Sunshool microcapsule as well as preparation method and application thereof
By coating with macromolecular materials and preparing marsaicin microcapsules by coagulation method, the staining and stability of marsaicin is solved, and its efficient application and safe storage in skin preparations are achieved.
Patent Information
- Application Number
- CN202510182542.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-19
- Publication Date
- 2025-05-30
AI Technical Summary
Yasaicin is prone to dyeing and unstable, limiting its application in skin preparations and affecting its storage and transportation conditions.
The marsaicin microcapsules were prepared by coagulation using macromolecular materials such as gelatin or hyaluronic acid, which solved the staining and stability of marsaicin.
Through microencapsulation, the fluidity, dispersion and biocompatibility of yamsaicin are improved, the activity cycle is extended, the generation of photofree radicals is reduced, the safety is improved, and the scope of application is broadened.
Smart Images
Figure CN120053306A_ABST
Abstract
Description
Technical Field
[0001] This application relates to the technical field of natural small molecule modification, and particularly relates to a sanshool microcapsule, a preparation method thereof, and an application thereof. Background Art
[0002] In recent years, the treatment of photoaging mediated by inhibiting inflammatory stress response through natural products and their active ingredients has attracted extensive attention. Chinese prickly ash is the fruit of shrubs or arbors of the genus Zanthoxylum in the Rutaceae family, also known as Qinjiao, Chuanjiao, or sanshool; in addition to being used as a seasoning, Chinese prickly ash can also extract a variety of amide compounds, alkaloids, lignans, flavonoids, etc., and these substances have a wide range of medicinal values, including functions such as anthelmintic, antibacterial, inhibiting platelet aggregation, and cytotoxicity.
[0003] Sanshool is a kind of amide substance extracted from Chinese prickly ash and has a characteristic long conjugated structure. Sanshool has a wide range of anti-inflammatory, anti-microbial, antioxidant, and sensory neuron activation effects. In recent years, there have also been research reports on the application of sanshool in skin beauty. For example, topical creams containing sanshool can effectively improve facial wrinkles. In addition, studies have shown that sanshool can reduce the expression of ROS in fibroblasts, thereby protecting cells from UVB-induced photo-damage.
[0004] However, sanshool itself has a bright yellow color, which will stain fabrics and skin, and its application in skin preparations is restricted by its strong color characteristics; in addition, due to the instability of sanshool, its storage conditions are extremely harsh, and there are certain obstacles in the clinical application prospects.
[0005] Based on this, systematic research on improving the staining problem of sanshool and enhancing its stability is of great significance for broadening the application scenarios of sanshool and increasing its added value. Summary of the Invention
[0006] This application provides a sanshool microcapsule, a preparation method thereof, and an application thereof, aiming to solve the problems of easy staining and low stability existing in existing sanshool.
[0007] To achieve the above object, this application adopts the following technical solutions to be realized.
[0008] In the first aspect of this application, a preparation method of a sanshool microcapsule is provided, which uses a macromolecular material as a coating and adopts a coacervation method to microencapsulate sanshool to obtain a sanshool microcapsule.
[0009] In some embodiments, the preparation method includes:
[0010] S1, dissolving the macromolecular material in hot water, adding a flocculant and stirring, cooling to room temperature to obtain an upper clear liquid and a lower coacervate layer, and collecting the coacervate layer;
[0011] S2. Dissolve the coacervate layer in water to obtain a shell solution, disperse capsicum in the shell solution, stir and mix; then add a formaldehyde solution with the same volume as the coacervate layer and stir. After the reaction, a crude microcapsule product is obtained.
[0012] S3. Wash the crude microcapsule product, disperse it in water, and perform freeze-drying after freezing to obtain capsicum microcapsules.
[0013] In some embodiments, the macromolecular material is gelatin or hyaluronic acid.
[0014] In some embodiments, the flocculant is a mixture of ethanol and acetone.
[0015] In some embodiments, the mass ratio of the macromolecular material, capsicum, ethanol, and acetone is 1:(0.5 - 1.5):(5 - 6):(3 - 4).
[0016] In some embodiments, the water temperature of the hot water in S1 is 45 - 55°C.
