Multifunctional magnetic bead purification instrument

Abstract

The invention discloses a multifunctional magnetic bead purification instrument which comprises a base, a magnetic extraction mechanism, a pipetting module and a control system, wherein the magnetic extraction mechanism and the pipetting module are mounted on the base; the control system is used for controlling the magnetic extraction mechanism and the pipetting module; the magnetic extraction assembly comprises a magnetic bar assembly, a magnetic bar frame used for fixing the magnetic bar assembly and a first power system, and the first power system enables the magnetic bar assembly to reciprocate on the base in the X-axis direction, the Y-axis direction and the Z-axis direction. The pipetting module comprises a pipetting assembly and a second power system, and the second power system enables the pipetting assembly to do reciprocating motion on the base in the X-axis direction, the Y-axis direction and the Z-axis direction; a placing disc is arranged on the base, and a plurality of perforated plates are arranged on the placing disc. The structure of an existing magnetic bead purification instrument is improved, magnetic bead purification and extracting solution transfer are integrated, and the automatic process from extraction to transfer is achieved. The multifunctional magnetic bead purifier can be used for purifying and extracting nucleic acid, protein and plasmids.

Description

Technical Field

[0001] The present application relates to a magnetic bead purification device, and more particularly to a multifunctional magnetic bead purifier. Background Art

[0002] A magnetic bead purifier adsorbs target molecules (such as proteins, DNA, or RNA, etc.) on the surface of magnetic beads through the magnetism of magnetic microparticles, and then uses magnetic force to separate the magnetic beads and the adsorbed molecules, thereby realizing the separation and purification of biological samples. Magnetic bead purifiers are mainly used for the rapid purification of nucleic acids, proteins, and cells. By adopting magnetic bead separation and extraction purification technology, they can automatically perform steps such as mixing, magnetic bead transfer, washing, and elution. Magnetic bead purifiers have wide application value in the fields of biomedical research, clinical diagnosis, drug development, etc. It can quickly and efficiently separate and purify target molecules in biological samples, providing high-quality samples for subsequent experiments and analyses, and playing an important role in the fields of biomedical research and clinical diagnosis. Magnetic bead nucleic acid purifiers can be widely used in the fields of genomics, disease control and medical treatment, food safety, forensic identification, etc.

[0003] When a magnetic bead purifier purifies substances such as nucleic acids, proteins, and cells, the main process flow includes steps such as magnetic bead pretreatment, binding of magnetic beads to target molecules, magnetic bead washing, and separation and purification of magnetic beads from samples. Currently, the structures of commonly used magnetic bead purifiers mainly include an auxiliary agent system, a magnetic force system, a liquid handling system, etc. Existing magnetic bead purifiers can efficiently and rapidly separate and purify substances such as nucleic acids, proteins, and cells, but there are still some deficiencies and defects; for example, the problem of magnetic beads remaining in samples or purified products; although some magnetic bead purifiers have achieved high automation, there are still some instruments that require manual operation or have relatively complex operation processes and cannot meet the experimental requirements of large-scale or high-throughput experiments. Summary of the Invention

[0004] In order to overcome the deficiencies of the prior art, the purpose of the present application is to provide a multifunctional magnetic bead purifier, which integrates magnetic bead purification and extract transfer by improving the structure of the existing magnetic bead purifier, and realizes the automated process from extraction to transfer.

[0005] To solve the above problems, the technical solutions adopted in the present application are as follows:

[0006] An embodiment of the present application provides a multifunctional magnetic bead purifier, including a base, a magnetic extraction mechanism and a pipetting module installed on the base, and a control system for controlling the magnetic extraction mechanism and the pipetting module;

[0007] The magnetic extraction component includes a magnetic rod component, a magnetic rod holder for fixing the magnetic rod component, and a first power system, and the first power system enables the magnetic rod component to reciprocate along the X-axis, Y-axis, and Z-axis directions on the base respectively;

[0008] The pipetting module includes a pipetting component and a second power system, and the second power system enables the pipetting component to reciprocate along the X-axis, Y-axis, and Z-axis directions on the base respectively;

[0009] A placement tray is arranged on the base, and a plurality of porous plates are arranged on the placement tray.

