Cell suspension preparation device

Abstract

The invention discloses a cell suspension preparation device and belongs to the technical field of cell suspension preparation.The cell suspension preparation device is provided with a centrifugal machine shell, a first connecting column, a second connecting column, a placement frame and a pressing cover, and the placement frame is correspondingly clamped to one surface of a limiting clamping strip till the placement frame is inserted into a placement groove; after test tubes with covers are oppositely inserted into test tube grooves in pairs, pressing covers are clamped on the surfaces of second connecting columns, so that the pressing covers are tightly attached to the top of the placement frame, and it is ensured that fixed pressing grooves in the surfaces of the pressing covers are clamped at the top ends of the test tubes with the covers; meanwhile, a second limiting clamping groove in a pressing cover is clamped on the surface of a corresponding second limiting sliding strip, a fixing screw sleeve is tightened to be tightly attached to the top of the pressing cover, and the pressing cover is fixed to the top of the containing frame, so that the containing frames, the test tubes with the covers and the pressing cover of different specifications can be conveniently disassembled and assembled according to the cell suspension preparation requirements.

Description

Technical Field

[0001] The present invention relates to the technical field of cell suspension preparation, and particularly to a cell suspension preparation device. Background Art

[0002] In the field of modern bioscience, cell research occupies an extremely important position. Whether it is basic cell biology research, such as exploring the physiological mechanisms of cells and cell signal transduction, or in application fields such as medicine and pharmaceuticals, like cell therapy and drug screening, the processing of cells is indispensable. The preparation of cell suspension is a crucial starting step for many cell-related research and applications. In order to facilitate the precise analysis of gene expression, protein expression, etc. of cells and reveal the heterogeneity and functional differences between cells, it is necessary to disperse cells into a suspension. At the same time, the cells cultured in suspension can be evenly distributed in the liquid medium, which is beneficial to the growth and proliferation of cells and is convenient for large-scale cell culture and production. During the preparation of cell suspension, when dissociating cells from tissue samples, cell debris and undigested tissue residues will be generated. By using a centrifuge, these heavier impurities can be precipitated to the bottom of the test tube, while the cells are suspended in the supernatant, thus realizing the separation of cells from impurities and facilitating the collection of cells for subsequent experimental operations, such as cell culture, cell counting, cell analysis, etc.

[0003] When the existing cell suspension preparation device fixes the test tube containing the cell stock solution inside the placement rack, due to different amounts of cell stock solution required according to experimental needs, test tubes of different specifications and sizes are used to hold the cell stock solution. Since the placement rack of a general centrifuge is directly fixed inside the centrifuge, it is difficult to arbitrarily replace the corresponding placement rack according to the different specifications and sizes of the test tubes. When centrifuging a large number of cell stock solutions, it is necessary to load them into test tubes in batches for centrifugation operations, which requires multiple centrifugation operations and affects the preparation efficiency of cell suspension. At the same time, the cover of the centrifuge is generally opened or closed by manual flipping. Since the cover is heavy, a large amount of force is required to flip it open, and the operation may be laborious. Especially for application scenarios that require frequent opening and closing, it may affect the work efficiency. Therefore, improvements are needed.

[0004] Based on this, the present invention designs a cell suspension preparation device to solve the above problems. Summary of the Invention

[0005] The object of the present invention is to provide a device for preparing cell suspension, so as to solve the problems proposed in the above-mentioned background technology. When the test tube containing the original cell solution is fixed inside the placement rack in the existing cell suspension preparation device, due to different amounts of the original cell solution required according to experimental needs, test tubes of different specifications and sizes will be used to hold the original cell solution. Since the placement rack of a general centrifuge is directly fixed inside the centrifuge, it is difficult to randomly replace the corresponding placement rack according to the different specifications and sizes of the test tubes. When centrifuging a large number of original cell solutions, it is necessary to load them into test tubes in batches for centrifugation operations, which will require multiple centrifugation operations, affecting the preparation efficiency of the cell suspension. At the same time, the cover of the centrifuge is generally opened or closed by manual flipping. Since the cover is heavy, a large amount of force is required to flip it open, and the operation may be laborious. Especially for application scenarios that require frequent opening and closing, it may affect the working efficiency.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] A device for preparing cell suspension, including a centrifuge housing. At the top of both sides of the centrifuge housing, a pushing structure is symmetrically provided. At the bottom of the centrifuge housing, a cross plate is provided. At the central position of the top of the centrifuge housing, a rotating structure is rotatably installed. On the surface of the rotating structure at the top inside the centrifuge housing, a placement rack is provided, and the placement rack is rotatably installed on the top of the centrifuge housing. At the top end of the rotating structure, a pressing cover is provided, and the pressing cover covers the top of the placement rack. Inside the placement rack, there are sixteen capped test tubes. At the bottom of both sides of the centrifuge housing, support frames are respectively rotatably installed.

