Colloidal solution material capable of promoting skin tissue repair as well as preparation method and application of colloidal solution material

Abstract

The invention belongs to the technical field of biological materials, and particularly relates to a colloidal solution material capable of promoting skin tissue repair and a preparation method and application thereof. The preparation method comprises the following steps: respectively pretreating silicon source powder, calcium source powder, phosphorus source powder and sodium source powder, respectively dissolving in matrix liquid, and centrifuging to obtain a stable solution; and uniformly stirring the obtained four solutions, adding a low-molecular polymer, uniformly stirring, and standing to obtain the colloidal solution material capable of promoting skin tissue repair. The obtained colloidal solution is uniform in material component, convenient for industrial preparation and high in biological safety, has the effects of promoting cell proliferation, repairing wounds, resisting inflammation and the like, and can provide a repairing carrier for repairing skins with different problems.

Description

Technical Field

[0001] The present invention belongs to the technical field of biomaterials, and particularly relates to a colloidal solution material capable of promoting skin tissue repair, a preparation method thereof, and an application thereof. Background Art

[0002] At present, due to the pressure of work, life, etc., humans often need to stay up late, resulting in disrupted work and rest schedules. This may further disrupt the body's biological clock, cause endocrine disorders, and then lead to problems such as weakened skin metabolism and decreased immunity. As a result, symptoms such as skin cutin shedding, skin sensitivity, skin dryness, itching, and redness may occur, leading to microtrauma on the skin. In addition, the skin may also be traumatized by some external stimuli, such as burns and ulcers. In addition to relying on the self-repair ability of the skin tissue itself for microtrauma or trauma on the skin, additional repair materials are needed to accelerate the healing and repair of the skin tissue to prevent problems such as bacterial infection caused by long-term unhealed trauma, which not only affects the beauty of the skin but also increases the mental and economic burden on patients. At the same time, there is also an inflammatory period during the skin repair process. Therefore, a material that can both anti-inflammatory and promote deep repair of skin tissue is needed to achieve perfect repair of traumatized skin and make the skin exist in a complete form. Summary of the Invention

[0003] In order to obtain a new type of repair biomaterial, the purpose of the present invention is to provide a colloidal solution material capable of promoting skin tissue repair for the repair of skin microtrauma or trauma, which can not only promote the proliferation of fibroblasts, but also show an inhibitory effect on the inflammatory response, and can accelerate the repair of the caudal fin of zebrafish after tail amputation.

[0004] The invention purpose of the present invention is achieved by the following technical solutions:

[0005] A colloidal solution material for promoting skin tissue repair provided by the present invention is obtained by multi-stage coupling of a silicon source, a calcium source, a phosphorus source, and a sodium source under the action of a matrix solution.

[0006] The present invention provides a preparation method of a colloidal solution material capable of promoting skin tissue repair, comprising the following steps:

[0007] (1) Pretreat the silicon source, calcium source, phosphorus source, and sodium source powders respectively, dissolve them in the matrix solution, and obtain a stable solution after centrifugation;

[0008] (2) Stir the silicon source solution, calcium source solution, phosphorus source solution, and sodium source solution obtained in step (1) evenly, add a low-molecular polymer and stir evenly, and then obtain the colloidal solution material capable of promoting skin tissue repair after standing.

[0009] Preferably, in step (1), the silicon source is one or more of hydrated silica, sodium metasilicate, silica, calcium silicate, and calcium sodium phosphosilicate; the calcium source is one or more of calcium phosphate, calcium silicate, calcium sodium phosphosilicate, and calcium pyrophosphate; the phosphorus source is one or more of calcium phosphate, calcium sodium phosphosilicate, calcium pyrophosphate, sodium metaphosphate, and sodium dihydrogen phosphate; the sodium source is one or more of calcium sodium phosphosilicate, sodium metaphosphate, and sodium dihydrogen phosphate.

[0010] Preferably, in step (1), the pretreatment method includes grinding and screening classification. The grinding time is 8 - 12 h, and the screening mesh number is 80 - 100 mesh.

