Anti-acne cleansing formulations

Abstract

The invention provides an impregnant for decks.

Description

Technical Field

[0001] The present invention belongs to the fields of cosmetics and pharmaceuticals and relates to a cleansing formulation for treating acne and fungal acne. Background Art

[0002] The desire for beauty and attractiveness is an innate human trait. Although the ideals of beauty change over time, the pursuit of a flawless appearance has always been a goal, as a pleasing appearance enhances self-esteem and attractiveness to peers. Here, the condition and appearance of the skin carry significant weight in a beautiful and attractive appearance.

[0003] Oily and dirty skin is known as a transitional state between healthy normal skin and pathologically altered acne skin, in which the skin produces more sebum (seborrhea). This is an ideal breeding ground for many microorganisms, especially Propionibacterium acnes and Malassezia furfur. The microorganisms break down sebum into glycerol and fatty acids, thereby stimulating the sebaceous glands to increase production and attacking and destroying the hair follicle walls in the skin. This leads to inflammation in the skin (papules, pustules, nodules, cysts), which can usually only be cured with scarring, causing permanent damage to the visual appearance of people with dirty skin (W. Umbach [ed.], Kosmetik, Entwicklung, Herstellung und Anwendung kosmetischer Mittel (Cosmetics, Development, Production and Application of Cosmetics), 2nd edition, Thieme Verlag, Stuttgart, 1995).

[0004] Acne (acne vulgaris in the narrow sense) refers to various diseases of the sebaceous gland follicles, characterized by secretion and keratinization disorders, followed by inflammation and possible scarring. Acne vulgaris mainly occurs during puberty and is usually concentrated in skin areas rich in sebaceous glands (face, neck, chest, back). Hyperplasia of the sebaceous glands and keratinization disorders of the hair follicles lead to their blockage, forming comedones and the typical rashes for acne vulgaris (Pschyrembel, Clinical Dictionary, 258th edition, Walter de Gruyter Verlag, Berlin, 1998).

[0005] To prevent and treat acne, effective active ingredients are needed to reduce the colonization of microorganisms such as Propionibacterium acnes on the skin. Therefore, the active ingredients not only help improve the skin condition but also contribute to improving the health condition of the user.

[0006] Malassezia folliculitis, also known as Pityrosporum folliculitis, is an acne-like eruption on the human skin, usually accompanied by itching, and most commonly occurs in areas with high sebum activity. This mainly includes the face (especially the forehead), neck, shoulders, chest, and back.

[0007] Malassezia folliculitis is commonly known as fungal acne in colloquial language, even though this skin disease itself is not acne. The colloquial naming is only based on similar symptoms.

[0008] Malassezia folliculitis is a fungal disease that is often misdiagnosed as bacterial acne. This can be very frustrating for the people involved, because misdiagnosis often leads to unnecessary and long-term drug treatments, such as antibiotics, which only make the situation worse.

[0009] Another problematic situation is that in a large number of patients, a mixed form of bacterial acne and Malassezia folliculitis occurs. Unfortunately, there is currently no or very few active ingredient combinations that effectively reduce bacterial acne and Malassezia folliculitis.

[0010] Therefore, the object of the present invention is to provide a treatment possibility that is effective against bacterial acne and Malassezia folliculitis even in case of misdiagnosis.

[0011] Malassezia is a genus of yeasts or fungi that is a common component of the skin's microbiome and mycobiome (i.e., the healthy bacteria and yeasts living on the skin), estimated to be present in 92% of the human body. However, for reasons that are not yet conclusive, Malassezia is pathogenic to certain individuals and causes Malassezia folliculitis. In particular, it is believed that Malassezia folliculitis is caused by Malassezia yeast penetrating deep into the skin glands. This is usually accompanied by an increase in sebum secretion. As a result, skin irritations such as small papules, redness, and itching occur.

[0012] There are various known Malassezia yeasts. They are Malassezia ovina, Malassezia cuniculi, Malassezia cutaneous, Malassezia equina, Malassezia furfur, Malassezia globosa, Malassezia japonica, Malassezia nana, Malassezia obtusa, Malassezia pachydermatis, Malassezia restricta, Malassezia slooffiae, and Malassezia sympodialis. Malassezia folliculitis is mainly caused by Malassezia globosa and / or Malassezia furfur.

