Soothing and repairing composition and application thereof

Abstract

The invention relates to the technical field of daily chemicals, in particular to a soothing and repairing composition and application thereof. The composition comprises a scutellaria baicalensis root extract and a leontopodium alpinum extract. According to the invention, a solid aerobic fermentation method is utilized, and different plant attributes of substrates are researched; screening and combining are conducted from a strain library, the most appropriate combination (eurotium cristatum compound or eurotium cristatum and other strains) is selected, and active matter indexes are directionally improved; on the basis of multiple enzymolysis of the compound strain and generation of secondary metabolites, active ingredients are enriched, and more small-molecule polypeptides are obtained.

Description

Technical Field

[0001] The present invention relates to the technical field of daily chemical products, and particularly relates to a soothing and repairing composition and its application. Background Art

[0002] Sensitive skin (sensitive skin type) refers to a skin condition that is very sensitive to external stimuli and is prone to discomfort or allergic reactions, rather than a specific skin type. Sensitive skin usually shows symptoms such as dry skin, stinging, redness, itching, flushing, peeling, etc., and is more sensitive to environmental changes, cosmetic ingredients, and climate changes.

[0003] Main Characteristics of Sensitive Skin

[0004] 1. Impaired skin barrier function: The natural barrier of the skin is weak, moisture loss is fast, and it is easily affected by external stimuli.

[0005] 2. Sensitive to external stimuli: Prone to be affected by climate, temperature, cosmetics, pollution, stress, etc.

[0006] 3. Prone to flushing or inflammation: Especially after the alternation of hot and cold or being stimulated, the skin is prone to flushing.

[0007] 4. Prone to dryness or stinging: The skin is thinner, sebum secretion is insufficient, resulting in a feeling of dryness, tightness or stinging.

[0008] When choosing skin care products for sensitive skin, special attention needs to be paid to the safety and mildness of the ingredients, and ingredients that may cause irritation should be avoided. Selection of skin care products for sensitive skin: 1. Simplified ingredients: Choose skin care products with fewer and clear ingredients and avoid complex formulas. 2. No irritating additives: Try to avoid containing alcohol, spices, preservatives, essential oils or other ingredients that may cause sensitivity. 3. Slightly acidic products: Products close to the natural pH value of the skin (about 5.5), which are mild and do not damage the skin barrier. 4. Targeted at repairing the barrier: Prioritize products containing repair ingredients such as ceramides, cholesterol, hyaluronic acid, panthenol (vitamin B5), etc. 5. Special for sensitive skin: Choose skin care products marked "suitable for sensitive skin" or "low-sensitivity formula".

[0009] Eurotium cristatum is a fungus belonging to the genus Eurotium of the family Aspergillaceae, and is named after its unique coronal conidial head morphology. This kind of fungus mainly exists in the natural environment and is also widely used in food processing due to its fermentation characteristics.

[0010] The metabolites produced by Eurotium cristatum during the fermentation process have certain antioxidant, intestinal microbiota regulation, blood lipid lowering and immune regulation effects. Eurotium cristatum is a model of the combination of traditional fermentation technology and microbiology.

[0011] To this end, the present invention provides a soothing and repairing composition and its application. Summary of the Invention

[0012] To solve the above technical problems, the present invention provides a soothing and repairing composition, which comprises: Scutellaria baicalensis root extract, Leontopodium alpinum extract.

[0013] As an embodiment of the present invention, the mass ratio of the Scutellaria baicalensis root extract to the Leontopodium alpinum extract is 3-7:2-8.

[0014] As an embodiment of the present invention, the mass ratio of the Scutellaria baicalensis root extract to the Leontopodium alpinum extract is 5:4.

[0015] As an embodiment of the present invention, the composition further comprises citrus peel extract.

[0016] As an embodiment of the present invention, the mass ratio of the citrus peel extract to the Leontopodium alpinum extract is 1:1.

[0017] As an embodiment of the present invention, the Scutellaria baicalensis root extract, Leontopodium alpinum extract and citrus peel extract are prepared by fermenting Scutellaria baicalensis root, Leontopodium alpinum and citrus peel with Flos Lonicerae Japonicae Thunb.

[0018] As an embodiment of the present invention, the strains used for fermentation further include Bacillus or / and yeast for symbiotic fermentation.

[0019] As an embodiment of the present invention, the Bacillus is Bacillus subtilis.

[0020] As an embodiment of the present invention, the yeast is Debaryomyces hansenii.

[0021] As an embodiment of the present invention, the soothing and repairing composition is applied to the daily chemical field.

[0022] Adopting the above technical solutions, the present invention has the following beneficial effects:

[0023] 1. Using the solid-state aerobic fermentation method, study from the different plant properties of the substrates.

[0024] 2. Screen and combine from the strain library, select the most suitable combination (Flos Lonicerae Japonicae Thunb. complex or Flos Lonicerae Japonicae Thunb. + other strains), and directionally improve the active substance index.

[0025] 3. Based on the multiple enzymatic hydrolysis and generation of secondary metabolites of the composite strain, enrich the active ingredients and obtain more small molecule polypeptides. Brief Description of the Drawings

[0026] In order to more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following will briefly introduce the drawings required for the description of the specific embodiments or the prior art. Obviously, the drawings in the following description are some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can be obtained based on these drawings.

[0027] Figure 1 This is a picture when performing repair tests on the product provided in Example 1 of the present invention. Specific embodiments

[0028] The following will clearly and completely describe the technical solutions of the present invention. Obviously, the described embodiments are some embodiments of the present invention, rather than all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present invention.

[0029] Preferably, the present invention provides a soothing and repairing composition, which comprises: Scutellaria baicalensis root extract, Leontopodium alpinum extract, citrus peel extract; and the mass ratio of the preparation raw materials is 5:4:4.

[0030] In the present invention, the preparation method of the soothing and repairing composition is as follows:

[0031] Step 1: Strain activation

[0032] Inoculate Eurotium cristatum on a PDA slant and culture at 28°C for 5 - 7 days.

[0033] Step 2: Pretreat citrus peel:

[0034] After drying and crushing the peel at a low temperature of 30°C, put the peel into an extraction tank, control the temperature at 35°C and the pressure at 15 MPa to make CO 2 reach the supercritical state; extract for 60 min, and the CO 2 flow rate is 0.5 L / min, reduce the pressure and temperature, and separate the essential oil and CO 2 ; obtain citrus vegetable oil and citrus pomace;

[0035] Step 3: Pretreat Scutellaria baicalensis root and Leontopodium alpinum

[0036] Preparation of Scutellaria baicalensis powder: Slice the Scutellaria baicalensis root and Leontopodium alpinum, dry (<50°C), and crush to fine powder;

[0037] Matrix preparation: Mix the Scutellaria baicalensis root powder, Leontopodium alpinum powder, and citrus pomace to obtain a mixture. After mixing the mixture and distilled water according to a mass ratio of 1:1, then add 1% ammonium nitrate and 0.5% yeast extract.

[0038] Step 3: Liquid seed culture

[0039] Prepare a liquid medium (water, 5% maltose, 0.3% yeast powder, 0.2% peptone, pH 6.5); scrape the activated Eurotium cristatum on the slant with a sterilized inoculation loop and inoculate it into the liquid medium, and culture it in a shaker at 28°C and 120 rpm for 48 - 72 hours until the bacterial liquid concentration is stable, to obtain an Eurotium cristatum suspension, a Bacillus suspension, and a yeast suspension respectively.

[0040] Step 4: Solid fermentation

[0041] After uniformly spraying the prepared citrus vegetable oil on the substrate; mix the three liquid seed bacterial suspensions according to a mass ratio of 4:0.8:0.2, and then uniformly spray them onto the sterilized and cooled substrate (inoculation amount: 10% of the total amount of the solid substrate); ensure that the substrate is loose and evenly distributed.

