Liposome coated sanshool nano material as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of natural molecular modification, and discloses a liposome-coated sanshool nano material as well as a preparation method and application thereof. The preparation method comprises the following steps: dissolving soybean lecithin, cholesterol, Tween 80 and sanshool in an organic solvent to obtain a solution A; dissolving polyphenol in a PBS (Phosphate Buffer Solution) to obtain a solution B; dropwise adding the solution A into the solution B, and stirring at 30-40 DEG C; then heating to 45 DEG C, and evaporating to remove the solvent to obtain a precursor solution; adding a PBS buffer solution into the precursor solution, and adjusting the volume of the PBS buffer solution to be the same as that of the solution B; and carrying out ultrasonic treatment to obtain the lipidosome coated sanshool nano material. The lipidosome-coated sanshool nano-material prepared by the invention is nanoscale, has good light stability, is not easy to inactivate, and can be stored for a long time; the ultraviolet absorption capability is good, and the invasion of ultraviolet rays can be effectively resisted; good biocompatibility and water solubility are achieved, and activity and safety are high; and the product has good free radical scavenging ability and good oxidation resistance.

Description

Technical Field

[0001] This application belongs to the technical field of natural molecule modification, and specifically relates to a liposome-encapsulated sanshool nanomaterial, its preparation method and application. Background Art

[0002] Sanshool is a type of amide substance extracted from Chinese prickly ash. Its characteristic long conjugated carbon chain structure endows it with excellent physical and chemical properties, especially in its ultraviolet absorption ability and free radical scavenging ability. Sanshool has functions such as antioxidant, anti-inflammatory, anti-microbial, and activating sensory neurons. At the same time, it also has good biocompatibility. In addition to providing a moderately spicy taste for Chinese prickly ash, it can also effectively improve facial wrinkles. Currently, it has been widely used in fields such as food, health products, biomedicine, and cosmetics.

[0003] Research shows that sanshool can show photoprotective effects on UVB-induced photo-damaged skin by reducing the level of reactive oxygen species (ROS) in fibroblasts. It can increase the cell viability of cells exposed to UVB, reduce UVB-induced fibroblast photo-damage by inhibiting the production of MMP, induce autophagy, and inhibit the JAK2-STAT3 pathway. The increase in autophagy fails to promote apoptosis, indicating that sanshool can balance autophagy and apoptosis and can effectively prevent human skin damage caused by UVB. Currently, sanshool has been applied to the preparation of skin care products for repairing skin photo-damage and resisting ultraviolet invasion, and has achieved relatively good results, showing good development and application prospects.

[0004] However, the presence of intramolecular double bonds in the structure of sanshool leads to its instability. It is extremely easy to isomerize or even break down under ultraviolet light irradiation, resulting in inactivation and performance degradation. In addition, sanshool also has skin penetration problems, which hinder its application in the prevention and treatment of skin photo-damage. It is necessary to seek appropriate methods to enhance the photoprotective ability of sanshool and improve the stability of sanshool, which is beneficial to its application in fields such as skin photo-damage treatment and oxidative stress diseases.

[0005] Liposome is a nano-spherical vesicle, which is composed of an inner aqueous phase and one or more layers of phospholipid bilayers that wrap the inner aqueous phase. It is formed by the self-aggregation of concentric phospholipid bilayers dispersed in the aqueous phase, and its diameter distribution is between dozens of nanometers and several micrometers. Liposome is an ultrafine spherical porous particle with a vesicle structure similar to the cell membrane and high biocompatibility. Its amphiphilic structure can encapsulate hydrophobic active ingredients in the hydrophobic cavity, or hydrophilic active ingredients in the hydrophilic cavity, or simultaneously encapsulate hydrophobic and hydrophilic active ingredients in the hydrophobic and hydrophilic cavities respectively. The membrane material of liposome is a natural raw material, with the advantages of high biocompatibility and safety.

