl chitosan and modified sodium hyaluronate in a mass ratio of 1.8:1; the plant essential oil is lavender essential oil.

[0062] The modified carboxymethyl chitosan was prepared as follows: 60 wt% ethanol solution and carboxymethyl chitosan were added to a reaction vessel and stirred until homogeneous. The modified compound and triethylamine were then added, and the mixture was stirred at 60℃ for 6 hours. After the reaction was completed, the mixture was cooled, and the pH of the solution was adjusted to neutral. The reaction solution was then added dropwise to acetone for precipitation. The mixture was filtered, and the solid was washed with acetone and dried to obtain the modified carboxymethyl chitosan. The degree of substitution of the modified compound was determined to be 12.4% by elemental analysis. The mass ratio of carboxymethyl chitosan, modified compound, triethylamine, and 60 wt% ethanol solution was 1:0.5:0.08:30.

[0063] The preparation method of modified sodium hyaluronate is as follows: water, sodium hyaluronate, and sodium hydroxide are added to a reaction vessel and stirred evenly. The modified compound is then added, and the mixture is stirred at 70℃ for 8 hours. After the reaction is completed, the mixture is cooled, and the reaction solution is added dropwise to anhydrous ethanol for precipitation. After filtration, the solid is washed with anhydrous ethanol and dried to obtain modified sodium hyaluronate. The degree of substitution of the modified compound is 15.1% as determined by 1H NMR spectroscopy. The mass ratio of sodium hyaluronate, modified compound, sodium hydroxide, and water is 1:0.6:0.1:20.

[0064] A manufacturing process for antibacterial wipes includes the following steps:

[0065] A1. Preparation of wet wipe soaking solution: According to the weight ratio, the bactericidal components of modified carboxymethyl chitosan and modified sodium hyaluronate, and plant essential oils are added to water and stirred evenly to obtain wet wipe soaking solution; the stirring speed is 580 r / min and the stirring time is 24 min.

[0066] A2. Preparation of antibacterial wipes: Spray the wipe soaking solution onto the wipe substrate to obtain antibacterial wipes.

[0067] Comparative Example 1

[0068] This comparative example provides a sterilization wipe and its manufacturing process.

[0069] A type of antibacterial wipe, comprising a wipe substrate and a wipe soaking solution; the wipe soaking solution comprises the following components by weight: 15 parts of antibacterial ingredient; 0.8 parts of plant essential oil; 100 parts of water; the antibacterial ingredient is composed of carboxymethyl chitosan and modified sodium hyaluronate in a mass ratio of 2:1; the plant essential oil is rose essential oil.

[0070] The preparation method of modified sodium hyaluronate is the same as in Example 2.

[0071] The manufacturing process of one type of antibacterial wipe is the same as that in Example 2.

[0072] Comparative Example 2

[0073] This embodiment provides a sterilizing wipe and its manufacturing process.

[0074] A type of antibacterial wipe, comprising a wipe substrate and a wipe soaking solution; the wipe soaking solution comprises the following components by weight: 15 parts of antibacterial ingredient; 0.8 parts of plant essential oil; 100 parts of water; the antibacterial ingredient is composed of modified carboxymethyl chitosan and sodium hyaluronate in a mass ratio of 2:1; the plant essential oil is rose essential oil.

[0075] The preparation method of modified carboxymethyl chitosan is the same as in Example 2.

[0076] The manufacturing process of one type of antibacterial wipe is the same as that in Example 2.

[0077] Comparative Example 3

[0078] This embodiment provides a sterilizing wipe and its manufacturing process.

[0079] A type of antibacterial wipe, comprising a wipe substrate and a wipe soaking solution; the wipe soaking solution comprises the following components by weight: 15 parts of antibacterial ingredient; 0.8 parts of plant essential oil; 100 parts of water; the antibacterial ingredient is composed of carboxymethyl chitosan and sodium hyaluronate in a mass ratio of 2:1; the plant essential oil is rose essential oil.

[0080] The manufacturing process of one type of antibacterial wipe is the same as that in Example 2.

[0081] 1. Sterilization performance test of antibacterial wipes

[0082] The bactericidal performance of the antibacterial wipes prepared in Examples 2 to 5 and Comparative Examples 1 to 3 was tested according to Appendix C of GB / T15979-2002 Hygienic Standard for Disposable Sanitary Products. The test strains were Staphylococcus aureus (ATCC 6538), Escherichia coli (ATCC 25922), Candida albicans (ATCC 14053), and Bacillus subtilis (ATCC 6633), and the contact time was 2 min. The results are shown in Table 1 below.

[0083] Table 1. Results of bactericidal test

[0084]

[0085] As shown in Table 1, the antibacterial wipes prepared in Examples 2 to 4 of the present invention achieved a sterilization rate of over 98% against Staphylococcus aureus, Escherichia coli, Candida albicans, and Bacillus subtilis within a short period of time, indicating that the antibacterial wipes provided by the present invention have excellent sterilization performance. Compared with Comparative Examples 1 to 3, the antibacterial components in the antibacterial wipes of Example 2 of the present invention are modified carboxymethyl chitosan and modified sodium hyaluronate. Both are grafted with modified compound structures containing long-chain quaternary ammonium salts and guanidine groups with bactericidal effects. The two work synergistically to exert multiple bactericidal effects, thereby greatly enhancing the sterilization performance of the antibacterial wipes.

