Application of tylosin finished product liquid fertilizer in promoting root development

Abstract

The invention discloses an application of a tylosin finished product liquid fertilizer in promoting root development, and belongs to the technical field of agricultural fertilizers, and the tylosin finished product liquid fertilizer comprises 1-2 parts by mass of a tylosin stock solution; 1.5 to 2.5 parts by mass of polyethylene glycol; 4-8 parts by mass of organic acid; 1-2 parts by mass of a trace element chelating system; 0.8 to 1.8 parts by mass of a biological activity enhancer; 7-9 parts by mass of a nitrogen-phosphorus-potassium source; 0.1-0.3 part by mass of a stabilizer; and 65-75 parts by mass of water. The tylosin finished product liquid fertilizer disclosed by the invention has positive effects in the aspects of promoting plant growth and development, improving product stability, enhancing plant stress resistance and improving crop yield through the unique synergistic effect of all the components.

Description

Technical Field

[0001] The invention relates to the technical field of agricultural fertilizers, and in particular to application of tylosin finished liquid fertilizer in promoting root development. Background Art

[0002] Plant roots are the main organs for plants to absorb water and nutrients. Their development directly affects plant growth, stress resistance and yield. In recent years, with the development of modern agriculture, technical means to promote plant root development have received increasing attention. Liquid fertilizers have been widely used in agricultural production due to their easy absorption and high utilization rate.

[0003] Tylosin is a macrolide antibiotic, and studies have shown that it has a positive effect on plant growth.

[0004] Liquid fertilizers in the prior art generally have the following problems: first, the effective ingredients are single and lack a multi-factor synergistic mechanism, resulting in limited root promotion effects; second, the product stability is poor and the effective period is short; third, the effect is insufficient in improving plant stress resistance and enhancing plant biomass accumulation; fourth, it is impossible to simultaneously take into account the dual goals of promoting root development and increasing yield. Summary of the invention

[0005] The invention provides an application of tylosin finished liquid fertilizer to promote root development, which solves the technical problems in the prior art of single effective ingredient of liquid fertilizer, poor product stability and inability to simultaneously take into account root development promotion and yield improvement.

[0006] The invention discloses a tylosin finished liquid fertilizer for promoting plant root development, comprising: 1-2 parts by mass of tylosin stock solution; 1.5-2.5 parts by mass of polyethylene glycol; 4-8 parts by weight of organic acid; 1-2 parts by mass of trace element chelating system; 0.8-1.8 parts by weight of biological activity enhancer; 7-9 parts by weight of nitrogen, phosphorus and potassium sources; Stabilizer 0.1-0.3 parts by mass; Water content: 65-75 parts by mass.

[0007] Preferably, the organic acid is composed of citric acid, ferulic acid and sodium humate, wherein the citric acid is 4-7 parts by mass, the ferulic acid is 0.5-1 parts by mass, and the sodium humate is 1-2 parts by mass.

[0008] Preferably, the trace element chelating system comprises: 0.7-1 parts by weight of disodium EDTA; Zinc sulfate 0.4-0.8 parts by mass; 0.3-0.6 parts by mass of iron sulfate; Copper sulfate 0.1-0.2 parts by mass; Manganese sulfate 0.2-0.4 parts by mass.

[0009] Preferably, the bioactivity enhancer comprises: 0.3-0.5 parts by weight of seaweed extract; Betaine 0.2-0.5 parts by mass; Chitosan 0.15-0.35 parts by mass; 0.05-0.15 parts by mass of 5-aminolevulinic acid.

[0010] Preferably, the nitrogen, phosphorus and potassium source comprises: Urea 2.5-4.5 parts by mass; 2-3.5 parts by mass of diammonium phosphate; Potassium sulfate 1.8-2.5 parts by mass.

[0011] Preferably, the stabilizer consists of 0.15-0.25 parts by mass of potassium sorbate and 0.1-0.2 parts by mass of polyacrylamide.