[0013] Doctors can prescribe different treatment methods for Malassezia folliculitis. In particular, treatment requires antifungal drugs. If the antifungal drug is taken orally, active substances such as itraconazole or fluconazole are used. However, these antifungal treatments are off-label because they are not approved by the FDA for this specific disease.

[0014] Alternatively, a large number of patients who do not want or cannot tolerate oral antifungal treatment try to locally treat Malassezia folliculitis with antifungal shampoos. Selenium sulfide shampoos, such as Head & Shoulders, Neutrogena, or pharmacy brands, can be purchased. The shampoos are usually easy to apply because they can be allowed to drip onto the chest, back, or shoulders after being applied to the neck or scalp.

[0015] The review literature available on the webpage https: / / simpleskincarescience.com / pityrosporum-folliculitis-treatment-malassezia-cure / lists various active substances that can be topically applied to pityrosporum folliculitis.

[0016] The most commonly used active ingredient is ketoconazole. Studies have shown that ketoconazole inhibits any type of Malassezia yeast, and its effectiveness is significantly better than other antifungal drugs. For example, its minimum inhibitory concentration (MHK) against Malassezia is lower than that of zinc pyrithione, selenium sulfide, clotrimazole, and bifonazole.

[0017] Various studies have investigated the effectiveness of ketoconazole in topical use:

[0018] - 44 patients (35 males and 9 females) were required to apply 2% ketoconazole cream twice a day or take oral itraconazole 100 mg. 37 patients using ketoconazole cream showed average improvement after 27 to 43 days. 7 patients taking oral itraconazole improved after 14 to 18 days. Therefore, topical application did not show rapid improvement. (105)

[0019] - 26 patients used topical ketoconazole, oral ketoconazole, or both. The cure rates were 12%, 75%, or 75%. Therefore, compared with oral use, topical application did not show a particularly high cure rate. (106)

[0020] Therefore, the efficacy of topical ketoconazole for pityrosporum folliculitis is limited.

[0021] It is also known that clotrimazole, benzoyl peroxide, salicylic acid, and zinc pyrithione are also used for the topical treatment of pityrosporum folliculitis.

[0022] Unfortunately, when topically applied to the skin, especially the facial skin, the individual active ingredients usually do not have sufficient effect on reducing Malassezia yeast. Therefore, pityrosporum folliculitis is not adequately controlled and the appearance of the skin is not improved.

[0023] The disadvantages of known active ingredients and combinations of known active ingredients also lie in that they usually cause skin irritation or skin dryness when used on the skin. Therefore, although pityrosporum folliculitis is treated, the skin condition does not improve significantly due to side effects. Therefore, there is a need for active ingredients and / or combinations of active ingredients that can achieve effective treatment but are significantly milder, so as to cause less or no skin irritation or skin dryness after application.

[0024] In addition, the disadvantage of active ingredients such as benzoyl peroxide is that they cause bleaching of textiles in contact with the skin after being applied to the skin.

[0025] As can be seen from the above, the success of the treatment of traditional acne caused and / or promoted by Propionibacterium acnes, and the success of the treatment of Malassezia folliculitis caused and / or promoted by Malassezia furfur depend on which active ingredients are used.

[0026] It is often found that these active substances have rather significant disadvantages. In addition, the effective reduction of these bacteria and yeasts can usually only be achieved by so-called "retention" treatments. This means that cosmetic or dermatological products containing active ingredients are applied to the skin and left there. However, rapid application is desired, i.e., the cosmetic or dermatological product is rinsed off the skin with water after a few minutes, and an effective reduction of Propionibacterium acnes and Malassezia furfur should be achieved. In addition, it is also desired to use no or fewer active ingredients harmful to the skin in such treatments. Summary of the Invention

[0027] Surprisingly, it is now possible to provide a cleansing preparation which, in conventional application, causes the death of Propionibacterium acnes and / or Malassezia furfur, and the reduction of which is achieved by the presence of specific surfactants.

[0028] Accordingly, the first subject of the present invention is a cosmetic or dermatological cleansing preparation which comprises

[0029] a) cocamidopropyl betaine; and

[0030] b) one or more substances selected from sodium laureth-6 carboxylate, capryloyl / hexanoyl methyl glucamide, and sodium lauroyl sarcosinate.

[0031] A preferred subject of the present invention is a cosmetic or dermatological cleansing preparation which comprises

[0032] a) cocamidopropyl betaine; and

[0033] b) sodium laureth-6 carboxylate.