[0010] As a further preferred solution, the magnetic rod assembly described in the embodiment of the present application includes a plurality of magnetic rods and magnetic rod sleeves corresponding to the number of magnetic rods. The magnetic rod holder includes a magnetic rod fixing plate and a magnetic rod sleeve fixing plate. The upper ends of the plurality of magnetic rods are fixed on the magnetic rod fixing plate; the magnetic rod sleeve fixing plate is located directly below the magnetic rod fixing plate. Through holes equal in number to the magnetic rods are arranged on the magnetic rod sleeve fixing plate corresponding to the positions of the magnetic rods. The open ends of the magnetic rod sleeves are fixed at the through holes, and the magnetic rods can be driven by the first power system to pass through the through holes and be sleeved into the magnetic rod sleeves.

[0011] As a further preferred solution, the first power system described in the embodiment of the present application includes a first horizontal moving device and a first lifting device; the first horizontal moving device includes a first X-axis moving component for moving the magnetic rod assembly along the X-axis direction on the base and a Z-axis moving component for moving the magnetic rod assembly along the Z-axis direction on the base; the first lifting component includes a first lifting motor and a lifting structural member for providing a lifting path for the magnetic rod assembly.

[0012] As a further preferred solution, the first X-axis moving component described in the embodiment of the present application includes a first driving motor, an X-axis guide rail, and a first sliding seat installed on the X-axis guide rail. The first sliding seat is connected to the first driving motor, and the magnetic rod holder is installed on the first sliding seat; the lifting structural member is installed on the first sliding seat and connected to the magnetic rod holder; the Z-axis moving component includes a second driving motor, a first ball screw, a first ball nut, and a fixing frame for fixing the second driving motor. The bottom of the X-axis guide rail is fixedly connected with a mounting plate. The first ball screw is fixed on one side of the mounting plate, and the first ball nut is fitted and installed on the first ball screw. A limiting structure for preventing the first ball nut from rotating with the first ball nut is arranged at the lower end of the mounting plate. The magnetic extraction mechanism moves back and forth in the Z-axis direction as a whole under the drive of the second driving motor through the rotation of the first ball screw.

[0013] As a further preferred solution, the lifting structural member described in the embodiment of the present application includes a second ball screw and a second ball nut. A receiving groove for installing the second ball screw is provided on the first sliding seat. The upper and lower ends of the second ball screw are fixed in the receiving groove. The second ball nut is installed on the second ball screw and fixedly connected to the magnetic rod holder. A card slot for restricting the rotation of the second ball nut is provided in the receiving groove along the Z-axis direction, and one side of the second ball nut is clamped in the card slot.

[0014] As a further preferred solution, the second power system described in the embodiment of the present application includes a moving seat, a second horizontal moving device, and a second lifting device; the moving seat is installed on the X-axis guide rail, the liquid transfer assembly is installed on the moving seat, the second lifting device drives the liquid transfer assembly to reciprocate along the Y-axis direction of the base relative to the moving seat, the second horizontal moving device drives the liquid transfer assembly to reciprocate along the X-axis direction of the base through the moving seat, and the Z-axis moving assembly drives the liquid transfer assembly to reciprocate along the z-axis direction of the base.

[0015] As a further preferred solution, the liquid transfer assembly described in the embodiment of the present application includes a liquid transfer arm, a peristaltic pump, a motor for controlling the stroke of the peristaltic pump, and an induction sensor for identifying the liquid level height in the pipette tip; the liquid transfer arm is installed on the moving seat and is driven by the second lifting device to reciprocate up and down relative to the moving seat. The peristaltic pump, the motor, and the induction sensor are installed on the liquid transfer arm, and a connector for connecting the pipette tip and the peristaltic pump is provided at the lower end of the liquid transfer arm.

[0016] As a further preferred solution, the second lifting device described in the embodiment of the present application includes a second lifting motor, a third ball screw, and a third ball nut fitted and installed on the ball screw. A chute is provided on the moving seat from top to bottom. One side of the third ball nut is installed in the chute, and the liquid transfer arm is fixed to the third ball nut. The second lifting motor is connected to the third ball screw through a coupling; the connector is provided with a plurality of annular steps arranged from top to bottom, and the diameters of the plurality of annular steps decrease from top to bottom for installing pipette tips of different calibers.

[0017] As a further preferred solution, a bracket for placing pipette tips and a pipette tip ejection assembly are further provided on the base of the present application embodiment. The pipette tip ejection assembly includes a pipette tip collection groove and a pipette tip ejection rack, and an opening groove matching the open end of the pipette tip is provided on the pipette tip ejection rack.