[0011] Preferably, in step (1), the matrix solution is a mixed solution of deionized water and polyethylene glycol - 8 with a volume ratio of 80:20 - 95:5. Ultrasonic treatment is carried out during the dissolution process, and the ultrasonic treatment time is 20 - 60 min; the concentration of the silicon source in the matrix solution is 5 - 15 g / 100 mL; the concentration of the calcium source in the matrix solution is 0.005 - 0.1 g / 100 mL; the concentration of the phosphorus source in the matrix solution is 0.002 - 0.15 g / 100 mL; the concentration of the sodium source in the matrix solution is 0.001 - 0.005 g / 100 mL.

[0012] Preferably, in step (1), the centrifugation is gradient centrifugation. First, centrifuge at a speed of 300 - 500 r / min for 2 - 5 min, and then centrifuge at a speed of 800 - 1000 r / min for 2 - 5 min.

[0013] Preferably, in step (2), the mass ratio of the silicon source, calcium source, phosphorus source, and sodium source is (5 - 15):(0.005 - 0.1):(0.002 - 0.15):(0.001 - 0.005); the silicon source solution, calcium source solution, phosphorus source solution, and sodium source solution are added in sequence at time intervals of 20 ± 10 min for stirring. The stirring time is 2 - 8 h, the stirring speed is 200 - 240 r / min, and the stirring temperature is 50 - 60 °C.

[0014] Preferably, in step (2), the low - molecular polymer is one or more of polyethylene glycol - 8, hexanediol, and pentanediol, and its concentration in the mixed solution is 1 - 5 g / 100 mL; the stirring time after adding the low - molecular polymer is 1 - 2 h, and the stirring speed is 150 - 180 r / min.

[0015] The present invention also provides a colloidal solution material capable of promoting skin tissue repair prepared according to the above preparation method.

[0016] The present invention also provides an application of the above - mentioned colloidal solution material capable of promoting skin tissue repair in the preparation of products for skin wound repair.

[0017] The present invention also provides a carrier capable of promoting skin repair, comprising the above-mentioned colloidal solution material and an anti-inflammatory component, and the anti-inflammatory component is one or more of calendula extract and aloe extract.

[0018] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0019] 1. The components of the colloidal solution material of the present invention are uniform and present a colorless and transparent state, which is convenient for industrial preparation.

[0020] 2. The colloidal solution material of the present invention presents the Tyndall effect, which is a classic characterization method of colloidal solutions and can also illustrate the stability of the material system.

[0021] 3. The colloidal solution material of the present invention has high biosafety and is non-toxic and harmless to the human body.

[0022] 4. The colloidal solution material of the present invention can not only promote the proliferation of fibroblasts, but also accelerate the repair of the caudal fin of zebrafish with a severed tail, which can provide a great promotion for the repair of minimally invasive or traumatized skin tissues.

[0023] 5. The colloidal solution material of the present invention has an obvious inhibitory effect on the inflammation-related gene IL-1α. After adding a synergistic anti-inflammatory component (such as calendula extract, aloe, etc.), the anti-inflammatory effect of the colloidal solution material of the present invention can be significantly improved. When the colloidal solution is used alone, it has excellent anti-inflammatory and repair effects. When combined with other anti-inflammatory components, it can further enhance the anti-inflammatory and repair effects, which can provide a repair carrier for the repair of different problematic skin. Brief Description of the Drawings

[0024] Figure 1 It is the external view of the colloidal solution material prepared in Example 1 of the present invention.

[0025] Figure 2 It is the phenomenon presented by the solution materials prepared in Example 1 and the comparative example of the present invention under the irradiation of a laser pointer. The left figure is the comparative example, and the right figure is Example 1.

[0026] Figure 3 It is the cell viability diagram of the colloidal solution material prepared in Example 2 of the present invention.

[0027] Figure 4 It is the cell proliferation diagram of the colloidal solution material prepared in Example 2 of the present invention.

[0028] Figure 5 It is the zebrafish caudal fin repair diagram of the colloidal solution material prepared in Example 3 of the present invention.

[0029] Figure 6Graph showing the inhibition of the expression of the inflammation-related gene IL-1α by the colloidal solution material prepared in Example 3 of the present invention. Detailed implementation manners

[0030] The present invention will be further described in detail below in conjunction with examples and drawings, but the implementation manners of the present invention are not limited thereto.

[0031] In the examples of the present invention, those not specified in specific conditions are carried out according to conventional conditions or conditions recommended by the manufacturer. Raw materials, reagents, etc. not specified in the manufacturer are all conventional products that can be obtained through commercial purchase.

