Enzymolysis device for regulating and controlling histone acetylation through targeted SIRT1

Abstract

The invention discloses an enzymolysis device for regulating and controlling histone acetylation through targeted SIRT1, relates to the technical field of cell enzymolysis, and particularly relates to an enzymolysis device for regulating and controlling histone acetylation through targeted SIRT1. In the process of carrying out enzymolysis operation on cells by using the enzymolysis device, the nano-carrier loaded with the enzymolysis regulation and control assembly needs to be introduced into a living body through intravenous injection, and in the injection process, through mutual cooperation of the processing assembly, the liquid supply assembly, the rotating assembly, the driving assembly and other components, the enzymolysis regulation and control assembly can be adjusted and controlled; the PH value and the ionic strength of the magnetic nano-carrier are adjusted, the appropriate PH value can maintain the stability of surface charges of the nano-carrier, electrostatic repulsive force is generated between the nano-carriers, meanwhile, the buffer solution can adjust the ionic strength, double-electrode-layer compression caused by high ionic strength is avoided, and the stability of the surface charges of the nano-carrier is improved. Therefore, electrostatic repulsive force formed by charges on the surface of the nano-carrier is ensured to play a role effectively, and the magnetic nano-carrier is prevented from being agglomerated during injection and conveying.

Description

Technical Field

[0001] The present invention relates to the technical field of cell enzymolysis, and specifically to an enzymolysis device for regulating histone acetylation by targeting SIRT1. Background Art

[0002] During the process of using an enzymolysis device to perform enzymolysis operation on cells, a nanocarrier loaded with an enzymolysis regulation component is intravenously injected into an organism. Through targeted delivery in vivo, the nanocarrier specifically recognizes and binds to cells containing SIRT1. Once reaching the target position, the histone acetylation detection and feedback module starts to monitor the histone acetylation level in real time and transmits the signal to the control system. According to the feedback signal, the control system activates the energy supply and drive system to regulate the release or activity of the SIRT1 regulator, so as to achieve precise regulation of the histone acetylation level. For example, if an increase in the acetylation level is detected, the control system drives the SIRT1 activator to be released from the nanocarrier and act on SIRT1 to promote its deacetylation effect on histones; if the acetylation level decreases, the relevant components are adjusted to inhibit the activity of SIRT1 or promote the supply of its substrates to restore the acetylation level to normal.

[0003] To improve the targeting effect of the nanocarrier in vivo, the nanocarrier is combined with a magnetic material (such as superparamagnetic iron oxide nanoparticles) to endow it with magnetic response ability. Under the guidance of an external magnetic field, the magnetic nanocarrier can move more precisely and directionally to the target cells. During the intravenous injection of the magnetic nanocarrier, in the injection pipeline, due to the action of magnetic force and hydrodynamic factors, the nanocarrier may agglomerate due to local flow rate changes, adsorption on the pipe wall, and the interaction of collisions. This will not only affect the distribution and targeting effect of the nanocarrier in vivo, but may also cause blood vessel blockage. Therefore, we propose an enzymolysis device for regulating histone acetylation by targeting SIRT1. Summary of the Invention

[0004] The purpose of the present invention is to provide an enzymolysis device for regulating histone acetylation by targeting SIRT1 to solve the problems raised in the above background art.

[0005] To achieve the above purpose, the present invention provides the following technical solution: An enzymolysis device for regulating histone acetylation by targeting SIRT1, including an operating frame for transporting the nanocarrier, wherein the surface of the nanocarrier is modified with a ligand that specifically recognizes and binds to the SIRT cell surface receptor, and a magnetic material for assisting targeted positioning is arranged inside the nanocarrier. Further includes: An L-shaped piston tube arranged on the operating frame for supplying and transporting the nanocarrier, and a processing component arranged on the operating frame for preventing the agglomeration of the nanocarrier transported inside the L-shaped piston tube; The processing component includes a mixing cylinder fixed on an operation frame. One end of an L-shaped piston tube communicates with the inside of the mixing cylinder. The front end of the mixing cylinder is connected to a syringe tube in a communicating manner. The rear end of the mixing cylinder is rotatably connected to a liquid storage cylinder through a rotating component. A liquid supply component for supplying liquid to the inside of the mixing cylinder is arranged on the liquid storage cylinder. A driving component for driving the liquid storage cylinder is arranged on the mixing cylinder. The end of the liquid storage cylinder is in a communicating state and is rotatably connected to a piston cylinder. A connecting component for connecting the piston cylinder is arranged on the operation frame. A buffer solution for maintaining the pH value and ionic strength of the nano-carrier is stored inside the piston cylinder. An extrusion component for assisting liquid discharge is arranged on the L-shaped piston tube and the piston cylinder.

