Cosmetic composition containing lotus corniculatus extract

Abstract

The present invention relates to a cosmetic composition containing a lotus corniculatus extract, and more particularly, to a cosmetic composition containing a lotus corniculatus extract in which the contents of low molecular weight proteins and tannic acid are increased by a specific extraction method, thereby having an excellent pore-shrinking effect.

Description

Technical Field

[0001] The present invention relates to a cosmetic composition containing an extract of Lotus corniculatus, and more particularly, to a cosmetic composition containing an extract of Lotus corniculatus in which the content of low molecular weight proteins and tannic acid is increased by a specific extraction method, and which has an excellent pore-tightening effect, in particular. Background Art

[0002] Recently, for the cosmetic improvement of the skin, functional cosmetics for preventing or treating skin aging and cosmetic or food materials having the effect of shrinking skin pores are widely consumed by people of all ages, both men and women.

[0003] Pores (hair follicles), as the holes through which hair grows, are also connected to sebaceous glands, so sebum secreted by the sebaceous glands is discharged to the skin surface through the pores. Generally, the diameter of pores is about 0.02 - 0.05 mm, which is very small and usually invisible to the naked eye. However, when sebum secretion is excessive due to age, season, a woman's menstrual cycle, pregnancy, stress, etc., the pores will become large enough to be visually confirmed. Skin with large pores leaves a bad impression in terms of beauty.

[0004] In addition, the sebaceous glands attached to the pores secrete sebum, which protects the skin from external damage and prevents bacterial infection, and functions to keep the skin moist. Sebaceous glands are inactive with only traces before puberty, and then enter puberty when sex hormones in the body are strong, the sebaceous glands become larger and produce a lot of sebum, which is discharged outside the pores. In puberty, diseases such as acne are caused due to the active activity of sebaceous glands. On the contrary, as age increases, the function of sebaceous glands decreases, resulting in dry skin and causing itching, etc. Pores become larger during the puberty period when sebum secretion is excessive. When entering puberty, sebum secretion increases due to hormonal influence, and pores become larger to discharge sebum. In addition, when sebum stays in the pores during the process of discharging outward, the remaining sebum makes the pores even larger. Another reason for enlarged pores can be skin aging. Skin aging affects the modification or reduction of collagen fibers and elastic fibers that support pores. As a result, due to the lack of the force to support pores, pores will naturally become larger.

[0005] When pores become larger, bacteria are likely to invade the hair follicles, easily causing skin problems such as acne. For this reason, more and more people are paying attention to pore tightening, and pore and sebum management has become very important.

[0006] Astringent action is used to shrink enlarged pores and proteins in the skin such as collagen, and to condition the skin texture. It refers to the action of temporarily tightening skin proteins and inhibiting the secretion of excessive sebum or sweat.

[0007] The astringent effect on the skin is divided into two types: chemical astringent effect and physical astringent effect. The chemical astringent effect refers to the situation where sweat glands and pores are temporarily constricted by substances such as tannins that cause protein coagulation. On the contrary, the physical astringent effect refers to the situation where cold water is applied to the skin or the evaporation of ethanol is used to lower the skin temperature, thereby temporarily constricting sweat glands and pores.

[0008] So far, many studies have been conducted on pore-tightening compositions using natural extracts. However, there is still a need to develop natural materials with excellent skin pore-tightening effects.

[0009]

Prior Art Documents

[0010]

Patent Documents

[0011] Republic of Korea Patent Publication No. 2011-0047717

[0012] Republic of Korea Patent Publication No. 2013-0016929 Summary of the Invention

[0013] Technical Problem to be Solved

[0014] For this reason, the present inventors wanted to find natural materials that are safe for the skin and have excellent pore-tightening effects. It was found that tannin components exist in Lotus corniculatus, and when the Lotus corniculatus extract is prepared by a specific method, the contents of low-molecular proteins and tannic acid in the extract increase, thereby being able to show excellent pore-tightening effects. Thus, the present invention was completed.

[0015] Therefore, an object of the present invention is to provide a cosmetic composition containing a Lotus corniculatus extract with excellent pore-tightening effects.

[0016] Means for Solving the Problem

[0017] To achieve the above object, the present invention provides a cosmetic composition containing a Lotus corniculatus extract.

