Plant cell extract and cosmetic or medicine containing same

Abstract

The invention discloses a plant cell extract and a preparation method and application thereof, the plant cell extract is a purple perilla cell extract, the purple perilla cell extract mainly comprises perillaldehyde and a flavonoid substance, the mass ratio of perillaldehyde is 20%, the flavonoid substance mainly comprises quercetin, and the mass ratio of quercetin is 8%. Through combined application of the perilla cell extract and the anti-Nrf2 monoclonal antibody, the performance of the perilla cell extract and the anti-Nrf2 monoclonal antibody in cosmetics and medicines shows a relatively remarkable enhancement effect, especially in the aspects of aging resistance, oxidation resistance and immunoregulation. Therefore, combined application of the perilla cell extract and the anti-Nrf2 monoclonal antibody has wide prospects and potential, and the perilla cell extract and the anti-Nrf2 monoclonal antibody are suitable for anti-aging, skin care and treatment of immune-related diseases.

Description

Technical Field

[0001] The present invention relates to the application field of plant extracts, and particularly to a plant cell extract and its application in cosmetics or pharmaceuticals. Background Art

[0002] With the increasing demand for beauty and skin care, plant extracts are widely used in the research and development of cosmetics and pharmaceuticals due to their natural, non-toxic side effects. Existing plant extracts are mostly extracts of common external parts of plants, such as roots, stems, leaves, etc., and these extracts have certain limitations in terms of active ingredient content and extraction efficiency. In recent years, plant cell extracts, as a new type of natural extract, have gradually become a research hotspot due to their rich variety of active substances, stable sources and high extraction efficiency.

[0003] However, in the prior art, there are still the following problems in the research of plant cell extracts and their application in cosmetics and pharmaceuticals: the extraction methods mostly rely on traditional mechanical crushing and solvent extraction methods, with low extraction efficiency and inability to fully extract the effective components in plant cells; the types of active ingredients in plant cell extracts are relatively single, and the stability of the components is poor, making it difficult to ensure long-term effectiveness; existing plant cell extracts are mostly used in a single skin care field, lacking research and application of multi-functionalization.

[0004] Therefore, it has become an urgent problem to develop a plant cell extract that can efficiently extract active ingredients in plant cells and can be widely used in cosmetics and pharmaceuticals. Summary of the Invention

[0005] The purpose of the present invention is to provide a plant cell extract that can effectively extract active ingredients in plant cells and can be widely used in cosmetics or pharmaceuticals, and has obvious effects such as anti-aging, antioxidant, repairing skin barrier, and improving skin hydration.

[0006] To achieve the above purpose, the present invention provides the following technical solutions:

[0007] Therefore, on the one hand, the present invention discloses a plant cell extract, the plant cell extract is a perilla cell extract, the main components in the perilla cell extract include perillaldehyde and flavonoids, wherein the mass ratio of perillaldehyde is 20%, and the flavonoids are mainly quercetin, and its mass ratio is 8%.

[0008] On the one hand, the present invention also discloses a method for preparing the above-mentioned perilla cell extract, and the method includes the following steps:

[0009] (1) Perilla seed culture and cell proliferation;

[0010] (2) Preparation of perilla cell extract.

[0011] Preferably, the perilla seed cultivation and cell proliferation in step (1) of the present invention include the following steps:

[0012] (1) Disinfection of perilla seeds: Take out the perilla seeds, soak them in 0.1% mercuric chloride solution for 10 minutes to fully kill the surface bacteria and fungi; then rinse them repeatedly with sterile water 3 times to ensure the removal of residual disinfectant;

[0013] (2) Germination of perilla seeds: The disinfected seeds are placed in MS medium, supplemented with 1% sucrose, 0.1% IAA and 1.0 mg / L BAP; cultured in an environment of 25 °C, 16 hours of light and 8 hours of darkness for 7 days, and it is observed that the seeds begin to germinate and form initial plant buds;

[0014] (3) Perilla cell culture: Select the germinated plant buds for cell culture and transfer them to MS medium; adjust the pH value of the medium to 5.8, supplement 1% sucrose, place them in a culture environment of 25 °C, and maintain a cycle of 16 hours of light and 8 hours of darkness; change the medium every 3 days, and the cell density gradually increases.

