Membrane separation equipment for polypeptide fractionation

Abstract

The invention discloses membrane separation equipment for polypeptide fractionation, and relates to the technical field of membrane separation, the membrane separation equipment comprises a separation mechanism, the separation mechanism comprises a separation cylinder and a plurality of separation membranes, and the separation membranes are longitudinally distributed in the separation cylinder; the vibration mechanism is arranged on the periphery of the separation membrane and comprises a piston cylinder and a piston block, the piston block is connected with a first traction rope and a second traction rope, the outer end of the first traction rope is connected to the center of the separation membrane and generates outward vibration waves in the center of the separation membrane, and the outer end of the second traction rope is connected with a knocking hammer; inward vibration waves are generated at the edge of the separation membrane, and the polypeptide liquid on the separation membrane is vibrated through the two vibration waves; the stirring mechanism is arranged above the separation membrane and comprises an impeller shaft, an impeller and stirring blades, the impeller and the stirring blades are installed at the upper end and the lower end of the impeller shaft respectively, the impeller is blown to rotate through airflow, and then the stirring blades are driven to stir polypeptide liquid on the separation membrane. The device has the advantages of being reasonable in scheme concept and ingenious in structural design.

Description

Technical Field

[0001] The present invention relates to the technical field of membrane separation, and particularly to a membrane separation device for polypeptide fractionation and separation. Background Art

[0002] The components of wastewater are complex and diverse. In addition to common organic and inorganic substances, it also contains a large number of potentially valuable components. Especially in biomass wastewater, there are abundant proteins, polypeptides and other organic molecules, which have high development and utilization value. As a natural bioactive substance, polypeptides have attracted much attention due to their wide applications in the fields of medicine, food, cosmetics, etc. Polypeptides not only have bioactive functions such as antibacterial, antioxidant and anti-inflammatory, but also can be used as drug carriers and added ingredients in functional foods. Therefore, how to efficiently extract and purify polypeptides from wastewater has become the focus of attention of researchers and enterprises.

[0003] At present, people have tried various methods for separating and extracting polypeptides from wastewater, such as solid-phase extraction, electrophoresis and precipitation methods. Although these traditional methods can extract polypeptides, they generally have problems such as complex operation, expensive equipment, low treatment efficiency and high energy consumption, and are difficult to meet the production requirements of industrial scale. In addition, in wastewater treatment, these methods are often accompanied by secondary pollution or resource waste, and the recovery rate is not high, which is not suitable for large-scale continuous treatment.

[0004] Compared with these traditional methods, membrane separation technology stands out with its advantages of high efficiency, energy saving and environmental protection. Membrane separation technology realizes the selective permeation and retention of substances through a physical separation process, and is widely used in the fields of water treatment, pharmaceutical separation and food processing. Especially nanofiltration membrane technology, which is between ultrafiltration membrane and reverse osmosis membrane, has unique separation characteristics, can efficiently retain organic molecules with molecular weights between 200 and 1000 daltons, and allows small molecule substances and water molecules to pass through. Therefore, the application of nanofiltration membrane in polypeptide separation and purification has great potential.

[0005] Based on the advantages of membrane separation technology and combined with the ultrafiltration-nanofiltration combined process, the ultrafiltration membrane is used to remove macromolecular impurities and suspended particles, while the nanofiltration membrane further enriches and purifies polypeptides, realizing the efficient combination of multi-stage filtration separation. This not only improves the purity and efficiency of polypeptide extraction, but also has significant energy-saving effects, is suitable for continuous production applications on an industrial scale, and provides a new solution for the recovery and utilization of valuable components in biomass wastewater.

