Compound microbial agent for bottom mud pollution control and application method thereof

Abstract

The invention belongs to the technical field of bottom mud pollution treatment, and particularly relates to a compound microbial agent for bottom mud pollution treatment and an application method, the compound microbial agent comprises the following abundance raw materials: a fungus group, the fungus group is composed of 16.6-25.6% of monascus purpureus and 1-4.4% of saccharomyces cerevisiae; the bacterial population is composed of 11.5% to 21.5% of lactobacillus bulgaricus, 6.8% to 15.8% of Escherichia herbacei, 3.8% to 8.8% of clostridium reuteri, 2.8% to 6.8% of gorabacterium rennnii, 0.5% to 2.6% of lactobacillus acidophilus, 0.5% to 2.6% of marine Soxhlet bacillus and 0.5% to 3% of bacillus population. Through the synergistic effect of various microorganisms, organic pollutants in bottom mud can be efficiently degraded, nitrogen and phosphorus can be removed, the treatment efficiency can be remarkably improved, meanwhile, stink of a water area of a test section can be effectively removed, the water quality is improved, corresponding sewage indexes are reduced, secondary pollution is avoided by adopting a microbial remediation technology, and the method is suitable for large-scale popularization and application. Compared with an existing treatment method, the method is more environmentally friendly.

Description

Technical Field

[0001] The present invention relates to the technical field of sediment pollution control, and specifically provides a composite microbial agent for sediment pollution control and an application method thereof. Background Art

[0002] Sediment pollution refers to the pollution of the sediments in rivers and lakes. Sediment is the sediment of rivers and lakes and an important part of natural waters. When the water area is polluted, some pollutants in the water can be stored in the sediment through precipitation or particulate adsorption and be released again under appropriate conditions, becoming a secondary pollution source. This kind of pollution is called sediment pollution.

[0003] In the process of river and lake pollution control, the treatment of sediment pollution is one of the main difficulties and a relatively common environmental problem. There is a dynamic balance of absorption and release between the water body and the sediment. When the water body is severely polluted, some pollutants can enter the sediment through precipitation, adsorption and other effects; when the pollution caused by external sources is controlled, various organic and inorganic pollutants accumulated in the sediment re-enter the overlying water body through physical, chemical and biological exchange effects with the overlying water body, becoming a secondary pollution source affecting the water quality of the water body.

[0004] Due to the long-term accumulation of pollutants, sediment often contains a large amount of harmful substances such as organic pollutants, heavy metals, nitrogen and phosphorus. Traditional sediment treatment methods, such as physical methods (such as dredging, adsorption, etc.) and chemical methods (such as redox method, chemical flocculation, etc.), have certain disadvantages, such as high cost, serious secondary pollution and other problems. Therefore, the use of microbial remediation technology has become an important direction for sediment pollution control.

[0005] Microbial remediation technology degrades harmful substances in sediment by using the metabolic activities of microorganisms, and has the advantages of low cost, no secondary pollution, simple operation, etc.

[0006] It should be noted that the above content belongs to the technical cognition scope of the inventor and does not necessarily constitute the prior art. Summary of the Invention

[0007] Aiming at the deficiencies of the prior art, the present invention provides a composite microbial agent for sediment pollution control and an application method thereof, solving the existing problems.

[0008] To achieve the above object, the present invention provides the following technical solution: A composite microbial agent for sediment pollution control, comprising the following raw materials in abundance:

[0009] Fungal group, the fungal group is composed of Monascus purpureus 16.6 - 25.6% and Saccharomycopsis fibuligera 1 - 4.4%;

[0010] A bacterial community, which is composed of 11.5 - 21.5% of Lactobacillus jaguaris, 6.8 - 15.8% of Escherichia hermannii, 3.8 - 8.8% of Clostridium ljungdahlii, 2.8 - 6.8% of Lachnospiraceae bacterium CAG:253, 0.5 - 2.6% of Lactobacillus acidophilus, 0.5 - 2.6% of Marinobacter suessi, and 0.5 - 3% of Bacillus group.

[0011] In some of these embodiments, the Bacillus group is specifically composed of Bacillus licheniformis, Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus ginsengiterrae, Bacillus cereus, Bacillus coagulans, and Bacillus megaterium in a ratio of 1:1:1:1:1:1:1.

[0012] Another technical problem to be solved by the present invention is to provide an application method of a composite microbial agent for treating sediment pollution, including the following steps

[0013] Step 1: Select each fungal community and bacterial community, and screen out the best abundance combination through experiments;

[0014] Step 2: Conduct batch cultivation in a suitable culture medium to ensure the production, reproduction, and activity of the strains;

[0015] Step 3: Make the cultured strains into a composite microbial agent by one of freeze-drying, spray-drying, and liquid preservation to ensure its stable activity;

[0016] Step 4: Conduct activity detection and stability detection on the composite microbial agent to ensure its effectiveness and adaptability under different environmental conditions;

[0017] Step 5: Pretreat the test section of the water area;

[0018] Step 6: Take an activation water bucket, put the agent and brown sugar into it according to the ratio, crush them, and stir evenly after adding water;

[0019] Step 7: Add an aerator for aeration, and then turn off the machine and let it stand still;

[0020] Step 8: Stir evenly and sprinkle it onto the test section of the water area.