[0006] Natural polyphenols are common secondary metabolites in plants and have functions such as antioxidant, antibacterial, anti-tumor, neuroprotective, regulating blood lipid, and promoting gastrointestinal health. Natural polyphenols can enhance the ultraviolet protection stability of capsaicin itself, improve the light stability of capsaicin, and achieve antioxidant synergistic effects, thus broadening the application potential and application scenarios of capsaicin materials.

[0007] Based on this, it is of great significance to construct an assembled material by combining capsaicin, liposome and natural polyphenols to improve the stability and solubility of capsaicin. Summary of the Invention

[0008] This application provides a liposome-encapsulated capsaicin nanomaterial, its preparation method and application, aiming to solve the problems of low stability and poor water solubility of capsaicin in the prior art.

[0009] To achieve the above object, the following technical solutions are adopted in this application.

[0010] In the first aspect of this application, a preparation method of a liposome-encapsulated capsaicin nanomaterial is provided, including:

[0011] S1, dissolving soybean lecithin, cholesterol, Tween 80 and capsaicin in an organic solvent to obtain solution A; dissolving polyphenols in PBS buffer to obtain solution B;

[0012] S2, dropwise adding solution A into solution B and stirring at 30-40 °C; then raising the temperature to 45 °C to evaporate the solvent to obtain a precursor solution;

[0013] S3, adding PBS buffer to the precursor solution to adjust its volume to be the same as the volume of solution B;

[0014] S4, ultrasonically treating the liquid prepared in S3 to obtain a liposome-encapsulated capsaicin nanomaterial.

[0015] In some embodiments, the polyphenol is any one or a combination of epigallocatechin gallate, procyanidin or epicatechin.

[0016] In some embodiments, the mass ratio of soybean lecithin, cholesterol, Tween 80, capsaicin and polyphenol is 15:3-5:3-5:1-3:0-3.

[0017] In some embodiments, in solution A, the dosage of soybean lecithin corresponds to the volume of the organic solvent at 4 mg / mL; in solution B, the volume of the PBS buffer is the same as the volume of the organic solvent.

[0018] In some embodiments, the organic solvent is at least one of methanol, ethanol or chloroform.

[0019] In some embodiments, the concentration of the PBS buffer is 0.01 M and the pH is 6.8.

[0020] In some embodiments, the sonication is performed using a sonicator cell disruptor with a power of 100 W and a temperature of 0 - 5 °C.

[0021] In a second aspect of the present application, there is provided a nanomaterial of capsicin encapsulated in liposomes prepared by the above preparation method.

[0022] In a third aspect of the present application, there is provided the use of a nanomaterial of capsicin encapsulated in liposomes as a free radical scavenger and antioxidant.

[0023] In a fourth aspect of the present application, there is provided the use of a nanomaterial of capsicin encapsulated in liposomes in skin care products.

[0024] Compared with the prior art, the beneficial effects of the present application are as follows:

[0025] The preparation method of the present application can quickly and effectively assemble capsicin, polyphenols and liposomes to form a nanomaterial. Its process is simple and has good controllability, making it suitable for large-scale production.

[0026] The nanomaterial of capsicin encapsulated in liposomes prepared in the present application is nanoscale, with a uniform and regular morphology, good light stability, not easily inactivated, and can be stored for a long time; it has good ultraviolet absorption ability and can effectively resist ultraviolet radiation; it has good biocompatibility and water solubility, high activity and safety; it has good free radical scavenging ability and good antioxidant property.

[0027] The nanomaterial of capsicin encapsulated in liposomes of the present application can be used for the treatment of skin photo-damage and oxidative stress diseases, with high activity and good safety. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required for the description of the embodiments will be briefly introduced below. Obviously, the drawings in the following description are only some embodiments described in the present application. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.

