Monascus purpureus PL-HQ8 with high yield of complex enzymes and exopolysaccharide and application thereof

Abstract

The application relates to the technical field of microorganisms, and particularly discloses a monascus ruber PL-HQ8 with high-yield composite enzymes and exopolysaccharide and an application thereof. The monascus ruber PL-HQ8 provided by the application is preserved in the China General Microbiological Culture Collection Center (CGMCC), has a preservation number of CGMCC No. 41598, and is preserved on November 15, 2024. The application also provides fermentation liquor, fermentation supernatant, composite enzyme preparation, exopolysaccharide extract and bacterial suspension prepared from the monascus ruber PL-HQ8. The monascus ruber PL-HQ8 can secrete multiple enzymes and exopolysaccharides, and when used in animal feed, can enhance the digestion and absorption capacity of the intestinal tract of animals to nutrients, improve the immunity, anti-inflammatory and anti-virus capacity of animals, and the feed utilization rate, so that the breeding benefit of animals is increased.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of microorganisms, in particular to a monascus purpureus PL-HQ8 with high yield of complex enzymes and exopolysaccharide and application thereof. BACKGROUND

[0002] Monascus as a traditional Chinese medicinal material has a long history of application; in recent years, with the development of biotechnology, the research on monascus has gradually deepened, especially the various metabolites with physiological activity produced during the fermentation of monascus have attracted widespread attention, these metabolites not only have important application in the field of medicine, but also are widely used in food fermentation, wine making, pigment production and traditional Chinese medicine manufacturing industries, the diversity and functionality of monascus make it an important microbial resource indispensable in modern industry.

[0003] In order to improve the quality and yield of monascus fermentation products, researchers have adopted various methods to optimize the fermentation process, common means include screening excellent strains, adjusting fermentation conditions, adding auxiliary materials, etc.; in addition, some studies have modified monascus through gene editing technology to enhance its enzyme production capacity and the types and contents of metabolites. Although the above methods improve the yield of monascus fermentation products to some extent, the existing monascus strains may exhibit unstable state during fermentation due to environmental factors, resulting in large fluctuations in product quality; especially in terms of enzyme production performance and exopolysaccharide production capacity, the performance of existing strains is often unsatisfactory, which limits the widespread application and development potential of monascus in industrial production.

[0004] Therefore, it is of great significance to screen a monascus with excellent enzyme production performance and exopolysaccharide production capacity. SUMMARY

[0005] In order to overcome the problem of poor enzyme production performance and exopolysaccharide production capacity of existing monascus, the present application provides a monascus purpureus PL-HQ8 with high yield of complex enzymes and exopolysaccharide and application thereof.

[0006] In a first aspect, the present application provides a monascus purpureus PL-HQ8, which adopts the following technical solution:

[0007] A monascus purpureus, wherein the monascus purpureus is Monascus purpureus PL-HQ8. Monascus purpureus) PL-HQ8 is preserved in the China General Microbiological Culture Collection Center (CGMCC), with the preservation number of CGMCC No. 41598 and the preservation time of November 15, 2024.

[0008] The purple Aspergillus PL-HQ8 provided in the application is screened from Fujian Gutian red koji rice, and can secrete multiple enzymes such as acid protease, neutral protease, alpha-amylase, glucoamylase, pectinase, xylanase, beta-mannanase and cellulase, and extracellular polysaccharide. The purple Aspergillus PL-HQ8 provided in the application is applied to feed production as a probiotic strain, which can improve the immunity, anti-inflammatory and anti-viral capacity of animals, protect the health of animals, enhance the digestion and absorption capacity of the intestinal tract of animals to nutrients, improve the utilization rate of feed by animals, and greatly increase the breeding benefit.