[0034] A preferred subject of the present invention is a cosmetic or dermatological cleansing preparation which comprises

[0035] a) cocamidopropyl betaine; and

[0036] b) capryloyl / hexanoyl methyl glucamide.

[0037] A preferred subject of the present invention is a cosmetic or dermatological cleansing preparation which comprises

[0038] a) cocamidopropyl betaine; and

[0039] b) Sodium lauroyl sarcosinate.

[0040] If the term preparation is used hereinafter, unless otherwise stated, it always refers to the cleaning preparation according to the invention.

[0041] Another subject of the invention is the cosmetic use of the preparation according to the invention for reducing Propionibacterium acnes and / or Malassezia furfur on human skin.

[0042] Another subject of the invention is the preparation according to the invention for treating acne and / or Malassezia folliculitis on human skin.

[0043] Advantageously, it has been shown that the invention can also be particularly effective in treating conventional acne. Surprisingly, a combined product can be provided which can be used for both anti-acne and pityrosporum folliculitis. Therefore, the problem of misdiagnosis no longer exists, and thus the consumer or patient will obtain skin improvement in any case. Furthermore, it is particularly surprising that even with a particularly short action time of about 5 minutes on human skin, a particularly effective reduction of Propionibacterium acnes and / or Malassezia furfur can be achieved. After the action time, the preparation is rinsed with water.

[0044] Furthermore, it has surprisingly been found that the combination of active ingredients has a synergistic effect in acne and / or Malassezia folliculitis, respectively, in reducing Propionibacterium acnes and / or Malassezia furfur.

[0045] Unless otherwise stated, all tests and measurements are carried out under "normal conditions / standard conditions". The term "normal conditions / standard conditions" means 20 °C, 1013 hPa and 50% relative humidity.

[0046] The term "skin" only refers to human skin.

[0047] If the term "anti-Propionibacterium acnes" is used in the present disclosure, it means killing Propionibacterium acnes and / or reducing and / or preventing the growth and / or spread of the bacteria.

[0048] If the term "anti-Malassezia furfur" is used in the present disclosure, it means killing Malassezia furfur and / or reducing and / or preventing the growth and / or spread of Malassezia furfur.

[0049] If weight percentage data (wt%) are given hereinafter without mentioning a specific composition or a specific mixture, these data always refer to the total weight of the preparation. If the ratio of components / substances / groups of substances is disclosed hereinafter, these ratios refer to the weight ratio of the components / substances / groups of substances mentioned.

[0050] If a weight percentage range of the formulation components is given below, the disclosure of the present application also covers all individual values at 0.1 weight% intervals within these weight percentage ranges.

[0051] Expressions such as "according to the present invention", "advantageous according to the present invention", "advantageous in the sense of the present invention", etc. always refer to the formulations according to the present invention, the uses and methods according to the present invention.

[0052] In the sense of the present disclosure, the term "free of" means that the proportion of the substance does not exceed 0.1 weight%, especially 0 weight%, relative to the total weight of the formulation. Therefore, accidental introduction due to contamination should not result in exclusion from the scope of protection.

[0053] Emulsifiers refer to all substances listed under the name "emulsifier" in the International Cosmetic Ingredient Dictionary and Handbook, 13th Edition 2010 (ISBN 1-882621-47-6). Surfactants refer to all substances listed under the name "surfactant" in the International Cosmetic Ingredient Dictionary and Handbook, 13th Edition 2010 (ISBN 1-882621-47-6).

[0054] If viscosity values are given in the present disclosure, all values refer to the measurements taken at 25 °C in a 150 ml wide-neck bottle (VWR number: 807-0001) using a Rheomat R 123 from proRheo. The RheomatR 123 from proRheo GmbH is a rotational viscometer, i.e., the measuring body rotates in the substance to be measured. The measurement determines the force required to rotate the measuring body at a given speed in the sample. The viscosity is calculated from this torque, the rotational speed of the measuring body, and the geometric dimensions of the measuring system used. It is suitable for viscosity ranges up to 10,000 [mPa·s] and speed ranges from 62.5 min -1 of measuring body No. 1 (article number 2000191) is used as the measuring body.

[0055] If an average value is given in the present disclosure, the average value always refers to the arithmetic mean.

[0056] If molecular weight data of a polymer are given in the present disclosure, these data relate to measurements by light scattering.

