A method and tools for establishing a mouse corneal alkali burn model

Abstract

This invention relates to the field of mouse corneal model construction technology, and particularly to a method and tool for establishing a mouse corneal alkali burn model. The invention involves adsorbing an adsorption ring onto the mouse cornea, pumping alkali solution into the adsorption ring to induce alkali burn, determining the negative pressure change curve of the adsorption ring as a function of the alkali solution pumping in, screening characteristic curve segments, determining the adsorption disturbance characterization value, and judging the adsorption state of the adsorption ring. In response to determining that the adsorption state is suspected to be unstable, the invention determines whether the alkali burn of the mouse corneal sample is qualified. For mouse corneal samples with qualified alkali burns, the alkali solution in the adsorption ring is aspirated, thus completing the alkali burn model of the mouse corneal sample. Furthermore, this invention enables the determination of the effectiveness of the mouse corneal burn sample construction based on the difference in the phenomenon of alkali solution introduction affecting the adsorption stability of the adsorption ring, and precise control of the alkali solution's action time, improving the consistency of the mouse corneal alkali burn sample construction.

Description

Technical Field

[0001] This invention relates to the field of mouse corneal model construction technology, and in particular to a method and tool for creating a mouse corneal alkali burn model. Background Technology

[0002] Corneal alkali burns are a common and challenging ocular trauma in clinical practice. Constructing a mouse model of corneal alkali burns can simulate the pathological process of human corneal alkali burns, providing an important experimental basis for studying the pathogenesis, development, and pathological changes of the disease. This helps researchers develop treatment plans and improve treatment outcomes. However, when constructing a mouse model of corneal alkali burns, the negative pressure generated by the adsorption ring fluctuates with the injection of alkali solution due to the elasticity and flexibility of the cornea. This affects the stability of the adhesion between the cornea and the adsorption ring, making it impossible to establish a typical alkali burn model and affecting the effectiveness of the experiment. Therefore, ensuring the effectiveness of constructing a mouse model of corneal alkali burns is a technical problem that urgently needs to be solved.

[0003] For example, Chinese Patent Publication No. CN219148191U discloses a corneal fixation device, which includes an adsorption ring and a lens. The lens is sealed within the adsorption ring, sealing the proximal end of the adsorption ring's inner cavity. An adsorption chamber is formed in the area between the lens and the distal end of the adsorption ring within the adsorption ring's inner cavity. A guide channel is provided on the inner wall of the adsorption chamber, comprising a first channel and multiple second channels. The first channel extends circumferentially within the adsorption chamber, and each of the second channels is distributed circumferentially within the adsorption chamber and intersects and communicates with the first channel. A through channel is formed on the outer peripheral wall of the adsorption ring, communicating with the guide channel. This device can ensure uniform adsorption force on the corneal surface, improving discomfort during the adsorption process.

[0004] The existing technology still has the following problems: it does not consider the impact of different flatness states of mouse corneal samples on the adsorption stability of the adsorption ring, it cannot judge the effectiveness of the construction of mouse corneal burn samples based on the differences in the phenomenon of alkaline solution introduction affecting the adsorption stability of the adsorption ring, and it cannot accurately control the duration of alkaline solution action, which affects the consistency of the construction of mouse corneal alkali burn samples. Summary of the Invention

[0005] Therefore, the present invention provides a method and tool for establishing a mouse corneal alkali burn model, which overcomes the problems in the prior art that the effectiveness of constructing mouse corneal burn samples cannot be judged based on the difference in the phenomenon of alkali introduction affecting the adsorption stability of the adsorption ring, and that the duration of alkali action cannot be precisely controlled.

[0006] To achieve the above objectives, the present invention provides a method for establishing a mouse corneal alkali burn model, comprising:

[0007] The adsorption ring is attached to a predetermined position on a mouse corneal sample, and an alkaline solution is pumped into the adsorption ring to cause alkaline burn.

[0008] In response to receiving a trigger signal that the micro-pump has started pumping alkaline solution into the adsorption ring, the negative pressure value of the adsorption ring is determined as a negative pressure change curve as the amount of alkaline solution pumped in changes.

[0009] The negative pressure change curve is divided into several curve segments at preset alkali pumping volume intervals. Characterization curve segments are screened based on the alkali pumping volume. Adsorption disturbance characterization values ​​are determined according to the negative pressure extreme values ​​of each characterization curve segment to determine whether the adsorption state of the adsorption ring is a suspected unstable state.

[0010] In response to determining that the adsorption state of the adsorption ring is a suspected unstable state, the adsorption disturbance factor of each curve segment is determined according to the amount of alkali pumped in corresponding to the negative pressure extreme data point of each curve segment, so as to determine whether the alkali burn of the mouse corneal sample is qualified.

[0011] Retain qualified mouse corneal samples with alkali burns, and at a preset alkali burn cutoff time, aspirate the alkali solution in the adsorption ring to complete the alkali burn modeling of mouse corneal samples.

[0012] The alkali burn cutoff time is a time after a preset duration based on the time corresponding to the trigger signal.