[0008] As a further solution of the present invention, on one side of the top of the centrifuge housing, a centrifuge cover is rotatably installed. On both sides of the centrifuge cover, connecting convex blocks are respectively fixedly installed. At the bottom of both sides of the centrifuge housing, rectangular grooves are respectively provided. At the central position of the bottom of the centrifuge housing, an installation groove is provided. At the bottom of the installation groove, four groups of fixed card slots are equiangularly provided. On the top of the centrifuge housing, a placement groove is provided. On the inner wall of the placement groove, two groups of annular chutes are provided.

[0009] As a further solution of the present invention, the pushing structure includes a T-shaped slide bar. On the surface of the T-shaped slide bar, a moving slider is slidably installed. On the top of the moving slider, a pushing bar is rotatably installed, and the end of the pushing bar away from the moving slider is rotatably connected to the connecting convex block. At the bottom of one side of the moving slider, an electric push rod is fixedly installed, and the electric push rod is fixedly connected to the surface of the centrifuge housing.

[0010] As a further solution of the present invention, fixing bolts are provided at the four corners of the cross plate, and the positions of the fixing bolts correspond to those of the fixing card slots. A rotating motor is fixedly installed at the top of the cross plate, and the output end of the rotating motor is fixedly installed with a driving gear, and the driving gear is located at the inner top of the installation slot.

[0011] As a further solution of the present invention, the rotating structure includes a driven gear. The driven gear is rotatably connected to the inside of the installation slot, and the driven gear meshes with the driving gear. The top of the driven gear passes through the installation slot and extends into the placement slot to be fixedly installed with a first connecting column. Six groups of first limiting sliding strips are fixedly installed on the surface of the first connecting column at equal angles. A second connecting column is fixedly installed at the top of the first connecting column. Three groups of second limiting sliding strips are fixedly installed on the surface of the second connecting column at equal angles. A fixing stud is fixedly installed at the top end of the second limiting sliding strip, and a fixing nut sleeve is provided on the surface of the fixing stud.

[0012] As a further solution of the present invention, a plurality of sliding balls are embedded and installed on the surface of the placement rack at equal angles. The positions of the sliding balls correspond to those of the annular sliding grooves, and the sliding balls are rotatably connected to the inside of the annular sliding grooves. Sixteen test tube slots are provided in the placement rack at equal angles, and two test tube slots are in a group. A rubber clamping ring is fixedly installed on the inner wall of the test tube slot. A first installation through hole is provided at the inner bottom of the placement rack, and the first installation through hole penetrates the placement rack. Six groups of first limiting card slots are provided on the inner wall of the first installation through hole at equal angles, and the positions of the first limiting card slots correspond to those of the first limiting sliding strips.

[0013] As a further solution of the present invention, eight fixing pressure grooves are provided on the surface of the pressing cover at equal angles, and the positions of the fixing pressure grooves correspond to those of each group of test tube slots. A second installation through hole is provided at the central position of the pressing cover, and the second installation through hole penetrates the pressing cover. Three groups of second limiting card slots are provided on the inner wall of the second installation through hole at equal angles, and the positions of the second limiting card slots correspond to those of the second limiting sliding strips.

[0014] As a further solution of the present invention, rubber cushion pads are fixedly installed on both sides of the support frame. Electric push frames are symmetrically embedded and fixedly installed on one side of the support frame. One end of the two electric push frames extends to the outside of the support frame and is rotatably installed with the same rotating frame. A rubber anti-slip pad is fixedly installed at the bottom of the rotating frame.

[0015] Compared with the prior art, the beneficial effects of the present invention are:

[0016] 1. The present invention is provided with a centrifuge housing, a first connecting column, a second connecting column, a placement rack and a pressing cover. After lifting the centrifuge cover plate on the top of the centrifuge housing, turn the fixing sleeve off the fixing stud, place the placement rack on the surface of the first connecting column, and make the placement rack correspondingly fit on the surface of the first limiting card strip until the placement rack is inserted into the placement groove. Make the sliding balls on the surface of the placement rack fit into the corresponding annular sliding grooves. After inserting the capped test tubes into the test tube grooves in pairs, then place the pressing cover on the surface of the second connecting column, and make the pressing cover tightly adhere to the top of the placement rack, ensuring that the fixing pressure groove on the surface of the pressing cover is stuck on the top of the capped test tube. At the same time, the second limiting card slot inside the pressing cover is stuck on the surface of the corresponding second limiting slide bar. Tighten the fixing sleeve to tightly adhere to the top of the pressing cover and fix the pressing cover on the top of the placement rack, thus facilitating the disassembly and assembly of placement racks, capped test tubes and pressing covers of different specifications according to the needs of cell suspension preparation.