[0006] In addition, since the membrane is a material with selective separation function, different components of the raw material liquid can be separated and purified through the different selective separation functions of different membranes. The process of separation through the membrane is called membrane separation. The membrane can separate the liquid containing protein molecules, effectively separate the protein molecules from the dissolved liquid, and make the protein adhere to the membrane, which is convenient for collection. However, when the membrane separation device separates macromolecular proteins for a long time, it is easy for a large amount of macromolecular proteins to adhere to one side of the membrane, blocking a large number of pores on the membrane surface through which water molecules can pass, thereby greatly reducing the water permeability of the membrane surface and resulting in a significant decrease in the flow rate of the liquid, affecting the separation speed of the membrane separation device. Summary of the Invention

[0007] The purpose of the present invention is to provide a membrane separation device for polypeptide fractional separation to solve the above-mentioned defects in the prior art.

[0008] A membrane separation device for polypeptide fractional separation includes a separation mechanism, a vibration mechanism and a stirring mechanism, wherein:

[0009] The separation mechanism includes a separation cylinder and a separation membrane. A plurality of separation membranes are provided and longitudinally distributed in the separation cylinder;

[0010] The vibration mechanism is arranged around the separation membrane and includes a piston cylinder and a piston block. The piston block is slidably connected in the piston cylinder. A first traction rope and a second traction rope are respectively connected to the piston block. The outer end of the first traction rope is connected to the center of the separation membrane, generating an outward vibration wave at the center of the separation membrane. The outer end of the second traction rope is connected to a hammer, generating an inward vibration wave at the edge of the separation membrane, and vibrating the polypeptide liquid on the separation membrane through the two vibration waves;

[0011] The stirring mechanism is arranged above the separation membrane and includes an impeller shaft, an impeller and a stirring blade. The impeller and the stirring blade are respectively installed at the upper and lower ends of the impeller shaft. The impeller is rotated by the blowing of air flow, thereby driving the stirring blade to stir the polypeptide liquid on the separation membrane.

[0012] Preferably, a maintenance port is provided on the side of the separation cylinder, and a maintenance door is hinged at the maintenance port. A feed hopper and a butterfly valve are respectively connected to the upper and lower ends of the separation cylinder. A tensioning frame and a hoop are respectively provided at the inner and outer edges of the separation membrane. A fixing ring is coaxially arranged outside the hoop and coaxially installed on the inner wall of the separation cylinder. A plurality of fixing bars are evenly connected to the fixing ring. A compression spring is connected between each fixing bar and the hoop. A circular sealing plate is coaxially connected to the inner side of the fixing ring. A sealing tube is coaxially connected to the center of the sealing plate, and a sealing ring is provided between the sealing tube and the tensioning frame.

[0013] Preferably, the piston cylinder is vertically installed on the hoop through a fixing plate. A sliding hole is provided at the center of the piston block, and an N-shaped sliding groove is continuously provided on the inner wall of the sliding hole. A sliding block is slidably connected in the sliding hole, and a plurality of sliding columns are uniformly fixed on the side surface of the sliding block. The sliding columns are correspondingly slidably connected in the sliding grooves. A motor is coaxially installed at the lower end of the piston cylinder, and the sliding block is coaxially installed at the output end of the motor. The upper and lower ends of the piston cylinder are respectively connected with a first pulley group and a second pulley group. The first traction rope is wound in a V shape around the first pulley group and the second pulley group, and its inner end is connected to the upper end of the piston block. The second traction rope is wound in a C shape around the first pulley group and the second pulley group, and its inner end is connected to the lower end of the piston block. An articulated seat is installed on the outer side of the piston cylinder, and an inverted L-shaped articulated frame is articulated on the articulated seat. The hammer is vertically fixed at the top of the articulated frame. The outer end of the second traction rope is connected to the upper part of the articulated frame.

[0014] Preferably, an impeller housing is coaxially provided outside the impeller. The impeller housing is coaxially installed in the sealing pipe through a fixing frame. A plurality of air inlet holes are evenly distributed on the side surface of the impeller housing. The impeller shaft and the stirring blades both adopt a hollow internal structure design. A plurality of spray holes are evenly provided at the lower edge of the stirring blades. The upper end of the piston cylinder is connected with an air inlet pipe, and the air inlet pipe is correspondingly communicated to the air inlet holes. A one-way valve is connected to each air inlet pipe. A plurality of air inlet holes are evenly distributed at the upper end of the piston cylinder, and a sealing piece is hinged inside the air inlet holes.