[0042] At a temperature of 28 - 30°C; maintain a relative humidity of 70% - 80%; for a time of 5 - 7 days; turn the substrate three times in the morning, afternoon, and evening every day to ensure oxygen supply and uniform fermentation.

[0043] Step 5: Fermentation termination

[0044] After the fermentation is completed, prepare the fermented liquid and solid by high-speed centrifugation; add 50% ethanol to the solid according to a mass ratio of 1:10, and perform ultrasonic extraction for 30 minutes; centrifuge or filter to obtain the supernatant, and mix it with the fermented liquid to obtain the soothing and repairing composition.

[0045] Among them, the Eurotium cristatum is Eurotium cristatum 2099, China Center for Industrial Culture Collection.

[0046] The Bacillus is Bacillus subtilis 10732, China Center for Industrial Culture Collection.

[0047] The yeast is Debaryomyces hansenii, 1266, China Center for Industrial Culture Collection.

[0048] The present invention also provides a sensitive skin repair cream, and according to parts by weight, its preparation raw materials include:

[0049] Component A:

[0050] 1.5 parts of cetearyl olivate

[0051] 1 part of sorbitan olivate

[0052] 0.5 parts of PEG-20 methyl glucoside sesquistearate

[0053] 0.5 parts of tocopherol (vitamin E)

[0054] 2.2 parts of cetearyl alcohol

[0055] 0.3 parts of PEG-100 stearate

[0056] 0.7 parts of glyceryl stearate

[0057] 3 parts of ethylhexyl palmitate

[0058] 5 parts of triethylhexanoin

[0059] 2 parts of squalane

[0060] 1 part of polydimethylsiloxane

[0061] 1 part of tocopheryl acetate

[0062] 0.5 parts of jojoba (SIMMONDSIA CHINENSIS) seed oil

[0063] 0.5 parts of Limnanthes alba seed oil

[0064] Component B:

[0065] 59.08 parts of water

[0066] 0.05 parts of disodium EDTA

[0067] 0.2 parts of allantoin

[0068] 1 part of betaine

[0069] 1 part of xylitol

[0070] 5 parts of glycerol

[0071] 0.1 part of carbomer

[0072] 0.5 parts of acryloyldimethyltaurate / VP copolymer

[0073] 0.05 parts of sodium hyaluronate

[0074] Component C

[0075] 3 parts of water

[0076] 0.3 parts of arginine

[0077] Component D1

[0078] 5 parts of butanediol

[0079] 0.5 parts of p-hydroxyacetophenone

[0080] Component D2

[0081] 0.5 parts of 1,2 - hexanediol

[0082] 1 part of panthenol

[0083] 3 parts of soothing and repairing composition

[0084] 0.02 part of Pelargonium graveolens oil

[0085] The preparation method is as follows:

[0086] 1. Ensure that the machines, containers, equipment used are cleaned and disinfected thoroughly; reserve some water to rinse the oil pan; phase C is pre - dissolved completely; phase D1 is pre - heated slightly (about 75 °C) and dissolved completely, then cooled for standby;

[0087] 2. Add phase A to the oil pan, heat to 82 °C and dissolve completely, keep the temperature constant for 15 minutes;

[0088] 3. Add phase B to the emulsifying pan, heat to 82 °C and dissolve completely, homogenize for 3 - 5 minutes until dissolved, keep the temperature constant for 15 minutes;

[0089] 4. Filter and pump phase A into the emulsifying pan, rinse the oil pan with the reserved water and add it to the emulsifying pan together; homogenize for 10 - 15 minutes, keep stirring at a constant temperature for 20 minutes and then cool down while stirring;

[0090] 5. Cool down to about 70 °C, add the pre - dissolved phase C, stir and neutralize completely; continue to stir and cool down;

[0091] 6. Cool down to about 45 °C, add the pre - dissolved phase D1 and each ingredient of phase D2, stir and mix evenly;

[0092] 7. Cool down to about 38 °C, stop stirring, take samples for inspection, and filter and discharge through a 200 - mesh filter cloth after passing the inspection.

[0093] When studying the properties of plants and the mechanism of skin allergy, the present invention discovers that plant extracts after being fermented by strains are more friendly to allergic skin. The reason is that the metabolites produced after fermentation will undergo some new changes in properties, have a smaller molecular weight, and can also achieve better improvement in the ability of rapid repair. The inventor first applies Eurotium cristatum in the field of skin care products for sensitive skin and discovers that Eurotium cristatum secretes various enzymes during the fermentation process. These enzymes promote the conversion of nutrients by decomposing macromolecular substances. For example, cellulose is decomposed into soluble oligosaccharides or glucose to increase the content of soluble sugars; starch is hydrolyzed into maltose and glucose to provide an energy source for microorganisms; proteins are decomposed into small peptides and amino acids to release nitrogen sources and improve the flavor of food; fats are decomposed to generate free fatty acids, giving food a unique aroma. Eurotium cristatum synthesizes various bioactive substances in the secondary metabolism stage.

[0094] The inventor discovers that when using Eurotium cristatum to ferment and extract the root of Scutellaria baicalensis Georgi and Leontopodium alpinum Cass., the water solubility of the obtained extract decreases and more oil-soluble substances appear. Secondly, it is found that when the composition obtained by fermenting solely with Eurotium cristatum is applied to skin care products, it has a strong immediate repair effect, but the long-term repair effect is weak. The possible reason is that the polysaccharides that produce more repair effects are consumed during fermentation. Therefore, the inventor uses multiple strains for fermentation, which can effectively improve the above phenomenon.

[0095] The following further explains and illustrates the present invention in combination with specific embodiments.

[0096] Example 1

[0097] This example provides a soothing and repairing composition, which comprises: the extract of the root of Scutellaria baicalensis Georgi, the extract of Leontopodium alpinum Cass., and the extract of citrus peel; and the mass ratio of the preparation raw materials is 5:4:4.

[0098] In this example, the preparation method of the soothing and repairing composition is as follows:

[0099] Step 1: Activation of the strain

[0100] Inoculate Eurotium cristatum on a PDA slant and culture at 28 °C for 5 days.

[0101] Step 2: Pretreatment of citrus peel:

[0102] After drying and crushing the peel at a low temperature of 30 °C, put the peel into an extraction tank, control the temperature at 35 °C and the pressure at 15 MPa to make CO 2 reach the supercritical state; extract for 60 min, and the flow rate of CO 2 is 0.5 L / min. Reduce the pressure and temperature, and separate the essential oil and CO 2 ; obtain citrus vegetable oil and citrus pomace;

[0103] Step 3: Pretreatment of Scutellaria baicalensis Georgi roots and Leontopodium alpinum

[0104] Preparation of Scutellaria baicalensis Georgi powder: Slice the Scutellaria baicalensis Georgi roots and Leontopodium alpinum, dry them (<50°C), and crush them into fine powder;

[0105] Matrix preparation: Mix the Scutellaria baicalensis Georgi root powder, Leontopodium alpinum powder, and citrus fruit pomace to obtain a mixture. After mixing the mixture with distilled water at a mass ratio of 1:1, add 1 wt% ammonium nitrate and 0.5 wt% yeast extract.

[0106] Step 3: Liquid seed culture

[0107] Prepare a liquid medium (water, 5% maltose, 0.3% yeast powder, 0.2% peptone, pH 6.5); Use a sterilized inoculation loop to scrape the activated Eurotium cristatum on the slant and inoculate it into the liquid medium. Incubate it in a shaker at 28°C and 120 rpm for 60 hours until the bacterial liquid concentration is stable to obtain an Eurotium cristatum suspension, a Bacillus suspension, and a yeast suspension respectively.

[0108] Step 4: Solid fermentation

[0109] After uniformly spraying the prepared citrus vegetable oil on the matrix; Mix the three liquid seed bacterial suspensions according to a mass ratio of 4:0.8:0.2, and then uniformly spray them onto the sterilized and cooled matrix (inoculation amount: 10% of the total amount of the solid matrix); Ensure that the matrix is loose and evenly distributed.