[0013] Furthermore, the process of determining the negative pressure change curve includes:

[0014] Obtain the alkali pumping rate and the negative pressure value of the adsorption ring at several time points. Establish a rectangular coordinate system with the negative pressure value as the vertical axis and the alkali pumping rate as the horizontal axis. Plot the curve of the negative pressure value changing with the alkali pumping rate and determine the curve as the negative pressure change curve.

[0015] Furthermore, the process of selecting the characteristic curve segment includes:

[0016] The alkali solution pumping rate at several time points is obtained, and the curve segment containing the maximum and minimum alkali solution pumping rate is determined as the characterization curve segment, wherein,

[0017] The curve segment containing the minimum amount of alkaline solution pumped into the adsorption ring is defined as the first characterization curve segment.

[0018] The curve segment containing the maximum amount of alkali solution pumped into the adsorption ring is defined as the second characterization curve segment.

[0019] Furthermore, the process of determining the adsorption perturbation characterization values ​​includes:

[0020] Obtain the negative pressure values ​​corresponding to the negative pressure extreme value data points of the first characterization curve segment and the negative pressure values ​​corresponding to the negative pressure extreme value data points of the second characterization curve segment;

[0021] The negative pressure extreme value data points include negative pressure maximum value data points and negative pressure minimum value data points;

[0022] The difference between the negative pressure value corresponding to the negative pressure maximum data point and the negative pressure value corresponding to the negative pressure minimum data point in the first characterization curve segment is determined as the first negative pressure difference value, and the difference between the negative pressure value corresponding to the negative pressure maximum data point and the negative pressure value corresponding to the negative pressure minimum data point in the second characterization curve segment is determined as the second negative pressure difference value.

[0023] The absolute value of the difference between the first negative pressure difference and the second negative pressure difference is determined as the adsorption disturbance characterization value.

[0024] Furthermore, the process of determining whether the adsorption state of the adsorption ring is a suspected unstable state includes:

[0025] The adsorption disturbance characterization value is compared with a preset adsorption disturbance characterization value threshold. If the adsorption disturbance characterization value is greater than the adsorption disturbance characterization value threshold, the adsorption state of the adsorption ring is determined to be a suspected unstable state.

[0026] Furthermore, the process of determining the alkali pumping rate corresponding to the negative pressure extreme data points of each curve segment includes:

[0027] Determine the maximum and minimum negative pressure data points for each curve segment, and obtain the alkali pumping rate corresponding to the maximum and minimum negative pressure data points.

[0028] Furthermore, the process of calculating the adsorption perturbation factor for each curve segment includes:

[0029] Calculate the absolute value of the difference between the alkali pumping rate corresponding to the negative pressure maximum data point and the alkali pumping rate corresponding to the negative pressure minimum data point in each curve segment.

[0030] The ratio of the absolute value of the difference to the preset numerical interval of the alkali solution pumping amount is determined as the adsorption disturbance factor of the curve segment.

[0031] Furthermore, the process of determining whether the alkali burn of the mouse corneal sample is acceptable includes:

[0032] Calculate the average value of the adsorption perturbation factor for each curve segment;

[0033] The average value is compared with the adsorption perturbation factor threshold. If the average value of the adsorption perturbation factor is less than the adsorption perturbation factor threshold, the alkali burn of the mouse corneal sample is deemed acceptable.

[0034] Furthermore, the present invention also provides a tool for creating a mouse corneal alkali burn model, comprising:

[0035] An adsorption ring includes a ring body, a number of negative pressure holes at the bottom of the ring body for adsorbing mouse cornea, an inlet hole for introducing alkaline solution into mouse cornea, and an outlet hole for discharging alkaline solution into mouse cornea.

[0036] A micro-pump, which is connected to the ring body through a connecting pipe, is used to pump alkaline solution into the adsorption ring;

[0037] An aspirator, which is connected to the ring body through an aspirator tube, is used to draw out the alkaline solution inside the adsorption ring;

[0038] A timer, which is connected to the micro-pump and the aspirator respectively, is used to take the time corresponding to the trigger signal issued by the micro-pump as the reference time, and to determine the alkali burn cutoff time after a preset duration based on the reference time.

[0039] Furthermore, the adsorption ring also includes a negative pressure conducting cavity, a first guiding cavity, and a second guiding cavity. One end of the negative pressure conducting cavity is connected to a negative pressure hole, and the other end is connected to a negative pressure pump for generating a preset negative pressure value through a gas guiding pipe. The gas guiding pipe is also equipped with a negative pressure monitoring instrument for obtaining the negative pressure value of the adsorption ring and plotting the negative pressure change curve.

[0040] The first flow channel is used to connect the connecting tube to the inlet hole, and the second flow channel is used to connect the suction tube to the outlet hole;

[0041] The negative pressure monitor is also connected to a data filtering unit and an analysis unit. The data filtering unit is used to filter characterization curve segments and determine adsorption disturbance characterization values. Based on the adsorption disturbance characterization values, it is determined whether the adsorption state of the adsorption ring is a suspected unstable state.

