Cultivation method using toadstool undisturbed soil

Abstract

The invention discloses a cultivation method using toadstool undisturbed soil, and belongs to the technical field of toadstool planting, the toadstool undisturbed soil is dug in a wild toadstool fruiting field, a toadstool bed is dug in a cultivation field, the toadstool undisturbed soil is transferred to the dug toadstool bed, the toadstool undisturbed soil is spread flat, then a composite film forming solution is sprayed, and the toadstool undisturbed soil is compacted; the method comprises the following steps: laying undisturbed soil of morchella esculenta, placing nutrition bags filled with morchella esculenta grains on the undisturbed soil of the morchella esculenta, in the cultivation process, building an arched shed, controlling the temperature to be 16-18 DEG C and the humidity to be 60-75%, planting the morchella esculenta under the luminous flux of 500-700 lumens, ventilating once every two days, and harvesting when the diameter of the pileus of the morchella esculenta is 5-7cm. According to the method, microbial communities and nutritional ingredients in the toadstool undisturbed soil are reserved, and the toadstool undisturbed soil can be used for growth and reproduction of wild toadstools, so that the cultivation efficiency of the wild toadstools is improved, and wild toadstool resources are protected.

Description

Technical Field

[0001] The invention belongs to the technical field of morel cultivation, and in particular relates to a cultivation method using original soil of morels. Background Art

[0002] Morchella is a precious edible mushroom, with a cap that is nearly spherical, ovate or elliptical, with a blunt top and pits on the surface that resemble a tripe. Morchella grows mostly on the humus layer of broad-leaved forests or mixed coniferous and broad-leaved forests, mainly in humus-rich sandy loam or brown soil, brown soil, etc. It is more likely to occur on forest land after fire. In addition, Morchella likes moisture, and the growth environment must maintain humidity. In early spring, the temperature of 4-16℃ within a few weeks can stimulate the formation of Morchella fruiting bodies.

[0003] Morchella is rich in nutritional value, containing vitamins, minerals, amino acids, proteins, carbohydrates and other nutrients, which help to supplement the body's nutrients and energy, increase appetite, improve immunity and promote metabolism. Modern research has also found that Morchella contains polysaccharides, which have anti-cancer and anti-tumor effects.

[0004] The cultivation of morels requires certain techniques and conditions. During the cultivation process, attention should be paid to the management of humidity, temperature, pest control, etc. However, the existing cultivation methods generally require re-soil preparation, soil pH adjustment, artificial inoculation, etc., which is not conducive to improving the cultivation efficiency of morels. Summary of the invention

[0005] The purpose of the present invention is to provide a cultivation method using authentic morel soil, which makes full use of wild resources, retains the microbial community and nutrients in the authentic morel soil, can be used for the growth and reproduction of wild morels, thereby improving the cultivation efficiency of wild morels and protecting wild morel resources.

[0006] The purpose of the present invention can be achieved through the following technical solutions:

[0007] A cultivation method using undisturbed soil of Morchella comprises the following steps:

[0008] Step 1: In the wild morel mushroom field, dig 10-20cm thick original soil from the surface of the morel as cultivation soil; dig a mushroom bed in the cultivation field, transfer the collected original soil from the wild morel mushroom field to the dug mushroom bed, and spread it flat according to 1-1.5kg / m 2 Spray the composite film-forming liquid in appropriate amount and compact it.

[0009] Step 2: Place nutrient bags on the prepared original soil for morels, with a dosage of 1500-2000kg / mu. The nutrient bags are filled with morel fungus grains, which are obtained by mixing rice bran and wheat in a mass ratio of 1:3 and then sterilized at high temperature, and have a water content of 45%.

[0010] Step 3: During the cultivation process, an arch shed is built, the temperature is controlled at 16-18°C and the humidity is controlled at 60-75%, and the morels are planted under the condition of a light flux of 500-700 lumens. Ventilation is performed every 2 days to avoid mold growth. The morels can be harvested when the cap diameter is 5-7cm, thereby completing the cultivation method using the original soil of the morels.

[0011] Furthermore, the composite film-forming solution is prepared by the following steps:

[0012] Step 1: Dissolve polyethylene glycol in dichloromethane and transfer it to a reactor, then drop a 15% mass fraction of p-toluenesulfonic acid chloride triethylamine solution into the reactor, keep the reaction at 20-25°C for 10-12 hours, wash the reaction solution 2-3 times with a hydrochloric acid solution with a molar concentration of 3 mol / L, combine the organic phases and adjust the pH value to 7 with sodium bicarbonate, filter, and concentrate the filtrate under reduced pressure to obtain esterified polyethylene glycol.