[0110] At a temperature of 28°C; Maintain a relative humidity of 70%; Time: 7 days; Turn the matrix three times in the morning, afternoon, and evening every day to ensure oxygen supply and uniform fermentation.

[0111] Step 5: Fermentation termination

[0112] After the fermentation is completed, high-speed centrifugation is used to obtain the fermented liquid and solid; Add 50% ethanol to the solid at a mass ratio of 1:10, and perform ultrasonic extraction for 30 minutes; Centrifuge or filter to obtain the supernatant, and mix it with the fermented liquid to obtain the soothing and repairing composition.

[0113] Among them, the Eurotium cristatum is Eurotium cristatum 2099, China Center for Industrial Culture Collection. 2099, China Center for Industrial Culture Collection.

[0114] The Bacillus genus is Bacillus subtilis 10732, China Center for Industrial Culture Collection. 10732, China Center for Industrial Culture Collection.

[0115] The yeast is Debaryomyces hansenii, 1266, China National Center for Industrial Culture Collection

[0116] This embodiment also provides a sensitive repair cream, and its preparation raw materials include, by weight:

[0117] Component A:

[0118] Cetearyl Olivate 1.5 parts

[0119] Sorbitan Olivate 1 part

[0120] PEG-20 Methyl Glucose Sesquistearate 0.5 part

[0121] Tocopherol (Vitamin E) 0.5 part

[0122] Cetearyl Alcohol 2.2 parts

[0123] PEG-100 Stearate 0.3 part

[0124] Glyceryl Stearate 0.7 part

[0125] Ethylhexyl Palmitate 3 parts

[0126] Tris(ethylhexanoate) Glyceride 5 parts

[0127] Squalane 2 parts

[0128] Polydimethylsiloxane 1 part

[0129] Tocopheryl Acetate 1 part

[0130] Jojoba (SIMMONDSIA CHINENSIS) Seed Oil 0.5 part Limnanthes Alba Seed Oil 0.5 part Component B:

[0131] Water 59.08 parts

[0132] Disodium EDTA 0.05 part

[0133] Allantoin 0.2 part

[0134] Betaine 1 part

[0135] Xylitol 1 part

[0136] Glycerol 5 parts

[0137] Carbomer 0.1 part

[0138] Ammonium Acryloyldimethyltaurate / VP Copolymer 0.5 part Sodium Hyaluronate 0.05 part

[0139] Component C

[0140] Water 3 parts

[0141] 0.3 parts of arginine

[0142] Component D1

[0143] 5 parts of butanediol

[0144] 0.5 part of p - hydroxyacetophenone

[0145] Component D2

[0146] 0.5 part of 1,2 - hexanediol

[0147] 1 part of panthenol

[0148] 3 parts of the soothing and repairing composition provided by this example

[0149] 0.02 part of Pelargonium graveolens oil.

[0150] 1. Ensure that the machines, containers, equipment used are cleaned and disinfected thoroughly; reserve some water to rinse the oil pan; phase C is pre - dissolved completely; phase D1 is pre - heated slightly (about 75 degrees) and dissolved completely, then cooled for standby;

[0151] 2. Add phase A to the oil pan, heat to 82 degrees and dissolve completely, keep the temperature constant for 15 minutes;

[0152] 3. Add phase B to the emulsifying pan, heat to 82 degrees and dissolve completely, homogenize for 3 - 5 minutes to dissolve, keep the temperature constant for 15 minutes;

[0153] 4. Filter and pump phase A into the emulsifying pan, rinse the oil pan with the reserved water and add it to the emulsifying pan together; homogenize for 10 - 15 minutes, keep stirring at a constant temperature for 20 minutes, then stir to lower the temperature;

[0154] 5. Lower the temperature to about 70 degrees, add the pre - dissolved phase C, stir and neutralize completely; continue to stir and lower the temperature;

[0155] 6. Lower the temperature to about 45 degrees, add the pre - dissolved components of phase D1 and phase D2 respectively, stir and mix evenly;

[0156] 7. Lower the temperature to about 38 degrees, stop stirring, take samples for inspection, and filter through a 200 - mesh filter cloth for discharging after passing the inspection.

[0157] Example 2

[0158] This example provides a soothing and repairing composition, and the composition contains: Scutellaria baicalensis root extract, Leontopodium alpinum extract, citrus peel extract; and the mass ratio of the preparation raw materials is 5:4:4.

[0159] In this example, the preparation method of the soothing and repairing composition is as follows:

[0160] Step 1: Activation of strains

[0161] Inoculate Eurotium cristatum on the PDA slant and culture at 28°C for 5 days.

[0162] Step 2: Pretreatment of citrus peel

[0163] After drying and crushing the peel at a low temperature of 30°C, put the peel into the extraction tank, control the temperature at 35°C and the pressure at 15 MPa to make CO 2 reach the supercritical state; extract for 60 min, and the CO 2 flow rate is 0.5 L / min, reduce the pressure and temperature, and separate the essential oil and CO 2 ; obtain citrus vegetable oil and citrus pomace;

[0164] Step 3: Pretreatment of Scutellaria baicalensis root and Leontopodium alpinum

[0165] Preparation of Scutellaria baicalensis powder: Slice the Scutellaria baicalensis root and Leontopodium alpinum, dry (<50°C), and crush to fine powder;

[0166] Matrix preparation: Mix the Scutellaria baicalensis root powder, Leontopodium alpinum powder, and citrus pomace to obtain a mixture. After mixing the mixture and distilled water according to a mass ratio of 1:1, then add 1 wt% ammonium nitrate and 0.5 wt% yeast extract.

[0167] Step 3: Liquid seed culture

[0168] Prepare a liquid medium (water, 5% maltose, 0.3% yeast powder, 0.2% peptone, pH 6.5); use a sterilized inoculation loop to scrape the activated Eurotium cristatum on the slant and inoculate it into the liquid medium. Culture in a shaker at 28°C and 120 rpm for 60 hours until the bacterial liquid concentration is stable to obtain an Eurotium cristatum suspension and a Bacillus suspension respectively.

[0169] Step 4: Solid fermentation

[0170] After uniformly spraying the prepared citrus vegetable oil on the matrix; mix the three liquid seed suspensions according to a mass ratio of 4:0.8, and then uniformly spray them onto the sterilized and cooled matrix (inoculation amount: 10% of the total amount of the solid matrix); ensure that the matrix is loose and evenly distributed.

[0171] At a temperature of 28°C; maintain a relative humidity of 70%; time: 7 days; turn the matrix three times in the morning, noon, and evening every day to ensure oxygen supply and uniform fermentation.

[0172] Step 5: Termination of fermentation

[0173] After fermentation, high-speed centrifugation is used to obtain the fermented liquid and solid; the solid is added to 50% ethanol at a mass ratio of 1:10, and ultrasonic extraction is carried out for 30 minutes; the supernatant is taken by centrifugation or filtration and mixed with the fermented liquid to obtain the soothing and repairing composition.

[0174] Among them, the Eurotium cristatum is Eurotium cristatum 2099, China Center for Industrial Culture Collection of Microorganisms.

[0175] The Bacillus genus is Bacillus subtilis 10732, China Center for Industrial Culture Collection of Microorganisms.