[0006] Preferably, multiple groups of the liquid supply components are arranged on the liquid storage cylinder, and the multiple groups of liquid supply components are arranged in an annular array. The liquid supply component includes a liquid outlet pipe fixed on the liquid storage cylinder. The two ends of the liquid outlet pipe communicate with the inside of the liquid storage cylinder and the mixing cylinder respectively. One end of the liquid outlet pipe located inside the mixing cylinder is connected to an L-shaped pipe through a bellows pipe. A telescopic component for telescopically connecting the L-shaped pipe is arranged on one side of the liquid storage cylinder. A pushing component for pushing the L-shaped pipe is arranged inside the mixing cylinder.

[0007] Preferably, the telescopic component includes a mounting ring sleeved outside the L-shaped pipe. An L-shaped frame is fixed on the mounting ring. Multiple sets of sleeves are fixed on the L-shaped frame. A sliding rod is slidably connected to the sleeve. One end of the sliding rod is fixed with a connecting plate, and the connecting plate is fixed to the front end of the liquid storage cylinder. A first spring is sleeved outside the sleeve, and the two ends of the first spring are connected to the L-shaped frame and the connecting plate respectively.

[0008] Preferably, the pushing component includes a transmission pin fixed on the L-shaped frame. Multiple spherical protrusions for abutting and transmitting with the end of the transmission pin are fixed in an annular array inside the mixing cylinder.

[0009] Preferably, the rotating component includes a mounting hole opened at the end of the mixing cylinder. An annular groove is opened inside the mounting hole. An annular plate is rotatably connected inside the annular groove. The annular plate is sleeved and fixed outside the liquid storage cylinder. The cross sections of the annular groove and the annular plate are arranged in a T shape.

[0010] Preferably, the driving component includes a gear ring sleeved and fixed outside the liquid storage cylinder. A fixed frame is fixed outside the mixing cylinder. A rotating shaft is rotatably connected to the fixed frame. A gear is fixed on the rotating shaft. The gear and the gear ring are meshed with each other. A driving motor for driving the rotating shaft is installed on the fixed frame.

[0011] Preferably, the connection component includes a connection frame fixed to the operation frame, and a fixing plate for connecting and fixing the piston cylinder is fixed between the connection frame and the outer side of the piston cylinder.

[0012] Preferably, the extrusion component includes a piston plate slidably connected inside the piston cylinder and the L-shaped piston tube. An extrusion rod is fixed on the piston plate, and a transmission component for driving the extrusion rod is arranged on the connection frame.

[0013] Preferably, the transmission component includes a transmission plate. One ends of two groups of the extrusion rods are fixed to the transmission plate. A rectangular plate is fixed on one side of the transmission plate. A support frame is fixed on the connection frame, and a cylinder for driving the rectangular plate is installed on the support frame.

[0014] Compared with the prior art, the beneficial effects of the present invention are as follows: During the process of using the enzymatic hydrolysis device to perform enzymatic hydrolysis on cells in the present invention, it is necessary to introduce the nano-carrier loaded with the enzymatic hydrolysis regulation component into the organism by intravenous injection. During the injection process, through the mutual cooperation of components such as the processing component, the liquid supply component, the rotation component, and the driving component, the pH value and ionic strength of the magnetic nano-carrier are adjusted. The appropriate pH value can maintain the stability of the surface charge of the nano-carrier, causing electrostatic repulsion between the nano-carriers. At the same time, the buffer solution can adjust the ionic strength to avoid the compression of the double electric layer caused by high ionic strength, thereby ensuring that the electrostatic repulsion formed by the surface charge of the nano-carrier effectively plays a role and preventing the aggregation of the magnetic nano-carrier during injection and transportation. Description of the Drawings

[0015] Figure 1 is a schematic diagram of the overall external structure of the present invention; Figure 2 is a schematic diagram of the positional relationship between the extrusion component and the transmission component of the present invention; Figure 3 is a schematic diagram of the structure of the transmission component of the present invention; Figure 4 is a schematic diagram of the structure of the extrusion component of the present invention; Figure 5 is a schematic diagram of the structure of the driving component of the present invention; Figure 6 is a schematic diagram of the structure of the rotation component of the present invention; Figure 7 is a schematic diagram of the structures of the liquid supply component, the telescopic component, and the pushing component of the present invention; Figure 8 is a schematic diagram of the state of each group of L-shaped tubes before being pushed in the present invention; Figure 9 is a schematic diagram of the state of each group of L-shaped tubes after being pushed in the present invention.