[0018] In addition, the Lotus corniculatus extract according to the present invention is obtained by a method including the following steps:

[0019] (a) A step of pre-treating Lotus corniculatus according to the used part;

[0020] (b) A step of roasting the pre-treated Lotus corniculatus;

[0021] (c) A step of pulverizing the cell wall of the roasted Lotus corniculatus to obtain a primary extract.

[0022] (d) The step of subjecting the proteins contained in the extract to low molecular weight quantification to obtain a secondary extract with an increased content of low molecular weight proteins; and

[0023] (e) The step of stabilizing the secondary extract.

[0024] In addition, the cosmetic according to the present invention provides a pore - shrinking effect.

[0025] In addition, the present invention provides the use of a Lotus corniculatus extract as an active ingredient in the preparation of a cosmetic composition for shrinking skin pores.

[0026] Advantages of the Invention

[0027] The composition of the present invention contains ingredients derived from the source plant as active ingredients, so it is safe for the skin and has an excellent pore - shrinking effect. Brief Description of the Drawings

[0028] Figure 1 Shows the molecular weight distribution confirmed by gel permeation chromatography for the sample of Comparative Example 1.

[0029] Figure 2 Shows the molecular weight distribution confirmed by gel permeation chromatography for the sample of Example 1.

[0030] Figure 3 Shows the protein aggregation effect confirmed by absorbance at 650 nm for Example 6 (100%) and tannic acid (0.5 mg / mL). Detailed Description of the Invention

[0031] The present invention relates to a composition containing a Lotus corniculatus extract.

[0032] Lotus corniculatus L., as a plant of the genus Lotus in the family Leguminosae of the order Rosales, has tannins on its flowers, and it is well - known that the flower extract has effects such as skin whitening and antioxidant properties.

[0033] Tannins generally refer to various polyphenolic compounds with various high-molecular structures and containing sufficient hydroxyl groups and other suitable functional groups for the formation of highly reactive complexes. Tannins are astringent substances that cause the dry and puckery feeling after eating unripe fruits or drinking red wine or tea, and are synthesized by plants. Tannins are polyphenolic biomolecules with astringent properties that bind to and precipitate various organic compounds including proteins, amino acids, and alkaloids, and are known to play a defensive function against pests and diseases in plants. Tannins are classified into gallic acid esters with molecular weights ranging from 500 to over 3000 and proanthocyanidins with a maximum molecular weight of 20,000.

[0034] The composition according to the present invention includes an extract of Lotus corniculatus as an active ingredient.

[0035] In particular, in the present invention, the extract of Lotus corniculatus is obtained by a method for preparing an extract including the following steps:

[0036] (a) A step of pre-treating Lotus corniculatus according to the part used;

[0037] (b) A step of roasting the pre-treated Lotus corniculatus;

[0038] (c) A step of crushing the cell wall of the roasted Lotus corniculatus to obtain a primary extract;

[0039] (d) A step of reducing the molecular weight of the protein contained in the extract to obtain a secondary extract with an increased content of low-molecular-weight protein; and

[0040] (e) A step of stabilizing the secondary extract.

[0041] The content of low-molecular-weight protein in the extract obtained by the above method will increase.

[0042] The part used of Lotus corniculatus may be flowers, fruits, seeds, leaves, stems, roots, or the whole plant, preferably flowers, and the form may be raw or dried, but is not limited thereto.

[0043] In the step (a), the pre-treatment may be one or more of cutting, drying, and crushing of Lotus corniculatus, or removing impurities and parts other than the part used of Lotus corniculatus from raw or dried commercially available Lotus corniculatus.

[0044] The drying can be hot air drying, vacuum drying, freeze drying, etc. The hot air drying or vacuum drying can preferably be completed at a temperature of 32-60°C, more preferably at 40-50°C. The freeze drying can be completed at a temperature below -60°C, but is not limited thereto.

[0045] During the drying, the water content of the Lotus corniculatus, based on the total weight of the plant, is preferably 0.1% by weight or less, but is not limited thereto.

