Anti-dandruff composition as well as preparation method and application thereof

Abstract

The invention provides an anti-dandruff composition as well as a preparation method and application thereof. The anti-dandruff composition is prepared from the following components in parts by weight: 10 to 80 parts of rhizoma anemarrhenae root extract, 10 to 60 parts of flos sophorae bud extract, 5 to 40 parts of olive leaf extract and 10 to 90 parts of solvent. According to the anti-dandruff composition, a drug-resistant pathway is blocked in a multi-target mode, and compared with a traditional chemical anti-dandruff agent which plays a role through a single target, the anti-dandruff composition has the advantages that the probability of generating drug resistance is lower, and a more lasting anti-dandruff effect can be provided.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to an anti-dandruff composition, a preparation method and application thereof. Background Art

[0002] Under normal circumstances, the amount of keratinocytes shed from the scalp is small and the shedding speed is slow, so it is not obvious. However, under certain stimulation, immature keratinocytes will be produced in large quantities, clump and fall off, forming a "snowflake" phenomenon, which is commonly known as dandruff.

[0003] Dandruff is a common scalp problem caused by excessive proliferation of fungi (such as Malassezia). The anti-dandruff and anti-itching agents commonly used in current anti-dandruff products are mostly artificially synthesized chemicals such as piroctone olamine salt.

[0004] Although existing anti-dandruff products have a certain anti-dandruff effect, long-term use may lead to drug resistance problems, and the anti-dandruff effect is not long-lasting. Summary of the invention

[0005] In view of the deficiencies in the prior art, the purpose of the present invention is to provide an anti-dandruff composition, a preparation method and its application, aiming to solve the technical problems in the prior art of removing dandruff by artificially synthesized chemical substances, which have the problem of drug resistance and the anti-dandruff effect is not long-lasting.

[0006] In order to achieve the above-mentioned purpose, in the first aspect, the present invention provides an anti-dandruff composition, comprising the following components in parts by weight: 1 to 80 parts of Anemarrhena root extract, 1 to 60 parts of Sophora japonica bud extract, 1 to 40 parts of Olive leaf extract and 10 to 90 parts of solvent.

[0007] Furthermore, the invention comprises the following components in parts by weight: 40 to 70 parts of anemarrhena root extract, 30 to 50 parts of sophora japonica bud extract, 20 to 30 parts of olive leaf extract and 20 to 30 parts of solvent.

[0008] Furthermore, the solvent includes at least one of glycerol, butylene glycol, propylene glycol and deionized water.

[0009] In a second aspect, the present invention provides a method for preparing an anti-dandruff composition, which is used to prepare the anti-dandruff composition as described in the first aspect above, and the method for preparing the anti-dandruff composition comprises the following steps:

[0010] The extract of the root of Anemarrhena asphodeloides is mixed with a solvent to obtain a first-stage product, and the first-stage product is subjected to a first stirring treatment to obtain a first standby product;

[0011] Mixing the Sophora japonica bud extract with a solvent to obtain a second-stage product, and performing a second stirring treatment on the second-stage product to obtain a second standby product;

[0012] Mixing the olive leaf extract with a solvent to obtain a third-stage product, and performing a second stirring treatment on the third-stage product to obtain a third standby product;

[0013] The first reserve material, the second reserve material and the third reserve material are mixed into a fourth-stage material, and the fourth-stage material is subjected to a fourth stirring treatment to obtain an anti-dandruff composition.

[0014] In a third aspect, the present invention provides a use of the anti-dandruff composition as described in the first aspect in the preparation of cosmetics.

[0015] Furthermore, the weight proportion of the anti-dandruff composition in the cosmetic is 0.01% to 30%.

[0016] Furthermore, the cosmetic is shampoo.

[0017] Furthermore, the preparation method of the shampoo is:

[0018] Mixing sodium laureth sulfate, lauramidopropyl betaine, cocamide MEA, EDTA-2Na and deionized water to form a first intermediate product;

[0019] The first intermediate product is subjected to a heating treatment, and polyquaternium-10 and a pearlescent agent are added to the first intermediate product to obtain a second intermediate product;

[0020] Cooling the second intermediate product to a first preset temperature, and adding sodium cocoamphoacetate and an acrylic copolymer to the second intermediate product to obtain a third intermediate product;

[0021] The third intermediate product is cooled to a second preset temperature, and the anti-dandruff composition, polydimethylsiloxane, kasone and sodium benzoate are added to obtain shampoo.