[0016] In the figure: 1 - operating frame; 2 - L-shaped piston tube; 301 - mixing cylinder; 302 - syringe tube; 303 - liquid storage cylinder; 304 - piston cylinder; 401 - mounting hole; 402 - annular groove; 403 - annular plate; 501 - gear ring; 502 - fixing frame; 503 - rotating shaft; 504 - gear; 505 - driving motor; 601 - liquid outlet pipe; 602 - L-shaped pipe; 603 - bellows pipe; 701 - mounting ring; 702 - L-shaped frame; 703 - sleeve; 704 - sliding rod; 705 - connecting plate; 706 - first spring; 801 - transmission pin; 802 - spherical protrusion; 901 - connecting frame; 902 - fixing plate; 1001 - piston plate; 1002 - extrusion rod; 1101 - transmission plate; 1102 - rectangular plate; 1103 - support frame; 1104 - air cylinder. Detailed implementation manners

[0017] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0018] Example 1, please refer to Figures 1-9 , an enzymatic device for regulating histone acetylation by targeting SIRT1 shown in the figure, including an operating frame 1 for delivering a nanocarrier, and a ligand specifically recognizing and binding to the SIRT1 cell surface receptor is modified on the surface of the nanocarrier; It should be noted here that: the ligand is a peptide ligand. Some short peptides can be designed to specifically recognize the SIRT1 cell surface receptor. These peptides are short chains composed of amino acids, and their amino acid sequences can be designed according to the binding sites of the target receptor. For example, by analyzing the structure of the SIRT1 receptor binding domain, the key amino acid sequence interacting with it is determined, and then a complementary peptide sequence is synthesized; A magnetic material for assisting targeted positioning is arranged inside the nanocarrier, and further includes: An L-shaped piston tube 2 for supplying and delivering the nanocarrier is arranged on the operating frame 1, and a processing assembly for preventing the agglomeration of the nanocarrier conveyed inside the L-shaped piston tube 2 is arranged on the operating frame 1; The processing component includes a mixing cylinder 301 fixed on the operating frame 1. One end of the L-shaped piston tube 2 communicates with the inside of the mixing cylinder 301. The front end of the mixing cylinder 301 is connected to a syringe 302 in a communicating manner. The rear end of the mixing cylinder 301 is rotatably connected to a liquid storage cylinder 303 through a rotating component. A liquid supply component for supplying liquid to the inside of the mixing cylinder 301 is provided on the liquid storage cylinder 303. A driving component for driving the liquid storage cylinder 303 is provided on the mixing cylinder 301. The end of the liquid storage cylinder 303 is in a communicating state and is rotatably connected to a piston cylinder 304. A connecting component for connecting the piston cylinder 304 is provided on the operating frame 1. A buffer solution for maintaining the pH value and ionic strength of the nanocarrier is stored inside the piston cylinder 304. An extrusion component for assisting liquid discharge is provided on the L-shaped piston tube 2 and the piston cylinder 304; It should be noted here that: during the process of enzymatically digesting cells using an enzymatic digestion device, it is necessary to intravenously inject a nanocarrier loaded with an enzymatic digestion regulation component into an organism. During the injection process, through the mutual cooperation of components such as the processing component, liquid supply component, rotating component, and driving component, the pH value and ionic strength of the magnetic nanocarrier are adjusted. An appropriate pH value can maintain the stability of the surface charge of the nanocarrier, causing electrostatic repulsion between the nanocarriers. At the same time, the buffer solution can adjust the ionic strength, avoiding the compression of the double electric layer caused by high ionic strength, thereby ensuring that the electrostatic repulsion formed by the surface charge of the nanocarrier effectively plays a role and preventing the aggregation of magnetic nanocarriers during injection and transportation; It is worth noting here that: the enzymatic digestion regulation component of the nanocarrier includes enzymes, enzyme inhibitors, or other regulatory factors that can specifically act on the SIRT1 pathway; Magnetic nanocarriers usually have magnetic materials added during their preparation process, such as iron oxide nanoparticles. These magnetic materials enable the nanocarriers to respond to an external magnetic field. After being injected into an organism, by applying a magnetic field at the target site, the nanocarriers are guided to specific tissue or cell regions, further enhancing the targeting property and facilitating the enzymatic digestion regulation component to play a role at the required position; The buffer solution can be a phosphate buffer solution, which is composed of sodium dihydrogen phosphate and disodium hydrogen phosphate. Its pH value is usually between 7.2 - 7.4, which is close to the physiological pH value of the organism. During the injection of magnetic nanocarriers, the phosphate buffer solution can provide a stable ionic environment, maintain the surface charge state of the nanoparticles, prevent aggregation caused by charge changes, and at the same time, the ionic components in the phosphate buffer solution can shield the mutual attraction between the nanoparticles to a certain extent.