[0015] Preferably, the preparation of perilla cell extract in step (2) of the present invention includes the following steps:

[0016] (1) Collection of perilla cells: After the cell culture is completed, centrifuge at 3000 rpm for 10 minutes to collect the cells. The collected cells are washed twice with sterile PBS buffer to remove impurities in the medium. The washed cell precipitate is immediately used for the subsequent cell disruption step;

[0017] (2) Disruption of perilla cells: Add the collected cell precipitate to liquid nitrogen, freeze it and then disrupt it; use an ultrasonic disruptor during the disruption process, set the ultrasonic power to 30%, oscillate for 60 seconds each time, and repeat 3 times to ensure the complete release of cell contents; after ultrasonic disruption, centrifuge at 5000 rpm for 10 minutes to remove cell debris, collect the supernatant, and proceed to the next extraction step;

[0018] (3) Preparation of perilla cell extraction solution: Mix the supernatant after ultrasonic disruption with 0.1% ethanol solution at a ratio of 1 g of cells: 10 mL of solvent, stir at 4 °C for 12 hours to ensure the effective dissolution of active ingredients in the cells; after the extract is centrifuged at 5000 rpm for 10 minutes to remove residual cell debris, filter it using a 0.22 μm filter membrane to obtain a crude extract;

[0019] (4) Concentration of the perilla cell extract: The crude extract is processed by low-temperature vacuum concentration to be concentrated to 1 / 5 of the original volume; finally, the concentrated extract is frozen and lyophilized to obtain the powder of the perilla cell extract.

[0020] In one aspect, the present invention also discloses a cosmetic, which is an anti-aging cream, and the formula of the anti-aging cream is as follows according to the mass ratio:

[0021] (1) Perilla cell extract: 5%;

[0022] (2) Anti-Nrf2 monoclonal antibody: 50 μg / mL;

[0023] (3) Glycerol: 5%;

[0024] (4) Aqueous phase: Deionized water, supplemented to 100%.

[0025] Preferably, the amino acid sequences of the heavy chain variable region and the light chain variable region of the anti-Nrf2 monoclonal antibody of the present invention are respectively shown as SEQ ID NO.1 and SEQ ID NO.2.

[0026] In one aspect, the present invention also discloses a medicine, which is an oral drug, and the formula of the oral drug is as follows according to the mass ratio:

[0027] (1) Perilla cell extract: 3%;

[0028] (2) Anti-Nrf2 monoclonal antibody: 20 μg / mL;

[0029] (3) Lactose: 20%;

[0030] (4) Plant fiber: 10%;

[0031] (5) Aqueous phase: Deionized water, supplemented to 100%.

[0032] In one aspect, the present invention also discloses the application of the perilla cell extract in the preparation of cosmetics and medicines.

[0033] In one aspect, the present invention also discloses the application of the anti-Nrf2 monoclonal antibody in the preparation of cosmetics and medicines.

[0034] By culturing perilla cells and extracting cell extracts therefrom, the present invention not only greatly improves the yield of plant cell extracts, but also can effectively retain the active ingredients in plant cells. In addition, the obtained plant cell extracts have strong antioxidant, anti-aging, moisturizing, repair and other effects, and can be widely used in the fields of cosmetics and pharmaceuticals, with significant commercial value. At the same time, the combined application of perilla cell extracts and anti-Nrf2 monoclonal antibodies can achieve better application effects, which expands the application of perilla cell extracts and anti-Nrf2 monoclonal antibodies in the fields of cosmetics and pharmaceuticals.