[0057] Preferably, the formulation according to the present invention contains cocamidopropyl betaine in a proportion of 0.1 to 10 weight%, preferably 1.2 to 8 weight%, particularly preferably 2.8 to 5.5 weight%, respectively relative to the total weight of the formulation.

[0058] If the preparation contains sodium laureth-6 carboxylate, it is advantageous for the weight ratio of sodium laureth-6 carboxylate to cocamidobetaine to be from 10:1 to 1:10, preferably from 5:1 to 1:5, more preferably from 2:1 to 1:2, even more preferably from 1.5:1 to 1:1.5, and particularly preferably from 1.2:1 to 1:1.2.

[0059] If the preparation contains capryloyl / hexanoyl methyl glucamide, it is advantageous for the weight ratio of capryloyl / hexanoyl methyl glucamide to cocamidobetaine to be from 10:1 to 1:10, preferably from 5:1 to 1:5, more preferably from 2:1 to 1:2, even more preferably from 1.5:1 to 1:1.5, and particularly preferably from 1.2:1 to 1:1.2.

[0060] If the preparation contains sodium lauroyl sarcosinate, it is advantageous for the weight ratio of sodium lauroyl sarcosinate to cocamidobetaine to be from 10:1 to 1:10, preferably from 5:1 to 1:5, more preferably from 2:1 to 1:2, even more preferably from 1.5:1 to 1:1.5, and particularly preferably from 1.2:1 to 1:1.2.

[0061] If the preparation contains sodium laureth-6 carboxylate, capryloyl / hexanoyl methyl glucamide and / or sodium lauroyl sarcosinate, the total weight ratio of sodium laureth-6 carboxylate, capryloyl / hexanoyl methyl glucamide and sodium lauroyl sarcosinate to cocamidobetaine is from 10:1 to 1:10, preferably from 5:1 to 1:5, more preferably from 2:1 to 1:2, even more preferably from 1.5:1 to 1:1.5, and particularly preferably from 1.2:1 to 1:1.2.

[0062] Regarding the cosmetic preparation according to the invention, it is preferred that the proportion of sodium laureth-6 carboxylate is from 0.1 to 10% by weight, preferably from 1.2 to 8% by weight, and particularly preferably from 2.8 to 5.5% by weight, respectively relative to the total weight of the preparation.

[0063] Regarding the cosmetic preparation according to the invention, it is preferred to contain capryloyl / hexanoyl methyl glucamide in a proportion of from 0.1 to 10% by weight, preferably from 1.2 to 8% by weight, and particularly preferably from 2.8 to 5.5% by weight, respectively relative to the total weight of the preparation.

[0064] Regarding the cosmetic preparation according to the invention, it is preferred to contain sodium lauroyl sarcosinate in a proportion of from 0.1 to 10% by weight, preferably from 1.2 to 8% by weight, and particularly preferably from 2.8 to 5.5% by weight, respectively relative to the total weight of the preparation.

[0065] Regarding the cosmetic preparation according to the invention, it is preferred to contain sodium laureth-6 carboxylate, capryloyl / hexanoyl methyl glucamide and / or sodium lauroyl sarcosinate in a proportion of from 0.1 to 10% by weight, preferably from 1.2 to 8% by weight, and particularly preferably from 2.8 to 5.5% by weight, relative to the total weight of the preparation.

[0066] Furthermore, it is advantageous in the context of the present invention that the preparation contains at least one polylysine. As the name implies, polylysine is a homopolymer of the amino acid lysine. The precursor amino acid lysine contains two amino groups, one at the α-carbon and one at the ε-carbon. Both can be polymerization sites, giving rise to α-polylysine or ε-polylysine. Polylysine is a homopolypeptide in the group of cationic polymers: polylysine contains positively charged hydrophilic amino groups at pH 7.

[0067] It is advantageous according to the invention that ε-polylysine is included in the active substance combination according to the invention.

[0068] Regarding the preparation according to the invention, it is preferred that the proportion of polylysine is from 0.01 to 1% by weight, preferably from 0.1 to 0.5% by weight, particularly preferably from 0.15 to 0.3% by weight, respectively relative to the preparation according to the invention.

[0069] Regarding the preparation according to the invention, it is preferred according to the invention that the proportion of ε-polylysine is from 0.01 to 1% by weight, preferably from 0.1 to 0.5% by weight, particularly preferably from 0.15 to 0.3% by weight, respectively relative to the preparation according to the invention.

