Hair-fixing and hair-loss-preventing composition, application thereof and hair-fixing and hair-loss-preventing product

Abstract

The invention discloses a hair-fixing and hair-loss-preventing composition, application thereof and a hair-fixing and hair-loss-preventing product. The hair-fixing and hair-loss-preventing composition comprises 17-type collagen, a plant extract and carnitine substances, wherein the plant extract comprises a cacumen biotae extract. On the basis of inhibiting 5alpha-reductase and resisting oxidation, the composition disclosed by the invention also has the effects of fixing hair and preventing hair loss by virtue of ways of relieving inflammatory response, activating hair papilla cells, maintaining health of the hair papilla cells, reducing hair follicle senescence cells and the like.

Description

Technical Field

[0001] The present invention relates to the field of cosmetics, and particularly to a hair-fixing and anti-hair loss composition, its application, and a hair-fixing and anti-hair loss product. Background Art

[0002] The problem of hair loss is multi-dimensional, and its impact is not limited to the appearance level, but more widely involves multiple aspects such as mental health, social activities, economic status, and medical health. From a psychological perspective, hair loss can significantly reduce an individual's self-esteem and self-image perception, thereby inducing negative psychological states such as anxiety and depression, having an adverse impact on social activities, and in severe cases, may even lead to social isolation. At the same time, gender identity and personal attractiveness may also be impacted by hair loss. In the field of sociology, the negative impact of hair loss on career development cannot be ignored, especially in those professional fields with strict appearance requirements, where the impact is more significant. In addition, hair loss may also interfere with the evaluation of self-confidence and attractiveness in interpersonal relationships, reducing the overall quality of life. Economically, the financial burden brought by hair loss treatment cannot be ignored, including the high costs of hair transplantation and special hair care products.

[0003] Hair loss is a complex physiological phenomenon affected by multiple factors. There are many reasons for hair loss. External environments such as ultraviolet rays, chemical substances, and heat treatment can all damage the hair and exacerbate hair loss. Research shows that nutritional status is also crucial for hair health. Lack of nutrients such as protein, iron, zinc, and vitamins will directly affect hair growth and quality. At the same time, certain systemic diseases, such as thyroid diseases and autoimmune diseases, are also closely related to hair loss. Drug use is also a major inducement of hair loss. Many drugs such as chemotherapy drugs and antidepressants may cause hair loss. Scalp health problems cannot be underestimated either. Microbiological imbalance, fungal infection, and inflammatory diseases can all lead to hair loss. Psychological factors such as stress and anxiety indirectly affect hair growth by influencing hormone levels and the immune system. Physical damage, poor hair care habits, and physiological processes such as pregnancy and childbirth may also cause hair loss. Finally, as people age, the hair growth cycle changes, which is also an important reason for hair loss.

[0004] Genetic factors are also key factors affecting hair loss. Especially in androgenetic alopecia, genetic susceptibility plays a decisive role. Changes in hormone levels cannot be ignored either. For example, an increase in androgens can trigger follicular atrophy, thereby leading to hair loss. Hair follicle stem cells play a key role in the hair growth cycle. The hair growth cycle includes the anagen, catagen, and telogen phases. Among them, hair follicle stem cells are activated during the anagen phase to promote the growth of new hair. The decline or disorder of the function of hair follicle stem cells can cause hair loss because they cannot maintain the anagen phase of the hair follicle, resulting in sparse and falling hair. Especially in androgenetic alopecia, androgens may interfere with hair follicle stem cells, triggering follicular atrophy and hair thinning.

[0005] At present, there are also a large number of anti-hair loss compositions and formulations on the market. Chinese Patent Application CN118845516A discloses an anti-hair loss composition, which is composed of adenosine, coffee extract, L-carnitine tartrate, tripeptide-1 copper, Platycladus orientalis extract and niacinamide. This composition has a significant synergistic effect in improving cell antioxidant capacity and inhibiting 5α-reductase. However, this composition has no obvious effect on inhibiting the senescence or disorder of hair follicle stem cells and maintaining the growth phase of hair follicles. Summary of the Invention

[0006] In order to solve the above problems, the present invention provides an anti-hair loss and hair-strengthening composition and its application, as well as an anti-hair loss and hair-strengthening product. On the basis of inhibiting 5α-reductase and antioxidant, the composition of the present invention also exerts the effects of hair strengthening and anti-hair loss through reducing inflammatory response, activating dermal papilla cells, maintaining the health of dermal papilla cells and reducing senescent cells in hair follicles.

[0007] To achieve the above technical objectives, the present invention provides the following technical solutions.

[0008] In a first aspect, the present invention provides an anti-hair loss and hair-strengthening composition, which comprises type XVII collagen, plant extract and carnitine substances, and the plant extract includes Platycladus orientalis extract.

[0009] In the present invention, the type XVII collagen can be obtained by conventional chemical synthesis methods or genetic engineering methods in the art.

[0010] In some embodiments, the molecular weight of the type XVII collagen is 20-200 kDa, such as 23.8 kDa, 97 kDa, 120 kDa or 180 kDa.

[0011] In the present invention, the Platycladus orientalis extract can be obtained by extracting with the branches or branches and leaves of the plant Platycladus orientalis (L.) Franco of the family Cupressaceae. The extraction method can be conventional in the art.

[0012] In the present invention, the carnitine substances can be selected from one or more of carnitine, carnitine hydrochloride, acetyl carnitine hydrochloride, carnitine tartrate and palmitoyl carnitine.

[0013] In some embodiments, the carnitine substance is carnitine tartrate.

[0014] In the present invention, the carnitine tartrate is also known as L-carnitine tartrate, and can be obtained by reacting L-carnitine with tartaric acid, and its chemical formula is C 18 H 36 N 2 O12 , with a molecular weight of 472.48 and a CAS No. of 36687 - 82 - 8.

[0015] In some embodiments, the mass ratio of type XVII collagen, the extract of Platycladus orientalis leaves, and the carnitine substances is (0.0001 - 0.1) : (0.1 - 5) : (0.003 - 0.5), for example, 1:100:3.

[0016] In some embodiments, the hair - strengthening and anti - hair - loss composition comprises type XVII collagen, the extract of Platycladus orientalis leaves, and L - carnitine tartrate.