[0035] Perilla is a traditional Chinese medicinal plant. Perilla contains rich polyphenols, flavonoids, volatile oils, fatty acids and various trace elements, and it has the following effects:

[0036] (1) Deep repair and regeneration: Perilla contains rich omega-3 fatty acids, and these components have the potential in skin cell repair, promoting collagen synthesis and anti-aging. By extracting the active ingredients from perilla cell cultures, these fatty acids can provide a strong skin regeneration effect, repair damaged skin, and improve the skin barrier function, especially showing unexpected effects in the care of sensitive skin or damaged skin.

[0037] (2) Anti-inflammatory and sedative effects: Volatile components of perilla such as perillaldehyde and α-perillene are known to have anti-inflammatory effects. The cell extracts concentrate these components and can play a powerful anti-inflammatory effect in skin care products, helping to relieve skin redness, allergies and other discomforts caused by environmental stimuli or chemical components.

[0038] (3) Anti-ultraviolet and anti-photoaging: Perilla contains rich antioxidant substances (such as flavonoids and phenolic acids), which can help resist ultraviolet damage and inhibit skin aging (such as wrinkles and age spots) caused by ultraviolet irradiation. The concentration of plant cell extracts after cell culture can show an unexpected ultraviolet barrier effect, providing additional protection for daily skin care products.

[0039] (4) Skin microecological balance: Perilla has certain antibacterial activity, and the components in plant cell extracts may have a balancing effect on the skin surface microbial community (such as skin bacteria), helping to maintain the health of the skin microecology, reducing the growth of harmful bacteria, and thus reducing skin inflammation, acne and other problems. Description of the Drawings

[0040] Figure 1 SDS-PAGE detection results of anti-Nrf2 monoclonal antibody, where 1 is anti-Nrf2 monoclonal antibody. Detailed Embodiments

[0041] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the technical field to which this invention belongs. The terms used in the description of the present invention herein are for the purpose of describing specific embodiments only and are not intended to limit the present invention. The term "and / or" as used herein includes any and all combinations of one or more of the related listed items.

[0042] Unless otherwise specified, the reagents, methods, and equipment used in the present invention are conventional reagents, methods, and equipment in this technical field. Unless otherwise specified, the reagents and materials used in the following examples are all commercially available.

[0043] Example 1: Preparation and Testing of Perilla Cell Extract

[0044] I. Perilla Seed Cultivation and Cell Proliferation

[0045] 1. Disinfection of perilla seeds: Take out the perilla seeds and soak them in 0.1% mercuric chloride solution for 10 minutes to fully kill the surface bacteria and fungi; then rinse them with sterile water repeatedly for 3 times to ensure the removal of residual disinfectant.

[0046] 2. Germination of perilla seeds: The disinfected seeds are placed in MS medium supplemented with 1% sucrose, 0.1% IAA (auxin), and 1.0 mg / L BAP (cytokinin); cultured in an environment of 25°C, 16 hours of light, and 8 hours of darkness for 7 days, and it is observed that the seeds begin to germinate and form initial plant buds.

[0047] 3. Perilla cell culture: Select the germinated plant buds for cell culture and transfer them to MS medium. The pH value of the medium is adjusted to 5.8, and 1% sucrose is supplemented. Place it in a culture environment of 25°C, maintaining a cycle of 16 hours of light and 8 hours of darkness. Replace the medium every 3 days, and the cell density gradually increases. The specific cell culture data is shown in Table 1. During the experiment, it is observed that the cell proliferation gradually increases, and the cell density reaches 8×10 6 cells / mL after 20 days, indicating that perilla cells can continuously proliferate under suitable culture conditions.

[0048] Table 1 Results of Cell Proliferation Experiment

[0049]

[0050] II. Preparation of Perilla Cell Extract

[0051] 1. Collection of perilla cells: After the cell culture is completed, centrifuge at 3000 rpm for 10 minutes to collect the cells. The collected cells are washed twice with sterile PBS buffer to remove impurities in the medium. The washed cell precipitate is immediately used for the subsequent cell disruption step.