[0017] 2. The present invention is provided with a centrifuge cover plate, a connecting convex block, a T-shaped slide bar, a moving slider, a pushing bar and an electric push rod. Control the two electric push rods to extend synchronously, drive the moving slider at the output end of the electric push rod to slide on the surface of the T-shaped slide bar, and make the pushing bar rotate on the top of the moving slider. At the same time, one end of the pushing bar far from the moving slider rotates on the surface of the connecting convex block, jack up the connecting convex block and drive the centrifuge cover plate to rotate on the top of the centrifuge housing to lift the top of the centrifuge housing, which is convenient for correspondingly installing the placement rack, capped test tube and pressing cover into the placement groove on the top of the centrifuge housing. After the placement rack, capped test tube and pressing cover are installed in the appropriate positions, control the two electric push rods to contract synchronously, drive the moving slider to slide reversely on the surface of the T-shaped slide bar, and make the pushing bar rotate on the surfaces of the moving slider and the connecting convex block, pull the centrifuge cover plate to flip and close on the top of the centrifuge housing to ensure that the capped test tube will not fly out of the centrifuge housing when rotating at high speed inside the centrifuge housing.

[0018] 3. The present invention is provided with a support frame, an electric push frame, a rotating frame and a rubber anti-slip pad. After placing the centrifuge housing on a horizontal tabletop, then turn the support frame out of the rectangular groove, control the two electric push frames to expand and contract synchronously, make the rotating frame push out the support frame until the rubber anti-slip pad at the bottom of the rotating frame tightly adheres to the tabletop. Repeat the steps so that the rubber anti-slip pads at the bottoms of the two rotating frames tightly adhere to the tabletop, make the support frame and the electric push frame tilt on both sides of the centrifuge housing. At the same time, the rotating frame applies a downward pressure on the rubber anti-slip pad, squeezing the rubber anti-slip pad to deform on the tabletop, increasing the friction of the rubber anti-slip pad on the tabletop, so that the support frame and the electric push frame support the centrifuge housing, avoiding the centrifuge housing from sliding and toppling on the tabletop during high-speed centrifugal rotation. Description of the Drawings

[0019] To more clearly illustrate the technical solutions of the embodiments of the present invention, the following will briefly introduce the drawings required for the description of the embodiments. Obviously, the drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can be obtained based on these drawings.

[0020] Figure 1 is the developed sectional structure schematic diagram of the present invention;

[0021] Figure 2 is the developed structure schematic diagram of the centrifuge housing and the centrifuge cover plate of the present invention;

[0022] Figure 3 is the developed sectional structure schematic diagram of the centrifuge housing and the rotating structure of the present invention;

[0023] Figure 4 is the structure schematic diagram of the T-shaped slide bar, the moving slider and the electric push rod of the present invention;

[0024] Figure 5 is the structure schematic diagram of the driven gear, the first connecting column and the second connecting column of the present invention;

[0025] Figure 6 is the sectional structure schematic diagram of the placement rack and the test tube with a cover of the present invention;

[0026] Figure 7 is the sectional structure schematic diagram of the pressing cover and the second limit card slot of the present invention;

[0027] Figure 8 is the structure schematic diagram of the cross plate, the rotating motor and the driving gear of the present invention;

[0028] Figure 9 is the sectional structure schematic diagram of the support frame, the electric push frame and the rotating frame of the present invention.