[0017] In some embodiments, the hair - strengthening and anti - hair - loss composition consists of type XVII collagen, the extract of Platycladus orientalis leaves, and L - carnitine tartrate.

[0018] In the above - mentioned embodiments, preferably, the mass ratio of type XVII collagen, the extract of Platycladus orientalis leaves, and L - carnitine tartrate is (0.0001 - 0.1) : (0.1 - 5) : (0.003 - 0.5), for example, 1:100:3.

[0019] In some embodiments, the hair - strengthening and anti - hair - loss composition further comprises adenosine substances, and the adenosine substances are preferably selected from one or more of disodium adenosine triphosphate, cyclic adenosine monophosphate, adenosine phosphate, adenosine triphosphate, and adenosine, for example, adenosine.

[0020] In the present invention, the adenosine can be formed by connecting N - 9 of adenine and C - 1 of D - ribose through a β - glycosidic bond, with the chemical formula C 10 H 13 N 5 O 4 , with a molecular weight of 267.24 and a CAS No. of 58 - 61 - 7.

[0021] In some embodiments, the hair - strengthening and anti - hair - loss composition comprises type XVII collagen, the extract of Platycladus orientalis leaves, adenosine, and L - carnitine tartrate.

[0022] In some embodiments, the hair - strengthening and anti - hair - loss composition consists of type XVII collagen, the extract of Platycladus orientalis leaves, adenosine, and L - carnitine tartrate.

[0023] In the above - mentioned embodiments, preferably, the mass ratio of type XVII collagen, the extract of Platycladus orientalis leaves, adenosine, and L - carnitine tartrate is (0.0001 - 0.1) : (0.1 - 5) : (0.001 - 0.5) : (0.003 - 0.5), for example, 1:100:1:3.

[0024] In some embodiments, the hair-fixing and anti-hair loss composition further comprises a plant extract other than Platycladus orientalis leaf extract, and the plant extract is preferably selected from one or more of Magnolia officinalis extract, Polygonum multiflorum extract, Zingiber officinale extract, Salvia miltiorrhiza extract, and Coffea arabica extract.

[0025] In the present invention, the Magnolia officinalis extract can be obtained by extracting from the dried root bark, stem bark or branch bark of Magnolia officinalis of the genus Magnolia in the family Magnoliaceae. The extraction method can be conventional in the art.

[0026] In the present invention, the Polygonum multiflorum extract can be obtained by extracting from the dried tuberous root of Polygonum multiflorum of the family Polygonaceae. The extraction method can be conventional in the art.

[0027] In the present invention, the Salvia miltiorrhiza extract can be obtained by extracting from the dried root and rhizome of Salvia miltiorrhiza of the family Lamiaceae. The extraction method can be conventional in the art.

[0028] In the present invention, the Coffea arabica extract can be obtained by extracting from the coffee fruit or coffee seed of the genus Coffea in the family Rubiaceae. The extraction method can be conventional in the art.

[0029] In some embodiments, the hair-fixing and anti-hair loss composition further comprises a peptide substance, and the peptide substance is preferably selected from one or more of myristoyl pentapeptide-4, myristoyl pentapeptide-17, myristoyl tetrapeptide-12, myristoyl hexapeptide-16, acetyl tetrapeptide-3, oligopeptide-41, oligopeptide-74, copper acetylated peptide, biotin tripeptide-1, acetyl octapeptide-2, tripeptide-1 copper, oligopeptide-1, and tripeptide-34 copper.

[0030] In the present invention, the myristoyl pentapeptide-4 can be obtained by chemical synthesis or genetic engineering methods, and its chemical formula is C 37 H 71 N 7 O 10 , with a molecular weight of 774.00 and a CAS No. of 1392416-25-9.

[0031] In the present invention, the acetyl tetrapeptide-3 can be obtained by chemical synthesis or genetic engineering methods, and its chemical formula is C 22 H 39 N 9 O 5 , with a molecular weight of 509.6 and a CAS No. of 827306-88-7.

[0032] In the present invention, the tripeptide-1 copper, also known as copper peptide, tripeptide, blue copper peptide, and copper peptide, can be obtained by chemical synthesis or genetic engineering methods, and its chemical formula is C 14H 23 CuN 6 O 4 + , with a molecular weight of 402.92.

[0033] In some embodiments, the hair-fixing and anti-hair loss composition further comprises niacinamide.

[0034] In some embodiments, the hair-fixing and anti-hair loss composition further comprises one or more of adenosine substances, plant extracts other than Platycladus orientalis extract, peptide substances, and niacinamide.

[0035] In some embodiments, the hair-fixing and anti-hair loss composition further comprises adenosine substances and plant extracts other than Platycladus orientalis extract.

[0036] In some embodiments, the hair-fixing and anti-hair loss composition comprises type XVII collagen, Platycladus orientalis extract, adenosine, L-carnitine tartrate, and Magnolia officinalis extract.

[0037] In some embodiments, the hair-fixing and anti-hair loss composition consists of type XVII collagen, Platycladus orientalis extract, adenosine, L-carnitine tartrate, and Magnolia officinalis extract.

[0038] In the above embodiments, preferably, the mass ratio of type XVII collagen, Platycladus orientalis extract, adenosine, L-carnitine tartrate, and Magnolia officinalis extract is (0.0001 - 0.1) : (0.1 - 5) : (0.001 - 0.5) : (0.003 - 0.5) : (0.1 - 2), for example, 1:100:1:3:100.

[0039] In some embodiments, the hair-fixing and anti-hair loss composition further comprises adenosine substances and peptide substances. Among them, the peptide substances are preferably myristoyl pentapeptide-4 and / or acetyl tetrapeptide-3.

[0040] In some embodiments, the hair-fixing and anti-hair loss composition comprises type XVII collagen, Platycladus orientalis extract, adenosine, L-carnitine tartrate, and myristoyl pentapeptide-4.

[0041] In some embodiments, the hair-fixing and anti-hair loss composition consists of type XVII collagen, Platycladus orientalis extract, adenosine, L-carnitine tartrate, and myristoyl pentapeptide-4.

[0042] In the above embodiments, preferably, the mass ratio of type XVII collagen, Platycladus orientalis extract, adenosine, L-carnitine tartrate, and myristoyl pentapeptide-4 is (0.0001 - 0.1) : (0.1 - 5) : (0.001 - 0.5) : (0.003 - 0.5) : (0.001 - 3), for example, 1:100:1:3:1.

