A method for identifying the origin of the traditional Chinese medicine Evodia rutaecarpa based on GC-IMS characteristic odor substances

Abstract

The present invention provides a method for identifying the base of Chinese medicine Evodia rutaecarpa based on GC-IMS characteristic odor substances: the sample is subjected to headspace GC-IMS detection, and when the peak volume ratio of 2(E)-hexenoic acid, dimethyl disulfide, 3-methylthiopropanol and methyl 2-methoxybenzoate is 1:(1.4-2.0):(1.4-2.4):(1.7-2.6), it is Evodia rutaecarpa; when its ratio is 1:(0.4-0.9):(0.6-1.0):(0.6-1.2), it is sparse hair Evodia rutaecarpa or stone tiger; then, when the peak volume ratio of cyclopentanone and left-rotating carvone is 5.3-5.7, it is sparse hair Evodia rutaecarpa; when the ratio is 6.2-7.6, it is stone tiger. The method of the present invention is fast, non-destructive, does not require complex sample pre-treatment, is simple, environmentally friendly, has low detection cost, and has intuitive results. It is suitable for quickly, objectively and accurately judging the base of Chinese medicine Evodia rutaecarpa in batches.

Description

Technical Field

[0001] The present invention belongs to the technical field of quality detection of Chinese medicinal materials or decoction pieces, and particularly relates to a method for identifying the origin of the Chinese medicinal material Evodia rutaecarpa. Background Art

[0002] The disclosure of this background information is intended to enhance understanding of the general background of the invention and should not necessarily be regarded as an acknowledgment or any form of suggestion that this information constitutes the prior art already known to a person skilled in the art.

[0003] Fructus evodiae is a plant of the Rutaceae family. Euodia rutaecarpa (Juss.) Benth., Shihu Euodia rutaecarpa (Juss) Benth.var. Officinalis (Dode) Huang or Evodia rutaecarpa Euodia rutaecarpa (Juss.) Benth. var. bodinieri The dried, nearly mature fruit of the plant (Dode) Huang, first described in the Shennong Bencao Jing (Shennong Bencao Jing) as a medium-grade herbal medicine, is a commonly used traditional Chinese medicine in clinical practice. Evodia rutaecarpa, which primarily contains alkaloids, bitter compounds, flavonoids, and volatile oils, exhibits anti-inflammatory, analgesic, anti-gastric ulcer, and antiemetic properties. It is commonly used clinically to treat difficult and complex conditions such as cardiovascular and digestive diseases and oral ulcers, with remarkable efficacy. However, as a multi-origin Chinese medicine, Evodia rutaecarpa exhibits highly similar morphological characteristics but significant variations in intrinsic quality, hindering quality control and precise clinical use.

[0004] Methods for identifying the origin of the traditional Chinese medicine Evodia rutaecarpa include trait identification, microscopic identification, physical and chemical identification, and DNA molecular identification. Traditional trait identification primarily evaluates the size and color of the medicinal material, believing that small, green seeds are the best for medicinal use. The "Compilation of Standards for the Specifications and Grades of Chinese Medicinal Materials" classifies Evodia rutaecarpa as "medium-flowered" and "small-flowered" based on market distribution, distinguished primarily by fruit diameter. However, small, large, medium, and small seeds cannot be used to identify origins. Some researchers have used fingerprinting to identify different origins of Evodia rutaecarpa. However, this method suffers from complex sample preparation and testing conditions, is time-consuming, requires high costs for reference materials and organic solvents, and carries certain risks and contamination risks associated with the reagents. Compared to traditional identification methods, DNA molecules can provide richer information for species identification. Studies have used universal DNA barcodes such as ITS, ITS2, and psbA-trnH for molecular identification of Evodia rutaecarpa. However, these methods cannot fully represent the genetic background of Evodia rutaecarpa species, and therefore cannot accurately identify the origin of Evodia rutaecarpa. Therefore, the development of a rapid and effective method for identifying the origin of the traditional Chinese medicine Evodia rutaecarpa is urgent and necessary.

