Composition for improving gastrointestinal function and preparation method and application thereof

Abstract

The invention discloses a composition for improving gastrointestinal functions as well as a preparation method and application of the composition, and relates to the technical field of traditional Chinese medicines. The composition for improving the gastrointestinal function comprises the following components: 30-40 parts of a traditional Chinese medicine extract, 25-35 parts of embryonic bovine gastrointestinal stem cell polypeptide, 1-5 parts of vitamin B12, 3-8 parts of a yeast extract, 8-15 parts of cellulose, 0.5-1.2 parts of taurine, 5-10 parts of digestive enzyme and 5-10 parts of probiotics. According to the composition for improving the gastrointestinal function, all the components cooperate with one another, gastrointestinal hormones and neuropeptides related to appetite can be adjusted, the microecology of intestinal flora is improved, and digestive system diseases which are caused by diet and take dyspepsia as the main characteristic are treated and prevented.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicines, and in particular to a composition for improving gastrointestinal function, a preparation method and application thereof. Background Art

[0002] The aging of gastrointestinal function and the mechanism of disease occurrence are complex topics in the field of medical research, which covers a variety of factors and intricate mechanisms. As the human body ages, the digestive system undergoes a series of physiological decline changes. Specifically, the contraction force of the esophagus and the tension of the upper esophageal sphincter will gradually weaken, causing the process of food passing through the esophagus to become less smooth. At the same time, the damage resistance of the gastric mucosa will also decrease, making the stomach more susceptible to damage from various factors. In addition, the food capacity of the stomach will decrease and the gastric emptying rate will slow down, which not only affects the efficiency of food digestion, but may also cause stomach discomfort and bloating.

[0003] In the small intestine, a decrease in lactase levels can lead to lactose intolerance and affect the absorption of nutrients. At the same time, the small intestine's ability to absorb water will also weaken, and its power will decrease, further affecting the digestion and absorption of food. As an important part of the digestive system, the liver will have less blood flow and enzyme activity, which will affect the liver's metabolism and detoxification of nutrients.

[0004] In addition, changes in gastrointestinal hormone levels are also one of the important factors in the aging of gastrointestinal function and the occurrence of diseases. Imbalances in gastrointestinal hormones such as motilin (MTL), gastrin (GAS), somatostatin (SS), and 5-hydroxytryptamine (5-HT) may lead to insufficient contraction of gastrointestinal smooth muscle, thereby causing gastrointestinal dysfunction. Abnormalities in gastrointestinal mitochondrial function may also have a negative impact on the contractile ability of gastrointestinal smooth muscle.

[0005] In traditional Chinese medicine theory, gastrointestinal aging and diseases are closely related to the pathological changes of multiple internal organs such as the spleen, stomach, large intestine, small intestine, liver and gallbladder. Traditional Chinese medicine believes that emotional instability, depression and other emotional factors may lead to qi stagnation, which in turn affects the function of the spleen and stomach and induces gastrointestinal diseases. When the spleen and stomach are weak, symptoms such as fullness in the stomach and diarrhea may occur. Imbalance of yin and yang can also lead to gastrointestinal dysfunction, and internal organ dysfunction such as liver qi deficiency, heart qi deficiency, spleen qi deficiency, lung qi deficiency, and kidney qi scorch can directly or indirectly affect the normal function of the gastrointestinal tract. In addition, the invasion of exogenous evil and the poor circulation of qi and blood may also affect the digestion and absorption function of the gastrointestinal tract.

[0006] In the treatment of gastrointestinal aging and diseases, Chinese medicine adopts a comprehensive treatment method. By adjusting diet, regulating emotions, improving living habits, and using Chinese medicine and acupuncture, the normal function of the gastrointestinal tract is restored to achieve the purpose of treating and preventing diseases.

[0007] Based on the above background, the present invention proposes a composition for improving gastrointestinal function, a preparation method and an application thereof. The composition combines traditional Chinese medicine theory and modern medical research results, and aims to comprehensively regulate the gastrointestinal system, improve gastrointestinal function, prevent and treat gastrointestinal diseases, and bring new treatment options and hope to patients with gastrointestinal diseases. Summary of the invention

[0008] In order to solve the above problems, the present invention provides a composition for improving gastrointestinal function and a preparation method and application thereof.