[0009] The culture characteristics of the purple Aspergillus PL-HQ8 provided in the application are shown in Table 1. Figure 2 As shown in Table 2, the colony of the purple Aspergillus PL-HQ8 is round and pellicular, and the wrinkles are obvious and raised in the middle. The mycelium edge is white, the colony is white in the early stage, the surface has aerial hyphae, the colony is pink in the middle stage, and gradually turns into purple red in the later stage. The back is dark red. The morphological characteristics of the purple Aspergillus PL-HQ8 are shown in Table 3. Figure 3 As shown in Table 3, the mycelium of the purple Aspergillus is 2-7 um in diameter, and is branched. The cleistothecia are ellipsoidal, 20-50 um in diameter, and have many ascospores in the cleistothecia. The ascospores are oval or spherical, and the conidia are round and generated from the mycelium.

[0010] In a second aspect, the application provides a fermentation broth, which comprises the purple Aspergillus PL-HQ8.

[0011] Optionally, the preparation method of the fermentation broth comprises the following steps:

[0012] (1) The purple Aspergillus PL-HQ8 is inoculated on a malt agar slant medium, and cultured at 28-35℃ for 4-7 days until the spores mature. Then the mature spores are washed with an appropriate amount of normal saline, and are put into a flask containing glass beads, and are shaken for 2-4 hours to disperse the spores. The spore suspension is filtered with four layers of gauze, and is diluted with an appropriate amount of normal saline. The spore concentration is counted with a hemocytometer, and the final spore concentration is adjusted to 10 7 -10 8 spores / mL to prepare a spore suspension;

[0013] (2) The spore suspension is inoculated into a seed culture medium at a volume ratio of 5-10%, and is cultured at 28-32℃ and 150-200 r / min for 72-96 hours to prepare a seed culture solution.

[0014] (3) the seed culture solution is inoculated into the fermentation medium at a volume ratio of 5%-10%, 8-10L of the fermentation medium is loaded into a 20L fermentation tank, sterilized at 121℃ for 20min, the temperature is reduced to 28-32℃, and the pressure of the fermentation tank is 0.05MPa; the initial fermentation control conditions are as follows: the temperature is 28-30℃, the initial pH of the fermentation medium is 5.5-6.0, the gas flow is 0.4-0.6m 3 / h, the stirring speed is 250-300r / min, and the dissolved oxygen is maintained at 20-30%; the pH is adjusted by automatic ammonia water feeding during the fermentation process, and the pH is controlled at 5.5-6.0; when the fermentation is performed for 5d, the stirring speed is adjusted to 200-250r / min, and the fermentation is ended when the fermentation is performed for 6d, thereby obtaining the Monascus purpureus PL-HQ8 fermentation liquor.

[0015] In a third aspect, the present application provides a fermentation supernatant, which is the supernatant obtained after centrifugation of the fermentation liquor.

[0016] In a fourth aspect, the present application provides a complex enzyme preparation, which comprises the Monascus purpureus PL-HQ8, the fermentation liquor or the fermentation supernatant.

[0017] Optionally, the preparation method of the complex enzyme preparation comprises the following steps: the Monascus purpureus PL-HQ8 fermentation liquor is centrifuged at 8000-10000r / min for 20-30min, and the supernatant is taken; 70% ammonium sulfate powder is added to the supernatant under ice bath conditions, and the precipitation is performed at 4±2℃ for 10-12h, and the precipitate is taken; the precipitate is redissolved with Tris-HCl buffer, and dialysis is performed at 4±2℃, thereby obtaining the Monascus purpureus PL-HQ8 complex enzyme preparation.

[0018] Optionally, the complex enzyme preparation comprises one or more enzymes selected from the group consisting of acid protease, neutral protease, alpha-amylase and glucoamylase, pectinase, xylanase, beta-mannanase and cellulase.

[0019] In a fifth aspect, the present application provides an exopolysaccharide extract, which is prepared from the fermentation liquor or the fermentation supernatant.