[0046] In the step (b), roasting means dry heating the pretreated Lotus corniculatus at a high temperature, preferably at 80-120°C, more preferably at 90-110°C, and most preferably at 95-105°C for 5-60 minutes, more preferably 7-40 minutes, and most preferably 10-20 minutes, which means heating or stir-frying without adding water, but is not limited thereto. The heating device used for the roasting can be an oven, a gas stove, a microwave oven, etc., but as long as it is a heating device capable of adjusting the temperature, it is not restricted.

[0047] The roasting of the Lotus corniculatus is used to remove the water contained in the plant, and is characterized in that when performing the step (c) to step (d), the content of low molecular weight proteins contained in the Lotus corniculatus is increased.

[0048] The roasting of the Lotus corniculatus is used to remove the water contained in the Lotus corniculatus, and is characterized in that it removes the inherent smell, special smell, stench, etc. of the Lotus corniculatus.

[0049] In the step (c), the extract (primary extract) is obtained by using water, an alcohol having 1 to 4 carbon atoms (such as methanol, ethanol, butanediol), or a mixture of water and an alcohol having 1 to 4 carbon atoms as the extraction solvent. Preferably, the extract can be obtained by using water, ethanol or butanediol as the extraction solvent, more preferably obtained by using water and butanediol. In this case, the extract can also be obtained by sequentially using water and butanediol or butanediol and water. It can be a mixture of an extract obtained by using water (water extract) and an extract obtained by using butanediol (butanediol extract). Even more preferably, it can be a mixture in which the water extract and the butanediol extract are mixed at a weight ratio of 1-20:1-20, preferably 1-5:1-15.

[0050] The roasted Lotus corniculatus and the extraction solvent are mixed at a weight ratio of 1:10-1:50, preferably 1:20.

[0051] The present invention is characterized in that, in the step (c), the comminution of the Lotus japonicus cell wall can be achieved by using one or more enzymes selected from the group (the first enzyme group) consisting of cellulase, pectinase, α,β-glucosidase, amyloglucosidase, β-glucanase, naringinase, α-amylase, hemicellulase, arabinase, xylanase, and tannase.

[0052] The present invention is characterized in that, in the step (c), the enzyme content of the first enzyme group added to the roasted Lotus japonicus for comminuting the Lotus japonicus cell wall is preferably 0.01 - 10% by weight, more preferably 0.03 - 5% by weight, and most preferably 0.05 - 0.3% by weight based on the total weight of the Lotus japonicus (raw material). When the enzyme content is less than 0.01% by weight, the yield of the extract from the roasted Lotus japonicus may decrease. When the enzyme content exceeds 10% by weight, the increase in the yield of the extract from the roasted Lotus japonicus may not be significant compared to the increase in content.

[0053] The step (c) may further include: filtering the product obtained after comminuting the Lotus japonicus cell wall at a mesh size of 50 - 270 mesh, preferably 80 - 230 mesh, more preferably 100 - 200 mesh, and most preferably 140 - 170 mesh.

[0054] The step (c) may further include: heat-treating the product obtained after comminuting the Lotus japonicus cell wall at a temperature of 40 - 100 °C, preferably 50 - 85 °C, and most preferably 75 - 82 °C for 0.1 - 3 hours, preferably 0.5 - 2 hours, and most preferably 1 - 2 hours. This heat-treatment step can inactivate the hydrolase (the first enzyme group).

[0055] The present invention is characterized in that, in the step (d), the low-molecular-weight conversion is to convert the protein extracted by treating one or more enzymes selected from the group (the second enzyme group) consisting of protease / peptidase, preferably exopeptidase and endopeptidase (such as flavourzyme, alcalase, etc.) from a high molecular weight to a low molecular weight, preferably to convert it to a low molecular weight peptide.

[0056] The present invention is characterized in that, in the step (d), the enzyme content of the second enzyme group added to the extract to reduce the molecular weight of the protein contained in the extract is preferably 0.01-10% by weight, more preferably 0.03-5% by weight, and most preferably 0.05-0.3% by weight based on the total weight of the roasted Lotus corniculatus. When the enzyme content is less than 0.01% by weight, the content of low-molecular-weight proteins included in the extract of the roasted plant may decrease. When the enzyme content exceeds 10% by weight, the increase in the content of low-molecular-weight proteins included in the extract of the roasted plant may not be significant compared to the increase in content.