[0009] In a first aspect, the present invention provides a composition for improving gastrointestinal function, wherein the composition for improving gastrointestinal function comprises the following components in parts by weight:

[0010] 30-40 parts of Chinese herbal medicine extract, 25-35 parts of fetal bovine gastrointestinal stem cell polypeptide, 1-5 parts of vitamin B12, 3-8 parts of yeast extract, 8-15 parts of cellulose, 0.5-1.2 parts of taurine, 5-10 parts of digestive enzymes and 5-10 parts of probiotics;

[0011] The Chinese medicine extract is prepared from galangal, charred hawthorn, malt, liquorice, tangerine peel, forsythia and purslane in a weight ratio of (4-5):(7-11):(8-12):(2-5):(2-5):(2-5):(2-5).

[0012] Furthermore, the composition for improving gastrointestinal function comprises the following components in parts by weight:

[0013] 35 parts of Chinese herbal medicine extract, 30 parts of embryonic bovine gastrointestinal stem cell polypeptide, 3 parts of vitamin B12, 5 parts of yeast extract, 10 parts of cellulose, 1 part of taurine, 7.5 parts of digestive enzymes and 7.5 parts of probiotics.

[0014] Furthermore, the Chinese medicine extract is prepared from galangal, charred hawthorn, malt, licorice, tangerine peel, forsythia and purslane in a weight ratio of 5:9:10:3:3:3:3.

[0015] Furthermore, the preparation method of the Chinese medicine extract comprises the following process:

[0016] Wash galangal, charred hawthorn, malt, liquorice, tangerine peel, forsythia and purslane, then crush and mix to obtain a mixture;

[0017] The mixed material is subjected to supercritical fluid extraction, and then the obtained extract is concentrated and dried to obtain the traditional Chinese medicine extract;

[0018] Among them, the working condition parameters of the supercritical fluid extraction include: using water, ethanol and chloroform in a volume ratio of (2-3):(5-8):(1-2) as entrainers, the weight of the entrainer is 0.2-0.4 times the total weight of all medicinal materials, the pressure is 35-40MPa, the temperature is 45-55°C, and the time is 1-2 hours.

[0019] Furthermore, the method for preparing the embryonic bovine gastrointestinal stem cell polypeptide comprises the following steps:

[0020] Tissue source: Gastrointestinal tissues of bovine embryos at 5-7 weeks of development were obtained, mechanically chopped, and then digested with 0.2-0.3 wt% pancreatin for 30-45 minutes, and filtered to obtain a single cell suspension;

[0021] Screening and expansion: DMEM / F12 medium containing 10 wt% fetal bovine serum was used, and 20 ng / mL bFGF and 10 ng / mL EGF were added; CD44+ / Lgr5 were sorted by flow cytometry and placed at 37°C, 5% CO 2 Culture under the same conditions, change the medium every 2 days, and subculture to the third generation for use;

[0022] Exosome collection: When the cell confluence reaches 80%, replace the serum-free medium and culture for 48 hours; collect the supernatant, centrifuge at 2000g for 20min, 10000g for 30min, and 100000g for 70min to remove impurities, and resuspend the exosome precipitate in PBS;

[0023] Preparation of polypeptides: Add 0.1% Triton X-100 and shake for 30 minutes; then filter with a 6kD molecular weight cutoff ultrafiltration membrane to remove large molecular proteins; vacuum concentrate to 10% of the original volume and spray dry to obtain embryonic bovine gastrointestinal stem cell polypeptides.

[0024] Furthermore, the digestive enzymes include salivary amylase, pepsin, pancreatic amylase, pancreatic lipase, trypsin, chymotrypsin, elastase, ribonuclease, deoxyribonuclease, gastric lipase, intestinal lipase, cellulase, lactase, sucrase, maltase, phosphatase or ursodeoxycholic acid; the probiotics include Lactobacillus acidophilus, Bifidobacterium animalis, Bifidobacterium lactis, Lactobacillus rhamnosus, Lactobacillus reuteri, Lactobacillus fermentum or Bifidobacterium breve; and the yeast extract includes FD00-A Angel yeast extract.