[0015] Preferably, a plurality of knocking blocks are evenly fixed on the outer side of the hoop, and the knocking blocks and the hammers correspond to each other one by one.

[0016] Preferably, a plurality of guide rods are evenly connected to the lower end of the piston block, and the guide rods are slidably connected to the lower end of the piston cylinder. A return spring is sleeved on each guide rod.

[0017] Preferably, a plurality of side suction holes are evenly provided in the middle of the impeller shaft, and a sealing ring is coaxially provided in the middle of the impeller shaft. A pair of side suction pipes are symmetrically connected to the left and right sides of the sealing ring, and the side suction pipes are installed on the fixing frame. The side suction pipes on both sides are respectively communicated with clear water and cleaning agent.

[0018] Preferably, there are two separation membranes, which are composed of an upper ultrafiltration membrane and a lower nanofiltration membrane.

[0019] Compared with the prior art, the present invention has the following advantages:

[0020] 1. The present invention relates to a purification method for efficiently extracting polypeptides from the wastewater of dead pigs. Based on membrane separation technology, it is particularly suitable for treating complex waste resources such as the wastewater of dead pigs and can efficiently purify polypeptides with high purity. This method has the advantages of simple process, convenient operation, high purification efficiency, etc., and is particularly suitable for large-scale industrial applications. By utilizing the different separation characteristics of ultrafiltration membranes and nanofiltration membranes, it can effectively remove macromolecular impurities in the wastewater and concentrate the polypeptide content, and finally obtain polypeptides with a purity of up to 99.9%.

[0021] 2. During the membrane separation of the polypeptide solution, the piston block is driven by a motor to slide up and down. When the piston block teleports, the elastic potential energy stored when the elastic traction rope one is tightened is quickly transferred to the center of the taut separation membrane when it relaxes, and a vibration wave from the inside to the outside is generated at the center of the separation membrane. The non-elastic traction rope two is used to drive the hammer to collide with the hoop, and the power of the hammer is transmitted to the edge of the taut separation membrane, and a vibration wave from the outside to the inside is generated at the edge of the separation membrane. And the polypeptide liquid on the separation membrane is vibrated by the two vibration waves to disperse the macromolecular proteins accumulated on the upper surface of the separation membrane.

[0022] 3. During the membrane separation of the polypeptide solution, the piston block is driven by a motor to slide up and down. When the piston block teleports, the air in the upper part of the piston cylinder is quickly squeezed into the impeller housing through the air inlet pipe, and the impeller in the impeller housing is pushed to rotate, thereby driving the stirring blade to stir the polypeptide liquid on the separation membrane, crushing the macromolecular proteins accumulated on the upper surface of the separation membrane. The air after doing work then enters the hollow stirring blade through the hollow impeller shaft and is finally evenly sprayed into the polypeptide liquid through the spray holes to stir evenly the macromolecular proteins accumulated on the upper surface of the separation membrane. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is a three-dimensional structure schematic diagram of the whole of the present invention.

[0024] Figure 2 It is a three-dimensional structure schematic diagram of the whole separation mechanism.

[0025] Figure 3 It is a three-dimensional structure schematic diagram of a partial view of the separation mechanism from the first perspective.

[0026] Figure 4 It is a three-dimensional structure schematic diagram of a partial view of the separation mechanism from the second perspective.

[0027] Figure 5 It is a schematic diagram of the sectional view of the partial separation mechanism.

[0028] Figure 6 It is a three-dimensional structure schematic diagram of the whole vibration mechanism.

[0029] Figure 7It is a schematic three-dimensional structure diagram of a part of the vibration mechanism.

[0030] Figure 8 It is a schematic sectional structure diagram of a part of the vibration mechanism.