[0042] The analysis unit is used to determine the adsorption perturbation factor of each curve segment in order to determine whether the alkali burn of the mouse corneal sample is acceptable.

[0043] Compared with the prior art, the beneficial effects of the present invention are as follows: The present invention adsorbs an adsorption ring at a predetermined position on a mouse corneal sample, pumps alkaline solution into the adsorption ring to induce alkali burn, determines the negative pressure change curve of the adsorption ring as a function of the amount of alkali solution pumped in, divides the negative pressure change curve into several curve segments, screens the characteristic curve segments, determines the adsorption disturbance characterization value, and judges the adsorption state of the adsorption ring. In response to determining that the adsorption state is a suspected unstable state, it is determined whether the alkali burn of the mouse corneal sample is qualified. The mouse corneal samples with qualified alkali burns are retained, and the alkaline solution in the adsorption ring is aspirated at the alkali burn cutoff time, thus completing the alkali burn modeling of the mouse corneal sample. In this way, the effectiveness of the mouse corneal burn sample construction can be judged based on the difference in the phenomenon of the influence of alkali introduction on the adsorption stability of the adsorption ring, and the duration of alkali action can be precisely controlled, thereby improving the consistency of the mouse corneal alkali burn sample construction.

[0044] In particular, this invention selects characterization curve segments based on the amount of alkali pumped into each curve segment. As those skilled in the art will understand, the cornea has a certain degree of elasticity and flexibility. The pumping in of alkali will quickly break the original negative pressure state. The elasticity and flexibility of the cornea cannot respond sufficiently in the initial stage of alkali injection, which easily leads to unstable pressure distribution. Under uneven pressure, the cornea is prone to deformation and wrinkles, affecting the stability of the adhesion between the cornea and the adsorption ring. When the amount of alkali pumped into the curve reaches its maximum value, the gravitational factor of the pumped alkali will affect the elasticity of the corneal surface, making the adhesion between the cornea and the adsorption ring unstable. This invention selects the curve segments containing the minimum and maximum values ​​of the alkali pumped into the characterization curve segments, thereby realizing the acquisition of characterization data based on the influence of alkali introduction on the adsorption stability of the adsorption ring.

[0045] In particular, this invention determines the adsorption perturbation characterization value by the absolute value of the difference between the negative pressure extreme values ​​of each characterization curve segment. Those skilled in the art will understand that by determining the negative pressure extreme points of the characterization curve segment, the upper and lower boundaries of the negative pressure fluctuation within the characterization curve segment can be clearly defined, and the stability and changes of the negative pressure within the curve segment can be intuitively understood. The difference between the maximum and minimum values ​​of the negative pressure in the first characterization curve segment characterizes the degree of adsorption state fluctuation of the adsorption ring affected by the introduction of alkali solution in the initial stage of alkali solution pumping. The difference between the maximum and minimum values ​​of the negative pressure in the second characterization curve segment characterizes the degree of adsorption state fluctuation of the adsorption ring affected by the introduction of alkali solution in the final stage of alkali solution pumping. By comparing the negative pressure difference between the first and second characterization curve segments, the differences in the adsorption stability of the adsorption ring at different stages of alkali solution pumping are characterized. The greater the difference in the negative pressure difference, the worse the adsorption stability of the adsorption ring is during the overall alkali solution pumping process. Thus, the degree of adsorption state fluctuation of the adsorption ring affected by the introduction of alkali solution during alkali solution pumping is quantified, improving the consistency of mouse corneal alkali burn samples.

[0046] In particular, this invention calculates the adsorption disturbance factor for each curve segment by the ratio of the absolute value of the difference in alkali injection volume corresponding to the extreme negative pressure data points of each curve segment to the preset numerical interval of the alkali injection volume. Those skilled in the art will understand that the cornea has a certain degree of elasticity and flexibility. During the alkali injection process, the negative pressure value of the adsorption ring will fluctuate with the alkali injection. The elasticity and flexibility of the cornea can quickly stabilize the negative pressure fluctuation. The smaller the ratio of the absolute value of the difference in negative pressure extreme values ​​of the curve segment to the preset numerical interval of the alkali injection volume, the more stable the negative pressure fluctuation of the adsorption ring tends to be. The faster the speed, the better the adsorption stability. The larger the ratio of the absolute value of the difference in alkali pumping volume corresponding to the extreme negative pressure data points of the curve segment to the preset numerical interval of the alkali pumping volume, the more it indicates that the negative pressure fluctuation within the curve segment shows an overall upward or downward trend, and the worse the adsorption stability of the adsorption ring. This invention calculates the adsorption disturbance factor of each curve segment by the ratio of the absolute value of the difference in alkali pumping volume corresponding to the extreme negative pressure data points of each curve segment to the preset numerical interval of the alkali pumping volume. Thus, it realizes the quantification of the negative pressure fluctuation within the curve segment and improves the consistency of the mouse corneal alkali burn sample construction.