[0029] Figure 1 is the SEM image of the nanomaterial of capsicin encapsulated in liposomes;

[0030] Figure 2 is the test result graph of the Zeta potential of the nanomaterial of capsicin encapsulated in liposomes;

[0031] Figure 3 is the ultraviolet absorption spectrum of the nanomaterial of capsicin encapsulated in liposomes;

[0032] Figure 4 It is a graph showing the results of the photostability test of the liposome-encapsulated sanshool nanomaterial L-S;

[0033] Figure 5 It is a graph showing the results of the photostability test of sanshool;

[0034] Figure 6 It is a graph showing the results of the free radical scavenging performance (antioxidant performance) test of the liposome-encapsulated sanshool nanomaterial;

[0035] Figure 7 It is a graph showing the results of the biocompatibility test of the liposome-encapsulated sanshool nanomaterial; Detailed implementation manners

[0036] Next, the technical solutions in the embodiments of the present application will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present application.

[0037] In the following description of this embodiment, the terms "include", "comprise", "have" and "contain" are all open-ended terms, that is, they are intended to include but not limited to.

[0038] In the following description of this embodiment, the term "and / or" is used to describe the association relationship of associated objects, indicating that there can be three relationships. For example, A and / or B can represent: A exists alone, B exists alone, and both A and B exist at the same time. Where A and B can be singular or plural. The character " / " generally represents an "or" relationship between the associated objects before and after.

[0039] In the following description of this embodiment, the term "at least one" means one or more, and "a plurality" means two or more. "At least one of the following items" or its similar expressions refer to any combination of these items, including any combination of single item or plural items. For example, "at least one of a, b or c", or, "at least one of a, b and c" can all represent: a, b, c, a-b (that is, a and b), a-c, b-c, or a-b-c, where a, b, c can be single or multiple respectively.

[0040] The terms used in the embodiments of the present application are only for the purpose of describing specific embodiments, and are not intended to limit the present application. The singular forms of "a" and "the" used in the embodiments of the present application and the appended claims are also intended to include the plural forms, unless the context clearly indicates otherwise.

[0041] Those skilled in the art should understand that in the following description of the embodiments of the present application, the sequence numbers do not imply the order of execution. Some or all of the steps can be executed in parallel or sequentially. The execution order of each process should be determined by its function and internal logic, and should not constitute any limitation to the implementation process of the embodiments of the present application.

[0042] Those skilled in the art should understand that the numerical range in the embodiments of the present application should be understood as specifically disclosing each intermediate value between the upper and lower limits of the range. The intermediate value within any stated value or stated range, as well as each smaller range between any other stated value or intermediate value within the stated range, is also included in the present application. The upper and lower limits of these smaller ranges can be independently included or excluded from the range.

[0043] Unless otherwise specified, the technical / scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the art to which the present application belongs. Although the present application only describes preferred methods and materials, any methods and materials similar or equivalent to those described herein can also be used in the implementation or testing of the present application. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials related to the documents. In case of conflict with any incorporated document, the content of this specification shall prevail.

[0044] In a first aspect, the present application provides a method for preparing a liposome-encapsulated sanshool nanomaterial, comprising:

[0045] S1, dissolving soybean lecithin, cholesterol, Tween 80 and sanshool in an organic solvent to obtain solution A; dissolving polyphenol in PBS buffer to obtain solution B;

[0046] In the present application, the organic solvent is at least one of methanol, ethanol or chloroform, such as methanol, ethanol, chloroform and a mixture of ethanol, and the preferred organic solvent is ethanol; the polyphenol can be any one or a combination of epigallocatechin gallate, procyanidin or epicatechin; the concentration of the PBS buffer is 0.01M and the pH is 6.8.

[0047] In the present application, the mass ratio of soybean lecithin, cholesterol, emulsifier, sanshool and polyphenol is preferably 15:3-5:3-5:1-3:0-3, and the mass ratio of soybean lecithin, cholesterol, emulsifier, sanshool and polyphenol is particularly preferably 15:3:3:1:1. Among them, the dosage of the organic solvent is in a proportional relationship with the dosage of soybean lecithin. Specifically, the corresponding relationship between the dosage of soybean lecithin and the volume of the organic solvent is 4mg / mL, that is, for every 4mg of soybean lecithin, the volume of the organic solvent required is 1mL; among them, the volume of the PBS buffer is the same as the volume of the organic solvent.

