Preimplantation genetic diagnosis embryo biopsy device

Abstract

The present invention discloses a preimplantation genetic diagnosis embryo biopsy device, which relates to the technical field of embryo biopsy sampling. The preimplantation genetic diagnosis embryo biopsy device includes a microscope for embryo diagnostic biopsy. The present invention achieves adsorption and fixation of the embryo and adsorption sampling of the biopsy material through the mutual cooperation of a rotating component, a moving component, a connecting component, and a control component. During the entire sampling process, because the negative pressure of the negative pressure chamber inside the second pressure cylinder is always greater than the pressure inside the first pressure cylinder, the interconnection between the first connecting tube and the second connecting tube makes the adsorption force of the suction pipe on the embryo greater than the adsorption force on the biopsy material after the puncture needle is inserted. This prevents the embryo from swinging and shifting during the adsorption sampling process due to the adsorption pressure on the biopsy material being greater than the pressure on the embryo to adsorb and fix it, further ensuring stability during the puncture biopsy sampling process.

Description

Technical Field

[0001] The present invention relates to the technical field of embryo biopsy sampling, in particular to an embryo biopsy device for pre-implantation genetic diagnosis. Background Art

[0002] Before embryo implantation, genetic diagnosis is required through embryo biopsy sampling technology, which can detect whether the embryo carries the disease-causing gene. If the embryo is found to carry the disease-causing gene, it can avoid implanting the embryo with the genetic disease into the mother, thereby reducing the risk of transmission of the genetic disease in the family.

[0003] During the genetic diagnosis of embryos using embryo biopsy sampling technology, the experimenter transfers the ICSI culture dish under a microscope and samples the biopsy material inside the embryo through the insertion and adsorption of the puncture needle. During the insertion of the puncture needle, in order to avoid the embryo from being moved by force during the insertion process, the embryo needs to be assisted and fixed by a straw. During the entire auxiliary fixation and insertion and adsorption process, it is inconvenient to operate and control the difference between the adsorption pressure of the straw on the embryo and the adsorption pressure of the biopsy material after the puncture needle is inserted. If the adsorption pressure of the straw on the embryo is lower than the adsorption pressure of the biopsy material after the puncture needle is inserted, the embryo will swing and shift due to the pressure difference during the insertion and adsorption of the puncture needle, affecting the effect of the puncture biopsy sampling. For this reason, we propose an embryo biopsy device for preimplantation genetic diagnosis. Summary of the Invention

[0004] The object of the present invention is to provide a pre-implantation genetic diagnosis embryo biopsy device to solve the problems raised in the above background technology.

[0005] To achieve the above-mentioned object, the present invention provides the following technical solutions: a pre-implantation genetic diagnosis embryo biopsy device, comprising a microscope for embryo diagnostic biopsy, the microscope being provided with a carrier for carrying and placing an embryo culture dish, the microscope being provided with a puncture needle for inserting embryos for sampling during embryo biopsy sampling and a pipette needle for adsorbing and fixing the embryos, and further comprising a connecting assembly provided on the microscope for connecting the puncture needle and the pipette needle, the connecting assembly comprising a mounting frame fixed to the microscope, the mounting frame being symmetrically provided with a first connecting tube and a second connecting tube, the puncture needle being capable of being inserted into the microscope and the pipette needle being capable of being adsorbed and fixed. The suction needle is detachably mounted on the front end of the first connecting tube, and the first connecting tube and the second connecting tube are respectively provided with a first control valve and a second control valve for controlling the communication between the pipelines. Two sets of symmetrically arranged mounting plates are fixed on the mounting bracket, and the front side of the mounting plate is rotatably connected to an adjustment seat through a rotating assembly. The adjustment seat is connected to a mounting seat through a moving assembly. The mounting seat is provided with a mounting assembly for assisting the clamping and installation of the first connecting tube or the second connecting tube, and the mounting bracket is provided with a control assembly for pressure control during the use of the puncture needle and the suction needle.