[0031] Figure 9 It is a schematic exploded structure diagram of a part of the vibration mechanism.

[0032] Figure 10 It is a schematic three-dimensional structure diagram of the whole stirring mechanism.

[0033] Figure 11 It is a schematic three-dimensional structure diagram of a part of the stirring mechanism.

[0034] Wherein:

[0035] 10 - Separation mechanism; 101 - Separation cylinder; 101a - Maintenance opening; 102 - Maintenance door; 103 - Feed hopper; 104 - Butterfly valve; 105 - Separation membrane; 106 - Tensioning frame; 107 - Hoop; 108 - Knocking block; 109 - Fixed ring; 110 - Fixed strip; 111 - Compression spring; 112 - Sealing plate; 113 - Sealing pipe; 114 - Sealing ring;

[0036] 20 - Vibration mechanism; 201 - Piston cylinder; 201a - Air inlet hole; 202 - Sealing piece; 203 - Fixed plate; 204 - Piston block; 204a - Sliding hole; 204b - Sliding groove; 205 - Sliding block; 206 - Sliding column; 207 - Motor; 208 - Guide rod; 209 - Return spring; 210 - Pulley set one; 211 - Pulley set two; 212 - Pulling rope one; 213 - Pulling rope two; 214 - Hinge seat; 215 - Hinge frame; 216 - Knocking hammer;

[0037] 30 - Stirring mechanism; 301 - Impeller housing; 301a - Air inlet hole; 302 - Fixed frame; 303 - Impeller shaft; 303a - Side suction hole; 304 - Impeller; 305 - Stirring blade; 305a - Injection hole; 306 - Air inlet pipe; 307 - Check valve; 308 - Sealing ring; 309 - Side suction pipe. Specific embodiments

[0038] In order to make the technical means, creative features, achieved purposes and effects of the present invention easy to understand, the present invention will be further described below in conjunction with specific embodiments.

[0039] As Figures 1 to 11 shown, a membrane separation device for polypeptide fractionation includes a separation mechanism 10, a vibration mechanism 20 and a stirring mechanism 30, wherein:

[0040] The separation mechanism 10 includes a separation cylinder 101 and a separation membrane 105. A plurality of the separation membranes 105 are provided and longitudinally distributed in the separation cylinder 101;

[0041] The vibration mechanism 20 is arranged around the separation membrane 105 and includes a piston cylinder 201 and a piston block 204. The piston block 204 is slidably connected in the piston cylinder 201. A first traction rope 212 and a second traction rope 213 are respectively connected to the piston block 204. The outer end of the first traction rope 212 is connected to the center of the separation membrane 105, generating an outward vibration wave at the center of the separation membrane 105. The outer end of the second traction rope 213 is connected to a hammer 216, generating an inward vibration wave at the edge of the separation membrane 105, and vibrating the polypeptide liquid on the separation membrane 105 through the two vibration waves;

[0042] The stirring mechanism 30 is arranged above the separation membrane 105 and includes an impeller shaft 303, an impeller 304 and a stirring blade 305. The impeller 304 and the stirring blade 305 are respectively installed at the upper and lower ends of the impeller shaft 303. The impeller 304 is rotated by air flow, and then the stirring blade 305 is driven to stir the polypeptide liquid on the separation membrane 105.