[0005] The odor of most traditional Chinese medicines (TCMs) is closely related to their origin and quality. The odor of TCMs with different origins, origins, and processing methods can reflect differences in their volatile components. Therefore, the odor of TCMs is an important criterion for quality evaluation. Gas chromatography-ion mobility mass spectrometry (GC-IMS) is a newly emerging method for detecting the odor of TCMs. GC-IMS detection requires no complex sample pretreatment, is simple and environmentally friendly, uses few consumables during identification, and produces intuitive results. Therefore, exploring a method for identifying the origin of the TCM Evodia rutaecarpa (Evodia rutaecarpa) based on GC-IMS odor detection, enabling rapid, effective, and accurate identification of the origin of Evodia rutaecarpa medicinal materials or decoction pieces, is of great significance for improving Evodia rutaecarpa quality control methods and promoting clinical precision medicine use. Summary of the Invention

[0006] In response to the problem that identifying the origin of the traditional Chinese medicine Evodia rutaecarpa is difficult and the process is complicated in the existing technology, the present invention provides a method for identifying the origin of the traditional Chinese medicine Evodia rutaecarpa based on GC-IMS characteristic odor substances. The method does not require complicated sample pretreatment, is simple and environmentally friendly, uses fewer consumables in the identification process, and the results are intuitive.

[0007] To achieve the above objectives, the present invention adopts the following technical solutions.

[0008] A method for identifying the origin of the traditional Chinese medicine Evodia rutaecarpa based on GC-IMS characteristic odor substances comprises the following steps:

[0009] (1) The powder of the medicinal material or decoction piece of Evodia rutaecarpa to be tested is sampled by headspace injection and detected by gas chromatography-ion mobility spectrometry to obtain a characteristic fingerprint spectrum;

[0010] (2) Obtain the peak volumes of 2(E)-hexenoic acid, dimethyl disulfide, 3-methylthiopropanol, and methyl 2-methoxybenzoate, and make the following judgments:

[0011] When the ratio of the peak volumes of 2(E)-hexenoic acid, dimethyl disulfide, 3-methylthiopropanol and methyl 2-methoxybenzoate was 1:(1.4-2.0):(1.4-2.4):(1.7-2.6), the origin was determined to be Evodia rutaecarpa;

[0012] When the ratio of the peak volumes of 2(E)-hexenoic acid, dimethyl disulfide, 3-methylthiopropanol and methyl 2-methoxybenzoate is 1:(0.4-0.9):(0.6-1.0):(0.6-1.2), the origin is determined to be Evodia rutaecarpa or Evodia sparsely haired;

[0013] (3) When the origin is Evodia rutaecarpa or Evodia rutaecarpa, further obtain the peak volumes of cyclopentanone and L-carvone and make the following judgment:

[0014] When the ratio of the peak volumes of cyclopentanone and L-carvone is 5.3-5.7, the origin is determined to be Evodia rutaecarpa; when the ratio is 6.2-7.6, the origin is determined to be Lycopodiella vulgaris.

[0015] Furthermore, the headspace operation steps include: weighing 0.5-1 g of the powder of the Evodia rutaecarpa sample to be tested, placing it in a headspace injection bottle, incubating it at 80° C. for 15 minutes, and then injecting the headspace sample.

[0016] Furthermore, the gas chromatography-ion mobility mass spectrometry conditions are as follows: chromatographic column: MXT-5, 30 m, ID: 0.53 mm, df: 1.0 μm; chromatographic column temperature: 60°C; carrier gas / drift gas: N2; IMS temperature: 45°C; in the automatic headspace sampling unit, injection volume: 200 μL; incubation time: 15 min; incubation temperature: 80°C; injection needle temperature: 85°C; incubation speed: 500 rpm;

[0017] The gas chromatography conditions were as follows: E1 drift gas volume flow rate was 75 mL / min; E2 gas phase carrier gas volume flow rate: 0-2 min, 2 mL / min; 2-10 min, 2-10 mL / min; 10-20 min, 10-100 mL / min; 20-25 min, 100-150 mL / min; 25-40 min, 150 mL / min.