[0006] Preferably, the control component includes a first pressure cylinder and a second pressure cylinder arranged on the front side of the mounting frame, and the aperture and capacity inside the first pressure cylinder and the second pressure cylinder are set to be the same, the rear end of the first connecting tube is connected to the upper end of the first pressure cylinder, and the interior of the second pressure cylinder is slidably connected to a piston plate for dividing the internal chamber of the second pressure cylinder. The interior of the second pressure cylinder forms a negative pressure chamber and a normal pressure chamber under the division of the piston plate, and the negative pressure chamber is above the normal pressure chamber. The lower end of the second pressure cylinder is provided with a lifting component for adjusting the lifting and lowering of the piston plate, the rear end of the second connecting tube is connected to the upper end of the second pressure cylinder, and the second connecting tube is connected to the interior of the negative pressure chamber, and the mounting frame is provided with a pumping and pressing component for pumping pressure into the first and second pressure cylinders.

[0007] Preferably, the lifting assembly includes a first threaded sleeve fixed to the lower end of the second pressure cylinder, the first threaded sleeve is threadedly engaged with a first screw rod, one end of the first screw rod is rotatably connected to the piston plate, and the other end of the first screw rod is fixed with a rotating pin for driving the first screw rod to rotate.

[0008] Preferably, the pressure-extraction assembly includes piston cylinders that are fixed on the first pressure cylinder and the second pressure cylinder, the piston cylinders are connected to the interior of the negative pressure chamber, the two groups of piston cylinders are slidably connected with piston rods, a push plate is provided on the front side of the mounting frame, one end of the two groups of piston rods is fixed to the push plate, and the mounting frame is provided with a pushing assembly for pushing the push plate and a guide assembly for guiding during the pushing process.

[0009] Preferably, the pushing assembly includes a threaded rod rotatably connected to the mounting frame, a threaded tube is threadedly engaged on the threaded rod, one end of the threaded tube is fixed to the push plate, and a driving motor for driving the threaded rod is installed on the mounting frame.

[0010] Preferably, the guide assembly includes two groups of sleeves fixed on the push plate, the two groups of sleeves are symmetrically arranged on both sides of the threaded tube, and the two groups of sleeves are slidably connected to a sliding rod, one end of the sliding rod is fixed to the mounting frame.

[0011] Preferably, the rotating assembly includes a rotating shaft rotatably connected to the mounting plate, one end of the rotating shaft is fixed to the back of the adjustment seat, an operating box is fixed to the mounting plate, the other end of the rotating shaft is located inside the operating box and is fixed with a worm gear, an operating shaft is rotatably connected to the operating box, a worm is fixed on the operating shaft, the worm and the worm gear are meshed with each other, and one end of the operating shaft is located outside the operating box and is fixed with a mounting pin.

[0012] Preferably, the moving assembly includes a slide groove opened on the adjustment seat, a slider is slidably connected to the slide groove, the mounting seat is fixed to the front side of the slider, a second threaded sleeve is fixed on the slider, an L-shaped frame is fixed on one side of the adjustment seat, a second screw rod is rotatably connected to the L-shaped frame, the second screw rod and the second threaded sleeve are engaged with each other, and a fixing pin for driving the second screw rod is fixed to one end of the second screw rod.

[0013] Preferably, the mounting assembly includes a connecting plate fixed on the mounting seat, and the connecting plate is provided with a slot for clamping and fixing the first connecting pipe or the second connecting pipe.