[0019] Preferably, multiple sets of liquid supply components are arranged on the liquid storage cylinder 303, and the multiple sets of liquid supply components are arranged in a state of circular array. The liquid supply component includes a liquid outlet pipe 601 fixed to the liquid storage cylinder 303. Both ends of the liquid outlet pipe 601 communicate with the inside of the liquid storage cylinder 303 and the mixing cylinder 301 respectively. One end of the liquid outlet pipe 601 located inside the mixing cylinder 301 is connected with an L-shaped pipe 602 through a bellows pipe 603. A telescopic component for telescopically connecting the L-shaped pipe 602 is arranged on one side of the liquid storage cylinder 303, and a pushing component for pushing the L-shaped pipe 602 is arranged inside the mixing cylinder 301; It should be noted here that: through the extrusion of the piston plate 1001, the buffer liquid inside the piston cylinder 304 is transported to the inside of the mixing cylinder 301 through the liquid storage cylinder 303, the liquid outlet pipe 601, the bellows pipe 603 and the L-shaped pipe 602. Through the transportation of the nano-carrier and the buffer liquid, the buffer liquid and the nano-carrier are mixed inside the mixing cylinder 301.

[0020] Preferably, the telescopic component includes a mounting ring 701 sleeved outside the L-shaped pipe 602. An L-shaped frame 702 is fixed on the mounting ring 701. Multiple sets of sleeves 703 are fixed on the L-shaped frame 702. A sliding rod 704 is slidably connected to the sleeve 703. One end of the sliding rod 704 is fixed with a connecting plate 705, and the connecting plate 705 is fixed to the front end of the liquid storage cylinder 303. A first spring 706 is sleeved outside the sleeve 703, and both ends of the first spring 706 are connected with the L-shaped frame 702 and the connecting plate 705 respectively; It should be noted here that: through multiple sets of sleeves 703 and sliding rods 704, the telescopic guidance of the transmission pin 801 and the L-shaped frame 702 after being stressed is assisted. Through each first spring 706, it is convenient for the transmission pin 801 and the L-shaped frame 702 to return after the contraction movement.

[0021] Preferably, the pushing component includes a transmission pin 801 fixed to the L-shaped frame 702. Multiple sets of spherical protrusions 802 for transmitting force by abutting against the end of the transmission pin 801 are fixed in a state of circular array inside the mixing cylinder 301; It should be noted here that: during the rotation of the liquid storage cylinder 303 and the L-shaped pipe 602, the end of the transmission pin 801 on the L-shaped frame 702 abuts against the spherical protrusions 802 inside the mixing cylinder 301 in turn. During the abutting process, the transmission pin 801, the L-shaped frame 702, the mounting ring 701 and the L-shaped pipe 602 are forced to perform a centering contraction movement. When the end of the transmission pin 801 does not abut against the spherical protrusion 802, through the elastic force of the first spring 706 on the telescopic component, the transmission pin 801, the L-shaped frame 702, the mounting ring 701 and the L-shaped pipe 602 are forced to return, so that the L-shaped pipe 602 performs a reciprocating gathering and dispersing movement while rotating.