[0052] 2. Perilla cell disruption: Add the collected cell precipitate into liquid nitrogen, and perform disruption after freezing. During the disruption process, use an ultrasonic disruptor, set the ultrasonic power to 30%, oscillate for 60 seconds each time, and repeat 3 times to ensure the complete release of cell contents. After ultrasonic disruption, centrifuge at 5000 rpm for 10 minutes to remove cell debris, collect the supernatant, and proceed to the next extraction step.

[0053] 3. Preparation of perilla cell extraction solution: Mix the supernatant after ultrasonic disruption with 0.1% ethanol solution at a ratio of 1 g of cells: 10 mL of solvent, place it at 4°C and stir for 12 hours to ensure the effective dissolution of active ingredients inside the cells. After the extract is centrifuged at 5000 rpm for 10 minutes to remove residual cell debris, filter it using a 0.22 μm filter membrane to obtain a crude extract.

[0054] 4. Concentration of perilla cell extract: Treat the crude extract by low-temperature vacuum concentration method, concentrate it to 1 / 5 of the original volume. Finally, freeze the concentrated extract and perform lyophilization to obtain the powder of perilla cell extract.

[0055] III. Analysis of Active Ingredients in Perilla Cell Extract

[0056] 1. High-performance liquid chromatography (HPLC) analysis: Use a C18 chromatographic column, with the mobile phase being water: acetonitrile (90:10), and set the flow rate to 1 mL / min. Perform HPLC analysis on the perilla cell extract to detect its main active ingredients. The analysis results show that the main components in the perilla cell extract include perillaldehyde and flavonoids. Among them, the mass ratio of perillaldehyde is 20%, and the flavonoid is mainly quercetin, with a mass ratio of 8%.

[0057] 2. DPPH radical scavenging ability: Use the DPPH radical scavenging experiment to detect the antioxidant ability of the perilla cell extract. Take perilla cell extracts with different concentrations (10 μg / mL, 20 μg / mL, 50 μg / mL), mix them with DPPH solution, incubate for 30 minutes, and measure the change in absorbance. The results show (Table 2) that the perilla cell extract has strong antioxidant ability, and its IC 50 is 45.6 μg / mL; as the concentration of the perilla cell extract increases, the DPPH radical scavenging rate increases significantly. At a concentration of 50 μg / mL, the DPPH radical scavenging rate reaches 85%.

[0058] Table 2 Results of DPPH Radical Scavenging Experiment

[0059]

[0060] 3. Anti-aging experiment on skin fibroblasts: Human skin fibroblasts (HFF-1) were selected as the model and treated with perilla cell extracts at different concentrations (10 μg / mL, 20 μg / mL, 50 μg / mL). After culturing for 48 hours, the synthesis of collagen and cell apoptosis were detected. The experimental data showed (Table 3) that the perilla cell extract could significantly promote collagen synthesis, and the collagen synthesis in the 50 μg / mL treatment group increased by about 60%; at the same time, it could also effectively reduce cell apoptosis, and the apoptosis rate in the 50 μg / mL treatment group decreased to 40%. In the skin fibroblast experiment, the perilla cell extract could significantly promote collagen synthesis and reduce cell apoptosis, showing its anti-aging effect.

[0061] Table 3 Results of skin fibroblast experiment

[0062]

[0063] 4. Inflammatory factor inhibition experiment: In HFF-1 cells stimulated with LPS (1 μg / mL), perilla cell extracts (10 μg / mL, 20 μg / mL, 50 μg / mL) were added and cultured for 48 hours. The concentrations of IL-6 and TNF-α were measured by ELISA. The results showed (Table 4) that the perilla cell extract could significantly inhibit the LPS-induced inflammatory response. The 10 μg / mL, 20 μg / mL, and 50 μg / mL treatment groups inhibited the secretion of IL-6 by about 40%, 60%, and 70% respectively; inhibited the secretion of TNF-α by about 30%, 50%, and 60%; the perilla cell extract could significantly inhibit the release of the inflammatory factors IL-6 and TNF-α induced by LPS at different concentrations, showing a good anti-inflammatory effect.