[0013] Step 2: Add esterified polyethylene glycol and 28% ammonia water into a reactor, keep the reaction at 135-140°C for 5-6 hours, extract the reaction product with DCM three times, combine the organic phases, adjust the pH value to 11.5-12 with a sodium hydroxide solution with a molar concentration of 1 mol / L, stir for 4-5 hours at 20-25°C and 200-300 r / min, let stand, separate the organic phase and wash with a saturated sodium chloride solution until neutral, filter, dry the filtrate with anhydrous sodium sulfate to remove water, let stand, filter, and concentrate under reduced pressure to obtain amino-terminated polyethylene glycol.

[0014] Step 3: Add amino-terminated polyethylene glycol, lignin nanoparticles, potassium humate and deionized water into a reactor, stir for 30-40 minutes at 70-80 and 200-300 r / min, then add dialdehyde starch, anhydrous methanol, boric acid, glutaraldehyde and 10% acetic acid solution into the reactor, continue stirring for 20-30 minutes to obtain a composite film-forming solution.

[0015] Furthermore, in step 1, the mass ratio of polyethylene glycol, dichloromethane and triethylamine solution of p-toluenesulfonic acid chloride is 2:10:5-6.

[0016] Furthermore, in step 2, the mass ratio of esterified polyethylene glycol to aqueous ammonia is 1:4.

[0017] Furthermore, in step 3, the mass ratio of terminal amino polyethylene glycol, lignin nanoparticles, potassium humate, deionized water, dialdehyde starch, anhydrous methanol, boric acid, glutaraldehyde and acetic acid solution is 1:2:10:250:20:30:10:10:20.

[0018] Further, lignin nanoparticles are prepared by the following steps:

[0019] Ammonium lignin sulfonate and deionized water are added into a reactor and stirred to dissolve. Anhydrous ethanol is added dropwise into the reactor under stirring conditions of 200-300 r / min to induce the ammonium lignin sulfonate to assemble into lignin nanoparticles with uniform particle size. After the addition is completed, the anhydrous ethanol is removed by rotary evaporation and vacuum drying is performed to obtain lignin nanoparticles.

[0020] Furthermore, the usage ratio of ammonium lignin sulfonate, deionized water and anhydrous ethanol is 0.1 g: 15 mL: 25-30 mL.

[0021] Beneficial effects of the present invention:

[0022] The present invention utilizes the cultivation method of the original soil of morels, fully utilizes wild resources, retains the microbial community and nutrients in the original soil of morels, can be used for the growth and reproduction of wild morels, thereby improving the cultivation efficiency of wild morels and protecting wild morel resources.

[0023] The aldehyde groups in the dialdehyde starch and glutaraldehyde in the composite film-forming liquid of the present invention can be cross-linked with the amino groups in the amino-terminated polyethylene glycol, and the boric acid can be cross-linked with raw materials such as lignin nanoparticles, which is conducive to the formation of a three-dimensional network and improves the stability of the cross-linked gel film. The original soil of Morchella is treated with the composite film-forming liquid, which helps to increase the hard substances such as organic matter in the cultivation soil, improve the soil structure, improve its water and fertilizer retention capacity, and help the growth and reproduction of wild Morchella. DETAILED DESCRIPTION

[0024] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0025] Embodiment 1: This embodiment provides a cultivation method using undisturbed soil of Morchella, comprising the following steps:

[0026] S1: 1 kg of ammonium lignin sulfonate and 150 L of deionized water were added to a reactor and stirred to dissolve. 300 L of anhydrous ethanol was added dropwise to the reactor under stirring at 300 r / min to induce the ammonium lignin sulfonate to assemble into lignin nanoparticles with uniform particle size. After the addition was completed, the anhydrous ethanol was removed by rotary evaporation and vacuum dried to obtain lignin nanoparticles.