[0012] The present invention also provides a method for preparing the liquid fertilizer, comprising the following steps: (1) Tylosin activation pretreatment: deionized water is heated to 35-38° C., an organic acid is added, and polyethylene glycol is added after stirring to dissolve, and stirring is continued until completely dissolved, followed by adding tylosin stock solution, stirring for 90-120 minutes in a dark environment, and finally glycerol is added as a stabilizer, and mixed evenly to obtain a tylosin activation solution; (2) adding deionized water to a reaction kettle, adding disodium EDTA and stirring to dissolve, adding zinc sulfate, iron sulfate, copper sulfate and manganese sulfate in sequence, adjusting the temperature to 40-45° C., stirring for 60 minutes to form a metal chelate solution, then adding ferulic acid and sodium humate, continuing to stir for 90 minutes, adjusting the pH value to 5.8-6.2, and obtaining a nutrient element complex solution; (3) Preparation of bioactivity enhancer: adding seaweed extract to deionized water at 40-45°C, stirring for 20 minutes using a high shear emulsifier, adding betaine and stirring until completely dissolved, adding chitosan to the aqueous solution containing lactic acid and stirring until transparent, mixing the two solutions, adding 5-aminolevulinic acid and potassium dihydrogen phosphate, stirring for 60 minutes at 45-50°C, and cooling to room temperature to obtain a bioactivity enhancer; (4) Add the tylosin activation solution of step (1) into the reactor, slowly add the nutrient element complex solution of step (2) and stir for 30 minutes, add the biological activity enhancer of step (3) and continue stirring for 60 minutes, add urea, ammonium dihydrogen phosphate and potassium sulfate in proportion and stir until completely dissolved, add potassium sorbate and polyacrylamide and stir for 90 minutes, and finally filter to remove insoluble matter to obtain a clear liquid product.

[0013] Preferably, the organic acid in step (1) is citric acid, and the stabilizer is glycerol; the lactic acid concentration in step (3) is 70-85%, and the filtration in step (4) uses a filter with a pore size of 0.45 μm.

[0014] Preferably, the chitosan has a deacetylation degree of 80-90% and a molecular weight of 80-120 kDa; the molecular weight of the polyethylene glycol is 3800-4200; and the molecular weight of the polyacrylamide is 5-8 million Da.

[0015] The invention also provides an application of the liquid fertilizer in promoting the development of plant root systems.

[0016] Compared with the prior art, the present invention has at least the following beneficial effects: (1) The present invention significantly promotes the development of plant roots through the synergistic effect of tylosin, multiple organic acids and bioactive enhancers in a specific formula. After using the liquid fertilizer, the height of cucumber plants can reach 82.6 cm, which is 41.4% higher than that of the control group; the stem thickness can reach 13.0 mm.

[0017] (2) The composite system formed by the bioactive enhancer and tylosin in the formula of the present invention improves the stress resistance and biomass accumulation of the plant. The biomass of the cucumber plants treated with the liquid fertilizer of the present invention can reach 298.3 g / plant, which is 52.5% higher than that of the control group.

[0018] (3) The liquid fertilizer of the present invention significantly increases crop yield by improving the development and growth of plant roots. Field test data show that the yield of cucumbers treated with the liquid fertilizer can reach 2.96 kg / plant.

[0019] In summary, the tylosin finished liquid fertilizer of the present invention has positive effects in promoting plant growth and development, improving product stability, enhancing plant stress resistance and increasing crop yield through the unique synergistic effect of various components. DETAILED DESCRIPTION

[0020] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0021] The polyethylene glycol used in the examples of the present application was purchased from BASF, Germany, with an average molecular weight of 6000Da and a purity of ≥99%; the tylosin stock solution used was from Shandong Lukang Pharmaceutical Co., Ltd., with a product specification of 8% aqueous solution and a batch number of SL20230526; the chitosan used was from Qingdao Mingyue Seaweed Group Co., Ltd., with a product batch number of CH-2023-078, a deacetylation degree of 85±2%, a molecular weight of 100±5kDa, and a purity of ≥95%; polyacrylamide was from the French Aisen Group, with a purity of ≥98% and a molecular weight of 6.5 million Da; the filter with a pore size of 0.45 μm was from Merck Millipore, model Stericup™ GP, which is a polyethersulfone (PES) membrane filtration system.

[0022] Example 1 S1. Heat 12 kg of deionized water to 36°C, add 5 kg of citric acid, stir to dissolve, then add 2 kg of polyethylene glycol, continue stirring until completely dissolved, then add 1.5 kg of tylosin stock solution, stir for 105 minutes in a dark environment, finally add 0.2 kg of glycerol as a stabilizer, mix well, and obtain tylosin activated solution.

[0023] S2. Add 15 kg of deionized water to the reactor, add 0.8 kg of disodium EDTA and stir to dissolve, add 0.6 kg of zinc sulfate, 0.4 kg of iron sulfate, 0.15 kg of copper sulfate and 0.3 kg of manganese sulfate in sequence, adjust the temperature to 42 ° C, stir for 60 minutes to form a metal chelate solution, then add 0.8 kg of ferulic acid and 1.5 kg of sodium humate, continue stirring for 90 minutes, adjust the pH value to 6.0, and obtain a nutrient element complex solution.

[0024] S3. At 42°C, add 0.4 kg of seaweed extract to 8 kg of deionized water, stir using a high shear emulsifier for 20 minutes, add 0.4 kg of betaine and stir until completely dissolved, add 0.25 kg of chitosan to an aqueous solution containing 80% lactic acid and stir until transparent, mix the two solutions, add 0.1 kg of 5-aminolevulinic acid and 2.5 kg of potassium dihydrogen phosphate, stir at 48°C for 60 minutes, cool to room temperature, and obtain a bioactivity enhancer.