[0176] This example also provides a sensitive skin repair cream, and according to weight parts, its preparation raw materials include:

[0177] Component A:

[0178] Cetearyl Olivate 1.5 parts

[0179] Sorbitan Olivate 1 part

[0180] PEG-20 Methyl Glucose Sesquistearate 0.5 part

[0181] Tocopherol (Vitamin E) 0.5 part

[0182] Cetearyl Alcohol 2.2 parts

[0183] PEG-100 Stearate 0.3 part

[0184] Glyceryl Stearate 0.7 part

[0185] Ethylhexyl Palmitate 3 parts

[0186] Triethylhexanoin 5 parts

[0187] Squalane 2 parts

[0188] Polydimethylsiloxane 1 part

[0189] Tocopheryl Acetate 1 part

[0190] Jojoba (SIMMONDSIA CHINENSIS) Seed Oil 0.5 part

[0191] Limnanthes Alba Seed Oil 0.5 part

[0192] Component B:

[0193] Water 59.08 parts

[0194] 0.05 parts of disodium EDTA

[0195] 0.2 parts of allantoin

[0196] 1 part of betaine

[0197] 1 part of xylitol

[0198] 5 parts of glycerol

[0199] 0.1 part of carbomer

[0200] 0.5 part of acryloyldimethyltaurate / VP copolymer

[0201] 0.05 part of sodium hyaluronate

[0202] Component C

[0203] 3 parts of water

[0204] 0.3 part of arginine

[0205] Component D1

[0206] 5 parts of butanediol

[0207] 0.5 part of p-hydroxyacetophenone

[0208] Component D2

[0209] 0.5 part of 1,2-hexanediol

[0210] 1 part of panthenol

[0211] 3 parts of the soothing and repairing composition provided by this example

[0212] 0.02 part of Pelargonium graveolens oil.

[0213] 1. Ensure that the machines, containers, equipment used are cleaned and disinfected; reserve some water to rinse the oil pan; phase C is pre-dissolved completely; phase D1 is pre-heated slightly (about 75 degrees) and dissolved completely, then cooled for standby;

[0214] 2. Add phase A to the oil pan, heat to 82 degrees and dissolve completely, keep the temperature constant for 15 minutes;

[0215] 3. Add phase B to the emulsifying pan, heat to 82 degrees and dissolve completely, homogenize for 3 - 5 minutes to dissolve, keep the temperature constant for 15 minutes;

[0216] 4. Filter and pump phase A into the emulsifying pan, rinse the oil pan with the reserved water and add it to the emulsifying pan together; homogenize for 10 - 15 minutes, keep stirring at a constant temperature for 20 minutes and then cool down while stirring;

[0217] 5. Cool down to about 70 degrees, add the pre-dissolved phase C, stir and neutralize completely; continue to stir and cool down;

[0218] 6. Cool down to about 45 °C, add the pre-dissolved D1 phase and D2 phase materials respectively, and stir and mix evenly.

[0219] 7. Cool down to about 38 °C, stop stirring, take samples for inspection, and filter through a 200-mesh filter cloth after passing the inspection.

[0220] Example 3

[0221] This example provides a soothing and repairing composition, which comprises: Scutellaria baicalensis root extract, Leontopodium alpinum extract, citrus peel extract; and the mass ratio of the preparation raw materials is 5:4:4.

[0222] In this example, the preparation method of the soothing and repairing composition is as follows:

[0223] Step 1: Strain activation

[0224] Inoculate Eurotium cristatum on a PDA slant and culture at 28 °C for 5 days.

[0225] Step 2: Pretreatment of citrus peel:

[0226] After drying and crushing the peel at a low temperature of 30 °C, put the peel into an extraction tank, control the temperature at 35 °C and the pressure at 15 MPa to make CO 2 reach the supercritical state; extract for 60 min, and the CO 2 flow rate is 0.5 L / min, reduce the pressure and temperature, and separate the essential oil and CO 2 ; obtain citrus vegetable oil and citrus pomace;

[0227] Step 3: Pretreatment of Scutellaria baicalensis root and Leontopodium alpinum

[0228] Preparation of Scutellaria baicalensis powder: Cut the Scutellaria baicalensis root and Leontopodium alpinum into slices, dry (<50 °C), and crush them into fine powder;

[0229] Matrix preparation: Mix the Scutellaria baicalensis root powder, Leontopodium alpinum powder, and citrus pomace to obtain a mixture. After mixing the mixture and distilled water according to a mass ratio of 1:1, then add 1 wt% ammonium nitrate and 0.5 wt% yeast extract.

[0230] Step 3: Liquid seed culture

[0231] Prepare a liquid medium (water, 5% maltose, 0.3% yeast powder, 0.2% peptone, pH 6.5); scrape the slant-activated Eurotium cristatum with a sterilized inoculation loop and inoculate it into the liquid medium. Culture in a shaker at 28 °C and 120 rpm for 60 hours until the bacterial liquid concentration is stable to obtain an Eurotium cristatum suspension respectively.

[0232] Step 4: Solid fermentation

[0233] After uniformly spraying the prepared citrus vegetable oil on the substrate, uniformly spray the liquid seed bacteria suspension onto the sterilized and cooled substrate (inoculation amount: 10% of the total amount of the solid substrate); ensure that the substrate is loose and evenly distributed.

[0234] At a temperature of 28 °C, maintain a relative humidity of 70%, for a time of 7 days, and turn the substrate three times in the morning, middle, and evening every day to ensure oxygen supply and uniform fermentation.

[0235] Step 5: Fermentation termination

[0236] After the fermentation is completed, prepare the fermented liquid and solid by high-speed centrifugation; add the solid to 50% ethanol at a mass ratio of 1:10, and ultrasonically extract for 30 minutes; centrifuge or filter to obtain the supernatant, and mix it with the fermented liquid to obtain the soothing and repairing composition.

[0237] Among them, the Eurotium cristatum is Eurotium cristatum 2099, China Center for Industrial Culture Collection.

[0238] This example also provides a sensitive skin repair cream, and according to the weight parts, its preparation raw materials include:

[0239] Component A:

[0240] Cetearyl Olivate 1.5 parts

[0241] Sorbitan Olivate 1 part

[0242] PEG-20 Methyl Glucose Sesquistearate 0.5 part

[0243] Tocopherol (vitamin E) 0.5 part

[0244] Cetearyl Alcohol 2.2 parts

[0245] PEG-100 Stearate 0.3 part

[0246] Glyceryl Stearate 0.7 part

[0247] Ethylhexyl Palmitate 3 parts

[0248] Triethylhexanoin 5 parts

[0249] Squalane 2 parts

[0250] Polydimethylsiloxane 1 part

[0251] Tocopheryl Acetate 1 part

[0252] Jojoba (SIMMONDSIA CHINENSIS) Seed Oil 0.5 part

[0253] 0.5 parts of Limnanthes Alba seed oil

[0254] Component B:

[0255] 59.08 parts of water

[0256] 0.05 part of disodium EDTA

[0257] 0.2 part of allantoin

[0258] 1 part of betaine

[0259] 1 part of xylitol

[0260] 5 parts of glycerol

[0261] 0.1 part of carbomer

[0262] 0.5 part of acryloyldimethyltaurate / VP copolymer

[0263] 0.05 part of sodium hyaluronate

[0264] Component C

[0265] 3 parts of water

[0266] 0.3 part of arginine

[0267] Component D1

[0268] 5 parts of butanediol

[0269] 0.5 part of p-hydroxyacetophenone

[0270] Component D2

[0271] 0.5 part of 1,2-hexanediol

[0272] 1 part of panthenol

[0273] 3 parts of the soothing and repairing composition provided in this example

[0274] 0.02 part of Pelargonium graveolens oil

[0275] 1. Ensure that the machines, containers, and equipment used are cleaned and disinfected; reserve some water to rinse the oil pan; phase C is pre-dissolved completely; phase D1 is pre-heated (about 75 degrees) and dissolved completely, then cooled for standby;

[0276] 2. Add phase A to the oil pan, heat to 82 degrees and dissolve completely, then keep the temperature constant for 15 minutes;

[0277] 3. Add phase B to the emulsifying pan, heat to 82 degrees and dissolve completely, homogenize for 3 - 5 minutes to dissolve, then keep the temperature constant for 15 minutes;

[0278] 4. Filter and pump the A phase into the emulsifying pan, rinse the oil pan with the reserved part of water and add it to the emulsifying pan together; Homogenize for 10 - 15 minutes, stir at a constant temperature for 20 minutes and then cool down while stirring.