[0027] S2: Dissolve 2 kg of polyethylene glycol in 10 kg of dichloromethane and transfer to a reactor. Then, drop 6 kg of a 15% by mass solution of p-toluenesulfonic acid chloride in triethylamine into the reactor. Keep the mixture at 25°C for 12 hours. Wash the reaction solution 2-3 times with a 3 mol / L hydrochloric acid solution. Combine the organic phases and adjust the pH value to 7 with sodium bicarbonate. Filter and concentrate the filtrate under reduced pressure to obtain esterified polyethylene glycol.

[0028] S3: Add 1 kg of esterified polyethylene glycol and 4 kg of 28% ammonia water into a reactor, keep the reaction at 140°C for 6 hours, extract the reaction product with DCM three times, combine the organic phases, adjust the pH value to 12 with a sodium hydroxide solution with a molar concentration of 1 mol / L, stir at 25°C and 300 r / min for 5 hours, let stand, separate the organic phase and wash with a saturated sodium chloride solution until neutral, filter, dry the filtrate with anhydrous sodium sulfate to remove water, let stand, filter, and concentrate under reduced pressure to obtain amino-terminated polyethylene glycol.

[0029] S4: 100g of amino-terminated polyethylene glycol, 200g of lignin nanoparticles, 1kg of potassium humate and 25kg of deionized water were added to the reactor, stirred at 80 and 300r / min for 40min, and then 2kg of dialdehyde starch, 3kg of anhydrous methanol, 1kg of boric acid, 1kg of glutaraldehyde and 2kg of 10% acetic acid solution were added to the reactor, and stirring was continued for 30min to obtain a composite film-forming solution.

[0030] S5: Due to the need for high-temperature summer treatment, the cultivation site should be selected under the forest or in a shady area. The requirements for the cultivation site are: first, convenient transportation, clean and sufficient water sources, easy operation of the rotary tiller and ventilation; second, the soil layer is deep and rich in humus, and loose loam with convenient irrigation and drainage is preferred; third, edible fungi such as Agaricus bisporus and Dictyophora indica have not been grown within 5 years.

[0031] S6: Collection of undisturbed soil samples: When wild morels are found, collect undisturbed soil samples of 0-20cm within a radius of 50cm around the morels as soon as possible. Only semicircular soil samples are taken, and the semicircular undisturbed soil is retained. After the undisturbed soil is collected, fill it with other soil next to it to minimize the damage to the living environment of wild morels and protect wild morel resources. The amount of undisturbed soil collected is determined according to the number and area of ​​wild morels, and 5-10kg of undisturbed soil is collected.

[0032] S7: (1) Cultivation site preparation: clean up the stones, weeds, agricultural films and other debris in the cultivation site, and spread 60kg of quicklime per mu for disinfection. Deeply turn the soil 25cm-30cm, and control the soil particle size to 1cm-3cm. According to the conditions of the cultivation site, reasonably arrange to dig drainage ditches around it, with a width of 40cm and a depth of 60cm.

[0033] (2) Digging a pit: Based on the weight of the collected undisturbed soil, dig a rectangular pit with a depth of 5 cm, a length of 100 cm, and a width of 50 cm. Reserve an area of ​​50 m for expansion at the back. 2 .