[0025] S4. Add tylosin activation solution into the reactor, slowly add the nutrient element complex solution and stir for 30 minutes, add the biological activity enhancer and continue stirring for 60 minutes, add 3.5 kg of urea, 3 kg of diammonium phosphate and 2 kg of potassium sulfate in proportion and stir until completely dissolved, add 0.2 kg of potassium sorbate and 0.15 kg of polyacrylamide and stir for 90 minutes, and finally use a filter with a pore size of 0.45 μm to filter out insoluble matter to obtain a clear liquid product, which is the tylosin finished liquid fertilizer.

[0026] Example 2 S1. Heat 13 kg of deionized water to 37°C, add 6 kg of citric acid, stir to dissolve, then add 1.8 kg of polyethylene glycol, continue stirring until completely dissolved, then add 1.2 kg of tylosin stock solution, stir for 100 minutes in a dark environment, finally add 0.25 kg of glycerol as a stabilizer, mix well, and obtain tylosin activated solution.

[0027] S2. Add 14 kg of deionized water to the reactor, add 0.9 kg of disodium EDTA and stir to dissolve, add 0.7 kg of zinc sulfate, 0.5 kg of iron sulfate, 0.18 kg of copper sulfate and 0.35 kg of manganese sulfate in sequence, adjust the temperature to 44 ° C, stir for 60 minutes to form a metal chelate solution, then add 0.9 kg of ferulic acid and 1.8 kg of sodium humate, continue stirring for 90 minutes, adjust the pH value to 6.1, and obtain a nutrient element complex solution.

[0028] S3. At 43°C, add 0.45 kg of seaweed extract to 9 kg of deionized water, stir using a high shear emulsifier for 20 minutes, add 0.35 kg of betaine and stir until completely dissolved, add 0.3 kg of chitosan to an aqueous solution containing 75% lactic acid and stir until transparent, mix the two solutions, add 0.12 kg of 5-aminolevulinic acid and 2.8 kg of potassium dihydrogen phosphate, stir at 47°C for 60 minutes, cool to room temperature, and obtain a bioactivity enhancer.

[0029] S4. Add tylosin activation solution into the reactor, slowly add the nutrient element complex solution and stir for 30 minutes, add the biological activity enhancer and continue stirring for 60 minutes, add 4 kg of urea, 2.8 kg of ammonium dihydrogen phosphate and 2.3 kg of potassium sulfate in proportion and stir until completely dissolved, add 0.22 kg of potassium sorbate and 0.18 kg of polyacrylamide and stir for 90 minutes, finally use a filter with a pore size of 0.45 μm to filter out insoluble matter to obtain a clear liquid product, which is the tylosin finished liquid fertilizer.

[0030] Example 3 S1. Heat 14 kg of deionized water to 35°C, add 4.5 kg of citric acid, stir to dissolve, then add 2.2 kg of polyethylene glycol, continue stirring until completely dissolved, then add 1.8 kg of tylosin stock solution, stir for 120 minutes in a dark environment, finally add 0.18 kg of glycerol as a stabilizer, mix well, and obtain tylosin activated solution.

[0031] S2. Add 16 kg of deionized water to the reactor, add 0.75 kg of disodium EDTA and stir to dissolve, add 0.5 kg of zinc sulfate, 0.35 kg of iron sulfate, 0.12 kg of copper sulfate and 0.25 kg of manganese sulfate in sequence, adjust the temperature to 40°C, stir for 60 minutes to form a metal chelate solution, then add 0.7 kg of ferulic acid and 1.2 kg of sodium humate, continue stirring for 90 minutes, adjust the pH value to 5.9, and obtain a nutrient element complex solution.

[0032] S3. At 45°C, add 0.35 kg of seaweed extract to 8.5 kg of deionized water, stir for 20 minutes using a high shear emulsifier, add 0.45 kg of betaine and stir until completely dissolved, add 0.2 kg of chitosan to an aqueous solution containing 85% lactic acid and stir until transparent, mix the two solutions, add 0.08 kg of 5-aminolevulinic acid and 2.2 kg of potassium dihydrogen phosphate, stir at 50°C for 60 minutes, cool to room temperature, and obtain a bioactivity enhancer.

[0033] S4. Add tylosin activation solution into the reactor, slowly add the nutrient element complex solution and stir for 30 minutes, add the biological activity enhancer and continue stirring for 60 minutes, add 3 kg of urea, 3.2 kg of ammonium dihydrogen phosphate and 1.9 kg of potassium sulfate in proportion and stir until completely dissolved, add 0.18 kg of potassium sorbate and 0.12 kg of polyacrylamide and stir for 90 minutes, finally use a filter with a pore size of 0.45 μm to filter out insoluble matter to obtain a clear liquid product, which is the tylosin finished liquid fertilizer.