[0057] The present invention is characterized in that the high-molecular-weight protein has a size of 5500 Da or more, preferably 10000-60000 Da, and more preferably 20000-50000 Da, and the low-molecular-weight protein or peptide has a size of 100-5000 Da, preferably 200-4000 Da, and more preferably 300-2000 Da. When the size of the low-molecular-weight protein or peptide is less than 100, there may be almost no effective physiological effects, such as pore-tightening effects, etc. When the size of the low-molecular-weight protein exceeds 5000 Da, the increase in the effective physiological effects, etc. may not be significant compared to the increase in size.

[0058] The present invention is characterized in that, in the step (e), stabilization is achieved by subjecting the extract (secondary extract) containing the low-molecular-weight protein to high-temperature treatment for sterilization / sterilization and inactivating the hydrolases (such as the second enzyme group) that are originally contained in the extract or may be included in the extraction process. This is useful for maintaining the activity and long-term preservation of the extract containing the low-molecular-weight protein.

[0059] The present invention is characterized in that, in the step (e), stabilization is achieved by rapidly raising the temperature of the low-molecular-weight protein, preferably the low-molecular-weight peptide, to 75 °C or higher, preferably 85 °C or higher, and most preferably 85-90 °C, and heating for a certain period of time, preferably 10-180 minutes, more preferably 30-120 minutes, and most preferably 60-90 minutes.

[0060] In the step (e), the stabilization step may further include: after subjecting the low-molecular-weight protein, preferably the low-molecular-weight peptide, to the heating treatment, filtering it using a microfilter having a pore size of 0.1-8.0 μm, preferably 0.2-5.0 μm, and most preferably 0.5-0.8 μm.

[0061] In the composition of the present invention, the extract of Lotus corniculatus can be contained in an amount of 0.01 - 70% by weight, preferably 0.1 - 30% by weight, more preferably 0.1 - 20% by weight, based on the total weight of the composition.

[0062] The composition according to the present invention shows anti-aging (improving wrinkles, increasing elasticity) effects along with antioxidant effects by increasing the content of low-molecular-weight proteins. In addition, the content of tannic acid increases, thereby showing excellent effects such as shrinking (astringing) pores and controlling sebum, and is particularly effective for shrinking (astringing) pores.

[0063] The composition according to the present invention can be formulated into various dosage forms such as a cosmetic composition, a pharmaceutical composition, etc.

[0064] The composition according to the present invention can be a cosmetic composition. For example, it can be a base cosmetic, a color cosmetic, a body care cosmetic, etc., and is not specifically limited thereto. It can be formulated into a flexible lotion, an astringent lotion, a milk, a cream, a massage cream, a serum, an eye cream, an eye serum, a cleansing cream, a cleansing foam, a makeup remover, a mask, a body milk, a body cream, a body oil, a body serum, etc., and can be appropriately selected according to the purpose.

[0065] Considering the usage limits based on the rules related to cosmetic safety, the cosmetic composition can also contain auxiliaries commonly used in the fields of cosmetics or dermatology such as humectants (polyols, etc.), oils, waxes, viscosity regulators, thickeners, pH adjusters (pH regulators), fats, organic solvents, solubilizers, concentrates, gelling agents, softeners, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, water, ionic emulsifiers, non-ionic emulsifiers, fillers, sequestering agents, chelating agents, preservatives, vitamins, wetting agents, dyes, pigments, hydrophilic activators, lipophilic activators, lipid vesicles, or any other components commonly used in cosmetics. In addition, the composition of the present invention can also contain skin penetration enhancers to enhance the effect of improving skin condition.

[0066] As a pharmaceutical composition, the composition can be in dosage forms such as tablets, ointments, milks, gels, creams, sprays, suspensions, oils, patches, etc., but is not limited thereto.

[0067] Hereinafter, the present invention will be further described in detail by way of examples. Those with ordinary knowledge in the art should clearly understand that these examples are only for exemplifying the present invention, and the scope of the present invention is not limited by these examples.