[0018] The present invention has the following advantages:

[0019] Through GC-IMS spectral analysis of three different origins of the traditional Chinese medicine Evodia rutaecarpa, a total of 86 volatile odorants were detected in Evodia rutaecarpa. Combining characteristic fingerprints with VIP analysis and screening, three characteristic odorants and one non-characteristic odorant were identified as distinguishing Evodia rutaecarpa from two other origins (Evodia sparse-hair and Evodia rutaecarpa). One characteristic odorant and one non-characteristic odorant were also identified as distinguishing Evodia sparse-hair from Evodia rutaecarpa. The ratio of characteristic to non-characteristic odorants allows for the identification of Evodia rutaecarpa from different origins, with high accuracy. This approach provides new insights into the identification of Evodia rutaecarpa medicinal materials or decoction pieces based on characteristic odorants.

[0020] The method provided by the present invention for identifying the origin of the traditional Chinese medicine Evodia rutaecarpa based on GC-IMS characteristic odor substances adopts the ratio of characteristic odor substances to non-characteristic odor substances, which can realize the identification of three origins and make the measurement results more accurate. The method of the present invention is rapid, non-destructive, does not require complex sample pretreatment, is simple and environmentally friendly, has low detection cost, and produces intuitive results. It is suitable for rapid, objective and accurate batch determination of the origin of the traditional Chinese medicine Evodia rutaecarpa. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 The GC-IMS three-dimensional spectra of Evodia rutaecarpa of different origins;

[0022] Figure 2 This is the GC-IMS two-dimensional top view of Evodia rutaecarpa of different origins;

[0023] Figure 3 This is the GC-IMS difference spectrum of Evodia rutaecarpa with different origins;

[0024] Figure 4 The GC-IMS characteristic fingerprints of Evodia rutaecarpa from different sources;

[0025] Figure 5 This is the OPLS-DA permutation test analysis diagram of Evodia rutaecarpa and two other original Evodia rutaecarpa species;

[0026] Figure 6 This is the VIP analysis diagram of Evodia rutaecarpa and two other basal Evodia rutaecarpa species;

[0027] Figure 7 This is the OPLS-DA permutation test analysis diagram of Evodia rutaecarpa and Evodia rutaecarpa;

[0028] Figure 8 This is the VIP analysis chart of Evodia rutaecarpa and Evodia sparsely haired;

[0029] Figure 9 This is the GC-IMS three-dimensional spectrum of the verification samples of Evodia rutaecarpa from different origins;

[0030] Figure 10 This is a 2D top view of the GC-IMS of the verification samples of Evodia rutaecarpa from different origins;

[0031] Figure 11 The GC-IMS difference spectra of the verification samples of Evodia rutaecarpa from different sources;

[0032] Figure 12 These are the GC-IMS characteristic fingerprints of the verified samples of the Chinese medicinal herb Evodia rutaecarpa from different origins. DETAILED DESCRIPTION

[0033] The present invention will be further described below with reference to the embodiments and drawings, but the present invention is not limited to the following embodiments.

[0034] Example 1 Establishment and verification of the method

[0035] (1) Method establishment

[0036] The Chinese medicinal materials Evodia rutaecarpa of different origins were collected, and the sources are shown in Table 1.

[0037] Table 1 Information of Evodia rutaecarpa samples

[0038]

[0039] 0.5 g of each of the powders of Evodia rutaecarpa (SM1, SM2, SM3, and SM4), Evodia rutaecarpa (SH1, SH2, SH3, and BZ4), and Evodia rutaecarpa (ZY1, ZY2, ZY3, and ZY4) listed in Table 1 were accurately weighed and placed in 20 mL headspace injection vials. The samples were incubated at 80°C for 15 min and then injected for determination by GC-IMS.