[0034] Example 4 S1. Heat 13.5 kg of deionized water to 38°C, add 7 kg of citric acid, stir to dissolve, then add 2.4 kg of polyethylene glycol, continue stirring until completely dissolved, then add 2 kg of tylosin stock solution, stir for 110 minutes in a dark environment, finally add 0.22 kg of glycerol as a stabilizer, mix well, and obtain tylosin activated solution.

[0035] S2. Add 15.5 kg of deionized water to a reactor, add 1 kg of disodium EDTA and stir to dissolve, add 0.8 kg of zinc sulfate, 0.6 kg of iron sulfate, 0.2 kg of copper sulfate and 0.4 kg of manganese sulfate in sequence, adjust the temperature to 45°C, stir for 60 minutes to form a metal chelate solution, then add 1 kg of ferulic acid and 2 kg of sodium humate, continue stirring for 90 minutes, adjust the pH value to 6.2, and obtain a nutrient element complex solution.

[0036] S3. At 44°C, add 0.5 kg of seaweed extract to 9.5 kg of deionized water, stir for 20 minutes using a high shear emulsifier, add 0.5 kg of betaine and stir until completely dissolved, add 0.35 kg of chitosan to an aqueous solution containing 70% lactic acid and stir until transparent, mix the two solutions, add 0.15 kg of 5-aminolevulinic acid and 3 kg of potassium dihydrogen phosphate, stir at 46°C for 60 minutes, cool to room temperature, and obtain a bioactivity enhancer.

[0037] S4. Add tylosin activation solution into the reactor, slowly add the nutrient element complex solution and stir for 30 minutes, add the biological activity enhancer and continue stirring for 60 minutes, add 4.5 kg of urea, 3.5 kg of ammonium dihydrogen phosphate and 2.5 kg of potassium sulfate in proportion and stir until completely dissolved, add 0.25 kg of potassium sorbate and 0.2 kg of polyacrylamide and stir for 90 minutes, finally use a filter with a pore size of 0.45 μm to filter out insoluble matter to obtain a clarified liquid product, which is the tylosin finished liquid fertilizer.

[0038] Example 5 S1. Heat 12.5 kg of deionized water to 35.5°C, add 4 kg of citric acid, stir to dissolve, then add 1.5 kg of polyethylene glycol, continue stirring until completely dissolved, then add 1 kg of tylosin stock solution, stir for 90 minutes in a dark environment, finally add 0.15 kg of glycerol as a stabilizer, mix well, and obtain tylosin activated solution.

[0039] S2. Add 14.5 kg of deionized water to the reactor, add 0.7 kg of disodium EDTA and stir to dissolve, add 0.4 kg of zinc sulfate, 0.3 kg of iron sulfate, 0.1 kg of copper sulfate and 0.2 kg of manganese sulfate in sequence, adjust the temperature to 40°C, stir for 60 minutes to form a metal chelate solution, then add 0.5 kg of ferulic acid and 1 kg of sodium humate, continue stirring for 90 minutes, adjust the pH value to 5.8, and obtain a nutrient element complex solution.

[0040] S3. At 40°C, add 0.3 kg of seaweed extract to 7.5 kg of deionized water, stir using a high shear emulsifier for 20 minutes, add 0.2 kg of betaine and stir until completely dissolved, add 0.15 kg of chitosan to an aqueous solution containing 75% lactic acid and stir until transparent, mix the two solutions, add 0.05 kg of 5-aminolevulinic acid and 2 kg of potassium dihydrogen phosphate, stir at 45°C for 60 minutes, cool to room temperature, and obtain a bioactivity enhancer.

[0041] S4. Add tylosin activation solution into the reactor, slowly add the nutrient element complex solution and stir for 30 minutes, add the biological activity enhancer and continue stirring for 60 minutes, add 2.5 kg of urea, 2 kg of ammonium dihydrogen phosphate and 1.8 kg of potassium sulfate in proportion and stir until completely dissolved, add 0.15 kg of potassium sorbate and 0.1 kg of polyacrylamide and stir for 90 minutes, finally use a filter with a pore size of 0.45 μm to filter out insoluble matter to obtain a clear liquid product, which is the tylosin finished liquid fertilizer.

[0042] Comparative Example 1 S1. Heat 12 kg of deionized water to 36°C, add 5 kg of citric acid, stir to dissolve, then add 2 kg of polyethylene glycol, continue stirring until completely dissolved, add 1.5 kg of distilled water, stir for 105 minutes, and finally add 0.2 kg of glycerol as a stabilizer, mix well, and obtain a contrast activation solution.