[0029] In the drawings, the list of components represented by each reference numeral is as follows:

[0030] 1. Centrifuge housing; 101. Centrifuge cover plate; 102. Connecting bump; 103. Rectangular groove; 104. Mounting groove; 105. Fixed card slot; 106. Placing groove; 107. Annular sliding groove; 2. Pushing structure; 201. T-shaped slide bar; 202. Moving slider; 203. Pushing bar; 204. Electric push rod; 3. Cross plate; 301. Fixed bolt; 302. Rotating motor; 303. Driving gear; 4. Rotating structure; 401. Driven gear; 402. First connecting column; 403. First limiting slide bar; 404. Second connecting column; 405. Second limiting slide bar; 406. Fixed stud; 407. Fixed nut sleeve; 5. Placing rack; 501. Sliding ball; 502. Test tube slot; 503. Rubber clamping ring; 504. First mounting through hole; 505. First limiting card slot; 6. Test tube with lid; 7. Pressing cover; 701. Fixed pressing groove; 702. Second mounting through hole; 703. Second limiting card slot; 8. Support frame; 801. Rubber cushion; 802. Electric push frame; 803. Rotating frame; 804. Rubber anti-slip pad. Detailed implementation manners

[0031] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0032] Please refer to Figures 1-9 , the present invention provides a technical solution:

[0033] A cell suspension preparation device includes a centrifuge housing 1. Pushing structures 2 are symmetrically arranged at the top of both sides of the centrifuge housing 1. A cross plate 3 is arranged at the bottom of the centrifuge housing 1. A rotating structure 4 is rotatably installed at the central position of the top of the centrifuge housing 1. A placing rack 5 is arranged on the surface of the top of the rotating structure 4 inside the centrifuge housing 1, and the placing rack 5 is rotatably installed on the top of the centrifuge housing 1. A pressing cover 7 is arranged at the top of the rotating structure 4, and the pressing cover 7 covers the top of the placing rack 5. Sixteen test tubes with lids 6 are arranged inside the placing rack 5. Support frames 8 are respectively rotatably installed at the bottom of both sides of the centrifuge housing 1;

[0034] During operation, after fixing the cross plate 3 at the bottom of the centrifuge housing 1, move the centrifuge housing 1 flat on the table, unfold the support frames 8 on both sides of the centrifuge housing 1 to support the centrifuge housing 1, then insert multiple capped test tubes 6 pairwise into the inner wall of the placement rack 5 until the inner wall of the placement rack 5 is filled with an appropriate number of capped test tubes 6. Move the placement rack 5 and suspend it on the top of the centrifuge housing 1 so that the placement rack 5 corresponds to the position of the rotating structure 4. Move the placement rack 5 close to the rotating structure 4 until the placement rack 5 is sleeved on the surface of the rotating structure 4, and at the same time, make the placement rack 5 snap into the top of the centrifuge housing 1. Then, suspend the pressing cover 7 correspondingly on the top of the placement rack 5, move the pressing cover 7 and press it tightly and fixedly on the top of the placement rack 5 so that the pressing cover 7 clamps and fixes the capped test tubes 6 on the inner wall of the placement rack 5. Control the moving and telescoping of the pushing structure 2 to close the centrifuge housing 1, and make the rotating structure 4 drive the placement rack 5 and the capped test tubes 6 to rotate highly inside the centrifuge housing 1 until the cell stock solution inside the capped test tubes 6 is gradually stratified;

[0035] As a further solution of the present invention, one side of the top of the centrifuge housing 1 is rotatably installed with a centrifuge cover plate 101. Connecting convex blocks 102 are respectively fixed on both sides of the centrifuge cover plate 101. Rectangular grooves 103 are respectively provided at the bottoms of both sides of the centrifuge housing 1. An installation groove 104 is provided at the central position of the bottom of the centrifuge housing 1. Four groups of fixed card slots 105 are equiangularly provided at the bottom of the installation groove 104. A placement groove 106 is provided at the top of the centrifuge housing 1. Two groups of annular sliding grooves 107 are provided on the inner wall of the placement groove 106;

[0036] During operation, control the centrifuge cover plate 101 to rotate on the top of the centrifuge housing 1 so that the centrifuge cover plate 101 can cover the top of the centrifuge housing 1 to shield and protect the placement rack 5 and the pressing cover 7 rotating inside the placement groove 106, and prevent the capped test tubes 6 inside the placement rack 5 from detaching from the placement rack 5 due to the too high rotation speed of the placement rack 5.