[0025] In a second aspect, based on the same inventive concept, the present invention provides a method for preparing the composition for improving gastrointestinal function as described in any one of the first aspects, such as Figure 1 As shown, the preparation method comprises the following steps:

[0026] obtaining a Chinese herbal medicine extract;

[0027] Obtaining embryonic bovine gastrointestinal stem cell polypeptides;

[0028] The traditional Chinese medicine extract, the embryonic bovine gastrointestinal stem cell polypeptide and the remaining components are mixed and crushed, and then sieved to obtain the composition for improving gastrointestinal function.

[0029] In the third aspect, based on the same inventive concept, the present invention provides a composition for improving gastrointestinal function as described in any one of the first aspects or a composition for improving gastrointestinal function prepared by the preparation method of the composition for improving gastrointestinal function as described in any one of the second aspects, and its use in the preparation of products for treating gastrointestinal diseases.

[0030] Furthermore, the gastrointestinal disease includes chronic gastritis.

[0031] Furthermore, the dosage form of the product for treating gastrointestinal diseases includes powder, capsule or liquid.

[0032] The above technical solution provided by the embodiment of the present invention has at least the following advantages compared with the prior art:

[0033] The embodiments of the present invention provide a composition for improving gastrointestinal function, a preparation method and an application thereof. The present invention provides a composition for improving gastrointestinal function, wherein the components cooperate with each other, can regulate gastrointestinal hormones and neuropeptides related to appetite, improve the microecology of intestinal flora, and treat and prevent digestive system diseases mainly characterized by indigestion caused by diet. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with the invention and, together with the description, serve to explain the principles of the invention.

[0035] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, for ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative labor.

[0036] Figure 1 The present invention provides a flow chart of a method for preparing a composition for improving gastrointestinal function according to an embodiment of the present invention.

[0037] Figure 2 The histopathological lesion results of the normal control group in the test example of the present invention Figure 1 .

[0038] Figure 3 The histopathological lesion results of the normal control group in the test example of the present invention Figure 2 .

[0039] Figure 4 The histopathological lesion results of the model control group in the test example of the present invention Figure 1 .

[0040] Figure 5 The histopathological lesion results of the model control group in the test example of the present invention Figure 2 .

[0041] Figure 6 The histopathological lesion results of the intervention experimental group in Example 1 of the test example of the present invention Figure 1 .

[0042] Figure 7 The histopathological lesion results of the intervention experimental group in Example 1 of the test example of the present invention Figure 2 . DETAILED DESCRIPTION

[0043] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0044] Unless otherwise specified, various raw materials, reagents, instruments and equipment used in the present invention can be purchased from the market or prepared by existing methods.

[0045] In a first aspect, the present invention provides a composition for improving gastrointestinal function, wherein the composition for improving gastrointestinal function comprises the following components in parts by weight:

[0046] 30-40 parts of Chinese herbal medicine extract, 25-35 parts of fetal bovine gastrointestinal stem cell polypeptide, 1-5 parts of vitamin B12, 3-8 parts of yeast extract, 8-15 parts of cellulose, 0.5-1.2 parts of taurine, 5-10 parts of digestive enzymes and 5-10 parts of probiotics;

[0047] The Chinese medicine extract is prepared from galangal, charred hawthorn, malt, liquorice, tangerine peel, forsythia and purslane in a weight ratio of (4-5):(7-11):(8-12):(2-5):(2-5):(2-5):(2-5).

[0048] An embodiment of the present invention provides a composition for improving gastrointestinal function. The present invention provides a composition for improving gastrointestinal function. The components work together to regulate gastrointestinal hormones and neuropeptides related to appetite, improve the microecology of intestinal flora, and treat and prevent digestive system diseases caused by diet and characterized by indigestion.

[0049] In some specific embodiments, the composition for improving gastrointestinal function comprises the following components in parts by weight:

[0050] 35 parts of Chinese herbal medicine extract, 30 parts of embryonic bovine gastrointestinal stem cell polypeptide, 3 parts of vitamin B12, 5 parts of yeast extract, 10 parts of cellulose, 1 part of taurine, 7.5 parts of digestive enzymes and 7.5 parts of probiotics.

[0051] Furthermore, the Chinese medicine extract is prepared from galangal, charred hawthorn, malt, licorice, tangerine peel, forsythia and purslane in a weight ratio of 5:9:10:3:3:3:3.