[0043] In some embodiments, the hair-fixing and anti-hair loss composition comprises collagen type XVII, Platycladus orientalis leaf extract, adenosine, L-carnitine tartrate, and acetyl tetrapeptide-3.

[0044] In some embodiments, the hair-fixing and anti-hair loss composition consists of collagen type XVII, Platycladus orientalis leaf extract, adenosine, L-carnitine tartrate, and acetyl tetrapeptide-3.

[0045] In the above embodiments, preferably, the mass ratio of collagen type XVII, Platycladus orientalis leaf extract, adenosine, L-carnitine tartrate, and acetyl tetrapeptide-3 is (0.0001-0.1) : (0.1-5) : (0.001-0.5) : (0.003-0.5) : (0.003-0.5), for example, 1:100:1:3:3.

[0046] In some embodiments, the hair-fixing and anti-hair loss composition further comprises adenosine substances, peptide substances, and plant extracts other than Platycladus orientalis leaf extract. Among them, the peptide substance is preferably myristoyl pentapeptide-4. Among them, the plant extract other than Platycladus orientalis leaf extract is preferably Magnolia officinalis extract.

[0047] In some embodiments, the hair-fixing and anti-hair loss composition comprises collagen type XVII, Platycladus orientalis leaf extract, adenosine, L-carnitine tartrate, Magnolia officinalis extract, and myristoyl pentapeptide-4.

[0048] In some embodiments, the hair-fixing and anti-hair loss composition consists of collagen type XVII, Platycladus orientalis leaf extract, adenosine, L-carnitine tartrate, Magnolia officinalis extract, and myristoyl pentapeptide-4.

[0049] In the above embodiments, preferably, the mass ratio of collagen type XVII, Platycladus orientalis leaf extract, adenosine, L-carnitine tartrate, Magnolia officinalis extract, and myristoyl pentapeptide-4 is (0.0001-0.1) : (0.1-5) : (0.001-0.5) : (0.003-0.5) : (0.1-2) : (0.001-3), for example, 1:100:1:3:100:1.

[0050] In some embodiments, the hair-fixing and anti-hair loss composition further comprises adenosine substances, peptide substances, plant extracts other than Platycladus orientalis leaf extract, and niacinamide. Among them, the peptide substance is preferably one or more of myristoyl pentapeptide-4, acetyl tetrapeptide-3, and tripeptide-1 copper. Among them, the plant extract other than Platycladus orientalis leaf extract is preferably one or more of Magnolia officinalis extract, Polygonum multiflorum extract, Zingiber officinale extract, Salvia miltiorrhiza extract, and Coffea arabica extract.

[0051] In some embodiments, the hair strengthening and anti - hair loss composition comprises collagen type XVII, Platycladus orientalis leaf extract, adenosine, L - carnitine tartrate, coffee extract, tripeptide - 1 copper, and niacinamide.

[0052] In some embodiments, the hair strengthening and anti - hair loss composition consists of collagen type XVII, Platycladus orientalis leaf extract, adenosine, L - carnitine tartrate, coffee extract, tripeptide - 1 copper, and niacinamide.

[0053] In the above - mentioned embodiments, preferably, the mass ratio of collagen type XVII, Platycladus orientalis leaf extract, adenosine, L - carnitine tartrate, coffee extract, tripeptide - 1 copper, and niacinamide is (0.0001 - 0.1):(0.1 - 5):(0.001 - 0.5):(0.003 - 0.5):(0.0025 - 5):(0.001 - 0.5):(0.05 - 5), for example, 1:100:1:3:2.5:10:500.

[0054] In some embodiments, the hair strengthening and anti - hair loss composition comprises collagen type XVII, adenosine, L - carnitine tartrate, Platycladus orientalis leaf extract, Magnolia officinalis extract, myristoyl pentapeptide - 4, acetyl tetrapeptide - 3, Polygonum multiflorum Thunb. extract, Zingiber officinale extract, Salvia miltiorrhiza extract, coffee extract, tripeptide - 1 copper, and niacinamide.

[0055] In some embodiments, the hair strengthening and anti - hair loss composition consists of collagen type XVII, adenosine, L - carnitine tartrate, Platycladus orientalis leaf extract, Magnolia officinalis extract, myristoyl pentapeptide - 4, acetyl tetrapeptide - 3, Polygonum multiflorum Thunb. extract, Zingiber officinale extract, Salvia miltiorrhiza extract, coffee extract, tripeptide - 1 copper, and niacinamide.

[0056] In the above - mentioned embodiments, preferably, the mass ratio of collagen type XVII, adenosine, L - carnitine tartrate, Platycladus orientalis leaf extract, Magnolia officinalis extract, myristoyl pentapeptide - 4, acetyl tetrapeptide - 3, Polygonum multiflorum Thunb. extract, Zingiber officinale extract, Salvia miltiorrhiza extract, coffee extract, tripeptide - 1 copper, and niacinamide is (0.0001 - 0.1):(0.001 - 0.5):(0.003 - 0.5):(0.1 - 5):(0.1 - 2):(0.001 - 3):(0.003 - 0.5):(0.1 - 10):(0.1 - 10):(0.1 - 6):(0.0025 - 5):(0.001 - 0.5):(0.05 - 5), for example, 1:1:3:100:100:1:3:100:100:100:2.5:10:500.

[0057] In a second aspect, the present invention provides a hair strengthening and anti - hair loss product, which comprises the hair strengthening and anti - hair loss composition as described above.

[0058] In some embodiments, the hair-fixing and anti-hair loss product is shampoo, hair conditioner, hair essence, body wash or body lotion.

[0059] In a third aspect, the present invention provides the use of the hair-fixing and anti-hair loss composition as described above in the preparation of a hair-fixing and anti-hair loss product.

[0060] On the basis of conforming to the common knowledge in the art, the above preferred conditions can be combined arbitrarily to obtain various preferred examples of the present invention.

[0061] The reagents and raw materials used in the present invention are all commercially available.

[0062] The positive and progressive effects of the present invention are as follows:

[0063] 1. The present invention uses type XVII collagen, Platycladus orientalis leaf extract and carnitine substances in combination. On the basis of inhibiting 5α-reductase and antioxidation, it also exerts the effects of hair fixation and anti-hair loss through activating dermal papilla cells, maintaining the health of dermal papilla cells and reducing follicular senescent cells. In addition, the composition of the present invention also has the effect of reducing inflammatory response.