[0040] The GC-IMS conditions were as follows: MXT-5, 30 μm column, ID: 0.53 mm, df: 1.0 μm; column temperature, 60°C; carrier gas / drift gas: N2; IMS temperature, 45°C; injection volume in the automatic headspace sampling unit, 200 μL; incubation time: 15 min; incubation temperature: 80°C; injection needle temperature: 85°C; incubation speed: 500 rpm; gas chromatography conditions: E1 drift gas volume flow rate, 75 mL / min; E2 gas phase carrier gas volume flow rate: 0-2 min, 2 mL / min; 2-10 min, 2-10 mL / min; 10-20 min, 10-100 mL / min; 20-25 min, 100-150 mL / min; 25-40 min, 150 mL / min.

[0041] (2) Intuitive analysis

[0042] The Reporter plug-in built into the VOCal software was used to directly compare the differences in volatile odor compounds of the three original Evodia rutaecarpa. The GC-IMS three-dimensional spectra of Evodia rutaecarpa (SM1, SM2, SM3 and SM4), Evodia rutaecarpa (SH1, SH2, SH3 and SH4) and Evodia rutaecarpa (ZY1, ZY2, ZY3, ZY4) are shown in the figure. Figure 1 As shown, a peak in the three-dimensional spectrum represents a substance, red represents high concentration, and white represents low concentration.

[0043] The two-dimensional top view of Evodia rutaecarpa (SM1, SM2, SM3, SM4), Evodia rutaecarpa (SH1, SH2, SH3, SH4), and Evodia rutaecarpa (ZY1, ZY2, ZY3, ZY4) is shown in the figure. Figure 2 As shown, the horizontal axis is the ion migration time, the vertical line at the starting end is the reaction ion peak (RIP peak) after normalization, the vertical axis is the retention time of gas chromatography, each point on both sides of the RIP represents a volatile organic compound, and the color indicates the concentration of the organic compound. The darker the color, the greater the concentration.

[0044] The GC-IMS difference spectra of volatile odor substances of Evodia rutaecarpa (SM1, SM2, SM3, SM4), Evodia rutaecarpa (SH1, SH2, SH3, SH4), and Evodia rutaecarpa (ZY1, ZY2, ZY3, ZY4) are shown in the figure. Figure 3 As shown in the figure, the spectrum of Evodia rutaecarpa SM1 is used as a reference. If the volatile organic compounds of the two are consistent, the background after subtraction is white. Red indicates that the concentration of the substance is higher than the reference, and blue indicates that the concentration of the substance is lower than the reference. Figure 1-3 It can be seen that the volatile compounds of Evodia rutaecarpa and the other two basal Evodia rutaecarpa (Evodia sparse-hair and Evodia rutaecarpa) are quite different, while the overall difference between Evodia sparse-hair and Evodia rutaecarpa is relatively small.

[0045] (3) Fingerprint analysis

[0046] The Gallery Plot plug-in was used to compare the characteristic fingerprints formed and analyze the different odor substances. The GC-IMS characteristic fingerprints of Evodia rutaecarpa (SM1, SM2, SM3, SM4), Evodia rutaecarpa (SH1, SH2, SH3, SH4), and Evodia rutaecarpa (ZY1, ZY2, ZY3, ZY4) were obtained as follows Figure 4 As shown in the figure, each row represents all signal peaks selected from a single sample, and each column represents the signal peaks of the same volatile organic compound in different samples. Color represents the concentration of the compound, with darker red indicating a higher concentration. Qualitative analysis of volatile organic compounds was performed using the software's built-in NIST and IMS databases, resulting in the detection of 86 volatile organic compounds, primarily acids, aldehydes, ketones, alcohols, ethers, and esters. The peak volumes of dimethyl disulfide, 1-propanol, 3-(methylthio), and methyl 2-methoxybenzoate in Evodia rutaecarpa were relatively large, while the peak volumes of these components in the other two basal Evodia rutaecarpa species (Evodia sparse-hair and Evodia rutaecarpa var. ... The peak volumes of cyclopentanone in Evodia rutaecarpa and Evodia rutaecarpa samples are quite different, and can be used as a characteristic odor substance to identify the two, see Tables 2 and 3.