[0032] The present invention will be further described below in conjunction with specific examples and drawings. Unless otherwise specified, the following solution concentrations (%) are all ratios of the solute mass (g) to the solvent volume (mL):

[0033] Example 1

[0034] This example provides a preparation method of a colloidal solution material for promoting skin tissue repair, including the following steps:

[0035] Step 1: The components selected are hydrated silica (denoted as A), calcium silicate (denoted as B), calcium phosphate (denoted as C), and sodium dihydrogen phosphate (denoted as D);

[0036] Step 2: Grind the components in Step 2 for 8 h respectively;

[0037] Step 3: Screen the components in Step 2 with a 100-mesh sieve to obtain the sifted A, B, C, and D powders for use;

[0038] Step 4: Dissolve the A, B, C, and D powders in Step 3 in 100 mL of a matrix solution (a mixed solution of deionized water and polyethylene glycol-8 with a volume ratio of 85:15) respectively. The concentration of component A is 10%, the concentration of component B is 0.05%, the concentration of component C is 0.005%, and the concentration of component D is 0.002%. Ultrasonic for 30 min to obtain A1, B1, C1, and D1 solutions respectively;

[0039] Step 5: Centrifuge the A1, B1, C1, and D1 solutions in Step 4 at a speed of 400 r / min for 2 min first, and then centrifuge at a speed of 800 r / min for 2 min. Take the supernatant to obtain A2, B2, C2, and D2 solutions;

[0040] Step 6: Add the A2, B2, C2, and D2 solutions in Step 5 to the stirring device at 50 °C in sequence at 20-min intervals and stir evenly for 2 h to obtain E solution;

[0041] Step 7: Add 3% polyethylene glycol-8 low molecular polymer to the E solution in Step 6, stir for 1 h, and then obtain a stable colloidal solution after standing still.

[0042] Example 2

[0043] This example provides a preparation method of a colloidal solution material for promoting skin tissue repair, including the following steps:

[0044] Step 1: Select hydrated silica (denoted as A), calcium phosphate (denoted as B), and sodium dihydrogen phosphate (denoted as C) as components;

[0045] Step 2: Grind the components in Step 2 for 10 h respectively;

[0046] Step 3: Screen the components in Step 2 with a 100-mesh sieve to obtain the sifted A, B, and C powders for use;

[0047] Step 4: Dissolve the A, B, and C powders in Step 3 in 100 mL of a matrix solution (a mixed solution of deionized water and polyethylene glycol-8 with a volume ratio of 92:8) respectively. The concentration of component A is 12%, the concentration of component B is 0.1%, and the concentration of component C is 0.002%. Ultrasonic for 30 min to obtain A1, B1, and C1 solutions respectively;

[0048] Step 5: Centrifuge the A1, B1, and C1 solutions in Step 4 at a speed of 400 r / min for 3 min first, and then centrifuge at a speed of 900 r / min for 2 min to finally obtain A2, B2, and C2 solutions;

[0049] Step 6: Add the A2, B2, and C2 solutions in Step 5 to a stirring device at 60 °C at intervals of 20 min in sequence and stir evenly for 4 h to obtain an E solution;

[0050] Step 7: Add a mixed low molecular polymer of 2% polyethylene glycol-8, 1% pentanediol, and 0.2% hexanediol to the E solution in Step 6, stir for 2 h, and then obtain a stable colloidal solution after standing still.

[0051] Example 3

[0052] This example provides a preparation method of a colloidal solution material for promoting skin tissue repair, including the following steps:

[0053] Step 1: Select hydrated silica (denoted as A), calcium phosphate (denoted as B), calcium sodium phosphosilicate (denoted as C), and sodium dihydrogen phosphate (denoted as D) as components;

[0054] Step 2: Grind the components in Step 2 for 8 h respectively;

[0055] Step 3: Screen each component in Step 2 using a 100-mesh sieve to obtain the sifted powders of A, B, C, and D for later use;

[0056] Step 4: Add the powders of A, B, C, and D in Step 3 to 100 mL of a matrix solution (a mixture of deionized water and polyethylene glycol-8 with a volume ratio of 90:10) respectively. The concentration of component A is 14%, the concentration of component B is 0.08%, the concentration of component C is 0.003%, and the concentration of component D is 0.001%. Ultrasonic for 30 min to obtain solutions A1, B1, C1, and D1 respectively;