[0022] Preferably, the rotating assembly includes a mounting hole 401 formed at the end of the mixing cylinder 301. An annular groove 402 is formed inside the mounting hole 401. An annular plate 403 is rotatably connected inside the annular groove 402. The annular plate 403 is sleeved and fixed on the outer side of the liquid storage cylinder 303. The cross-sections of the annular groove 402 and the annular plate 403 are arranged in a T shape. It should be noted here that: through the mounting hole 401, the annular groove 402 and the annular plate 403, the rotatable connection of the liquid storage cylinder 303 is facilitated.

[0023] Preferably, the driving assembly includes a gear ring 501 sleeved and fixed on the outer side of the liquid storage cylinder 303. A fixing frame 502 is fixed on the outer side of the mixing cylinder 301. A rotating shaft 503 is rotatably connected to the fixing frame 502. A gear 504 is fixed on the rotating shaft 503. The gear 504 is meshed with the gear ring 501. A driving motor 505 for driving the rotating shaft 503 is installed on the fixing frame 502. It should be noted here that: through the driving motor 505, the gear 504 on the rotating shaft 503 is driven to rotate. During the rotation of the gear 504, through the meshing transmission between the gear 504 and the gear ring 501, the liquid storage cylinder 303 is driven to move under force.

[0024] Preferably, the connecting assembly includes a connecting frame 901 fixed on the operating frame 1. A fixing plate 902 for connecting and fixing the piston cylinder 304 is fixed between the connecting frame 901 and the outer side of the piston cylinder 304. It should be noted here that: through the connecting frame 901 and the fixing plate 902, the fixed connection of the piston cylinder 304 is facilitated.

[0025] Preferably, the extrusion assembly includes a piston plate 1001 slidably connected inside the piston cylinder 304 and the L-shaped piston tube 2. An extrusion rod 1002 is fixed on the piston plate 1001. A transmission assembly for transmitting the extrusion rod 1002 is arranged on the connecting frame 901. It should be noted here that: through the transmission assembly, the two piston plates 1001 slide inside the L-shaped piston tube 2 and the piston cylinder 304 respectively. During the sliding process of the piston plate 1001 inside the L-shaped piston tube 2, through the extrusion action of the piston plate 1001, the nano-carriers filled inside the L-shaped piston tube 2 are extruded and conveyed into the mixing cylinder 301 and finally injected into the organism through the syringe needle 302. During the sliding process of the piston plate 1001 inside the piston cylinder 304, through the extrusion action of the piston plate 1001, the buffer solution inside the piston cylinder 304 is conveyed into the mixing cylinder 301 through the liquid storage cylinder 303, the liquid outlet pipe 601, the bellows pipe 603 and the L-shaped pipe 602.

[0026] Preferably, the transmission assembly includes a transmission plate 1101. One ends of two sets of extrusion rods 1002 are fixed to the transmission plate 1101. A rectangular plate 1102 is fixed to one side of the transmission plate 1101. A support frame 1103 is fixed to the connecting frame 901. A cylinder 1104 for driving the rectangular plate 1102 is installed on the support frame 1103. It should be noted here that: due to the driving effect of the cylinder 1104, it is convenient for the rectangular plate 1102 and the transmission plate 1101 to be stressed and move. During the movement of the transmission plate 1101, the piston plate 1001 is driven to move through the connection of the extrusion rod 1002.