[0048] S2, slowly add solution A drop by drop into solution B, and stir at 30 - 40 °C; then raise the temperature to 45 °C to evaporate the solvent to obtain a precursor solution.

[0049] Specifically, at 30 - 40 °C, slowly add solution A drop by drop into solution B while stirring; after the addition, continue stirring for 10 - 30 min to ensure thorough mixing. Heat the mixture to 45 °C to evaporate the organic solvent. In this application, a rotary evaporator is preferably used to evaporate the organic solvent.

[0050] S3, add PBS buffer to the precursor solution to adjust its volume to be the same as that of solution B.

[0051] S4, ultrasonically treat the liquid prepared in S3 to obtain the nanomaterial of capsaisin encapsulated by liposomes.

[0052] Specifically, use an ultrasonic cell disruptor to ultrasonically treat the solution obtained in S3 at a power of 100 W and a temperature of 0 - 5 °C for 30 - 60 min to obtain a homogeneous colloid, which is the nanomaterial of capsaisin encapsulated by liposomes.

[0053] The nanomaterial of capsaisin encapsulated by liposomes prepared in this application is a transparent colloid. Its dispersed particle size is at the nanoscale, its morphology is uniform and regular, it has good light stability, high activity and can be stored for a long time; it has good ultraviolet absorption ability and can effectively resist ultraviolet radiation; it has good water solubility and biocompatibility, high activity and safety; and it has good free radical scavenging ability and good antioxidant property, and it can be used as a free radical scavenger and antioxidant.

[0054] The nanomaterial of capsaisin encapsulated by liposomes of this application can be used for the treatment of skin photo - damage and oxidative stress diseases. It has high activity and good safety and can be used in the preparation of skin care products.

[0055] The following further illustrates this application through examples.

[0056] Example 1

[0057] This application provides a preparation method of the nanomaterial of capsaisin encapsulated by liposomes, including:

[0058] S1, dissolve 60 mg of soy lecithin, 12 mg of cholesterol, 12 mg of Tween 80, and 4 mg of capsaisin in 15 mL of absolute ethanol to obtain solution A.

[0059] S2, slowly add solution A drop by drop into a PBS buffer with a concentration of 0.01 M and a pH of 6.8 at 35 °C, and continuously stir at 35 °C for 20 minutes; then raise the temperature to 45 °C and rotary evaporate to remove ethanol to obtain a precursor solution.

[0060] S3. Add PBS buffer with a concentration of 0.01 M and a pH of 6.8 to the precursor solution until its volume reaches 15 mL, and stir evenly.

[0061] S4. Ultrasonically treat the solution prepared in S3 with an ultrasonic cell disruptor at a temperature of 5 °C and a power of 100 W for 30 min to obtain the capsicum extract nano-material encapsulated by liposomes, denoted as L-S.

[0062] Example 2

[0063] This application provides a method for preparing a capsicum extract nano-material encapsulated by liposomes, including:

[0064] S1. Dissolve 60 mg of soybean lecithin, 12 mg of cholesterol, 12 mg of Tween 80, and 4 mg of capsicum extract in 15 mL of ethanol to obtain solution A; dissolve 4 mg of epigallocatechin gallate in 15 mL of PBS buffer with a concentration of 0.01 M and a pH of 6.8 to obtain solution B.

[0065] S2. Slowly add solution A dropwise to solution B at 35 °C, and then continuously stir the mixture at 35 °C for 20 minutes; then raise the temperature to 45 °C and rotary evaporate to remove ethanol to obtain the precursor solution.

[0066] S3. Add PBS buffer with a concentration of 0.01 M and a pH of 6.8 to the precursor solution until its volume reaches 15 mL, and stir evenly.