[0017] In some embodiments, the washing in S3 is specifically: washing the crude microcapsules with ethanol at room temperature and then washing with water at 0 - 5°C.
[0018] In some embodiments, the temperature of the freezing is -70°C and the freezing time is 24 h.
[0019] In the second aspect of the present application, capsicum microcapsules prepared by the above preparation method are provided.
[0020] In the third aspect of the present application, the application of capsicum microcapsules prepared by the above preparation method in skin care products is provided.
[0021] Compared with the prior art, the beneficial effects of the present application are as follows:
[0022] By designing the material structure and using the coacervation method to prepare capsicum microcapsules, the present application solves the problems of easy staining and instability of capsicum. The process is simple and suitable for large-scale production. Through microencapsulation, the present application improves the fluidity, dispersibility, and biocompatibility of capsicum, making it have high chemical activity, effectively protecting the skin from severe photo-damage, and improving the effect of skin care products. In addition, through microencapsulation, the generation of photo-free radicals can be effectively reduced, the potential risk of raw materials is reduced, and the safety is improved.
[0023] The capsicum microcapsules prepared by the present application effectively alleviate the staining problem of capsicum, broaden the application range of capsicum; and have good photo-stability, increase the active period of capsicum, and are convenient for storage and transportation. Description of the Drawings
[0024] To more clearly illustrate the technical solutions of the embodiments of the present application, the accompanying drawings required for the description of the embodiments will be briefly introduced below. Obviously, the accompanying drawings in the following description are only some embodiments recorded in the present application. For those of ordinary skill in the art, without creative efforts, other accompanying drawings can also be obtained based on these drawings.
[0025] Figure 1 SEM image of the capsicum oleoresin microcapsules prepared in the present application;
[0026] Figure 2 Graph of the test results of the light absorption retention rate of capsicum oleoresin and capsicum oleoresin microcapsules;
[0027] Figure 3 Graph of the test results of the free radical scavenging rate of capsicum oleoresin and capsicum oleoresin microcapsules under dark conditions;
[0028] Figure 4 Graph of the test results of the free radical scavenging rate of capsicum oleoresin and capsicum oleoresin microcapsules under light conditions;
[0029] Figure 5 Graph of the test results of the antioxidant stability of capsicum oleoresin and capsicum oleoresin microcapsules;
[0030] Figure 6 Graph of the test results of the cell viability of capsicum oleoresin and capsicum oleoresin microcapsules;
[0031] Figure 7 Graph of the relative fluorescence intensity of ROS of capsicum oleoresin and capsicum oleoresin microcapsules;
[0032] Figure 8 Graph of the average skin thickness results of mice after light exposure;
[0033] Figure 9 Graph of the skin injury score results of mice. Specific Embodiments
[0034] Next, the technical solutions in the embodiments of the present application will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, rather than all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present application.
[0035] In the following description of this embodiment, the terms "include", "comprise", "have", "contain", etc. are all open-ended terms, that is, they are intended to include but not limited to.
[0036] In the following description of this embodiment, the term "and / or" is used to describe the association relationship of associated objects, indicating that there can be three relationships. For example, A and / or B can represent: A exists alone, B exists alone, and the situation where A and B exist simultaneously. Where A and B can be singular or plural. The character " / " generally indicates that the associated objects before and after are in an "or" relationship.
[0037] In the following description of this embodiment, the term "at least one" means one or more, and "a plurality" means two or more. "At least one of the following" or its similar expressions refer to any combination of these items, including any combination of single item(s) or plural item(s). For example, "at least one of a, b or c", or, "at least one of a, b and c" can both represent: a, b, c, a - b (i.e., a and b), a - c, b - c, or a - b - c, where a, b, c can be single or multiple respectively.
[0038] The terms used in the embodiments of this application are only for the purpose of describing specific embodiments and are not intended to limit this application. The singular forms "a" and "the" used in the embodiments of this application and the appended claims are also intended to include the plural forms unless the context clearly indicates otherwise.
