Full-automatic single-molecule fluorescence immunoassay analyzer

Abstract

The invention relates to the technical field of immunoassay, in particular to a full-automatic single-molecule fluorescence immunoassay analyzer which comprises an analyzer body and a sample adding bin and further comprises a plurality of transfer pipes located in the sample adding bin. The knocking rods are located in the sample adding bin, the knocking rods are used for knocking and vibrating the bottom of the transfer pipe, when the full-automatic single-molecule fluorescence immunoassay analyzer is used, the existence of bubbles can interfere with reading of fluorescence signals, so that an analysis result is inaccurate, the bubbles are discharged through knocking and vibrating, and the accuracy of the analysis result is improved. According to the method, accurate reading of fluorescence signals can be ensured, so that the analysis accuracy is improved, the fluorescence signal intensity is optimized, bubbles can shield part of the fluorescence signals, the signal intensity is weakened, after the bubbles are discharged, the fluorescence signals can be more fully detected, the signal intensity is optimized, and the result is clearer.

Description

Technical Field

[0001] The invention relates to the technical field of immunoassay analysis, in particular to a full-automatic single-molecule fluorescence immunoassay analyzer. Background Art

[0002] The fully automatic single-molecule fluorescence immunoassay analyzer is a highly sensitive analytical device that integrates immunological principles and advanced fluorescence labeling technology. Its core function is to utilize the specific binding reaction between antigens and antibodies and use precise fluorescent substances as labeling signals to achieve accurate tracking and quantitative detection of biological molecules. This instrument can not only greatly improve the sensitivity of detection to the single-molecule level, but also ensure the accuracy and reliability of the analysis, providing strong support for multiple research fields such as neuroscience, oncology, and infectious diseases.

[0003] In terms of operation process, fluorescent immunoassay usually involves multiple steps such as sample pretreatment, labeling and binding of specific antibodies, and detection and analysis of fluorescent signals. First, the processed sample is poured into the analysis cup, and then an appropriate amount of fluorescent labeled antibody is injected into it and mixed evenly to ensure that the antibody can evenly penetrate and cover the sample. After mixing, the sample is dripped into the sample card vertically using a pipette. During this process, bubbles must be avoided to ensure that the sample is evenly distributed in the card. Existing test card sample addition usually relies on manual sample addition. If the pipette tip and the pipette are not in close contact during the manual pipetting process, the liquid may be sucked up. Air is introduced in the process to form bubbles. At the same time, the working principle of the pipette is usually to absorb and discharge the liquid through the movement of the air cushion or the piston. If the movement speed of the piston is too fast or unstable during use, bubbles may also be generated. The bubbles will block the fluorescent signal, resulting in some areas being unable to be fully stained or marked by fluorescent markers, which will cause the fluorescent signal to be intermittent, weakened or uneven, thereby affecting the detection and reading of the fluorescent signal. In fluorescent immunoassay, the intensity and distribution of the fluorescent signal are important bases for judging the experimental results. Therefore, the presence of bubbles will interfere with this judgment process. For this reason, we propose a fully automatic single-molecule fluorescent immunoassay instrument. Summary of the invention

[0004] One of the technical problems to be solved by the present application is that manual sample addition may result in the generation of bubbles, which may block the fluorescent signal and cause some areas to be unable to be adequately stained or marked by the fluorescent marker, which may cause the fluorescent signal to be intermittent, weakened or uneven, thereby affecting the detection and reading of the fluorescent signal. To solve the above technical problems, the embodiment of the present application provides a fully automatic single-molecule fluorescence immunoassay analyzer, comprising an analyzer body and a sample loading chamber, and also comprising a transfer tube, wherein the transfer tube is located in the sample loading chamber, and the transfer tube is provided in multiple numbers; a knocking rod, wherein the knocking rod is located in the sample loading chamber, and the knocking rod is provided in multiple numbers, and the bottom of the transfer tube is knocked and vibrated by the knocking rod; a sample preparation unit, wherein the sample preparation unit is arranged in the sample loading chamber, and the sample solution is prepared and mixed by the sample preparation unit; a driving unit, wherein the driving unit is arranged in the sample loading chamber, and the driving unit is connected to the transfer tube and the knocking rod, and when the transfer tube transfers the sample solution, the driving unit is used to drive the knocking rod to move up and down, thereby knocking and vibrating the sample solution in the transfer tube; a positioning member, wherein the positioning member is arranged on the sample loading chamber, and the positioning member is used to limit the distance by which the detection card to be loaded is inserted into the sample loading chamber.

[0005] In some embodiments, the sample preparation unit includes a mixing component disposed on the sample loading chamber, and the sample is mixed and prepared by using the mixing component. The mixing component is provided with a discharge component, and the mixed sample is delivered into the transfer tube by using the discharge component.