[0067] S4. Ultrasonically treat the solution prepared in S3 with an ultrasonic cell disruptor at a temperature of 5 °C and a power of 100 W for 30 min to obtain the capsicum extract nano-material encapsulated by liposomes, denoted as L-S-EGCG.

[0068] Example 3

[0069] This application provides a method for preparing a capsicum extract nano-material encapsulated by liposomes, including:

[0070] S1. Dissolve 60 mg of soybean lecithin, 12 mg of cholesterol, 12 mg of Tween 80, and 4 mg of capsicum extract in 15 mL of ethanol to obtain solution A; dissolve 4 mg of procyanidins in 15 mL of PBS buffer with a concentration of 0.01 M and a pH of 6.8 to obtain solution B.

[0071] S2. Slowly add solution A dropwise to solution B at 35 °C, and then continuously stir the mixture at 35 °C for 20 minutes; then raise the temperature to 45 °C and rotary evaporate to remove ethanol to obtain the precursor solution.

[0072] S3. Add PBS buffer with a concentration of 0.01 M and a pH of 6.8 to the precursor solution until its volume reaches 15 mL, and stir evenly.

[0073] S4. Ultrasonically treat the solution prepared in S3 for 30 min with an ultrasonic cell disruptor under the conditions of a temperature of 5 °C and a power of 100 W to obtain a nanomaterial of capsicin encapsulated in liposomes, denoted as L-S-OPC.

[0074] Example 4

[0075] This application provides a method for preparing a nanomaterial of capsicin encapsulated in liposomes, including:

[0076] S1. Dissolve 60 mg of soybean lecithin, 12 mg of cholesterol, 12 mg of Tween 80, and 4 mg of capsicin in 15 mL of ethanol to obtain Solution A; dissolve 4 mg of epicatechin in 15 mL of PBS buffer solution with a concentration of 0.01 M and a pH of 6.8 to obtain Solution B.

[0077] S2. Dropwise add Solution A to Solution B at 35 °C, and then continuously stir the mixture at 35 °C for 20 minutes; then raise the temperature to 45 °C and rotary evaporate to remove ethanol to obtain a precursor solution.

[0078] S3. Add PBS buffer solution with a concentration of 0.01 M and a pH of 6.8 to the precursor solution until its volume reaches 15 mL, and stir evenly.

[0079] S4. Ultrasonically treat the solution prepared in S3 for 30 min with an ultrasonic cell disruptor under the conditions of a temperature of 5 °C and a power of 100 W to obtain a nanomaterial of capsicin encapsulated in liposomes, denoted as L-S-EC.

[0080] Perform performance evaluations on the nanomaterials of capsicin encapsulated in liposomes prepared in Examples 1-4 as follows:

[0081] 1. Particle size test

[0082] Prepare aqueous solutions with a concentration of 1 mg / mL of the nanomaterials of capsicin encapsulated in liposomes prepared in Examples 1-4 respectively, spin-coat them on the surface of a smooth mica sheet, and observe with a scanning electron microscope after drying and gold spraying in sequence. Their SEM images are as Figure 1 shown.

[0083] From Figure 1It can be seen that both capsicin and natural polyphenols form good nano-assemblies after co-encapsulation, and the assembled morphology is spherical. Further, by statistically analyzing their particle sizes, it can be obtained that the particle size of the liposome-encapsulated capsicin nanomaterial (L-S) obtained by assembling pure capsicin with liposomes is 118 nm ± 9 nm, the particle size of the liposome-encapsulated capsicin nanomaterial (L-S-EGCG) obtained by co-encapsulating capsicin with EGCG is 102 nm ± 6 nm, the particle size of the liposome-encapsulated capsicin nanomaterial (L-S-OPC) obtained by co-encapsulating capsicin with OPC is 135 nm ± 5 nm, and the particle size of the liposome-encapsulated capsicin nanomaterial obtained by co-encapsulating capsicin with EC is 140 nm ± 9 nm. It can be seen that the liposome-encapsulated capsicin nanomaterials have a narrow particle size distribution and relatively similar particle sizes.