[0014] Compared with the prior art, the present invention has the following beneficial effects:

[0015] The present invention completes the adsorption and fixation of the embryo and the adsorption sampling of the biopsy material through the mutual cooperation of the rotating component, the moving component, the connecting component and the control component. During the entire sampling process, since the negative pressure of the negative pressure chamber inside the second pressure cylinder is always greater than the pressure inside the first pressure cylinder, the first connecting tube and the second connecting tube are connected to each other, so that the adsorption force of the straw on the embryo is greater than the adsorption force on the biopsy material after the puncture needle is inserted, thereby avoiding the embryo swinging and displacement caused by the adsorption pressure on the biopsy material being greater than the pressure for adsorption and fixation of the embryo during the adsorption sampling process, and further ensuring the stability of the puncture biopsy sampling process. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a schematic diagram of the overall appearance structure of the present invention;

[0017] Figure 2This is a schematic diagram of the connection assembly structure of the present invention;

[0018] Figure 3 This is a schematic diagram of the installation assembly structure of the present invention;

[0019] Figure 4 It is a schematic diagram of the structure of the mobile component of the present invention;

[0020] Figure 5 It is a schematic structural diagram of the rotating assembly of the present invention;

[0021] Figure 6 This is a schematic diagram of the control component structure of the present invention;

[0022] Figure 7 It is a schematic structural diagram of the pushing component and the guiding component of the present invention;

[0023] Figure 8 It is a schematic structural diagram of the lifting assembly of the present invention;

[0024] Figure 9 It is a schematic diagram of the internal structure of the first pressure cylinder and the second pressure cylinder of the present invention.

[0025] In the figure: 101 - microscope; 102 - carrying platform; 103 - puncture needle; 104 - pipette needle; 201 - mounting bracket; 202 - first connecting pipe; 203 - second connecting pipe; 204 - first control valve; 205 - second control valve; 3 - mounting plate; 4 - adjustment seat; 501 - rotating shaft; 502 - operating box; 503 - worm gear; 504 - operating shaft; 505 - worm; 506 - mounting pin; 6 - mounting seat; 701 - connecting plate; 702 - slot; 801 - slideway; 802-slider; 803-second threaded sleeve; 804-L-shaped frame; 805-second screw rod; 806-fixing pin; 901-first pressure cylinder; 902-second pressure cylinder; 1001-piston cylinder; 1002-piston rod; 1003-push plate; 1101-threaded rod; 1102-threaded tube; 1103-drive motor; 12-piston plate; 1301-first threaded sleeve; 1302-first screw rod; 1303-rotating pin; 1401-sleeve; 1402-slider. DETAILED DESCRIPTION

[0026] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0027] Example 1, please refer to Figures 1-9The figure shows a preimplantation genetic diagnosis embryo biopsy device, comprising a microscope 101 for embryo diagnostic biopsy, a carrier 102 for placing an embryo culture dish, a puncture needle 103 for inserting an embryo sample during the embryo biopsy sampling process, and a pipette needle 104 for adsorbing and fixing the embryo.

[0028] It is worth noting that the microscope 101 and the carrier 102 are conventional operating equipment during the embryo biopsy sampling process. As known technologies in this application, their working principles and operating methods will not be further elaborated here.

[0029] The microscope 101 further includes a connecting assembly for connecting the puncture needle 103 and the pipette needle 104. The connecting assembly includes a mounting frame 201 fixed to the microscope 101, and a first connecting tube 202 and a second connecting tube 203 are symmetrically mounted on the mounting frame 201.

[0030] It should be noted that the first connecting pipe 202 and the second connecting pipe 203 have the same length and aperture to ensure that the pressure changes are consistent when the same connection is made after subsequent pressure extraction.

[0031] The puncture needle 103 is detachably mounted on the front end of the first connecting tube 202, and the pipette needle 104 is detachably mounted on the front end of the second connecting tube 203. The first connecting tube 202 and the second connecting tube 203 are respectively provided with a first control valve 204 and a second control valve 205 for controlling the communication between the pipelines. Two sets of symmetrically arranged mounting plates 3 are fixed on the mounting frame 201. The front side of the mounting plate 3 is rotatably connected to an adjusting seat 4 through a rotating assembly. The adjusting seat 4 is connected to a mounting seat 6 through a moving assembly. The mounting seat 6 is provided with a mounting assembly for assisting the first connecting tube 202 or the second connecting tube 203 in clamping and installing. The mounting frame 201 is provided with a control assembly for controlling the pressure of the puncture needle 103 and the pipette needle 104 during use.