[0047] In particular, this invention determines whether the alkali burn of mouse corneal samples is qualified by calculating the average value of the adsorption perturbation factor of each curve segment. The adsorption perturbation factor corresponding to each curve segment can characterize the adsorption state of the adsorption ring. The average value of the adsorption perturbation factor characterizes the fluctuation of the adsorption state of the adsorption ring during the alkali burn process. The larger the average value of the adsorption perturbation factor, the more obvious the unstable adsorption phenomenon of the adsorption ring. As those skilled in the art can understand, the unstable adsorption state of the adsorption ring will cause the alkali solution to seep out of the adsorption ring, resulting in an uncontrollable distribution area of ​​the alkali solution on the surface of the mouse cornea, affecting the consistency of model construction of mouse corneal alkali burn samples. This invention realizes the determination of whether the alkali burn of mouse corneal samples is qualified by evaluating the adsorption instability state of the adsorption ring, and improves the consistency of mouse corneal alkali burn sample construction. Attached Figure Description

[0048] Figure 1 This is a step diagram of the mouse corneal alkali burn modeling method according to an embodiment of the present invention;

[0049] Figure 2 A flowchart illustrating the steps for determining adsorption perturbation characterization values ​​in an embodiment of the present invention;

[0050] Figure 3 This is a simplified structural diagram of the adsorption ring in an embodiment of the present invention;

[0051] Figure 4 This is a structural block diagram of the mouse corneal alkali burn modeling tool according to an embodiment of the present invention;

[0052] In the figure: First guide cavity 1, inlet hole 2, negative pressure conduction cavity 3, negative pressure hole 4, second guide cavity 5, outlet hole 6, connecting pipe 7, air guide pipe 8, suction pipe 9, ring body 10. Detailed Implementation

[0053] To make the objectives and advantages of the present invention clearer, the present invention will be further described below with reference to embodiments; it should be understood that the specific embodiments described herein are merely for explaining the present invention and are not intended to limit the present invention.

[0054] Preferred embodiments of the present invention will now be described with reference to the accompanying drawings. Those skilled in the art should understand that these embodiments are merely illustrative of the technical principles of the present invention and are not intended to limit the scope of protection of the present invention.

[0055] It should be noted that in the description of this invention, the terms "upper," "lower," "inner," "outer," etc., which indicate the direction or positional relationship, are based on the direction or positional relationship shown in the drawings. This is only for the convenience of description and is not intended to indicate or imply that the device or element must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, it should not be construed as a limitation of this invention.

[0056] Furthermore, it should be noted that, in the description of this invention, unless otherwise explicitly specified and limited, the terms "installation" and "connection" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0057] Please see Figure 1 The diagram illustrates the steps of the mouse corneal alkali burn modeling method of the present invention. The mouse corneal alkali burn modeling method of the present invention includes:

[0058] Step S100: Adsorb the adsorption ring onto the predetermined position of the mouse corneal sample, and pump alkaline solution into the adsorption ring to perform alkaline burn.

[0059] Step S200: In response to receiving a trigger signal that the micro-pump has started pumping alkaline solution into the adsorption ring, determine the negative pressure change curve of the adsorption ring as the amount of alkaline solution pumped in.

[0060] Step S300: Divide the negative pressure change curve into several curve segments at preset alkali pumping volume intervals, screen characterization curve segments based on the alkali pumping volume, and determine the adsorption disturbance characterization value according to the negative pressure extreme value of each characterization curve segment, so as to determine whether the adsorption state of the adsorption ring is a suspected unstable state.

[0061] Step S400: In response to determining that the adsorption state of the adsorption ring is a suspected unstable state, the adsorption disturbance factor of each curve segment is determined according to the amount of alkali pumped in according to the negative pressure extreme value data point of each curve segment, so as to determine whether the alkali burn of the mouse corneal sample is qualified.

[0062] Step S500: Retain the qualified mouse corneal samples with alkali burn, and at the preset alkali burn cutoff time, aspirate the alkali solution in the adsorption ring to complete the alkali burn modeling of the mouse corneal samples.

[0063] The alkali burn cutoff time is a time after a preset duration based on the time corresponding to the trigger signal.

[0064] Specifically, the alkaline solution in this invention can be a sodium hydroxide solution with a concentration of 1N, meaning that 1L of sodium hydroxide solution contains 40g of sodium hydroxide.

[0065] Specifically, the predetermined position where the adsorption ring adheres to the mouse corneal sample can be set by those skilled in the art according to the modeling requirements of the mouse corneal alkali burn sample. Preferably, the predetermined position where the adsorption ring adheres to the mouse corneal sample can be the central position of the mouse cornea. The structure of the central region of the mouse cornea is relatively uniform and the thickness is relatively consistent. When performing alkali burn modeling, placing the adsorption ring in the central position of the cornea can ensure that the alkali solution has a relatively uniform effect on the cornea, and can make the constructed mouse corneal alkali burn model more representative.

[0066] Specifically, the preset interval of the alkali infusion volume for dividing the curve segment can be set by those skilled in the art based on the degree of alkali burn in the constructed mouse corneal alkali burn model. The larger the required alkali infusion volume, the larger the preset interval. Preferably, the preset interval can be 1 μL.