[0047] Table 2 Characteristic and non-characteristic odorants and peak volumes that distinguish Evodia rutaecarpa from two other Evodia rutaecarpa species

[0048]

[0049] Table 3 Characteristic and non-characteristic odorants and peak volumes for distinguishing Evodia rutaecarpa from Evodia rutaecarpa

[0050]

[0051] Taking the ratio range between the selected characteristic odor substances as the definition standard, no matter which of the three characteristic odor substances is used as the benchmark, the ratio ranges between the characteristic odor substances of the three origins of Evodia rutaecarpa partially overlap, and the origin cannot be identified, see Table 4.

[0052] Table 4 Ratio ranges based on the ratios between characteristic odor substances as the defining criteria

[0053]

[0054] Therefore, it is necessary to introduce other substances to complete the identification of the origin of Evodia rutaecarpa. Among the three originating Chinese medicinal herbs, the peak volume of 2(E)-hexenoic acid is relatively small compared to other volatile components, making it a suitable candidate for non-characteristic odorant. Based solely on the peak volume of cyclopentanone, a differential component found in samples of Evodia rutaecarpa and Ligusticum chuanxiong, the two originating species can be distinguished based on different peak volume ranges. However, considering that peak volume levels are affected by test time and sample batches, in order to improve the accuracy and reliability of the obtained results, non-characteristic odorants were also identified in the two species when distinguishing between Evodia rutaecarpa and Ligusticum chuanxiong. The ratio of characteristic to non-characteristic odorants was used to distinguish the origins. The peak volume of l-carvone in samples of Evodia rutaecarpa and Ligusticum chuanxiong was relatively small, and the differences in peak values ​​were minimal, making it a suitable candidate for non-characteristic odorant (see Tables 2 and 3).

[0055] To verify the rationality of the selected characteristic and non-characteristic odorants, Simca 14.1 software was used to perform orthogonal partial least squares discriminant analysis (OPLS-DA) using the GC-IMS peak volume information of the three basal Evodia species as variables, with Evodia samples as group "1" and Evodia sparse-hair and Evodia scabra samples as group "2". The model was validated using a permutation test, and variable importance projection analysis was performed. The larger the VIP value, the greater the contribution to distinguishing Evodia rutaecarpa from the other two basal Evodia species (Evodia sparse-hair and Evodia scabra). The model validation diagram and VIP diagram are shown in Figure 2. Figure 5 、 Figure 6. It can be seen that the established model is reliable. According to the VIP scores of odor substances, characteristic odor substances are searched for among odor substances with VIP values ​​≥ 1.3, and non-characteristic odor substances are searched for among odor substances with VIP ≤ 0.5. Through screening, dimethyl disulfide, 3-methylthiopropanol and methyl 2-methoxybenzoate are selected as characteristic odor substances for distinguishing Evodia rutaecarpa from the other two proto-Evodia rutaecarpa (Evodia sparse-hair and Evodia rutaecarpa), and 2(E)-hexenoic acid is selected as a non-characteristic odor substance for distinguishing Evodia rutaecarpa from the other two proto-Evodia rutaecarpa (Evodia sparse-hair and Evodia rutaecarpa). Further, the GC-IMS peak volume information of Evodia rutaecarpa and Evodia rutaecarpa was used as a variable, and the Evodia rutaecarpa samples were set as group "1" and the Evodia rutaecarpa samples as group "2" for VIP analysis. Model verification and VIP diagrams are shown in Fig. Figure 7 、 Figure 8 The established model is reliable. Similarly, based on the VIP scores of the odorants, characteristic odorants were searched for among odorants with VIP values ​​≥ 1.3, and non-characteristic odorants were searched for among odorants with VIP values ​​≤ 0.5. Through screening, cyclopentanone was determined to be the characteristic odorant for distinguishing Evodia rutaecarpa from Evodia rutaecarpa, and L-carvone was determined to be the non-characteristic odorant for distinguishing Evodia rutaecarpa from Evodia rutaecarpa. Through VIP value screening, it can be considered reasonable to use dimethyl disulfide, 3-methylthiopropanol, and methyl 2-methoxybenzoate as characteristic odorants for distinguishing Evodia rutaecarpa from the other two proto-Evodia rutaecarpa species (Evodia rutaecarpa and Evodia rutaecarpa), and to use 2(E)-hexenoic acid as a non-characteristic odorant. Furthermore, it is reasonable to use cyclopentanone as the characteristic odorant for distinguishing Evodia rutaecarpa from Evodia rutaecarpa, and L-carvone as a non-characteristic odorant.