[0037] As a further solution of the present invention, the pushing structure 2 includes a T-shaped slide bar 201. A moving slider 202 is slidably installed on the surface of the T-shaped slide bar 201. A pushing bar 203 is rotatably installed on the top of the moving slider 202, and one end of the pushing bar 203 away from the moving slider 202 is rotatably connected to the connecting convex block 102. An electric push rod 204 is fixedly installed at the bottom of one side of the moving slider 202, and the electric push rod 204 is fixedly connected to the surface of the centrifuge housing 1;

[0038] During operation, the electric push rod 204 is controlled to extend and retract to push the moving slider 202 to slide on the surface of the T-shaped slide bar 201, causing the push bar 203 to rotate on the top of the moving slider 202. At the same time, one end of the push bar 203 away from the moving slider 202 rotates on the surface of the connecting bump 102, thereby controlling the push bar 203 to lift the push connecting bump 102, enabling the centrifuge cover plate 101 to flip open or close on the top of the centrifuge housing 1. Meanwhile, when the moving slider 202 slides on the surface of the T-shaped slide bar 201, the T-shaped slide bar 201 limits the moving slider 202 to ensure that the moving slider 202 does not tilt or deflect during the sliding process.

[0039] As a further solution of the present invention, fixing bolts 301 are provided at the four corners of the cross plate 3, and the positions of the fixing bolts 301 correspond to those of the fixing card slots 105. A rotating motor 302 is fixedly installed on the top of the cross plate 3, and an output end of the rotating motor 302 is fixedly installed with a driving gear 303, and the driving gear 303 is located at the inner top of the installation slot 104.

[0040] During operation, the cross plate 3 is clamped at the bottom of the installation slot 104, so that the four corners of the cross plate 3 are inserted into the corresponding fixing card slots 105. The fixing bolts 301 are tightened to clamp and fix the cross plate 3, so that the rotating motor 302 and the driving gear 303 are inserted into the installation slot 104. At the same time, the driving gear 303 meshes with the driven gear 401. Since the diameter of the driving gear 303 is larger than that of the driven gear 401, the rotation speed of the driven gear 401 is increased, enabling the first connecting column 402 to drive the placement rack 5 to rotate at a high speed.

[0041] As a further solution of the present invention, the rotating structure 4 includes a driven gear 401. The driven gear 401 is rotatably connected inside the installation slot 104 and meshes with the driving gear 303. The top of the driven gear 401 extends through the installation slot 104 into the placement slot 106 and is fixedly installed with a first connecting column 402. Six groups of first limiting slide bars 403 are fixedly installed on the surface of the first connecting column 402 at equal angles. A second connecting column 404 is fixedly installed on the top of the first connecting column 402. Three groups of second limiting slide bars 405 are fixedly installed on the surface of the second connecting column 404 at equal angles. A fixing stud 406 is fixedly installed at the top end of the second limiting slide bar 405, and a fixing nut 407 is provided on the surface of the fixing stud 406.

[0042] During operation, the placement rack 5 is sleeved on the surface of the first connecting column 402, so that the first limit card slot 505 is stuck on the surface of the corresponding first limit slide bar 403. Then, after inserting the capped test tube 6 into the placement rack 5, the pressing cover 7 is pressed against the top of the placement rack 5, ensuring that the second limit card slot 703 on the inner wall of the pressing cover 7 is stuck on the surface of the corresponding second limit slide bar 405. Then, the fixing nut 407 is tightened to press and fix the pressing cover 7 on the top of the placement rack 5. The rotation motor 302 is started to drive the driving gear 303 to rotate. Through the meshing transmission of the driving gear 303 and the driven gear 401, the first connecting column 402 and the second connecting column 404 are driven to rotate, so that the placement rack 5 and the pressing cover 7 rotate at high speed synchronously inside the placement groove 106, and the cell stock solution inside the capped test tube 6 is centrifuged by rotation. At the same time, the placement rack 5 and the pressing cover 7 are stuck on the surfaces of the corresponding first connecting column 402 and the second connecting column 404, and the first limit slide bar 403 and the second limit slide bar 405 limit the placement rack 5 and the pressing cover 7, ensuring that the placement rack 5 and the pressing cover 7 can rotate synchronously with the first connecting column 402 and the second connecting column 404 without relative sliding deflection.

[0043] As a further solution of the present invention, a plurality of sliding balls 501 are embedded and installed on the surface of the placement rack 5 at equal angles. The positions of the sliding balls 501 correspond to those of the annular chute 107, and the sliding balls 501 are in rolling connection with the inside of the annular chute 107. Sixteen test tube slots 502 are provided at equal angles inside the placement rack 5, and two test tube slots 502 form a group. A rubber clamping ring 503 is fixedly installed on the inner wall of the test tube slot 502. A first installation through hole 504 is provided at the inner bottom of the placement rack 5, and the first installation through hole 504 penetrates through the placement rack 5. Six groups of first limit card slots 505 are provided at equal angles on the inner wall of the first installation through hole 504, and the positions of the first limit card slots 505 correspond to those of the first limit slide bars 403;