[0052] In some specific embodiments, the preparation method of the Chinese medicine extract comprises the following process:

[0053] Wash galangal, charred hawthorn, malt, liquorice, tangerine peel, forsythia and purslane, then crush and mix to obtain a mixture;

[0054] The mixed material is subjected to supercritical fluid extraction, and then the obtained extract is concentrated and dried to obtain the traditional Chinese medicine extract;

[0055] Among them, the working condition parameters of the supercritical fluid extraction include: using water, ethanol and chloroform in a volume ratio of (2-3):(5-8):(1-2) as entrainers, the weight of the entrainer is 0.2-0.4 times the total weight of all medicinal materials, the pressure is 35-40MPa, the temperature is 45-55°C, and the time is 1-2 hours.

[0056] In some specific embodiments, the method for preparing the embryonic bovine gastrointestinal stem cell polypeptide comprises the following process:

[0057] Tissue source: Gastrointestinal tissues of bovine embryos at 5-7 weeks of development were obtained, mechanically chopped, and then digested with 0.2-0.3 wt% pancreatin for 30-45 minutes, and filtered to obtain a single cell suspension;

[0058] Screening and expansion: DMEM / F12 medium containing 10 wt% fetal bovine serum was used, and 20 ng / mL bFGF and 10 ng / mL EGF were added; CD44+ / Lgr5 were sorted by flow cytometry and placed at 37°C, 5% CO 2 Culture under the same conditions, change the medium every 2 days, and subculture to the third generation for use;

[0059] Exosome collection: When the cell confluence reaches 80%, replace the serum-free medium and culture for 48 hours; collect the supernatant, centrifuge at 2000g for 20min, 10000g for 30min, and 100000g for 70min to remove impurities, and resuspend the exosome precipitate in PBS;

[0060] Preparation of polypeptides: Add 0.1% Triton X-100 and shake for 30 minutes; then filter with a 6kD molecular weight cutoff ultrafiltration membrane to remove large molecular proteins; vacuum concentrate to 10% of the original volume and spray dry to obtain embryonic bovine gastrointestinal stem cell polypeptides.

[0061] In some specific embodiments, the digestive enzyme includes salivary amylase, pepsin, pancreatic amylase, pancreatic lipase, trypsin, chymotrypsin, elastase, ribonuclease, deoxyribonuclease, gastric lipase, intestinal lipase, cellulase, lactase, sucrase, maltase, phosphatase or ursodeoxycholic acid; the probiotic includes Lactobacillus acidophilus, Bifidobacterium animalis, Bifidobacterium lactis, Lactobacillus rhamnosus, Lactobacillus reuteri, Lactobacillus fermentum or Bifidobacterium breve; the yeast extract includes FD00-A Angel yeast extract.

[0062] In some specific embodiments, Lactobacillus acidophilus, Bifidobacterium animalis, Bifidobacterium lactis, Lactobacillus rhamnosus, Lactobacillus reuteri, Lactobacillus fermentum or Bifidobacterium breve can be directly commercially available products, and the specific information is shown in Table 1.

[0063] Table 1

[0064]

[0065] In a second aspect, based on the same inventive concept, the present invention provides a method for preparing the composition for improving gastrointestinal function as described in any one of the first aspects, such as Figure 1 As shown, the preparation method comprises the following steps:

[0066] obtaining a Chinese herbal medicine extract;

[0067] Obtaining embryonic bovine gastrointestinal stem cell polypeptides;

[0068] The Chinese medicine extract, the embryonic bovine gastrointestinal stem cell polypeptide and the remaining components are mixed and crushed,

[0069] The mixture is then sieved to obtain the composition for improving gastrointestinal function.

[0070] In the third aspect, based on the same inventive concept, the present invention provides a composition for improving gastrointestinal function as described in any one of the first aspects or a composition for improving gastrointestinal function prepared by the preparation method of the composition for improving gastrointestinal function as described in any one of the second aspects, and its use in the preparation of products for treating gastrointestinal diseases.

[0071] In some embodiments, the gastrointestinal disease comprises chronic gastritis.

[0072] In some specific embodiments, the dosage form of the product for treating gastrointestinal diseases includes powder, capsule or liquid.