[0027] In this solution: an enzymatic hydrolysis device for regulating histone acetylation by targeting SIRT1 includes the following steps: During the process of using the enzymatic hydrolysis device to perform enzymatic hydrolysis on cells, it is necessary to introduce the nanocarrier loaded with the enzymatic hydrolysis regulation component into the organism by intravenous injection. During the injection process, the nanocarrier is pre-filled inside the L-shaped piston tube 2, and then the pre-prepared buffer solution is filled inside the piston cylinder 304. After filling, through the interaction of the transmission assembly and the extrusion assembly, the two piston plates 1001 slide inside the L-shaped piston tube 2 and the piston cylinder 304 respectively. During the process of the piston plate 1001 sliding inside the L-shaped piston tube 2, through the extrusion of the piston plate 1001, the nanocarrier filled inside the L-shaped piston tube 2 is extruded and transported into the mixing cylinder 301 and finally injected into the organism through the syringe 302. During the process of the piston plate 1001 sliding inside the piston cylinder 304, through the extrusion of the piston plate 1001, the buffer solution inside the piston cylinder 304 is transported into the mixing cylinder 301 through the liquid storage cylinder 303, the liquid outlet pipe 601, the bellows pipe 603 and the L-shaped pipe 602. Through the transportation of the nanocarrier and the buffer solution, the buffer solution and the nanocarrier are mixed inside the mixing cylinder 301. During the mixing process, the buffer solution can adjust the PH value and ionic strength of the magnetic nanocarrier. The appropriate PH value can maintain the stability of the surface charge of the nanocarrier, causing electrostatic repulsion between the nanocarriers. At the same time, the buffer solution can adjust the ionic strength to avoid the compression of the double electric layer caused by high ionic strength, so as to ensure the effective exertion of the electrostatic repulsion formed by the surface charge of the nanocarrier and prevent the aggregation of the magnetic nanocarrier during injection and transportation. And during the process of delivering the buffer solution, through the mutual cooperation of the rotating component and the driving component, the liquid storage cylinder 303 is driven to rotate at the end of the mixing cylinder 301. During the rotation process, through the connection of the telescopic component and the liquid outlet pipe 601, each group of L-shaped pipes 602 is driven to rotate. Through the rotation of the L-shaped pipes 602 and the delivery of the buffer solution on the L-shaped pipes 602, while ensuring the mixing of the buffer solution and the nano-carriers, the mixed liquid is slightly stirred, increasing the dispersion degree of the nano-carriers in the fluid, further reducing the probability of agglomeration of the nano-carriers due to magnetic force. And during the rotation of the liquid storage cylinder 303 and the L-shaped pipes 602, the end of the transmission pin 801 on the L-shaped frame 702 abuts against the spherical protrusion 802 inside the mixing cylinder 301 in sequence. During the abutting process, the transmission pin 801, the L-shaped frame 702, the mounting ring 701 and the L-shaped pipes 602 are forced to perform a centering contraction movement (the contracted state is shown in Figure 9 ). And when the end of the transmission pin 801 does not abut against the spherical protrusion 802, through the elastic force of the first spring 706 on the telescopic component, the transmission pin 801, the L-shaped frame 702, the mounting ring 701 and the L-shaped pipes 602 are forced to reset (the reset state is shown in Figure 8 ), so that the L-shaped pipes 602 perform a reciprocating gathering and dispersing movement while rotating. Through the reciprocation and dispersion of the L-shaped pipes 602, it is convenient for the buffer solution delivered on the L-shaped pipes 602 to mix with the nano-carriers at different positions inside the mixing cylinder 301, ensuring the mixing effect of the nano-carriers and the buffer solution.

[0028] It should be noted that in this article, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the term "comprising", "including" or any other variant thereof is intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements not only includes those elements, but also includes other elements not expressly listed, or also includes elements inherent to this process, method, article or device.

[0029] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An enzymatic device for regulating histone acetylation by targeting SIRT (1), comprising: An operating frame (1) for delivering a nanocarrier, wherein the surface of the nanocarrier is modified with a ligand that specifically recognizes and binds to a SIRT (1) cell surface receptor, and the interior of the nanocarrier is provided with a magnetic material for assisting in targeting; It is characterized by further comprising: An L-shaped piston tube (2) disposed on the operating frame (1) and used for liquid delivery of nanocarriers, wherein the operating frame (1) is provided with a processing component for preventing agglomeration of the nanocarriers delivered inside the L-shaped piston tube (2); The processing assembly comprises a mixing cylinder (301) fixed on an operating frame (1); one end of an L-shaped piston tube (2) is in communication with the interior of the mixing cylinder (301); the front end of the mixing cylinder (301) is in communication with a needle tube (302); the rear end of the mixing cylinder (301) is rotatably connected to a liquid storage cylinder (303) via a rotating assembly; the liquid storage cylinder (303) is provided with a liquid supply assembly for supplying liquid to the interior of the mixing cylinder (301); the mixing cylinder (301) is provided with a driving assembly for driving the liquid storage cylinder (303); the ends of the liquid storage cylinder (303) are in communication with each other and are rotatably connected to a piston cylinder (304); the operating frame (1) is provided with a connecting assembly for connecting the piston cylinder (304); a buffer solution for maintaining the pH value and ionic strength of the nanocarrier is stored in the interior of the piston cylinder (304); and the L-shaped piston tube (2) and the piston cylinder (304) are provided with an extrusion assembly for assisting liquid discharge.