[0020] Optionally, the preparation method of the exopolysaccharide extract comprises the following steps:

[0021] (1) the Monascus purpureus PL-HQ8 fermentation liquor is centrifuged at 8000-10000r / min for 10-15min, the precipitate is discarded, and the supernatant is collected, thereby obtaining the Monascus purpureus PL-HQ8 fermentation supernatant;

[0022] (2) Then slowly add 700-900 mg / mL trichloroacetic acid to the fermentation supernatant of Monascus purpureus PL-HQ8, so that the final concentration is 40±5 mg / mL, and stand at 4±2℃ for 6-10 hours, then centrifuge at 8000-10000 r / min for 10-15 minutes, discard the precipitate, and collect the supernatant; then add anhydrous ethanol to the supernatant at a volume ratio of 1: (3.5-4.5), mix well, and stand at 4±2℃ for 20-24 hours, then centrifuge at 8000-10000 r / min for 10-15 minutes, and collect the bottom precipitate; finally, dissolve the precipitate in distilled water, centrifuge at 8000-10000 r / min for 10-15 minutes, discard the bottom insoluble precipitate, and collect the supernatant, which is the extracellular polysaccharide extract of Monascus purpureus PL-HQ8.

[0023] In a sixth aspect, the present application provides a bacterial suspension, which comprises Monascus purpureus PL-HQ8.

[0024] In a seventh aspect, the present application provides the use of Monascus purpureus PL-HQ8, fermentation broth, fermentation supernatant, complex enzyme preparation, extracellular polysaccharide extract or bacterial suspension in animal feed or animal feed additive.

[0025] In summary, the present application has the following beneficial effects:

[0026] 1. The present application provides a Monascus purpureus (Monascus purpureus) PL-HQ8. Monascus purpureus) PL-HQ8, deposited in the China General Microbiological Culture Collection Center (CGMCC), with the accession number CGMCC No. 41598, on November 15, 2024.

[0027] 2. The Monascus purpureus PL-HQ8 provided by the present application can secrete acid protease, neutral protease, alpha-amylase, glucoamylase, pectinase, xylanase, beta-mannanase, cellulase and other enzymes. When the Monascus purpureus PL-HQ8 provided by the present application is used as a probiotic strain in feed, the enzymes produced by the Monascus purpureus PL-HQ8 can promote the rupture of the component cell wall in animal feed, increase the degradation rate of the cell wall, promote the sufficient digestion and absorption of nutrients by animals, and thus improve the growth performance of animals and increase the breeding benefit.

[0028] 3. The Monascus purpureus PL-HQ8 provided by the present application can secrete extracellular polysaccharide, and the yield of extracellular polysaccharide is high. When the above-mentioned Monascus purpureus PL-HQ8 is used as a probiotic strain in feed, the extracellular polysaccharide produced by the Monascus purpureus PL-HQ8 has good antioxidant properties, which can enhance the immunity, anti-inflammatory and anti-viral ability of animals, protect the health of animals, and thus reduce the breeding cost and improve the breeding benefit. BRIEF DESCRIPTION OF DRAWINGS

[0029] Figure 1 is the 18S rDNA phylogenetic tree of Monascus purpureus PL-HQ8;

[0030] Figure 2 is the culture characteristic map of Monascus purpureus PL-HQ8;

[0031] Figure 3 is the morphological characteristic map of Monascus purpureus PL-HQ8. DETAILED DESCRIPTION

[0032] The present application is further described in detail below with reference to the accompanying drawings and examples, and it is particularly pointed out that the following examples are not to be construed as limiting the present application, and that the materials used in the following examples are available from ordinary commercial sources unless otherwise specified.

[0033] The present application provides a Monascus purpureus PL-HQ8, which is screened from Fujian Gutian Monascus rice, and identified by 18S rDNA as Monascus purpureus (Monascus purpureus). Monascus purpureus) , named Monascus purpureus PL-HQ8, preserved in the China General Microbiological Culture Collection Center (CGMCC), with the preservation number of CGMCC No. 41598, and the preservation time of November 15, 2024.