[0086] 2. Skin irritation and moisturizing properties test of antibacterial wipes

[0087] Thirty-five volunteers (aged 25-30, healthy, with no wounds on the back of their right hand) were randomly selected. They used the antibacterial wipes prepared in Examples 2-5 and Comparative Examples 1-3 twice daily, morning and evening, to wipe the back of their right hand for one week without using any moisturizing products. After use, the presence of allergic reactions such as erythema, papules, edema, itching, pain, or burning sensations on the back of the right hand was observed to assess the skin irritation of the antibacterial wipes. Additionally, the epidermal moisture content of the skin on the back of the right hand was measured using a skin moisture meter. Measurements were taken three times at the same location each time, and the average value was used. The moisturizing performance of the antibacterial wipes was evaluated by comparing the epidermal moisture content before and after use. The results are shown in Table 2 below.

[0088] Table 2 Results of skin irritation and moisturizing performance tests

[0089]

[0090] As shown in Table 2, the antibacterial wipes prepared in Examples 2 to 4 of the present invention are non-irritating to the skin and have good moisturizing effects. The antibacterial components in the antibacterial wipes prepared in the present invention are modified carboxymethyl chitosan and modified sodium hyaluronate, which can synergistically enhance the moisturizing performance of the antibacterial wipes and increase the moisture content of the skin epidermis.

[0091] It should be noted that, in this document, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Although embodiments of this application have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of this application, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A germicidal wet wipe characterized by, The bactericidal wet wipe comprises a wet wipe substrate and a wet wipe soaking solution; the wet wipe soaking solution comprises the following components in parts by weight: a bactericidal component 10-15 parts; a plant essential oil 0.5-1 part; water 90-100 parts; the bactericidal component is composed of modified carboxymethyl chitosan and modified sodium hyaluronate in a mass ratio of 1-2:1; The preparation method of the modified carboxymethyl chitosan is as follows: 50-60wt% ethanol solution, carboxymethyl chitosan are added into a reaction container, stirred uniformly, a modifying compound and triethylamine are added, stirring is carried out at a temperature of 50-60℃ for 5-6h, and the modified carboxymethyl chitosan is obtained; The preparation method of the modified sodium hyaluronate is as follows: water, sodium hyaluronate and sodium hydroxide are added into a reaction container, stirred uniformly, a modifying compound is added, stirring is carried out at a temperature of 60-70℃ for 8-10h, and the modified sodium hyaluronate is obtained; The preparation method of the modifying compound in the preparation methods of the modified carboxymethyl chitosan and the modified sodium hyaluronate is as follows: S1, ethanol, 4-chloroaniline and dodecyl dimethyl tertiary amine are added into a reaction container, refluxing reaction is carried out at a temperature of 80℃ for 10-11h, and the intermediate 1 is obtained; S2, chloroform, the intermediate 1 and slowly dropwise added epichlorohydrin are added into a reaction container, after dropwise addition, stirring reaction is carried out at a temperature of 30-40℃ for 3-5h, and the intermediate 2 is obtained; S3, toluene, the intermediate 2 and guanidino acetic acid are added into a reaction container, concentrated sulfuric acid is dropwise added, after dropwise addition, refluxing reaction is carried out at a temperature of 110℃ for 6-8h, and the modifying compound is obtained.

2. The germicidal wet wipe of claim 1 wherein, The molar ratio of the 4-chloroaniline and the dodecyl dimethyl tertiary amine in step S1 is 1:1.1-1.

2.

3. The germicidal wet wipe of claim 1 wherein, The molar ratio of the intermediate 1 and the epichlorohydrin in step S2 is 1:1-1.

1.

4. The germicidal wet wipe of claim 1 wherein, The molar ratio of the intermediate 2, the guanidino acetic acid and the concentrated sulfuric acid in step S3 is 1:1.1-1.2:0.05-0.

1.

5. The germicidal wet wipe of claim 1 wherein, The mass ratio of the carboxymethyl chitosan, the modifying compound, triethylamine and 50-60wt% ethanol solution in the preparation method of the modified carboxymethyl chitosan is 1:0.4-0.5:0.08-0.1:25-30.

6. The germicidal wet wipe of claim 1 wherein, The mass ratio of the sodium hyaluronate, the modifying compound, sodium hydroxide and water in the preparation method of the modified sodium hyaluronate is 1:0.5-0.6:0.1-0.15:15-20.

7. The germicidal wet wipe of claim 1 wherein, The plant essential oil is any one or a mixture of several of rose essential oil, lavender essential oil, chamomile essential oil and tea tree essential oil.

8. The process of claim 1-7, wherein the process further comprises, The method comprises the following steps: A1, preparation of the wet wipe soaking solution: according to the weight ratio, the bactericidal component of the modified carboxymethyl chitosan and the modified sodium hyaluronate and the plant essential oil are added into water, stirred uniformly, and the wet wipe soaking solution is obtained; A2, preparation of the bactericidal wet wipe: the wet wipe soaking solution is sprayed on the wet wipe substrate, and the bactericidal wet wipe is obtained.

9. The process of claim 8 wherein the process further comprises the step of: The stirring speed in step A1 is 500-600r / min, and the stirring time is 20-30min.

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