[0073] It should be noted that the components and raw materials involved in the composition for improving gastrointestinal function provided in the embodiments of the present invention, its preparation method and application, unless otherwise specified or specified, can be directly commercially available products or homemade using existing public preparation methods; at the same time, the steps and parameters involved, unless otherwise specified or specified, can be carried out according to the process steps and parameters disclosed in the prior art or directly using existing equipment according to the instruction manual, and the present invention document will not repeat them one by one.

[0074] The present invention will be further described below in conjunction with specific examples. It should be understood that these examples are intended only to illustrate the present invention and are not intended to limit the scope of the present invention. The experimental methods in the following examples that do not specify specific conditions are usually measured according to national standards. If there is no corresponding national standard, then the conditions recommended by the manufacturer are followed.

[0075] Example 1

[0076] This example provides a composition for improving gastrointestinal function, which comprises the following components in parts by weight:

[0077] 35 parts of Chinese herbal medicine extract, 30 parts of fetal bovine gastrointestinal stem cell polypeptide, 3 parts of vitamin B12, 5 parts of yeast extract, 10 parts of cellulose, 1 part of taurine, 7.5 parts of digestive enzymes and 7.5 parts of probiotics;

[0078] The Chinese medicine extract is prepared from galangal, charred hawthorn, malt, licorice, tangerine peel, forsythia and purslane in a weight ratio of 5:9:10:3:3:3:3; the preparation method of the Chinese medicine extract comprises the following steps: cleaning galangal, charred hawthorn, malt, licorice, tangerine peel, forsythia and purslane, then crushing and mixing to obtain a mixture; subjecting the mixture to supercritical fluid extraction, and then concentrating and drying the obtained extract to obtain the Chinese medicine extract; wherein the working condition parameters of the supercritical fluid extraction include: using water, ethanol and chloroform in a volume ratio of 2.5:7:1 as entrainers, the weight amount of the entrainer is 0.3 times the total weight of all medicinal materials, the pressure is 38MPa, the temperature is 50°C, and the time is 1.5 hours;

[0079] The preparation method of the embryonic bovine gastrointestinal stem cell polypeptide comprises the following steps: tissue source: taking the gastrointestinal tissue of the bovine embryo at the 5th to 7th week of development, mechanically chopping it, adding 0.5wt% trypsin to digest it for 40 minutes, and filtering to obtain a single cell suspension; screening and amplification: using DMEM / F12 culture medium containing 10wt% fetal bovine serum, adding 20ng / mL bFGF and 10ng / mL EGF; sorting CD44+ / Lgr5 by flow cytometry, and placing it at 37°C and 5% CO 2 The cells were cultured under the following conditions, the medium was changed every 2 days, and the cells were subcultured to the third generation for standby use; exosome collection: when the cell confluence reached 80%, the serum-free medium was replaced and cultured for 48 hours; the supernatant was collected and centrifuged at 2000g for 20min, 10000g for 30min, and 100000g for 70min to remove impurities, and the exosome precipitate was resuspended in PBS; peptide preparation: 0.1% Triton X-100 was added and lysed by shaking for 30min; then filtered with a 6kD molecular weight cutoff ultrafiltration membrane to remove large molecular proteins; vacuum concentrated to 10% of the original volume and spray dried to obtain embryonic bovine gastrointestinal stem cell peptides;

[0080] The yeast extract is FD00-A Angel yeast extract; the digestive enzyme is a 3:2:2d

[0081] Salivary amylase, pepsin, pancreatic amylase; the probiotics are composed of Lactobacillus acidophilus, Bifidobacterium animalis, Bifidobacterium lactis, Lactobacillus rhamnosus, Lactobacillus reuteri, Lactobacillus fermentum and Bifidobacterium breve in a ratio of 2:1:1:2:3:1:1.5.

[0082] The preparation method of the composition for improving gastrointestinal function comprises the following steps:

[0083] obtaining a Chinese herbal medicine extract;

[0084] Obtaining embryonic bovine gastrointestinal stem cell polypeptides;

[0085] The traditional Chinese medicine extract, the embryonic bovine gastrointestinal stem cell polypeptide and the remaining components are mixed and crushed, and then passed through a 200-mesh sieve to obtain the composition for improving gastrointestinal function.

