Polygonatum sibiricum extract and application thereof in preparation of functional alcoholic beverage

Abstract

The invention discloses a rhizoma polygonati extract and application thereof in preparation of a functional alcoholic beverage, and belongs to the technical field of functional alcoholic beverages. The prepared rhizoma polygonati extract can be mixed and dissolved with beer and white spirit, and the rise of uric acid caused by drinking a large amount of beer can be remarkably reduced by drinking the beer added with the prepared rhizoma polygonati extract; the increase of AST and ALT caused by drinking a large amount of white spirit can be remarkably reduced by drinking the white spirit added with the rhizoma polygonati extract prepared by the invention, and the rhizoma polygonati extract can be used for developing beer with the function of reducing uric acid and white spirit with the function of preventing acute alcoholic liver injury; the provided rhizoma polygonati extract does not affect the appearance quality and taste of beer and white spirit, the preparation method is simple and easy to operate, special equipment is not needed, high temperature and high pressure are not needed, the preparation process is safe, toxic and harmful substances are not generated, energy is saved, environment friendliness is achieved, and the method is suitable for large-scale production.

Description

Technical Field

[0001] The present invention relates to the technical field of functional alcoholic beverages, and particularly to a polygonatum sibiricum extract and its application in the preparation of functional alcoholic beverages. Background Art

[0002] Alcoholic beverages refer to beverages for people to drink with an ethanol content of more than 0.5% (vol), including various types of fermented wines, distilled spirits, compounded wines, and pre-mixed cocktails, etc. Beer and liquor are two of the most widely consumed alcoholic beverages. Beer is a fermented wine fermented from malt, water, hops, and yeast, with an alcohol content generally between 3% and 10%. It has a relatively light taste and usually has a refreshing bitterness and a faint malt aroma. Liquor is a distilled spirit made from sorghum, corn, wheat, etc. through processes such as fermentation, distillation, and aging. The fermented liquor is heated and distilled to increase the alcohol concentration. It has a relatively high alcohol content, generally between 38% and 68%, and has a strong and pungent taste.

[0004] However, excessive drinking can cause many damages to the human body, mainly including the following aspects: 1) Liver damage. Alcohol is mainly metabolized by the liver. Long-term and excessive drinking will significantly increase the liver burden, which may lead to fatty liver, alcoholic hepatitis, liver fibrosis, and even develop into liver cirrhosis and liver cancer. 2) Gastrointestinal problems. Components such as alcohol will stimulate the gastrointestinal mucosa. Long-term and excessive drinking is likely to cause digestive system diseases such as gastritis and gastric ulcers.

[0005] 3) The intake of beer and liquor is closely related to hyperuricemia. During the metabolism of alcohol in the body, it will interfere with the normal metabolism of uric acid by the liver, resulting in an increase in uric acid production. At the same time, alcohol may also affect the function of the kidneys and reduce the excretion of uric acid. In addition, beer itself also contains a certain amount of purine, which will be further metabolized into uric acid in the body, thus increasing the source of uric acid. Drinking alcohol leads to a continuous increase in uric acid in the body by increasing the source of uric acid and inhibiting the excretion of uric acid, thereby increasing the risk of developing hyperuricemia. 4) Increased risk of cardiovascular diseases. Excessive drinking will cause an increased burden on the heart, which may lead to problems such as arrhythmia and tachycardia. In addition, alcohol will also affect lipid metabolism and increase the risk of atherosclerosis, thereby leading to cardiovascular diseases such as hypertension and coronary heart disease. 5) Damage to the nervous system. Long-term and excessive drinking will manifest as symptoms such as memory loss, inattention, and slow reaction. In severe cases, it may also present with alcoholic encephalopathy, manifested as mental symptoms such as cerebellar ataxia, auditory and visual hallucinations.

[0006] Therefore, it is of great significance to develop alcoholic beverages with health care functions. Summary of the Invention

[0007] The object of the present invention is to provide a polygonatum sibiricum extract and its application in the preparation of functional alcoholic beverages, so as to solve the problems that excessive consumption of beer can lead to an increase in uric acid levels and a risk of hyperuricemia, and excessive consumption of white liquor can cause liver damage, and to provide a functional additive for beer and white liquor.