[0006] In some embodiments, the adjusting accessory includes a mounting plate arranged in the sample adding chamber, on which a liquid adding tube 1 and a liquid adding tube 2 are arranged, a mixing chamber is arranged on the liquid adding tube 1 and the liquid adding tube 2, and the mixing chamber is connected with the liquid adding tube 1 and the liquid adding tube 2, a rotating shaft is rotatably arranged in the mixing chamber, and one end of the rotating shaft passes through the mixing chamber, a rotating shaft is arranged at one end of the rotating shaft located in the mixing chamber, a mixing blade is arranged on the rotating shaft, a worm gear is arranged at one end of the rotating shaft located outside the mixing chamber, a power shaft is rotatably arranged on the side wall of the sample adding chamber, and a worm is arranged on the power shaft.

[0007] In some embodiments, the discharge member includes a discharge pipe arranged on a mixing chamber, a sealing shaft is rotatably arranged on the discharge pipe, a sealing plate used in conjunction with the discharge pipe is arranged at one end of the sealing shaft located inside the discharge pipe, a discharge gear is arranged at one end of the sealing shaft located outside the discharge pipe, a discharge screw is rotatably arranged in the sample loading chamber, a limiting rod is arranged on the mixing chamber, a control rack is slidably arranged on the limiting rod, the control rack is threadedly connected to the discharge screw, and transmission gears that mesh with each other are respectively arranged on the power shaft and the discharge screw.

[0008] In some embodiments, the driving unit includes a transfer member arranged on the sample loading chamber, and the transfer member is used to move the prepared sample to the position of the detection card for loading. The transfer member is provided with a vibrator, and the vibrator is used to drive the knocking rod to move up and down to knock the bottom of the transfer tube. The transfer tube is provided with a release member, and the release member is used to add the sample in the transfer tube to the designated position on the detection card.

[0009] In some embodiments, the transfer member includes a transfer shaft rotatably arranged on the sample loading chamber, a rotating plate is arranged on the transfer shaft, the rotating plate is connected to the transfer tube, a clockwork box is arranged on the mixing chamber, the clockwork box is rotatably connected to the discharge screw, a clockwork spring is arranged in the clockwork box, one end of the clockwork spring is connected to the discharge screw, a transfer gear 1 is arranged at the end of the discharge screw away from the clockwork spring, the transfer gear 1 is connected to the discharge screw through a one-way bearing, a connecting shaft is rotatably arranged in the sample loading chamber, a transfer gear 2 is arranged on the connecting shaft and meshes with the transfer gear 1, and the diameter of the transfer gear 2 is larger than that of the transfer gear 1, and toothed belt pulleys are arranged on the connecting shaft and the transfer shaft, and a toothed belt is arranged on the toothed belt pulley.

[0010] In some embodiments, the vibrating member includes a plurality of positioning rods arranged in the sample loading chamber, a plurality of annular plates 1 are arranged on the positioning rods, annular plate 2 is arranged on the positioning rods, the knocking rod is slidably connected with annular plate 1 and annular plate 2, a locking plate is arranged on the knocking rod between annular plate 1 and annular plate 2, a knocking spring is sleeved on the knocking rod between the locking plate and annular plate 2, a pushing plate is arranged on the knocking rod at the side of annular plate 2 away from the knocking spring, an extrusion rod is arranged on the transfer tube, an extrusion rod is arranged on the extrusion rod, and an extrusion block is arranged on the extrusion rod, and the extrusion block is a triangular block.

[0011] In some embodiments, the release member includes a mounting block arranged on the transfer tube, a movable groove is provided on the mounting block, a multi-stage telescopic rod is provided in the movable groove, a blocking block is provided at one end of the multi-stage telescopic rod, the blocking block is slidably connected to the movable groove, a release spring is also provided on the multi-stage telescopic rod, a release groove is provided on the blocking block, a docking groove used in conjunction with the release groove is provided on the transfer tube, and a release protrusion used in conjunction with the blocking block is provided on the annular plate.

[0012] In some embodiments, the positioning member includes a guide rail arranged on the sample loading chamber, sliding grooves are opened on both sides of the guide rail, U-shaped plates are arranged on both sides of the guide rail, a limiting rod is slidably arranged on the U-shaped plate, a limiting block is arranged on the limiting rod, the limiting block is slidably connected to the sliding groove, a limiting plate is arranged on the limiting rod, a buffer spring is sleeved on the limiting rod between the limiting plate and the U-shaped plate, and a top plate is arranged on the limiting rod on the side of the U-shaped plate away from the buffer spring.

[0013] In some embodiments, a discharge plate is provided in the liquid adding tube, a quantitative block is rotatably provided in the liquid adding tube, the quantitative block is rotatably connected to the discharge plate, a plurality of quantitative cavities are opened on the quantitative block, a sealing plate is provided on the quantitative cavity, the sealing plate is interference fit with the quantitative cavity, and is movably connected to the liquid adding tube.