[0032] It should be noted here that the adsorption and fixation of the embryo and the adsorption sampling of the biopsy are completed through the mutual cooperation of the rotating component, the moving component, the connecting component and the control component. During the entire sampling process, since the negative pressure of the negative pressure chamber inside the second pressure cylinder 902 is always greater than the pressure inside the first pressure cylinder 901, the first connecting tube 202 and the second connecting tube 203 are connected to each other, so that the adsorption force of the pipette needle 104 on the embryo is greater than the adsorption force of the biopsy after the puncture needle 103 is inserted, thereby avoiding the swinging and displacement of the embryo during the adsorption sampling process due to the adsorption pressure on the biopsy being greater than the pressure on the adsorption and fixation of the embryo, and further ensuring the stability of the puncture biopsy sampling process.

[0033] Preferably, the control component includes a first pressure cylinder 901 and a second pressure cylinder 902 arranged on the front side of the mounting frame 201, and the aperture and capacity inside the first pressure cylinder 901 and the second pressure cylinder 902 are set to be the same, the rear end of the first connecting tube 202 is communicated with the upper end of the first pressure cylinder 901, and the interior of the second pressure cylinder 902 is slidably connected to the piston plate 12 for dividing the internal chamber of the second pressure cylinder 902. The interior of the second pressure cylinder 902 forms a negative pressure chamber and a normal pressure chamber under the division of the piston plate 12, and the negative pressure chamber is above the normal pressure chamber. The lower end of the second pressure cylinder 902 is provided with a lifting component for adjusting the lifting and lowering of the piston plate 12, the rear end of the second connecting tube 203 is communicated with the upper end of the second pressure cylinder 902, and the second connecting tube 203 is communicated with the interior of the negative pressure chamber, and a pumping and pressing component for pumping pressure inside the first pressure cylinder 901 and the second pressure cylinder 902 is provided on the mounting frame 201;

[0034] It should be noted here that: through the mutual cooperation of the pushing assembly and the guiding assembly, the push plate 1003 is forced to move toward the mounting frame 201. During the movement of the push plate 1003, the two groups of piston rods 1002 are pulled outward from the two groups of piston cylinders 1001 respectively. In the process of pulling the piston rods 1002 outward, negative pressure is generated inside the first pressure cylinder 901 and inside the negative pressure cavity of the second pressure cylinder 902. Due to the dividing effect of the piston plate 12 inside the second pressure cylinder 902, the capacity of the negative pressure cavity inside the second pressure cylinder 902 is smaller than the capacity inside the first pressure cylinder 901, and the two groups of piston rods 1002 slide outward the same distance on the two groups of piston cylinders 1001 respectively, so that the negative pressure pressure inside the negative pressure cavity inside the second pressure cylinder 902 is greater than the pressure inside the first pressure cylinder 901.

[0035] Preferably, the lifting assembly includes a first threaded sleeve 1301 fixed to the lower end of the second pressure cylinder 902, a first screw rod 1302 being threadedly engaged with the first threaded sleeve 1301, one end of the first screw rod 1302 being rotatably connected to the piston plate 12, and a rotating pin 1303 for driving the first screw rod 1302 to rotate is fixed to the other end of the first screw rod 1302;

[0036] It should be noted here that: the first screw rod 1302 is driven to rotate by the rotating pin 1303. During the rotation of the first screw rod 1302, the piston plate 12 is caused to move up and down inside the second pressure cylinder 902 through the mutual engagement transmission between the first screw rod 1302 and the first threaded sleeve 1301. Through the lifting and lowering movement of the piston plate 12, the capacity of the negative pressure chamber inside the second pressure cylinder 902 is adjusted. Through the adjustment of the capacity, the pressure difference between the capacity chamber and the first pressure cylinder 901 is controlled.