[0086] Example 2

[0087] This example provides a composition for improving gastrointestinal function, which comprises the following components in parts by weight:

[0088] 30 parts of Chinese herbal medicine extract, 25 parts of fetal bovine gastrointestinal stem cell polypeptide, 1 part of vitamin B12, 3 parts of yeast extract, 8 parts of cellulose, 0.5 parts of taurine, 5 parts of digestive enzymes and 5 parts of probiotics;

[0089] The Chinese herbal medicine extract is prepared from galangal, charred hawthorn, malt, liquorice, tangerine peel, forsythia and purslane in a weight ratio of 4:7:8:2:2:2:2; the preparation method of the Chinese herbal medicine extract is the same as that in Example 1;

[0090] The preparation method of the embryonic bovine gastrointestinal stem cell polypeptide is the same as that in Example 1;

[0091] The yeast extract is the same as that in Example 1; the digestive enzyme is the same as that in Example 1; and the probiotics are the same as those in Example 1.

[0092] Example 3

[0093] This example provides a composition for improving gastrointestinal function, which comprises the following components in parts by weight:

[0094] 40 parts of Chinese herbal medicine extract, 30 parts of fetal bovine gastrointestinal stem cell polypeptide, 5 parts of vitamin B12, 8 parts of yeast extract, 15 parts of cellulose, 1.2 parts of taurine, 10 parts of digestive enzymes and 10 parts of probiotics;

[0095] The Chinese herbal medicine extract is prepared from galangal, charred hawthorn, malt, liquorice, tangerine peel, forsythia and purslane in a weight ratio of 5:11:12:5:5:5:5; the preparation method of the Chinese herbal medicine extract is the same as that in Example 1;

[0096] The preparation method of the embryonic bovine gastrointestinal stem cell polypeptide is the same as that in Example 1;

[0097] The yeast extract is the same as that in Example 1; the digestive enzyme is the same as that in Example 1; and the probiotics are the same as those in Example 1.

[0098] Comparative Example 1

[0099] This example provides a composition for improving gastrointestinal function and a preparation method thereof, which is different from Example 1 only in that the amount of the Chinese medicine extract is adjusted to 0 part and the amount of the embryonic bovine gastrointestinal stem cell polypeptide is adjusted to 0 part; the remaining steps and parameters are the same.

[0100] Comparative Example 2

[0101] This example provides a composition for improving gastrointestinal function and a preparation method thereof, which is different from Example 1 only in that the amount of the Chinese medicine extract is adjusted to 0 parts; the remaining steps and parameters are the same.

[0102] Comparative Example 3

[0103] This example provides a composition for improving gastrointestinal function and a preparation method thereof, which is different from Example 1 only in that the amount of embryonic bovine gastrointestinal stem cell polypeptide is adjusted to 0; the remaining steps and parameters are the same.

[0104] Test Example 1

[0105] This example investigates the therapeutic effect of the composition obtained from Example 1 and Comparative Examples 1 to 3 on the bile-induced chronic gastritis model.

[0106] Test method:

[0107] 1. Experimental Animals

[0108] Animal selection: A total of 55 male SD rats aged 6-8 weeks were selected.

[0109] Grouping method: After quarantine, the rats were randomly divided into three groups according to their body weight: normal control group (5 rats), model control group (10 rats), Example 1 intervention treatment experimental group (10 rats), Comparative Example 1 intervention treatment experimental group (10 rats), Comparative Example 2 intervention treatment experimental group (10 rats), Comparative Example 3 intervention treatment experimental group (10 rats). There was no significant difference in body weight among the groups.

[0110] 2. Modeling method

[0111] Preparation of modeling agent: Mix human gallbladder bile and glycerol in a ratio of 1:1 (V / V).

[0112] Gavage method: 4 ml of the mixed solution was gavaged into the model group and the experimental group once a week for 10 weeks. To ensure the consistency of the modeling effect, the gavage operation should be performed by the same person and the gavage time should be fixed.

[0113] 3. Intervention and treatment measures

[0114] Intragastric dose and frequency: The experimental group rats were intragastrically administered with the composition for intervention treatment at a dose of 1 ml / 100 g body weight daily. At the same time, the drinking water of the experimental group was replaced with drinking water containing the composition, which was prepared by weighing 30 g of the composition and dissolving it in 200 ml of drinking water to ensure consistent drug concentration.