[0064] 2. The preferred composition of the present invention combines one or more of other plant extracts, adenosine substances and peptide substances, which not only exerts the characteristics of comprehensive regulation of traditional Chinese medicine, mildness, safety, overall regulation, equal emphasis on treating the symptoms and root causes, and multi-target treatment, but also exerts the characteristics of high efficiency, stability and high bioavailability of bioactive substances such as adenosine and polypeptides, and exerts the effects of anti-hair loss, hair fixation and hair nourishment from multiple perspectives.

[0065] 3. The composition of the present invention is easy to obtain materials, safe, healthy and stable in efficacy, and is suitable for large-scale industrial production. BRIEF DESCRIPTION OF THE DRAWINGS

[0066] Figure 1 It is a schematic diagram of the test results of the 5α-reductase inhibition rate test of different compositions in each example.

[0067] Figure 2 It is a schematic diagram of the test results of the antioxidant capacity test of different compositions in each example.

[0068] Figure 3 It is a schematic diagram of the test results of the ability to promote COL17 expression of different compositions in each example.

[0069] Figure 4 It is a schematic diagram of the test results of the ability to promote TGF-β2 expression of different compositions in each example.

[0070] Figure 5 It is a schematic diagram of the test results of the ability to promote VEGFA expression of different compositions in each example.

[0071] Figure 6 Schematic diagram of the test results for the ability of different compositions in each example to reduce follicular senescent cells.

[0072] Figure 7 For the hair score results in Application Example 1.

[0073] Figure 8 For the measurement results of the length of the newly grown hair in the depilated area of the mice in Application Example 1.

[0074] Figure 9 For the measurement results of the weight of the newly grown hair in the depilated area of the mice in Application Example 1. Detailed implementation manners

[0075] The present invention will be further illustrated below by way of examples, but the present invention is not limited to the scope of the described examples. For the experimental methods without specific conditions indicated in the following examples, they are carried out according to conventional methods and conditions, or selected according to the product specifications.

[0076] Raw materials such as type XVII collagen, adenosine, L-carnitine tartrate, Platycladus orientalis leaf extract, Magnolia officinalis extract, myristoyl pentapeptide-4, acetyl tetrapeptide-3, Polygonum multiflorum extract, Zingiber officinale extract, Salvia miltiorrhiza extract, coffee extract, copper tripeptide-1 and niacinamide are all purchased from raw material suppliers.

[0077] The manufacturer and model information of each raw material component are as follows:

[0078]

[0079] Example 1 Preparation of hair-fixing and hair-loss prevention composition 1

[0080] Mix type XVII collagen, Platycladus orientalis leaf extract and L-carnitine tartrate in a mass ratio of 1:100:3 to obtain hair-fixing and hair-loss prevention composition 1.

[0081] For subsequent tests, hair-fixing and hair-loss prevention composition 1 is dissolved in water to prepare an aqueous solution. In the solution, the contents of type XVII collagen, Platycladus orientalis leaf extract and L-carnitine tartrate are 0.001%, 0.1% and 0.003% respectively.

[0082] Example 2 Preparation of hair-fixing and hair-loss prevention composition 2

[0083] Mix type XVII collagen, Platycladus orientalis leaf extract, adenosine, L-carnitine tartrate and Magnolia officinalis extract in a mass ratio of 1:100:1:3:100 to obtain hair-fixing and hair-loss prevention composition 2.

[0084] For subsequent tests, the hair-fixing and anti-hair-loss composition 2 was dissolved in water to prepare an aqueous solution. The contents of type XVII collagen, Platycladus orientalis leaf extract, adenosine, L-carnitine tartrate, and Magnolia officinalis extract in the solution were 0.001%, 0.1%, 0.001%, 0.003%, and 0.1% respectively.

[0085] Example 3 Preparation of the hair-fixing and anti-hair-loss composition 3

[0086] Type XVII collagen, Platycladus orientalis leaf extract, adenosine, L-carnitine tartrate, and myristoyl pentapeptide-4 were mixed in a mass ratio of 1:100:1:3:1 to obtain the hair-fixing and anti-hair-loss composition 3.

[0087] For subsequent tests, the hair-fixing and anti-hair-loss composition 3 was dissolved in water to prepare an aqueous solution. The contents of type XVII collagen, Platycladus orientalis leaf extract, adenosine, L-carnitine tartrate, and myristoyl pentapeptide-4 in the solution were 0.001%, 0.1%, 0.001%, 0.003%, and 0.001% respectively.

[0088] Example 4 Preparation of the hair-fixing and anti-hair-loss composition 4

[0089] Type XVII collagen, Platycladus orientalis leaf extract, adenosine, L-carnitine tartrate, and acetyl tetrapeptide-3 were mixed in a mass ratio of 1:100:1:3:3 to obtain the hair-fixing and anti-hair-loss composition 4.

[0090] For subsequent tests, the hair-fixing and anti-hair-loss composition 4 was dissolved in water to prepare an aqueous solution. The contents of type XVII collagen, Platycladus orientalis leaf extract, adenosine, L-carnitine tartrate, and myristoyl pentapeptide-4 in the solution were 0.001%, 0.1%, 0.001%, 0.003%, and 0.003% respectively.

[0091] Example 5 Preparation of the hair-fixing and anti-hair-loss composition 5

[0092] Type XVII collagen, Platycladus orientalis leaf extract, adenosine, L-carnitine tartrate, Magnolia officinalis extract, and myristoyl pentapeptide-4 were mixed in a mass ratio of 1:100:1:3:100:1 to obtain the hair-fixing and anti-hair-loss composition 5.

[0093] For subsequent tests, the hair-fixing and anti-hair-loss composition 5 was dissolved in water to prepare an aqueous solution. The contents of type XVII collagen, Platycladus orientalis leaf extract, adenosine, L-carnitine tartrate, Magnolia officinalis extract, and myristoyl pentapeptide-4 in the solution were 0.001%, 0.1%, 0.001%, 0.003%, 0.1%, and 0.001% respectively.