[0021] Further, the daily treatment capacity of the test section is calculated as 50M 3 Specifically: the length, width, and depth are: 15M * 3M (6M / 2) * 1M = 45M 3 , and the total amount of a small amount of new water flowing in is 50M 3 .

[0022] Further, before the treatment, it is necessary to conduct preliminary physical cleaning on the sediment to remove the floating objects and large particle impurities on the water surface of the test section of the water area.

[0023] Further, the aeration time of the aerator needs to be controlled within 1.5 hours, and then it is shut down and left standing for 3 hours.

[0024] Further, it is sprinkled once a day for the first 10 days. After the first application of the microbial agent, 5 kg of coral sand needs to be additionally scattered. The ratio of the microbial agent is 5 - 6 g / M 3 / day, the dosage of the microbial agent is 250 - 300 g / day, the dilution ratio is 1:50 by total mass, and the ratio of brown sugar is 2 - 5% by total mass. In case of rainy weather, the microbial agent is additionally applied once every time it rains.

[0025] Compared with the prior art, the present invention provides a composite microbial agent for treating sediment pollution and an application method, having the following beneficial effects:

[0026] The composite microbial agent for treating sediment pollution and the application method thereof, which is a composite microbial agent composed of a dominant flora, a fungal flora and a bacterial flora, through the synergistic action of various microorganisms, can efficiently degrade organic pollutants and remove nitrogen and phosphorus in the sediment, significantly improve the treatment efficiency, and at the same time can effectively remove the odor in the test section water area, thereby improving the water quality and reducing the corresponding sewage indexes. The use of the microbial remediation technology will not cause secondary pollution. Compared with the existing treatment methods, it is more environmentally friendly. The composite microbial agent of this method can adapt to the pollution of different types of sediment, has strong environmental adaptability, can be used in a variety of different environmental waters, and the composite microbial agent can play a long-term role in the sediment, reducing the dependence on artificial chemical methods and having a long persistence of the treatment effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 It is a schematic flow chart of the application method of a composite microbial agent for treating sediment pollution according to the present invention. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0028] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0029] It should be understood that the step numbers used in the text are only for convenient description and do not limit the execution order of the steps.

[0030] It should be understood that the terms used in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. As used in the specification of the present invention and the appended claims, unless the context clearly indicates otherwise, the singular forms "a", "an" and "the" are intended to include the plural forms.

[0031] The terms "comprising" and "including" indicate the presence of the described features, wholes, steps, operations, elements and / or components, but do not exclude the presence or addition of one or more other features, wholes, steps, operations, elements, components and / or their combinations.

[0032] The term "and / or" refers to any combination and all possible combinations of one or more of the associated listed items, and includes these combinations.

[0033] Please refer to Figure 1 , in this embodiment:

[0034] A composite microbial inoculant method for treating sediment pollution includes the following abundance raw materials:

[0035] Fungal group, the fungal group is composed of 21.6% Monascus purpureus and 2.4% Saccharomycopsis fibuligera;

[0036] Bacterial group, the bacterial group is composed of 16.5% Lactobacillus jaguaris, 8.8% Escherichia hermannii, 8.8% Clostridium lituseburense, 6.8% Lachnospiraceae bacterium L2-50, 2.6% Lactobacillus acidophilus, 2.6% Marinobacter suessi and 1% Bacillus group;

[0037] The Bacillus group is specifically composed of Bacillus licheniformis, Bacillus subtilis, Bacillus amyloliquefaciens, Geobacillus ginsengisoli, Bacillus cereus, Bacillus coagulans and Bacillus megaterium in a ratio of 1:1:1:1:1:1:1.

[0038] Based on the above composite microbial inoculant for treating sediment pollution, an application method of the composite microbial inoculant for treating sediment pollution is proposed, including the following steps:

[0039] Including the following steps:

[0040] Step 1: Select each fungal group and bacterial group, and screen out the best abundance combination through experiments;

[0041] Step 2: Conduct batch cultivation in a suitable culture medium to ensure the production, reproduction and activity of the strains;

[0042] Step 3: Make the cultured strains into a composite microbial inoculant by one of freeze-drying, spray-drying and liquid preservation to ensure the stability of its activity;

[0043] Step 4: Conduct activity detection and stability detection on the compound microbial inoculum to ensure its effectiveness and adaptability under different environmental conditions;

[0044] Step 5: Pretreat the water area of the test section;

[0045] The daily treatment volume of the test section is calculated as 50M 3 Specifically: the length, width, and depth are: 15M * 3M (6M / 2) * 0.5M = 45m, and the total amount of a small amount of new water inflow is 50M 3 ;

[0046] The water area position of the test section needs to be fixed firmly with a PVC enclosure, and the height of the enclosure should be more than 5CM lower than the plate position to prevent water flow from washing away the spilled bacteria;