[0064] Table 4 Results of inflammatory factor inhibition experiment

[0065]

[0066] Example 2: Preparation and testing of anti-Nrf2 monoclonal antibody

[0067] 1. Animal immunization: Healthy BALB / c mice at 6-8 weeks old were selected. Recombinant Nrf2 protein (ab202153) was used as the immunogen and combined with complete Freund's adjuvant for the first immunization. The second immunization was carried out 2 weeks after the first immunization (using incomplete Freund's adjuvant), and then booster immunizations were carried out 2-3 times every 3 weeks. 3 weeks after immunization, the level of Nrf2-specific antibody in the mouse serum was detected by ELISA to screen the mouse with the strongest immune response.

[0068] 2. Spleen cell fusion: Select the mouse with the strongest immune response and collect spleen cells. The spleen cells are fused with anti-mouse lymphoma cells (SP2 / 0) at a ratio of 10:1, using polyvinyl alcohol (PEG) as the fusogen. The fused cells are inoculated into HAT medium and selectively cultured for 7 - 10 days to remove unfused cells.

[0069] 3. Clone screening and amplification: The fused cells are divided into monoclonal cells by the limiting dilution method, and clones that can secrete specific anti-Nrf2 antibodies are screened. The anti-Nrf2 antibody levels secreted by each clone are detected by ELISA, and high-efficiency monoclonal clones are selected for amplification.

[0070] 4. Monoclonal antibody preparation: The screened monoclonal cells are inoculated into RPMI1640 medium containing 10% fetal bovine serum and cultured for 10 - 14 days, and the supernatant is collected. Antibody purification is performed using a Protein A column. The purified monoclonal antibody is detected by SDS-PAGE, and the results show ( Figure 1 ) that the monoclonal antibody has good purity, and its purity can reach over 90%.

[0071] 5. Verification and comparison: Using surface plasmon resonance (SPR) technology for testing, the binding affinity (Kd value) of the anti-Nrf2 monoclonal antibody prepared in the present invention with Nrf2 is 1.2×10 -9 M. While the affinity (Kd value) of the commercial antibody (MCE, catalog number: HY-P81051) is 5.5×10 -9 M, which is lower than that of the self-made monoclonal antibody, showing a weaker binding ability.

[0072] 6. Sequence detection: Extract the mRNA of the hybridoma cells of the anti-Nrf2 monoclonal antibody and analyze the variable region sequence of the anti-Nrf2 monoclonal antibody therefrom. The amino acid sequences of the heavy chain variable region and the light chain variable region of the anti-Nrf2 monoclonal antibody are shown as SEQ ID NO.1 and SEQ ID NO.2 respectively.

[0073] Example 3: Application of plant cell extract

[0074] I. Application in cosmetics

[0075] 1. Formulation design: In this experiment, perilla cell extract is used as the main active ingredient to formulate an anti-aging cream. At the same time, the anti-Nrf2 monoclonal antibody is added to the formulation to enhance the antioxidant and anti-aging effects.

[0076] (1) Group A (perilla cell extract + anti-Nrf2 monoclonal antibody group), all are mass ratios:

[0077] 1) Perilla cell extract: 5%;

[0078] 2) Anti-Nrf2 monoclonal antibody: 50 μg / mL;

[0079] 3) Glycerol: 5%;

[0080] 4) Aqueous phase: Deionized water, supplemented to 100%.

[0081] (2) Group B (Perilla cell extract group), all by mass ratio:

[0082] 1) Perilla cell extract: 5%;

[0083] 2) Glycerol: 5%;

[0084] 3) Aqueous phase: Deionized water, supplemented to 100%.

[0085] (3) Group C (Commercial anti-aging cream group): Proya Ruby Cream.