[0018] As a further preferred solution, the multifunctional magnetic bead purifier described in the embodiment of the present application further includes a liquid addition module. The liquid addition module includes a plurality of liquid injection tubes, a liquid injection pump connected to the liquid injection tubes, a motor for providing power to the liquid injection pump, and a third lifting device. The liquid injection tubes and the liquid injection pump are fixedly installed on the moving seat facing away from the liquid transfer assembly.

[0019] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0020] 1. The magnetic rod assembly of the multifunctional magnetic bead purifier described in the present application achieves precise displacement (±0.01 mm) in the X / Y / Z axes through the first power system, can cover any well position of the multi-well plate, and complete the operations of magnetic bead adsorption, transfer, and release. Compared with traditional single-axis or manual devices, the processing efficiency is increased by more than 50%; the magnetic rod assembly adopts a permanent magnet design, adsorbs magnetic beads by lifting along the Z axis into the well and then transfers them to the next station, avoiding direct contact with liquid throughout the process and reducing the risk of cross-contamination.

[0021] 2. The pipetting module of the multifunctional magnetic bead purifier described in the present application independently controls the pipette to move in the X / Y / Z axes through the second power system, and can synchronously perform steps such as liquid addition (diluent, washing solution, etc.) and waste liquid suction; it shortens the process time. For example, when the magnetic rod transfers magnetic beads to the washing well, the pipetting arm simultaneously injects the buffer solution to achieve seamless connection.

[0022] 3. Further, the multifunctional magnetic bead purifier described in the present application greatly improves the flexibility and application range of the instrument, enabling the magnetic rod assembly to accurately locate to any position in the multi-well plate for the extraction of magnetic particles, and at the same time, the pipetting assembly can also accurately aspirate and release the sample liquid.

[0023] 4. Further, in the multifunctional magnetic bead purifier described in the present application, since both the magnetic rod assembly and the pipetting module can move freely in three dimensions, this instrument can adapt to sample containers of different sizes and shapes, as well as different types of magnetic beads and reagents; the multifunctional magnetic bead purifier described in the present application can process multiple samples simultaneously, improving the processing throughput; at the same time, due to the high flexibility of the magnetic rod assembly and the pipetting module, the instrument can also adapt to multi-well plates of different specifications and types, further enhancing its versatility.

[0024] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments. Description of the Drawings

[0025] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the accompanying drawings required for the description of the embodiments will be briefly introduced below. Obviously, the accompanying drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.

[0026] Figure 1 It is a schematic structural diagram of the multifunctional magnetic bead purifier described in the embodiments of the present application.

[0027] Figure 2Structural schematic diagram of the magnetic extraction mechanism described in the embodiments of the present application.

[0028] Figure 3 Structural schematic diagram of the liquid transfer module and the liquid addition module described in the embodiments of the present application.

[0029] Figure 4 Structural diagram of the connector described in the embodiments of the present application.

[0030] Among them, each reference numeral is: 10, base; 20, magnetic extraction mechanism; 30, liquid transfer module; 21, magnetic bar assembly; 211, magnetic bar; 212, magnetic bar sleeve; 22, magnetic bar holder; 221, magnetic bar fixing plate; 222, magnetic bar sleeve fixing plate; 23, first power system; 231, first horizontal moving device; 232, first lifting device; 31, liquid transfer assembly; 311, liquid transfer arm; 312, peristaltic pump; 313, connector; 32, second power system; 321, moving seat; 322, second horizontal moving device; 323, second lifting device; 40, bracket for liquid transfer gun head; 50, gun head retraction assembly; 51, collection tank; 52, gun head retraction frame; 60, liquid addition module; 61, injection tube; 62, injection pump; 63, motor. Detailed implementation manners

[0031] To make the objectives, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present application. Obviously, the described embodiments are some, but not all, of the embodiments of the present application. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present application without creative efforts shall fall within the protection scope of the present application.

[0032] The term "including" and other descriptive manners equivalent thereto involved in the description and claims of the present application are all intended to cover non-exclusive inclusion, that is, it includes both the content clearly described in the description and claims, and also includes steps or units inherent in the product, method or structure that are not described in the description and claims.

