Efficient platelet-rich plasma preparation device and method
By designing a platelet-rich plasma preparation device including a red blood cell compartment, a supernatant compartment and a platelet-rich plasma compartment, combined with a blood drainage column and an anticoagulation coating technology, the problem of difficult to uniformly unify the platelet concentration and mix red blood cells in the prior art is solved, and efficient and standardized platelet-rich plasma preparation is achieved.
Patent Information
- Application Number
- CN202510269616.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-07
- Publication Date
- 2025-06-06
AI Technical Summary
The existing platelet-rich plasma preparation methods have difficulty in unifying the platelet concentration and large differences in the amount of red blood cells and white blood cells, which lead to the difficulty of standardized treatment and clinical efficacy evaluation.
An efficient platelet-rich plasma preparation device was designed, including a red cell compartment, a supernatant compartment and a platelet-rich plasma compartment. Using technical means such as blood drainage column and anticoagulation coating, it effectively removes red blood cells and increases the platelet concentration through high-speed centrifugation and rotation design.
The unified platelet concentration was achieved, the red blood cell removal rate reached 99.9%, which improved the quality of the prepared PRP and was suitable as the standardized basis for clinical treatment.
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Figure CN120094757A_ABST
Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of cell biology, and in particular relates to an efficient preparation device and method of platelet-rich plasma. Background Art
[0002] Platelets are anucleate cells in mammalian blood, smaller than red blood cells and white blood cells. In addition to providing coagulation during hemostasis, they can also release a large number of cell growth factors through the degranulation of intracellular α particles, including platelet-derived growth factor (PDGF), transforming growth factor (TGF-P), insulin-like growth factor-1 (IGF-1), fibroblast growth factor (FGF), vascular endothelial growth factor (VEGF), etc., which can induce the division and proliferation of bone cells and collagen synthesis, and promote the repair and reconstruction of bone tissue. Among them, PDGF can promote the chemotaxis and proliferation of osteoblasts and increase the ability to synthesize collagen; TGF-P has the effect of stimulating the chemotaxis and proliferation of osteoblast precursor cells and osteoblasts, inhibiting osteoclast formation and bone resorption; IGF-I can increase osteoblast activity and promote the formation of cartilage and bone matrix; VEGF can promote the formation of new blood vessels, which is beneficial to the supply of nutrients in the lesion area, and can also directly act on osteoblasts and chondrocytes to enhance their migration, proliferation and cell activity.
[0003] Platelets can be extracted from the patient's own whole blood and play a therapeutic role after enrichment. They have the characteristics of abundant sources, convenient material collection, and simple separation. They are widely used in orthopedics, dentistry, sports medicine and other fields. The existing manual preparation method of platelets is the platelet-rich plasma (PRP) method, and PRP is used for experimental research and clinical treatment. Platelet-rich plasma (PRP) is a plasma containing high concentrations of platelets obtained by ex vivo treatment of autologous peripheral blood and separation with the help of different centrifugal forces. PRP is derived from platelets and contains rich growth factors and proteins, such as platelet-derived growth factor, transforming growth factor β, vascular endothelial growth factor, epidermal growth factor and insulin-like growth factor; these growth factors play an important role in promoting cell proliferation, redifferentiation, chemotaxis and stimulating angiogenesis and repair.
[0004] There are many methods for preparing autologous platelet-rich plasma, but the preparation methods and quality standards are different. It is difficult to unify the platelet concentration in the obtained PRP, and the amount of red blood cells and white blood cells mixed in varies greatly, which is not conducive to standardized treatment and difficult to serve as an analytical basis for clinical efficacy evaluation. In particular, the red blood cells in the preparation of platelet-rich plasma will affect tissue and cartilage repair and play an opposite role in clinical treatment. For example, the red blood cells in PRP cannot be absorbed in the joint cavity, and the accumulation of a large number of red blood cells will cause hemorrhage in the joint cavity and induce the occurrence of arthritis in patients. Based on the potential harmful effects of red blood cells on the activity of PRP, the mixing of red blood cells should be minimized during the preparation of PRP.
[0005] In view of this, it is very necessary to invent an efficient platelet-rich plasma preparation device and method. Summary of the invention
[0006] In order to solve the above technical problems, the present invention provides an efficient platelet-rich plasma preparation device and method, so as to solve the problem that there are many existing methods for platelet-rich plasma, but their preparation methods and quality standards are different, the platelet concentration in the obtained PRP is difficult to unify, and the amount of mixed red blood cells and white blood cells varies greatly, which is not conducive to standardized treatment and is difficult to serve as an analytical basis for clinical efficacy evaluation.