[0039] Those skilled in the art should understand that in the following description of the embodiments of this application, the sequence numbers do not imply the order of execution. Some or all of the steps can be executed in parallel or sequentially, and the execution order of each process should be determined by its function and internal logic, and should not constitute any limitation to the implementation process of the embodiments of this application.
[0040] Those skilled in the art should understand that the numerical ranges in the embodiments of this application should be understood as specifically disclosing each intermediate value between the upper and lower limits of the range. Each intermediate value within any stated value or stated range, as well as each smaller range between any other stated value or intermediate value within the stated range, is also included in this application. The upper and lower limits of these smaller ranges can be independently included or excluded from the range.
[0041] Unless otherwise specified, the technical / scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the art to which this application belongs. Although this application only describes preferred methods and materials, any methods and materials similar or equivalent to those described herein can also be used in the implementation or testing of this application. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials related to the documents. In case of conflict with any incorporated document, the content of this specification shall prevail.
[0042] In a first aspect, the present application provides a method for preparing capsicin microcapsules, which uses a macromolecular material as a coating and encapsulates capsicin by a coacervation method to obtain capsicin microcapsules.
[0043] The capsicin microcapsules prepared in the present application have a core-shell structure, with its outer shell being a macromolecular material and the core being capsicin, thus solving the staining problem caused by direct contact of capsicin, and improving the light stability of capsicin based on the protection of the outer shell of the macromolecular material. Among them, the macromolecular material has high biocompatibility and high safety. In the present application, the macromolecular material is selected from gelatin or hyaluronic acid, preferably gelatin.
[0044] By designing the material structure and using the coacervation method to prepare capsicin microcapsules, the present application solves the problems of easy staining and instability of capsicin, and the process is simple and suitable for large-scale production. Specifically, the method for preparing capsicin microcapsules includes:
[0045] S1, Dissolve the macromolecular material in hot water, add a flocculant and stir, and cool to room temperature to obtain a supernatant and a lower coacervate layer, and collect the coacervate layer;
[0046] In the present application, first dissolve the macromolecular material, such as gelatin, in water at a temperature of 45-55 °C, preferably the temperature of water is 50 °C, to obtain a gelatin solution. Add a mixture of ethanol and acetone as a flocculant to the gelatin solution, and continuously stir to promote the coacervation of the gelatin solution. After the solution is cooled to room temperature, the solution is layered. The upper layer is the supernatant, mainly composed of water, and part of ethanol and acetone; the lower layer is the coacervate layer, mainly composed of gelatin, and part of ethanol and acetone.
[0047] S2, Dissolve the coacervate layer in water to obtain a shell solution, disperse capsicin in the shell solution, and stir and mix; then add a formaldehyde solution with the same volume as the coacervate layer and stir, and obtain a crude microcapsule product after the reaction;
[0048] Among them, the amount of water used in the shell solution is 4-6 times the amount of gelatin used, preferably 5 times. Disperse capsicin in the shell solution, stir and mix at a temperature of 1-10 °C for 1-2 h to ensure that capsicin is evenly dispersed in the shell solution and capsicin is coated by the shell solution.
[0049] After that, add a formaldehyde solution with the same volume as the coacervate layer to the shell solution, stir at a speed of 500-2000 r / min for 1-3 h to make the formaldehyde react with the shell solution and promote the hardening of the shell solution to form a shell. After the shell solution on the surface of capsicin hardens, capsicin is wrapped in it to form a crude microcapsule product. Among them, the volume concentration of the formaldehyde solution is 37%.
[0050] S3. After washing the crude microcapsule product, disperse it in water, and obtain the sanshool microcapsule through freezing and lyophilization drying.
[0051] Wash the crude microcapsule product with ethanol at room temperature 3 - 5 times first, and then wash it with water at 0 - 5°C. Disperse the washed crude microcapsule product in water, freeze it at -70°C for 12 - 24 h, and then remove the water through lyophilization treatment to obtain the sanshool microcapsule.
[0052] In this application, the dosages of the macromolecular material, sanshool, ethanol, and acetone have an impact on the final formation of the sanshool microcapsule. Preferably, the mass ratio of the macromolecular material, sanshool, ethanol, and acetone is 1:(0.5 - 1.5):(5 - 6):(3 - 4).