[0014] The present invention has at least the following beneficial effects: 1. Improve analysis accuracy: Use the knocking rod and knocking spring in the vibrator to perform precise vibration knocking on the transfer tube before sample transfer. This operation is intended to induce the bubbles in the solution to rise and gather on the surface of the solution. Under the continuous vibration, these bubbles will further break and release into the surrounding air, effectively reducing the bubble content in the solution. At the same time, the knocking action is directly applied to the solution itself, which can strongly cause the bubbles to break or disperse, thereby significantly reducing the probability of the existence of bubbles; Bubbles often act as "light barriers" in solutions, blocking part of the fluorescent signal, resulting in a significant reduction in the detected signal intensity. By vibrating and knocking out the bubbles, the fluorescent signal can be captured by the detector more unimpeded, thereby significantly optimizing the signal intensity and making the experimental results clearer and more intuitive; More importantly, the presence of bubbles will seriously interfere with the accurate reading of the fluorescence signal, resulting in deviation and uncertainty in the analysis results. Effectively expelling bubbles by tapping and vibrating can ensure that the fluorescence signal is read accurately, thereby greatly improving the accuracy and reliability of the analysis. 2. Avoid sample solution contamination: The transfer and preparation of sample solutions are all carried out in a strictly closed sample loading chamber. This design significantly reduces the chance of the solution coming into contact with the external environment and effectively prevents the mixing of external impurities, thereby ensuring the high purity of the sample solution. 3. Improve analysis efficiency: The mixing blades in the mixing chamber are used to mix the sample solution, which greatly shortens the operation time, improves the overall analysis efficiency, avoids errors and delays that may be caused by manual operation, and ensures the accuracy and timeliness of the analysis. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a schematic diagram of the overall structure of the present invention; Figure 2 This is a schematic diagram of the explosion structure of the sample loading chamber of the present invention; Figure 3 It is a schematic diagram of the structure of the adjusting part of the present invention; Figure 4 For the present invention Figure 3 Schematic diagram of explosion structure; Figure 5 For the present invention Figure 4 Schematic diagram of the enlarged structure of the middle A area; Figure 6 This is a schematic diagram of the structure of the drive unit of the present invention; Figure 7 For the present invention Figure 6 Another structural diagram; Figure 8 For the present invention Figure 7 Schematic diagram of the enlarged structure of the middle B area; Fig. 9 It is a schematic diagram of the structure of the release member of the present invention; Fig.10 For the present invention Fig. 9 Schematic diagram of the enlarged structure of the middle C area; Fig.11 This is a schematic diagram of the positioning member structure of the present invention; Fig.12 This is a structural diagram of Embodiment 2 of the present invention.

[0016] In the figure: 1. Analyzer body; 2. Sample loading chamber; 3. Transfer tube; 4. Knocking rod; 5. Sample preparation unit; 6. Adjustment parts; 61. Mounting plate; 62. Liquid loading tube 1; 63. Liquid loading tube 2; 64. Mixing chamber; 65. Rotating shaft; 66. Rotating shaft; 67. Mixing blade; 68. Worm gear; 69. Power shaft; 610. Worm; 7. Discharge part; 71. Discharge tube; 72. Blocking shaft; 73. Blocking plate; 74. Discharge gear; 75. Discharge screw rod; 76. Limit rod; 77. Control rack; 78. Transmission gear; 8. Drive unit; 9. Transfer part; 91. Transfer shaft; 92. Rotating plate; 93. Spring spring; 94. Spring box; 95. Transfer gear 1; 96. Transfer gear 2; 97. Connecting shaft; 98. Toothed pulley; 99. Toothed belt; 10. Vibrating member; 101. Positioning rod; 102. Annular plate 1; 103. Annular plate 2; 104. Positioning plate; 105. Percussion spring; 106. Push plate; 107. Extrusion rod; 108. Extrusion block; 11. Release member; 111. Mounting block; 112. Moving groove; 113. Multi-stage telescopic rod; 114. Blocking block; 115. Release spring; 116. Release groove; 117. Docking groove; 118. Release protrusion; 12. Positioning member; 121. Guide rail; 122. Sliding groove; 123. U-shaped plate; 124. Limiting rod; 125. Limiting block; 126. Limiting plate; 127. Buffer spring; 128. Top plate; 13. Discharge plate; 14. Dosing block; 15. Dosing chamber; 16. Sealing plate. DETAILED DESCRIPTION

[0017] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0018] Example 1: Please refer to Figure 1-Figure 11The present invention provides a technical solution: a fully automatic single-molecule fluorescence immunoassay analyzer, comprising an analyzer body 1 and a sample loading chamber 2, and also comprising a transfer tube 3, wherein the transfer tube 3 is located in the sample loading chamber 2, and the transfer tube 3 is provided with a plurality of them; a knocking rod 4, wherein the knocking rod 4 is located in the sample loading chamber 2, and the knocking rod 4 is provided with a plurality of them, and the knocking rod 4 is used to knock and vibrate the bottom of the transfer tube 3.

[0019] The sample preparation unit 5 is disposed in the sample adding chamber 2 , and the sample solution is prepared and mixed by the sample preparation unit 5 .