[0056] The method for judging the origin of the Chinese herbal medicine Evodia rutaecarpa based on the peak volume ratio range of characteristic odor substances and non-characteristic odor substances in the Chinese herbal medicine Evodia rutaecarpa of different origins is: when the peak volume of the sample to be tested meets the ratio of 2(E)-hexenoic acid:dimethyl disulfide:3-methylthiopropanol:methyl 2-methoxybenzoate=1:(1.4-2.0):(1.4-2.4):(1.7-2.6), it is Evodia rutaecarpa; when the peak volume of the sample to be tested meets the ratio of 2(E)-hexenoic acid:dimethyl disulfide:3-methylthiopropanol:methyl 2-methoxybenzoate=1:(0.4-0.9):(0.6-1.0):(0.6-1.2), it is Evodia rutaecarpa or Evodia sparse-hair.

[0057] If the sample to be tested is determined to be Evodia rutaecarpa or Ligusticum scoparium, further origin identification is performed based on the volume ratio range of the cyclopentanone and L-carvone peaks. When the volume ratio range of the cyclopentanone and L-carvone peaks is 5.3-5.7, it is Evodia rutaecarpa, and when the ratio range is 6.2-7.6, it is Ligusticum scoparium.

[0058] Example 2 Practical application of the detection method

[0059] The samples of Evodia rutaecarpa (SM verification), Evodia rutaecarpa (SH verification) and Evodia rutaecarpa (ZY verification) in Table 1 were taken respectively and tested according to the test method and test conditions in Example 1 (1).

[0060] (1) Intuitive analysis

[0061] The GC-IMS three-dimensional spectra of Evodia rutaecarpa (SM verification), Evodia rutaecarpa (SH verification) and Evodia rutaecarpa (ZY verification) are shown in the figure. Figure 9 As shown, the two-dimensional top view of volatile odor substances is as follows Figure 10 As shown, the GC-IMS difference spectra of volatile odor substances are as follows Figure 11 As shown, the volatile odor compounds in the validation samples of Evodia rutaecarpa and the other two basal species (Evodia rutaecarpa sparse hair and Lepidoptera scoparia) differed significantly, while the overall differences between the validation samples of Evodia rutaecarpa sparse hair and Lepidoptera scoparia were relatively small. The validation samples (SM validation, SH validation, and ZY validation) showed little difference from the corresponding basal samples in Example 1.

[0062] (2) Fingerprint analysis

[0063] The characteristic fingerprints formed were compared using the Gallery Plot plug-in. The GC-IMS characteristic fingerprints of the three original Evodia rutaecarpa verification samples (SM verification, SH verification, and ZY verification) are as follows: Figure 12 As shown in Tables 5 and 6, the peak volumes of characteristic and non-characteristic odor substances in the three original Evodia rutaecarpa verification samples (SM verification, SH verification, and ZY verification) are shown.