[0033] Such as Figure 1As shown in the figure, an embodiment of the present application provides a multifunctional magnetic bead purifier, which includes a base 10, a magnetic extraction mechanism 20 and a pipetting module 30 installed on the base 10, and a control system (not shown in the figure) for controlling the magnetic extraction mechanism 20 and the pipetting module 30; the magnetic extraction component includes a magnetic rod assembly 21, a magnetic rod holder 22 for fixing the magnetic rod assembly 21, and a first power system 23, and the first power system enables the magnetic rod assembly 21 to reciprocate along the X-axis, Y-axis, and Z-axis directions on the base 10 respectively; the magnetic extraction component 20 is one of the core components, which can accurately locate to any position in the multi-well plate, adsorb the magnetic beads combined with the target molecules, and separate the magnetic beads from the sample by moving. The magnetic rod assembly 21 adsorbs and releases the magnetic beads, thereby realizing the purification of the sample. The pipetting module 30 includes a pipetting component 31 and a second power system 32, and the second power system 32 enables the pipetting component 31 to reciprocate along the X-axis, Y-axis, and Z-axis directions on the base respectively; the pipetting module 30 is responsible for the aspiration, transfer, and release of the sample liquid, and transfers the sample liquid from the multi-well plate through precise movement and positioning. The three-dimensional movement ability of the magnetic extraction mechanism 20 and the pipetting module 30 greatly improves the flexibility and application range of the instrument, and realizes the automation of the entire purification process. A placement tray is provided on the base, and a plurality of multi-well plates are provided on the placement tray; the placement tray can be adapted to standard consumables such as 96-well plates, deep-well plates, and PCR plates, and can be quickly replaced through a snap-fit structure to meet different experimental scenarios. The main control module of the control system adopted in the embodiment of the present application can select a multi-core PLC to be responsible for coordinating the timing logic of the magnetic bead extraction and pipetting processes, and realizing multi-axis synchronous control. The displacement of the magnetic rod, the pipetting volume, and the position of the magnetic rod sleeve can be detected by setting sensor interfaces and the detection signals are transmitted to the main control module, and the main control module judges whether to give instructions to the magnetic extraction mechanism and the pipetting module according to the received signals.

[0034] As Figure 2As shown, in the embodiments of the present application, the magnetic extraction component 20 can generate a magnetic field with a specific intensity and distribution. The magnetic rod component 21 includes a plurality of magnetic rods 211 and magnetic rod sleeves 212 corresponding to the number of magnetic rods 211. Each magnetic rod 211 is equipped with an independent magnetic rod sleeve 212 to form a physical isolation barrier to avoid cross-contamination caused by magnetic beads or liquid residues. The number of magnetic rods 211 can be set according to the number of pores in the multi-well plate. For example, when using a 24-well plate, the number of magnetic rods 211 can be set to 24. The magnetic rod sleeves 212 can be used once or quickly disassembled and cleaned to adapt to high-throughput scenarios. The magnetic rod holder 22 includes a magnetic rod fixing plate 221 and a magnetic rod sleeve fixing plate 222. The upper ends of a plurality of the magnetic rods 211 are fixed on the magnetic rod fixing plate 221; the magnetic rod sleeve fixing plate 222 is located directly below the magnetic rod fixing plate 221. Through holes equal in number to the magnetic rods are provided on the magnetic rod sleeve fixing plate 222 corresponding to the positions of the magnetic rods. The open ends of the magnetic rod sleeves are fixed at the through holes, and the through holes correspond to the magnetic rods 211 one by one to ensure the precise installation position of the magnetic rod sleeves 212; the magnetic rod sleeves 212 are precisely positioned through the magnetic rod sleeve fixing plate 222 to ensure that there is no contact between the magnetic rod 211 and the inner wall of the sleeve when inserted, reducing wear; the magnetic rod 211 can be driven by the first power system to pass through the through hole and be sleeved into the magnetic rod sleeve 212; during the magnetic bead adsorption stage, the magnetic rod moves down through the through hole and is completely sleeved into the magnetic rod sleeve, and the magnetic force passes through the sleeve to adsorb the magnetic beads, avoiding liquid contact with the magnetic rod body; during the transfer and release stage, after the magnetic rod carrying the magnetic bead sleeve is transferred to the target hole position, the magnetic beads are made to fall off by adsorbing the magnetic beads with a permanent magnet outside the multi-well plate, and the magnetic rod sleeve remains fixed in the original plate position, reducing the complexity of moving parts.