[0070] Furthermore, it is advantageous to include additives for further preservation of the preparation. Sodium benzoate is a typical such additive for cleaning preparations. Thus, it is preferred to contain sodium benzoate, where more preferably the total content of sodium benzoate is from 0.1 to 0.8% by weight relative to the total weight of the preparation.

[0071] A further preferred embodiment of the present invention is characterized by containing glycerol. If glycerol is contained, it is preferred that the proportion of glycerol is from 0.5 to 6% by weight, preferably from 1.5 to 4% by weight, relative to the total weight of the preparation.

[0072] Furthermore, it is advantageous that the pH value of the preparation is from 4.0 to 5.0, preferably from 4.2 to 4.8. The pH value is preferably adjusted by adding citric acid or sodium hydroxide.

[0073] Furthermore, it is advantageous that the preparation contains water as a cosmetic carrier, where the proportion of water is advantageously from 50 to 98% by weight, preferably from 60 to 90% by weight, relative to the total weight of the preparation.

[0074] Furthermore, it is preferred according to the invention that the preparation contains colorants. They allow for individual adjustment of the appearance of the preparation.

[0075] Advantageously, the preparation according to the present invention has a viscosity of at least 2000 mPa∙s. In this way, they can be stored in tubes with small outlets, or in dispensers or pump foaming devices and applied therefrom.

[0076] In addition, the preparations according to the invention may contain other additives and active ingredients, unless it is incompatible with the preparations according to the invention. Detailed Description

[0077] Examples:

[0078] The following examples are intended to illustrate the invention without limiting the invention to these examples. The values used in the examples are weight percentages relative to the total weight of each preparation.

[0079] Examples according to the invention are abbreviated as "Example X", where X is not consecutive numbers herein. The preparations represented by the following abbreviation "Comparative Example X" are comparative examples not according to the invention, where X is consecutive numbers.

[0080] Ingredient Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Water Add up to 100 Add up to 100 Add up to 100 Add up to 100 Add up to 100 Add up to 100 Glycerol Sodium benzoate 0.45 0.45 0.45 0.45 0.45 0.45 Citric acid 1 1 1 1 1 1 Mixture A 18.18 Mixture B 8 8 8 Mixture C 12.7 12.7 Mixture D 6.72 6.72 Mixture E 10 10 10 Mixture F 12.9 Mixture G ε-Polylysine 0.2 0.2 0.2 0.2 0.2 0.2 Suspension test results* Propionibacterium acnes 1 minute* 6.60E+05 6.63E+05 5.71E+05 1.64E+05 6.08E+05 7.06E+05 5 minutes* 6.40E+05 6.33E+05 5.95E+05 8.76E+02 4.54E+05 4.36E+05 Malassezia furfur 1 minute* 5.60E+05 5.63E+05 4.87E+05 5.16E+05 5.42E+05 5.56E+05 5 minutes* 5.41E+05 5.48E+05 4.93E+05 4.64E+05 5.28E+05 6.86E+05

[0081] *CFU data = Colony Forming Unit

[0082] Ingredient Example 7 Example 8 Example 9 Example 10 Example 11 Water Add up to 100 Add up to 100 Add up to 100 Add up to 100 Add up to 100 Glycerol 2.5 2.5 2.5 Sodium benzoate 0.45 0.45 0.45 0.45 Citric acid 1 1 1 1 1 Mixture A 18.18 18.18 Mixture B Mixture C 12.7 Mixture D Mixture E 23.5 23.5 Mixture F 12.9 29.84 Mixture G 13.33 13.33 13.33 ε-Polylysine 0.2 0.2 0.2 Suspension test results Propionibacterium acnes 1 minute* 1.07E+05 6.65E+05 1.49E+05 2.26E+05 2.64E+02 5 minutes* 2.92E+02 4.09E+05 5.95E+03 1.80E+04 1.55E+02 Malassezia furfur 1 minute* 5.36E+05 5.95E+05 5.61E+05 8.88E+04 8.53E+04 5 minutes* 6.66E+05 6.62E+05 2.60E+03 2.23E+03 5.34E+02