[0057] Step 5: Centrifuge the solutions A1, B1, C1, and D1 in Step 4 at a speed of 300 r / min for 3 min first, and then centrifuge at a speed of 900 r / min for 2 min to finally obtain solutions A2, B2, C2, and D2;

[0058] Step 6: Add the solutions A2, B2, C2, and D2 in Step 5 to a stirring device at 60 °C at intervals of 20 min in sequence and stir evenly for 6 h to obtain solution E;

[0059] Step 7: Add a mixture of 3% pentanediol and 0.5% hexanediol low molecular polymer to the solution E in Step 6 and stir for 2 h, and then obtain a stable colloidal solution after standing.

[0060] Comparative Example 1

[0061] This comparative example provides a preparation method of a solution material containing silicon, calcium, phosphorus, and sodium, including the following steps:

[0062] Step 1: Select components silicon dioxide (denoted as A), calcium chloride (denoted as B), calcium phosphate (denoted as C), and sodium carbonate (denoted as D);

[0063] Step 2: Grind the components in Step 2 for 8 h;

[0064] Step 3: Screen each component in Step 2 using a 100-mesh sieve to obtain the sifted powders of A, B, C, and D for later use;

[0065] Step 4: Add the powders of A, B, C, and D in Step 3 to 100 mL of a matrix solution (a mixture of deionized water and polyethylene glycol-8 with a volume ratio of 85:15). The proportion of component A is 10%, the proportion of component B is 0.05%, the proportion of component C is 0.005%, and the proportion of component D is 0.002%. Ultrasonic for 30 min to obtain solutions A1, B1, C1, and D1 respectively;

[0066] Step 5: Centrifuge the A1, B1, C1, and D1 solutions in Step 4 at a speed of 400 r / min for 2 min first, and then centrifuge at a speed of 800 r / min for 2 min. Finally, obtain the A2, B2, C2, and D2 solutions;

[0067] Step 6: Add the A2, B2, C2, and D2 solutions in Step 5 to a self-made stirring device at 50 °C at 20-min intervals in sequence and stir evenly for 2 h to obtain the E solution;

[0068] Step 7: Add 3% polyethylene glycol-8 low molecular polymer to the solution in Step 6 and stir for 1 h, and then obtain a stable colloidal solution after standing.

[0069] Test results:

[0070] Tyndall effect test: Take a bottle of the material in Example 1 (right figure) and a bottle of the material in Comparative Example 1 (left figure), use a black background as the photographing background, and irradiate the two bottles of materials from the left with infrared light (such as the laser of a page-turning pen). It can be seen from the results in the figure that the material in the example shows an obvious Tyndall effect, but the material in Comparative Example 1 does not show the Tyndall effect. Figure 2 The results show that the colloidal solution material prepared by the present invention shows the Tyndall effect, but the comparative example does not have this phenomenon.

[0071] Figure 3 、 4 In [reference numbers] and [reference numbers], experiments were carried out using fibroblasts. Figure 3 、 4 In [reference numbers] and [reference numbers], the control group refers to the blank control group that did not add the sample and only added PBS, and the experimental group added the colloidal solution material prepared in Example 2 of the present invention. Figure 3 It shows that the material prepared by the present invention is safe and non-toxic. Figure 4 It shows that the material prepared by the present invention has the effect of promoting cell proliferation.

[0072] Figure 5 In [reference numbers] and [reference numbers], the control group refers to the blank control group that did not add the sample and only added the liquid for raising fish, and the experimental group added the colloidal solution material prepared in Example 3 of the present invention. Figure 5 The results show that the material prepared by the present invention can accelerate the repair of the caudal fin of zebrafish after tail amputation.

[0073] During the skin wound repair process, there is an inflammatory phase. IL-1α is a typical inflammation-related gene. Therefore, it is very important to evaluate whether the material has an anti-inflammatory effect by evaluating the effect of the material on the expression of the IL-1α gene. The present invention uses macrophages RAW264.7 for experiments. Figure 6The control group in the experiment refers to the model group established by inflammation induction. In the experimental group, the colloidal solution material prepared in Example 3 of the present invention was added. In the experimental group with calendula extract added, the addition amount of calendula extract accounted for 0.6% of the sample solution. It can be clearly seen from Figure 6 the results that the prepared colloidal solution can significantly inhibit the expression of IL-1α gene, and especially the synergistic anti-inflammatory components can achieve a highly effective effect in inhibiting inflammation.