[0043] In this embodiment, a maintenance port 101a is provided on the side of the separation cylinder 101, and a maintenance door 102 is hingedly connected at the maintenance port 101a. A feed hopper 103 and a butterfly valve 104 are respectively connected to the upper and lower ends of the separation cylinder 101. A tension frame 106 and a hoop 107 are respectively provided at the inner and outer edges of the separation membrane 105. A fixing ring 109 is coaxially provided outside the hoop 107, and the fixing ring 109 is coaxially installed on the inner wall of the separation cylinder 101. A plurality of fixing bars 110 are evenly connected to the fixing ring 109. A compression spring 111 is connected between each fixing bar 110 and the hoop 107. A circular sealing plate 112 is coaxially connected to the inner side of the fixing ring 109. A sealing tube 113 is coaxially connected to the center of the sealing plate 112, and a sealing ring 114 is provided between the sealing tube 113 and the tension frame 106. Both nanofiltration membranes and ultrafiltration membranes require a certain pressure to be applied during filtration to drive the liquid through the membrane pores to complete the separation process. The above sealing structure can apply a certain pressure to the solution to be filtered. This pressure is called the operating pressure or transmembrane pressure (TMP), which is crucial for driving the solution through the membrane pores for separation. The polypeptide solution enters the separation cylinder 101 through the feed hopper 103, and then the polypeptide solution is fractionally separated by multiple separation membranes 105, and finally the separated water is discharged outside the separation cylinder 101 through the butterfly valve 104.

[0044] In this embodiment, the piston cylinder 201 is vertically installed on the hoop 107 through the fixed plate 203. A sliding hole 204a is provided at the center of the piston block 204, and an N-shaped sliding groove 204b is continuously provided on the inner wall of the sliding hole 204a. A sliding block 205 is slidably connected in the sliding hole 204a, and a plurality of sliding columns 206 are uniformly fixed on the side surface of the sliding block 205. The sliding columns 206 are correspondingly slidably connected in the sliding grooves 204b. A motor 207 is coaxially installed at the lower end of the piston cylinder 201, and the sliding block 205 is coaxially installed at the output end of the motor 207. The upper and lower ends of the piston cylinder 201 are respectively connected with a first pulley group 210 and a second pulley group 211. The first traction rope 212 is wound around the first pulley group 210 and the second pulley group 211 in a V shape, and its inner end is connected to the upper end of the piston block 204. The second traction rope 213 is wound around the first pulley group 210 and the second pulley group 211 in a C shape, and its inner end is connected to the lower end of the piston block 204. An articulated seat 214 is installed on the outer side of the piston cylinder 201, and an inverted L-shaped articulated frame 215 is articulated on the articulated seat 214. The hammer 216 is vertically fixed at the top of the articulated frame 215. The outer end of the second traction rope 213 is connected to the upper part of the articulated frame 215. The motor 207 drives the sliding columns 206 on the sliding block 205 to rotate. At the same time, the sliding columns 206 slide along the N-shaped sliding grooves 204b. When the sliding columns 206 slide on the spiral section of the sliding grooves 204b, the piston block 204 can be driven to move downward. When the sliding columns 206 slide on the straight section of the sliding grooves 204b, the piston block 204 can quickly move upward to the initial height under the action of the pressure difference between the upper and lower sides. When the piston block 204 teleports, the elastic potential energy stored when the elastic first traction rope 212 is tensioned is quickly transmitted to the center of the taut separation membrane 105 when it is relaxed, and a vibration wave from the inside to the outside is generated at the center of the separation membrane 105. The non-elastic second traction rope 213 drives the hammer 216 to collide with the hoop 107, and the power of the hammer 216 is transmitted to the edge of the taut separation membrane 105, and a vibration wave from the outside to the inside is generated at the edge of the separation membrane 105. And the polypeptide liquid on the separation membrane 105 is vibrated by the two vibration waves, and the macromolecular proteins accumulated on the upper surface of the separation membrane 105 are dispersed.