[0068]

Example 1

[0069] First, the flowers of Lotus japonicus were pretreated by cutting, drying, and pulverizing in sequence to obtain the pretreated crude material. The pretreated crude material was baked (high-temperature dry heat) at about 100 °C for 20 minutes to obtain the baked crude material. After mixing the baked crude material and distilled water at a weight ratio of 1:30, cellulase was added at 0.1% by weight based on the total weight of the crude material and treated at about 50 °C for 2 hours. After pulverizing the cell wall of the baked crude material, filtration was performed using a 150-mesh sieve to obtain a filtrate. The filtrate was heat-treated at a temperature of about 80 °C for 1 hour to obtain a filtrate with inactivated cellulase (storage temperature: about 4 - 7 °C). Two kinds of proteases, Alcalase 2.4L FG (Novozymes) and Flavourzyme 1000L (Novozymes), were added to the filtrate with inactivated cellulase. Each of Alcalase and Flavourzyme was added at 0.1% by weight based on the total weight of the baked plant, and then treated at about 50 °C for 2 hours to hydrolyze the high-molecular-weight proteins contained in the filtrate with inactivated cellulase into low-molecular-weight proteins. To inactivate the proteases included in the filtrate, after heat-treatment at about 80 °C for 1 hour, the temperature was lowered to about 4 - 7 °C, and then filtration was performed using a filter with a pore size of 0.8 μm (0.8 μm pore size filter). The filtrate thus obtained was used as the final product.

[0070]

Example 2

[0071] The flowers of Lotus japonicus were pretreated in the same manner as in Example 1. The crude material was extracted with 99% ethanol by stirring at 80 °C at 250 - 500 rpm for 2 hours. After extraction, ethanol was removed using a vacuum concentrator, and filtration was performed using a filter with a pore size of 0.8 μm. The filtrate thus obtained was used as the final product.

[0072]

Example 3

[0073] The flowers of Lotus japonicus were pretreated in the same manner as in Example 1. The crude material was extracted with 70% ethanol by stirring at 80 °C at 250 - 500 rpm for 2 hours. After extraction, ethanol was removed using a vacuum concentrator, and filtration was performed using a filter with a pore size of 0.8 μm. The filtrate thus obtained was used as the final product.

[0074]

Example 4

[0075] The pretreatment of the flowers of Lotus corniculatus was carried out by the same method as in Example 1. The original material was extracted by stirring with hot water at 80 °C at 250 - 500 rpm for 2 hours. It was filtered using a filter with a pore size of 0.8 μm, and the resulting filtrate was used as the final product.

[0076]

Example 5

[0077] The pretreatment of the flowers of Lotus corniculatus was carried out by the same method as in Example 1. The original material was extracted by stirring with 50% butylene glycol (BG) at 80 °C at 250 - 500 rpm for 2 hours. It was filtered using a filter with a pore size of 0.8 μm, and the resulting filtrate was used as the final product.

[0078]

Example 6

[0079] The extracts prepared in Example 1 and Example 5 were each added to pure water at 5% and 15% relative to the total weight of the mixture, thereby preparing the mixture.

[0080]

Comparative Example 1

[0081] First, the flowers of Lotus corniculatus were pretreated by cutting, drying, and pulverizing in sequence to obtain the pretreated original material. After mixing the pretreated original material and distilled water at a weight ratio of 1:30, it was left standing at about 50 °C for 2 hours, and then filtered using a 150 - mesh sieve, thereby obtaining the filtrate.

[0082]

Comparative Example 2

[0083] The pretreatment of the flowers of Lotus corniculatus was carried out by the same method as in Comparative Example 1. The original material was extracted by stirring with 99% ethanol at 80 °C at 250 - 500 rpm for 2 hours. After extraction, ethanol was removed using a vacuum concentrator, and it was filtered using a filter with a pore size of 0.8 μm, and the resulting filtrate was used as the final product.

[0084]

Comparative Example 3

[0085] The pretreatment of the flowers of Lotus corniculatus was carried out by the same method as in Comparative Example 1. The original material was extracted by stirring with 70% ethanol at 80 °C at 250 - 500 rpm for 2 hours. After extraction, ethanol was removed using a vacuum concentrator, and it was filtered using a filter with a pore size of 0.8 μm, and the resulting filtrate was used as the final product.

[0086]

Comparative Example 4

[0087] The flowers of Lotus corniculatus were pretreated in the same manner as in Comparative Example 1. The original material was extracted by stirring with hot water at 80 °C at 250 - 500 rpm for 2 hours. Filtration was carried out using a filter with a pore size of 0.8 μm, and the resulting filtrate was used as the final product.