[0074] The above embodiments are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications made without departing from the spirit and principle of the present invention shall be equivalent replacement methods and are all included in the protection scope of the present invention.

Claims

1. A method for preparing a colloidal solution material that can promote skin tissue repair, characterized in that: The steps include: (1) Pre-treating silicon source, calcium source, phosphorus source, and sodium source powders, respectively, dissolving them in matrix liquid, and obtaining stable solutions after centrifugation; (2) The silicon source solution, calcium source solution, phosphorus source solution, and sodium source solution obtained in step (1) are stirred evenly, and a low molecular weight polymer is added and stirred evenly. After standing, the colloidal solution material capable of promoting skin tissue repair is obtained.

2. The preparation method according to claim 1, characterized in that: In step (2), the mass ratio of the silicon source, the calcium source, the phosphorus source, and the sodium source is (5-15): (0.005-0.1): (0.002-0.15): (0.001-0.005); And / or, in step (2), the low molecular weight polymer is one or more of polyethylene glycol-8, hexanediol, and pentanediol, and the concentration in the mixed solution is 1 to 5 g / 100 mL.

3. The preparation method according to claim 1 or 2, characterized in that: In step (1), the silicon source is one or more of hydrated silica, sodium metasilicate, silica, calcium silicate, and sodium calcium phosphosilicate; And / or, the calcium source is one or more of calcium phosphate, calcium silicate, sodium calcium phosphosilicate, and calcium pyrophosphate; And / or, the phosphorus source is one or more of calcium phosphate, sodium calcium phosphosilicate, calcium pyrophosphate, sodium metaphosphate, and sodium dihydrogen phosphate; And / or, the sodium source is one or more of sodium calcium phosphosilicate, sodium metaphosphate, and sodium dihydrogen phosphate; and / or, the concentration of the silicon source in the matrix liquid is 5 to 15 g / 100 mL; and / or, the concentration of the calcium source in the matrix solution is 0.005-0.1 g / 100 mL; and / or, the concentration of the phosphorus source in the matrix solution is 0.002 to 0.15 g / 100 mL; And / or, the concentration of the sodium source in the matrix solution is 0.001-0.005 g / 100 mL.

4. The preparation method according to claim 1 or 2, characterized in that: In step (1), the pretreatment method includes grinding and sieving and grading, the grinding time is 8 to 12 hours, and the sieving mesh size is 80 to 100 meshes.

5. The preparation method according to claim 1 or 2, characterized in that: In step (1), the matrix liquid is a mixed solution of deionized water and polyethylene glycol-8 in a volume ratio of 80:20 to 95:5, and ultrasonic treatment is performed during the dissolution process, and the ultrasonic treatment time is 20 to 60 minutes.

6. The preparation method according to claim 1 or 2, characterized in that: In step (1), the centrifugation is gradient centrifugation, first centrifuging at a speed of 300 to 500 r / min for 2 to 5 min, and then centrifuging at a speed of 800 to 1000 r / min for 2 to 5 min.

7. The preparation method according to claim 1 or 2, characterized in that: In step (2), the silicon source solution, calcium source solution, phosphorus source solution, and sodium source solution are added sequentially at a time interval of 20±10 min and stirred, the stirring time is 2 to 8 h, the stirring speed is 200 to 240 r / min, and the stirring temperature is 50 to 60° C.; And / or, the stirring time after adding the low molecular weight polymer is 1 to 2 hours and the stirring speed is 150 to 180 r / min.

8. A colloidal solution material capable of promoting skin tissue repair obtained by the preparation method according to any one of claims 1 to 7.

9. Use of the colloidal solution material capable of promoting skin tissue repair as claimed in claim 8 in the preparation of products for skin wound repair.

10. A carrier capable of promoting skin repair, characterized in that: The invention comprises the colloidal solution material according to claim 8 and an anti-inflammatory component, wherein the anti-inflammatory component is one or more of a marigold extract and an aloe extract.

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