[0053] The sanshool microcapsule prepared in this application effectively alleviates the staining problem of sanshool, broadens the application range of sanshool; and has good light stability, increases the active period of sanshool, and is convenient for storage and transportation. Through microencapsulation, the fluidity, dispersibility, and biocompatibility of sanshool are improved, and it has a certain slow-release effect, making it have high chemical activity, which can effectively protect the skin from severe light damage and improve the effect of skin care products. In addition, through microencapsulation, the generation of light free radicals can be effectively reduced, the potential risk of raw materials is reduced, and the safety is improved.
[0054] The sanshool microcapsule prepared in this application does not cause staining problems, has good light stability, is convenient for storage and transportation, and has high safety, making it particularly suitable for skin care products, can be used to prepare cosmetics with whitening effects, and can effectively improve facial wrinkles.
[0055] The following further illustrates this application through examples.
[0056] In the examples of this application, the materials or substances used can be obtained by purchasing commodities. Among them, the gelatin is of the Adamas brand, with the specification of type B, bloom 225, and is from Shanghai Titan Scientific Co., Ltd.
[0057] Example 1
[0058] Dissolve 1 part of gelatin in 10 parts of distilled water at a temperature of 50°C, add 5 parts of ethanol and 4 parts of acetone, and continuously stir for 5 min. After cooling to room temperature, the solution is divided into an upper clear liquid and a lower coagulation layer, and collect the coagulation layer;
[0059] Dissolve the coacervate layer in 5 parts of distilled water to obtain the shell solution; disperse 0.5 part of capsicin into the shell solution, and use a magnetic hotplate stirrer to stir for 1 h at 10 °C and 500 r / min. Add a formaldehyde solution with a concentration of 37% (V / V) having the same volume as the coacervate layer to the shell solution, and stir to react to harden the gelatin coating, obtaining a crude microcapsule product. Collect the crude microcapsule product, wash it 3 times with ethanol, then wash it with water at 5 °C, and then disperse the washed crude microcapsule product in water, freeze it at -70 °C for 24 h, and then remove the moisture by freeze-drying to obtain capsicin microcapsules, denoted as S1.
[0060] Example 2
[0061] Dissolve 1 part of gelatin in 10 parts of distilled water at 50 °C, add 5 parts of ethanol and 4 parts of acetone, and continuously stir for 5 min. After cooling to room temperature, the solution is divided into an upper clear liquid and a lower coacervate layer, and collect the coacervate layer;
[0062] Dissolve the coacervate layer in 5 parts of distilled water to obtain the shell solution; disperse 1 part of capsicin into the shell solution, and use a magnetic hotplate stirrer to stir for 1 h at 10 °C and 500 r / min. Add a formaldehyde solution with a concentration of 37% (V / V) having the same volume as the coacervate layer to the shell solution, and stir to react to harden the gelatin coating, obtaining a crude microcapsule product. Collect the crude microcapsule product, wash it 3 times with ethanol, then wash it with water at 5 °C, and then disperse the washed crude microcapsule product in water, freeze it at -70 °C for 24 h, and then remove the moisture by freeze-drying to obtain capsicin microcapsules, denoted as S2.
[0063] Example 3
[0064] Dissolve 1 part of gelatin in 10 parts of distilled water at 50 °C, add 5 parts of ethanol and 4 parts of acetone, and continuously stir for 5 min. After cooling to room temperature, the solution is divided into an upper clear liquid and a lower coacervate layer, and collect the coacervate layer;
[0065] Dissolve the coacervate layer in 5 parts of distilled water to obtain the shell solution; disperse 2 parts of capsicin into the shell solution, and use a magnetic hotplate stirrer to stir for 1 h at 10 °C and 500 r / min. Add a formaldehyde solution with a concentration of 37% (V / V) having the same volume as the coacervate layer to the shell solution, and stir to react to harden the gelatin coating, obtaining a crude microcapsule product. Collect the crude microcapsule product, wash it 3 times with ethanol, then wash it with water at 5 °C, and then disperse the washed crude microcapsule product in water, freeze it at -70 °C for 24 h, and then remove the moisture by freeze-drying to obtain capsicin microcapsules, denoted as S3.