[0088]

Comparative Example 5

[0089] The flowers of Lotus corniculatus were pretreated in the same manner as in Comparative Example 1. The original material was extracted by stirring with 50% butylene glycol (BG) at 80 °C at 250 - 500 rpm for 2 hours. Filtration was carried out using a filter with a pore size of 0.8 μm, and the resulting filtrate was used as the final product.

[0090]

Comparative Example 6

[0091] The extracts prepared in Comparative Example 1 and Comparative Example 5 were each added to pure water at 5% and 15% relative to the total weight of the mixture to prepare the mixture.

[0092]

Test Example 1

[0093] 1. Determination of protein content in Lotus corniculatus extract

[0094] The samples prepared in Examples 1 to 5 and Comparative Examples 1 to 5 were analyzed for protein content, components of each structure, and molecular weight.

[0095] 1) The protein content in each sample (extract) was confirmed using a protein kit (Lowry protein assay) (each sample was measured 3 times and the average value was taken) (reference: LOWRY, O H et al., J Biol Chem., 193(1):265 - 75, 1951). The results are shown in Table 1 below.

[0096]

Table 1

[0097] Distinction Polypeptide (protein) content (ppm) Example 1 6400 Example 2 3100 Example 3 3346 Example 4 5300 Example 5 3940 Comparative Example 1 3420 Comparative Example 2 2480 Comparative Example 3 2800 Comparative Example 4 4700 Comparative Example 5 3647

[0098] Observing Table 1, it can be confirmed that in Examples 1 to 5 as extracts obtained by the method according to the present invention, the polypeptide (protein) content is significantly increased compared to Comparative Examples 1 to 5. In particular, in Example 1 where distilled water was used as the solvent, the polypeptide content almost doubled.

[0099] 2. Determination of tannic acid content

[0100] Dissolve tannic acid purchased from SIGMA-ALDRICH in 10 mL of distilled water, dilute it to concentrations of 2000, 1000, 500, 250, 125, and 62.5 ppm for preparation, and use them as tannic acid standards.

[0101] Take out 1 mL of each diluted tannic acid standard and the samples of Examples 1 to 5 and Comparative Examples 1 to 5 respectively. Add 1 mL of 99.5% ethanol and 1 mL of distilled water to the taken-out 1 mL of tannic acid standard and the samples, and mix them by vortexing for 30 seconds. Add 1 mL of 5% Na 2 CO 3 and 0.5 mL of 1N Folin-Ciocalteu phenol reagent, mix them by vortexing, and after color development in a dark room at room temperature for 60 minutes, place them in a 96-well plate, and measure the absorbance at 725 nm using a microplate reader (UVT-06685, Thermo max, USA). The measurement results are shown in Table 2 below.

[0102]

Table 2

[0103] Distinction Tannic acid content (ppm) Example 1 950 Example 2 2750 Example 3 2540 Example 4 640 Example 5 3300 Comparative Example 1 360 Comparative Example 2 1900 Comparative Example 3 1640 Comparative Example 4 427 Comparative Example 5 2480

[0104] Observing Table 2, it can be confirmed that for the extracts obtained by the method according to the present invention in Examples 1 to 5, the tannic acid content is significantly increased compared to Comparative Examples 1 to 5 respectively.

[0105] 3. Determination of molecular weight

[0106] To confirm the molecular weights of the substances included in the samples of Example 1 and Comparative Example 1 according to the present invention, gel permeation chromatography (GPC) is performed. Specifically, using a pL aqua-gel OH-30, 60, Mixed-M (7.5X 300 mm, Agilent, USA) chromatographic column and high-pressure liquid chromatography (Agilent Technologies 1200 Series HPLC / GPC, Agilent, USA), RI detector (Agilent, USA), detect under the conditions of mobile phase distilled water, column temperature 35°C, flow rate of 1 mL per minute, and detector temperature 35°C. Use polysaccharides of each molecular weight as standard substances, write a calibration curve of molecular weight based on the retention time of each sample in the column, and confirm the molecular weight distribution (measure 3 for each sample and take the average). Figure 1 (Comparative Example 1) andFigure 2 The results are shown in (Example 1).