[0043] S2. Add 15 kg of deionized water to the reactor, add 0.8 kg of disodium EDTA and stir to dissolve, add 0.6 kg of zinc sulfate, 0.4 kg of iron sulfate, 0.15 kg of copper sulfate and 0.3 kg of manganese sulfate in sequence, adjust the temperature to 42 ° C, stir for 60 minutes to form a metal chelate solution, then add 0.8 kg of ferulic acid and 1.5 kg of sodium humate, continue stirring for 90 minutes, adjust the pH value to 6.0, and obtain a nutrient element complex solution.

[0044] S3. At 42°C, add 0.4 kg of seaweed extract to 8 kg of deionized water, stir using a high shear emulsifier for 20 minutes, add 0.4 kg of betaine and stir until completely dissolved, add 0.25 kg of chitosan to an aqueous solution containing 80% lactic acid and stir until transparent, mix the two solutions, add 0.1 kg of 5-aminolevulinic acid and 2.5 kg of potassium dihydrogen phosphate, stir at 48°C for 60 minutes, cool to room temperature, and obtain a bioactivity enhancer.

[0045] S4. Add the contrast activation liquid into the reactor, slowly add the nutrient element complex liquid and stir for 30 minutes, add the biological activity enhancer and continue stirring for 60 minutes, add 3.5 kg of urea, 3 kg of diammonium phosphate and 2 kg of potassium sulfate in proportion and stir until completely dissolved, add 0.2 kg of potassium sorbate and 0.15 kg of polyacrylamide and stir for 90 minutes, finally use a filter with a pore size of 0.45 μm to filter out insoluble matter to obtain a clear liquid product, which is liquid fertilizer.

[0046] Comparative Example 2 S1. Heat 12 kg of deionized water to 36°C, add 5 kg of citric acid, stir to dissolve, then add 2 kg of polyethylene glycol, continue stirring until completely dissolved, then add 1.5 kg of tylosin stock solution, stir for 105 minutes in a dark environment, finally add 0.2 kg of glycerol as a stabilizer, mix well, and obtain tylosin activated solution.

[0047] S2. Add 15 kg of deionized water to the reactor, add 0.8 kg of disodium EDTA and stir to dissolve, add 0.6 kg of zinc sulfate, 0.4 kg of iron sulfate, 0.15 kg of copper sulfate and 0.3 kg of manganese sulfate in turn, adjust the temperature to 42°C, stir for 60 minutes to form a metal chelate solution, add 2.3 kg of distilled water, adjust the pH value to 6.0, and obtain a nutrient element complex solution. S3. Add 0.4 kg of seaweed extract to 8 kg of deionized water at 42°C, stir for 20 minutes using a high shear emulsifier, add 0.4 kg of betaine and stir until completely dissolved, add 0.25 kg of chitosan to an aqueous solution containing 80% lactic acid and stir until transparent, mix the two solutions, add 0.1 kg of 5-aminolevulinic acid and 2.5 kg of potassium dihydrogen phosphate, stir for 60 minutes at 48°C, cool to room temperature, and obtain a bioactivity enhancer.

[0048] S4. Add tylosin activation solution into the reactor, slowly add the nutrient element complex solution and stir for 30 minutes, add the biological activity enhancer and continue stirring for 60 minutes, add 3.5 kg of urea, 3 kg of ammonium dihydrogen phosphate and 2 kg of potassium sulfate in proportion and stir until completely dissolved, add 0.2 kg of potassium sorbate and 0.15 kg of polyacrylamide and stir for 90 minutes, finally use a filter with a pore size of 0.45 μm to filter out insoluble matter to obtain a clear liquid product, i.e., liquid fertilizer.

[0049] Comparative Example 3 S1. Heat 12 kg of deionized water to 36°C, add 5 kg of citric acid, stir to dissolve, then add 2 kg of polyethylene glycol, continue stirring until completely dissolved, then add 1.5 kg of tylosin stock solution, stir for 105 minutes in a dark environment, add 0.2 kg of glycerol as a stabilizer, mix well, and obtain tylosin activated solution.

[0050] S2. Add 15 kg of deionized water to the reactor, add 0.8 kg of disodium EDTA and stir to dissolve, add 0.6 kg of zinc sulfate, 0.4 kg of iron sulfate, 0.15 kg of copper sulfate and 0.3 kg of manganese sulfate in sequence, adjust the temperature to 42 ° C, stir for 60 minutes to form a metal chelate solution, then add 0.8 kg of ferulic acid and 1.5 kg of sodium humate, continue stirring for 90 minutes, adjust the pH value to 6.0, and obtain a nutrient element complex solution.

[0051] S3. At 42° C., add 2.5 kg of potassium dihydrogen phosphate to 8.5 kg of deionized water, stir at 48° C. for 60 minutes, and cool to room temperature to obtain a contrast enhancing agent.