[0022] Furthermore, the first preset temperature is 60°C, and the second preset temperature is 40°C.

[0023] Compared with the prior art, the beneficial effects of the present invention are as follows: oleuropein in olive leaves is embedded in the cell membrane of Malassezia through hydrophobic action, and cooperates with timosaponin to form nanoscale pores. If fungi want to develop drug resistance, they need to simultaneously change membrane lipid composition, repair membrane pore structure and other multiple mechanisms, thereby reducing the probability of developing drug resistance. Quercetin in Sophora japonica buds inhibits NADH dehydrogenase activity, and timosaponin mangiferin blocks ATP synthase. The two cooperate to reduce the mitochondrial membrane potential of fungi and completely collapse the energy supply. This double metabolic blow prevents fungi from escaping through reconstruction of a single metabolic pathway. Olive polyphenols inhibit DNA methyltransferase DNMT3A, and genistein in Sophora japonica buds regulates histone acetylation. The two jointly silence the expression of efflux pump gene MDR1 and drug resistance-related gene ERG11, thereby blocking the activation of drug resistance genes from the epigenetic level. The anti-dandruff composition of the present application is active, that is, it blocks the drug resistance pathway through a multi-target approach. Compared with traditional chemical anti-dandruff agents that work through a single target, the probability of drug resistance is lower; the linoleic acid in the Sophora japonica buds promotes the synthesis of ceramide EOS, increases the density of the lipid bilayer of the stratum corneum of the scalp, forms a dense physical barrier, and optimizes the sebum composition at the same time, changing the oil environment on which Malassezia depends for survival. Sophora japonica polysaccharides and proteins form a bioadhesive film, so that active ingredients such as oleuropein and mangiferin are sustained-released in the hair follicles for more than 72 hours, ensuring that fungal resuscitation can be continuously inhibited. The extract of Anemarrhena asphodeloides root induces the continuous high expression of the antimicrobial peptide hBD-2, and the chlorogenic acid of Sophora japonica activates the Nrf2 antioxidant pathway, forming a double protection of "chemical sterilization + immune defense", that is, the anti-dandruff composition of the present application can provide a more lasting anti-dandruff effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 is a flow chart of the preparation method of the anti-dandruff composition in Example 6 of the present invention;

[0025] The following specific implementation manner will further illustrate the present invention in conjunction with the above-mentioned drawings. DETAILED DESCRIPTION

[0026] In order to facilitate the understanding of the present invention, the present invention will be described more fully below with reference to the relevant drawings. Several embodiments of the present invention are given in the drawings. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present invention more thorough and comprehensive.

[0027] It should be noted that when an element is referred to as being "fixed to" another element, it may be directly on the other element or there may be a central element. When an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be a central element at the same time. The terms "vertical", "horizontal", "left", "right" and similar expressions used herein are for illustrative purposes only.

[0028] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art of the present invention. The terms used herein in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more related listed items.

[0029] See also Figure 1 In the first aspect, Example 1 of the present invention provides an anti-dandruff composition, comprising an Anemarrhena root extract, a Sophora japonica bud extract, an Olive leaf extract and a solvent. Specifically, it comprises the following components in parts by weight: 10 parts of Anemarrhena root extract, 10 parts of Sophora japonica bud extract, 5 parts of Olive leaf extract and 10 parts of solvent.

[0030] The extract of the root of Anemarrhena asphodeloides can be prepared by the following method: crushing the root of Anemarrhena asphodeloides decoction pieces into root of Anemarrhena asphodeloides particles, mixing the root of Anemarrhena asphodeloides particles in deionized water at a solid-liquid ratio of 1:10, performing a first reflux extraction at 90° C., the number of the first reflux extraction is 2 times, the time of the first reflux extraction is 1.5 hours, to obtain a first extract and a second extract, mixing the first extract and the second extract into a first mixed extract, filtering and centrifuging the first mixed extract to obtain a first treated liquid, and performing a reduced pressure concentration and drying treatment on the first treated liquid to obtain the root of Anemarrhena asphodeloides extract;