[0034] The culture medium and raw material sources involved in the examples of the present application are as follows:

[0035] The components of the enrichment medium are malt extract powder 130.0 g / L and chloramphenicol 0.1 g / L;

[0036] The components of the malt agar medium are malt extract powder 130.0 g / L, chloramphenicol 0.1 g / L, and agar 15.0 g / L;

[0037] The components of the seed culture medium are malt extract powder 130.0 g / L, potassium dihydrogen phosphate 1.0 g / L, magnesium sulfate 0.5 g / L, and ferric sulfate 0.01 g / L;

[0038] The components of the fermentation medium are malt extract powder 50.0 g / L, peptone 20.0 g / L, yeast extract powder 10.0 g / L, potassium dihydrogen phosphate 2.0 g / L, magnesium sulfate 0.5 g / L, ferric sulfate 0.01 g / L, manganese sulfate 0.006 g / L, and zinc sulfate 0.006 g / L.

[0039] The present application is further described in detail below with reference to the accompanying drawings and examples, and it is particularly pointed out that the following examples are not to be construed as limiting the present application, and that the materials used in the following examples are available from ordinary commercial sources unless otherwise specified. Example 1

[0040] Isolation and identification of the strain

[0041] (1) Isolation and screening: Use Fujian Gutian red yeast rice as a sample to screen Monascus; take 1 g of sample in 100 mL of enrichment medium, fully shake at 180 r / min and 30°C for 24 h, take the dilution to spread on malt agar plates, incubate at 30°C inverted for 7-10 days to observe the growth of colonies; pick a single colony and perform plate streak purification for more than 3 times to obtain a purified strain;

[0042] The cultural characteristics of the purified strains were as follows: Figure 2 As shown in the figure, the colony is circular and membranous with obvious wrinkles and a bulge in the middle. The edge of the mycelium is white. The colony is white in the early stage, with aerial hyphae on the surface. It is light red in the middle stage, gradually turns purple-red in the late stage, and dark red on the back. Figure 3 As shown, the hyphae are 2-7um in diameter, branched, and septate. The cleistothecia are ellipsoidal, 20-50um in diameter. Numerous asci are scattered in the cleistothecia. Ascospores are ovoid or spherical. Conidia are born from the hyphae and are round.

[0043] (2) Identification: The purified strain was inoculated into enrichment medium and cultured at 30°C for 24 h for morphological identification and 18SrDNA molecular biological identification. The strain was sequenced and identified by Beijing Liuhe BGI Genomics Co., Ltd. The sequence results were compared with the sequences in Genbank by Blast analysis, and a phylogenetic tree was constructed using Mega 6.0 software, as shown in the following example: Figure 1 .

[0044] Figure 1 Results showed that the purified strain was homologous to the Monascus purpureus published by NCBI. Combined with traditional morphological characterization, the isolate was further identified as Monascus purpureus and named PL-HQ8. Monascus purpureus PL-HQ8 was deposited with the General Microbiology Center of the China Culture Collection Administration under the accession number CGMCC No. 41598 and the date of deposit: November 15, 2024. Example 2

[0045] Example 2 provides a Monascus purpurogenum PL-HQ8 fermentation broth, the preparation method of which comprises the following steps:

[0046] (1) A single colony of Monascus purpurogenus PL-HQ8 was inoculated into a malt agar slant medium and cultured at 30°C for 5 days until the spores matured. The mature spores were then washed with an appropriate amount of physiological saline and placed in a triangular flask containing glass beads. The spores were shaken for 2 hours to break up the spores. The spore suspension was filtered through four layers of gauze and diluted with an appropriate amount of physiological saline. The spore concentration was counted using a hemocytometer and the final spore concentration was adjusted to 10 8 / mL, and a spore suspension of Monascus PL-HQ8 was prepared;

[0047] (2) The spore suspension of Monascus purpureus PL-HQ8 was inoculated into the seed culture medium at a volume ratio of 8%, and cultured at 30°C and 180 r / min for 96 h to prepare a seed culture solution;