[0020] The advantage of setting up the sample preparation unit 5 is that it automatically completes the mixing and preparation of samples, greatly shortens the operation time, improves the overall analysis efficiency, avoids the errors and delays that may be caused by manual operation, and ensures the accuracy and timeliness of the analysis. Moreover, it can also accurately control the proportions and concentrations of various reactants, thereby optimizing the reaction conditions of fluorescent immunoassay, which helps to improve the sensitivity and specificity of the analysis and make the results more accurate and reliable.

[0021] The driving unit 8 is arranged in the sample loading chamber 2, and the driving unit 8 is connected to the transfer tube 3 and the knocking rod 4. When the transfer tube 3 transfers the sample solution, the driving unit 8 is used to drive the knocking rod 4 to move up and down, thereby knocking and vibrating the sample solution in the transfer tube 3.

[0022] The advantage of the setting of the driving unit 8 is that it can knock and vibrate the transfer tube 3 storing the sample during the sample addition process, so that the bubbles in the solution are discharged. The presence of bubbles will interfere with the reading of the fluorescent signal, resulting in inaccurate analysis results. The bubbles are discharged by knocking and vibrating, which can ensure the accurate reading of the fluorescent signal, thereby improving the accuracy of the analysis. At the same time, the bubbles will block part of the fluorescent signal, resulting in a weakening of the signal intensity. After the bubbles are discharged, the fluorescent signal can be more fully detected, thereby optimizing the signal intensity and making the results clearer. Moreover, the interference of bubbles may cause fluctuations and increased uncertainty in the experimental results. Reducing the interference of bubbles by knocking and vibrating can reduce experimental errors and make the results more stable and reliable.

[0023] The positioning member 12 is arranged on the sample loading chamber 2 , and the positioning member 12 is used to limit the distance of the detection card to be loaded with samples inserted into the sample loading chamber 2 .

[0024] The design of the positioning member 12 ensures that each sample addition can be accurately placed at the designated position of the test card, thereby avoiding errors caused by splashing of the solution or deviation from the target area. This is crucial for detection experiments that require a high degree of precision and can ensure the accuracy and reliability of the experimental results. In addition, the use of the positioning member 12 can simplify the sample addition process, allowing operators to complete the sample addition task more quickly and accurately, while reducing the time wasted due to repeated adjustments to the sample addition position, thereby improving the overall experimental efficiency. In some cases, the test solution may be harmful or corrosive to the human body. The design of the positioning member 12 helps prevent the solution from splashing, thereby reducing the risk of operators being exposed to potentially hazardous substances.

[0025] The sample preparation unit 5 comprises a preparation part 6 arranged on the sample loading chamber 2 , and the sample is mixed and prepared by the preparation part 6 . The preparation part 6 is provided with a discharge part 7 , and the mixed sample is sent into the transfer tube 3 by the discharge part 7 .

[0026] The adjusting accessory 6 includes a mounting plate 61 arranged in the sample adding chamber 2, on which a liquid adding tube 1 62 and a liquid adding tube 2 63 are arranged, and a mixing chamber 64 is arranged on the liquid adding tube 1 62 and the liquid adding tube 2 63, and the mixing chamber 64 is connected with the liquid adding tube 1 62 and the liquid adding tube 2 63, a rotating shaft 65 is rotatably arranged in the mixing chamber 64, and one end of the rotating shaft 65 passes through the mixing chamber 64, a rotating shaft 66 is arranged at one end of the rotating shaft 65 located in the mixing chamber 64, a mixing blade 67 is arranged on the rotating shaft 66, a worm gear 68 is arranged at one end of the rotating shaft 65 located outside the mixing chamber 64, a power shaft 69 is rotatably arranged on the side wall of the sample adding chamber 2, and a worm 610 is arranged on the power shaft 69.

[0027] The advantage of setting up the adjustment accessory 6 is that it automatically completes the mixing and preparation of samples, greatly shortens the operation time, improves the overall analysis efficiency, avoids the errors and delays that may be caused by manual operation, and ensures the accuracy and timeliness of the analysis. Moreover, it can also accurately control the proportions and concentrations of various reactants, thereby optimizing the reaction conditions of fluorescent immunoassay, which helps to improve the sensitivity and specificity of the analysis, making the results more accurate and reliable, and further improving the data accuracy of fluorescent immunoassay.

[0028] The discharge member 7 includes a discharge pipe 71 arranged on the mixing chamber 64, a sealing shaft 72 is rotatably arranged on the discharge pipe 71, a sealing plate 73 used in conjunction with the discharge pipe 71 is arranged at one end of the sealing shaft 72 located inside the discharge pipe 71, a discharge gear 74 is arranged at one end of the sealing shaft 72 located outside the discharge pipe 71, a discharge screw rod 75 is rotatably arranged in the sample loading chamber 2, a limiting rod 76 is arranged on the mixing chamber 64, a control rack 77 is slidably arranged on the limiting rod 76, and the control rack 77 is threadedly connected to the discharge screw rod 75, and the power shaft 69 and the discharge screw rod 75 are respectively provided with transmission gears 78 that mesh with each other.

