Shaking table device for aspergillus niger strain cultivation

Abstract

The invention relates to the field of biological engineering, in particular to a shaking table device for aspergillus niger strain cultivation, which comprises a shaking table body assembly, the rotating speed is low, a second rotating disc is jacked up together, and at the moment, a first ratchet wheel fixedly connected above the second rotating disc is matched with a second ratchet wheel, so that a driving shaft drives the second rotating disc to rotate when rotating, and the rotating disc rotates; under the limiting effect of the vibration track assembly, when the second rolling wheel drives the shake flask containing plate to vibrate in a low amplitude and the rotating speed is high, the first ratchet wheel is separated from the second ratchet wheel, the second rolling wheel is separated from the vibration track assembly, at the moment, the first rotating disc, the first rolling wheel and the first ratchet wheel fixedly connected with the first rotating disc move downwards, and the first ratchet wheel is matched with the second ratchet wheel; the first rolling wheel is in sliding connection with the vibration rail assembly, the first rotating disc rotates, the shake flask containing plate vibrates greatly through the limiting effect of the vibration rail assembly, the vibration amplitude is reduced in the spore period by changing the vibration amplitude, the spore structure is prevented from being damaged, and the germination rate is reduced.

Description

Technical Field

[0001] The present invention relates to the field of bioengineering, and specifically to a shaker device for cultivating Aspergillus niger strains. Background Art

[0002] In industries such as medicine, food, and intensive processing of agricultural products, biological transformation is a key link. Biological transformation mainly uses microorganisms or enzymes to convert low-value substances into high-value-added, highly bioactive or flavorful substances, etc. During the process of biological transformation, a large number of strains and cell lines with relevant enzyme activities or other biological activities must undergo shake flask culture to screen for high-performance strains; There is a shaker for cultivating strains with the publication number of CN107058057B, including a main body. A placement chamber is provided inside the main body. A shaker flask turntable is arranged inside the placement chamber of the main body. A fixing plate is horizontally fixed inside the placement chamber of the main body. On opposite sides of the fixing plate, an ear piece one and an ear piece two are respectively fixed. A through hole one is provided on the fixing plate. And a mounting plate is arranged below the fixing plate. A driving motor is also fixed on the lower surface of the mounting plate. The output shaft of the driving motor is vertically upward. A connecting rod is horizontally arranged at the end of the output shaft of the driving motor. The other end of the connecting rod is vertically provided with a driving shaft. The driving shaft passes through the through hole one on the fixing plate. The end of the driving shaft is fixed on the lower surface of the shaker flask turntable; A swinging mechanism capable of driving the shaker flask turntable to swing up and down is fixed on the mounting plate. The present invention has the advantage of being able to more effectively cultivate strains; The existing shakers for cultivating Aspergillus niger strains mainly have the following disadvantages: 1. In the initial stage of cultivating Aspergillus niger strains, that is, the spore stage, there is no large oxygen demand. Great vibration during the spore stage will damage the spore structure, reduce the germination rate, and destroy the synchrony. Summary of the Invention

[0003] The purpose of the present invention is to provide a shaker device for cultivating Aspergillus niger strains to solve the problems raised in the above background art.

[0004] To achieve the above invention purpose, the present invention adopts the following technical solutions: Provide a shaker device for cultivating Aspergillus niger strains, including a shaker body assembly, a driving mechanism is fixedly connected to the shaker body assembly, and a multi-amplitude adjustment mechanism is also connected to the shaker body assembly; where The driving mechanism includes: a power component, the power component is fixedly connected to the shaker body assembly, and a switching component is fixedly connected to the power component; and The multi-amplitude adjustment mechanism includes: The first amplitude adjustment component, the first amplitude adjustment component is connected to the switching component, the switching component is also connected to the second amplitude adjustment component, and both the first amplitude adjustment component and the second amplitude adjustment component are respectively connected to the upper and lower surfaces of the vibration track component; and The shaker body component includes: A bed frame, the upper end of the bed frame is connected to the shaker flask placement plate through a first spring.