[0048] (3) 10 L of fermentation medium was loaded into a 20 L fermenter, sterilized at 121°C for 20 min, and the temperature was reduced to 28-30°C. The seed culture solution was inoculated into the fermenter at a volume ratio of 8%, and the pressure of the fermenter was 0.05 MPa. The initial fermentation control conditions were as follows: temperature 28-30°C, initial pH of the fermentation medium 5.5-6.0, gas flow rate 0.4-0.6 m 3 / h, stirring speed 280 r / min, and the dissolved oxygen was maintained at 25%. The pH was adjusted by automatic ammonia water feeding during the fermentation process to control the pH at 5.5-6.0. When the fermentation time was 5 d, the stirring speed was adjusted to 230 r / min, and the fermentation was completed when the fermentation time was 6 d to prepare the Monascus purpureus PL-HQ8 fermentation liquor. Example 3

[0049] Example 3 provides a Monascus purpureus PL-HQ8 complex enzyme preparation, and the preparation method comprises the following steps:

[0050] The Monascus purpureus PL-HQ8 fermentation liquor obtained in Example 2 was centrifuged at 10,000 r / min for 20 min, and the supernatant was taken. Under ice bath conditions, 70% ammonium sulfate powder was added to the supernatant, and the precipitate was taken after precipitation at 4°C for 12 h. The precipitate was redissolved with Tris-HCl buffer and dialyzed at 4°C to obtain the Monascus purpureus PL-HQ8 complex enzyme preparation. Comparative Example 1

[0051] Comparative Example 1 provides a Monascus purpureus Mp-42 fermentation liquor, and the preparation method comprises the following steps:

[0052] (1) Mp-42 strain was inoculated into a starch separation culture medium and cultured at 30°C for 7 d. Six 0.9 cm punches were punched out from the bacterial cake and inoculated into 50 mL of seed culture medium, and cultured at 180 r / min for 3 d. Then, mature spores were washed with an appropriate amount of physiological saline, loaded into a flask containing glass beads, and shaken for 2 h to disperse the spores. The spore suspension was filtered with four layers of gauze, diluted with an appropriate amount of physiological saline, and the spore concentration was counted with a hemocytometer. The final spore concentration was adjusted to 10 8 individuals / mL to prepare a Monascus purpureus Mp-42 spore suspension;

[0053] (2) The Monascus purpureus Mp-42 spore suspension was inoculated into the fermentation medium at a volume ratio of 8%, and cultured at 30°C and 180 r / min for 7 d to prepare a Monascus purpureus Mp-42 fermentation liquor. Comparative Example 2

[0054] Comparative Example 2 provides a purple Monascus Mp-42 complex enzyme preparation, and the preparation method comprises the following steps:

[0055] The purple Monascus PL-HQ8 fermentation broth obtained in Comparative Example 1 was centrifuged at 8000 r / min for 10 min, and the supernatant was taken. Under ice bath conditions, 70% ammonium sulfate powder was added to the supernatant, and the precipitate was taken after precipitation at 4°C for 12 h. The precipitate was redissolved with Tris-HCl buffer and dialyzed at 4°C to obtain a purple Monascus Mp-42 complex enzyme preparation.

[0056] Enzyme production performance detection

[0057] The enzyme activities of protease, alpha-amylase, saccharifying enzyme, cellulase, beta-mannanase, pectinase, and xylanase in the complex enzyme preparations obtained in Example 3 and Comparative Example 2 were detected, and the detection results are shown in Table 1.

[0058] The determination of protease activity was carried out according to GB / T 28715-2012 Proteinase Activity Determination Method; the determination of alpha-amylase activity was carried out according to DB 13T1095-2009 Alpha Spectrophotometric Method for Enzyme Preparation for Feedstuff; the determination of saccharifying enzyme activity was carried out according to QB / T1803-1993 General Test Method for Industrial Enzyme Preparation; the determination of cellulase activity was carried out according to NY / T 912-2004 Spectrophotometric Method for Determination of Cellulase Activity in Feedstuff Additives; the determination of beta-mannanase activity was carried out according to GB / T 36861-2018 Method for Determination of Beta-mannanase Activity in Feedstuff Additives; the determination of pectinase activity was carried out according to QB 1502-92 Method for Determination of Pectinase Activity in Pectinase Preparation; and the determination of xylanase activity was carried out according to GB / T 23874-2009 Spectrophotometric Method for Determination of Xylanase Activity in Feedstuff Additives.