[0115] Control group treatment: Rats in the normal control group and the model control group were given pure water by gavage every day and were raised normally without adding any drugs.

[0116] Intervention duration: The intervention lasted for 21 days, and relevant data were recorded.

[0117] 4. Test results

[0118] The results of serum inflammation detection are shown in Table 2.

[0119] Table 2

[0120] Test Group IL-6 content (pg / mL) Normal control group 656.81±47.92 Model control group 2219.52±263.45 Example 1 Intervention Experimental Group 718.75±56.64 Comparative Example 1 Intervention Experimental Group 1570.36±179.48 Comparative Example 2 Intervention Experimental Group 1186.99±135.70 Comparative Example 3: Intervention Group 1267.48±150.19

[0121] The results of serum inflammation detection are shown in Table 3. The scoring criteria in Table 3 are as follows: mucosal injury: 0 no injury, 1 mild injury, 2 moderate injury, 3 severe injury; gastric mask thickness: 0 no atrophy, 1 mild atrophy, 2 moderate atrophy, 3 severe atrophy; inflammatory cell infiltration: 0 none, 1 mild, 2 moderate, 3 severe.

[0122] Table 3

[0123]

[0124] In addition, the histopathological lesions of the normal control group were Figure 2 (HE 40×) and Figure 3 (HE 200×) as shown; the histopathological lesions of the model control group are shown Figure 4 (HE 40×) and Figure 5 (HE 200×) as shown; the histopathological lesions of the intervention experimental group in Example 1 are shown Figure 6 (HE 40×) and Figure 7 (HE 200×). By comparing the above histopathological lesion results, it can be seen that after the intervention treatment with the composition provided in Example 1 of the present invention, the gastric mucosal tissue was covered by columnar epithelium, the glands were not lost, the number of glands was not significantly reduced, no obvious edema and interstitial fibrosis were observed, and a small amount of inflammatory cell infiltration was observed, indicating that it has a significant therapeutic improvement effect on rats with bile-induced chronic gastritis.

[0125] In summary, the embodiments of the present invention provide a composition for improving gastrointestinal function, a preparation method and an application thereof. The present invention provides a composition for improving gastrointestinal function, wherein the components cooperate with each other, can regulate gastrointestinal hormones and neuropeptides related to appetite, improve the microecology of intestinal flora, and treat and prevent digestive system diseases mainly characterized by indigestion caused by diet.

[0126] Various embodiments of the present invention may be presented in the form of a range; it should be understood that the description in the form of a range is only for convenience and brevity, and should not be understood as a rigid limitation on the scope of the present invention; therefore, the range description should be considered to have specifically disclosed all possible sub-ranges and single numerical values ​​within the range. For example, the range description from 1 to 6 should be considered to have specifically disclosed sub-ranges, such as from 1 to 3, from 1 to 4, from 1 to 5, from 2 to 4, from 2 to 6, from 3 to 6, etc., as well as single numbers within the range, such as 1, 2, 3, 4, 5 and 6, which apply regardless of the range. In addition, whenever a numerical range is indicated herein, it is meant to include any cited number (fractional or integer) within the indicated range.

[0127] The foregoing is merely a specific embodiment of the present invention, which enables those skilled in the art to understand or implement the present invention. Various modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features claimed herein.

Claims

1. A composition for improving gastrointestinal function, characterized in that: The composition for improving gastrointestinal function comprises the following components in parts by weight: 30-40 parts of Chinese herbal medicine extract, 25-35 parts of fetal bovine gastrointestinal stem cell polypeptide, 1-5 parts of vitamin B12, 3-8 parts of yeast extract, 8-15 parts of cellulose, 0.5-1.2 parts of taurine, 5-10 parts of digestive enzymes and 5-10 parts of probiotics; The Chinese medicine extract is prepared from galangal, charred hawthorn, malt, liquorice, tangerine peel, forsythia and purslane in a weight ratio of (4-5):(7-11):(8-12):(2-5):(2-5):(2-5):(2-5).