[0067] Specifically, the preset duration between the preset alkali burn cutoff time and the reference time corresponding to the trigger signal can be set by those skilled in the art based on the degree of alkali burn in the constructed mouse corneal alkali burn model. Preferably, the preset alkali burn duration can be in the range of [30, 80], with the interval unit being seconds.

[0068] Specifically, the process of determining the negative pressure change curve includes:

[0069] Obtain the alkali pumping rate and the negative pressure value of the adsorption ring at several time points. Establish a rectangular coordinate system with the negative pressure value as the vertical axis and the alkali pumping rate as the horizontal axis. Plot the curve of the negative pressure value changing with the alkali pumping rate and determine the curve as the negative pressure change curve.

[0070] Specifically, the alkali pumping rate q is calculated based on the flow rate f of the micro pump and the working time t of the micro pump monitored by the timer, where q = f × t.

[0071] Specifically, the process of selecting the characteristic curve segment includes:

[0072] The alkali solution pumping rate at several time points is obtained, and the curve segment containing the maximum and minimum alkali solution pumping rate is determined as the characterization curve segment, wherein,

[0073] The curve segment containing the minimum amount of alkaline solution pumped into the adsorption ring is defined as the first characterization curve segment.

[0074] The curve segment containing the maximum amount of alkali solution pumped into the adsorption ring is defined as the second characterization curve segment.

[0075] Specifically, this invention selects characterization curve segments based on the amount of alkali pumped into each curve segment. Those skilled in the art will understand that the cornea possesses a certain degree of elasticity and flexibility. The pumping in of alkali quickly disrupts the original negative pressure state. The cornea's elasticity and flexibility cannot fully respond in the initial stage of alkali injection, easily leading to unstable pressure distribution. Under uneven pressure, the cornea is prone to deformation and wrinkling, affecting the stability of the adhesion between the cornea and the adsorption ring. When the alkali pumping inflow reaches its maximum value, the gravitational factor of the pumped alkali affects the elasticity of the corneal surface, making the adhesion between the cornea and the adsorption ring unstable. This invention selects the curve segments containing the minimum and maximum values ​​of the alkali pumping inflow as characterization curve segments, thereby achieving the acquisition of characterization data based on the impact of alkali introduction on the adsorption stability of the adsorption ring.

[0076] Specifically, please refer to Figure 2 This is a flowchart illustrating the steps for determining the adsorption perturbation characterization value in an embodiment of the present invention. The process for determining the adsorption perturbation characterization value includes:

[0077] Step S301: Obtain the negative pressure value corresponding to the negative pressure extreme value data point of the first characterization curve segment and the negative pressure value corresponding to the negative pressure extreme value data point of the second characterization curve segment.

[0078] The negative pressure extreme value data points include negative pressure maximum value data points and negative pressure minimum value data points;

[0079] Step S302: The difference between the negative pressure value corresponding to the negative pressure maximum value data point and the negative pressure value corresponding to the negative pressure minimum value data point of the first characterization curve segment is determined as the first negative pressure difference C1, and the difference between the negative pressure value corresponding to the negative pressure maximum value data point and the negative pressure value corresponding to the negative pressure minimum value data point of the second characterization curve segment is determined as the second negative pressure difference C2.

[0080] Step S303: The absolute value of the difference between the first negative pressure difference C1 and the second negative pressure difference C2 is determined as the adsorption disturbance characterization value C, where C = |C1-C2|.

[0081] Specifically, this invention determines the adsorption perturbation characterization value by the absolute value of the difference between the negative pressure extreme values ​​of each characterization curve segment. Those skilled in the art will understand that by determining the negative pressure extreme points of the characterization curve segment, the upper and lower boundaries of the negative pressure fluctuation within the segment can be clearly defined, providing an intuitive understanding of the stability and changes in negative pressure within the segment. The difference between the maximum and minimum negative pressure values ​​of the first characterization curve segment characterizes the degree of adsorption state fluctuation of the adsorption ring under the influence of alkali introduction during the initial stage of alkali pumping. The difference between the maximum and minimum negative pressure values ​​of the second characterization curve segment characterizes the degree of adsorption state fluctuation of the adsorption ring under the influence of alkali introduction during the final stage of alkali pumping. By comparing the negative pressure difference between the first and second characterization curve segments, the differences in adsorption stability of the adsorption ring at different stages of alkali pumping are characterized. The greater the difference in negative pressure difference, the worse the adsorption stability of the adsorption ring during the overall alkali pumping process. Thus, the degree of adsorption state fluctuation of the adsorption ring under the influence of alkali introduction during alkali pumping is quantified, improving the consistency of mouse corneal alkali burn samples.

[0082] Specifically, the process of determining whether the adsorption state of the adsorption ring is a suspected unstable state includes:

[0083] The adsorption disturbance characterization value C is compared with the preset adsorption disturbance characterization value threshold C0. If the adsorption disturbance characterization value C is greater than the adsorption disturbance characterization value threshold C0, the adsorption state of the adsorption ring is determined to be a suspected unstable state.