[0007] A highly efficient platelet-rich plasma preparation device comprises a red blood cell compartment, a supernatant compartment, a platelet-rich plasma compartment, a silicone ring, a lower compartment cover, a blood drainage column, a silicone plug, a silicone coating layer, a silicone ring, an anticoagulant coating inside and outside the drainage column, a sealing ring and a blood outlet, wherein the red blood cell compartment, the supernatant compartment and the platelet-rich plasma compartment form three interconnected cavities; the supernatant compartment is threadedly connected to the upper part of the red blood cell compartment; the platelet-rich plasma compartment is located inside the supernatant compartment and is connected to the interior of the supernatant compartment; the lower compartment cover is threadedly connected to the upper end of the platelet-rich plasma compartment ; The blood drainage column is arranged in the middle position inside the supernatant chamber; the silicone plug is filled in the upper end of the inner part of the platelet-rich plasma chamber; the silicone coating layer is arranged on the lower side of the inner part of the platelet-rich plasma chamber; the silicone ring is threadedly connected to the upper outer part of the supernatant chamber; the inner and outer anticoagulant coatings of the drainage column are coated on the outside of the blood drainage column; the sealing ring is glued to the intersection between the red blood cell chamber, the supernatant chamber and the platelet-rich plasma chamber; the blood outlet is integrated and opened at the lower part of the blood drainage column; the side hole of the drainage column is connected to the inside of the blood outlet.
[0008] Preferably, a support area structural surface is integrally provided on the lower outer side of the platelet-rich plasma compartment.
[0009] Preferably, a drainage column side hole is opened on the left side of the upper part of the blood drainage column; and the blood drainage column is transparent.
[0010] Preferably, the two end plugs of the blood drainage column are designed with a medical silica gel coating layer.
[0011] Preferably, the interior of the supernatant chamber has a structure for supporting a blood drainage column, and the supporting structure is designed to have an inclination angle of 5° between the upper and lower contact surfaces.
[0012] In addition, the present invention also provides a method for producing high-efficiency platelet-rich plasma, which specifically comprises the following steps:
[0013] Step 1: Inject the drawn venous whole blood into the preparation device through a syringe. The injection needle is tilted to touch the side hole of the drainage column, and the blood is gently pushed along the side hole of the drainage column through the inner cavity to flow into the red blood cell compartment. This method of injecting blood can effectively avoid blood splashing caused by the current blood injection and blood hanging on the wall.
[0014] Step 2. Through the above-mentioned device design and the preparation method, the initial centrifugation parameters are set to high-speed centrifugation and short-time conditions. The red blood cell components with a specific gravity exceeding 1.08 are settled to the bottom by re-spinning, and then the red blood cell compartment is rotated and the end of the blood drainage column is used to isolate and remove the red blood cells in the plasma layer.
[0015] Preferably, the centrifugal speed is 3000-3500 r / min and the time is 3-5 min.
[0016] Preferably, the tilt angle is 40-50° downward.
[0017] Preferably, the red blood cell removal rate is greater than 99.9%.
[0018] Compared with the prior art, the present invention has the following beneficial effects:
[0019] 1. The inner cavity of the efficient platelet-rich plasma preparation device is provided with a blood drainage column; the drainage column is used as a blood injection preparation device, and mainly plays a role of guiding drainage. The drawn venous whole blood is injected into the preparation device through a syringe, and the injection needle is tilted downward at an angle of 45 degrees to touch the side hole of the drainage column, and the blood is gently pushed along the side hole of the drainage column through the inner cavity and flows into the red blood cell compartment. This blood injection method can effectively avoid blood splashing caused by the current blood injection, causing blood to stick to the wall.
[0020] 2. Side holes are designed at both ends of the blood drainage column to increase the blood flow rate; side incisions are designed near the blocking position at the upper and lower ends of the drainage column to facilitate blood injection and the centrifugal process. The blood is discharged in time. The blood drainage column is designed to be transparent, which is convenient for observing the flow of blood on the inner wall of the drainage column and timely judging whether there is blood hanging on the wall.
[0021] 3. The preparation device is designed to reduce the structural area of the support area. At the same time, the support area is designed with an inclination angle on the upper and lower surfaces. The uneven thickness design can effectively reduce the dead space area in the device to avoid the presence of injected blood hanging on the wall, resulting in incomplete removal of red blood cells.