[0086] 2. Experimental period: 8 weeks. Observe the skin effects and evaluate using indicators such as skin moisture, elasticity, and wrinkle depth.

[0087] 3. The experimental results (results after 8 weeks) are shown in Table 5. The combined use of perilla cell extract and anti-Nrf2 monoclonal antibody significantly improved skin hydration, elasticity, and glossiness, and reduced wrinkle depth. The effect was better than that of the group using perilla cell extract alone. At the same time, the effects of the group using perilla cell extract alone and the combined application of perilla extract and anti-Nrf2 monoclonal antibody were both better than those of the commercial anti-aging cream.

[0088] Table 5 Summary of experimental data

[0089]

[0090] II. Application of perilla cell extract in drugs

[0091] 1. Formulation design: Perilla cell extract is used in an oral drug formulation and combined with anti-Nrf2 monoclonal antibody to evaluate its antioxidant and immunomodulatory effects.

[0092] (1) Group A (Perilla cell extract + anti-Nrf2 monoclonal antibody group), all by mass ratio:

[0093] 1) Perilla cell extract: 3%;

[0094] 2) Anti-Nrf2 monoclonal antibody: 20 μg / mL;

[0095] 3) Lactose: 20%;

[0096] 4) Plant fiber: 10%;

[0097] 5) Aqueous phase: Deionized water, supplemented to 100%.

[0098] (2) Group B (Perilla cell extract group), all by mass ratio:

[0099] 1) Perilla cell extract: 3%;

[0100] 2) Lactose: 20%;

[0101] 3) Plant fiber: 10%;

[0102] 4) Aqueous phase: Deionized water, supplemented to 100%.

[0103] 2. Experimental period: 6 weeks, observing the antioxidant capacity and immune regulation effect of the orally administered drug.

[0104] 3. The experimental data (results after 6 weeks) are shown in Table 6. The combined use of perilla cell extract and anti-Nrf2 monoclonal antibody significantly increased the activities of antioxidant enzymes (SOD, CAT) and more effectively inhibited the release of immune inflammatory factors (IL-6, TNF-α). This indicates that the combined use of anti-Nrf2 monoclonal antibody and perilla cell extract has a synergistic effect in immune regulation and antioxidant, enhancing its therapeutic potential.

[0105] Table 6 Summary of experimental data

[0106]

[0107] III. Summary

[0108] 1. Application in cosmetics: The combined use of perilla cell extract and anti-Nrf2 monoclonal antibody has shown significant effects in skin anti-aging, especially in skin moisture, elasticity, wrinkle depth and glossiness, which are superior to the control group using only perilla cell extract. This proves that anti-Nrf2 monoclonal antibody can enhance the anti-aging and antioxidant effects of perilla cell extract.

[0109] 2. Application in drugs: In the experiment of orally administered drugs, the combined use of perilla cell extract and anti-Nrf2 monoclonal antibody can significantly increase the activities of antioxidant enzymes (SOD, CAT) and effectively inhibit the levels of inflammatory factors (IL-6, TNF-α). Compared with the control group using only perilla cell extract, the combined use group shows stronger immune regulation and antioxidant capabilities.

[0110] 3. Conclusion: The combined application of perilla cell extract and anti-Nrf2 monoclonal antibody shows relatively significant enhancement effects in both cosmetics and drugs, especially in anti-aging, antioxidant and immune regulation. Therefore, the combined application of perilla cell extract and anti-Nrf2 monoclonal antibody has broad prospects and potential, and is applicable to the treatment of anti-aging, skin care and immune-related diseases.

[0111] The above embodiments are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications made without departing from the spirit and principle of the present invention shall be equivalent replacement methods and are all included in the protection scope of the present invention.

Claims

1. A plant cell extract, characterized in that The plant cell extract is a perilla cell extract, and the main components of the perilla cell extract include perilla aldehyde and flavonoids, wherein the mass ratio of perilla aldehyde is 20%, and the flavonoids are mainly quercetin, and the mass ratio of quercetin is 8%.