[0084] 2. Zeta potential value

[0085] The liposome-encapsulated capsicin nanomaterials prepared in Examples 1-4 were respectively formulated into aqueous solutions with a concentration of 20 μg / ml and tested using a nanoparticle size and Zeta potential analyzer. The obtained Zeta potential test results are as Figure 2 shown.

[0086] Zeta potential test is to test and statistically analyze the shear plane potential of the particle solution. When the absolute value of the potential obtained by Zeta test is larger, the corresponding solution has stronger stability. It can be seen from Figure 2 that the solutions of the liposome-encapsulated capsicin nanomaterials prepared in this application all have a high negative charge, indicating that the materials all have high stability.

[0087] 3. Ultraviolet light absorption ability

[0088] The liposome-encapsulated capsicin nanomaterials prepared in Examples 1-4 were formulated into aqueous solutions with a concentration of 1 mg / mL, and the ultraviolet absorption of the samples in the wavelength range of 200-400 nm was measured using an ultraviolet-visible spectrophotometer, with a slit width of 2 nm. The test results are as Figure 3 shown. Characteristic absorption peaks of capsicin can be observed in the wavelength range of 250-300 nm. The above liposome-encapsulated capsicin nanomaterials all have good absorption in the ultraviolet band, especially better absorption in the UVB band, and all have good ultraviolet absorption ability.

[0089] 4. Stability test of spectral absorption in the ultraviolet region

[0090] The liposome-encapsulated sanshool nanomaterial L-S prepared in Example 1 was formulated into an aqueous solution with a concentration of 2 mg / mL, and its ultraviolet absorption in the wavelength range of 200 - 400 nm was measured using an ultraviolet-visible spectrophotometer, with a slit width of 2 nm. Then, L-S was irradiated under simulated sunlight (2 W) for 30 min, 1 h, and 2 h, and its ultraviolet absorbance values in the wavelength range of 200 - 400 nm were measured again. The photostability of L-S after sunlight irradiation was observed and compared. The results are as follows Figure 4 shown

[0091] For comparison, another pure sanshool was prepared into an ethanol solution with a concentration of 30 μg / mL, and its ultraviolet absorption in the wavelength range of 200 - 400 nm was measured using an ultraviolet-visible spectrophotometer, with a slit width of 2 nm. Then, the sanshool was irradiated under simulated sunlight (2 W) for 30 min, 1 h, and 2 h, and its ultraviolet absorbance values in the wavelength range of 200 - 400 nm were measured again. The photostability of sanshool after sunlight irradiation was observed and compared. The results are as follows Figure 5 shown

[0092] From Figure 4 and Figure 5 it can be seen that pure sanshool has low photostability, while the photostability of the liposome-encapsulated sanshool nanomaterial L-S is significantly improved

[0093] 6. Free radical scavenging performance test

[0094] The 2,2-diphenyl-1-picrylhydrazyl (DPPH) method was used to evaluate the in vitro DPPH free radical scavenging ability of the liposome-encapsulated sanshool nanomaterials prepared in Examples 1 - 4 and pure sanshool. The specific method is as follows

[0095] Sample solutions with a concentration of 5 mg / mL were prepared using the liposome-encapsulated sanshool nanomaterials prepared in Examples 1 - 4 and pure sanshool respectively, and a DPPH ethanol solution with a concentration of 0.1 mmol / L was also prepared

[0096] Take 2300 μL of ethanol, add 200 μL of the DPPH ethanol solution, and then add 500 μL of the sample solution. After mixing for 2 h, the absorbance of the mixed solution at 517 nm was measured using an ultraviolet-visible spectrophotometer; take 2800 μL of ethanol, add 200 μL of the DPPH ethanol solution, and after mixing for 2 h, the absorbance of the mixed solution at 517 nm was measured using an ultraviolet-visible spectrophotometer; the free radical scavenging rate of the material was obtained from the two absorbances to evaluate the antioxidant ability of the ethanol phase of each group of samples

[0097] The test results are as Figure 6 shown. From Figure 6It can be seen that both sanshool and the sanshool-loaded liposome nanomaterials have certain antioxidant properties. Among them, the liposome-encapsulated sanshool nanomaterials obtained by co-encapsulating polyphenols and sanshool in liposomes, such as L-S-EGCG, L-S-OPC, and L-S-EC prepared in Examples 2-4, have more excellent antioxidant properties.