[0037] Preferably, the pumping assembly includes piston cylinders 1001 that are fixed to the first pressure cylinder 901 and the second pressure cylinder 902, the piston cylinders 1001 being connected to the interior of the negative pressure chamber, piston rods 1002 being slidably connected to the two sets of piston cylinders 1001, a push plate 1003 being provided on the front side of the mounting frame 201, one end of the two sets of piston rods 1002 being fixed to the push plate 1003, and a pushing assembly for pushing the push plate 1003 and a guide assembly for guiding during the pushing process being provided on the mounting frame 201;

[0038] It should be noted here that: through the mutual cooperation of the pushing assembly and the guiding assembly, the push plate 1003 is forced to move toward the mounting frame 201. During the movement of the push plate 1003, the two groups of piston rods 1002 are pulled outward from the two groups of piston cylinders 1001 respectively. In the process of pulling the piston rods 1002 outward, negative pressure is generated inside the first pressure cylinder 901 and inside the negative pressure cavity of the second pressure cylinder 902. Due to the dividing effect of the piston plate 12 inside the second pressure cylinder 902, the capacity of the negative pressure cavity inside the second pressure cylinder 902 is smaller than the capacity inside the first pressure cylinder 901, and the two groups of piston rods 1002 slide outward the same distance on the two groups of piston cylinders 1001 respectively, so that the negative pressure pressure inside the negative pressure cavity inside the second pressure cylinder 902 is greater than the pressure inside the first pressure cylinder 901.

[0039] Preferably, the pushing assembly includes a threaded rod 1101 rotatably connected to the mounting frame 201, a threaded tube 1102 being threadedly engaged on the threaded rod 1101, one end of the threaded tube 1102 being fixed to the push plate 1003, and a drive motor 1103 for driving the threaded rod 1101 being installed on the mounting frame 201; the guiding assembly includes two sets of sleeves 1401 fixed to the push plate 1003, the two sets of sleeves 1401 being symmetrically arranged on both sides of the threaded tube 1102, and a sliding rod 1402 being slidably connected to the two sets of sleeves 1401, one end of the sliding rod 1402 being fixed to the mounting frame 201;

[0040] It should be noted here that: the threaded rod 1101 is driven to rotate by the driving motor 1103. During the rotation of the threaded rod 1101, the push plate 1003 is moved after being subjected to force through the mutual engagement transmission between the threaded rod 1101 and the threaded tube 1102 and the sliding guiding effect of the two sets of sleeves 1401 and the two sets of sliding rods 1402.

[0041] Preferably, the rotating assembly includes a rotating shaft 501 rotatably connected to the mounting plate 3, one end of the rotating shaft 501 is fixed to the back of the adjustment seat 4, an operating box 502 is fixed to the mounting plate 3, the other end of the rotating shaft 501 is located inside the operating box 502 and is fixed with a worm gear 503, an operating shaft 504 is rotatably connected to the operating box 502, a worm 505 is fixed to the operating shaft 504, the worm 505 and the worm gear 503 are meshed with each other, and one end of the operating shaft 504 is located outside the operating box 502 and is fixed with a mounting pin 506;

[0042] It should be noted here that: the operating shaft 504 and the worm 505 at one end of the operating shaft 504 are driven to rotate by the mounting pin 506. During the rotation of the worm 505, the rotating shaft 501 is driven to rotate through the mutual engagement transmission between the worm 505 and the worm wheel 503. During the rotation of the rotating shaft 501, the adjusting seat 4 and the first connecting tube 202 and the second connecting tube 203 installed on the adjusting seat 4 are driven to rotate, thereby further realizing the adjustment of the use angles of the puncture needle 103 and the suction needle 104 at the front end of the first connecting tube 202 and the second connecting tube 203, so as to facilitate more accurate biopsy sampling operations.