[0022] 4. The inner and outer surfaces of the blood drainage column are coated with an anticoagulant coating to prevent the fluid friction formed by the blood flow on the inner surface of the preparation device from triggering abnormal coagulation mechanisms and thus forming thrombi. The anticoagulant coating can effectively prevent blood cells from rupturing and aggregating before and after centrifugation. The blood drainage column can be coated by infiltrating it into the anticoagulant coating solution.
[0023] 5. The plugs at both ends of the blood drainage column are coated with medical silicone. The good elasticity of silicone can be used to completely close the chamber of the blood drainage column.
[0024] To summarize, the lock core in the traditional preparation device only has the function of sealing the red blood cell compartment and the platelet-rich plasma compartment. The injected blood flows down along the outer wall of the lock core, and the injection pressure can easily splash the blood into the dead space area of the inner wall of the device, resulting in the red blood cells being unable to settle to the red blood cell compartment at the bottom of the device during the initial centrifugation process, thereby causing the remaining blood to be centrifuged into the platelet-rich plasma compartment during the secondary inverted centrifugation process, resulting in residual red blood cells in the prepared PRP, which is not conducive to clinical treatment effects. In this device, side holes are designed at both ends of the blood drainage column, and blood is injected into the internal cavity structure of the drainage column from the upper side holes. The inner cavity of the drainage column is designed with an anti-coagulant coating, and the inner cavity is smooth, and blood does not stick to the wall or remain. BRIEF DESCRIPTION OF THE DRAWINGS
[0025] Figure 1 It is a structural schematic diagram of the present invention.
[0026] Figure 2 It is a schematic diagram of the internal structure of the present invention.
[0027] Figure 3 2 is a graph showing the results of red blood cell removal rate in an embodiment of the present invention.
[0028] In the figure:
[0029] 1. Red blood cell compartment; 2. Supernatant compartment; 3. Platelet-rich plasma compartment; 4. Silicone ring; 5. Lower compartment cover; 6. Blood drainage column; 7. Silicone plug; 8. Silicone coating layer; 9. Silicone ring; 10. Anticoagulant coating inside and outside the drainage column; 11. Sealing ring; 12. Blood outlet. DETAILED DESCRIPTION
[0030] The present invention is further described below in conjunction with the accompanying drawings:
[0031] Embodiment 1:
[0032] As attached Figure 1 To Attachment Figure 2 As shown, the present invention provides an efficient platelet-rich plasma preparation device, including a red blood cell compartment 1, a supernatant compartment 2, a platelet-rich plasma compartment 3, a silicone ring 4, a lower compartment cover 5, a blood drainage column 6, a silicone plug 7, a silicone coating layer 8, a silicone ring 9, an anticoagulant coating 10 inside and outside the drainage column, a sealing ring 11 and a blood outlet 12, wherein the red blood cell compartment 1, the supernatant compartment 2 and the platelet-rich plasma compartment 3 form three interconnected cavities; the supernatant compartment 2 is threadedly connected to the upper part of the red blood cell compartment 1; the platelet-rich plasma compartment 3 is located inside the supernatant compartment 2 and is connected to the inside of the supernatant compartment 2; the lower compartment cover 5 is threadedly connected to the platelet-rich plasma compartment 3. The upper end of the chamber 3; the blood drainage column 6 is arranged in the middle position of the interior of the supernatant chamber 2; the silicone plug 7 is filled in the upper end of the interior of the platelet-rich plasma chamber 3; the silicone coating layer 8 is arranged on the lower side of the interior of the platelet-rich plasma chamber 3; the silicone ring 9 is threadedly connected to the upper outer part of the supernatant chamber 2; the inner and outer anticoagulant coatings 10 of the drainage column are coated on the outside of the blood drainage column 6; the sealing ring 11 is glued to the intersection between the red blood cell chamber 1, the supernatant chamber 2 and the platelet-rich plasma chamber 3; the blood outlet 12 is integrated at the lower part of the blood drainage column 6; the side hole of the drainage column is connected to the inside of the blood outlet 12.
[0033] In the above embodiment, specifically, a support area structural surface is integrally provided on the lower outer side of the platelet-rich plasma compartment 3 .
[0034] In the above embodiment, specifically, a drainage column side hole is opened on the left side of the upper part of the blood drainage column 6; and the blood drainage column 6 is transparent.
[0035] In the above embodiment, specifically, both ends of the blood drainage column 6 are blocked with a medical silica gel coating layer 8 .