[0008] To achieve the above object, the present invention provides a method for preparing a polygonatum sibiricum extract, the steps are as follows:

[0009] S1. Wash the fresh polygonatum sibiricum rhizomes, dry them in a cool place and then slice them;

[0010] S2. Bake the slices obtained in S1 at 60-80°C for 5-10 minutes, and then continue to bake at 120°C until the polygonatum sibiricum rhizome slices turn golden yellow;

[0011] S3. Grind the golden yellow polygonatum sibiricum rhizome slices into powder and sieve them to obtain polygonatum sibiricum powder. Add distilled water to the polygonatum sibiricum powder and boil it. After standing, filter it, and collect the filtrate to obtain the aqueous solution of polygonatum sibiricum extract;

[0012] S4. Add absolute ethanol to the obtained aqueous solution of polygonatum sibiricum extract, mix well and then filter again to obtain the purified aqueous solution of polygonatum sibiricum extract;

[0013] S5. After rotary evaporation and concentration of the obtained purified aqueous solution of polygonatum sibiricum extract, freeze-dry it to obtain the polygonatum sibiricum extract.

[0014] Preferably, in the step S1, the fresh polygonatum sibiricum rhizomes are washed with drinking water; the slice thickness is 1-2 cm.

[0015] Preferably, in the step S3, the sieve aperture is not less than 100 mesh; the volume ratio of polygonatum sibiricum powder to distilled water is 1:5.

[0016] Preferably, in the step S3, the boiling is carried out at normal temperature and pressure for 5-10 minutes; the standing is carried out at normal temperature for 5-15 minutes; the filtration is carried out at normal temperature and pressure by a method that can effectively filter out visible residues.

[0017] Preferably, in the step S4, the addition amount of absolute ethanol is such that the alcohol content of the solution reaches the target degree.

[0018] Preferably, the target degree is the final alcohol content of the product to which the polygonatum sibiricum extract is to be applied.

[0019] A polygonatum sibiricum extract prepared by the preparation method as described above.

[0020] An application of the polygonatum sibiricum extract as described above in the preparation of functional beer, wherein the addition amount of the polygonatum sibiricum extract is 1 g of polygonatum sibiricum extract / L of beer; the functional beer is uric acid-lowering beer.

[0021] Use of the polygonatum extract as described above in the preparation of a functional liquor, wherein the addition amount of the polygonatum extract is 10 g of polygonatum extract / L of liquor; the functional liquor is a liquor for preventing acute alcoholic liver injury.

[0022] Therefore, the polygonatum extract provided by the present invention and its use in the preparation of a functional alcoholic beverage have the following specific technical effects:

[0023] (1) The polygonatum extract prepared by the present invention can be miscible with beer. Drinking beer added with the polygonatum extract prepared by the present invention can significantly reduce the increase in uric acid caused by drinking a large amount of beer, and can be used for the development of a beer with the function of reducing uric acid, which is of great significance for preventing hyperuricemia caused by beer;

[0024] (2) The polygonatum extract prepared by the present invention can be miscible with liquor. Drinking liquor added with the polygonatum extract prepared by the present invention can significantly reduce the increase in AST and ALT caused by drinking a large amount of liquor, and can be used for the development of a functional liquor for preventing acute alcoholic liver injury, which is of great significance for preventing acute alcoholic liver injury of consumers;

[0025] (3) The polygonatum extract provided by the present invention does not affect the appearance quality and taste of beer and liquor. The preparation method is simple and easy to operate, does not require special equipment, does not require high temperature and high pressure, the preparation process is safe, no toxic and harmful substances are generated, energy-saving and environmentally friendly, and is suitable for large-scale production. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for the description of the embodiments of the present invention will be briefly introduced below. Obviously, the drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.

[0027] Figure 1 is a photograph of the polygonatum extract prepared in Example 1 of the present invention;

[0028] Figure 2 is a photograph of the beer used in Application Example 1 of the present invention (A) and a photograph of the beer after dissolving the polygonatum extract prepared in Example 1 in the beer (B);

[0029] Figure 3 is a photograph of the polygonatum extract prepared in Example 2 of the present invention;

[0030] Figure 4 is a photograph of the alcohol used in Application Example 3 of the present invention (A) and a photograph of the liquor after dissolving the polygonatum extract prepared in Example 2 in the liquor (B). DETAILED DESCRIPTION OF THE INVENTION

[0031] The technical solution of the present invention will be further described below with reference to the accompanying drawings and embodiments.

[0032] In order to make the purpose, technical solution and advantages of the present application more clear, thorough and complete, the technical solution of the present invention will be clearly and completely described below through the accompanying drawings and embodiments. The following detailed descriptions are all descriptions of embodiments, aiming to provide further detailed descriptions of the present invention. Unless otherwise specified, all technical terms adopted by the present invention have the same meaning as commonly understood by those of ordinary skill in the art to which the present application belongs.

[0033] The instruments, equipment, reagents and materials used in the examples were all obtained through commercial channels.