[0084] If the adsorption disturbance characterization value C is less than or equal to the adsorption disturbance characterization value threshold C0, then the adsorption state of the adsorption ring is determined to be a stable state.

[0085] Specifically, the preset adsorption perturbation characterization value threshold C0 can be set by those skilled in the art based on the consistency requirements of constructing mouse corneal alkali burn samples. The higher the consistency requirement, the smaller the set adsorption perturbation characterization value threshold C0. Preferably, the adsorption perturbation characterization value threshold C0 can be [0.08, 0.12], with the unit of Pa.

[0086] Specifically, the process of determining the alkali pumping rate corresponding to the negative pressure extreme data points of each curve segment includes:

[0087] Determine the maximum and minimum negative pressure data points for each curve segment, and obtain the alkali pumping rate corresponding to the maximum and minimum negative pressure data points.

[0088] Specifically, the process of calculating the adsorption perturbation factor for each curve segment includes:

[0089] Calculate the alkali pumping rate q corresponding to the maximum negative pressure data point for each curve segment. max The alkali pumping rate q corresponding to the minimum negative pressure data point min The absolute value of the difference, P, where P = |q max -q min |;

[0090] The ratio of the absolute value of the difference P to the preset numerical interval Q of the alkali pumping amount is determined as the adsorption disturbance factor Y of the curve segment, where Y = P / Q.

[0091] Specifically, this invention calculates the adsorption disturbance factor for each curve segment by the ratio of the absolute value of the difference in alkali injection volume corresponding to the extreme negative pressure data points of each curve segment to the preset numerical interval of the alkali injection volume. Those skilled in the art will understand that the cornea has a certain degree of elasticity and flexibility. During the alkali injection process, the negative pressure value of the adsorption ring will fluctuate with the alkali injection. The elasticity and flexibility of the cornea can quickly stabilize the negative pressure fluctuation. The smaller the ratio of the absolute value of the difference in negative pressure extreme values ​​of the curve segment to the preset numerical interval of the alkali injection volume, the more stable the negative pressure fluctuation of the adsorption ring tends to be. The faster the speed, the better the adsorption stability. The larger the ratio of the absolute value of the difference in alkali pumping volume corresponding to the extreme negative pressure data points of each curve segment to the preset numerical interval of the alkali pumping volume, the more it indicates that the negative pressure fluctuation within the curve segment shows an overall upward or downward trend, and the worse the adsorption stability of the adsorption ring. This invention calculates the adsorption disturbance factor of each curve segment by the ratio of the absolute value of the difference in alkali pumping volume corresponding to the extreme negative pressure data points of each curve segment to the preset numerical interval of the alkali pumping volume. Thus, it realizes the quantification of the negative pressure fluctuation within the curve segment and improves the consistency of the mouse corneal alkali burn sample construction.

[0092] Specifically, the process for determining whether the alkali burn of the mouse corneal sample is acceptable includes:

[0093] Calculate the average value Y' of the adsorption perturbation factor for each curve segment. , where n is the number of curve segments, Yi is the adsorption perturbation factor of the i-th curve segment, and i is 1, 2, 3...n.

[0094] The average value Y' is compared with the adsorption perturbation factor threshold Y0. If the average value Y' of the adsorption perturbation factor is less than the adsorption perturbation factor threshold Y0, the alkali burn of the mouse corneal sample is deemed qualified.

[0095] If the average value of the adsorption perturbation factor Y' is greater than or equal to the adsorption perturbation factor threshold Y0, then the alkali burn of the mouse corneal sample is deemed unqualified.

[0096] Specifically, the value of the adsorption perturbation factor threshold Y0 can be set by those skilled in the art based on the consistency requirements of the mouse corneal alkali burn model. Preferably, the value of the adsorption perturbation factor threshold Y0 can be 0.7. The higher the consistency requirement, the smaller the value of the adsorption perturbation factor threshold Y0.

[0097] Specifically, this invention determines whether alkali burns in mouse corneal samples are acceptable by calculating the average value of the adsorption perturbation factor for each curve segment. The adsorption perturbation factor corresponding to each curve segment can characterize the adsorption state of the adsorption ring. The average value of the adsorption perturbation factor characterizes the fluctuation of the adsorption state of the adsorption ring during alkali burn. The larger the average value of the adsorption perturbation factor, the more obvious the unstable adsorption phenomenon of the adsorption ring. As those skilled in the art can understand, the unstable adsorption state of the adsorption ring will cause the alkali solution to seep out of the adsorption ring, resulting in an uncontrollable distribution area of ​​the alkali solution on the surface of the mouse cornea, affecting the consistency of model construction for mouse corneal alkali burn samples. This invention achieves the determination of whether alkali burns in mouse corneal samples are acceptable by evaluating the adsorption instability state of the adsorption ring, thereby improving the consistency of mouse corneal alkali burn sample construction.