[0047] Before treatment, it is necessary to conduct preliminary physical cleaning of the bottom mud to remove floating objects and large particle impurities on the water surface of the test section water area;

[0048] Step 6: Take the activation water bucket, put the inoculum and brown sugar into it according to the ratio, crush them, and stir evenly after adding water;

[0049] Step 7: Add an aerator for aeration, and then turn off the machine and let it stand still;

[0050] The aeration time of the aerator needs to be controlled for 1.5 hours, and turn off the machine and let it stand still for 3 hours;

[0051] Step 8: After stirring evenly, sprinkle it onto the water area of the test section;

[0052] Sprinkle it once a day for the first 10 days, and after the first sprinkling of the inoculum, 5 kg of coral sand needs to be additionally scattered. The inoculum ratio is 5 - 6g / M 3 / day, the inoculum dosage is 250 - 300g / day, the dilution ratio is 1:50 by total mass, and the brown sugar ratio is 2 - 5% by total mass. In case of rainy weather, add the inoculum once every time it rains;

[0053] The analysis data after the treatment of the test section water area in Example 1 is as follows in the table:

[0054]

[0055]

[0056] The data comparison of the bottom mud before and after use at the same sampling point is specifically as follows in the table:

[0057]

[0058] It can be seen from the above table that the total phosphorus, total nitrogen, and organic carbon content have decreased significantly.

[0059] Each embodiment in this specification is described in a progressive manner. For the same or similar parts among the embodiments, reference can be made to each other, and the key point of each embodiment is to illustrate the differences from other embodiments. In particular, for the system embodiment, since it is basically similar to the method embodiment, the description is relatively simple, and the relevant parts can be referred to the partial description of the method embodiment.

[0060] Finally, it should be noted that the above are only the preferred embodiments of the present invention and are not used to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions recorded in the foregoing embodiments or perform equivalent replacements for some of the technical features. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A composite microbial agent for treating sediment pollution, characterized in that: Including the following abundance materials: A fungal group, wherein the fungal group is composed of 16.6-25.6% of Monascus purpurogenum and 1-4.4% of Pseudomonas aeruginosa; The bacterial group is composed of 11.5-21.5% of Lactobacillus jaguariensis, 6.8-15.8% of Escherichia hermanni, 3.8-8.8% of Clostridium listeri, 2.8-6.8% of Loigobacillus rennieri, 0.5-2.6% of Lactobacillus acidophilus, 0.5-2.6% of Oceanobacterium sordellii and 0.5-3% of Bacillus group.

2. The composite microbial agent for treating sediment pollution according to claim 1, characterized in that: The Bacillus group is specifically composed of Bacillus licheniformis, Bacillus subtilis, Bacillus amyloliquefaciens, Geobacillus ginseng, Bacillus cereus, Bacillus coagulans and Bacillus megaterium in a ratio of 1:1:1:1:1:1:

1.

3. An application method of a composite microbial agent for sediment pollution control, characterized in that: The steps include: Step 1: Select each fungal group and bacterial group and screen out the best abundance combination through experiments; Step 2: Carry out batch culture in a suitable culture medium to ensure the production, reproduction and activity of the strain; Step 3: preparing the cultured strain into a composite microbial agent by freeze drying, spray drying and liquid storage to ensure its activity stability; Step 4: Conduct activity and stability tests on the composite microbial agent to ensure its effectiveness and adaptability under different environmental conditions; Step 5: Pre-treat the test section water area; Step 6: Take an activated water bucket, put the bacterial agent and brown sugar into it in proportion, crush them, add water and stir evenly; Step 7: Add an aerator for aeration, then shut down and let stand; Step 8: Stir evenly and sprinkle into the test area.

4. The method for applying the composite microbial agent for treating sediment pollution according to claim 3, characterized in that: The daily processing capacity of the test section in step 5 is 50M 3 Calculation, specifically: length, width, depth: 15M*3(6M / 2)*1M=45M 3 , and a small amount of new water flows in, totaling 50M 3 .

5. The method for applying the composite microbial agent for treating sediment pollution according to claim 3, characterized in that: Before the treatment, the bottom mud needs to be cleaned physically to remove floating objects and large particles of impurities on the surface of the water area of ​​the test section.

6. The method for applying the composite microbial agent for treating sediment pollution according to claim 3, characterized in that: The aeration time of the aerator in step 7 needs to be controlled within 1.5 hours, and the aerator should be shut down and left to stand for 3 hours.

7. The method for applying the composite microbial agent for treating sediment pollution according to claim 3, characterized in that: In step 8, the bacteria agent is sprayed once a day from the 1st to the 10th day, and 5 kg of coral sand needs to be added after the first spraying of the bacteria agent. The ratio of the bacteria agent is 5-6 g / M 3 / day, the dosage of the bacterial agent is 250-300g / day, the dilution amount is 1:50 according to the total mass ratio, and the brown sugar ratio is 2-5% according to the total mass ratio. In case of rainy weather, the bacterial agent is added once every time it rains.

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