[0005] Further, the power component includes: A motor, the motor is fixedly connected to the bed frame, the motor is located at the lower end of the shaker flask placement plate, a bevel gear is fixedly connected to the output end of the motor, a driving shaft is rotatably connected to the bed frame, a bevel gear is fixedly connected to the driving shaft, the two bevel gears are engaged, and the driving shaft is fixedly connected to the switching component.

[0006] Further, the switching component includes: A connector, the connector is fixedly connected to the driving shaft, one ends of two sliding guide rods are respectively fixedly connected to two sides of the connector, counterweight blocks are respectively slidably connected to the two sliding guide rods, a trapezoidal block is fixedly connected to one side of the counterweight block, and the other side of the counterweight block is fixedly connected to one end of a second spring, and the other end of the second spring is fixedly connected to the connector.

[0007] Further, the switching component also includes: A conical lifting ring, the outer side surface of the conical lifting ring abuts against the inclined surface of the trapezoidal block, both sides of the conical lifting ring are slidably connected to the guide rods, the guide rods are fixedly connected to the connecting block, and the connecting block is fixedly connected to the bed frame.

[0008] Further, the first amplitude adjustment component includes: A first turntable, the first turntable is rotatably connected to the driving shaft, the first turntable is rotatably connected to a first adjusting ring, and the first adjusting ring is connected to the conical lifting ring through a first connecting rod; and The first turntable is fixedly connected to one end of a first fixed shaft, the other end of the first fixed shaft is rotatably connected to a first roller, the first turntable is arranged at the upper end of the vibration track component, and a first ratchet is fixedly connected to the lower end of the first turntable.

[0009] Further, the second amplitude adjustment component includes: A second turntable, the second turntable is rotatably connected to the driving shaft, the second turntable is rotatably connected to a second adjusting ring, and the second adjusting ring is connected to the conical lifting ring through a second connecting rod; and The second turntable is fixedly connected to one end of the second fixed shaft. The other end of the second fixed shaft is rotatably connected to the second roller. The second turntable is arranged at the lower end of the vibration track assembly. A first ratchet is fixedly connected to the upper end of the second turntable. Two second ratchets are fixedly connected to the driving shaft.

[0010] Furthermore, the vibration track assembly: A track connection block. A first track is provided at the upper end of the track connection block, and a second track is provided at the lower end of the track connection block. The track connection block is fixedly connected to the lower end of the flask placement plate through a fixed block. The first roller is slidably connected in the first track, and the second roller is slidably connected in the second track.

[0011] Compared with the prior art, the above one or more technical solutions have the following beneficial effects: 1. When the rotational speed is low and the conical lifting ring is in the state of being lifted by the trapezoidal block, the second turntable is also lifted together, so that the second fixed shaft, the second roller, and the first ratchet are also lifted together. At this time, the second roller is slidably connected to the vibration track assembly. At this time, the first ratchet fixedly connected above the second turntable is adapted to one of the second ratchets, so that when the driving shaft rotates, it drives the second turntable to rotate. Under the limiting action of the vibration track assembly, the second roller drives the flask placement plate to vibrate with a small amplitude. When the rotational speed is high, the conical lifting ring moves downward under the action of gravity. At this time, the second turntable, the second roller, and the first ratchet fixedly connected to the second turntable move downward, and this first ratchet is disengaged from the second ratchet. The second roller disengages from the vibration track assembly. At this time, the first turntable, the first roller, and the first ratchet fixedly connected to the first turntable move downward, and this first ratchet is adapted to the second ratchet. The first roller is slidably connected to the vibration track assembly, and the first turntable rotates. Through the limiting action of the vibration track assembly, the flask placement plate vibrates with a large amplitude. By changing the vibration amplitude, during the spore period, the vibration amplitude is adjusted to be small to avoid damaging the spore structure and reducing the germination rate.