[0043] Preferably, the moving assembly includes a slide groove 801 provided on the adjustment seat 4, a slider 802 is slidably connected to the slide groove 801, the mounting seat 6 is fixed to the front side of the slider 802, a second threaded sleeve 803 is fixed to the slider 802, an L-shaped frame 804 is fixed to one side of the adjustment seat 4, a second screw rod 805 is rotatably connected to the L-shaped frame 804, the second screw rod 805 and the second threaded sleeve 803 are engaged with each other, and a fixing pin 806 for driving the second screw rod 805 is fixed to one end of the second screw rod 805;

[0044] It should be noted here that: the second screw rod 805 is driven to rotate through the fixing pin 806. During the rotation of the second screw rod 805, the mutual engagement transmission between the second screw rod 805 and the second threaded sleeve 803 drives the slider 802 to slide on the slide groove 801, and the sliding of the slider 802 drives the mounting seat 6 to move.

[0045] Preferably, the mounting assembly includes a connecting plate 701 fixed on the mounting base 6, and a slot 702 for fixing the first connecting tube 202 or the second connecting tube 203 is provided on the connecting plate 701;

[0046] It should be noted here that the tube bodies of the first connecting tube 202 and the second connecting tube 203 are respectively clamped and installed on the clamping grooves 702 of the two sets of connecting plates 701, so as to facilitate the stable operation and use of the puncture needle 103 and the suction needle 104 at the front end of the first connecting tube 202 and the second connecting tube 203.

[0047] In this solution: a preimplantation genetic diagnosis embryo biopsy device comprises the following steps:

[0048] During the process of performing a biopsy sampling operation on the embryo by the embryo biopsy device before embryo implantation, the ICSI culture dish with the embryo cultured is transferred to the supporting platform 102 of the microscope 101. After placement, the puncture needle 103 and the pipette needle 104 required for the biopsy sampling process are respectively installed on the front ends of the first connecting tube 202 and the second connecting tube 203. After installation, the tube bodies of the first connecting tube 202 and the second connecting tube 203 are respectively clamped and installed on the clamping grooves 702 of the two groups of connecting plates 701. After installation, the use angles of the puncture needle 103 and the pipette needle 104 at the front ends of the first connecting tube 202 and the second connecting tube 203 are adjusted by the rotation of the rotating assembly on the adjustment seat 4, so as to facilitate more accurate biopsy sampling operations.

[0049] After the angle adjustment of the puncture needle 103 and the suction needle 104 is completed, the push plate 1003 is forced to move toward the mounting bracket 201 through the cooperation between the pushing assembly and the guide assembly. During the movement of the push plate 1003, the two groups of piston rods 1002 are pulled outward from the two groups of piston cylinders 1001 respectively. In the process of pulling the piston rods 1002 outward, negative pressure is generated inside the first pressure cylinder 901 and inside the negative pressure chamber of the second pressure cylinder 902. Because the interior of the second pressure cylinder 902 is divided by the piston plate 12, the capacity of the negative pressure chamber inside the second pressure cylinder 902 is smaller than the capacity inside the first pressure cylinder 901, and the two groups of piston rods 1002 slide outward on the two groups of piston cylinders 1001 by the same distance, so that the negative pressure pressure in the negative pressure chamber inside the second pressure cylinder 902 is greater than the pressure inside the first pressure cylinder 901.