[0094] Example 6 Preparation of the hair-fixing and anti-hair-loss composition 6

[0095] Mix type XVII collagen, Platycladus orientalis leaf extract, adenosine, L-carnitine tartrate, coffee extract, copper tripeptide-1, and niacinamide in a mass ratio of 1:100:1:3:2.5:10:500 to obtain the anti-hair loss and hair-strengthening composition 6.

[0096] For subsequent tests, dissolve the anti-hair loss and hair-strengthening composition 6 in water to prepare an aqueous solution, where the contents of type XVII collagen, Platycladus orientalis leaf extract, adenosine, L-carnitine tartrate, Magnolia officinalis extract, and myristoyl pentapeptide-4 in the solution are 0.001%, 0.1%, 0.001%, 0.003%, 0.0025%, 0.01%, and 0.5% respectively.

[0097] Example 7 Preparation of the anti-hair loss and hair-strengthening composition 7

[0098] Mix type XVII collagen, adenosine, L-carnitine tartrate, Platycladus orientalis leaf extract, Magnolia officinalis extract, myristoyl pentapeptide-4, acetyl tetrapeptide-3, Polygonum multiflorum Thunb. extract, Zingiber officinale Roscoe extract, Salvia miltiorrhiza Bunge extract, coffee extract, copper tripeptide-1, and niacinamide in a mass ratio of 2:2:6:200:200:2:6:200:200:200:5:20:1000 to obtain the anti-hair loss and hair-strengthening composition 7.

[0099] For subsequent tests, dissolve the anti-hair loss and hair-strengthening composition 7 in water to prepare an aqueous solution, where the contents of type XVII collagen, adenosine, L-carnitine tartrate, Platycladus orientalis leaf extract, Magnolia officinalis extract, myristoyl pentapeptide-4, acetyl tetrapeptide-3, Polygonum multiflorum Thunb. extract, Zingiber officinale Roscoe extract, Salvia miltiorrhiza Bunge extract, coffee extract, copper tripeptide-1, and niacinamide in the solution are 0.001%, 0.001%, 0.003%, 0.1%, 0.1%, 0.001%, 0.003%, 0.1%, 0.1%, 0.1%, 0.0025%, 0.01%, and 0.5% respectively.

[0100] Comparative Example 1 Preparation of the comparative composition 1

[0101] Mix type XVII collagen, Magnolia officinalis extract, and L-carnitine tartrate in a mass ratio of 1:100:3 to obtain the comparative composition 1.

[0102] For subsequent tests, dissolve the comparative composition 1 in water to prepare an aqueous solution. The contents of type XVII collagen, Magnolia officinalis extract, and L-carnitine tartrate in the solution are 0.001%, 0.1%, and 0.003% respectively.

[0103] Comparative Example 2 Preparation of the comparative composition 2

[0104] Mix type XVII collagen, Polygonum multiflorum Thunb. extract, and L-carnitine tartrate in a mass ratio of 1:100:3 to obtain the comparative composition 2.

[0105] For subsequent tests, Comparative Composition 2 was dissolved in water to prepare an aqueous solution. The contents of type XVII collagen, polygonum multiflorum extract, and L-carnitine tartrate in the solution were 0.001%, 0.1%, and 0.003% respectively.

[0106] Comparative Example 3 Preparation of Comparative Composition 3

[0107] Type XVII collagen, ginger extract, and L-carnitine tartrate were mixed in a mass ratio of 1:100:3 to obtain Comparative Composition 3.

[0108] For subsequent tests, Comparative Composition 3 was dissolved in water to prepare an aqueous solution. The contents of type XVII collagen, ginger extract, and L-carnitine tartrate in the solution were 0.001%, 0.1%, and 0.003% respectively.

[0109] Comparative Example 4 Preparation of Comparative Composition 4

[0110] Type XVII collagen, coffee extract, and L-carnitine tartrate were mixed in a mass ratio of 1:2.5:3 to obtain Comparative Composition 4.

[0111] For subsequent tests, Comparative Composition 4 was dissolved in water to prepare an aqueous solution. The contents of type XVII collagen, coffee extract, and L-carnitine tartrate in the solution were 0.001%, 0.0025%, and 0.003% respectively.

[0112] Comparative Example 5 Preparation of Comparative Composition 5

[0113] Type XVII collagen, calamus extract, and L-carnitine tartrate were mixed in a mass ratio of 1:100 to obtain Comparative Composition 5.

[0114] For subsequent tests, Comparative Composition 5 was dissolved in water to prepare an aqueous solution. The contents of type XVII collagen, calamus extract, and L-carnitine tartrate in the solution were 0.001%, 0.1%, and 0.003% respectively.

[0115] Effect Example 1: 5α-Reductase Inhibition Rate Test of the Composition

[0116] 1. Test Procedure

[0117] (1) Sample tube: Add 1 mL of sample solution, 1 mL of enzyme solution, 1 mL of NADPH solution, and 1 mL of testosterone solution to a test tube, shake gently, take 200 μL with a pipette and put it into a 96-well microplate reader. Each sample was made in triplicate and placed in an enzyme-labeled instrument for detection. The absorbance value was measured at 340 nm, which was the first measured value A样品0 After incubating the sample at 37 °C for 20 minutes, it was placed in an enzyme-linked immunosorbent assay (ELISA) reader for detection. The absorbance was measured at 340 nm, which was the second measured value A. 样品20 。

[0118] (2) Enzyme tube: Add 1 mL of PBS solution, 1 mL of enzyme solution, 1 mL of NADPH solution, and 1 mL of testosterone solution into a test tube, shake gently, and pipette 200 μL into a 96-well ELISA plate. Three parallels were made for each sample and then placed in an ELISA reader for detection. The absorbance was measured at 340 nm, which was the first measured value A. 酶0 After incubating the sample at 37 °C for 20 minutes, it was placed in an ELISA reader for detection. The absorbance was measured at 340 nm, which was the second measured value A. 酶20 ; At the same time, blank and positive control detections were performed.

[0119] (3) The 5α-reductase inhibition rate was calculated according to the following formula:

[0120]

[0121] Each sample was tested three times, and the average value was taken and recorded in Table 1.

[0122] 2. Test results

[0123]

[0124] The experimental results are shown in Table 1 and Figure 1 As shown, compared with components 1-13, Examples 1-7 had a significant improvement in inhibiting 5α-reductase activity; among all the compositions, the composition of Example 7 had the strongest ability to inhibit 5α-reductase activity. Compared with Comparative Examples 1-5, Example 1 had a stronger ability to inhibit 5α-reductase activity. It can be seen that compared with other plant extracts, type 17 collagen and L-carnitine tartrate had a better synergistic effect with Platycladus orientalis leaf extract.