[0098] Specifically, please refer to Figure 3 as well as Figure 4 As shown, Figure 3 This is a simplified structural diagram of the adsorption ring in an embodiment of the present invention. Figure 4 This is a structural block diagram of a mouse corneal alkali burn modeling tool according to an embodiment of the present invention. The present invention also provides a mouse corneal alkali burn modeling tool, comprising:

[0099] The adsorption ring includes a ring body 10, a plurality of negative pressure holes 4 for adsorbing mouse cornea, an inlet hole 2 for introducing alkaline solution into mouse cornea, and an outlet hole 6 for discharging alkaline solution into mouse cornea.

[0100] A micro-pump, which is connected to the ring body through a connecting pipe 7, is used to pump alkaline solution into the adsorption ring;

[0101] An aspirator, which is connected to the ring body through an aspirator tube 9, is used to draw out the alkaline solution inside the adsorption ring;

[0102] A timer, which is connected to the micro-pump and the aspirator respectively, is used to take the time corresponding to the trigger signal issued by the micro-pump as the reference time, and to determine the alkali burn cutoff time after a preset duration based on the reference time.

[0103] Specifically, the present invention does not limit the specific structure of the micro-pump, which is a common device in the medical field and will not be described in detail here.

[0104] Specifically, the present invention does not limit the specific structure of the suction device. Preferably, it can be composed of a vacuum pump and a collection container, which is prior art and will not be described in detail here.

[0105] Specifically, the present invention does not limit the specific structure of the timer. Preferably, it can be a timer with a positive timing mode, used to time based on the time corresponding to the trigger signal issued by the micro pump as the reference time. The timer is existing technology and will not be described in detail here.

[0106] For details, please continue reading Figure 3 as well as Figure 4 The adsorption ring also includes a negative pressure conduction cavity 3, a first flow guide cavity 1 and a second flow guide cavity 5. One end of the negative pressure conduction cavity 3 is connected to a negative pressure hole 4, and the other end is connected to a negative pressure pump to generate a preset negative pressure value through a gas guide pipe 8. A negative pressure monitor is also provided on the gas guide pipe 8 to obtain the negative pressure value of the adsorption ring and to plot the negative pressure change curve.

[0107] The first guide cavity 1 is used to connect the connecting tube 7 to the inlet hole 2, and the second guide cavity 5 is used to connect the suction tube 9 to the outlet hole 6;

[0108] The negative pressure monitor is also connected to a data filtering unit and an analysis unit. The data filtering unit is used to filter characterization curve segments and determine adsorption disturbance characterization values. Based on the adsorption disturbance characterization values, it is determined whether the adsorption state of the adsorption ring is a suspected unstable state.

[0109] The analysis unit is used to determine the adsorption perturbation factor of each curve segment in order to determine whether the alkali burn of the mouse corneal sample is acceptable.

[0110] Specifically, the present invention does not limit the specific structure of the negative pressure pump, which is a common device in the medical field and will not be described in detail here.

[0111] Specifically, the present invention does not limit the specific structure of the negative pressure monitoring device. Preferably, it can be a digital pressure gauge that senses pressure changes through a built-in high-precision pressure sensor, converts the pressure signal into an electrical signal, and displays the current negative pressure value of the adsorption ring in real time. This is prior art and will not be described in detail here.

[0112] The technical solution of the present invention has been described above with reference to the preferred embodiments shown in the accompanying drawings. However, it will be readily understood by those skilled in the art that the scope of protection of the present invention is obviously not limited to these specific embodiments. Without departing from the principles of the present invention, those skilled in the art can make equivalent changes or substitutions to the relevant technical features, and the technical solutions after these changes or substitutions will all fall within the scope of protection of the present invention.