[0044] During operation, after inserting the placement rack 5 into the placement groove 106, the capped test tubes 6 are inserted into the corresponding test tube slots 502 in pairs. When the capped test tubes 6 are inserted into the test tube slots 502, the capped test tubes 6 squeeze the rubber clamping rings 503 on the inner walls of the test tube slots 502 to deform, so that the rubber clamping rings 503 are sleeved on the surfaces of the capped test tubes 6, and the capped test tubes 6 are clamped and fixed inside the test tube slots 502. At the same time, the placement rack 5 is stuck inside the placement groove 106, and the sliding balls 501 are pressed against the inside of the annular chute 107, so that when the placement rack 5 rotates inside the placement groove 106, the sliding balls 501 roll inside the corresponding annular chute 107, thereby reducing the friction when the outer surface of the placement rack 5 rotates on the inner wall of the placement groove 106.

[0045] As a further solution of the present invention, eight fixed pressing grooves 701 are provided on the surface of the pressing cover 7 at equal angles, and the fixed pressing grooves 701 correspond to the positions of each group of test tube grooves 502. A second installation through hole 702 is provided at the central position of the pressing cover 7, and the second installation through hole 702 penetrates through the pressing cover 7. Three groups of second limit card slots 703 are provided on the inner wall of the second installation through hole 702 at equal angles, and the second limit card slots 703 correspond to the positions of the second limit sliding strips 405.

[0046] During operation, when the pressing cover 7 is covered on the top of the placement rack 5, the fixed pressing grooves 701 are stuck on the surface of the covered test tube 6 corresponding inside the placement rack 5, so as to press and fix the covered test tube 6 inside the placement rack 5, ensuring that the covered test tube 6 will not break away when rotating at high speed with the placement rack 5. At the same time, the second installation through hole 702 is sleeved on the surface of the second connecting column 404, so that the second limit card slots 703 are stuck on the surface of the corresponding second limit sliding strips 405, ensuring that the pressing cover 7 can rotate synchronously with the second connecting column 404.

[0047] As a further solution of the present invention, rubber cushion pads 801 are fixedly installed on both sides of the support frame 8 respectively. Electric push frames 802 are symmetrically and fixedly installed on one side of the support frame 8. One ends of the two electric push frames 802 extend to the outside of the support frame 8 and are rotatably installed with the same rotating frame 803. A rubber anti-slip pad 804 is fixedly installed at the bottom of the rotating frame 803.

[0048] During operation, when the support frame 8 is stuck inside the rectangular groove 103, the rubber anti-slip pad 804 is deformed by extrusion between the support frame 8 and the inner wall of the rectangular groove 103, so as to clamp and fix the support frame 8 inside the rectangular groove 103. When the centrifuge housing 1 is placed on a horizontal tabletop, the support frame 8 is rotated out of the rectangular groove 103, and the electric push frame 802 is controlled to extend, so that the rubber anti-slip pad 804 at the bottom of the rotating frame 803 is closely attached to the tabletop, and the rubber anti-slip pad 804 is deformed on the tabletop, increasing the friction of the rubber anti-slip pad 804 on the tabletop. At the same time, the support frame 8 and the electric push frame 802 are inclined on both sides of the centrifuge housing 1 to support the centrifuge housing 1, preventing the centrifuge housing 1 from sliding randomly on the tabletop during high-speed centrifugal rotation.

[0049] The working principle of the present invention:

[0050] Insert the rotating motor 302 and the driving gear 303 into the inside of the installation groove 104, so that the driving gear 303 meshes with the driven gear 401. At the same time, the cross plate 3 is stuck inside the corresponding fixed clamping groove 105, and the fixing bolt 301 is tightened to fix the cross plate 3, thereby fixing the rotating motor 302 and the driving gear 303 inside the installation groove 104. Then, place the centrifuge housing 1 on the horizontal tabletop, rotate out the support frame 8 from inside the rectangular groove 103, control the electric push frames 802 on both sides of the support frame 8 to push the rotating frame 803 to move away from the support frame 8, rotate the rotating frame 803 at one end of the electric push frame 802 to be parallel to the tabletop, and make the rubber anti-slip pad 804 tightly adhere to the top of the tabletop. Under the thrust of the electric push frame 802, the rubber anti-slip pad 804 is squeezed and deformed, thereby increasing the friction of the rubber anti-slip pad 804 on the tabletop;