[0035] In some specific embodiments of the present application, the first power system 23 includes a first horizontal moving device 231 and a first lifting device 232; the first horizontal moving device 231 includes a first X-axis moving component for moving the magnetic rod assembly along the X-axis direction on the base and a Z-axis moving component for moving the magnetic rod assembly along the Z-axis direction on the base; the first X-axis moving component includes a first driving motor, an X-axis guide rail, and a first sliding seat mounted on the X-axis guide rail. The first driving motor can be a servo motor or a stepper motor; since the servo motor performs better in terms of accuracy, speed, and torque control and can achieve more precise positioning and fast response, a servo motor is preferably used; the X-axis guide rail provides a guiding function for the moving part to ensure that it moves linearly along the X-axis direction. The X-axis guide rail adopts a linear guide rail, which can effectively reduce the frictional resistance and improve the smoothness and accuracy of the movement; the first sliding seat is mounted on the X-axis guide rail and is used in conjunction with the axial guide rail to freely slide on the X-axis guide rail under the power provided by the first driving motor. The first sliding seat is connected to the first driving motor, the magnetic rod holder is mounted on the first sliding seat, the lifting structure member is mounted on the first sliding seat and is connected to the magnetic rod holder, and the magnetic rod is fixed on the magnetic rod holder. Driven by a stepper motor or a servo motor, the first sliding seat moves along the X-axis guide rail, realizing the movement of the magnetic rod in the X-axis direction. In the embodiments of the present application, the Z-axis moving component includes a second driving motor, a first ball screw, a first ball nut, and a fixing bracket for fixing the second driving motor. The bottom of the X-axis guide rail is fixedly connected with a mounting plate. The first ball screw is fixed on one side of the mounting plate, the first ball nut is fitted and mounted on the first ball screw, and a limiting structure for preventing the first ball nut from rotating with the first ball screw is provided at the lower end of the mounting plate. The limiting structure can be a slot provided in the mounting plate to cooperate with one side of the first ball nut, the purpose of which is to enable one side of the first ball nut to be clamped with it. When the first ball screw rotates, the first ball nut does not rotate, converting the rotational motion into a linear motion to meet the reciprocating motion of the magnetic extraction mechanism in the Z-axis direction under the drive of the second driving motor through the rotation of the first ball screw. In some other embodiments, the first ball screw and the first ball nut can be replaced with a combination of a linear guide rail and a slider. For example, a Z-axis direction guide rail is provided on the basis of the above-mentioned scheme, and the mounting plate is replaced with a slider. Such a scheme can also realize the reciprocating motion of the magnetic extraction mechanism in the Z-axis direction. Of course, the combination of the ball nut and the ball screw is easier to control in terms of accuracy than the combination of the guide rail and the slider, but the combination of the guide rail and the slider is simpler in terms of the combined design. In addition, the combination of a rack and a gear can be used to replace the combination of the ball nut and the ball screw. In the present application, the second driving motor can also be a stepper motor or a servo motor.

[0036] In the embodiment of the present application, the first lifting assembly 232 provides a lifting path for the magnetic rod assembly, enabling the magnetic rod to perform precise lifting movements in the vertical direction to complete operations such as adsorption, transfer, and release of magnetic beads. On the basis of the above solution, the first lifting assembly 232 adopted includes a first lifting motor and a lifting structural member that provides a lifting path for the magnetic rod assembly. Specifically, the lifting structural member includes a second ball screw and a ball nut. A receiving groove for installing the second ball screw is provided on the first sliding seat, and the receiving groove is opened from top to bottom. In addition to facilitating the installation of the second ball screw, the receiving groove can also save space to achieve the miniaturized design of the magnetic bead purifier. The upper and lower ends of the second ball screw are fixed in the receiving groove, the second ball nut is installed on the second ball screw and fixedly connected to the magnetic rod holder, and a card slot for restricting the rotation of the second ball nut is provided in the receiving groove along the Z-axis direction. One side of the second ball nut is clamped in the card slot. The precise control of the first lifting device can ensure that the lifting actions of the magnetic rod are accurate in different stages. For example, in the magnetic bead adsorption stage, the magnetic rod can accurately move down to a suitable position to adsorb magnetic beads, and in the transfer stage, the magnetic beads can be accurately transferred to the target hole position. As an alternative solution, a cylinder or a hydraulic cylinder can also be used to achieve the lifting of the magnetic rod assembly.