[0059] Table 1 Determination results of enzyme activities in the complex enzyme preparations obtained in Example 3 and Comparative Example 2

[0060]

[0061] According to the detection results in Table 1, the purple Aspergillus PL-HQ8 provided in the application can produce acid protease, neutral protease, alpha-amylase and glucoamylase, pectinase, xylanase, beta-mannanase and cellulase at the same time through one fermentation; the glucoamylase activity of the complex enzyme preparation obtained through treatment is as high as 1513 U / mL, the pectinase activity is as high as 929 U / mL, and the acid protease activity is as high as 356.8 U / mL. Therefore, it is illustrated that the purple Aspergillus PL-HQ8 provided in the application can be applied to feed as a probiotic strain, promotes the degradation of feed, improves the absorption of nutritional ingredients of feed by animals, improves the growth performance of animals, and further increases the breeding benefit. Example 4

[0062] Example 4 provides an exopolysaccharide extract of the purple Aspergillus PL-HQ8, and the preparation method thereof comprises the following steps:

[0063] (1) The fermentation broth of the purple Aspergillus PL-HQ8 obtained in Example 2 is centrifuged at 10000 r / min for 10 min, and the precipitate is discarded, and the supernatant is collected to obtain the fermentation supernatant of the purple Aspergillus PL-HQ8;

[0064] (2) Then, 800 mg / mL trichloroacetic acid is slowly added to the fermentation supernatant of the purple Aspergillus PL-HQ8 to make the final concentration 40 mg / mL, and it is placed at 4°C for 8 h, and then centrifuged at 10000 r / min for 10 min, and the precipitate is discarded, and the supernatant is collected; then anhydrous ethanol is added to the supernatant at a volume ratio of 1:4, and after mixing, it is placed at 4°C for 24 h, and then centrifuged at 10000 r / min for 10 min, and the bottom precipitate is collected; finally, the precipitate is dissolved in distilled water, centrifuged at 10000 r / min for 10 min, the bottom insoluble precipitate is discarded, and the supernatant is collected, which is the exopolysaccharide extract of the purple Aspergillus PL-HQ8. Comparative Example 3

[0065] Comparative Example 3 provides an exopolysaccharide extract of the purple Aspergillus Mp-42.

[0066] The exopolysaccharide extract of the purple Aspergillus Mp-42 is made from the fermentation broth of the purple Aspergillus Mp-42 provided in Comparative Example 2, and the preparation method is the same as that of Example 4.

[0067] Performance detection

[0068] The exopolysaccharide production performance and antioxidant performance of the exopolysaccharide extracts obtained in Example 4 and Comparative Example 3 are detected, and the results are shown in Table 2.

[0069] (1) The exopolysaccharide content in the exopolysaccharide extract is detected by the phenol-concentrated sulfuric acid method;

[0070] (2) Antioxidant properties: The exopolysaccharide solution was centrifuged at 10,000 r / min for 10 min, the precipitate was retained, dried at 80°C, weighed, and then prepared into a 0.5 mg / mL polysaccharide solution with purified water for the following test;

[0071] Determination of DPPH radical scavenging activity: 2 mL of polysaccharide solution was added to 2 mL of DPPH solution (concentration of 1×10 - 4 mol / L), mix well, react at room temperature for 30 minutes, and measure the absorbance Ai at 517nm. At the same time, measure the absorbance A0 of 2mL DPPH solution + 2mL ethanol and the absorbance Aj of 2mL polysaccharide solution + 2mL ethanol. Each experiment was performed in parallel 3 times, and the average value was taken. The DPPH free radical scavenging rate was calculated as follows:

[0072] DPPH free radical scavenging rate (%) = (1-(Ai-Aj) / A0) × 100%

[0073] Determination of hydroxyl radical scavenging activity: Take 1 mL of 8.8 mmol / L salicylic acid, add 1 mL of PBS buffer and 1 mL of polysaccharide solution in sequence, mix thoroughly, then add 1 mL of 9 mmol / L FeSO4 and 1 mL of 9 mmol / L H2O2, react at 37°C for 60 min, take the supernatant, and then detect the absorbance value A at 510 nm. i ; 1mL distilled water was used to replace the polysaccharide solution to determine the absorbance value A0; distilled water was used to replace H2O2 to determine the absorbance value A j Each experiment was repeated three times and the average value was taken. The hydroxyl radical scavenging rate was calculated as follows:

[0074] Hydroxyl radical scavenging rate (%) = ((1-(Ai-Aj) / A0) × 100%

[0075] Scavenging superoxide anion free radicals: Take 4.5mL, 0.05mol / L Tris-HCl buffer (pH=8.2), preheat in a 25℃ water bath for 20min, add 4.5mL polysaccharide solution and 0.5mL 25mmol / L pyrogallol solution respectively; mix well, react in a 25℃ water bath for 5min, then add 1mL, 8mol / L HCl to terminate the reaction, let it stand for 10min, and measure the absorbance value A at 299nm. i ; 1mL distilled water was used to replace the polysaccharide solution to determine the absorbance value A0; 0.5mL 10mmol / L HCl was used to replace the pyrogallol solution to determine the absorbance value A j Each experiment was repeated three times and the average value was taken. The calculation formula for superoxide anion radical scavenging rate is:

[0076] Superoxide anion radical scavenging rate (%) = (1 - (Ai - Aj) / A0) x 100%

[0077] Table 2: Results of detecting the content of exopolysaccharide in the exopolysaccharide extract obtained from Example 4 and Comparative Example 3

[0078]

[0079] According to the results of Table 2, it can be known that the exopolysaccharide production ability of the Monascus purpureus PL-HQ8 provided in the present application is obviously superior to that of the existing Monascus purpureus Mp-42, and the scavenging ability (antioxidation ability) of the Monascus purpureus PL-HQ8 to DPPH free radical, hydroxyl radical and superoxide radical is also superior to that of the Monascus purpureus Mp-42. Therefore, the Monascus purpureus PL-HQ8 provided in the present application can produce exopolysaccharide and has good antioxidation effect, and when it is used in feed, the immunity of animals can be improved, the health of animals can be protected, and the breeding benefit can be improved.

[0080] Although the present application has been described in detail with general description and specific embodiments above, some modifications or improvements can be made on the basis of the present application, which is obvious to those skilled in the art. Therefore, these modifications or improvements made on the basis of not deviating from the spirit of the present application, all belong to the scope of the present application claimed.

Claims

1. A species of Monascus purpurogenum ( Monascus purpureus PL-HQ8, characterized in that Preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No. 41598, and the preservation time is November 15, 2024; The Monascus purpureus PL-HQ8 can simultaneously secrete acid protease, neutral protease, alpha-amylase, glucoamylase, pectinase, xylanase, beta-mannanase, cellulase and extracellular polysaccharide.

2. A fermentation broth, characterized in that, The fermentation liquor comprises the Monascus purpureus PL-HQ8 in claim 1.

3. A complex enzyme preparation, characterized by, The complex enzyme preparation comprises the Monascus purpureus PL-HQ8 in claim 1 or the fermentation liquor in claim 2.

4. The complex enzyme preparation according to claim 3, characterized by, The complex enzyme preparation comprises acid protease, neutral protease, alpha-amylase, glucoamylase, pectinase, xylanase, beta-mannanase and cellulase produced by the Monascus purpureus PL-HQ8.

5. A bacterial suspension, characterized in that, The bacterial suspension comprises the Monascus purpureus PL-HQ8 in claim 1.

6. Use of the Monascus purpureus PL-HQ8 in claim 1, the fermentation liquor in claim 2, the complex enzyme preparation in claim 3 or 4, or the bacterial suspension in claim 5 in the preparation of animal feed or animal feed additive.

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