[0052] S4. Add tylosin activation solution into the reactor, slowly add the nutrient element complex solution and stir for 30 minutes, add contrast enhancer and continue stirring for 60 minutes, add 3.5 kg of urea, 3 kg of diammonium phosphate and 2 kg of potassium sulfate in proportion and stir until completely dissolved, add 0.2 kg of potassium sorbate and 0.15 kg of polyacrylamide and stir for 90 minutes, finally use a filter with a pore size of 0.45 μm to filter out insoluble matter to obtain a clear liquid product, i.e., liquid fertilizer.

[0053] Performance Testing Detection Methods Eight groups were set up, and each group selected liquid fertilizers of different embodiments and comparative examples as samples for performance testing.

[0054] 1. Root growth promotion effect test Cucumber seeds were selected as test materials. After the seeds were sterilized, they were pre-germinated on moist filter paper. The seeds with neat germination were selected and transplanted into a culture pot filled with nutrient soil. When the seedlings grew two true leaves, the liquid fertilizer of each group was diluted at a ratio of 1:500 and watered on the roots of the seedlings, with 50 ml per pot. Ten pots were treated in each group, with 3 plants in each pot. After 14 days of treatment, the plant roots were washed and the taproot length, the number of lateral roots and the fresh weight of the roots were measured.

[0055] 2. Stress resistance test Two-week-old cucumber seedlings were treated with liquid fertilizers of each group diluted at 1:500 for 7 days and then placed in a high temperature environment of 35°C for 48 hours. The relative water content of the leaves, malondialdehyde content and superoxide dismutase activity were measured.

[0056] 3. Product stability test Each group of liquid fertilizers was placed in an environment of 25°C for 90 consecutive days, and the pH value, effective content of tylosin and decomposition rate of the active ingredient were measured every 30 days to evaluate the long-term stability of the product.

[0057] 4. Field application effect evaluation The experiment was conducted under the conditions of cucumber field production. Each treatment group was diluted 1:500 and applied once every 7 days for 4 consecutive times. The plant height, stem diameter, leaf area index, biomass and yield data were recorded 30 days after treatment.

[0058] Test Results Table 1 Root growth promotion effect test record Treatment Group Taproot length (cm) Number of lateral roots (roots / plant) Fresh root weight (g / plant) Root dry weight (g / plant) Example 1 15.8 78.3 4.26 0.45 Example 2 16.2 80.5 4.32 0.47 Example 3 15.5 76.8 4.18 0.44 Example 4 16.8 82.1 4.45 0.49 Example 5 15.1 75.2 4.10 0.43 Comparative Example 1 10.3 48.5 2.65 0.28 Comparative Example 2 12.4 59.6 3.21 0.34 Comparative Example 3 11.8 55.2 3.05 0.32 Clear water control 8.6 41.3 2.28 0.24 Table 2 Stress resistance test result record Treatment Group Relative water content of leaves (%) MDA content (μmol / g) SOD activity (U / g·FW) Chlorophyll content (mg / g) Example 1 82.4 4.8 152.6 2.85 Example 2 83.1 4.5 156.3 2.92 Example 3 81.8 5.1 150.4 2.81 Example 4 84.5 4.2 159.5 3.05 Example 5 80.6 5.3 148.2 2.78 Comparative Example 1 68.3 8.7 108.4 2.14 Comparative Example 2 72.5 7.5 115.6 2.32 Comparative Example 3 70.8 7.9 112.3 2.25 Clear water control 62.4 10.2 95.7 1.95 Table 3 Product stability test record Treatment Group Initial pH pH value after 90 days Tylosin active ingredient retention rate Decomposition rate of active ingredients Example 1 6.2 6.0 94.2% 5.8% Example 2 6.3 6.1 93.8% 6.2% Example 3 6.1 5.9 95.3% 4.7% Example 4 6.4 6.2 92.5% 7.5% Example 5 6.0 5.8 96.1% 3.9% Comparative Example 1 - - - - Comparative Example 2 6.2 5.4 68.3% 31.7% Comparative Example 3 6.1 5.5 71.5% 28.5% Table 4 Field application record Treatment Group Plant height(cm) Stem diameter (mm) Leaf Area Index Biomass (g / plant) Yield (kg / plant) Example 1 78.5 12.3 4.85 285.6 2.82 Example 2 80.2 12.6 4.92 291.4 2.88 Example 3 77.3 12.1 4.76 280.5 2.75 Example 4 82.6 13.0 5.05 298.3 2.96 Example 5 76.1 11.9 4.68 275.2 2.70 Comparative Example 1 65.3 9.6 3.52 218.4 2.05 Comparative Example 2 68.7 10.2 3.78 232.1 2.18 Comparative Example 3 67.5 10.0 3.65 225.8 2.12 Clear water control 58.4 8.5 3.14 195.6 1.83 Data analysis As can be seen from Table 1, Examples 1-5 of the present invention have a significant promoting effect on the growth of cucumber roots, among which Example 4 has the best effect, with the taproot length reaching 16.8 cm, 63.1% higher than Comparative Example 1 and 95.3% higher than the clear water control; the number of lateral roots reaches 82.1 per plant, 69.3% higher than Comparative Example 1 and 98.8% higher than the clear water control; the fresh weight of the root system reaches 4.45 g per plant, 67.9% higher than Comparative Example 1 and 95.2% higher than the clear water control. The root development of Comparative Example 1 lacking tylosin stock solution is obviously restricted, indicating that tylosin is a key active ingredient in promoting root development. Comparative Example 2 lacking organic acid and Comparative Example 3 lacking bioactive enhancer also show weak root promotion effects, which are 25.3% and 21.5% lower than Example 1, respectively. These data show that tylosin, organic acid and bioactive enhancer work synergistically to promote plant root development.