[0037] As a further preferred solution, the second power system 32 described in the embodiments of the present application includes a moving seat 321, a second horizontal moving device 322, and a second lifting device 323; the moving seat 321 is installed on the X-axis guide rail, the pipetting assembly is installed on the moving seat, and the second lifting device drives the pipetting assembly to reciprocate along the Y-axis direction of the base relative to the moving seat; the second horizontal moving device drives the pipetting assembly to reciprocate along the X-axis direction of the base through the moving seat, and the Z-axis moving assembly drives the pipetting assembly to reciprocate along the Z-axis direction of the base. The pipetting assembly 31 includes a pipetting arm 311, a peristaltic pump 312, a motor (not shown in the figure) for controlling the stroke of the peristaltic pump, and an induction sensor (not shown in the figure) for identifying the liquid level height in the pipette tip; the pipetting arm is installed on the moving seat and is driven by the second lifting device to reciprocate up and down relative to the moving seat, the peristaltic pump, the motor, and the induction sensor are installed on the pipetting arm, and a connector 313 for connecting the pipette tip and the peristaltic pump 312 is provided at the lower end of the pipetting arm 311. In this solution, the pipetting arm is installed on the moving seat. When the second lifting device is started, it drives the pipetting arm to move up and down along the Z-axis direction of the base according to a preset program, and accurately positions the connector at the lower end of the pipetting arm to the target liquid-taking position, such as above the hole position filled with the sample liquid in the multi-well plate; at the same time, the position of the pipetting assembly in the horizontal direction is determined by the second horizontal moving device driving the pipetting arm to move along the X-axis direction of the base through the moving seat, ensuring that the connector can accurately correspond to the target hole position; then the motor that controls the stroke of the peristaltic pump controlled by the control system starts to work. The motor accurately controls the stroke of the peristaltic pump according to the set pipetting volume in the experiment. The peristaltic pump generates suction by squeezing the pump tube with rollers and transmits it to the pipette tip through the connector. Under the action of the suction, the pipette tip sucks the sample liquid from the target hole position into the pipette tip; during the liquid-taking process, the induction sensor installed on the pipetting arm for identifying the liquid level height in the pipette tip monitors the rising situation of the liquid level in the pipette tip in real time. When the liquid level reaches the preset height, that is, when the required pipetting volume for the experiment is met, the induction sensor quickly transmits a signal to the control system, and the control system immediately controls the motor to stop the operation of the peristaltic pump, completing the liquid-taking operation and ensuring that the volume of the sucked sample liquid is accurate. After the liquid-taking is completed, the second lifting device works again, driving the pipetting arm to rise, so that the pipette tip filled with the sample liquid leaves the original liquid-taking hole position; then, the second horizontal moving device and the Z-axis moving assembly work together to drive the pipetting arm to move in the horizontal and vertical directions, accurately positioning the pipette tip to the target transfer position; when the pipette tip reaches the target transfer position, the motor rotates in the reverse direction, controlling the peristaltic pump to generate pressure, and discharging the sample liquid in the pipette tip through the connector and squeezing it out through the pipette tip, releasing it to the target position.

[0038] Specifically, in some embodiments, the second lifting device includes a second lifting motor, a third ball screw, and a third ball nut fitted on the ball screw. A chute extending from top to bottom is provided on the moving seat. One side of the third ball nut is installed in the chute, and the pipetting arm is fixed to the third ball nut. The second lifting motor is connected to the third ball screw through a coupling. The second horizontal movement device includes a third driving motor (which drives the pipetting assembly to complete the movement in the X-axis direction). After the second lifting motor is started, the rotational power is transmitted to the third ball screw through the coupling. Since the third ball nut is fitted on the ball screw and one side of it is installed in the chute extending from top to bottom on the moving seat, the chute restricts the rotation of the third ball nut, causing the third ball nut to only move up and down linearly along the chute under the drive of the screw. And the pipetting arm is fixed to the third ball nut. Therefore, as the third ball nut moves up and down, the pipetting arm will correspondingly move up and down reciprocally along the Z-axis direction of the base relative to the moving seat. This process realizes the precise position control of the pipetting arm in the vertical direction to meet the requirements of different height positions in the pipetting operation.

[0039] Further, in the embodiments of the present application, the connector 313 is provided with a plurality of annular steps arranged from top to bottom, and the diameters of the plurality of annular steps decrease from top to bottom for installing pipette tips of different calibers. When it is necessary to install pipette tips of different calibers, select an annular step with a suitable diameter according to the inner diameter size of the pipette tip. The diameter of each annular step is adapted to the inner diameter sizes of common pipette tips of different calibers. For example, the outer diameter of the topmost step can match a 5-mm caliber pipette tip, and the bottommost step can match a 1-mm caliber pipette tip, which can ensure a tight and stable connection between the pipette tip and the connector, preventing the pipette tip from falling off or leaking during the pipetting process. The design of multiple annular steps of the connector enables the same connector to be adapted to pipette tips of multiple calibers, eliminating the need to frequently replace the entire connector or the pipetting assembly, reducing the experimental preparation time, and improving the experimental efficiency.