[0029] When performing fluorescent immunoassay, first, the fluorescent reagent and the sample to be tested are injected into the mixing chamber 64 from the liquid adding tube 1 62 and the liquid adding tube 2 63 respectively, and then the power shaft 69 is rotated to drive the worm 610 located on the power shaft 69 to rotate, and the rotation of the worm 610 drives the worm gear 68 to rotate. The rotation of the worm gear 68 also drives the rotation shaft 65 to rotate, and the rotation of the rotating shaft 65 drives the rotating shaft 66 connected to it to rotate, and the rotation of the rotating shaft 66 drives the mixing blade 67 arranged thereon to rotate, and the rotation of the mixing blade 67 drives the sample solution located in the mixing chamber 64 to mix.

[0030] As the power shaft 69 rotates, the transmission gear 78 thereon is driven to rotate. The rotation of the transmission gear 78 drives the transmission gear 78 located on the discharge screw rod 75 to rotate, thereby driving the discharge screw rod 75 to rotate. The rotation of the discharge screw rod 75 drives the control rack 77 to rise. During the continuous rotation of the power shaft 69, the control rack 77 engages with the discharge gear 74, thereby driving the sealing shaft 72 and the sealing plate 73 to rotate, so that the discharge pipe 71 is unblocked. At this time, the mixed sample solution is transferred from the discharge pipe 71 to the transfer pipe 3.

[0031] The driving unit 8 includes a transfer member 9 arranged on the sample loading chamber 2, and the transfer member 9 is used to move the prepared sample to the position of the detection card for loading. The transfer member 9 is provided with a vibrator 10, and the vibrator 10 is used to drive the knocking rod 4 to move up and down to knock the bottom of the transfer tube 3. The transfer tube 3 is provided with a release member 11, and the release member 11 is used to add the sample in the transfer tube 3 to the specified position on the detection card.

[0032] The transfer member 9 includes a transfer shaft 91 rotatably arranged on the sample loading chamber 2, a rotating plate 92 is arranged on the transfer shaft 91, and the rotating plate 92 is connected to the transfer tube 3, a clockwork box 94 is arranged on the mixing chamber 64, and the clockwork box 94 is rotatably connected to the discharge screw rod 75, a clockwork spring 93 is arranged in the clockwork box 94, one end of the clockwork spring 93 is connected to the discharge screw rod 75, and a transfer gear 1 95 is arranged at one end of the discharge screw rod 75 away from the clockwork spring 93, and the transfer gear 1 95 is connected to the discharge screw rod 75 through a one-way bearing, a connecting shaft 97 is rotatably arranged in the sample loading chamber 2, a transfer gear 2 96 engaged with the transfer gear 1 95 is arranged on the connecting shaft 97, and the diameter of the transfer gear 2 96 is larger than that of the transfer gear 1 95, and a toothed belt pulley 98 is arranged on both the connecting shaft 97 and the transfer shaft 91, and a toothed belt 99 is arranged on the toothed belt pulley 98.

[0033] The vibrating member 10 includes a plurality of positioning rods 101 arranged in the sample loading chamber 2, a plurality of annular plates 102 are arranged on the positioning rods 101, an annular plate 2 103 is arranged on the positioning rods 101, the knocking rod 4 is slidably connected with the annular plate 102 and the annular plate 2 103, a locking plate 104 is arranged on the knocking rod 4 between the annular plate 102 and the annular plate 2 103, a knocking spring 105 is sleeved on the knocking rod 4 between the locking plate 104 and the annular plate 2 103, a pushing plate 106 is arranged on the knocking rod 4 on the side of the annular plate 2 103 away from the knocking spring 105, an extrusion rod 107 is arranged on the transfer tube 3, an extrusion block 108 is arranged on the extrusion rod 107, and the extrusion block 108 is a triangular block.

[0034] The advantage of setting up the vibration part 10 is that it can knock and vibrate the transfer tube 3 storing the sample during the sample addition process, so that the bubbles in the solution are discharged. The presence of bubbles will interfere with the reading of the fluorescence signal and cause inaccurate analysis results. By knocking and vibrating to discharge the bubbles, the accurate reading of the fluorescence signal can be ensured, thereby improving the accuracy of the analysis.

[0035] The release member 11 includes a mounting block 111 arranged on the transfer tube 3, the mounting block 111 is provided with a movable groove 112, a multi-stage telescopic rod 113 is arranged in the movable groove 112, a blocking block 114 is arranged at one end of the multi-stage telescopic rod 113, the blocking block 114 is slidably connected with the movable groove 112, and a release spring 115 is also provided on the multi-stage telescopic rod 113, a release groove 116 is provided on the blocking block 114, a docking groove 117 used in conjunction with the release groove 116 is provided on the transfer tube 3, and a release protrusion 118 used in conjunction with the blocking block 114 is provided on the annular plate 102.