[0125] Effect Example 2: Test on the antioxidant ability of the composition

[0126] 1. Test procedure

[0127] (1) Dermal papilla cell seeding: Cells were seeded into a 6-well plate at an inoculation density of 3×10 5 cells / well, and the 6-well plate was placed in an incubator (37 °C, 5% CO 2 ) and incubated overnight.

[0128] (2) Solution preparation: According to different test groups, test substances with different concentrations were prepared respectively.

[0129] (3) H 2 O 2Stimulation: According to the test grouping, when the cell seeding rate in the 6-well plate reaches about 60%, perform H 2 O 2 stimulation, and the induction time is 1 hour.

[0130] (4) Administration: After the induction time ends, administer drugs according to the test grouping, add 2 mL of sample to each well, and set 3 replicate wells for each group. Incubate in an incubator at 37°C and 5% CO 2 for 23 hours.

[0131] (5) Reactive oxygen species (ROS) detection: After incubation, add 1 mL of 10 μM DCFH-DA probe to each well and incubate in an incubator (37°C, 5% CO 2 ) for 30 minutes; discard the culture medium containing DCFH-DA, wash 3 times with PBS, digest the cells with trypsin (0.25%), wash the cells 1 time with PBS, add a certain amount of fresh PBS, and perform flow cytometry detection.

[0132] (6) The ROS inhibition rate is calculated according to the following formula:

[0133]

[0134] For each sample, take the average value of the test results of 3 replicate wells and record it in Table 2.

[0135] 2. Test results

[0136]

[0137] The experimental results are shown in Table 2 and Figure 2 as follows. After treatment with components 1-13 at the above concentrations, the content of reactive oxygen species (ROS) decreased slightly. After treatment with Examples 1-7 at the above concentrations, the content of reactive oxygen species (ROS) decreased significantly. Among them, Example 7 has the strongest ability to inhibit ROS. Compared with Comparative Examples 1-5, Example 1 has a stronger ability to inhibit ROS. It can be seen that compared with other plant extracts, type 17 collagen and L-carnitine tartrate have a better cooperative effect with Platycladus orientalis extract.

[0138] Effect Example 3: Test on the ability to promote the expression of COL17, TGF-β2 and VEGFA

[0139] 1. Test procedure

[0140] (1) Seed dermal papilla cells at 1.5×10 5 cells per well in a 6-well plate and incubate overnight in a CO 2 incubator (37°C, 5% CO 2 ).

[0141] (2) Administration and stimulation. Administration and stimulation were carried out according to the test grouping, and administration and stimulation were carried out simultaneously. Each group was set with 3 duplicate wells. The BC (blank control) group added 2 mL of culture medium to each well, the NC (negative control) group added 2 mL of culture medium containing H 2 O 2 to each well, the PC (positive control) group added 2 mL of culture medium containing H 2 O 2 and WY14643 to each well, and the sample group added culture medium containing H 2 O 2 and the test sample at the corresponding concentration to each well. After completion, all 6-well plates were transferred to a CO 2 incubator (37 °C, 5% CO 2 ) for incubation for 24 hours.

[0142] (3) After the incubation was completed, the old liquid was aspirated and discarded, washed twice with 1 mL / well of PBS, 1 mL of AG RNAex ProReagent was added to each well, the cells were lysed by pipetting, and the samples were collected. After extracting RNA and reverse transcribing it into cDNA, fluorescence quantitative PCR detection was carried out, and the 2 -△△CT method was used for calculation.

[0143] (4) Calculation of upregulation rate:

[0144]

[0145] For each sample, the average value of the test results of 3 duplicate wells was taken and recorded in Tables 3 - 5.

[0146] 2. Test results

[0147]

[0148] The experimental results are shown in Table 3 and Figure 3 as follows. After treatment with the above concentrations, the upregulation rate of the COL 17 gene expression was 44.23% for Component 1 (type 17 collagen), and 17.31% for Component 2 (extract of Platycladus orientalis leaves); after treatment with the above concentrations, the COL 17 gene expression increased slightly for Components 3 - 5, 7, 8, and 10 - 13, but the upregulation rate did not exceed 10%; after treatment with the above concentrations respectively for Components 6 and 9, the COL 17 gene expression did not increase.

[0149] After treatment with the above concentrations in Examples 1-7, the expression level of the COL 17 gene increased significantly, and the up-regulation rate ranged from 89.74% to 159.62%. Among them, Example 7 had the highest up-regulation rate of the COL 17 gene expression. Compared with Comparative Examples 1-5, Example 1 had a higher up-regulation rate of the COL 17 gene expression. It can be seen that, compared with other plant extracts, type 17 collagen and L-carnitine tartrate have a better cooperative effect with Platycladus orientalis leaf extract.

[0150]

[0151] As shown in Table 4 and Figure 4 as shown, after treatment with the above concentrations, the up-regulation rate of the TGF-β2 gene expression of Component 1 (type 17 collagen) was 144.81%, and the up-regulation rate of the TGF-β2 gene expression of Component 2 (Platycladus orientalis leaf extract) was 38.31%; after treatment with the above concentrations of Components 3-11 and 13, the TGF-β2 gene expression increased slightly, but the up-regulation rate did not exceed 20%; after treatment with the above concentrations of Component 12, the TGF-β2 gene expression did not increase, but decreased slightly.

[0152] After treatment with the above concentrations in Examples 1-7, the expression level of the TGF-β2 gene increased significantly, and the up-regulation rate ranged from 314.9% to 442.21%. Among them, Example 7 had the highest up-regulation rate of the TGF-β2 gene expression. Compared with Comparative Examples 1-5, Example 1 had a higher up-regulation rate of the TGF-β2 gene expression. It can be seen that, compared with other plant extracts, type 17 collagen and L-carnitine tartrate have a better cooperative effect with Platycladus orientalis leaf extract. An increase in TGF-β2 gene expression can promote the transformation of hair follicle stem cells from the quiescent phase to the growth phase, which is more beneficial for hair retention.