[0066] Example 4
[0067] Dissolve 1 part of gelatin in 10 parts of distilled water at 50 °C, add 6 parts of ethanol and 3 parts of acetone, and continuously stir for 5 min. After cooling to room temperature, the solution is divided into an upper clear liquid and a lower coagulation layer, and the coagulation layer is collected;
[0068] Dissolve the coagulation layer in 5 parts of distilled water to obtain a shell solution; disperse 0.5 part of sanshool into the shell solution, and use a magnetic hot plate stirrer to stir at 10 °C and 500 r / min for 1 h. Add a formaldehyde solution with a concentration of 37% (V / V) equal to the volume of the coagulation layer to the shell solution, and stir to react to harden the gelatin coating to obtain a crude microcapsule product. Collect the crude microcapsule product, wash it 3 times with ethanol, then wash it with water at 5 °C, and then disperse the washed crude microcapsule product in water, freeze it at -70 °C for 24 h, and then remove the moisture by freeze-drying to obtain sanshool microcapsules, denoted as S4.
[0069] Perform performance evaluations on the sanshool microcapsules S1 - S3 prepared in Examples 1 - 3, specifically including:
[0070] 1. Particle size test
[0071] Dissolve the sanshool microcapsules S1, S2, and S3 in water respectively to prepare sample solutions with a concentration of 200 μg / ml, spin-coat them on the surface of a smooth silicon wafer, and successively perform drying and gold spraying treatments, and observe them using a bench-top scanning electron microscope. The morphological results are as Figure 1 shown. Among them, Figure 1 The left side is the morphological diagram of S1, Figure 1 The middle is the morphological diagram of S2, Figure 1 The right side is the morphological diagram of S3.
[0072] From Figure 1 it can be seen that the three kinds of sanshool microcapsules are ellipsoidal, and the particle size of the material is between 500 nm and 2 μm, with good dispersibility.
[0073] 2. Dyeing property test
[0074] Paste white adhesive paper labels (4×4 cm) on an aluminum tray, evenly apply the sanshool microcapsules S1, S2, and S3 on the paper labels respectively, and evenly apply sanshool powder with the same amount of sanshool as that in S1, S2, and S3 on the paper labels as a comparison, place them in a sealed container and store for 12 hours, and then invert the tray and gently tap to take out the powder or microcapsule samples.
[0075] The sanshool microcapsules are easy to remove and leave a light stain on the paper label. On the contrary, the sanshool powder tends to aggregate and adhere to the paper label, and obvious dark yellow stains will be left even when gently tapping the sample tray. It shows that the sanshool microencapsulation strategy can effectively alleviate the dyeing problem of the material and broaden its application range.
[0076] 3. Light stability test
[0077] Dissolve capsaisin microcapsules S1, S2, and S3 in water respectively to prepare a solution with a concentration of 50 μg / mL; dissolve capsaisin powder in ethanol to prepare a solution with a concentration of 50 μg / mL. The above are respectively denoted as the capsaisin group, S1, S2, and S3 groups.
[0078] Use a PerkinElmer Lambda 650 ultraviolet / visible spectrophotometer to record the ultraviolet-visible spectra of the above four groups of solutions, and set the slit to 2 nm. At 271 nm, the original absorbance of each group is denoted as A0; irradiate each group with simulated sunlight (2 W) for 120 min, and then measure the absorbance of each group, denoted as At.
[0079] Evaluate the light stability by calculating the light absorption retention rate based on the light absorption ability of each group before and after simulated sunlight irradiation. The formula for calculating the light absorption retention rate is At / A0 * 100%, and the test results are as Figure 2 shown.
[0080] From Figure 2 it can be seen that the capsaisin group has obvious light instability. As the irradiation time prolongs, the absorption ability of capsaisin gradually decreases. After 120 min of irradiation, the light absorption ability retention rate is only about 50%, indicating that the light stability of capsaisin is poor. However, for the capsaisin microcapsules S1, S2, and S3 prepared with gelatin as the coating and ethanol and acetone as the coagulants, the change in light absorption ability before and after irradiation is less than that of the capsaisin group, proving that their light stability is better than that of capsaisin.