[0036] In the above embodiment, specifically, the interior of the supernatant chamber 2 has a structure for supporting a blood drainage column, and the supporting structure is designed to have an inclination angle of 5° between the upper and lower contact surfaces.
[0037] The working principle of the present invention is as follows: blood is injected into the side hole of the blood drainage column 6 through a syringe, and flows along the inner cavity of the blood drainage column 6. The blood drainage column 6 extends from the puncture position to the red blood cell compartment 1, and is used to connect the three compartments, which can not only achieve blood drainage, but also seal the platelet-rich plasma compartment 3 and the red blood cell compartment 1. The blood drainage column 6 is made of a transparent polymer material, and the two ends of the entity are sealed with a silica gel coating layer 8. The good elastic deformation and hydrophobicity of the silica gel can well seal and isolate the red blood cell layer after the initial centrifugation, and prevent the leakage of the red blood cell layer under high-speed centrifugal force in the secondary inverted centrifugation. There is a structure supporting the blood drainage column in the supernatant compartment 2 of the preparation device. The support structure is designed to have an inclination angle of 5° on the upper and lower contact surfaces. The design of the inclination angle can solve the problem of better blood circulation, and can also solve the problem of rapid separation of the plasma layer after the secondary centrifugation, thereby improving the preparation efficiency. The good circulation also avoids cell rupture. The design can effectively reduce the dead space area in the device and improve the utilization efficiency of blood.
[0038] In addition, the inner and outer surfaces of the blood drainage column 6 are designed with an anticoagulant coating, which reduces the adsorption of blood proteins and blood cells on the product surface, thereby reducing the degree of coagulation reaction. The blood drainage column is immersed in the anticoagulant coating solution, and the coating is solidified on the surface of the blood drainage column at a fixed temperature.
[0039] The plugging ports at both ends of the blood drainage column are tightly wrapped with a thin-walled silicone layer at the upper and lower ends of the drainage column through a secondary encapsulation injection molding process. The good elastic deformation of silicone can be used to seal the red blood cell compartment and the platelet-rich plasma compartment of the blood drainage column.
[0040] Example 2
[0041] The present embodiment provides an efficient platelet-rich plasma method, comprising the following steps: Step 1, injecting the drawn venous whole blood into the preparation device through a syringe, the injection needle is tilted downward at 45 degrees to touch the side hole of the drainage column, and the injected blood flows through the inner cavity along the side hole of the drainage column into the red blood cell compartment;
[0042] Step 2: Centrifuge at a speed of 3000 r / min for 5 min to settle the red blood cell components with a specific gravity > 1.08 at the bottom, and then rotate the red blood cell compartment to achieve a red blood cell removal rate of 99.9%. Figure 3 shown.
[0043] Example 3
[0044] This embodiment provides an efficient platelet-rich plasma method, comprising the following steps:
[0045] Step 1: Inject the drawn venous whole blood into the preparation device through a syringe. The injection needle is tilted downward at 45 degrees to touch the side hole of the drainage column. The injected blood flows along the side hole of the drainage column through the inner cavity into the red blood cell compartment, effectively eliminating the problem of blood residue during the blood injection process.
[0046] Step 2: Centrifuge at a speed of 3300r / min for 5 minutes to settle the red blood cell components with a specific gravity > 1.08 at the bottom. Then, by rotating the red blood cell compartment, the silica gel layer at the lower end of the blood drainage column can be used to seal the red blood cell compartment. The good sealing structure can achieve complete sealing of the red blood cells after the initial centrifugation, effectively eliminating the risk of inverted red blood cells in the secondary centrifugation. The secondary centrifugation can effectively prepare PRP with an enrichment multiple of 4-6.7 times, and a red blood cell removal rate of 99.9%. Therefore, under the above-mentioned initial centrifugation parameters, the red blood cells in the whole blood can be effectively completely settled and the white film layer can be collected. Experimental data on red blood cell removal rate in PRP prepared under the initial centrifugation parameters;
[0047] Initial centrifugal speed Centrifugation time Enrichment multiple Red blood cell removal rate % 1500r 5min 2.6 times 93％ 1790r 5min 4.2 times 94％ 3300r 5min 5.5 times 99.9％
[0048] Example 4
[0049] This embodiment provides an efficient platelet-rich plasma method, comprising the following steps:
[0050] Step 1: Inject the drawn venous whole blood into the preparation device through a syringe, tilt the injection needle downward at 45 degrees to touch the side hole of the drainage column, and push the injected blood along the side hole of the drainage column through the inner cavity and flow into the red blood cell compartment;
[0051] Step 2: Centrifuge at a speed of 3300 r / min for 5 min to settle the red blood cell components with a specific gravity > 1.08 at the bottom, and then rotate the red blood cell compartment to achieve a red blood cell removal rate of 99.9%.