[0034] (3) Cultivation: ① Spring wild morels: After collecting the original soil in the first year, spread it evenly in a rectangular pit to a height of 5-8 cm and heat it at 1.5 kg / m 2 Spray the composite film-forming liquid at a dosage and compact it, cover with a double-layer sunshade net, keep the soil moisture at 25% (the soil becomes dry and white, and water is added appropriately), and undergo summer treatment (cover the sunshade net with a layer of straw, add an arch shed with a height of 30-50cm, and then cover it with another layer of sunshade net); from the end of September to mid-October, when white or light yellow fungal frost is visible on the soil surface, remove the sunshade net and straw on the soil surface, and add nutrient bags (4 bags / m 2 ), in early December, cover with a black film to keep warm and moisturize. The black film must be evenly perforated with small holes of 2-3cm in diameter, with an interval of 25-35cm, to increase its air permeability. In mid-February of the second year, the temperature begins to rise, and the black film is removed. Water is poured thoroughly to stimulate fruiting. Fruiting begins at the end of February to the end of March, and the harvest is over at the end of April. After the harvest, remove all nutrient bags without damaging the original soil, and then cover with a double-layer sunshade net, replace the arch shed and sunshade net, and keep the soil moisture at 20-25% (when the soil becomes dry and white, add water appropriately), and undergo summering treatment (cover the sunshade net with a layer of straw, add an arch shed with a height of 30-50cm, and cover it with another layer of sunshade net); in mid-September, plow the soil 25cm-30cm, and control the soil particle size to 1cm-3cm. From the end of September to mid-October, when white or light yellow fungal frost is visible on the soil surface, remove the sunshade net and straw on the soil surface, and add nutrient bags (4 bags / m 2 ), cover with black film in early December to keep warm and moisturize. The black film must be evenly perforated with small holes of 2-3cm in diameter, 30cm apart, to increase its air permeability. In mid-February of the third year, the temperature starts to rise, remove the black film, water thoroughly to stimulate fruiting, fruiting starts at the end of February to the end of March, and the harvest ends at the end of April. 2-3 flushes of mushrooms can be harvested. The later management is the same as the second year, and so on. The cultivation area is expanded year by year, such as 0.5m in the first year. 2 , can reach 2-3m in the second year 2 , can reach 8m in the third year 2 , can reach 10-15m in the fourth year 2, follow the above operations, maintain soil moisture, maintain mycelium vitality and quality, and continue to expand the area. Avoid spraying pesticides and fertilizers, and clean up weeds in time. The yield in the second year is low, and after the third year, the average yield can reach 0.30kg / m2. ② Autumn wild morels: After collecting the original soil in the first year, evenly spread it in a rectangular pit to a height of 5-8cm, water it thoroughly, build an arch shed with a height of 50cm, cover it with a shade net, and keep the soil moisture at 20-25% (the soil becomes dry and white, and water is added appropriately). 20 days after transplantation, add nutrient bags (6 bags / m 2 ), in early December, cover with black film to keep warm and moisturize. The black film must be evenly perforated with small holes of 2-3cm in diameter, 30cm apart, to increase its air permeability. In March of the second year, when the temperature rises, remove the black film, and then keep the soil moisture at 20-25%. After summering treatment (cover the soil surface with a double layer of sunshade net and a layer of straw, keep the original arch shed and cover it with a layer of sunshade net), until mid-September, when the temperature drops to 25 or below, remove the sunshade net and straw from the soil surface, water thoroughly to stimulate mushroom production, and mushrooms can be produced in early to mid-October. Harvesting is completed in late November to early December. Plough the soil 30cm, and control the soil particle size to 2cm. Then, 12 days later, when white or light yellow fungus frost is visible on the soil surface, add nutrient bags (6 bags / m 2 ), cover with black film (same as above), and then the same as above, and so on. The management is the same as the wild morel in spring. The yield is lower in the second year, and the average yield can reach 0.45kg / m after the third year. 2 .

[0035] Embodiment 2: This embodiment provides a cultivation method using undisturbed soil of Morchella, comprising the following steps:

[0036] S1: 1 kg of ammonium lignin sulfonate and 150 L of deionized water were added to a reactor and stirred to dissolve. 250 L of anhydrous ethanol was added dropwise to the reactor under stirring conditions of 200 r / min to induce the ammonium lignin sulfonate to assemble into lignin nanoparticles with uniform particle size. After the addition was completed, the anhydrous ethanol was removed by rotary evaporation and vacuum dried to obtain lignin nanoparticles.

[0037] S2: Dissolve 2 kg of polyethylene glycol in 10 kg of dichloromethane and transfer the solution to a reactor. Add 5 kg of a 15% by mass solution of p-toluenesulfonic acid chloride in triethylamine dropwise to the reactor. Keep the mixture at 20°C for 10 hours. Wash the reaction solution twice with a 3 mol / L hydrochloric acid solution. Combine the organic phases and adjust the pH value to 7 with sodium bicarbonate. Filter the mixture and concentrate the filtrate under reduced pressure to obtain esterified polyethylene glycol.

[0038] S3: Add 1 kg of esterified polyethylene glycol and 4 kg of 28% ammonia water into a reactor, keep the reaction at 135-140°C for 5 hours, extract the reaction product with DCM three times, combine the organic phases, adjust the pH value to 11.5 with a sodium hydroxide solution with a molar concentration of 1 mol / L, stir for 4-5 hours at 20°C and 200 r / min, let stand, separate the organic phase and wash with a saturated sodium chloride solution until neutral, filter, dry the filtrate with anhydrous sodium sulfate to remove water, let stand, filter, and concentrate under reduced pressure to obtain amino-terminated polyethylene glycol.