[0081] 4. Antioxidant test
[0082] Prepare a DPPH solution with a concentration of 1 mg / mL using absolute ethanol and 1,1-diphenyl-2-picrylhydrazyl (DPPH); then take samples of capsaisin microcapsules S1, S2, S3, and capsaisin powder respectively, and dissolve them in ethanol to prepare samples with a concentration of 0.6 mg / L.
[0083] Add 300 μL of DPPH solution and 100 μL of sample solution to 2600 μL of ethanol. Measure the absorbance at 517 nm under the conditions of no light and simulated sunlight (2 W) irradiation, and calculate the free radical scavenging rate of each group of samples after 30 min. The calculation formula is:
[0084] Free radical scavenging rate = (A0 - A) / A0 * 100%
[0085] where A0 is the absorbance at the 0th min, and A is the absorbance at the 30th min.
[0086] Under the conditions of no light and light, the free radical scavenging rate is asFigure 3 and Figure 4 as shown. From Figure 3 and Figure 4 it can be seen that the sanshools microcapsules S1, S2, and S3 all have good free radical scavenging ability, and their free radical scavenging rates are all higher than that of sanshools, showing good antioxidant effects.
[0087] The antioxidant stability of the above-mentioned groups of samples was tested as follows:
[0088] Record the free radical scavenging rate of each group of samples at 30 min in the absence of light as W 0 , and record the free radical scavenging rate of each group of samples at 30 min under light as W t . Calculate its antioxidant stability through the formula W t / W 0 *100%, and the results are as Figure 5 shown. Due to its inherent molecular properties, sanshools has certain antioxidant ability, but its stability is poor. The prepared sanshools microcapsules S1, S2, and S3 have stronger free radical scavenging ability and antioxidant stability than sanshools.
[0089] 5. Biocompatibility test
[0090] Using HaCaT cells as the cell line, the CCK-8 method was used to detect the effects of sanshools microcapsules S1, S2, S3 and sanshools on the proliferation activity of HaCaT cells before and after light exposure.
[0091] First, prepare a sanshools solution diluted with medium at a concentration of 50 μg / mL and sample solutions of sanshools microcapsules S1, S2, and S3 diluted with medium at a concentration of 50 μg / mL.
[0092] The cells were cultured by adding 10% fetal bovine serum (FBS) to MEM medium and incubating together. The culture atmosphere was a humid atmosphere containing 5% CO 2 . The temperature was maintained at 37°C. The cultured HaCaT cells were incubated in a 96-well plate at a density of 2000 cells per well for 24 h, and then the medium, the above-mentioned sanshools solution, and the above-mentioned sample solutions of sanshools microcapsules S1, S2, and S3 were added respectively. In the absence of light and under simulated sunlight (2 W) irradiation for 2 min, incubation was continued for 24 h, and the corresponding cell viability was detected according to the CCK-8 method test instructions. The detection results are shown in Figure 6 . Among them, the group added with medium was the control group.
[0093] From Figure 6 it can be seen that the sanshools microcapsules S1, S2, and S3 all have good biocompatibility and good biological application prospects.
[0094] 6. Measuring the ROS level of HaCaT cells
[0095] First, prepare capsaicin solution diluted with culture medium at a concentration of 100 μg / mL, and sample solutions of capsaicin microcapsules S1, S2, and S3 diluted with culture medium at a concentration of 100 μg / mL.
[0096] In a confocal dish, inoculate HaCaT cells evenly at a concentration of 1.5×10 5 cells / mL. After incubation for 24 h, add culture medium, culture medium, the above capsaicin solution, and the above sample solutions of capsaicin microcapsules S1, S2, and S3 respectively, and then process for another 24 h. Among them, one group added with culture medium is used as the control group without light irradiation; the rest are irradiated with 2W ultraviolet light for 2 min and then continue to incubate for 24 h, and then stained with Hoechst33258 and DCFH-DA. Observe the expression of ROS in cells of each group through a Leica Stellaris laser confocal microscope, and the fluorescence statistical results are as Figure 7 shown. Among them, the group added with culture medium and irradiated with light is defined as the UVB group.