[0045] In this embodiment, an impeller housing 301 is coaxially arranged outside the impeller 304. The impeller housing 301 is coaxially installed in the sealing tube 113 through a fixing bracket 302. A plurality of air inlet holes 301a are evenly distributed on the side surface of the impeller housing 301. Both the impeller shaft 303 and the stirring blade 305 adopt a hollow internal structure design. A plurality of injection holes 305a are evenly arranged at the lower edge of the stirring blade 305. The upper end of the piston cylinder 201 is connected with an air inlet pipe 306, and the air inlet pipe 306 is correspondingly communicated with the air inlet holes 301a. A one-way valve 307 is connected to each air inlet pipe 306. A plurality of air inlet holes 201a are evenly distributed at the upper end of the piston cylinder 201, and a sealing piece 202 is hinged inside the air inlet holes 201a. When the piston block 205 slowly slides downward in the piston cylinder 201, since the sealing piece 202 is open at the air inlet holes 201a, external air can be inhaled into the upper part of the piston cylinder 101 through the air inlet holes 301a; when the piston block 205 quickly slides upward in the piston cylinder 201, since the sealing piece 202 seals the air inlet holes 201a, the air in the upper part of the piston cylinder 101 is quickly squeezed into the impeller housing 301 through the air inlet pipe 306, and the impeller 304 in the impeller housing 301 is pushed to rotate, thereby driving the stirring blade 305 to stir the polypeptide liquid on the separation membrane 105, crushing the macromolecular proteins accumulated on the upper surface of the separation membrane 105, and the air after doing work enters the hollow stirring blade 305 through the hollow impeller shaft 303, and finally is evenly sprayed into the polypeptide liquid through the injection holes 305a to stir the macromolecular proteins accumulated on the upper surface of the separation membrane 105.

[0046] In this embodiment, a plurality of knocking blocks 108 are evenly fixed on the outer side of the hoop 107, and the knocking blocks 108 and the knocking hammers 216 correspond to each other one by one. By colliding the knocking hammers 216 with the knocking blocks 108 on the hoop 107, it is avoided that the hoop 107 is deformed by being hit by the knocking hammers 216 after long-term collision.

[0047] In this embodiment, a plurality of guide rods 208 are evenly connected to the lower end of the piston block 204, and the guide rods 208 are slidably connected with the lower end of the piston cylinder 201. A return spring 209 is sleeved on each guide rod 208. The guide rods 208 can prevent the piston block 205 from deflecting when sliding in the piston cylinder 201, and the return spring 209 can help the piston block 205 quickly move upward to the initial height.

[0048] In this embodiment, several side suction holes 303a are evenly arranged in the middle of the impeller shaft 303, and a sealing ring 308 is coaxially arranged in the middle of the impeller shaft 303. A pair of side suction pipes 309 are symmetrically connected to the left and right sides of the sealing ring 308, and the side suction pipes 309 are installed on the fixing frame 302. The side suction pipes 309 on both sides are respectively connected to clean water and detergent. When high-speed air flows through the inside of the hollow impeller shaft 303, due to the Venturi effect at the side suction pipes 309, clean water or detergent can be sucked into the hollow stirring blade 305 together, and finally evenly sprayed into the polypeptide liquid through the spray holes 305a. When the separation membrane 105 is in use, clean water can appropriately dilute the polypeptide solution. After the separation membrane 105 is used, clean water and detergent can effectively clean the separation membrane 105.

[0049] In this embodiment, there are two separation membranes 105, which consist of an upper ultrafiltration membrane and a lower nanofiltration membrane. The ultrafiltration membrane is used to remove macromolecular impurities and suspended particulate matters, while the nanofiltration membrane further enriches and purifies polypeptides, achieving an efficient combination of multi-stage filtration and separation.

[0050] The working principle of this membrane separation device for polypeptide fractionation:

[0051] The polypeptide solution enters the separation cylinder 101 through the feed hopper 103, and then the polypeptide solution is fractionated by multiple separation membranes 105. Finally, the separated water is discharged out of the separation cylinder 101 through the butterfly valve 104.