[0036] While the power shaft 69 is rotating, the clockwork spring 93 is synchronously driven to accumulate force. After the solution is injected into the transfer tube 3, the staff manually releases the power shaft 69, and the clockwork spring 93 is released. At this time, the discharge screw 75 rotates in the opposite direction, thereby driving the sealing plate 73 to reset, and at the same time driving the transfer gear 1 95 to rotate. The rotation of the transfer gear 1 95 drives the transfer gear 2 96 engaged with it to rotate. The rotation of the transfer gear 2 96 drives the connecting shaft 97 to rotate. The connecting shaft 97 rotates and synchronously drives the transfer shaft 91 to rotate through the toothed pulley 98 and the toothed belt 99. The rotation of the transfer shaft 91 drives the rotating plate 92 and the transfer tube 3 to rotate synchronously.

[0037] When the transfer tube 3 moves, the extrusion rod 107 and the extrusion block 108 arranged thereon are driven to move synchronously. The movement of the extrusion block 108 pushes the push plate 106 to descend, thereby driving the knocking rod 4 to descend. When the extrusion block 108 is out of contact with the push plate 106, the knocking rod 4 is reset again under the push of the knocking spring 105, and generates knocking vibration on the transfer tube 3, so that the bubbles in the solution are discharged.

[0038] When the transfer tube 3 moves to the designated position, the blocking block 114 contacts the release protrusion 118, so that the blocking block 114 is pushed, thereby docking the release groove 116 on the blocking block 114 with the docking groove 117 on the transfer tube 3, and dripping the solution to the designated position of the test card.

[0039] The positioning member 12 includes a guide rail 121 arranged on the sample loading bin 2, and sliding grooves 122 are opened on both sides of the guide rail 121. U-shaped plates 123 are arranged on both sides of the guide rail 121. A limiting rod 124 is slidably arranged on the U-shaped plate 123, and a limiting block 125 is arranged on the limiting rod 124. The limiting block 125 is slidably connected to the sliding groove 122. A limiting plate 126 is arranged on the limiting rod 124, and a buffer spring 127 is sleeved on the limiting rod 124 between the limiting plate 126 and the U-shaped plate 123. A top plate 128 is arranged on the limiting rod 124 on the side of the U-shaped plate 123 away from the buffer spring 127.

[0040] The advantage of setting the positioning member 12 is that it ensures that each sample addition can be accurately placed at the designated position of the test card, thereby avoiding errors caused by solution splashing or deviation from the target area. This is crucial for detection experiments that require high precision and can ensure the accuracy and reliability of the experimental results.

[0041] When inserting the test card, when the test card is held by the limiting block 125 , the solution detection area of ​​the test card is directly below the transfer tube 3 , thereby ensuring the accuracy of solution sampling. After the sampling is completed, the test card is further pushed so that the test card enters the analyzer body 1 .

[0042] During use, when conducting fluorescent immunoassay, first, the fluorescent reagent and the sample to be tested are injected into the mixing chamber 64 from the liquid adding tube 1 62 and the liquid adding tube 2 63 respectively, and then the power shaft 69 is rotated to drive the worm 610 located on the power shaft 69 to rotate, and the rotation of the worm 610 drives the worm gear 68 to rotate, and the rotation of the worm gear 68 drives the rotating shaft 65 to rotate, and the rotation of the rotating shaft 65 drives the rotating shaft 66 connected to it to rotate, and the rotation of the rotating shaft 66 drives the mixing blade 67 arranged thereon to rotate, and the rotation of the mixing blade 67 drives the sample solution located in the mixing chamber 64 to mix.

[0043] As the power shaft 69 rotates, the transmission gear 78 thereon is driven to rotate. The rotation of the transmission gear 78 drives the transmission gear 78 located on the discharge screw rod 75 to rotate, thereby driving the discharge screw rod 75 to rotate. The rotation of the discharge screw rod 75 drives the control rack 77 to rise. During the continuous rotation of the power shaft 69, the control rack 77 engages with the discharge gear 74, thereby driving the sealing shaft 72 and the sealing plate 73 to rotate, so that the discharge pipe 71 is unblocked. At this time, the mixed sample solution is transferred from the discharge pipe 71 to the transfer pipe 3.

[0044] While the power shaft 69 is rotating, the clockwork spring 93 is synchronously driven to accumulate force. After the solution is injected into the transfer tube 3, the staff manually releases the power shaft 69, and the clockwork spring 93 is released. At this time, the discharge screw 75 rotates in the opposite direction, thereby driving the sealing plate 73 to reset, and at the same time driving the transfer gear 1 95 to rotate. The rotation of the transfer gear 1 95 drives the transfer gear 2 96 engaged with it to rotate. The rotation of the transfer gear 2 96 drives the connecting shaft 97 to rotate. The connecting shaft 97 rotates and synchronously drives the transfer shaft 91 to rotate through the toothed pulley 98 and the toothed belt 99. The rotation of the transfer shaft 91 drives the rotating plate 92 and the transfer tube 3 to rotate synchronously.