[0012] 2. When the rotational speed is low, the second spring is almost in its original length state. At this time, the conical lifting ring is in the state of being lifted by the trapezoidal block. When the rotational speed is high, the second spring is stretched. At this time, the trapezoidal block moves outward and is in a disengaged state from the conical lifting ring. The conical lifting ring moves downward along the guide rod under the action of gravity. When the conical lifting ring moves downward, due to inertia, when the conical lifting ring moves to the maximum distance and suddenly stops, there is a downward inertia at this time, causing the device to vibrate longitudinally. Combining the above vibration effects can achieve a three-dimensional vibration effect, avoiding the problem of single vibration direction, and at the same time reducing the problem of Aspergillus niger species clustering. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] The schematic diagrams in the specification, which form a part of the present invention, are used to provide a further understanding of the present invention. The illustrative embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation to the present invention.

[0014] In addition, the terms "installed", "set up", "equipped with", "connected", "linked", "socketed" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral structure; it can be a mechanical connection or an electrical connection; it can be directly connected, or indirectly connected through an intermediate medium, or there can be internal communication between two devices, components or parts. For those of ordinary skill in the art, the specific meanings of the above terms in this application can be understood according to specific circumstances.

[0015] Figure 1 It is a schematic diagram of the overall three-dimensional structure of the present invention; Figure 2 It is the front view of the overall three-dimensional structure of the present invention; Figure 3 It is a schematic diagram of the overall three-dimensional structure of the driving mechanism of the present invention; Figure 4 It is a schematic diagram of the overall three-dimensional structure of the power component of the present invention; Figure 5 It is a schematic diagram of the overall three-dimensional structure of the conical lifting ring of the present invention; Figure 6 It is the front view of the overall three-dimensional structure of the conical lifting ring of the present invention; Figure 7 It is the front view of the overall three-dimensional structure of the multi-amplitude adjustment mechanism of the present invention; Figure 8 It is a schematic diagram of the overall three-dimensional structure of the first amplitude adjustment component of the present invention; Figure 9 It is an exploded schematic diagram of the overall three-dimensional structure of the first amplitude adjustment component of the present invention; Figure 10 It is a schematic diagram of the overall three-dimensional structure of the track connection block of the present invention; Figure 11 It is the top view of the overall three-dimensional structure of the track connection block of the present invention; Figure 12 It is the bottom view of the overall three-dimensional structure of the track connection block of the present invention; Figure 13 It is a schematic diagram of the overall three-dimensional structure of the first ratchet of the present invention; In the drawings, the list of components represented by each reference numeral is as follows: 1. Shaker body assembly; 11. Bed frame; 12. First spring; 13. Shaking flask placement plate; 2. Driving mechanism; 21. Power component; 211. Motor; 212. Bevel gear; 213. Driving shaft; 22. Switching component; 221. Connector; 222. Sliding guide rod; 223. Counterweight; 224. Trapezoidal block; 225. Second spring; 226. Conical lifting ring; 227. Guide rod; 228. Connection block; 3. Multi - amplitude adjustment mechanism; 31. First amplitude adjustment component; 311. First turntable; 312. First adjusting ring; 313. First connecting rod; 314. First fixed shaft; 315. First roller; 316. First ratchet; 32. Second amplitude adjustment component; 321. Second turntable; 322. Second adjusting ring; 323. Second connecting rod; 324. Second fixed shaft; 325. Second roller; 326. Second ratchet; 33. Vibration track component; 331. Track connection block; 332. First track; 333. Second track; 334. Fixed block. Detailed implementation manner

[0016] In order to enable those skilled in the art to better understand the solution of this application, the following will clearly and completely describe the technical solutions in the embodiments of this application with reference to the accompanying drawings in the embodiments of this application. Obviously, the described embodiments are only a part of the embodiments of this application, rather than all of the embodiments. Based on the embodiments in this application, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of this application.

[0017] Refer to Figures 1-3 As shown in and 7, a shaker device for cultivating Aspergillus niger species is characterized in that: It includes a shaker body assembly 1, a driving mechanism 2 is fixedly connected to the shaker body assembly 1, and a multi - amplitude adjustment mechanism 3 is also connected to the shaker body assembly 1; where The driving mechanism 2 includes: A power component 21, the power component 21 is fixedly connected to the shaker body assembly 1, and a switching component 22 is fixedly connected to the power component 21; and The multi - amplitude adjustment mechanism 3 includes: A first amplitude adjustment component 31, the first amplitude adjustment component 31 is connected to the switching component 22, the switching component 22 is also connected to a second amplitude adjustment component 32, and both the first amplitude adjustment component 31 and the second amplitude adjustment component 32 are respectively connected to the upper and lower surfaces of the vibration track component 33; and The shaker body assembly 1 includes: A bed frame 11, the upper end of the bed frame 11 is connected to a shaker flask placement plate 13 through a first spring 12.