[0107] It was confirmed that in Comparative Example 1, as Figure 1 shown, about 9% showed an average molecular weight of about 280,000 Da, about 8.2% showed an average molecular weight of about 38,000 Da, and the remaining 82.8% showed a molecular weight of 500 Da or less. In Example 1, as Figure 2 shown, about 7% showed an average molecular weight of about 280,000 Da, about 5.3% showed an average molecular weight of about 16,000 Da, and the remaining 87.7% showed a molecular weight of 500 Da or less. That is, compared with Comparative Example 1, the overall protein molecular weight in Example 1 prepared according to the present invention is smaller.

[0108]

Test Example 2

[0109] Based on the results of Test Example 1, Example 6 was prepared by mixing Example 1 with the highest polypeptide (protein) content and Example 5 with the highest tannic acid content, and the protein aggregation effect of Example 6 was measured.

[0110] When the protein components around the pores aggregate, the pores become tighter, so it can contribute to pore tightening. Therefore, the higher the absorbance value, the higher the protein aggregation effect, and the better the protein aggregation effect, indicating a significant pore-tightening effect.

[0111] To measure the protein aggregation effect on the Lotus japonicus extract, 1 mL of the above-obtained Lotus japonicus extract (Example 6) was added to 2 mL of a 50 mM citric acid buffer solution (pH 4.5) containing 0.3% albumin as a protein, shaken well, allowed to stand for 5 minutes, and then the absorbance was measured at 650 nm (Microplate reader, BioTeK) to determine the turbidity. Figure 3 The results are shown in. In addition, for comparison, the protein aggregation effect on tannic acid was measured. Except that tannic acid was added to the citric acid buffer solution at a concentration of 0.5 mg / mL, the same method as the method for measuring the protein aggregation effect on the Lotus japonicus extract was carried out. The measurement results are shown in Table 3 below and Figure 3 The measurement results are shown in.

[0112]

Table 3

[0113] Distinction Absorbance OD of protein aggregation ability Example 1 0.012 Example 5 0.077 Example 6 0.031 Comparative Example 6 0.022 Tannic acid (0.5mg / mL) 0.051

[0114] Observing Table 3 and Figure 3 , Example 5 with a high tannic acid content was determined to have the highest absorbance of protein aggregation ability, and it also showed a higher efficacy compared to the case of treating 0.5 mg / mL of tannic acid.

[0115] In addition, the Lotus corniculatus extract prepared according to the present invention (Example 6) shows excellent protein agglutination ability compared to the extract prepared by the ordinary extraction method (Comparative Example 6), and thus it can be confirmed that it can effectively act on pore tightening.

[0116] Based on the comprehensive test results, it is evaluated that in Example 5, the tannic acid content is high and the protein agglutination ability is also high. However, when actually applied to the skin, the low-molecular-weight extract is easily penetrated into the skin. Therefore, even if the tannic acid content is high, the pore shrinking effect will not be so high. Therefore, when actually applying Example 6, which mixes Example 1 with a high content of low-molecular-weight protein and Example 5 with a high tannic acid content, to the skin, a more preferable effect can be obtained.

Claims

1. A cosmetic composition for shrinking skin pores, comprising a Lotus japonicus extract.

2. The cosmetic composition according to claim 1, wherein The Lotus japonicus extract is obtained by a method comprising the following steps: (a) pre-treating the lotus root according to the part to be used; (b) the step of baking the pretreated Lotus japonicus; (c) crushing the cell walls of the roasted Lotus japonicus to obtain a primary extract; (d) a step of reducing the molecular weight of the protein contained in the extract to obtain a secondary extract having an increased content of low molecular weight protein; and (e) a step of stabilizing the secondary extract.

3. The cosmetic composition according to claim 2, wherein The primary extract is obtained by using one or more of water, ethanol and butanediol as an extraction solvent.

4. The cosmetic composition according to claim 3, wherein The primary extract is obtained by using water and butanediol as extraction solvents.

5. The cosmetic composition according to claim 1, wherein The Lotus japonicus extract is contained in an amount of 0.1-20 weight % relative to the total weight of the composition.

6. Use of a Lotus japonicus extract as an effective ingredient in preparing a cosmetic composition for shrinking skin pores.

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