[0050] After the negative pressure chambers inside the first pressure cylinder 901 and the second pressure cylinder 902 are evacuated, the end of the pipette needle 104 at the front end of the second connecting tube 203 is pressed against the surface of the embryo inside the ICSI culture dish by the moving component. After the pressing is completed, the second control valve 205 is opened. The interconnected connection of the second connecting tube 203 and the negative pressure of the negative pressure chamber inside the second pressure cylinder 902 are used to press the embryo against the front end of the pipette needle 104. The suction effect on the embryo reduces the swing of the embryo during the insertion of the puncture needle 103, facilitating a more accurate insertion and sampling operation. After the embryo is adsorbed, the end of the puncture needle 103 at the front end of the first connecting tube 202 is inserted into the interior of the embryo by the moving component. After that, the first control valve 204 is opened, and part of the biopsy material inside the embryo is adsorbed to the inside of the puncture needle 103 through the interconnected connection of the first connecting tube 202 and the negative pressure inside the first pressure cylinder 901. At this time, the front end of the puncture needle 103 is removed from the embryo to complete the biopsy sampling operation on the embryo. During the entire sampling process, since the negative pressure of the negative pressure chamber inside the second pressure cylinder 902 is always greater than the pressure inside the first pressure cylinder 901, the interconnected connection between the first connecting tube 202 and the second connecting tube 203 makes the adsorption force of the pipette needle 104 on the embryo greater than the adsorption force of the biopsy material after the puncture needle 103 is inserted, thereby avoiding the embryo swinging and displacement caused by the adsorption pressure on the biopsy material being greater than the pressure on the embryo to be adsorbed and fixed during the adsorption sampling process, and further ensuring the stability of the puncture biopsy sampling process.

[0051] It should be noted that, in this document, relational terms such as first and second, etc., are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the terms "comprises," "comprising," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that includes a list of elements includes not only those elements but also other elements not explicitly listed, or elements inherent to such process, method, article, or apparatus.

[0052] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. A preimplantation genetic diagnosis embryo biopsy device, comprising: A microscope (101) for embryo diagnostic biopsy, wherein the microscope (101) is provided with a support platform (102) for supporting and placing an embryo culture dish, and the microscope (101) is provided with a puncture needle (103) for inserting an embryo sample during embryo biopsy sampling and a pipette needle (104) for adsorbing and fixing the embryo; It is characterized by further comprising: A connecting assembly for connecting a puncture needle (103) and a pipette needle (104) is provided on a microscope (101), wherein the connecting assembly comprises a mounting frame (201) fixed on the microscope (101), a first connecting tube (202) and a second connecting tube (203) are symmetrically mounted on the mounting frame (201), the puncture needle (103) is detachably mounted on the front end of the first connecting tube (202), and the pipette needle (104) is detachably mounted on the front end of the second connecting tube (203), and the first connecting tube (202) and the second connecting tube (203) are respectively provided with a pipe connection. The first control valve (204) and the second control valve (205) are controlled by the valve, and two groups of symmetrically arranged mounting plates (3) are fixed on the mounting frame (201). The front side of the mounting plate (3) is rotatably connected to an adjustment seat (4) through a rotating assembly. The adjustment seat (4) is connected to a mounting seat (6) through a moving assembly. The mounting seat (6) is provided with a mounting assembly for assisting the first connecting tube (202) or the second connecting tube (203) in clamping and installing. The mounting frame (201) is provided with a control assembly for pressure control during the use of the puncture needle (103) and the suction needle (104); The control assembly includes a first pressure cylinder (901) and a second pressure cylinder (902) arranged on the front side of the mounting frame (201), and the aperture and capacity of the first pressure cylinder (901) and the second pressure cylinder (902) are set to be the same, the rear end of the first connecting pipe (202) is connected to the upper end of the first pressure cylinder (901), and the interior of the second pressure cylinder (902) is slidably connected to a piston plate (12) for dividing the internal chamber of the second pressure cylinder (902), and the interior of the second pressure cylinder (902) is movable. The plug plate (12) is divided to form a negative pressure chamber and a normal pressure chamber, the negative pressure chamber is located above the normal pressure chamber, the lower end of the second pressure cylinder (902) is provided with a lifting assembly for adjusting the lifting and lowering of the piston plate (12), the rear end of the second connecting tube (203) is connected to the upper end of the second pressure cylinder (902), and the second connecting tube (203) is connected to the interior of the negative pressure chamber, and the mounting frame (201) is provided with a pressure extraction assembly for extracting pressure from the inside of the first pressure cylinder (901) and the second pressure cylinder (902).