[0097] From Figure 7 , it can be seen that compared with the positive control, the fluorescence intensities of the groups treated with capsaicin and materials S1, S2, and S3 all decreased, indicating that they all have a certain light protection ability. However, capsaicin itself will also cause some stimulation under light irradiation, resulting in a still relatively high fluorescence intensity of the cells, while the fluorescence intensities of the cells treated with materials S1, S2, and S3 are relatively low, indicating their good cell protection effect.
[0098] 7. Photo-damage test
[0099] The experimental mice used in this experiment: female, with a body weight of about 25 g, purchased from Chengdu Shuoda Animal Company.
[0100] Dissolve capsaicin in ethanol to prepare a capsaicin solution with a concentration of 2 mg / ml; dissolve capsaicin microcapsules S1, S2, and S3 in water respectively to prepare sample solutions with a concentration of 2 mg / ml.
[0101] Select 24 experimental mice and divide them into 6 groups, namely the untreated control group (C), the light irradiation group (UVB), the capsaicin group (S), and the groups S1, S2, and S3 treated with capsaicin microcapsules. Before daily light irradiation, in the capsaicin group (S) and groups S1, S2, and S3, apply 2 mg / ml capsaicin and sample solutions of S1, S2, and S3 on the back skin of the mice respectively, 0.3 ml each time; the light irradiation group (UVB) only applies an equal amount of clear water. Irradiate the back skin of the mice in the remaining groups except the control group with ultraviolet light (2W) for 5 min once a day for 5 days. Record the back skin thickness and damage score of the six groups of mice every day, as shown in Figure 8 andFigure 9 as shown
[0102] From Figure 8 and Figure 9 it can be seen that the skin thickness of the mice applied with sanshool and sanshool microcapsules S1, S2, and S3 is significantly greater than that of the control group, indicating that sanshool, S1, S2, and S3 all have a protective effect on the mice. Among them, the mice applied with S2 have the lowest degree of damage and have a good light protection effect.
[0103] Although the present application has been described in detail with general descriptions and specific embodiments in this specification, on the basis of the present application, some modifications or improvements can be made, which are obvious to those skilled in the art. Therefore, these modifications or improvements made without departing from the spirit of the present application all fall within the scope claimed by the present application.
Claims
1. A method for preparing sanshool microcapsules, characterized in that: The method uses macromolecular materials as coating and adopts a coagulation method to microencapsulate sanshool to obtain sanshool microcapsules.
2. The preparation method according to claim 1, characterized in that: include: S1, dissolving the macromolecular material in hot water, adding a flocculant and stirring, cooling to room temperature to obtain an upper clear liquid and a lower coacervate, and collecting the coacervate; S2, dissolving the coacervate in water to obtain a shell solution, dispersing sanshool in the shell solution, stirring and mixing; then adding a formaldehyde solution of the same volume as the coacervate, stirring, and obtaining a crude microcapsule product after reaction; S3, washing the crude microcapsule product, dispersing it in water, freezing it, and freeze-drying it to obtain sanshool microcapsules.
3. The preparation method according to claim 2, characterized in that: The macromolecular material is gelatin or hyaluronic acid.
4. The preparation method according to claim 2, characterized in that: The flocculant is a mixture of ethanol and acetone.
5. The preparation method according to claim 4, characterized in that: The mass ratio of the macromolecular material, sanshool, ethanol and acetone is 1:(0.5-1.5):(5-6):(3-4).
6. The preparation method according to claim 2, characterized in that: In S1, the water temperature of the hot water is 45-55°C.
7. The preparation method according to claim 2, characterized in that: The washing in S3 specifically includes: washing the crude product with ethanol at room temperature, and then washing with water at 0-5°C.
8. The preparation method according to claim 2, characterized in that: The freezing temperature is -70°C and the freezing time is 24h.
9. Sanshool microcapsules prepared by the preparation method described in any one of claims 1 to 8.
10. Use of the sanshool microcapsules prepared by the preparation method according to any one of claims 1 to 8 in skin care products.