[0279] 5. Cool down to about 70 °C, add the pre-dissolved C phase and stir until neutralized completely; Continue to stir and cool down.

[0280] 6. Cool down to about 45 °C, add each material of the pre-dissolved D1 phase and D2 phase and stir to mix evenly.

[0281] 7. Cool down to about 38 °C, stop stirring, take a sample for inspection, and filter and discharge through a 200-mesh filter cloth after passing the inspection.

[0282] Example 4

[0283] This example provides a soothing and repairing composition, and the composition contains: Scutellaria baicalensis root extract, Leontopodium alpinum extract, citrus peel extract; And the mass ratio of the preparation raw materials is 5:4:4.

[0284] In this example, the preparation method of the soothing and repairing composition is as follows:

[0285] Step 1: Strain activation

[0286] Inoculate Eurotium cristatum on the PDA slant and culture at 28 °C for 5 days.

[0287] Step 2: Pretreat the citrus peel:

[0288] After drying and crushing the peel at a low temperature of 30 °C, put the peel into the extraction tank, control the temperature at 35 °C and the pressure at 15 MPa to make CO 2 reach the supercritical state; Extract for 60 min, and the CO 2 flow rate is 0.5 L / min, reduce the pressure and cool down, and separate the essential oil and CO 2 ; Obtain citrus vegetable oil and citrus pomace.

[0289] Step 3: Pretreat Scutellaria baicalensis root and Leontopodium alpinum

[0290] Preparation of Scutellaria baicalensis powder: Slice the Scutellaria baicalensis root and Leontopodium alpinum, dry (<50 °C), and crush to fine powder.

[0291] Matrix preparation: Mix the Scutellaria baicalensis root powder, Leontopodium alpinum powder and citrus pomace to obtain a mixture, mix the mixture and distilled water according to the mass ratio of 1:1, and then add 1 wt% ammonium nitrate and 0.5 wt% yeast extract.

[0292] Step 3: Liquid seed culture

[0293] Prepare a liquid medium (water, 5% maltose, 0.3% yeast powder, 0.2% peptone, pH 6.5); use a sterilized inoculation loop to scrape the slant-activated Eurotium cristatum and inoculate it into the liquid medium. Incubate in a shaker at 28°C and 120 rpm for 60 hours until the bacterial liquid concentration is stable to obtain an Eurotium cristatum suspension, a Bacillus suspension, and a yeast suspension respectively.

[0294] Step 4: Solid fermentation

[0295] After uniformly spraying the prepared citrus vegetable oil on the substrate; mix the three liquid seed bacterial suspensions according to a mass ratio of 3:2:2 and then uniformly spray them onto the sterilized and cooled substrate (inoculation amount: 10% of the total amount of the solid substrate); ensure that the substrate is loose and evenly distributed.

[0296] At a temperature of 28°C; maintain a relative humidity of 70%; time: 7 days; turn the substrate three times every morning, noon, and evening to ensure oxygen supply and uniform fermentation.

[0297] Step 5: Fermentation termination

[0298] After the fermentation is completed, prepare the fermented liquid and solid by high-speed centrifugation; add 50% ethanol to the solid according to a mass ratio of 1:10 and ultrasonically extract for 30 minutes; centrifuge or filter to obtain the supernatant and mix it with the fermented liquid to obtain the soothing and repairing composition.

[0299] Among them, the Eurotium cristatum is Eurotium cristatum 2099, China Center for Industrial Culture Collection.

[0300] The Bacillus is Bacillus subtilis 10732, China Center for Industrial Culture Collection.

[0301] The yeast is Debaryomyces hansenii, 1266, China Center for Industrial Culture Collection.

[0302] This example also provides a sensitive skin repair cream, and according to the weight parts, its preparation raw materials include:

[0303] Component A:

[0304] 1.5 parts of cetearyl olivate

[0305] 1 part of sorbitan olivate

[0306] 0.5 part of PEG-20 methyl glucose sesquistearate

[0307] Tocopherol (Vitamin E) 0.5 parts

[0308] Cetearyl alcohol 2.2 parts

[0309] PEG - 100 stearate 0.3 parts

[0310] Glyceryl stearate 0.7 parts

[0311] Ethylhexyl palmitate 3 parts

[0312] Tris(ethylhexanoic acid) glyceride 5 parts

[0313] Squalane 2 parts

[0314] Polydimethylsiloxane 1 part

[0315] Tocopheryl acetate 1 part

[0316] Jojoba (SIMMONDSIA CHINENSIS) seed oil 0.5 parts

[0317] Limnanthes alba seed oil 0.5 parts

[0318] Component B:

[0319] Water 59.08 parts

[0320] Disodium EDTA 0.05 parts

[0321] Allantoin 0.2 parts

[0322] Betaine 1 part

[0323] Xylitol 1 part

[0324] Glycerol 5 parts

[0325] Carbomer 0.1 part

[0326] Acryloyldimethyltaurate / VP copolymer 0.5 parts

[0327] Sodium hyaluronate 0.05 parts

[0328] Component C

[0329] Water 3 parts

[0330] Arginine 0.3 parts

[0331] Component D1

[0332] Butylene glycol 5 parts

[0333] p - Hydroxyacetophenone 0.5 parts

[0334] Component D2

[0335] 1,2 - Hexanediol 0.5 parts

[0336] 1 part of panthenol

[0337] 3 parts of the soothing and repairing composition provided by this embodiment

[0338] 0.02 part of Pelargonium graveolens oil

[0339] 1. Ensure that the machines, containers, and equipment used are cleaned and disinfected; reserve some water to rinse the oil pan; phase C is pre-dissolved completely; phase D1 is pre-heated (about 75 °C) and heated until completely dissolved, then cooled for standby;

[0340] 2. Add phase A to the oil pan, heat to 82 °C until completely dissolved, and keep the temperature constant for 15 minutes;

[0341] 3. Add phase B to the emulsifying pan, heat to 82 °C until completely dissolved, homogenize for 3 - 5 minutes until dissolved, and keep the temperature constant for 15 minutes;

[0342] 4. Filter and pump phase A into the emulsifying pan, rinse the oil pan with the reserved water and add it to the emulsifying pan together; homogenize for 10 - 15 minutes, stir at a constant temperature for 20 minutes, and then cool while stirring;

[0343] 5. Cool to about 70 °C, add the pre-dissolved phase C, and stir until completely neutralized; continue to stir and cool;

[0344] 6. Cool to about 45 °C, add the pre-dissolved phase D1 and each ingredient of phase D2, and stir to mix evenly;

[0345] 7. Cool to about 38 °C, stop stirring, take a sample for inspection, and filter and discharge through a 200-mesh filter cloth after passing the inspection.

[0346] Example 5

[0347] This example provides a soothing and repairing composition, and the composition contains: Scutellaria baicalensis root extract, Leontopodium alpinum extract, citrus peel extract; and the mass ratio of the preparation raw materials is 5:4:4.

[0348] In this example, the preparation method of the soothing and repairing composition is as follows:

[0349] Step 1: Strain activation

[0350] Inoculate Eurotium cristatum on the PDA slant and culture at 28 °C for 5 days.

[0351] Step 2: Pretreatment of Scutellaria baicalensis root and Leontopodium alpinum

[0352] Preparation of Scutellaria baicalensis powder: Slice the Scutellaria baicalensis root, citrus peel, and Leontopodium alpinum, dry (<50 °C), and crush to fine powder;

[0353] Matrix preparation: Mix the Scutellaria baicalensis root powder, Leontopodium alpinum Cass. powder, and citrus peel to obtain a mixture. After mixing the mixture and distilled water at a mass ratio of 1:1, add 1 wt% ammonium nitrate and 0.5 wt% yeast extract.