[0040] As a further preferred solution, a bracket 40 for placing pipette tips and a tip ejection assembly 50 are further provided on the base 10 described in the embodiment of the present application. The tip ejection assembly 50 includes a tip collection groove 51 and a tip ejection rack 52. An opening groove matching the open end of the pipette tip is provided on the tip ejection rack. Preferably, an elastic material such as resin or rubber is used at the opening groove. In this solution, the pipette tip bracket is provided on the base and is specifically used for placing pipette tips. The shape and size of the bracket are customized according to the specifications of common pipette tips, and may adopt forms such as grooves, sockets or other suitable forms, so that the tips can be accurately placed on the bracket. The tip collection groove, as part of the tip ejection assembly, is used to collect used pipette tips. After the experimenter completes the pipetting operation, the used tips need to be ejected and discarded. An opening groove matching the open end of the pipette tip is provided on the tip ejection rack. When ejecting the tip, align the tip of the pipette with the opening groove on the tip ejection rack, and then push the pipette along the direction of the opening groove. Since an elastic material (such as plastic or rubber) is used at the opening groove, when the tip is inserted into the opening groove, the elastic material will exert a certain squeezing force on the tip, and this squeezing force can help the experimenter more easily separate the tip from the pipette.

[0041] As a further preferred solution, the multi-functional magnetic bead purifier described in the embodiment of the present application further includes a liquid addition module 60. The liquid addition module 60 includes a plurality of liquid injection tubes 61, a liquid injection pump 62 connected to the liquid injection tubes, a motor 63 for providing power to the liquid injection pump 62, and a third lifting device (not shown in the figure). The liquid injection tubes and the liquid injection pump are fixedly installed on the moving seat facing away from the pipetting assembly. Due to the liquid injection tubes, the movement of the liquid addition module in the X-axis and Z-axis directions can be powered by the second horizontal moving device and the Z-axis moving assembly respectively. The third lifting device can adopt the same structural design as the second lifting device. The liquid injection pump generates a pressure difference under the action of the motor, sucks the liquid stored in the external container into the liquid injection pump, and transports it to the connected liquid injection tubes through the pipeline. A plurality of liquid injection tubes are fixedly installed on the moving seat facing away from the pipetting assembly. The moving seat can reciprocate along the X-axis, Y-axis, and Z-axis directions on the base under the drive of the second power system. The liquid injection pump continuously works, pushes the sucked liquid to the liquid injection tubes through pressure, and the liquid flows out of the tube orifice along the liquid injection tubes and is accurately injected into the position where the target sample is located, such as the holes of a multi-well plate, thereby completing the liquid addition operation.

[0042] The above embodiments are only the preferred embodiments of the present invention and cannot be used to limit the scope of protection of the present invention. Any non-substantive changes and substitutions made by those skilled in the art based on the present invention fall within the scope of protection required by the present invention.

Claims

1. A multifunctional magnetic bead purification instrument, characterized in that: It includes a base, a magnetic extraction mechanism and a liquid transfer module installed on the base, and a control system for controlling the magnetic extraction mechanism and the liquid transfer module; The magnetic extraction assembly includes a magnetic rod assembly, a magnetic rod frame for fixing the magnetic rod assembly, and a first power system, wherein the first power system enables the magnetic rod assembly to reciprocate along the X-axis, Y-axis, and Z-axis directions on the base; The pipetting module comprises a pipetting component and a second power system, wherein the second power system enables the pipetting component to reciprocate along the X-axis, Y-axis and Z-axis directions on the base; A placing plate is arranged on the base, and a plurality of porous plates are arranged on the placing plate.

2. The multifunctional magnetic bead purification instrument according to claim 1, characterized in that: The magnetic bar assembly includes a plurality of magnetic bars and magnetic bar sleeves corresponding to the number of the magnetic bars, the magnetic bar frame includes a magnetic bar fixing plate and a magnetic bar sleeve fixing plate, the upper ends of the plurality of magnetic bars are fixed on the magnetic bar fixing plate; the magnetic bar sleeve fixing plate is located directly below the magnetic bar fixing plate, and the magnetic bar sleeve fixing plate is provided with through holes corresponding to the positions of the magnetic bars, the opening end of the magnetic bar sleeve is fixed at the through hole, and the magnetic bar can be inserted into the magnetic bar sleeve through the through hole under the drive of the first power system.