[0018] Fix the shaker flask on the shaker flask placement plate 13. By starting the power component 21, the power component 21 drives the switching component 22 to rotate. When the rotation speed of the power component 21 is low, through the limiting effect of the vibration track component 33, the second amplitude adjustment component 32 rotates, thereby driving the shaker flask placement plate 13 to shake. When the rotation speed is high, through the action of the switching component 22, the first amplitude adjustment component 31 drives the shaker flask placement plate 13 to shake.

[0019] Refer to Figure 4 As shown, the power assembly 21 includes: A motor 211, which is fixedly connected to the bed frame 11. The motor 211 is located at the lower end of the flask placing plate 13. A bevel gear 212 is fixedly connected to the output end of the motor 211. A driving shaft 213 is rotatably connected to the bed frame 11. A bevel gear 212 is fixedly connected to the driving shaft 213. The two bevel gears 212 are engaged, and the driving shaft 213 is fixedly connected to the switching assembly 22.

[0020] By rotating the motor 211, the motor 211 drives a bevel gear 212 fixedly connected thereto to rotate. Through the meshing of the two bevel gears 212, the driving shaft 213 is driven to rotate. By rotating the driving shaft 213, the switching assembly 22 is driven to rotate.

[0021] Refer to Figures 5-6 As shown, the switching assembly 22 includes: A connector 221, which is fixedly connected to the driving shaft 213. One end of each of two sliding guide rods 222 is fixedly connected to both sides of the connector 221. A counterweight 223 is slidably connected to each of the two sliding guide rods 222. A trapezoidal block 224 is fixedly connected to one side of the counterweight 223. The other side of the counterweight 223 is fixedly connected to one end of a second spring 225. The other end of the second spring 225 is fixedly connected to the connector 221.

[0022] By rotating the driving shaft 213, the connector 221 is driven to rotate, and then the sliding guide rods 222 rotate together. When the rotational speed is low, the centripetal force required for the counterweight 223 and the trapezoidal block 224 is small and is not sufficient to stretch the second spring 225 too much. When the rotational speed is high, the centripetal force required for the counterweight 223 and the trapezoidal block 224 is large. At this time, the second spring 225 is stretched, and the counterweight 223 and the trapezoidal block 224 move outward.

[0023] The switching assembly 22 further includes: A conical lifting ring 226, the outer side surface of which abuts against the inclined surface of the trapezoidal block 224. The two sides of the conical lifting ring 226 are slidably connected to guide rods 227. The guide rods 227 are fixedly connected to a connecting block 228. The connecting block 228 is fixedly connected to the bed frame 11.

[0024] When the rotational speed is low, the second spring 225 is almost in its original length state. At this time, the conical lifting ring 226 is in a state of being lifted by the trapezoidal block 224. When the rotational speed is high, the second spring 225 is stretched. At this time, the trapezoidal block 224 moves outward and is disengaged from the conical lifting ring 226. The conical lifting ring 226 moves downward along the guide rod 227 under the action of gravity.

[0025] Referring to Figures 8-9 as shown in FIGS. 13, the first amplitude adjustment assembly 31 includes: a first turntable 311 rotatably connected to the driving shaft 213. The first turntable 311 is rotatably connected to an adjustment ring 312. The adjustment ring 312 is connected to the conical lifting ring 226 through a connecting rod 313; and one end of the first turntable 311 is fixedly connected to one end of a fixed shaft 314. The other end of the fixed shaft 314 is rotatably connected to a first roller 315. The first turntable 311 is disposed at the upper end of the vibration track assembly 33. A first ratchet 316 is fixedly connected to the lower end of the first turntable 311.