[0034] Example 1

[0035] Prepare a polygonatum extract, and the specific steps are as follows:

[0036] S11. Wash the fresh polygonatum rhizome, dry it in a cool place, and then cut the dried polygonatum rhizome into slices with a thickness of 1 - 2 cm using a slicer.

[0037] S12. Set the oven to 70 °C, dry the polygonatum rhizome slices obtained in step S1 for 10 minutes, then adjust the oven temperature to 120 °C, and continue to bake at 120 °C for 2 minutes until the polygonatum rhizome slices turn golden yellow.

[0038] S13. Grind the golden yellow polygonatum rhizome slices into powder, pass through a 100 - mesh sieve, weigh 500 g of the sieved polygonatum powder, add 2.5 L of distilled water, boil for 5 minutes under normal temperature and pressure, then let it stand, filter to remove visible residues with four - layer gauze, collect the filtrate, and obtain an aqueous solution of polygonatum extract, with a total of 2.2 L.

[0039] S14. Add 120 mL of absolute ethanol to the obtained aqueous solution of polygonatum extract to make the alcohol content of the solution 5%, mix well, and then filter again with four - layer gauze to remove the precipitate, thus obtaining a purified aqueous solution of polygonatum extract.

[0040] S15. Use a rotary evaporator to perform rotary evaporation and concentration on the obtained purified aqueous solution of polygonatum extract to obtain a concentrated solution of polygonatum extract, and then use a vacuum freeze - dryer to perform freeze - drying on the concentrated solution of polygonatum extract, thus obtaining polygonatum extract, with a total of 65.07 g. The prepared polygonatum extract is as Figure 1 shown.

[0041] Application Example 1

[0042] Investigate the effect of the polygonatum extract prepared in Example 1 on the uric acid of mice that drank a large amount of beer. The specific steps are as follows:

[0043] Experimental animals: Male Kunming mice, weighing 18 - 22 g, 8 mice in each group.

[0044] Animal source: Jinan Pengyue Experimental Animal Breeding Co., Ltd.

[0045] Grouping and treatment:

[0046] ① Normal group: Kunming mice were intragastrically administered 0.5 mL of distilled water for 3 consecutive days, once a day. After the third intragastric administration, the blood samples were collected after 8 hours of fasting to detect the uric acid level.

[0047] ② Beer group: Kunming mice were intragastrically administered 0.5 mL of beer for 3 consecutive days, once a day. After the third intragastric administration, the blood samples were collected after 8 hours of fasting to detect the uric acid level.

[0048] ③ Polygonatum sibiricum beer group: Kunming mice were intragastrically administered 0.5 mL of beer (Polygonatum sibiricum extract was added at a ratio of 0.1%, that is, 0.1 g of the Polygonatum sibiricum extract prepared in Example 1 was added to 100 mL of beer) for 3 consecutive days, once a day. After the third intragastric administration, the blood samples were collected after 8 hours of fasting to detect the uric acid level. The photo of the beer (B) after the Polygonatum sibiricum extract was dissolved in it and the beer (A) without any treatment is as Figure 2 shown. There is no visible precipitate after the Polygonatum sibiricum extract is dissolved in the beer.

[0049] The uric acid detection results are shown in Table 1. The results show that after the mice drank beer, the uric acid level increased significantly, while the increase in the uric acid level of the mice that drank the beer added with Polygonatum sibiricum extract was extremely significantly lower than that of the mice that drank beer alone. It shows that the Polygonatum sibiricum extract prepared in Example 1 can be added to beer to effectively prevent the increase in uric acid caused by drinking beer.

[0050] Table 1

[0051] Group Uric acid (μmol / L) Normal group 245.37±32.11 Beer group <![CDATA[892.58±103.86 ## > Polygonatum sibiricum beer group <![CDATA[567.92±89.14 ** >

[0052] Note: ## Indicates P ≤ 0.01 compared with the normal group; ** Indicates P ≤ 0.01 compared with the beer group.

[0053] Application Example 2

[0054] Investigate the effect of the Polygonatum sibiricum extract prepared in Example 1 on the uric acid level of those who drink a large amount of beer. The specific steps are as follows:

[0055] Recruit 30 healthy volunteers aged 20 - 30, including 15 males and 15 females.

[0056] Grouping and treatment:

[0057] ① Normal group: Starting at 20:00, drink 1000 mL of distilled water within half an hour, do not drink water during this period, and collect blood samples after 12 h to detect uric acid levels.

[0058] ② Beer group: Starting at 20:00, drink 1000 mL of beer within half an hour, do not drink water during this period, and collect blood samples after 12 h to detect uric acid levels.