3. The multifunctional magnetic bead purification instrument according to claim 2, characterized in that: The first power system includes a first horizontal moving device and a first lifting device; the first horizontal moving device includes a first X-axis moving component that moves the magnetic rod assembly on the base along the X-axis direction and a Z-axis moving component that moves the magnetic rod assembly on the base along the Z-axis direction; the first lifting component includes a first lifting motor and a lifting structure that provides a lifting path for the magnetic rod assembly.

4. The multifunctional magnetic bead purification instrument according to claim 3, characterized in that: The first X-axis moving assembly includes a first driving motor, an X-axis guide rail and a first sliding seat installed on the X-axis guide rail, the first sliding seat is connected to the first driving motor, and the magnetic rod frame is installed on the first sliding seat; the lifting structure is installed on the first sliding seat and connected to the magnetic rod frame; the Z-axis moving assembly includes a second driving motor, a first ball screw, a first ball nut and a fixing frame for fixing the second driving motor, the bottom of the X-axis guide rail is fixedly connected to a mounting plate, the first ball screw is fixed to one side of the mounting plate, the first ball nut is mounted on the first ball screw, and the lower end of the mounting plate is provided with a limiting structure to prevent the first ball nut from rotating with the first ball screw, and the magnetic extraction mechanism reciprocates as a whole in the Z-axis direction under the rotation of the first ball screw through the driving line of the second driving motor.

5. The multifunctional magnetic bead purification instrument according to claim 4, characterized in that: The lifting structure includes a second ball screw and a second ball nut. The first sliding seat is provided with a receiving groove for installing the second ball screw. The upper and lower ends of the second ball screw are fixed in the receiving groove. The second ball nut is installed on the second ball screw and fixedly connected to the magnetic rod frame. A clamping groove is provided in the receiving groove along the Z-axis direction for limiting the rotation of the second ball nut, and one side of the second ball nut is clamped in the clamping groove.

6. The multifunctional magnetic bead purification instrument according to claim 4, characterized in that: The second power system includes a moving seat, a second horizontal moving device and a second lifting device; the moving seat is installed on the X-axis guide rail, the pipetting assembly is installed on the moving seat, and the second lifting device drives the pipetting assembly to reciprocate relative to the moving seat along the Y-axis direction of the base; the second horizontal moving device drives the pipetting assembly to reciprocate along the X-axis direction of the base through the moving seat, and the Z-axis moving assembly drives the pipetting assembly to reciprocate along the Z-axis direction of the base.

7. The multifunctional magnetic bead purification instrument according to claim 6, characterized in that: The pipetting assembly includes a pipetting arm, a peristaltic pump, a motor for controlling the stroke of the peristaltic pump, and an inductive sensor for identifying the liquid level in a gun tip; the pipetting arm is mounted on a movable seat and is driven by a second lifting device to reciprocate up and down relative to the movable seat; the peristaltic pump, the motor, and the inductive sensor are mounted on the pipetting arm; a connector for connecting the gun tip and the peristaltic pump is provided at the lower end of the pipetting arm.

8. The multifunctional magnetic bead purification instrument according to claim 7, characterized in that: The second lifting device includes a second lifting motor, a third ball screw and a third ball nut mounted on the ball screw. The movable seat is provided with a slide groove from top to bottom. One side of the third ball nut is installed in the slide groove. The pipetting arm is fixed on the third ball nut. The second lifting motor is connected to the third ball screw through a coupling. The connecting head is provided with a plurality of annular steps from top to bottom, and the diameters of the plurality of annular steps decrease from top to bottom for installing gun heads of different calibers.

9. The multifunctional magnetic bead purification instrument according to claim 7, characterized in that: The base is also provided with a bracket for placing a pipette tip and a gun tip withdrawal assembly, the gun tip withdrawal assembly includes a gun tip collection groove and a gun tip withdrawal rack, and the gun tip withdrawal rack is provided with an open groove matching the open end of the pipette tip.

10. The multifunctional magnetic bead purification instrument according to claim 1, characterized in that: It also includes a liquid adding module, which includes a plurality of liquid injection tubes, a liquid injection pump connected to the liquid injection tubes, a motor providing power for the liquid injection pump, and a third lifting device. The liquid injection tubes and the liquid injection pump are fixedly installed on a moving seat facing away from the pipetting assembly.

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