[0051] Control the two groups of electric push rods 204 to extend synchronously to push the moving slider 202 to slide on the surface of the corresponding T-shaped slide bar 201, so that the push bar 203 rotates between the moving slider 202 and the connecting lug 102. When the push bar 203 moves with the moving slider 202, the push bar 203 jacks up the connecting lug 102, so that the centrifuge cover 101 is flipped and opened from the top of the centrifuge housing 1, and the placement groove 106 is exposed. Unscrew the fixed sleeve 407 from the surface of the fixed stud 406, and suspend the placement rack 5 on the top of the placement groove 106. After the first limit clamping groove 505 on the inner wall of the first installation through hole 504 inside the placement rack 5 corresponds to the position of the first limit slide bar 403 on the surface of the first connecting column 402, the placement rack 5 is sleeved on the surface of the first connecting column 402, so that the first limit clamping groove 505 is stuck on the surface of the corresponding first limit slide bar 403. At the same time, the placement rack 5 is inserted into the placement groove 106, and the sliding balls 501 on the surface of the placement rack 5 are tightly adhered to the corresponding annular sliding groove 107 inside the inner wall of the placement groove 106;

[0052] Take an appropriate amount of capped test tubes 6. After injecting an appropriate amount of cell stock solution into the interior of the capped test tubes 6, insert the capped test tubes 6 pairwise into the test tube slots 502 inside the placement rack 5 such that the rubber retaining rings 503 inside the test tube slots 502 are squeezed and deformed to tightly clamp and fix the capped test tubes 6 inside the test tube slots 502. Move the pressing cover 7 and suspend it on top of the placement rack 5 so that the fixed pressing grooves 701 on the surface of the pressing cover 7 correspond to the positions of the capped test tubes 6 inside the placement rack 5. Move the pressing cover 7 to closely adhere to the top of the placement rack 5 so that the fixed pressing grooves 701 cover the surfaces of the capped test tubes 6, tightly clamp and fix the capped test tubes 6 inside the test tube slots 502. At the same time, the second installation through holes 702 inside the pressing cover 7 cover the surfaces of the second connecting columns 404 such that the second limiting card slots 703 are stuck on the surfaces of the corresponding second limiting sliding bars 405. Tighten the fixed screw sleeve 407 to closely adhere to the top of the pressing cover 7, fix the pressing cover 7 on top of the placement rack 5, such that the pressing cover 7 inserts and fixes the capped test tubes 6 into the corresponding test tube slots 502. At the same time, make the placement rack 5 tightly clamped inside the placement groove 106;

[0053] Control the electric push rod 204 to contract, drive the moving slider 202 to slide on the T-shaped sliding bar 201, such that the moving slider 202 drives the pushing bar 203 to move. At the same time, the pushing bar 203 pulls the connecting lug 102 to cover the centrifuge cover plate 101 on top of the centrifuge housing 1, providing shielding protection for the placement rack 5 and the pressing cover 7 inside the centrifuge housing 1. Start the rotating motor 302 to control the driving gear 303 to rotate. Under the meshing drive of the driving gear 303 and the driven gear 401, control the driven gear 401 to rotate at high speed inside the installation groove 104, drive the first connecting column 402 and the second connecting column 404 on top of the driven gear 401 to rotate synchronously. Under the clamping and limiting action of the first limiting sliding bar 403 and the second limiting sliding bar 405, drive the placement rack 5 and the pressing cover 7 to rotate synchronously at high speed inside the placement groove 106, such that the capped test tubes 6 clamped inside the test tube slots 502 rotate at high speed until the cell stock solution inside the capped test tubes 6 is centrifuged and stratified to make a cell suspension. After centrifugal rotation for a certain period of time, stop the rotating motor 302, such that the placement rack 5 and the pressing cover 7 are stationary inside the placement groove 106. Control the centrifuge cover plate 101 to be lifted, loosen the fixed screw sleeve 407 to remove the pressing cover 7 from the top of the placement rack 5, and thus extract the capped test tubes 6 inserted inside the test tube slots 502.

Claims

1. A cell suspension preparation device, comprising a centrifuge housing (1), characterized in that: The tops of both sides of the centrifuge housing (1) are symmetrically provided with push structures (2); the bottom of the centrifuge housing (1) is provided with a cross plate (3); a rotating structure (4) is rotatably mounted at the central position of the top of the centrifuge housing (1); a placement rack (5) is provided on the top of the rotating structure (4) located inside the centrifuge housing (1); and the placement rack (5) is rotatably mounted on the top of the centrifuge housing (1); a pressing cover (7) is provided on the top of the rotating structure (4); and the pressing cover (7) covers the top of the placement rack (5); sixteen test tubes (6) with covers are arranged inside the placement rack (5); and support racks (8) are rotatably mounted on the bottoms of both sides of the centrifuge housing (1).