[0059] As can be seen from Table 2, the cucumber plants treated with Examples 1-5 showed stronger stress resistance under high temperature stress. Example 4 performed best, with a relative water content of 84.5% in its leaves, 23.7% higher than that of Comparative Example 1 and 35.4% higher than that of the clear water control; the MDA content was only 4.2 μmol / g, 51.7% lower than that of Comparative Example 1 and 58.8% lower than that of the clear water control; the SOD activity reached 159.5 U / g·FW, 47.1% higher than that of Comparative Example 1 and 66.7% higher than that of the clear water control; the chlorophyll content reached 3.05 mg / g, 42.5% higher than that of Comparative Example 1 and 56.4% higher than that of the clear water control. The stress resistance of Comparative Examples 1-3 was significantly lower than that of the Example group, especially in the absence of tylosin, the plant stress resistance of Comparative Example 1 was the weakest. This shows that tylosin significantly improves the ability of plants to obtain water and nutrients from the soil by enhancing root development, thereby improving the resistance of plants to environmental stress. At the same time, the absence of organic acids and bioactive enhancers also significantly reduced the stress resistance of plants, indicating that there is a synergistic effect between these components and tylosin.

[0060] As can be seen from Table 3, after the liquid fertilizer of Embodiment 1-5 is stored for 90 days at room temperature, the pH value changes little, only decreases by about 0.2 unit, the retention rate of tylosin effective ingredients is between 92.5% and 96.1%, and the decomposition rate of effective ingredients is lower than 8%. Wherein Embodiment 5 performs best, the retention rate of effective ingredients reaches 96.1%, and the decomposition rate is only 3.9%. And the pH value of Comparative Example 2 lacking organic acid system and Comparative Example 3 lacking biological activity enhancer decreases greatly, respectively reduces by 0.8 and 0.6 unit, the retention rate of tylosin effective ingredients is obviously lower than the embodiment group, only 68.3% and 71.5%, and the corresponding decomposition rate is up to 31.7% and 28.5%. This shows that the organic acid system and biological activity enhancer of the present invention can not only promote the development of plant root system, but also stabilize the molecular structure of tylosin, slow down its degradation rate in solution, and significantly improve the storage stability of product.

[0061] As can be seen from Table 4, when applied to cucumber field production, Examples 1-5 significantly promoted the growth of cucumber plants and increased yield. Example 4 performed best, with a cucumber plant of 82.6 cm after treatment, 26.5% higher than Comparative Example 1 and 41.4% higher than the clear water control; the stem diameter reached 13.0 mm, 35.4% higher than Comparative Example 1 and 52.9% higher than the clear water control; the leaf area index reached 5.05, 43.5% higher than Comparative Example 1 and 60.8% higher than the clear water control; the biomass reached 298.3 g / plant, 36.6% higher than Comparative Example 1 and 52.5% higher than the clear water control; the yield reached 2.96 kg / plant, 44.4% higher than Comparative Example 1 and 61.7% higher than the clear water control. The treatment effect of Comparative Examples 1-3 was significantly lower than that of the embodiment group, among which the effect of Comparative Example 1 without tylosin was the weakest, indicating that tylosin is the core active ingredient of the liquid fertilizer of the present invention in promoting plant growth. The effects of Comparative Example 2 lacking organic acid and Comparative Example 3 lacking biological activity enhancer are also significantly weaker than those of the Example group, indicating that the organic acid system and biological activity enhancer play an important role in improving the bioavailability of tylosin and promoting plant growth. These field test data fully prove that the tylosin finished liquid fertilizer of the present invention significantly enhances the growth potential and yield performance of plants by promoting root development.