[0031] The sophora japonica bud extract can be prepared by the following method: ultrasonically cleaning, drying and crushing fresh sophora japonica buds to obtain sophora japonica bud particles, mixing the sophora japonica bud particles in a 70% ethanol solution at a solid-liquid ratio of 1:10, performing a second reflux extraction at 90° C. to obtain a third extract and residue, mixing the residue in a 50% ethanol solution at a solid-liquid ratio of 1:8, performing a third reflux extraction at 80° C. to obtain a fourth extract, mixing the third extract and the fourth extract into a second mixed extract, and performing membrane separation, reduced pressure concentration and drying on the second mixed extract to obtain a sophora japonica bud extract;

[0032] The olive leaf extract can be prepared by the following method: washing, chopping, air-drying and crushing olive leaves to obtain olive leaf particles, mixing ethanol and dipotassium hydrogen phosphate into a soaking solution, mixing the olive leaf particles into the soaking solution at a solid-liquid ratio of 1:15, performing ultrasonic extraction and static stratification to obtain a static solution, placing a resin in the static solution for dynamic adsorption, gradient eluting the static solution after dynamic adsorption to obtain an eluate, and performing low-temperature concentration and freeze-drying on the eluate to obtain the olive leaf extract.

[0033] Preferably, the solvent includes at least one of glycerol, butylene glycol, propylene glycol and deionized water. In this embodiment, the solvent includes butylene glycol and deionized water. By using the butylene glycol and the deionized water to form the solvent, the solvent dissolves the effective substance and promotes the penetration of the anti-dandruff composition, making the efficacy of the anti-dandruff composition more excellent.

[0034] Example 2 of the present invention provides an anti-dandruff composition, which is different from the anti-dandruff composition described in Example 1 in that:

[0035] The anti-dandruff composition comprises the following components in parts by weight: 80 parts of anemarrhena root extract, 60 parts of sophora japonica bud extract, 40 parts of olive leaf extract and 90 parts of solvent.

[0036] Example 3 of the present invention provides an anti-dandruff composition, which is different from the anti-dandruff composition described in Example 1 in that:

[0037] The anti-dandruff composition comprises the following components in parts by weight: 40 parts of Anemarrhena root extract, 30 parts of Sophora japonica bud extract, 20 parts of Olea europaea leaf extract and 20 parts of solvent.

[0038] Example 4 of the present invention provides an anti-dandruff composition, which is different from the anti-dandruff composition described in Example 1 in that:

[0039] The anti-dandruff composition comprises the following components in parts by weight: 70 parts of Anemarrhena root extract, 50 parts of Sophora japonica bud extract, 30 parts of Olea europaea leaf extract and 30 parts of solvent.

[0040] Comparative Example 1 of the present invention provides an anti-dandruff composition, which is different from the anti-dandruff composition described in Example 1 in that:

[0041] The anti-dandruff composition comprises the following components in parts by weight: 5 parts of Anemarrhena root extract, 5 parts of Sophora japonica bud extract, 2 parts of Olea europaea leaf extract and 5 parts of solvent.

[0042] Comparative Example 2 of the present invention provides an anti-dandruff composition, which is different from the anti-dandruff composition described in Example 1 in that:

[0043] The anti-dandruff composition comprises the following components by weight: 10 parts of Sophora japonica bud extract, 5 parts of Olea europaea leaf extract and 10 parts of solvent. That is, in this comparative example, the Anemarrhena asphodeloides root extract is not used.

[0044] Comparative Example 3 of the present invention provides an anti-dandruff composition, which is different from the anti-dandruff composition described in Example 1 in that:

[0045] The anti-dandruff composition comprises the following components by weight: 10 parts of Anemarrhena root extract, 5 parts of Olea europaea leaf extract and 10 parts of solvent. That is, in this comparative example, no Sophora japonica bud extract is used.

[0046] Comparative Example 4 of the present invention provides an anti-dandruff composition, which is different from the anti-dandruff composition described in Example 1 in that:

[0047] The anti-dandruff composition comprises the following components by weight: 10 parts of Anemarrhena root extract, 10 parts of Sophora japonica bud extract and 10 parts of solvent. That is, in this comparative example, no olive leaf extract is used.