[0045] When the transfer tube 3 moves, the extrusion rod 107 and the extrusion block 108 arranged thereon are driven to move synchronously. The movement of the extrusion block 108 pushes the push plate 106 to descend, thereby driving the knocking rod 4 to descend. When the extrusion block 108 is out of contact with the push plate 106, the knocking rod 4 is reset again under the push of the knocking spring 105, and generates knocking vibration on the transfer tube 3, so that the bubbles in the solution are discharged.

[0046] When the transfer tube 3 moves to the designated position, the blocking block 114 contacts the release protrusion 118, so that the blocking block 114 is pushed, thereby docking the release groove 116 on the blocking block 114 with the docking groove 117 on the transfer tube 3, and dripping the solution to the designated position of the test card.

[0047] When inserting the test card, when the test card is held by the limiting block 125 , the solution detection area of ​​the test card is directly below the transfer tube 3 , thereby ensuring the accuracy of solution sampling. After the sampling is completed, the test card is further pushed so that the test card enters the analyzer body 1 .

[0048] Example 2: Please refer to Fig.12 The present invention provides a technical solution: a discharge plate 13 is arranged in a liquid adding tube 62, a quantitative block 14 is rotatably arranged in the liquid adding tube 62, the quantitative block 14 is rotatably connected to the discharge plate 13, a plurality of quantitative cavities 15 are opened on the quantitative block 14, a sealing plate 16 is arranged on the quantitative cavity 15, the sealing plate 16 is interference fit with the quantitative cavity 15, and is movably connected to the liquid adding tube 62. The advantage of this design is that when multiple tests of a single sample are required, the fluorescent reagent can be quickly added by rotating the quantitative cavity 15, which greatly reduces the work pressure of the staff.

[0049] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0050] While the embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that many changes, modifications, substitutions and variations can be made to the embodiments without departing from the principles and spirit of the invention.

Claims

1. A fully automatic single molecule fluorescence immunoassay analyzer, comprising an analyzer body (1) and a sample loading chamber (2), characterized in that: Also includes: A transfer tube (3), the transfer tube (3) being located in the sample loading chamber (2), and a plurality of the transfer tubes (3) being provided; A knocking rod (4), the knocking rod (4) being located in the sample loading chamber (2), and a plurality of the knocking rods (4) being provided; the knocking rod (4) is used to knock and vibrate the bottom of the transfer tube (3); A sample preparation unit (5), the sample preparation unit (5) being arranged in the sample loading chamber (2), and the sample solution is prepared and mixed by the sample preparation unit (5); A driving unit (8), wherein the driving unit (8) is arranged in the sample loading chamber (2), and the driving unit (8) is connected to the transfer tube (3) and the knocking rod (4). When the transfer tube (3) transfers the sample solution, the driving unit (8) drives the knocking rod (4) to move up and down, thereby knocking and vibrating the sample solution in the transfer tube (3); A positioning member (12), wherein the positioning member (12) is arranged on the sample loading chamber (2), and the distance of the detection card to be loaded with samples inserted into the sample loading chamber (2) is limited by the positioning member (12).

2. The fully automatic single molecule fluorescence immunoassay analyzer according to claim 1, characterized in that: The sample preparation unit (5) comprises a preparation component (6) arranged on the sample loading chamber (2), and the sample is mixed and prepared by using the preparation component (6). The preparation component (6) is provided with a discharge component (7), and the mixed sample is sent into the transfer tube (3) by using the discharge component (7).

3. The fully automatic single molecule fluorescence immunoassay analyzer according to claim 2, characterized in that: The adjusting component (6) comprises a mounting plate (61) arranged in the sample adding chamber (2); a first liquid adding pipe (62) and a second liquid adding pipe (63) are arranged on the mounting plate (61); a mixing chamber (64) is arranged on the first liquid adding pipe (62) and the second liquid adding pipe (63); the mixing chamber (64) is communicated with the first liquid adding pipe (62) and the second liquid adding pipe (63); a rotating shaft (65) is rotatably arranged in the mixing chamber (64); one end of the rotating shaft (65) passes through the mixing chamber (64); a rotating shaft (66) is arranged at one end of the rotating shaft (65) located in the mixing chamber (64); a mixing blade (67) is arranged on the rotating shaft (66); a worm gear (68) is arranged at one end of the rotating shaft (65) located outside the mixing chamber (64); a power shaft (69) is rotatably arranged on the side wall of the sample adding chamber (2); a worm (610) is arranged on the power shaft (69).

4. The fully automatic single molecule fluorescence immunoassay analyzer according to claim 3, characterized in that: The discharge member (7) comprises a discharge pipe (71) arranged on the mixing chamber (64); a blocking shaft (72) is rotatably arranged on the discharge pipe (71); a blocking plate (73) used in conjunction with the discharge pipe (71) is arranged at one end of the blocking shaft (72) located inside the discharge pipe (71); a discharge gear (74) is arranged at one end of the blocking shaft (72) located outside the discharge pipe (71); a discharge screw rod (75) is rotatably arranged in the sample loading chamber (2); a limit rod (76) is arranged on the mixing chamber (64); a control rack (77) is slidably arranged on the limit rod (76); the control rack (77) is threadedly connected to the discharge screw rod (75); and transmission gears (78) meshing with each other are respectively arranged on the power shaft (69) and the discharge screw rod (75).