[0062] In summary, the tylosin finished liquid fertilizer of the present invention shows significant effects in promoting plant root development, improving plant stress resistance, maintaining product stability and promoting crop yield. In contrast, comparative example 1 lacking tylosin, comparative example 2 lacking organic acid and comparative example 3 lacking biological activity enhancer all show obvious efficacy defects, proving the synergistic effect between the components of the present invention.

[0063] Although the embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that all equivalent changes and improvements made within the scope of the present invention should still fall within the scope of the patent coverage of the present invention.

Claims

1. A tylosin finished liquid fertilizer for promoting plant root development, characterized in that: include: 1-2 parts by mass of tylosin stock solution; 1.5-2.5 parts by mass of polyethylene glycol; 4-8 parts by weight of organic acid; 1-2 parts by mass of trace element chelating system; 0.8-1.8 parts by weight of biological activity enhancer; 7-9 parts by weight of nitrogen, phosphorus and potassium source; Stabilizer 0.1-0.3 parts by mass; Water content: 65-75 parts by mass.

2. Liquid fertilizer according to claim 1, characterized in that, The organic acid consists of citric acid, ferulic acid and sodium humate, wherein the citric acid is 4-7 parts by weight, the ferulic acid is 0.5-1 parts by weight and the sodium humate is 1-2 parts by weight.

3. Liquid fertilizer according to claim 1, characterized in that, The trace element chelating system comprises: 0.7-1 parts by weight of disodium EDTA; Zinc sulfate 0.4-0.8 parts by mass; 0.3-0.6 parts by mass of iron sulfate; Copper sulfate 0.1-0.2 parts by mass; Manganese sulfate 0.2-0.4 parts by mass.

4. Liquid fertilizer according to claim 1, characterized in that, The bioactivity enhancer includes: 0.3-0.5 parts by weight of seaweed extract; Betaine 0.2-0.5 parts by mass; Chitosan 0.15-0.35 parts by mass; 0.05-0.15 parts by mass of 5-aminolevulinic acid.

5. Liquid fertilizer according to claim 1, characterized in that, The nitrogen, phosphorus and potassium source comprises: Urea 2.5-4.5 parts by mass; 2-3.5 parts by mass of diammonium phosphate; Potassium sulfate 1.8-2.5 parts by mass.

6. Liquid fertilizer according to claim 1, characterized in that, The stabilizer consists of 0.15-0.25 parts by mass of potassium sorbate and 0.1-0.2 parts by mass of polyacrylamide.

7. A method for preparing a liquid fertilizer as claimed in any one of claims 1 to 6, characterized in that: The following steps are involved: (1) Tylosin activation pretreatment: deionized water is heated to 35-38° C., an organic acid is added, and polyethylene glycol is added after stirring to dissolve, and stirring is continued until completely dissolved, followed by adding tylosin stock solution, stirring for 90-120 minutes in a dark environment, and finally glycerol is added as a stabilizer, and mixed evenly to obtain a tylosin activation solution; (2) adding deionized water to a reaction kettle, adding disodium EDTA and stirring to dissolve, adding zinc sulfate, iron sulfate, copper sulfate and manganese sulfate in sequence, adjusting the temperature to 40-45° C., stirring for 60 minutes to form a metal chelate solution, then adding ferulic acid and sodium humate, continuing to stir for 90 minutes, adjusting the pH value to 5.8-6.2, and obtaining a nutrient element complex solution; (3) Preparation of bioactivity enhancer: adding seaweed extract to deionized water at 40-45°C, stirring for 20 minutes using a high shear emulsifier, adding betaine and stirring until completely dissolved, adding chitosan to the aqueous solution containing lactic acid and stirring until transparent, mixing the two solutions, adding 5-aminolevulinic acid and potassium dihydrogen phosphate, stirring for 60 minutes at 45-50°C, and cooling to room temperature to obtain a bioactivity enhancer; (4) Add the tylosin activation solution of step (1) into the reactor, slowly add the nutrient element complex solution of step (2) and stir for 30 minutes, add the biological activity enhancer of step (3) and continue stirring for 60 minutes, add urea, ammonium dihydrogen phosphate and potassium sulfate in proportion and stir until completely dissolved, add potassium sorbate and polyacrylamide and stir for 90 minutes, and finally filter to remove insoluble matter to obtain a clear liquid product.

8. The preparation method according to claim 7, characterized in that: The organic acid in step (1) is citric acid, and the stabilizer is glycerol; the lactic acid concentration in step (3) is 70-85%, and the filtration in step (4) uses a filter with a pore size of 0.45 μm.

9. The method according to claim 8, characterized in that The deacetylation degree of the chitosan is 80-90%, and the molecular weight is 80-120 kDa; the molecular weight of the polyethylene glycol is 3800-4200; and the molecular weight of the polyacrylamide is 5-8 million Da.

10. Use of the liquid fertilizer according to any one of claims 1 to 7 in promoting the development of plant roots.