[0354] Step 3: Liquid seed culture

[0355] Prepare a liquid medium (water, 5% maltose, 0.3% yeast powder, 0.2% peptone, pH 6.5); use a sterilized inoculation loop to scrape the activated Eurotium cristatum on the slant and inoculate it into the liquid medium. Incubate in a shaker at 28°C and 120 rpm for 60 hours until the bacterial liquid concentration is stable to obtain an Eurotium cristatum suspension, a Bacillus suspension, and a yeast suspension respectively.

[0356] Step 4: Solid fermentation

[0357] Mix the three liquid seed bacterial suspensions at a mass ratio of 4:0.8:0.2, and evenly spray them onto the sterilized and cooled matrix (inoculation amount: 10% of the total amount of the solid matrix); ensure that the matrix is loose and evenly distributed.

[0358] At a temperature of 28°C; maintain a relative humidity of 70%; time: 7 days; turn the matrix three times in the morning, afternoon, and evening every day to ensure oxygen supply and uniform fermentation.

[0359] Step 5: Fermentation termination

[0360] After the fermentation is completed, prepare the fermented liquid and solid by high-speed centrifugation; add 50% ethanol to the solid at a mass ratio of 1:10, and perform ultrasonic extraction for 30 minutes; centrifuge or filter to obtain the supernatant, and mix it with the fermented liquid to obtain the soothing and repairing composition.

[0361] Among them, the Eurotium cristatum is Eurotium cristatum 2099, China Center for Industrial Culture Collection.

[0362] The Bacillus genus is Bacillus subtilis 10732, China Center for Industrial Culture Collection.

[0363] The yeast is Debaryomyces hansenii, 1266, China Center for Industrial Culture Collection.

[0364] This example also provides a sensitive skin repair cream, and according to weight parts, its preparation raw materials include:

[0365] Component A:

[0366] 1.5 parts of cetearyl olivate

[0367] 1 part of sorbitan olivate

[0368] 0.5 part of PEG - 20 methylglucose sesquistearate

[0369] 0.5 part of tocopherol (vitamin E)

[0370] 2.2 parts of cetearyl alcohol

[0371] 0.3 part of PEG - 100 stearate

[0372] 0.7 part of glyceryl stearate

[0373] 3 parts of ethylhexyl palmitate

[0374] 5 parts of triethylhexanoin

[0375] 2 parts of squalane

[0376] 1 part of polydimethylsiloxane

[0377] 1 part of tocopheryl acetate

[0378] 0.5 part of jojoba (SIMMONDSIA CHINENSIS) seed oil, 0.5 part of limnanthes alba (meadowfoam) seed oil, Component B:

[0379] 59.08 parts of water

[0380] 0.05 part of disodium EDTA

[0381] 0.2 part of allantoin

[0382] 1 part of betaine

[0383] 1 part of xylitol

[0384] 5 parts of glycerol

[0385] 0.1 part of carbomer

[0386] 0.5 part of acryloyldimethyltaurate / VP copolymer, 0.05 part of sodium hyaluronate

[0387] Component C

[0388] 3 parts of water

[0389] 0.3 part of arginine

[0390] Component D1

[0391] 5 parts of butanediol

[0392] 0.5 part of p - hydroxyacetophenone

[0393] Component D2

[0394] 0.5 part of 1,2 - hexanediol

[0395] 1 part of panthenol

[0396] 3 parts of the soothing and repairing composition provided by this example

[0397] 0.02 part of Pelargonium graveolens oil

[0398] 1. Ensure that the machines, containers, equipment used are cleaned and disinfected thoroughly; reserve some water to rinse the oil pan; phase C is pre - dissolved completely; phase D1 is pre - heated slightly (about 75 °C) and dissolved completely, then cooled for standby.

[0399] 2. Add phase A to the oil pan, heat to 82 °C and dissolve completely, keep the temperature constant for 15 minutes.

[0400] 3. Add phase B to the emulsifying pan, heat to 82 °C and dissolve completely, homogenize for 3 - 5 minutes to dissolve, keep the temperature constant for 15 minutes.

[0401] 4. Filter and pump phase A into the emulsifying pan, rinse the oil pan with the reserved water and add it to the emulsifying pan together; homogenize for 10 - 15 minutes, keep stirring at a constant temperature for 20 minutes, and then cool down while stirring.

[0402] 5. Cool down to about 70 °C, add the pre - dissolved phase C, and stir until neutralized completely; continue to stir and cool down.

[0403] 6. Cool down to about 45 °C, add the pre - dissolved phase D1 and each ingredient of phase D2, and stir to mix evenly.

[0404] 7. Cool down to about 38 °C, stop stirring, take a sample for inspection, and filter through a 200 - mesh filter cloth for discharging after passing the inspection.

[0405] Example 6

[0406] This example provides a soothing and repairing composition, and the composition contains: Scutellaria baicalensis root extract, Leontopodium alpinum extract, and the mass ratio of the preparation raw materials is 5:4.

[0407] In this example, the preparation method of the soothing and repairing composition is as follows:

[0408] Step 1: Strain activation

[0409] Inoculate Eurotium cristatum on the PDA slant and culture at 28 °C for 5 days.

[0410] Step 2: Pretreatment of Scutellaria baicalensis root and Leontopodium alpinum

[0411] Preparation of Scutellaria baicalensis powder: Cut the roots of Scutellaria baicalensis and Leontopodium alpinum, dry them at a temperature below 50°C, and pulverize them into fine powder.

[0412] Matrix preparation: Mix the powder of Scutellaria baicalensis roots and the powder of Leontopodium alpinum to obtain a mixture. After mixing the mixture and distilled water at a mass ratio of 1:1, add 1 wt% ammonium nitrate and 0.5 wt% yeast extract.

[0413] Step 3: Liquid seed culture

[0414] Prepare a liquid medium (water, 5% maltose, 0.3% yeast powder, 0.2% peptone, pH 6.5); Use a sterilized inoculation loop to scrape the activated Eurotium cristatum on the slant and inoculate it into the liquid medium. Incubate it in a shaker at 28°C and 120 rpm for 60 hours until the concentration of the bacterial liquid is stable, and obtain the suspension of Eurotium cristatum, the suspension of Bacillus, and the suspension of yeast respectively.

[0415] Step 4: Solid fermentation

[0416] After evenly spraying the prepared citrus vegetable oil on the matrix; Mix the three liquid seed bacterial suspensions according to a mass ratio of 4:0.8:0.2, and then evenly spray them onto the sterilized and cooled matrix (inoculation amount: 10% of the total amount of the solid matrix); Ensure that the matrix is loose and evenly distributed.

[0417] At a temperature of 28°C; Maintain a relative humidity of 70%; Time: 7 days; Turn the matrix three times in the morning, middle, and evening every day to ensure oxygen supply and uniform fermentation.

[0418] Step 5: Fermentation termination

[0419] After the fermentation is completed, prepare the fermented liquid and solid by high-speed centrifugation; Add 50% ethanol to the solid at a mass ratio of 1:10, and ultrasonically extract for 30 minutes; Centrifuge or filter to obtain the supernatant, and mix it with the fermented liquid to obtain the soothing and repairing composition.

[0420] Among them, the Eurotium cristatum is Eurotium cristatum 2099, China Center for Industrial Culture Collection. 2099, China Center for Industrial Culture Collection.

[0421] The Bacillus is Bacillus subtilis 10732, China Center for Industrial Culture Collection. 10732, China Center for Industrial Culture Collection.

[0422] The yeast is Debaryomyces hansenii 1266, China Center for Industrial Culture Collection. 1266, China Center for Industrial Culture Collection.