[0048] Comparative Example 5 of the present invention provides an anti-dandruff composition, which is different from the anti-dandruff composition described in Example 1 in that:

[0049] In this comparative example, the anti-dandruff composition is piroctone olamine salt. That is, in this comparative example, the anti-dandruff composition is artificially synthesized by a traditional preparation method.

[0050] The preparation method of the anti-dandruff composition described in Examples 1 to 4 of the present invention and Comparative Examples 1 to 5 was used to prepare an anti-dandruff composition. The anti-dandruff effects of different anti-dandruff compositions were tested by antibacterial experiments. The test was performed according to the antibacterial ring method. Specifically, the antibacterial effect was evaluated by the diameter of the antibacterial ring. The larger the diameter of the antibacterial ring, the better the antibacterial effect. The test strain was: Malassezia furfur, strain number: ATCC 44344. The test results are shown in Table 1 below:

[0051] Table 1

[0052]

[0053] It can be seen from the above table that the anti-dandruff composition described in Examples 1 to 4 of the present application is compared with the anti-dandruff composition described in Comparative Examples 1 to 5. After the components in the anti-dandruff composition described in Examples 1 to 4 of the present application are mixed, oleuropein in olive leaves is embedded in the Malassezia cell membrane through hydrophobic action, and cooperates with anemarrhena saponins to form nanoscale pores. If the fungus wants to develop drug resistance, it needs to simultaneously change the membrane lipid composition, repair the membrane pore structure and other multiple mechanisms, which reduces the probability of developing drug resistance. The quercetin in Sophora japonica buds inhibits the activity of NADH dehydrogenase, and the anemarrhena mangiferin blocks ATP synthase. The two work together to reduce the mitochondrial membrane potential of the fungus and completely collapse the energy supply. This double metabolic blow prevents the fungus from escaping through the reconstruction of a single metabolic pathway. Olive polyphenols inhibit DNA methyltransferase DNMT3A, and genistein in Sophora japonica buds regulates histone acetylation. The two jointly silence the expression of the efflux pump gene MDR1 and the resistance-related gene ERG11 , blocking the activation of drug-resistant genes from the surface level, that is, blocking the drug-resistant pathway through a multi-target approach. Compared with traditional chemical anti-dandruff agents that work through a single target, the anti-dandruff composition in this application has a lower probability of developing drug resistance; the linoleic acid in the Sophora japonica buds promotes the synthesis of ceramide EOS, increases the density of the lipid bilayer of the stratum corneum of the scalp, forms a dense physical barrier, and optimizes the sebum composition at the same time, changing the oil environment on which Malassezia depends for survival. Sophora japonica polysaccharides and proteins form a bioadhesive film, so that active ingredients such as oleuropein and mangiferin are sustained-released in the hair follicles for more than 72 hours, ensuring that fungal resuscitation can be continuously inhibited. The extract from the root of Anemarrhena asphodeloides induces continuous high expression of the antimicrobial peptide hBD-2, and the chlorogenic acid from Sophora japonica activates the Nrf2 antioxidant pathway, forming a double protection of "chemical sterilization + immune defense", that is, the anti-dandruff composition in this application can provide a more lasting and better anti-dandruff effect than artificially synthesized chemical substances such as piroctone ethanolamine salt. By comparing the results of Examples 1 to 4 and Comparative Examples 2 to 4, it can be seen that the lack of the components has a significant impact on the anti-dandruff effect, and the durability of the anti-dandruff is also seriously reduced. By comparing the results of Examples 1 to 5 and Comparative Example 1, it can be seen that by controlling the weight of each component, a better anti-dandruff effect can be effectively ensured.

[0054] See also Figure 1 In the second aspect, Example 6 of the present invention provides a method for preparing an anti-dandruff composition, which is used to prepare the anti-dandruff composition described in Examples 1 to 5 above, and the method for preparing the anti-dandruff composition comprises the following steps:

[0055] S10: mixing the extract of the root of Anemarrhena asphodeloides with a solvent to obtain a first-stage product, and performing a first stirring treatment on the first-stage product to obtain a first standby product;

[0056] S20: mixing the Sophora japonica bud extract with the solvent to obtain a second-stage product, and performing a second stirring treatment on the second-stage product to obtain a second standby product;

[0057] S30: mixing the olive leaf extract with the solvent to obtain a third-stage product, and performing a second stirring treatment on the third-stage product to obtain a third standby product.