[0052] During the membrane separation of the polypeptide solution, the sliding column 206 on the sliding block 205 is driven to rotate by the motor 207. At the same time, the sliding column 206 slides along the spiral section of the N-shaped sliding groove 204b. When the sliding column 206 slides on the spiral section of the sliding groove 204b, the piston block 204 can be driven to move downward. When the sliding column 206 slides on the straight section of the sliding groove 204b, the piston block 204 can quickly move upward to the initial height under the action of the upper and lower pressure differences. When the piston block 204 teleports, the elastic potential energy stored when the elastic traction rope one 212 is tightened is quickly transmitted to the center of the taut separation membrane 105 when it is relaxed, and a vibration wave from the inside to the outside is generated at the center of the separation membrane 105. The non-elastic traction rope two 213 is used to drive the hammer 216 to collide with the hoop 107, and the power of the hammer 216 is transmitted to the edge of the taut separation membrane 105, and a vibration wave from the outside to the inside is generated at the edge of the separation membrane 105. And the polypeptide liquid on the separation membrane 105 is vibrated by the two vibration waves to disperse the macromolecular proteins accumulated on the upper surface of the separation membrane 105.

[0053] During the membrane separation of the polypeptide solution, when the piston block 205 slowly slides downward in the piston cylinder 201, since the sealing piece 202 is open at the air inlet hole 201a, external air can be inhaled into the upper part of the piston cylinder 101 through the air inlet hole 301a; when the piston block 205 quickly slides upward in the piston cylinder 201, since the sealing piece 202 seals the air inlet hole 201a, the air in the upper part of the piston cylinder 101 is quickly squeezed into the impeller housing 301 through the air inlet pipe 306, and the impeller 304 in the impeller housing 301 is pushed to rotate, thereby driving the stirring blade 305 to stir the polypeptide liquid on the separation membrane 105, crushing the macromolecular proteins accumulated on the upper surface of the separation membrane 105, and the air after doing work enters the hollow stirring blade 305 through the hollow impeller shaft 303, and finally is evenly sprayed into the polypeptide liquid through the spray holes 305a to stir the macromolecular proteins accumulated on the upper surface of the separation membrane 105.

[0054] Therefore, the above-disclosed embodiments are illustrative in all respects and not exclusive. All changes within the scope of the present invention or within the scope equivalent to the present invention are encompassed by the present invention.

Claims

1. A membrane separation device for polypeptide fractionation, characterized in that: It comprises a separation mechanism (10), a vibration mechanism (20) and a stirring mechanism (30), wherein: The separation mechanism (10) comprises a separation cylinder (101) and a separation membrane (105); a plurality of separation membranes (105) are provided and longitudinally distributed in the separation cylinder (101); The vibration mechanism (20) is arranged around the separation membrane (105) and includes a piston cylinder (201) and a piston block (204). The piston block (204) is slidably connected to the piston cylinder (201). A traction rope 1 (212) and a traction rope 2 (213) are respectively connected to the piston block (204). The outer end of the traction rope 1 (212) is connected to the center of the separation membrane (105) and generates an outward vibration wave at the center of the separation membrane (105). The outer end of the traction rope 2 (213) is connected to a hammer (216) and generates an inward vibration wave at the edge of the separation membrane (105). The polypeptide liquid on the separation membrane (105) is vibrated by the two vibration waves. The stirring mechanism (30) is arranged above the separation membrane (105) and includes an impeller shaft (303), an impeller (304) and a stirring blade (305). The impeller (304) and the stirring blade (305) are respectively installed at the upper and lower ends of the impeller shaft (303). The impeller (304) is driven to rotate by airflow, thereby driving the stirring blade (305) to stir the polypeptide liquid on the separation membrane (105).

2. A membrane separation device for polypeptide fractionation according to claim 1, characterized in that: The side of the separation cylinder (101) is provided with an inspection port (101a), and an inspection door (102) is hingedly connected to the inspection port (101a). The upper and lower ends of the separation cylinder (101) are respectively connected to a feed hopper (103) and a butterfly valve (104). The inner and outer edges of the separation membrane (105) are respectively provided with a tension frame (106) and a hoop (107). The outer periphery of the hoop (107) is coaxially provided with a fixing ring (109), and the fixing ring (109) is coaxially installed on On the inner wall of the separation cylinder (101), a plurality of fixing strips (110) are evenly connected to the fixing ring (109), a compression spring (111) is connected between each fixing strip (110) and the hoop (107), a circular sealing plate (112) is coaxially connected to the inner side of the fixing ring (109), a sealing tube (113) is coaxially connected to the center of the sealing plate (112), and a sealing ring (114) is provided between the sealing tube (113) and the tension frame (106).