[0423] This embodiment also provides a sensitive skin repair cream. In terms of parts by weight, its preparation raw materials include:

[0424] Component A:

[0425] Cetearyl Olivate 1.5 parts

[0426] Sorbitan Olivate 1 part

[0427] PEG-20 Methyl Glucose Sesquistearate 0.5 part

[0428] Tocopherol (Vitamin E) 0.5 part

[0429] Cetearyl Alcohol 2.2 parts

[0430] PEG-100 Stearate 0.3 part

[0431] Glyceryl Stearate 0.7 part

[0432] Ethylhexyl Palmitate 3 parts

[0433] Triethylhexanoin 5 parts

[0434] Squalane 2 parts

[0435] Polydimethylsiloxane 1 part

[0436] Tocopheryl Acetate 1 part

[0437] Jojoba (SIMMONDSIA CHINENSIS) Seed Oil 0.5 part

[0438] Limnanthes Alba Seed Oil 0.5 part

[0439] Component B:

[0440] Water 59.08 parts

[0441] Disodium EDTA 0.05 part

[0442] Allantoin 0.2 part

[0443] Betaine 1 part

[0444] Xylitol 1 part

[0445] Glycerin 5 parts

[0446] Carbomer 0.1 part

[0447] Ammonium Acryloyldimethyltaurate / VP Copolymer 0.5 part

[0448] Sodium Hyaluronate 0.05 part

[0449] Component C

[0450] 3 parts of water

[0451] 0.3 part of arginine

[0452] Component D1

[0453] 5 parts of butanediol

[0454] 0.5 part of p - hydroxyacetophenone

[0455] Component D2

[0456] 0.5 part of 1,2 - hexanediol

[0457] 1 part of panthenol

[0458] 3 parts of the soothing and repairing composition provided in this example

[0459] 0.02 part of Pelargonium graveolens oil.

[0460] 1. Ensure that the machines, containers, equipment used are cleaned and disinfected thoroughly; reserve a part of the water to rinse the oil pan; phase C is pre - dissolved completely; phase D1 is pre - heated slightly (about 75 °C) and dissolved completely, then cooled for standby;

[0461] 2. Add phase A to the oil pan, heat to 82 °C and dissolve completely, keep the temperature constant for 15 minutes;

[0462] 3. Add phase B to the emulsifying pan, heat to 82 °C and dissolve completely, homogenize for 3 - 5 minutes to dissolve, keep the temperature constant for 15 minutes;

[0463] 4. Filter and pump phase A into the emulsifying pan, rinse the oil pan with the reserved part of the water and add it to the emulsifying pan together; homogenize for 10 - 15 minutes, stir at a constant temperature for 20 minutes, and then cool down while stirring;

[0464] 5. Cool down to about 70 °C, add the pre - dissolved phase C, and stir until neutralized completely; continue to stir and cool down;

[0465] 6. Cool down to about 45 °C, add the pre - dissolved components of phase D1 and phase D2 respectively, and stir to mix evenly;

[0466] 7. Cool down to about 38 °C, stop stirring, take a sample for inspection, and filter and discharge through a 200 - mesh filter cloth after passing the inspection.

[0467] Example 7

[0468] This example provides a soothing and repairing composition, and the composition contains: Scutellaria baicalensis root extract, Leontopodium alpinum extract, citrus peel extract; and the mass ratio of the preparation raw materials is 5:4:4.

[0469] In this example, the preparation method of the soothing and repairing composition is as follows:

[0470] Step 1: Strain activation

[0471] Inoculate Eurotium cristatum on a PDA slant and culture at 28 °C for 5 days.

[0472] Step 2: Pretreatment of citrus peel

[0473] After drying and crushing the peel at a low temperature of 30 °C, put the peel into an extraction tank, control the temperature at 35 °C and the pressure at 15 MPa to make CO 2 reach the supercritical state; extract for 60 min, and the CO 2 flow rate is 0.5 L / min. Reduce the pressure and temperature, and separate the essential oil and CO 2 ; Obtain citrus vegetable oil and citrus pomace;

[0474] Step 3: Pretreatment of Scutellaria baicalensis root and Leontopodium alpinum

[0475] Preparation of Scutellaria baicalensis powder: Slice the Scutellaria baicalensis root and Leontopodium alpinum, dry (<50 °C), and crush to fine powder;

[0476] Matrix preparation: Mix the Scutellaria baicalensis root powder, Leontopodium alpinum powder, and citrus pomace to obtain a mixture. After mixing the mixture and distilled water in a mass ratio of 1:1, then add 1 wt% ammonium nitrate and 0.5 wt% yeast extract.

[0477] Step 3: Liquid seed culture

[0478] Prepare a liquid medium (water, 5% maltose, 0.3% yeast powder, 0.2% peptone, pH 6.5); Use a sterilized inoculation loop to scrape the activated Eurotium cristatum on the slant and inoculate it into the liquid medium. Culture in a shaker at 28 °C and 120 rpm for 60 hours until the concentration of the bacterial liquid is stable, and obtain an Eurotium cristatum suspension, a Bacillus suspension, and a yeast suspension respectively.

[0479] Step 4: Solid fermentation

[0480] After evenly spraying the prepared citrus vegetable oil on the matrix; Mix the three liquid seed suspensions in a mass ratio of 4:0.8:0.2, and then evenly spray them onto the sterilized and cooled matrix (inoculation amount: 10% of the total amount of the solid matrix); Ensure that the matrix is loose and evenly distributed.

[0481] At a temperature of 28 °C; Maintain a relative humidity of 70%; Time: 7 days; Turn the matrix three times every morning, noon, and evening every day to ensure oxygen supply and uniform fermentation.

[0482] Step 5: Fermentation termination

[0483] After fermentation, high-speed centrifugation is carried out to obtain the fermented liquid and solid; the solid is added with 50% ethanol according to a mass ratio of 1:10, and ultrasonic extraction is carried out for 30 minutes; the supernatant is taken by centrifugation or filtration and mixed with the fermented liquid to obtain the soothing and repairing composition.

[0484] Among them, the Eurotium cristatum is Eurotium cristatum 2099, China Center for Industrial Culture Collection.

[0485] The Bacillus genus is Bacillus subtilis 10732, China Center for Industrial Culture Collection.

[0486] The yeast is Debaryomyces hansenii, 1266, China Center for Industrial Culture Collection.

[0487] This embodiment also provides a sensitive skin repair cream, and according to parts by weight, its preparation raw materials include:

[0488] Component A:

[0489] Stearic acid 1.5 parts

[0490] Glycerol monostearate 1 part

[0491] PEG-7 glyceryl cocoate 0.5 part

[0492] Tocopherol (vitamin E) 0.5 part

[0493] Cetearyl alcohol 2.5 parts

[0494] Ethoxystearic acid 0.7 part

[0495] Ethylhexyl palmitate 3 parts

[0496] Tris(ethylhexanoic acid) glyceride 5 parts

[0497] Squalane 2 parts

[0498] Polydimethylsiloxane 1 part

[0499] Tocopheryl acetate 1 part

[0500] Jojoba (SIMMONDSIA CHINENSIS) seed oil 0.5 part

[0501] Limnanthes alba seed oil 0.5 part

[0502] Component B:

[0503] 59.08 parts of water

[0504] 0.05 part of disodium EDTA

[0505] 0.2 part of allantoin

[0506] 1 part of betaine

[0507] 1 part of xylitol

[0508] 5 parts of glycerol

[0509] 0.1 part of carbomer

[0510] 0.5 part of acryloyldimethyltaurate / VP copolymer

[0511] 0.05 part of sodium hyaluronate

[0512] Component C

[0513] 3 parts of water

[0514] 0.3 part of arginine

[0515] Component D1

[0516] 5 parts of butanediol

[0517] 0.5 part of p - hydroxyacetophenone

[0518] Component D2

[0519] 0.5 part of 1,2 - hexanediol

[0520] 1 part of panthenol

[0521] 3 parts of the soothing and repairing composition provided in this example

[0522] 0.02 part of Pelargonium graveolens oil

[0523] 1. Ensure that the machines, containers, and equipment used are cleaned and disinfected; reserve some water to rinse the oil pan; phase C is pre - dissolved completely; phase D1 is pre - heated slightly (about 75 °C) and dissolved completely, then cooled for standby;

[0524] 2. Add phase A to the oil pan, heat to 82 °C and dissolve completely, keep the temperature constant for 15 minutes;

[0525] 3. Add phase B to the emulsifying pan, heat to 82 °C and dissolve completely, homogenize for 3 - 5 minutes to dissolve, keep the temperature constant for 15 minutes;

[0526] 4. Filter and pump phase A into the emulsifying pan, rinse the oil pan with the reserved water and add it to the emulsifying pan together; homogenize for 10 - 15 minutes, stir at a constant temperature for 20 minutes, and then cool while stirring;

[0527] 5. Cool down to about 70 °C, add the pre-dissolved Phase C, and stir until neutralization is complete; continue stirring and cooling down.