[0059] ③ Polygonatum sibiricum beer group: Starting at 20:00, drink 1000 mL of beer (add the Polygonatum sibiricum extract prepared in Example 1 at a ratio of 0.1%, that is, add 0.1 g of the Polygonatum sibiricum extract prepared in Example 1 to 100 mL of beer) within half an hour, and collect blood samples after 12 h to detect uric acid levels.

[0060] The test results are shown in Table 2. The results show that after the volunteers drank beer, the uric acid levels increased significantly, while the increase in uric acid levels of the volunteers who drank the beer added with the Polygonatum sibiricum extract prepared in Example 1 was extremely significantly lower than that of the volunteers who drank beer alone. It shows that the Polygonatum sibiricum extract prepared in Example 1 can be added to beer to effectively prevent the increase in uric acid caused by drinking beer.

[0061] Table 2

[0062] Group Uric acid (μmol / L) Normal group 283.37±40.23 Beer group <![CDATA[695.38±126.09 ## > Polygonatum sibiricum beer group <![CDATA[427.94±97.22 ** >

[0063] Note: ## Indicates P ≤ 0.01 compared with the normal group; ** Indicates P ≤ 0.01 compared with the beer group.

[0064] Example 2

[0065] Prepare a Polygonatum sibiricum extract, and the specific steps are as follows:

[0066] S21. Wash the fresh Polygonatum sibiricum rhizomes, dry them in a cool place, and then use a slicing machine to cut the dried Polygonatum sibiricum rhizomes into slices with a thickness of 1 - 2 cm.

[0067] S22. Set the oven to 80 °C, dry the Polygonatum sibiricum rhizome slices obtained in step S21, bake for 5 minutes, then adjust the oven temperature to 120 °C, and continue to bake at 120 °C for 2 minutes until the Polygonatum sibiricum rhizome slices turn golden yellow.

[0068] S23. Grind the golden Polygonatum sibiricum rhizome slices into powder, pass through a 100 - mesh sieve, weigh 500 g of the sieved Polygonatum sibiricum powder, add 2.5 L of distilled water, boil for 5 minutes at normal temperature and pressure, then let it stand, filter with four - layer gauze to remove visible residues, and collect the filtrate to obtain an aqueous solution of Polygonatum sibiricum extract, with a total of 2.2 L.

[0069] S24. Add 120 mL of absolute ethanol to the obtained aqueous solution of polygonatum sibiricum extract to make the alcohol content of the solution 50%. After mixing evenly, filter again through four layers of gauze to remove the precipitate, and then the purified aqueous solution of polygonatum sibiricum extract is obtained.

[0070] S25. Use a rotary evaporator to perform rotary evaporation and concentration on the obtained purified aqueous solution of polygonatum sibiricum extract to obtain a concentrated solution of polygonatum sibiricum extract. Then, use a vacuum freeze dryer to perform freeze drying on the concentrated solution of polygonatum sibiricum extract, and then the polygonatum sibiricum extract is obtained, with a total of 60.07 g. The photo of the prepared polygonatum sibiricum extract is as Figure 4 shown.

[0071] Application Example 3

[0072] Investigate the effect of the polygonatum sibiricum extract prepared in Example 2 on liver injury in mice with excessive consumption of white liquor. The specific steps are as follows:

[0073] Experimental animals: SD rats, weighing 200 ± 20 g, with 8 rats in each group.

[0074] Animal source: Jinan Pengyue Experimental Animal Breeding Co., Ltd.

[0075] Experimental grouping and treatment:

[0076] ① Normal group: Administer by gavage with distilled water, 7 mL / kg each time, twice a day, once in the morning and once in the evening, for 1 week continuously.

[0077] ② Acute alcoholic liver injury model group: Administer by gavage with 50% alcohol, 7 mL / kg each time, twice a day, once in the morning and once in the evening, for 1 week continuously.

[0078] ③ Polygonatum sibiricum group: Administer by gavage with 50% alcohol (adding the polygonatum sibiricum extract prepared in Example 2 according to a 1% ratio, that is, adding 1 g of the polygonatum sibiricum extract prepared in Example 2 to 100 mL), 7 mL / kg each time, twice a day, once in the morning and once in the evening, for 1 week continuously. The photo of the alcohol added with the polygonatum sibiricum extract is as Figure 4 shown, and there is no visible precipitate.

[0079] During the experiment, the rats in each group drank water freely and were fed with ordinary feed conventionally. After the experiment ended, the contents of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in the serum of the rats in each group were detected. The results are shown in Table 3. Compared with the normal group, the contents of AST and ALT in the serum of the rats in the acute alcoholic liver injury model group were significantly increased. Compared with the acute alcoholic liver injury model group, the contents of AST and ALT in the serum of the rats drinking alcohol containing the polygonatum sibiricum extract prepared in Example 2 were extremely significantly decreased. It shows that the polygonatum sibiricum extract prepared in Example 2 can effectively prevent acute alcoholic liver injury.