[0026] When the rotational speed is low and the conical lifting ring 226 is in a state of being lifted by the trapezoidal block 224, the first turntable 311 is also lifted, so that the fixed shaft 314, the first roller 315 and the first ratchet 316 are also lifted together. At this time, the first roller 315 is disengaged from the vibration track assembly 33.

[0027] The second amplitude adjustment assembly 32 includes: a second turntable 321 rotatably connected to the driving shaft 213. The second turntable 321 is rotatably connected to an adjustment ring 322. The adjustment ring 322 is connected to the conical lifting ring 226 through a connecting rod 323; and one end of the second turntable 321 is fixedly connected to one end of a fixed shaft 324. The other end of the fixed shaft 324 is rotatably connected to a second roller 325. The second turntable 321 is disposed at the lower end of the vibration track assembly 33. A first ratchet 316 is fixedly connected to the upper end of the second turntable 321. Two second ratchets 326 are fixedly connected to the driving shaft 213.

[0028] When the rotational speed is low and the conical lifting ring 226 is in the state of being lifted by the trapezoidal block 224, the second turntable 321 is also lifted together, so that the second fixed shaft 324, the second roller 325 and the first ratchet wheel 316 are also lifted together. At this time, the second roller 325 is slidably connected to the vibration track assembly 33. At this time, the first ratchet wheel 316 fixedly connected above the second turntable 321 is adapted to a second ratchet wheel 326, so that when the driving shaft 213 rotates, it drives the second turntable 321 to rotate. Under the limiting action of the vibration track assembly 33, the second roller 325 drives the flask placing plate 13 to vibrate with a small amplitude. When the rotational speed is high, the conical lifting ring 226 moves downward under the action of gravity. At this time, the second turntable 321, the second roller 325 and the first ratchet wheel 316 fixedly connected to the second turntable 321 move downward, and the first ratchet wheel 316 is disengaged from the second ratchet wheel 326, and the second roller 325 is disengaged from the vibration track assembly 33. At this time, the first turntable 311, the first roller 315 and the first ratchet wheel 316 fixedly connected to the first turntable 311 move downward, the first ratchet wheel 316 is adapted to the second ratchet wheel 326, the first roller 315 is slidably connected to the vibration track assembly 33, and the first turntable 311 rotates. Through the limiting action of the vibration track assembly 33, the flask placing plate 13 vibrates with a large amplitude.

[0029] Referring to Figures 10-12 as shown, the vibration track assembly 33: A track connecting block 331, the upper end of the track connecting block 331 is provided with a first track 332, the lower end of the track connecting block 331 is provided with a second track 333, the track connecting block 331 is fixedly connected to the lower end of the flask placing plate 13 through a fixing block 334, the first roller 315 is slidably connected in the first track 332, and the second roller 325 is slidably connected in the second track 333.

[0030] The first roller 315 is slidably connected in the first track 332, the second roller 325 is slidably connected in the second track 333, and the variation range of the first track 332 is greater than that of the second track 333.

[0031] In the description of the present invention, it should be understood that the orientation or positional relationships indicated by the terms "longitudinal", "transverse", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. are based on the orientation or positional relationships shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as a limitation to the present invention. In the description of the present invention, unless otherwise specified and defined, it should be noted that the terms "mounted", "connected", "coupled" should be understood in a broad sense. For example, it may be a mechanical connection or an electrical connection, or may be the communication inside two elements. It may be directly connected or indirectly connected through an intermediate medium. For those of ordinary skill in the art, the specific meanings of the above terms can be understood according to specific circumstances.

[0032] The above are only the preferred embodiments of the present invention, and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included within the protection scope of the present invention.