[0058] S40: mixing the first reserve material, the second reserve material and the third reserve material into a fourth-stage material, and performing a fourth stirring process on the fourth-stage material to obtain an anti-dandruff composition.

[0059] In a third aspect, Example 7 of the present invention provides a use of the anti-dandruff composition described in Examples 1 to 5 above in the preparation of cosmetics.

[0060] Preferably, the anti-dandruff composition accounts for 0.01% to 30% by weight in the cosmetic, and the cosmetic is shampoo. The preparation method of the shampoo is:

[0061] Mixing sodium laureth sulfate, lauramidopropyl betaine, cocamide MEA, EDTA-2Na and deionized water to form a first intermediate product;

[0062] The first intermediate product is subjected to a heating treatment, and polyquaternium-10 and a pearlescent agent are added to the first intermediate product to obtain a second intermediate product;

[0063] The temperature of the heating treatment is 80°C to 85°C. In this embodiment, the temperature of the heating treatment is 80°C.

[0064] Cooling the second intermediate product to a first preset temperature, and adding sodium cocoamphoacetate and an acrylic copolymer to the second intermediate product to obtain a third intermediate product;

[0065] The first preset temperature is 60°C.

[0066] Cooling the third intermediate product to a second preset temperature, and adding the anti-dandruff composition, polydimethylsiloxane, kasone and sodium benzoate to obtain shampoo;

[0067] The second preset temperature is 40°C.

[0068] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "examples", "specific examples", or "some examples" means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner.

[0069] The above-mentioned embodiments only express several implementation methods of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the scope of the patent of the present invention. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. An anti-dandruff composition, characterized in that: The invention comprises the following components in parts by weight: 10 to 80 parts of rhizoma anemarrhenae root extract, 10 to 60 parts of sophora japonica bud extract, 5 to 40 parts of olive leaf extract and 10 to 90 parts of solvent.

2. The anti-dandruff composition according to claim 1, characterized in that The invention comprises the following components in parts by weight: 40-70 parts of rhizoma anemarrhenae root extract, 30-50 parts of sophora japonica bud extract, 20-30 parts of olive leaf extract and 20-30 parts of solvent.

3. The anti-dandruff composition according to claim 1 or 2, characterized in that: The solvent includes at least one of glycerol, butylene glycol, propylene glycol and deionized water.

4. A method for preparing an anti-dandruff composition, for preparing the anti-dandruff composition according to any one of claims 1 to 3, characterized in that: The preparation method of the anti-dandruff composition comprises the following steps: The extract of the root of Anemarrhena asphodeloides is mixed with a solvent to obtain a first-stage product, and the first-stage product is subjected to a first stirring treatment to obtain a first standby product; Mixing the Sophora japonica bud extract with a solvent to obtain a second-stage product, and performing a second stirring treatment on the second-stage product to obtain a second standby product; Mixing the olive leaf extract with a solvent to obtain a third-stage product, and performing a second stirring treatment on the third-stage product to obtain a third standby product; The first reserve material, the second reserve material and the third reserve material are mixed into a fourth-stage material, and the fourth-stage material is subjected to a fourth stirring treatment to obtain an anti-dandruff composition.

5. Use of the anti-dandruff composition according to any one of claims 1 to 3 in the preparation of cosmetics.

6. The use according to claim 5, characterized in that: The weight proportion of the anti-dandruff composition in the cosmetic is 0.01% to 30%.

7. The use according to claim 5, characterized in that: The cosmetic is shampoo.

8. The use according to claim 7, characterized in that: The preparation method of the shampoo is: Mixing sodium laureth sulfate, lauramidopropyl betaine, cocamide MEA, EDTA-2Na and deionized water to form a first intermediate product; The first intermediate product is subjected to a heating treatment, and polyquaternium-10 and a pearlescent agent are added to the first intermediate product to obtain a second intermediate product; Cooling the second intermediate product to a first preset temperature, and adding sodium cocoamphoacetate and an acrylic copolymer to the second intermediate product to obtain a third intermediate product; The third intermediate product is cooled to a second preset temperature, and the anti-dandruff composition, polydimethylsiloxane, kasone and sodium benzoate are added to obtain shampoo.

9. The use according to claim 8, characterized in that: The first preset temperature is 60°C, and the second preset temperature is 40°C.

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