2. A method for preparing the perilla cell extract according to claim 1, characterized in that: The method comprises the following steps: (1) Perilla seed culture and cell proliferation; (2) Preparation of Perilla cell extract.

3. The method according to claim 2, characterized in that The step (1) of perilla seed culture and cell proliferation comprises the following steps: (1) Disinfection of Perilla seeds: Take out the Perilla seeds and soak them in 0.1% mercuric chloride solution for 10 minutes to fully kill surface bacteria and fungi; then rinse them repeatedly with sterile water for 3 times to ensure that the residual disinfectant is removed; (2) Germination of Perilla seeds: The sterilized seeds were placed in MS medium supplemented with 1% sucrose, 0.1% IAA, and 1.0 mg / L BAP; they were cultured at 25°C, 16 h light, and 8 h dark for 7 days, and the seeds began to germinate and form early plant buds; (3) Perilla cell culture: Select germinated plant buds for cell culture and transfer them to MS medium; adjust the pH value of the medium to 5.8, supplement with 1% sucrose, place in a culture environment at 25°C, maintain a 16-hour light and 8-hour dark cycle; replace the medium every 3 days, and gradually increase the cell density.

4. The method according to claim 2, characterized in that: The preparation of the perilla cell extract in step (2) comprises the following steps: (1) Collection of Perilla cells: After cell culture, the cells were collected by centrifugation at 3000 rpm for 10 minutes. The collected cells were washed twice with sterile PBS buffer to remove impurities in the culture medium; the cell pellet after washing was immediately used in the subsequent cell disruption step; (2) Perilla cell disruption: The collected cell pellets were added to liquid nitrogen, frozen and then disrupted; an ultrasonic disruptor was used during the disruption process, with the ultrasonic power set at 30%, and each oscillation was performed for 60 seconds, repeated 3 times to ensure that the cell contents were completely released; after ultrasonic disruption, the cells were centrifuged at 5000 rpm for 10 minutes to remove cell debris, and the supernatant was collected for the next step of extraction; (3) Preparation of perilla cell extract solution: The supernatant after ultrasonic disruption was mixed with 0.1% ethanol solution at a ratio of 1 g cell: 10 mL solvent, and stirred at 4°C for 12 hours to ensure effective dissolution of the active ingredients in the cells; the extract was centrifuged at 5000 rpm for 10 minutes to remove residual cell debris, and then filtered using a 0.22 μm filter membrane to obtain a crude extract; (4) Concentration of the perilla cell extract: The crude extract is concentrated to 1 / 5 of its original volume by low-temperature vacuum concentration; finally, the concentrated extract is frozen and freeze-dried to obtain a powder of the perilla cell extract.

5. A cosmetic, characterized in that: The cosmetic is an anti-aging cream, wherein the formula of the anti-aging cream is as follows according to the mass ratio: (1) the perilla cell extract of claim 1: 5%; (2) Anti-Nrf2 monoclonal antibody: 50 μg / mL; (3) Glycerol: 5%; (4) Water phase: deionized water, replenished to 100%.

6. The cosmetic according to claim 5, characterized in that: The amino acid sequences of the heavy chain variable region and the light chain variable region of the anti-Nrf2 monoclonal antibody are shown in SEQ ID NO.1 and SEQ ID NO.2, respectively.

7. A medicine, characterized in that: The medicine is an oral medicine, wherein the formula of the oral medicine is as follows according to the mass ratio: (1) the perilla cell extract of claim 1: 3%; (2) the anti-Nrf2 monoclonal antibody of claim 6: 20 μg / mL; (3) Lactose: 20%; (4) Plant fiber: 10%; (5) Water phase: deionized water, replenished to 100%.

8. Use of the perilla cell extract according to claim 1 in the preparation of cosmetics and medicines.

9. Use of the anti-Nrf2 monoclonal antibody according to claim 6 in the preparation of cosmetics and medicines.

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