Claims

1. A shaking table device for cultivating Aspergillus niger, characterized in that: The invention comprises a rocking table body component (1), to which a driving mechanism (2) is fixedly connected, and a multi-amplitude adjustment mechanism (3) is also connected to the rocking table body component (1); wherein The driving mechanism (2) comprises: A power assembly (21), the power assembly (21) being fixedly connected to the rocking table body assembly (1), and a switching assembly (22) being fixedly connected to the power assembly (21); and The multi-amplitude adjustment mechanism (3) comprises: a first amplitude adjustment component (31), the first amplitude adjustment component (31) being connected to the switching component (22), the switching component (22) being further connected to the second amplitude adjustment component (32), the first amplitude adjustment component (31) and the second amplitude adjustment component (32) being respectively connected to upper and lower surfaces of the vibration track component (33); and The rocking table body component (1) comprises: A bed frame (11), wherein the upper end of the bed frame (11) is connected to a shaking bottle placement plate (13) via a spring 1 (12).

2. The shaking table device for cultivating Aspergillus niger species according to claim 1, characterized in that: The power assembly (21) comprises: A motor (211) is fixedly connected to the bed frame (11), the motor (211) is located at the lower end of the shaking bottle placement plate (13), an output end of the motor (211) is fixedly connected to a bevel gear (212), a driving shaft (213) is rotatably connected to the bed frame (11), a bevel gear (212) is fixedly connected to the driving shaft (213), the two bevel gears (212) are meshed, and the driving shaft (213) is fixedly connected to the switching component (22).

3. A shaking table device for cultivating Aspergillus niger species according to claim 2, characterized in that: The switching component (22) comprises: A connector (221), the connector (221) being fixedly connected to the driving shaft (213), the two sides of the connector (221) being fixedly connected to one end of two sliding guide rods (222), the two sliding guide rods (222) being slidably connected to counterweight blocks (223), one side of the counterweight block (223) being fixedly connected to a trapezoidal block (224), the other side of the counterweight block (223) being fixedly connected to one end of a second spring (225), and the other end of the second spring (225) being fixedly connected to the connector (221).

4. The shaking table device for cultivating Aspergillus niger species according to claim 3, characterized in that: The switching component (22) further comprises: A conical lifting ring (226), the outer side surface of the conical lifting ring (226) abuts against the inclined surface of the trapezoidal block (224), both sides of the conical lifting ring (226) are slidably connected to the guide rod (227), the guide rod (227) is fixedly connected to the connecting block (228), and the connecting block (228) is fixedly connected to the bed frame (11).

5. The shaking table device for cultivating Aspergillus niger species according to claim 4, characterized in that: The first amplitude adjustment component (31) comprises: A rotating disk (311), wherein the rotating disk (311) is rotatably connected to the driving shaft (213), the rotating disk (311) is rotatably connected to an adjusting ring (312), and the adjusting ring (312) is connected to a conical lifting ring (226) via a connecting rod (313); and The turntable 1 (311) is fixedly connected to one end of a fixed shaft 1 (314), and the other end of the fixed shaft 1 (314) is rotatably connected to a roller 1 (315). The turntable 1 (311) is arranged at the upper end of the vibration track assembly (33), and a ratchet 1 (316) is fixedly connected to the lower end of the turntable 1 (311).

6. The shaking table device for cultivating Aspergillus niger species according to claim 5, characterized in that: The second amplitude adjustment component (32) comprises: A second rotating disk (321), the second rotating disk (321) being rotatably connected to the driving shaft (213), the second rotating disk (321) being rotatably connected to a second adjusting ring (322), and the second adjusting ring (322) being connected to a conical lifting ring (226) via a second connecting rod (323); and The second turntable (321) is fixedly connected to one end of the second fixed shaft (324), and the other end of the second fixed shaft (324) is rotatably connected to the second roller (325). The second turntable (321) is arranged at the lower end of the vibration track assembly (33), and the upper end of the second turntable (321) is fixedly connected to a ratchet wheel (316), and the driving shaft (213) is fixedly connected to two second ratchet wheels (326).

7. The shaking table device for cultivating Aspergillus niger species according to claim 6, characterized in that: The vibration track assembly (33): A track connection block (331), wherein the upper end of the track connection block (331) is provided with a track 1 (332), and the lower end of the track connection block (331) is provided with a track 2 (333). The track connection block (331) is fixedly connected to the lower end of the shaking bottle placement plate (13) via a fixing block (334), the roller 1 (315) is slidably connected in the track 1 (332), and the roller 2 (325) is slidably connected in the track 2 (333).

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