[0098] 7. Biocompatibility Test

[0099] Samples with concentrations of 50 μg / mL and 100 μg / mL were prepared from sanshool and the liposome-encapsulated sanshool nanomaterials prepared in Examples 1-4. Using NIH mouse embryo fibroblast 3T3 cells as the cell line, the cytotoxicity of the above samples was tested by the Alamar Blue test method.

[0100] The cells were cultured by adding 10% fetal bovine serum (FBS) to DMEM medium and incubating together. The culture atmosphere was a humid atmosphere containing 5% CO 2 , and the temperature was maintained at 37 °C. The cultured NIH 3T3 cells were incubated in a 96-well plate at a density of 2000 cells per well for 24 h, and then treated with the above samples at concentrations of 50 μg / mL and 100 μg / mL for another 24 h. Then, the corresponding cell survival rate was detected according to the Alamar Blue test instructions. The detection results are shown in Figure 7 .

[0101] From Figure 7 it can be seen that the cell survival rate is relatively low after treatment with sanshool, and even lower at high concentrations. The cell compatibility has been significantly improved after treatment with the liposome-encapsulated sanshool nanomaterials, demonstrating its good biosafety.

[0102] Although this application has been described in detail with general descriptions and specific embodiments in this specification, based on this application, some modifications or improvements can be made, which are obvious to those skilled in the art. Therefore, these modifications or improvements made without departing from the spirit of this application all fall within the scope claimed by this application.

Claims

1. A method for preparing a liposome-encapsulated sanshool nanomaterial, characterized in that: include: S1, dissolving soybean lecithin, cholesterol, Tween 80 and sanshool in an organic solvent to obtain solution A; Dissolve polyphenols in PBS buffer to obtain solution B; S2, adding solution A dropwise into solution B, stirring at 30-40°C; then heating to 45°C to evaporate and remove the solvent, to obtain a precursor solution; S3, add PBS buffer to the precursor solution and adjust its volume to the same volume as solution B; S4, subjecting the liquid prepared in S3 to ultrasonic treatment to obtain liposome-encapsulated sanshool nanomaterials.

2. The preparation method according to claim 1, characterized in that: The polyphenol is any one or more combinations of epigallocatechin gallate, proanthocyanidins or epicatechin.

3. The preparation method according to claim 1, characterized in that: The mass ratio of soybean lecithin, cholesterol, Tween 80, sanshool and polyphenol is 15:3~5:3~5:1~3:0~3.

4. The preparation method according to claim 1, characterized in that: In solution A, the corresponding relationship between the amount of soybean lecithin and the volume of the organic solvent is 4 mg / mL; in solution B, the volume of the PBS buffer is the same as the volume of the organic solvent.

5. The preparation method according to claim 1, characterized in that: The organic solvent is at least one of methanol, ethanol or chloroform.

6. The preparation method according to claim 1, characterized in that: The concentration of the PBS buffer is 0.01 M and the pH is 6.

8.

7. The preparation method according to claim 1, characterized in that: The ultrasonic treatment uses an ultrasonic cell crusher with a power of 100W and a temperature of 0-5°C.

8. The liposome-encapsulated sanshool nanomaterial prepared by the preparation method described in any one of claims 1 to 7.

9. Use of the liposome-encapsulated sanshool nanomaterial according to claim 8 as a free radical scavenger and antioxidant.

10. Use of the liposome-encapsulated sanshool nanomaterial according to claim 8 in skin care products.

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