[0528] 6. Cool down to about 45 °C, add the pre-dissolved materials of Phase D1 and Phase D2 respectively, and stir to mix evenly.

[0529] 7. Cool down to about 38 °C, stop stirring, take a sample for inspection, and filter the product through a 200-mesh filter cloth after passing the inspection.

[0530] Performance Test

[0531] Test 1: Physical Shape Test: Place the compositions and products prepared in Examples 1-7 at 5 °C, 25 °C, and 40 °C for 90 days, and observe the product characteristics. The test results are shown in Table 1.

[0532] Table 1 Test Results of Performance Test 1

[0533] Example 5℃ 25℃ 40℃ Composition 1 Stratification Stratification Stratification Composition 2 Stratification Stratification Stratification Composition 3 Stratification Stratification Stratification Composition 4 Stratification Stratification Stratification Composition 5 No stratification Slight stratification Slight stratification Composition 6 No stratification No stratification Slight stratification Composition 7 Stratification Stratification Stratification Example 1 No stratification No stratification No stratification Example 2 No stratification No stratification No stratification Example 3 No stratification No stratification No stratification Example 4 No stratification No stratification No stratification Example 5 No stratification No stratification No stratification Example 6 No stratification No stratification No stratification Example 7 Stratification Slight stratification Stratification

[0534] Test 2: Repair Test

[0535] Difference in Transepidermal Water Loss Rate

[0536] 25 to 50-year-old healthy subjects (all subjects already have characteristics of sensitive skin, such as being prone to allergies, red blood streaks, etc.), 18 males and 38 females, with an average age of 30.2 ± 1.21 years.

[0537] Application Site: Forearm

[0538] Application Method: When applying on the forearm, the test sample is applied once at a dosage of (2.0 ± 0.1) mg / cm 2 and spread evenly in the test area using a latex finger cot.

[0539] Test Procedures

[0540] The inner part of the subject's arm should not be exposed to water before the visit.

[0541] Before the experiment, the subjects should clean their arms with dry paper towels uniformly.

[0542] Mark 2 measurement areas on the inner side of the subject's left or right arm, each area with an area of 3 cm * 3 cm, and the distance between the areas is at least 1 cm.

[0543] The sample area and the negative control area are randomly distributed in the calibrated area to ensure statistical balance of the position of each area: The subject exposes the arm and rests for 30 minutes in an environment with a temperature of 21±1°C and a relative humidity of 50±10% RH; Apply the inducer (0.1% histamine solution) to the sample area and the negative area for 10 minutes. After the application is completed and before using the sample (Timm), measure the transepidermal water loss rate of the calibrated area with a Tewameter and measure the heme of the calibrated area with a Mexamter;

[0544] Use the sample in the calibrated sample area according to the requirements of the sample usage method;

[0545] After using the sample, after the subject exposes the arm and rests for 1 hour (T1) in an environment with a temperature of 21±1°C and a relative humidity of 50±10% RH, measure the transepidermal water loss rate of the calibrated area with a Tewameter;

[0546] After 1 hour (T1), measure the heme of the calibrated area with a Mexamter;

[0547] Use the sample continuously for 1 month and stop using it for 1 week, and check the skin condition.

[0548] Difference in transepidermal water loss rate of the skin

[0549] The Courage+Khazaka transdermal water loss and water evaporation heat loss test probe Tewameter TM300 is used to detect the transepidermal water loss rate of the skin. The control group is water.

[0550] Table 3 Comparison of transepidermal water loss rate before and after

[0551]

[0552]

[0553] Difference in heme.

[0554] The Courage+Khazaka skin melanin and heme tester Mexamter MX18.

[0555] Table 4 Comparison of heme before and after

[0556]

[0557] Test 3: Human skin patch test

[0558] Materials and methods

[0559] 1. Test substances: Skin care products of Examples 1 to 7.

[0560] 2. Negative control: Blank control.

[0561] 3. Subjects: A total of 56 people, including 18 males and 38 females, aged 25 to 50 years old, with an average age of 30.2 ± 1.21 years old, meeting the volunteer inclusion criteria for subjects.

[0562] 4. Patch test method: Select qualified patch test equipment. Using the closed patch test method, place approximately 0.020 mL - 0.025 mL of the test substance in the patch tester, and apply it to the flexor side of the forearm of the subject with a low-sensitization tape. After 24 hours, remove the test substance, and observe the skin reaction at 0.5, 24, and 48 hours after removal. Record the results according to the skin reaction grading standard in the "Technical Specifications for Cosmetics Safety" (2015 Edition).

[0563] Table 4 Summary of Results of Human Skin Patch Tests

[0564]

[0565] Test 4: Contents of the Subject Questionnaire

[0566] The products prepared in Examples 1 - 7 were subjected to a skin care product questionnaire test. A total of 56 people were selected, including 18 males and 38 females, aged 25 to 50 years old, with an average age of 30.2 ± 1.21 years old, meeting the volunteer inclusion criteria for subjects. Among them, 42 subjects were confirmed to have allergic skin.

[0567] The questionnaire contents are shown in Table 5.

[0568] Table 5 Contents of the Subject Questionnaire

[0569] 5 points indicate full agreement, 4 points indicate relatively agreement, 3 points indicate neither agreement nor disagreement (i.e., average), 2 points indicate relatively disagreement, and 1 point indicates full disagreement. The results are the numerical aggregates of 42 people.

[0570] Table 6 Results of the Subject Questionnaire

[0571] Example Problem 1 Problem 2 Problem 3 Problem 4 Problem 5 Problem 6 1 208 207 210 209 200 201 2 208 207 210 208 193 193 3 209 206 208 200 161 179 4 207 206 209 201 152 174 5 208 207 209 208 187 186 6 209 208 208 198 150 174 7 184 186 207 195 182 168

[0572] Figure 1 Pictures when the product provided in Example 1 of the present invention was subjected to a repair test.

[0573] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that they can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements for some or all of the technical features; and these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A soothing and repairing composition, characterized in that: The composition comprises: scutellaria baicalensis root extract and alpine edelweiss extract.

2. A soothing and repairing composition according to claim 1, characterized in that: The mass ratio of the scutellaria root extract to the alpine edelweiss extract is 3-7:2-8.

3. A soothing and repairing composition according to claim 1, characterized in that: The mass ratio of the scutellaria root extract to the alpine edelweiss extract is 5:

4.

4. A soothing and repairing composition according to claim 1, characterized in that: The composition also includes citrus peel extract.

5. A soothing and repairing composition according to claim 4, characterized in that: The mass ratio of the citrus peel extract to the alpine edelweiss extract is 1:

1.

6. A soothing and repairing composition according to claim 4 or 5, characterized in that: The scutellaria root extract, alpine edelweiss extract and citrus peel extract are prepared by fermenting scutellaria root, alpine edelweiss and citrus peel with golden flower fungus.

7. A soothing and repairing composition according to claim 6, characterized in that: The bacterial species used in the fermentation also include Bacillus and / or yeast for symbiotic fermentation.

8. A soothing and repairing composition according to claim 7, characterized in that: The Bacillus species is Bacillus subtilis.

9. A soothing and repairing composition according to claim 7, characterized in that: The yeast is Debaryomyces hansenii.

10. A soothing and repairing composition according to any one of claims 1 to 7, characterized in that: Used in daily chemical field.

Images