3. The membrane separation device for polypeptide fractionation according to claim 2, characterized in that: The piston cylinder (201) is vertically mounted on the hoop (107) through a fixing plate (203); a sliding hole (204a) is provided at the center of the piston block (204); and N-shaped sliding grooves (204b) are continuously provided on the hole wall of the sliding hole (204a); a sliding block (205) is slidably connected in the sliding hole (204a); and a plurality of sliding columns (206) are evenly fixed on the side of the sliding block (205); and the sliding columns (206) are correspondingly slidably connected in the sliding grooves (204b); a motor (207) is coaxially mounted on the lower end of the piston cylinder (201); and the sliding block (205) is coaxially mounted on the output end of the motor (207); and the upper and lower ends of the piston cylinder (201) are respectively The piston cylinder (201) is respectively connected to a pulley block 1 (210) and a pulley block 2 (211); the traction rope 1 (212) is wound around the pulley block 1 (210) and the pulley block 2 (211) in a V shape, and its inner end is connected to the upper end of the piston block (204); the traction rope 2 (213) is wound around the pulley block 1 (210) and the pulley block 2 (211) in a C shape, and its inner end is connected to the lower end of the piston block (204); an articulated seat (214) is installed on the outer side of the piston cylinder (201), and an inverted L-shaped articulated frame (215) is articulated on the articulated seat (214); the percussion hammer (216) is vertically fixed to the top of the articulated frame (215), and the outer end of the traction rope 2 (213) is connected to the upper part of the articulated frame (215).

4. The membrane separation device for polypeptide fractionation according to claim 2, characterized in that: An impeller shell (301) is coaxially arranged on the outside of the impeller (304). The impeller shell (301) is coaxially installed in the sealing tube (113) through a fixing frame (302). A plurality of air inlet holes (301a) are evenly distributed on the side of the impeller shell (301). The impeller shaft (303) and the stirring blade (305) are both designed with an internal hollow structure. A plurality of injection holes (305a) are evenly arranged on the lower edge of the stirring blade (305). An air inlet pipe (306) is connected to the upper end of the piston cylinder (201), and the air inlet pipe (306) is correspondingly connected to the air inlet hole (301a). A one-way valve (307) is connected to each air inlet pipe (306). A plurality of air inlet holes (201a) are evenly distributed on the upper end of the piston cylinder (201), and a sealing sheet (202) is hinged on the inner side of the air inlet hole (201a).

5. The membrane separation device for polypeptide fractionation according to claim 3, characterized in that: A plurality of striking blocks (108) are evenly fixed on the outer side of the hoop (107), and the striking blocks (108) correspond to the striking hammers (216) one by one.

6. The membrane separation device for polypeptide fractionation according to claim 3, characterized in that: The lower end of the piston block (204) is evenly connected to a plurality of guide rods (208), and the guide rods (208) are slidably connected to the lower end of the piston cylinder (201), and each guide rod (208) is sleeved with a return spring (209).

7. The membrane separation device for polypeptide fractionation according to claim 4, characterized in that: A plurality of side suction holes (303a) are evenly arranged in the middle of the impeller shaft (303), and a sealing ring (308) is coaxially arranged in the middle of the impeller shaft (303). A pair of side suction pipes (309) are symmetrically connected to the left and right sides of the sealing ring (308), and the side suction pipes (309) are installed on the fixing frame (302). The side suction pipes (309) on both sides are connected to clean water and detergent respectively.

8. A membrane separation device for polypeptide fractionation according to any one of claims 1 to 7, characterized in that: The separation membrane (105) is provided with two membranes, which are composed of an upper ultrafiltration membrane and a lower nanofiltration membrane.

Images