[0153]

[0154] As shown in Table 5 and Figure 5 as shown, after treatment with the above concentrations, the up-regulation rate of the VEGFA gene expression of Component 1 (type 17 collagen) was 128.16%, and the up-regulation rate of the VEGFA gene expression of Component 2 (Platycladus orientalis leaf extract) was 63.79%; after treatment with the above concentrations of Components 3-13, the VEGFA gene expression increased slightly, but the up-regulation rate did not exceed 23%.

[0155] After the treatments at the above concentrations in Examples 1 - 7, the expression level of the VEGFA gene increased significantly, with the up - regulation rate ranging from 184.48% to 239.66%. Among them, Example 7 had the highest up - regulation rate of the VEGFA gene expression level. Compared with Comparative Examples 1 - 5, Example 1 had a greater up - regulation rate of the VEGFA gene expression level. It can be seen that, compared with other plant extracts, type XVII collagen and L - carnitine tartrate have a better synergistic effect with Platycladus orientalis leaf extract.

[0156] Effect Example 4: Test for the ability to reduce senescent follicle cells

[0157] 1. Test procedure

[0158] (1) Seed dermal papilla cells at a density of 1.5×10 5 cells per well in a 6 - well plate and incubate overnight in a CO 2 incubator (37 °C, 5% CO 2 ).

[0159] (2) Administration and stimulation. According to the test groups, administer drugs and apply stimulation simultaneously. Each group has 3 replicate wells. The BC (blank control) group adds 2 mL of culture medium to each well, the NC (negative control) group adds 2 mL of culture medium containing H 2 O 2 , the PC (positive control) group adds 2 mL of culture medium containing H 2 O 2 and WY14643, and the sample group adds culture medium containing H 2 O 2 and the test sample at the corresponding concentration. After completion, transfer all 6 - well plates to a CO 2 incubator (37 °C, 5% CO 2 ) and incubate for 24 hours.

[0160] (3) Use a β - galactosidase staining kit to stain the cells after treatment with the test substances, and count the proportion of senescent cells among all cells.

[0161] For each sample, take the average of the test results of 3 replicate wells and record it in Table 6.

[0162] 2. Test results

[0163]

[0164] The experimental results are shown in Table 6 and Figure 6As shown, compared with Components 1-13, Examples 1-7 have significantly improved in inhibiting the senescence of dermal papilla cells; among all the compositions, the composition of Example 7 has the strongest ability to inhibit the senescence of dermal papilla cells. Compared with Comparative Examples 1-5, Example 1 has a stronger ability to inhibit the senescence of dermal papilla cells. It can be seen that compared with other plant extracts, type XVII collagen and Platycladus orientalis extract have a better cooperative effect.

[0165] Application Example 1: Preparation of Hair Care Essence

[0166] According to the formula in Table 7, use the anti-hair loss and hair strengthening composition of Example 7 to prepare the hair care essence. The preparation process is as follows:

[0167] Add raw material water and dipropylene glycol into the main pot respectively, stir evenly, then add type XVII collagen and stir until completely dissolved; first premix the plant extracts (Platycladus orientalis extract, Magnolia officinalis extract, Polygonum multiflorum extract, Zingiber officinale extract, Salvia miltiorrhiza extract and Coffea arabica extract) with a certain amount of water, after premixing evenly, add them into the main pot and stir evenly; then add adenosine, L-carnitine tartrate, myristoyl pentapeptide-4, acetyl tetrapeptide-3, tripeptide-1 copper and niacinamide, and stir evenly; then add dipotassium glycyrrhizinate and VE derivative, and stir evenly; test the pH of the material, and add arginine to adjust the pH to 6-7.

[0168]

[0169] The anti-hair loss and hair strengthening efficacy was tested through mouse experiments. Mice in the telogen phase (skin is pink) were selected for the experiment. Take 18 male SPF-grade C57BL / 6 mice, 6-7 weeks old. After 7 days of adaptive feeding of the mice, they were randomly divided into 3 groups according to body weight, namely the normal control group, the positive control group, and the test article group, with 6 mice in each group. Under the anesthesia of the mice, select an area of about 2 cm×3 cm on their backs as the depilation area, and the long side of this area is parallel to the spine. Use a hair clipper to shave the hair in this area, and further depilate with depilatory cream. After pre-hair removal, the mice in the test article group were treated with the test article (the hair care essence of Application Example 1) by skin application, and the intervention period was 21 days. The mice in the normal control group were smeared with normal saline, and the positive control group was smeared with a commercially available competitor (5% minoxidil). The specific grouping and administration information are shown in Table 8:

[0170]

[0171] The mice in each group were photographed every day before and after the intervention to record the skin and hair color of the depilation area. And the hair growth of the mice was scored on the 3rd, 7th, 10th, 14th, and 21st days before and after the intervention. The hair scoring criteria are shown in Table 9, and the scoring results are as Figure 7 shown.

[0172]

[0173] After the administration was completed, the hair quality and hair length of the newly grown hair in the depilated areas of the mice in each group were measured. After removing the newly grown hair in the depilated area, it was weighed, and the hair quality was recorded per square centimeter of skin. In addition, 50 hairs were randomly selected from each mouse, fixed with transparent tape, and the length of the newly grown hair in the depilated area was measured with a ruler, and the measurement results were recorded. The measurement results are shown in Table 10, Figure 8 and Figure 9 as shown

[0174]

[0175] The test results showed that compared with the normal control group and the positive control group, the mice using the hair care essence of the present invention had faster hair growth. By 14 days, more than 80% of the area had hair growth, and the hair was longer and heavier.

[0176] Although the specific embodiments of the present invention have been described above, those skilled in the art should understand that this is only an example. The protection scope of the present invention is defined by the appended claims. Without departing from the principle and essence of the present invention, those skilled in the art can make various changes or modifications to these embodiments, but these changes and modifications all fall within the protection scope of the present invention.

Claims

1. A hair-fixing and anti-hair loss composition, characterized in that: The hair solidifying and anti-hair loss composition comprises type 17 collagen, plant extracts and carnitine substances, and the plant extracts comprise Platycladus orientalis leaf extracts.

2. The hair-fixing and anti-hair loss composition according to claim 1, characterized in that: The molecular weight of the type 17 collagen is 20-200 kDa, such as 23.8 kDa, 97 kDa, 120 kDa or 180 kDa; And / or, the carnitine substance is selected from one or more of carnitine, carnitine hydrochloride, acetylcarnitine hydrochloride, carnitine tartrate and palmitoylcarnitine; preferably, the carnitine substance is carnitine tartrate.