5. The fully automatic single molecule fluorescence immunoassay analyzer according to claim 4, characterized in that: The driving unit (8) comprises a transfer member (9) arranged on the sample loading chamber (2), and the transfer member (9) is used to move the prepared sample to the position of the detection card for loading. The transfer member (9) is provided with a vibrating member (10), and the vibrating member (10) is used to drive the knocking rod (4) to move up and down to knock the bottom of the transfer tube (3). The transfer tube (3) is provided with a releasing member (11), and the releasing member (11) is used to add the sample in the transfer tube (3) to a designated position on the detection card.

6. The fully automatic single molecule fluorescence immunoassay analyzer according to claim 5, characterized in that: The transfer member (9) comprises a transfer shaft (91) rotatably arranged on the sample loading chamber (2), a rotating plate (92) being arranged on the transfer shaft (91), the rotating plate (92) being connected to the transfer tube (3), a spring box (94) being arranged on the mixing chamber (64), the spring box (94) being rotatably connected to the discharge screw rod (75), a spring spring (93) being arranged in the spring box (94), one end of the spring spring (93) being connected to the discharge screw rod (75), the discharge screw rod (75) being away from the spring spring (93) A transfer gear 1 (95) is provided at one end, and the transfer gear 1 (95) is connected to the discharge screw rod (75) via a one-way bearing. A connecting shaft (97) is rotatably provided in the sample loading chamber (2), and a transfer gear 2 (96) is provided on the connecting shaft (97) and engages with the transfer gear 1 (95), and the diameter of the transfer gear 2 (96) is larger than that of the transfer gear 1 (95). A toothed belt wheel (98) is provided on both the connecting shaft (97) and the transfer shaft (91), and a toothed belt wheel (98) is provided on the toothed belt wheel (98).

7. The fully automatic single molecule fluorescence immunoassay analyzer according to claim 6, characterized in that: The vibrating member (10) comprises a plurality of positioning rods (101) arranged in the sample loading chamber (2); a plurality of annular plates (102) are arranged on the positioning rods (101); an annular plate (103) is arranged on the positioning rods (101); the knocking rod (4) is slidably connected with the annular plate (102) and the annular plate (103); a positioning plate (104) is arranged on the knocking rod (4) between the annular plate (102) and the annular plate (103); a knocking spring (105) is sleeved on the knocking rod (4) between the positioning plate (104) and the annular plate (103); a pushing plate (106) is arranged on the knocking rod (4) at a side of the annular plate (103) away from the knocking spring (105); an extrusion rod (107) is arranged on the transfer tube (3); an extrusion block (108) is arranged on the extrusion rod (107); and the extrusion block (108) is a triangular block.

8. The fully automatic single molecule fluorescence immunoassay analyzer according to claim 7, characterized in that: The release member (11) comprises a mounting block (111) arranged on the transfer tube (3), the mounting block (111) being provided with a movable groove (112), a multi-stage telescopic rod (113) being arranged in the movable groove (112), a blocking block (114) being arranged at one end of the multi-stage telescopic rod (113), the blocking block (114) being slidably connected to the movable groove (112), a release spring (115) being arranged on the multi-stage telescopic rod (113), a release groove (116) being arranged on the blocking block (114), a docking groove (117) being arranged on the transfer tube (3) for use with the release groove (116), and a release protrusion (118) being arranged on the annular plate (102) for use with the blocking block (114).

9. The fully automatic single molecule fluorescence immunoassay analyzer according to claim 8, characterized in that: The positioning member (12) comprises a guide rail (121) arranged on the sample loading chamber (2), the guide rail (121) having sliding grooves (122) on both sides, U-shaped plates (123) on both sides of the guide rail (121), a limiting rod (124) slidably arranged on the U-shaped plate (123), a limiting block (125) arranged on the limiting rod (124), the limiting block (125) being slidably connected to the sliding groove (122), a limiting plate (126) being arranged on the limiting rod (124), a buffer spring (127) being sleeved on the limiting rod (124) located between the limiting plate (126) and the U-shaped plate (123), and a top plate (128) being arranged on the limiting rod (124) on a side of the U-shaped plate (123) away from the buffer spring (127).

10. The fully automatic single molecule fluorescence immunoassay analyzer according to claim 9, characterized in that: A discharge plate (13) is arranged in the liquid adding tube (62), a quantitative block (14) is rotatably arranged in the liquid adding tube (62), the quantitative block (14) is rotatably connected to the discharge plate (13), a plurality of quantitative cavities (15) are formed on the quantitative block (14), a sealing plate (16) is arranged on the quantitative cavity (15), the sealing plate (16) is interference fit with the quantitative cavity (15), and is movably connected to the liquid adding tube (62).

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