2. A cell suspension preparation device according to claim 1, characterized in that: A centrifuge cover plate (101) is rotatably mounted on one side of the top of the centrifuge housing (1), connecting protrusions (102) are fixedly mounted on both sides of the centrifuge cover plate (101), rectangular grooves (103) are respectively arranged at the bottom of both sides of the centrifuge housing (1), a mounting groove (104) is arranged at the central position of the bottom of the centrifuge housing (1), four groups of fixed slots (105) are arranged at equal angles at the bottom of the mounting groove (104), a placement groove (106) is arranged at the top of the centrifuge housing (1), and two groups of annular sliding grooves (107) are arranged on the inner wall of the placement groove (106).

3. A cell suspension preparation device according to claim 2, characterized in that: The pushing structure (2) comprises a T-shaped slide bar (201), a movable slider (202) is slidably mounted on the surface of the T-shaped slide bar (201), a pushing bar (203) is rotatably mounted on the top of the movable slider (202), and one end of the pushing bar (203) away from the movable slider (202) is rotatably connected to the connecting protrusion (102), an electric push rod (204) is fixedly mounted on the bottom of one side of the movable slider (202), and the electric push rod (204) is fixedly connected to the surface of the centrifuge housing (1).

4. A cell suspension preparation device according to claim 2, characterized in that: The four corners of the cross plate (3) are provided with fixing bolts (301), and the positions of the fixing bolts (301) correspond to the positions of the fixing slots (105). A rotating motor (302) is fixedly mounted on the top of the cross plate (3), and a driving gear (303) is fixedly mounted on the output end of the rotating motor (302), and the driving gear (303) is located at the top of the mounting slot (104).

5. A cell suspension preparation device according to claim 4, characterized in that: The rotating structure (4) comprises a driven gear (401), the driven gear (401) is rotatably connected to the inside of the installation slot (104), and the driven gear (401) and the driving gear (303) are meshed with each other, the top of the driven gear (401) passes through the installation slot (104) and extends to the inside of the placement slot (106), and a first connecting column (402) is fixedly installed, six groups of first limiting slide bars (403) are fixedly installed at equal angles on the surface of the first connecting column (402), a second connecting column (404) is fixedly installed on the top of the first connecting column (402), and three groups of second limiting slide bars (405) are fixedly installed at equal angles on the surface of the second connecting column (404), and a fixing stud (406) is fixedly installed on the top of the second limiting slide bar (405), and a fixing screw sleeve (407) is provided on the surface of the fixing stud (406).

6. A cell suspension preparation device according to claim 5, characterized in that: The surface of the placement rack (5) is inlaid with multiple groups of sliding balls (501) in rolling manner at equal angles, the sliding balls (501) correspond to the positions of the annular slide groove (107), and the sliding balls (501) are rollingly connected to the inside of the annular slide groove (107), sixteen test tube grooves (502) are arranged at equal angles inside the placement rack (5), and two test tube grooves (502) form a group, and a rubber clamping ring (503) is fixedly installed on the inner wall of the test tube groove (502), a first mounting through hole (504) is arranged at the bottom of the placement rack (5), and the first mounting through hole (504) passes through the placement rack (5), and six groups of first limit slots (505) are arranged on the inner wall of the first mounting through hole (504) at equal angles, and the first limit slots (505) correspond to the positions of the first limit slide bar (403).

7. A cell suspension preparation device according to claim 6, characterized in that: The surface of the clamping cover (7) is provided with eight fixed pressing grooves (701) at equal angles, and the fixed pressing grooves (701) correspond to the position of each group of test tube grooves (502); a second mounting through hole (702) is provided at the central position of the clamping cover (7), and the second mounting through hole (702) passes through the clamping cover (7); three groups of second limiting grooves (703) are provided at equal angles on the inner wall of the second mounting through hole (702), and the second limiting grooves (703) correspond to the position of the second limiting slide bar (405).

8. A cell suspension preparation device according to claim 1, characterized in that: Rubber pads (801) are fixedly installed on both sides of the support frame (8), and an electric push frame (802) is symmetrically inlaid and fixedly installed on one side of the support frame (8). One end of the two groups of electric push frames (802) extends to the outside of the support frame (8) and is rotatably installed with the same rotating frame (803), and a rubber anti-skid pad (804) is fixedly installed on the bottom of the rotating frame (803).

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