[0039] S4: 100g of amino-terminated polyethylene glycol, 200g of lignin nanoparticles, 1kg of potassium humate and 25kg of deionized water are added to a reactor, and stirred at 70 and 200r / min for 30-40min, and then 2kg of dialdehyde starch, 3kg of anhydrous methanol, 1kg of boric acid, 1kg of glutaraldehyde and 2kg of 10% acetic acid solution are added to the reactor, and stirring is continued for 20-30min to obtain a composite film-forming solution.

[0040] S5: In the wild morel mushroom growing area, dig 10cm thick original soil from the surface of the morel mushroom as the cultivation soil; dig a mushroom bed in the cultivation area, transfer the collected original soil from the wild morel mushroom growing area to the dug mushroom bed, and spread it flat according to the pressure of 1kg / m 2 Spray the composite film-forming liquid in appropriate amount and compact it.

[0041] The original soil of wild morels growing ground is rich in morel hyphae and necessary microbial communities and nutrients, which is conducive to the growth of morels.

[0042] S6: placing nutrient bags on the paved original soil for morels, with a usage of 1500kg / mu of nutrient bags, and containing morel fungus grains in the nutrient bags, which are obtained by mixing rice bran and wheat in a mass ratio of 1:3 and then undergoing high-temperature sterilization, and have a water content of 45%.

[0043] S7: During the cultivation process, an arch shed is built, the temperature is controlled at 16° C. and the humidity is controlled at 60%, and the morels are planted under a light flux of 500 lumens. The morels are ventilated every 2 days to avoid mold growth. The morels can be harvested when the cap diameter is 5 cm, thereby completing the cultivation method using the original soil of the morels.

[0044] Embodiment 3: This embodiment provides a cultivation method using undisturbed soil of Morchella, comprising the following steps:

[0045] S1: 1 kg of ammonium lignin sulfonate and 150 L of deionized water were added to a reactor and stirred to dissolve. 280 L of anhydrous ethanol was added dropwise to the reactor under stirring conditions of 250 r / min to induce the ammonium lignin sulfonate to assemble into lignin nanoparticles with uniform particle size. After the addition was completed, the anhydrous ethanol was removed by rotary evaporation and vacuum dried to obtain lignin nanoparticles.

[0046] S2: Dissolve 2 kg of polyethylene glycol in 10 kg of dichloromethane and transfer to a reactor. Add 5.5 kg of a 15% by mass solution of p-toluenesulfonic acid chloride in triethylamine to the reactor. Keep the mixture at 22°C for 11 hours. Wash the reaction solution twice with a 3 mol / L hydrochloric acid solution. Combine the organic phases and adjust the pH value to 7 with sodium bicarbonate. Filter and concentrate the filtrate under reduced pressure to obtain esterified polyethylene glycol.

[0047] S3: Add 1 kg of esterified polyethylene glycol and 4 kg of 28% ammonia water into a reactor, keep the reaction at 138°C for 5.5 hours, extract the reaction product with DCM three times, combine the organic phases, adjust the pH value to 11.8 with a sodium hydroxide solution with a molar concentration of 1 mol / L, stir for 4.5 hours at 22°C and 250 r / min, let stand, separate the organic phase and wash with a saturated sodium chloride solution until neutral, filter, dry the filtrate with anhydrous sodium sulfate to remove water, let stand, filter, and concentrate under reduced pressure to obtain amino-terminated polyethylene glycol.

[0048] S4: 100g of amino-terminated polyethylene glycol, 200g of lignin nanoparticles, 1kg of potassium humate and 25kg of deionized water were added to the reactor, and stirred at 75 and 250r / min for 35min. Then, 2kg of dialdehyde starch, 3kg of anhydrous methanol, 1kg of boric acid, 1kg of glutaraldehyde and 2kg of 10% acetic acid solution were added to the reactor, and stirring was continued for 25min to obtain a composite film-forming solution.

[0049] S5: In the wild morel mushroom growing area, dig 15cm thick original soil from the surface of the morel mushroom as the cultivation soil; dig a mushroom bed in the cultivation area, transfer the collected original soil from the wild morel mushroom growing area to the dug mushroom bed, and spread it flat according to the pressure of 1.2kg / m 2 Spray the composite film-forming liquid in appropriate amount and compact it.