3. The hair-fixing and anti-hair loss composition according to claim 1, characterized in that: The mass ratio of the type 17 collagen, the Platycladus orientalis leaf extract and the carnitine substance is (0.0001-0.1): (0.1-5): (0.003-0.5), for example, 1:100:

3.

4. The hair-fixing and anti-hair loss composition according to any one of claims 1 to 3, characterized in that: The hair-fixing and anti-hair loss composition further comprises an adenosine substance, and the adenosine substance is preferably selected from one or more of adenosine triphosphate disodium, cyclic adenosine monophosphate, adenosine phosphate, adenosine triphosphate and adenosine; And / or, the plant extract further comprises one or more of Magnolia officinalis extract, Polygonum multiflorum extract, Ginger extract, Salvia miltiorrhiza extract and Coffee extract; And / or, the hair-fixing and anti-hair loss composition further comprises a peptide substance, and the peptide substance is preferably selected from one or more of myristoyl pentapeptide-4, myristoyl pentapeptide-17, myristoyl tetrapeptide-12, myristoyl hexapeptide-16, acetyl tetrapeptide-3, oligopeptide-41, oligopeptide-74, copper acetate peptide, biotin tripeptide-1, acetyl octapeptide-2, tripeptide-1 copper, oligopeptide-1 and tripeptide-34 copper; And / or, the hair solidifying and anti-hair loss composition further comprises niacinamide.

5. The hair-fixing and anti-hair loss composition according to claim 4, characterized in that: The hair solidification and anti-hair loss composition includes type 17 collagen, Platycladus orientalis leaf extract, adenosine and carnitine tartrate; preferably, the mass ratio of type 17 collagen, Platycladus orientalis leaf extract, adenosine and carnitine tartrate is (0.0001-0.1): (0.1-5): (0.001-0.5): (0.003-0.5), for example, 1:100:1:

3.

6. The hair-fixing and anti-hair loss composition according to claim 4, characterized in that: The hair-fixing and anti-hair loss composition comprises type 17 collagen, Platycladus orientalis leaf extract, adenosine, carnitine tartrate and Magnolia officinalis extract; preferably, the mass ratio of the type 17 collagen, Platycladus orientalis leaf extract, adenosine, carnitine tartrate and Magnolia officinalis extract is (0.0001-0.1): (0.1-5): (0.001-0.5): (0.003-0.5): (0.1-2), for example, 1:100:1:3:100; Or, the hair-fixing and anti-hair loss composition comprises type 17 collagen, Platycladus orientalis leaf extract, adenosine, carnitine tartrate and myristoyl pentapeptide-4; preferably, the mass ratio of type 17 collagen, Platycladus orientalis leaf extract, adenosine, carnitine tartrate and myristoyl pentapeptide-4 is (0.0001-0.1): (0.1-5): (0.001-0.5): (0.003-0.5): (0.001-3), for example, 1:100:1:3:1; Or, the hair solidification and anti-hair loss composition includes type 17 collagen, Platycladus orientalis leaf extract, adenosine, carnitine tartrate and acetyl tetrapeptide-3; preferably, the mass ratio of type 17 collagen, Platycladus orientalis leaf extract, adenosine, carnitine tartrate and acetyl tetrapeptide-3 is (0.0001-0.1): (0.1-5): (0.001-0.5): (0.003-0.5): (0.003-0.5), for example, 1:100:1:3:

3.

7. The hair-fixing and anti-hair loss composition according to claim 4, characterized in that: The hair-fixing and anti-hair loss composition comprises type 17 collagen, Platycladus orientalis leaf extract, adenosine, carnitine tartrate, Magnolia officinalis extract and myristoyl pentapeptide-4; preferably, the mass ratio of type 17 collagen, Platycladus orientalis leaf extract, adenosine, carnitine tartrate, Magnolia officinalis extract and myristoyl pentapeptide-4 is (0.0001-0.1): (0.1-5): (0.001-0.5): (0.003-0.5): (0.1-2): (0.001-3), for example, 1:100:1:3:100:1; Or, the hair solidification and anti-hair loss composition includes type 17 collagen, Platycladus orientalis leaf extract, adenosine, carnitine tartrate, coffee extract, tripeptide-1 copper and niacinamide; preferably, the mass ratio of type 17 collagen, Platycladus orientalis leaf extract, adenosine, carnitine tartrate, coffee extract, tripeptide-1 copper and niacinamide is (0.0001-0.1): (0.1-5): (0.001-0.5): (0.003-0.5): (0.0025-5): (0.001-0.5): (0.05-5), for example, 1:100:1:3:2.5:10:

500.

8. The hair-fixing and anti-hair loss composition according to claim 4, characterized in that: The hair-fixing and anti-hair loss composition comprises type 17 collagen, adenosine, carnitine tartrate, Platycladus orientalis leaf extract, Magnolia officinalis extract, myristoyl pentapeptide-4, acetyl tetrapeptide-3, Polygonum multiflorum extract, ginger extract, Salvia miltiorrhiza extract, coffee extract, tripeptide-1 copper and niacinamide; Preferably, the mass ratio of type 17 collagen, adenosine, carnitine tartrate, Platycladus orientalis leaf extract, Magnolia officinalis extract, myristoyl pentapeptide-4, acetyl tetrapeptide-3, Polygonum multiflorum extract, ginger extract, Salvia miltiorrhiza extract, coffee extract, tripeptide-1 copper and niacinamide is (0.0001-0.1): (0.001-0.5): (0.003-0.5): (0.1-5): (0.1-2): (0.001-3): (0.003-0.5): (0.1-10): (0.1-10): (0.1-6): (0.0025-5): (0.001-0.5): (0.05-5), for example, 1:1:3:100:100:1:3:100:100:100:2.5:10:

500.

9. A hair-fixing and anti-hair loss product, comprising the hair-fixing and anti-hair loss composition according to any one of claims 1 to 8; Preferably, the hair solidifying and anti-hair loss product is shampoo, conditioner, hair essence, shower gel or body lotion.

10. Use of the hair-fixing and anti-hair loss composition according to any one of claims 1 to 8 in the preparation of a hair-fixing and anti-hair loss product.

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