[0113] The above description is merely a preferred embodiment of the present invention and is not intended to limit the invention. Various modifications and variations can be made to the present invention by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A method for modeling an alkali burn of a mouse cornea, characterized by, The method comprises the following steps: adsorbing the adsorption ring at a predetermined position of the mouse corneal sample, pumping the alkali solution into the adsorption ring to cause alkali burn; in response to receiving a trigger signal for starting pumping the alkali solution into the adsorption ring by the micro pump, determining a negative pressure change curve of the adsorption ring with the change of the amount of pumped alkali solution; dividing the negative pressure change curve into a plurality of curve segments at preset numerical intervals of the amount of pumped alkali solution, screening a curve segment represented by the amount of pumped alkali solution, determining an adsorption disturbance representation value according to the negative pressure extreme value of each curve segment to determine whether the adsorption state of the adsorption ring is a suspected unstable state; in response to determining that the adsorption state of the adsorption ring is a suspected unstable state, determining an adsorption disturbance factor of each curve segment according to the amount of pumped alkali solution corresponding to the negative pressure extreme value data point of each curve segment to determine whether the alkali burn of the mouse corneal sample is qualified; retaining the mouse corneal sample with qualified alkali burn, pumping out the alkali solution in the adsorption ring at a preset alkali burn cutoff time to complete the modeling of the mouse corneal sample with alkali burn; wherein the alkali burn cutoff time is a time after a preset time length from a reference time corresponding to the trigger signal; wherein the process of screening the curve segment represented includes: obtaining the amount of pumped alkali solution at a plurality of times, and determining the curve segment where the maximum value of the amount of pumped alkali solution as the curve segment represented, wherein the curve segment where the minimum value of the amount of pumped alkali solution in the adsorption ring is determined as the first curve segment represented, and the curve segment where the maximum value of the amount of pumped alkali solution in the adsorption ring is determined as the second curve segment represented; the process of determining the adsorption disturbance representation value includes: obtaining the negative pressure value corresponding to the negative pressure extreme value data point of the first curve segment represented and the negative pressure value corresponding to the negative pressure extreme value data point of the second curve segment represented; wherein the negative pressure extreme value data point includes a negative pressure maximum value data point and a negative pressure minimum value data point; determining the difference between the negative pressure value corresponding to the negative pressure maximum value data point and the negative pressure value corresponding to the negative pressure minimum value data point of the first curve segment represented as a first negative pressure difference, and determining the difference between the negative pressure value corresponding to the negative pressure maximum value data point and the negative pressure value corresponding to the negative pressure minimum value data point of the second curve segment represented as a second negative pressure difference; determining the absolute value of the difference between the first negative pressure difference and the second negative pressure difference as the adsorption disturbance representation value; the process of determining the amount of pumped alkali solution corresponding to the negative pressure extreme value data point of each curve segment includes: determining the negative pressure maximum value data point and the negative pressure minimum value data point of each curve segment, and obtaining the amount of pumped alkali solution corresponding to the negative pressure maximum value data point and the amount of pumped alkali solution corresponding to the negative pressure minimum value data point; the process of calculating the adsorption disturbance factor of each curve segment includes: calculating the absolute value of the difference between the amount of pumped alkali solution corresponding to the negative pressure maximum value data point and the amount of pumped alkali solution corresponding to the negative pressure minimum value data point of each curve segment; and determining the ratio of the absolute value to the preset numerical interval of the amount of pumped alkali solution as the adsorption disturbance factor of the curve segment.

2. The mouse corneal alkali burn modeling method according to claim 1, characterized by, the process of determining the negative pressure change curve includes: Obtain the alkali pump-in amount and the negative pressure value of the adsorption ring at several time points, establish a rectangular coordinate system with the negative pressure value as the vertical axis and the alkali pump-in amount as the horizontal axis, draw a curve of the negative pressure value changing with the alkali pump-in amount, and determine the curve as the negative pressure change curve.

3. The mouse corneal alkali burn modeling method according to claim 2, characterized by, The process of determining whether the adsorption state of the adsorption ring is a suspected unstable state includes: Comparing the adsorption disturbance characteristic value with a preset adsorption disturbance characteristic value threshold, and if the adsorption disturbance characteristic value is greater than the adsorption disturbance characteristic value threshold, determining that the adsorption state of the adsorption ring is a suspected unstable state.

4. The mouse corneal alkali burn modeling method according to claim 3, characterized by, The process of determining whether the alkali burn of the mouse cornea sample is qualified includes: Calculating the average value of the adsorption disturbance factor of each curve segment; Comparing the average value with an adsorption disturbance factor threshold, and if the adsorption disturbance factor average value is less than the adsorption disturbance factor threshold, determining that the alkali burn of the mouse cornea sample is qualified.

5. A mouse corneal alkali burn modeling tool for performing the mouse corneal alkali burn modeling method of any one of claims 1-4, wherein, It includes: An adsorption ring including a ring body, a plurality of negative pressure holes arranged at the bottom of the ring body for adsorbing mouse corneas, an introduction hole for introducing alkali into the mouse corneas, and an export hole for exporting alkali from the mouse corneas; A micro pump connected to the ring body through a connecting pipe for pumping alkali into the adsorption ring; A suction device connected to the ring body through a suction pipe for sucking out the alkali in the adsorption ring; A timer connected to the micro pump and the suction device for taking the time corresponding to the trigger signal sent by the micro pump as the reference time, and determining the alkali burn cutoff time after a preset time period based on the reference time; The adsorption ring further includes a negative pressure conduction cavity, a first flow guide cavity, and a second flow guide cavity. One end of the negative pressure conduction cavity is connected to the negative pressure hole, and the other end is connected to a negative pressure pump for generating a preset negative pressure value through a gas guide pipe. A negative pressure monitor for obtaining the negative pressure value of the adsorption ring is also arranged on the gas guide pipe; The first flow guide cavity is used to connect the connecting pipe and the introduction hole, and the second flow guide cavity is used to connect the suction pipe and the export hole; The negative pressure monitor is also connected to a data screening unit and an analysis unit. The data screening unit is used to screen the characteristic curve segments and determine the adsorption disturbance characteristic value, and determine whether the adsorption state of the adsorption ring is a suspected unstable state according to the adsorption disturbance characteristic value; The analysis unit is used to determine the adsorption disturbance factor of each curve segment to determine whether the alkali burn of the mouse cornea sample is qualified.

Images