[0050] S6: placing nutrient bags on the paved original soil for morels, with a usage of 1800 kg / mu of nutrient bags, and containing morel fungus grains in the nutrient bags, which are obtained by mixing rice bran and wheat in a mass ratio of 1:3 and then undergoing high-temperature sterilization, and have a water content of 45%.

[0051] S7: During the cultivation process, an arch shed is built, the temperature is controlled at 17° C. and the humidity is 70%, and the morels are planted under a light flux of 650 lumens. Ventilation is performed every 2 days to avoid mold growth. The morels can be harvested when the cap diameter is 6 cm, thereby completing the cultivation method using the original soil of the morels.

[0052] Embodiment 4: This embodiment provides a cultivation method using undisturbed soil of Morchella, comprising the following steps:

[0053] S1: 1 kg of ammonium lignin sulfonate and 150 L of deionized water were added to a reactor and stirred to dissolve. 300 L of anhydrous ethanol was added dropwise to the reactor under stirring at 300 r / min to induce the ammonium lignin sulfonate to assemble into lignin nanoparticles with uniform particle size. After the addition was completed, the anhydrous ethanol was removed by rotary evaporation and vacuum dried to obtain lignin nanoparticles.

[0054] S2: Dissolve 2 kg of polyethylene glycol in 10 kg of dichloromethane and transfer to a reactor. Then, drop 6 kg of a 15% by mass solution of p-toluenesulfonic acid chloride in triethylamine into the reactor. Keep the mixture at 25°C for 12 hours. Wash the reaction solution 2-3 times with a 3 mol / L hydrochloric acid solution. Combine the organic phases and adjust the pH value to 7 with sodium bicarbonate. Filter and concentrate the filtrate under reduced pressure to obtain esterified polyethylene glycol.

[0055] S3: Add 1 kg of esterified polyethylene glycol and 4 kg of 28% ammonia water into a reactor, keep the reaction at 140°C for 6 hours, extract the reaction product with DCM three times, combine the organic phases, adjust the pH value to 12 with a sodium hydroxide solution with a molar concentration of 1 mol / L, stir at 25°C and 300 r / min for 5 hours, let stand, separate the organic phase and wash with a saturated sodium chloride solution until neutral, filter, dry the filtrate with anhydrous sodium sulfate to remove water, let stand, filter, and concentrate under reduced pressure to obtain amino-terminated polyethylene glycol.

[0056] S4: 100g of amino-terminated polyethylene glycol, 200g of lignin nanoparticles, 1kg of potassium humate and 25kg of deionized water were added to the reactor, stirred at 80 and 300r / min for 40min, and then 2kg of dialdehyde starch, 3kg of anhydrous methanol, 1kg of boric acid, 1kg of glutaraldehyde and 2kg of 10% acetic acid solution were added to the reactor, and stirring was continued for 30min to obtain a composite film-forming solution.

[0057] S5: In the wild morel mushroom growing area, dig 20cm thick original soil from the surface of the morel mushroom as the cultivation soil; dig a mushroom bed in the cultivation area, transfer the collected original soil from the wild morel mushroom growing area to the dug mushroom bed, and spread it flat according to the pressure of 1.5kg / m 2Spray the composite film-forming liquid in appropriate amount and compact it.

[0058] S6: placing a nutrient bag on the paved original soil for morels, the amount of the nutrient bag is 2000kg / mu, the nutrient bag is filled with morel fungus grain, the morel fungus grain is obtained by mixing rice bran and wheat in a mass ratio of 1:3 and then undergoing high temperature sterilization, and the water content is 45%.

[0059] S7: During the cultivation process, an arch shed was built, the temperature was controlled at 18°C ​​and the humidity was 75%, and the morels were planted under a light flux of 700 lumens. Ventilation was performed every 2 days to avoid mold growth. The morels were harvested when the cap diameter was 7 cm, completing the cultivation method of morels using undisturbed soil.

[0060] The molecular weight of the polyethylene glycol used in the examples is 1000.

[0061] The cultivation method using the original soil of morels provided by the present invention fully utilizes wild resources, retains the microbial community and nutrients in the original soil of morels, can be used for the growth and reproduction of wild morels, thereby improving the cultivation efficiency of wild morels and protecting wild morel resources.