2. The preimplantation genetic diagnosis embryo biopsy device according to claim 1, characterized in that: The lifting assembly includes a first threaded sleeve (1301) fixed to the lower end of the second pressure cylinder (902), and a first screw rod (1302) is threadedly engaged on the first threaded sleeve (1301). One end of the first screw rod (1302) is rotatably connected to the piston plate (12), and the other end of the first screw rod (1302) is fixed with a rotating pin (1303) for driving the first screw rod (1302) to rotate.

3. The preimplantation genetic diagnosis embryo biopsy device according to claim 2, characterized in that: The pumping assembly includes piston cylinders (1001) fixed on the first pressure cylinder (901) and the second pressure cylinder (902), the piston cylinders (1001) are arranged in communication with the interior of the negative pressure chamber, the two groups of piston cylinders (1001) are slidably connected with piston rods (1002), a push plate (1003) is provided on the front side of the mounting frame (201), one end of the two groups of piston rods (1002) is fixed to the push plate (1003), and the mounting frame (201) is provided with a pushing assembly for pushing the push plate (1003) and a guide assembly for guiding during the pushing process.

4. The preimplantation genetic diagnosis embryo biopsy device according to claim 3, characterized in that: The pushing assembly includes a threaded rod (1101) rotatably connected to a mounting frame (201), a threaded tube (1102) being threadedly engaged on the threaded rod (1101), one end of the threaded tube (1102) being fixed to a push plate (1003), and a driving motor (1103) for driving the threaded rod (1101) being installed on the mounting frame (201).

5. The preimplantation genetic diagnosis embryo biopsy device according to claim 4, characterized in that: The guide assembly includes two groups of sleeves (1401) fixed on the push plate (1003), the two groups of sleeves (1401) are symmetrically arranged on both sides of the threaded tube (1102), and the two groups of sleeves (1401) are slidably connected to a sliding rod (1402), one end of the sliding rod (1402) is fixed to the mounting frame (201).

6. The preimplantation genetic diagnosis embryo biopsy device according to claim 1, characterized in that: The rotating assembly comprises a rotating shaft (501) rotatably connected to the mounting plate (3), one end of the rotating shaft (501) is fixed to the back of the adjustment seat (4), an operating box (502) is fixed to the mounting plate (3), the other end of the rotating shaft (501) is located inside the operating box (502) and is fixed with a worm gear (503), an operating shaft (504) is rotatably connected to the operating box (502), a worm (505) is fixed to the operating shaft (504), the worm (505) and the worm gear (503) are meshed with each other, and one end of the operating shaft (504) is located outside the operating box (502) and is fixed with a mounting pin (506).

7. The preimplantation genetic diagnosis embryo biopsy device according to claim 1, characterized in that: The moving assembly comprises a slide groove (801) provided on the adjustment seat (4), a slider (802) being slidably connected to the slide groove (801), the mounting seat (6) being fixed to the front side of the slider (802), a second threaded sleeve (803) being fixed to the slider (802), an L-shaped frame (804) being fixed on one side of the mounting seat (6), a second screw rod (805) being rotatably connected to the L-shaped frame (804), the second screw rod (805) and the second threaded sleeve (803) being meshed with each other, and a fixing pin (806) for driving the second screw rod (805) being fixed to one end of the second screw rod (805).

8. The preimplantation genetic diagnosis embryo biopsy device according to claim 1, characterized in that: The mounting assembly comprises a connecting plate (701) fixed on the mounting seat (6), and a clamping groove (702) for clamping and fixing the first connecting tube (202) or the second connecting tube (203) is provided on the connecting plate (701).

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