2. The composition for improving gastrointestinal function according to claim 1, characterized in that: The composition for improving gastrointestinal function comprises the following components in parts by weight: 35 parts of Chinese herbal medicine extract, 30 parts of embryonic bovine gastrointestinal stem cell polypeptide, 3 parts of vitamin B12, 5 parts of yeast extract, 10 parts of cellulose, 1 part of taurine, 7.5 parts of digestive enzymes and 7.5 parts of probiotics.

3. The composition for improving gastrointestinal function according to claim 1, characterized in that: The traditional Chinese medicine extract is prepared from galangal, charred hawthorn, malt, liquorice, tangerine peel, forsythia and purslane in a weight ratio of 5:9:10:3:3:3:

3.

4. The composition for improving gastrointestinal function according to claim 1, characterized in that: The preparation method of the Chinese medicine extract comprises the following steps: Wash galangal, charred hawthorn, malt, liquorice, tangerine peel, forsythia and purslane, then crush and mix to obtain a mixture; The mixed material is subjected to supercritical fluid extraction, and the obtained extract is then concentrated and dried to obtain the traditional Chinese medicine extract; Among them, the working condition parameters of the supercritical fluid extraction include: using water, ethanol and chloroform in a volume ratio of (2-3):(5-8):(1-2) as entrainers, the weight of the entrainer is 0.2-0.4 times the total weight of all medicinal materials, the pressure is 35-40MPa, the temperature is 45-55°C, and the time is 1-2 hours.

5. The composition for improving gastrointestinal function according to claim 1, characterized in that: Preparation method of embryonic bovine gastrointestinal stem cell polypeptide The process includes: Tissue source: Gastrointestinal tissues of bovine embryos at 5-7 weeks of development were obtained, mechanically chopped, and then digested with 0.2-0.3 wt% pancreatin for 30-45 minutes, and filtered to obtain a single cell suspension; Screening and expansion: Use DMEM / F12 medium containing 10wt% fetal bovine serum, add 20ng / mL bFGF and 10ng / mL EGF; sort CD44+ / Lgr5 by flow cytometry, culture at 37℃, 5% CO2, change the medium every 2 days, and pass to the third generation for use; Exosome collection: When the cell confluence reaches 80%, replace the serum-free medium and culture for 48 hours; collect the supernatant, centrifuge at 2000g for 20min, 10000g for 30min, and 100000g for 70min to remove impurities, and resuspend the exosome precipitate in PBS; Preparation of polypeptides: Add 0.1% Triton X-100 and shake for 30 minutes; then filter with a 6kD molecular weight cutoff ultrafiltration membrane to remove large molecular proteins; vacuum concentrate to 10% of the original volume and spray dry to obtain embryonic bovine gastrointestinal stem cell polypeptides.

6. The composition for improving gastrointestinal function according to claim 1, characterized in that: The digestive enzymes include salivary amylase, pepsin, pancreatic amylase, pancreatic lipase, trypsin, chymotrypsin, elastase, ribonuclease, deoxyribonuclease, gastric lipase, intestinal lipase, cellulase, lactase, sucrase, maltase, phosphatase or ursodeoxycholic acid; the probiotics include Lactobacillus acidophilus, Bifidobacterium animalis, Bifidobacterium lactis, Lactobacillus rhamnosus, Lactobacillus reuteri, Lactobacillus fermentum or Bifidobacterium breve; the yeast extract includes FD00-A Angel yeast extract.

7. A method for preparing the composition for improving gastrointestinal function according to any one of claims 1 to 6, characterized in that: The preparation method comprises the following steps: obtaining a Chinese herbal medicine extract; Obtaining embryonic bovine gastrointestinal stem cell polypeptides; The traditional Chinese medicine extract, the embryonic bovine gastrointestinal stem cell polypeptide and the remaining components are mixed and crushed, and then sieved to obtain the composition for improving gastrointestinal function.

8. Use of the composition for improving gastrointestinal function according to any one of claims 1 to 6 or the composition for improving gastrointestinal function prepared by the method for preparing the composition for improving gastrointestinal function according to claim 7 in preparing products for treating gastrointestinal diseases.

9. The use according to claim 8, characterized in that: The gastrointestinal diseases include chronic gastritis.

10. The use according to claim 8, characterized in that: The dosage form of the product for treating gastrointestinal diseases includes powder, capsule or liquid.

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