[0080] Table 3

[0081] Group AST (U / L) ALT (U / L) Normal group 73.67±5.98 31.90±3.07 Acute alcoholic liver injury model group <![CDATA[314.84±28.97 ## > <![CDATA[154.49±14.87 ## > Polygonatum sibiricum group <![CDATA[149.22±12.99 ** > <![CDATA[85.31±7.34 ** >

[0082] Note: ## Indicates P≤0.01 when compared with the normal group; ** Indicates P≤0.01 when compared with the acute alcoholic liver injury model group. Therefore, the polygonatum extract prepared by the present invention can be miscible with beer and white liquor. Drinking beer added with the polygonatum extract prepared by the present invention can significantly reduce the increase in uric acid caused by drinking a large amount of beer; drinking white liquor added with the polygonatum extract prepared by the present invention can significantly reduce the increase in AST and ALT caused by drinking a large amount of white liquor, and can be used for the development of functional beer for reducing uric acid and functional white liquor for preventing acute alcoholic liver injury; the provided polygonatum extract does not affect the appearance quality and taste of beer and white liquor, the preparation method is simple and easy to operate, does not require special equipment, does not require high temperature and high pressure, the preparation process is safe, no toxic and harmful substances are generated, energy-saving and environmental-friendly, and is suitable for large-scale production.

[0083] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit them. Although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that they can still modify or equivalently replace the technical solutions of the present invention, and these modifications or equivalent replacements cannot make the modified technical solutions deviate from the spirit and scope of the technical solutions of the present invention.

Claims

1. A method for preparing a polygonatum extract, characterized in that: Here are the steps: S1. Clean the fresh rhizomes of Polygonatum cyrtonema, dry them in a cool place and slice them; S2, baking the slices obtained in S1 at 60-80° C. for 5-10 minutes, and then continuing to bake at 120° C. until the slices of the rhizome of Polygonatum cyrtonema turn golden yellow; S3, grinding and sieving the golden rhizome of Polygonatum cyrtonema to obtain Polygonatum cyrtonema powder, adding distilled water to the Polygonatum cyrtonema powder and boiling, filtering after standing, collecting the filtrate, and obtaining the Polygonatum cyrtonema extract aqueous solution; S4, adding anhydrous ethanol to the obtained aqueous solution of polygonatum sibiricum extract, mixing and filtering again to obtain a purified aqueous solution of polygonatum sibiricum extract; S5. Concentrating the purified aqueous solution of polygonatum sibiricum extract by rotary evaporation and freeze-drying to obtain the polygonatum sibiricum extract.

2. The method for preparing a polygonatum sibiricum extract according to claim 1, characterized in that: In step S1, the fresh rhizome of Polygonatum cyrtonema is washed with drinking water; and the thickness of the slices is 1 to 2 cm.

3. The method for preparing a polygonatum extract according to claim 1, characterized in that: The sieved particle size in step S3 is not less than 100 mesh; the volume ratio of polygonatum sibiricum powder to distilled water is 1:

5.

4. The method for preparing a polygonatum sibiricum extract according to claim 1, characterized in that: In the step S3, the boiling is performed at room temperature and pressure for 5 to 10 minutes; the standing is performed at room temperature and pressure for 5 to 15 minutes; and the filtering is performed at room temperature and pressure using a method that can effectively filter out visible residues.

5. The method for preparing a polygonatum sibiricum extract according to claim 1, characterized in that: The amount of anhydrous ethanol added in step S4 is such that the alcohol content of the solution reaches the target degree.

6. The method for preparing a polygonatum sibiricum extract according to claim 5, characterized in that: The target alcohol content is the final alcohol content of the product to which the Polygonatum sibiricum extract is to be applied.

7. A polygonatum extract prepared by the preparation method according to any one of claims 1 to 6.

8. A use of the polygonatum sibiricum extract as claimed in claim 7 in preparing functional beer, characterized in that: The addition amount of the polygonatum sibiricum extract is 1g polygonatum sibiricum extract / L beer; and the functional beer is uric acid-lowering beer.

9. A use of the polygonatum sibiricum extract as claimed in claim 7 in the preparation of functional liquor, characterized in that: The addition amount of the polygonatum sibiricum extract is 10g polygonatum sibiricum extract / L liquor; the functional liquor is liquor for preventing acute alcoholic liver damage.

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