[0083] *CFU data = Colony Forming Unit

[0084] Ingredient Example 12 Example 13 Example 14 Example 15 Example 16 Control Water Add up to 100 Add up to 100 Add up to 100 Add up to 100 Add up to 100 Glycerol 2.5 2.5 2.5 2.5 2.5 Sodium benzoate 0.45 0.45 0.45 0.45 0.45 Citric acid 1 1 1 1 1 Mixture A Mixture B Mixture C Mixture D Mixture E Mixture F 25 20 29.84 25 20 Mixture G 13.33 13.33 13.33 13.33 13.33 ε-Polylysine 0.2 0.2 0.2 0.2 0.2 Suspension test results Propionibacterium acnes 1 minute* 1.85E+02 1.00E+02 1.82E+02 1.59E+02 1.03E+02 6.18E+06 5 minutes* 1.55E+02 1.03E+02 1.28E+02 4.22E+02 1.57E+02 5.68E+06 Malassezia furfur 1 minute* 2.49E+04 6.03E+04 1.07E+05 1.42E+05 1.07E+05 4.57E+05 5 minutes* 2.37E+02 1.34E+02 9.61E+02 6.14E+02 2.40E+02 4.30E+05

[0085] *CFU data = Colony Forming Unit

[0086] The pH value of all sample formulations is 4.5.

[0087] Mixture Ingredient. Weight%, relative to the total weight of each mixture Mixture A 22% Sodium laureth-6 carboxylate. 78% Water Mixture B 50% Octanoyl / hexanoyl methyl glucamide. 50% Water Mixture C 31.5% Sodium cocoamphoacetate. 7% Sodium chloride. 61.5% Water Mixture D 59.5% Octanoyl / hexanoyl glucoside. 40.2% Water. 0.3% Decanol Mixture E 40% Cocamidopropyl betaine. 54% Water. 6% Sodium chloride Mixture F 31% Coco-betaine. 62% Water. 7% Sodium chloride Mixture G 30% Sodium lauroyl sarcosinate. 70% Water. Appropriate amount of sodium chloride

[0088] Among the above example formulations, Example 4, Example 7 and Examples 11 to 16 are according to the invention. The results of the untreated control in the suspension test are also given in the above table (column: Control).

[0089] The preparations according to the invention show a surprising reduction in Propionibacterium acnes and / or Malassezia furfur in the suspension test. Other surfactant combinations do not result in a reduction in CFU.

[0090] The suspension test was carried out as follows:

[0091] Store the culture in glycerol created according to European Standard 12353 in a refrigerator at 5 °C. The first subculture was made from this liquid culture. Here, the culture was spread on the corresponding agar plate by aseptic single injection (see Table 1 for culture conditions). Incubate the culture (see the culture conditions table). Starting from this first subculture, a second subculture was then carried out, where some colonies were picked up with a sterile inoculation loop and then spread onto another new agar plate. Then the second generation was incubated at the corresponding temperature for 24 hours (see the culture conditions table). The obtained second generation can be used for the suspension test.

[0092] Table 1: Culture conditions

[0093] Culture Agar type Cultivation duration Cultivation temperature Culture medium Staphylococcus aureus Caso agar 24h 37°C Diluent Malassezia furfur Milk agar 5d 32°C SAB medium

[0094] The culture suspension was prepared as follows:

[0095] Using a sterile inoculation loop, pick up some colonies of the second generation and place them in 10 ml of medium in a Schikane flask containing 5 g of glass beads (Ø4 mm) (see Table 1 for culture conditions), and vortex (shake) for 3 minutes. Then roughly set the number of cells per milliliter by optical density (OD). Measure the optical density at 600 nm using an Eppendorf photometer. To eliminate the influence of the medium, perform a "blank" treatment with the medium used. Then measure the culture using the "sample" function. Now the obtained OD must be set to a value of around 0.2 (±0.01). The cell count generated by this dilution strategy is approximately 1x10 7 -1x10 8 .

[0096] To culture Propionibacterium acnes, pick up 2 - 3 colonies of the first generation and place them in a CELLSTAR CELL reactor tube filled with anaerobic medium. Vortex the tube in an anaerobic chamber with an anaerobic pad and incubate at 37 °C for at least 18 hours. The OD is also set to a value of around 0.2 (±0.01). For this purpose, anaerobic medium is used.