2. The enzymatic hydrolysis device for regulating histone acetylation by targeting SIRT1 according to claim 1, characterized in that: The liquid supply components are arranged in multiple groups on the liquid storage cylinder (303), and the multiple groups of liquid supply components are arranged in a state of a ring array. The liquid supply components include a liquid outlet pipe (601) fixed on the liquid storage cylinder (303), and the two ends of the liquid outlet pipe (601) are respectively connected to the inside of the liquid storage cylinder (303) and the mixing cylinder (301), and one end of the liquid outlet pipe (601) located inside the mixing cylinder (301) is connected to an L-shaped tube (602) through an organ pipe (603), and a telescopic component for telescopically connecting the L-shaped tube (602) is arranged on one side of the liquid storage cylinder (303), and a pushing component for pushing the L-shaped tube (602) is arranged inside the mixing cylinder (301).

3. The enzymatic hydrolysis device for regulating histone acetylation by targeting SIRT1 according to claim 2, characterized in that: The telescopic assembly comprises a mounting ring (701) sleeved on the outside of an L-shaped tube (602); an L-shaped frame (702) is fixed on the mounting ring (701); a plurality of sleeves (703) are fixed on the L-shaped frame (702); a sliding rod (704) is slidably connected to the sleeve (703); a connecting plate (705) is fixed to one end of the sliding rod (704); the connecting plate (705) is fixed to the front end of the liquid storage cylinder (303); a first spring (706) is sleeved on the outside of the sleeve (703); and two ends of the first spring (706) are respectively connected to the L-shaped frame (702) and the connecting plate (705).

4. The enzymatic hydrolysis device for regulating histone acetylation by targeting SIRT1 according to claim 3, characterized in that: The pushing assembly comprises a driving pin (801) fixed on an L-shaped frame (702), and a plurality of groups of spherical protrusions (802) for abutting against the ends of the driving pin (801) for transmission are fixed inside the mixing cylinder (301) in a circular array.

5. The enzymatic hydrolysis device for regulating histone acetylation by targeting SIRT1 according to claim 1, characterized in that: The rotating assembly comprises a mounting hole (401) formed at the end of the mixing cylinder (301); an annular groove (402) is formed on the inner side of the mounting hole (401); an annular plate (403) is rotatably connected to the interior of the annular groove (402); the annular plate (403) is sleeved and fixed on the outer side of the liquid storage cylinder (303); and the cross-sections of the annular groove (402) and the annular plate (403) are T-shaped.

6. The enzymatic hydrolysis device for regulating histone acetylation by targeting SIRT1 according to claim 5, characterized in that: The driving assembly comprises a gear ring (501) sleeved and fixed on the outside of the liquid storage cylinder (303); a fixing frame (502) is fixed on the outside of the mixing cylinder (301); a rotating shaft (503) is rotatably connected to the fixing frame (502); a gear (504) is fixed to the rotating shaft (503); the gear (504) and the gear ring (501) are meshed with each other; and a driving motor (505) for driving the rotating shaft (503) is installed on the fixing frame (502).

7. The enzymatic hydrolysis device for regulating histone acetylation by targeting SIRT1 according to claim 1, characterized in that: The connection assembly comprises a connection frame (901) fixed on the operating frame (1), and a fixing plate (902) for connecting and fixing the piston cylinder (304) is fixed between the connection frame (901) and the outer side of the piston cylinder (304).

8. The enzymatic hydrolysis device for regulating histone acetylation by targeting SIRT1 according to claim 7, characterized in that: The extrusion assembly comprises a piston plate (1001) slidably connected to the piston cylinder (304) and the interior of the L-shaped piston tube (2); an extrusion rod (1002) is fixed to the piston plate (1001); and a transmission assembly for transmitting the extrusion rod (1002) is provided on the connecting frame (901).

9. The enzymatic hydrolysis device for regulating histone acetylation by targeting SIRT1 according to claim 8, characterized in that: The transmission assembly comprises a transmission plate (1101), one end of the two groups of extrusion rods (1002) are fixed to the transmission plate (1101), a rectangular plate (1102) is fixed to one side of the transmission plate (1101), a support frame (1103) is fixed to the connecting frame (901), and a cylinder (1104) for transmitting the rectangular plate (1102) is installed on the support frame (1103).

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