[0062] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0063] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A cultivation method using undisturbed soil of Morchella, characterized in that: The steps include: Step 1: In the wild morel mushroom growing area, dig 10-20cm thick original soil of the morel, dig a mushroom bed in the cultivation area, transfer the original soil of the morel to the dug mushroom bed, and spread it flat according to 1-1.5kg / m 2 Spray the composite film-forming liquid in the amount and compact it; Step 2: Place a nutrient bag filled with Morchella food on the paved Morchella soil. The amount of the nutrient bag is 1500-2000kg / mu. Step 3: During the cultivation process, an arch shed is built, the temperature is controlled at 16-18° C. and the humidity is controlled at 60-75%, and the morels are planted under a light flux of 500-700 lumens. The morels are ventilated every 2 days and can be harvested when the diameter of the morel cap is 5-7 cm, thereby completing the cultivation method using the original soil of the morels.

2. A cultivation method using undisturbed soil of Morchella according to claim 1, characterized in that: The morel mushroom grain is obtained by mixing rice bran and wheat in a mass ratio of 1:3 and then undergoing high-temperature sterilization. The water content of the morel mushroom grain is 45%.

3. The method for cultivating Morchella virgin soil according to claim 1, characterized in that: The composite film-forming solution is prepared by the following steps: Add amino-terminated polyethylene glycol, lignin nanoparticles, potassium humate and deionized water into a reaction kettle, stir at 70-80 and 200-300 r / min for 30-40 min, then add dialdehyde starch, anhydrous methanol, boric acid, glutaraldehyde and 10 wt% acetic acid solution, stir for 20-30 min to obtain a composite film-forming solution.

4. The method for cultivating Morchella edodes using undisturbed soil according to claim 3, characterized in that: The mass ratio of the amino-terminated polyethylene glycol, lignin nanoparticles, potassium humate, deionized water, dialdehyde starch, anhydrous methanol, boric acid, glutaraldehyde and acetic acid solution is 1:2:10:250:20:30:10:10:

20.

5. The method for cultivating Morchella virgin soil according to claim 3, characterized in that: The lignin nanoparticles are prepared by the following steps: Add ammonium lignin sulfonate and deionized water into a reaction kettle and stir to dissolve, add anhydrous ethanol dropwise under stirring at 200-300 r / min, remove the anhydrous ethanol by rotary evaporation after the addition is complete, and vacuum dry to obtain lignin nanoparticles.

6. The method for cultivating Morchella edodes using undisturbed soil according to claim 5, characterized in that: The usage ratio of the ammonium lignin sulfonate, deionized water and anhydrous ethanol is 0.1 g: 15 mL: 25-30 mL.

7. The method for cultivating Morchella edodes using undisturbed soil according to claim 3, characterized in that: The amino-terminated polyethylene glycol is prepared by the following steps: Add esterified polyethylene glycol and 28 wt% ammonia water into a reactor, keep the reaction at 135-140°C for 5-6 hours, extract the reaction product with DCM three times, combine the organic phases, adjust the pH value to 11.5-12 with 1 mol / L sodium hydroxide solution, stir at 20-25°C and 200-300 r / min for 4-5 hours, let stand, separate the organic phase and wash with saturated sodium chloride solution until neutral, filter, dry the filtrate with anhydrous sodium sulfate to remove water, let stand, filter, and concentrate under reduced pressure to obtain amino-terminated polyethylene glycol.

8. The method for cultivating Morchella edodes using original soil according to claim 7, characterized in that: The mass ratio of the esterified polyethylene glycol to aqueous ammonia is 1:

4.

9. The method for cultivating Morchella edodes using original soil according to claim 7, characterized in that: The esterified polyethylene glycol is prepared by the following steps: Dissolve polyethylene glycol in dichloromethane and transfer it to a reactor. Add 15 wt% p-toluenesulfonic acid chloride triethylamine solution dropwise into the reactor. Keep the reaction at 20-25°C for 10-12 hours. Wash the reaction solution 2-3 times with 3 mol / L hydrochloric acid solution. Combine the organic phases and adjust the pH value to 7 with sodium bicarbonate. Filter and concentrate the filtrate under reduced pressure to obtain esterified polyethylene glycol.

10. The method for cultivating Morchella edodes using undisturbed soil according to claim 9, characterized in that: The mass ratio of the polyethylene glycol, dichloromethane and triethylamine solution of p-toluenesulfonic acid chloride is 2:10:5-6.