[0097] For testing, 50% / 500 µL of the preparation to be tested was diluted with 300 µL of water, and 100 µL of 3 g / L BSA was placed in a sterile Eppendorf reaction vessel. 800 µL of water and 100 µL of 3 g / L BSA were used as a positive control. In the test, double determinations were always carried out on individual components. After adding 100 µL of the separately produced culture suspension, the actual test was started. From this point in time, 1:10 dilutions (900 µL of neutralization medium + 100 µL of test sample) of all samples were prepared with the neutralization medium after 60 seconds and 300 seconds, respectively. A 1:1000 dilution (990 µL of neutralization medium + 10 µL of the 1:10 dilution) was prepared from this dilution. The diluted samples were spread on agar plates on a spiral plate (see Table 1 for culture conditions). After the test was completed, all agar plates were stored in an incubator for incubation and evaluated on a counting pad after incubation (see Table 1 for culture conditions). The CFU results are given in the table according to the examples.

Claims

1. A cleaning preparation, the cleaning preparation comprising: a) Cocamidobetaine; and b) One or more substances selected from sodium laureth-6 carboxylate, capryloyl / hexanoyl methyl glucamide, and sodium lauroyl sarcosinate.

2. The preparation according to claim 1, characterized in that it contains cocamidobetaine in a proportion of 0.1 to 10% by weight, preferably 1.2 to 8% by weight, particularly preferably 2.8 to 5.5% by weight, respectively relative to the total weight of the preparation.

3. The preparation according to any one of the preceding claims, characterized in that the preparation contains sodium laureth-6 carboxylate, wherein the weight ratio of sodium laureth-6 carboxylate to cocamidobetaine is 10:1 to 1:10, preferably 5:1 to 1:5, preferably 2:1 to 1:2, preferably 1.5:1 to 1:1.5, particularly preferably 1.2:1 to 1:1.

2.

4. The preparation according to any one of the preceding claims, characterized in that the preparation contains capryloyl / hexanoyl methyl glucamide, wherein the weight ratio of capryloyl / hexanoic acid methyl glucamide to cocamidobetaine is 10:1 to 1:10, preferably 5:1 to 1:5, preferably 2:1 to 1:2, preferably 1.5:1 to 1:1.5, particularly preferably 1.2:1 to 1:1.

2.

5. The preparation according to any one of the preceding claims, characterized in that the preparation contains sodium lauroyl sarcosinate, wherein the weight ratio of sodium lauroyl sarcosinate to cocamidobetaine is 10:1 to 1:10, preferably 5:1 to 1:5, preferably 2:1 to 1:2, preferably 1.5:1 to 1:1.5, particularly preferably 1.2:1 to 1:1.

2.

6. The preparation according to any one of the preceding claims, characterized in that the total weight ratio of sodium laureth-6 carboxylate, capryloyl / hexanoyl methyl glucamide, and sodium lauroyl sarcosinate to cocamidobetaine is 10:1 to 1:10, preferably 5:1 to 1:5, preferably 2:1 to 1:2, preferably 1.5:1 to 1:1.5, particularly preferably 1.2:1 to 1:1.

2.

7. The preparation according to any one of the preceding claims, characterized in that the preparation contains at least one polylysine.

8. The preparation according to any one of the preceding claims, characterized in that it contains ε-polylysine.

9. The preparation according to any one of the preceding claims, characterized in that it contains polylysine, and the proportion of polylysine is 0.01 to 1% by weight, preferably 0.1 to 0.5% by weight, particularly preferably 0.15 to 0.3% by weight, respectively relative to the preparation according to the present invention.

10. The preparation according to any one of the preceding claims, characterized in that it contains ε-polylysine, and the proportion of ε-polylysine is 0.01 to 1% by weight, preferably 0.1 to 0.5% by weight, particularly preferably 0.15 to 0.3% by weight, respectively relative to the preparation according to the present invention.

11. The preparation according to any one of the preceding claims, characterized in that the pH value of the preparation is 4.0 to 5.0, preferably 4.2 to 4.

8.

12. The preparation according to any one of the preceding claims, characterized in that the preparation comprises water as a cosmetic carrier, wherein the proportion of water is advantageously from 50 to 98% by weight, preferably from 60 to 90% by weight, relative to the total weight of the preparation.

13. The cosmetic use of the preparation according to any one of claims 1 to 12 for reducing Propionibacterium acnes and / or Malassezia furfur on human skin.

14. The preparation according to any one of claims 1 to 12 for treating acne and / or Malassezia folliculitis on human skin.