[0064] Table 5 Peak volumes of characteristic and non-characteristic odorants in the validation samples that distinguish Evodia rutaecarpa from two other Evodia rutaecarpa species

[0065]

[0066] Table 6 Peak volumes of characteristic and non-characteristic odorants for distinguishing Evodia rutaecarpa and Evodia rutaecarpa in validation samples

[0067]

[0068] Calculations found that:

[0069] 1) The ZY verification sample had a ratio of 2(E)-hexenoic acid: dimethyl disulfide: 3-methylthiopropanol: methyl 2-methoxybenzoate of 1:1.6:1.7:1.7, indicating that it was Evodia rutaecarpa; the SM verification sample had a ratio of 2(E)-hexenoic acid: dimethyl disulfide: 3-methylthiopropanol: methyl 2-methoxybenzoate of 1:0.6:0.8:0.7; and the SH verification sample had a ratio of 2(E)-hexenoic acid: dimethyl disulfide: 3-methylthiopropanol: methyl 2-methoxybenzoate of 1:0.6:0.8:1.1, indicating that both were Evodia rutaecarpa or Evodia rutaecarpa;

[0070] 2) The SM validation sample had a cyclopentanone to L-carvone ratio of 5.6, indicating identification as Evodia rutaecarpa; the SH validation sample had a cyclopentanone to L-carvone ratio of 7.1, indicating identification as Evodia rutaecarpa. These sample validations demonstrate the accuracy and feasibility of the proposed method for identifying the origin of the traditional Chinese medicine Evodia rutaecarpa using the peak volume ratio range of characteristic and non-characteristic odorants using GC-IMS.

[0071] The foregoing is merely an embodiment of the present application and is not intended to limit the present application. For those skilled in the art, the present application may have various changes and variations. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present application should all be included within the scope of the claims of the present application.

Claims

1. A method for identifying the origin of the Chinese medicine Evodia rutaecarpa based on GC-IMS characteristic odor substances, characterized in that: The following steps are involved: (1) The powder of the medicinal material or decoction piece of Evodia rutaecarpa to be tested is sampled by headspace injection and detected by gas chromatography-ion mobility spectrometry to obtain a characteristic fingerprint spectrum; (2) Obtain the peak volumes of 2(E)-hexenoic acid, dimethyl disulfide, 3-methylthiopropanol, and methyl 2-methoxybenzoate, and make the following judgments: When the ratio of the peak volumes of 2(E)-hexenoic acid, dimethyl disulfide, 3-methylthiopropanol and methyl 2-methoxybenzoate was 1:(1.4-2.0):(1.4-2.4):(1.7-2.6), the origin was determined to be Evodia rutaecarpa; When the ratio of the peak volumes of 2(E)-hexenoic acid, dimethyl disulfide, 3-methylthiopropanol and methyl 2-methoxybenzoate is 1:(0.4-0.9):(0.6-1.0):(0.6-1.2), the origin is determined to be Evodia rutaecarpa or Evodia sparsely haired; (3) When the origin is Evodia rutaecarpa or Evodia rutaecarpa, further obtain the peak volumes of cyclopentanone and L-carvone and make the following judgment: When the ratio of the peak volume of cyclopentanone to L-carvone is 5.3-5.7, the origin is determined to be Evodia rutaecarpa; when the ratio is 6.2-7.6, the origin is determined to be Psoralea corylifolia. The headspace operation steps include: weighing 0.5-1 g of the powder of the Evodia rutaecarpa sample to be tested, placing it in a headspace injection bottle, incubating it at 80°C for 15 minutes, and then injecting the headspace sample.

2. The method according to claim 1, characterized in that The gas chromatography-ion mobility mass spectrometry conditions are as follows: chromatographic column: MXT-5, 30 μm, ID: 0.53 mm, df: 1.0 μm; chromatographic column temperature: 60°C; carrier gas / drift gas: N2; IMS temperature: 45°C; injection volume: 200 μL in the automatic headspace injection unit; incubation time: 15 min; incubation temperature: 80°C; injection needle temperature: 85°C; incubation speed: 500 rpm; The gas chromatography conditions were as follows: E1 drift gas volume flow rate was 75 mL / min; E2 gas phase carrier gas volume flow rate: 0-2 min, 2 mL / min; 2-10 min, 2-10 mL / min; 10-20 min, 10-100 mL / min; 20-25 min, 100-150 mL / min; 25-40 min, 150 mL / min.

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