[0052] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.
[0053] Finally, it should be noted that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.
Claims
1. An efficient platelet-rich plasma preparation device, characterized in that: The highly efficient platelet-rich plasma preparation device comprises a red blood cell chamber (1), a supernatant chamber (2), a platelet-rich plasma chamber (3), a lower chamber cover (5), a blood drainage column (6), a silicone plug (7), a silicone coating layer (8), inner and outer anticoagulant coatings (10) of the drainage column, a sealing ring (11) and a blood outlet (12), wherein the blood drainage column (6) is arranged at the middle position inside the supernatant chamber (2); a drainage column side hole is opened on the upper left side of the blood drainage column (6); the blood drainage column (6) is arranged in a transparent state; and the inner and outer anticoagulant coatings (10) of the drainage column are coated on the outside of the blood drainage column (6).
2. The efficient platelet-rich plasma preparation device according to claim 1, characterized in that: The red blood cell compartment (1), the supernatant compartment (2) and the platelet-rich plasma compartment (3) form three interconnected cavities; the upper part of the supernatant compartment (2) is sleeved with a silicone ring (4); the supernatant compartment (2) is threadedly connected to the upper part of the red blood cell compartment (1); the platelet-rich plasma compartment (3) is located inside the supernatant compartment (2) and is connected to the interior thereof; the lower compartment cover (5) is threadedly connected to the upper end of the platelet-rich plasma compartment (3); and the lower outer part of the platelet-rich plasma compartment (3) is integrally provided with a support area drainage oblique structure.
3. The efficient platelet-rich plasma preparation device according to claim 1, characterized in that: The silicone plug (7) is filled in the upper inner end of the platelet-rich plasma compartment (3); the silicone coating layer (8) is arranged on the lower inner side of the platelet-rich plasma compartment (3); and the upper outer side of the supernatant compartment (2) is threadedly connected to a silicone ring 2 (9).
4. The efficient platelet-rich plasma preparation device according to claim 1, characterized in that: The two end plugs of the blood drainage column (6) are designed with a medical silica gel coating layer (8); the blood outlet (12) is integrated into the lower part of the blood drainage column (6); and the side hole of the drainage column is connected to the inside of the blood outlet (12).
5. The efficient platelet-rich plasma preparation device according to claim 1, characterized in that: The interior of the supernatant chamber (2) has a structure for supporting a blood drainage column, and the supporting structure is designed to have an inclination angle of 5° between the upper and lower contact surfaces; the two ends of the blood drainage column (6) are blocked by a secondary encapsulation injection molding process; and the sealing ring (11) is glued to the intersection between the red blood cell chamber (1), the supernatant chamber (2) and the platelet-rich plasma chamber (3).
6. The efficient platelet-rich plasma preparation device according to claim 1, characterized in that: The device is a blood drainage column, and the inner cavity of the blood drainage column is provided with an anti-coagulation coating to prevent the injected blood from adhering to the wall.
7. A method for producing high-efficiency platelet-rich plasma, characterized in that: The efficient platelet-rich plasma preparation device according to any one of claim 1 is used to process blood products, thereby obtaining efficient platelet-rich plasma.
8. The method according to claim 6, characterized in that The following steps are involved: Step 1: Inject the drawn venous whole blood into the preparation device through a syringe, tilt the injection needle downward to touch the side hole of the drainage column, and push the injected blood along the side hole of the drainage column through the inner cavity and flow into the red blood cell compartment; Step 2: Through secondary centrifugation, the different sedimentation coefficients in the blood are used to separate and precipitate, and the red blood cell components with a specific gravity > 1.08 are settled at the bottom, and then the red blood cell compartment is rotated.
9. The method according to claim 6, characterized in that The centrifugal speed is 3000-3500r / min, and the time is 3-5min. The high-speed re-spin centrifugal method settles the red blood cells in the blood at the bottom of the red blood cell compartment, and the tilt angle is 40-50° downward.
10. The method according to claim 6, characterized in that The red blood cell concentration in PRP should be less than 1X10 6 / UL, red blood cell removal rate greater than 99.9%.