A shaking table device for cultivating Aspergillus niger strains

Abstract

This invention relates to the field of bioengineering, specifically to a shaking incubator device for cultivating Aspergillus niger. The device includes a shaking incubator body assembly. At low speeds, a second rotating disc is also lifted. At this time, a ratchet wheel 1 fixedly connected above the second rotating disc is adapted to a second ratchet wheel, causing the drive shaft to rotate and drive the second rotating disc. Under the limiting action of the vibration track assembly, the second roller causes the shaker flask placement plate to vibrate at a low amplitude. At high speeds, the ratchet wheel 1 disengages from the second ratchet wheel, and the second roller disengages from the vibration track assembly. At this time, the first rotating disc, the first roller, and the ratchet wheel 1 fixedly connected to the first rotating disc move downwards. The ratchet wheel 1 is adapted to the second ratchet wheel, and the first roller is slidably connected to the vibration track assembly. The first rotating disc rotates, and through the limiting action of the vibration track assembly, the shaker flask placement plate vibrates significantly. By changing the vibration amplitude, during the spore stage, the vibration amplitude can be reduced to avoid damaging the spore structure and reducing the germination rate.

Description

Technical Field

[0001] This invention relates to the field of bioengineering, specifically to a shaking incubator for cultivating Aspergillus niger. Background Technology

[0002] In industries such as pharmaceuticals, food, and the intensive processing of agricultural products, biotransformation is a crucial step. Biotransformation primarily uses microorganisms or enzymes to convert low-value substances into high-value, highly bioactive, or flavorful substances. During biotransformation, numerous strains and cell lines with relevant enzyme activity or other biological activities must undergo shake-flask culture to screen for high-performance strains.

[0003] A microbial culture shaker, with publication number CN107058057B, includes a main body with a placement chamber inside. A shaker disc is placed inside the placement chamber. A fixed plate is horizontally fixed inside the placement chamber. Ear pieces one and two are fixed to opposite sides of the fixed plate, respectively. A through hole is formed on the fixed plate, and a mounting plate is located below the fixed plate. A drive motor is fixed to the lower surface of the mounting plate. The output shaft of the drive motor is vertically upward. A connecting rod is horizontally attached to the end of the output shaft, and a drive shaft is vertically attached to the other end of the connecting rod. The drive shaft passes through the through hole in the fixed plate, and its end is fixed to the lower surface of the shaker disc. A oscillating mechanism capable of driving the shaker disc to oscillate up and down is fixed to the mounting plate. This invention has the advantage of more effectively culturing microorganisms.

[0004] The existing shakers for cultivating Aspergillus niger strains have the following main drawbacks:

[0005] 1. In the early stage of cultivation, i.e. the spore stage, Aspergillus niger does not have a large oxygen demand. Large vibrations during the spore stage will damage the spore structure, reduce the germination rate and disrupt synchronization. Summary of the Invention

[0006] The purpose of this invention is to provide a shaking incubator device for cultivating Aspergillus niger, so as to solve the problems mentioned in the background art.

[0007] To achieve the above-mentioned objectives, the present invention adopts the following technical solution:

[0008] A shaking incubator device for cultivating Aspergillus niger strains is provided.

[0009] The system includes a shaking table body assembly, on which a drive mechanism is fixedly connected, and a multi-amplitude adjustment mechanism is also connected; wherein...

[0010] The driving mechanism includes:

[0011] A power assembly, fixedly connected to the shaking table body assembly, and a switching component fixedly connected to the power assembly; and

[0012] The multi-amplitude adjustment mechanism includes:

[0013] A first amplitude adjustment component is connected to a switching component, and the switching component is also connected to a second amplitude adjustment component. Both the first and second amplitude adjustment components are connected to the upper and lower surfaces of the vibration track component, respectively.

[0014] The shaker body assembly includes:

[0015] The bed frame, the upper end of which is connected to the shaker plate by a spring.

[0016] Furthermore, the power assembly includes:

[0017] The motor is fixedly connected to the bed frame and is located at the lower end of the shaker plate. A bevel gear is fixedly connected to the output end of the motor. A drive shaft is rotatably connected to the bed frame, and a bevel gear is fixedly connected to the drive shaft. The two bevel gears mesh, and the drive shaft is fixedly connected to the switching assembly.

[0018] Furthermore, the switching component includes:

[0019] The connector is fixedly connected to the drive shaft. Both sides of the connector are fixedly connected to one end of two sliding guide rods. A counterweight is slidably connected to each of the two sliding guide rods. A trapezoidal block is fixedly connected to one side of the counterweight. The other side of the counterweight is fixedly connected to one end of a second spring. The other end of the second spring is fixedly connected to the connector.

[0020] Furthermore, the switching component also includes:

[0021] A conical lifting ring, the outer side of which abuts against the inclined surface of a trapezoidal block, the two sides of which are slidably connected to guide rods, the guide rods being fixedly connected to connecting blocks, and the connecting blocks being fixedly connected to the bed frame.

[0022] Furthermore, the first amplitude adjustment component includes:

[0023] Turntable 1, rotatably connected to the drive shaft, turntable 1 rotatably connected to adjusting ring 1, adjusting ring 1 being connected to a conical lifting ring via a connecting rod 1; and

[0024] One end of the turntable is fixedly connected to one end of the fixed shaft, and the other end of the fixed shaft is rotatably connected to one roller. The turntable is located at the upper end of the vibration track assembly, and a ratchet is fixedly connected to the lower end of the turntable.

[0025] Furthermore, the second amplitude adjustment component includes:

[0026] Turntable two, rotatably connected to the drive shaft, turntable two and adjusting ring two, adjusting ring two being connected to a conical lifting ring via connecting rod two; and

[0027] The turntable 2 is fixedly connected to one end of the fixed shaft 2, and the other end of the fixed shaft 2 is rotatably connected to the roller 2. The turntable 2 is located at the lower end of the vibration track assembly, and a ratchet 1 is fixedly connected to the upper end of the turntable 2. Two ratchet 2s are fixedly connected to the drive shaft.

[0028] Furthermore, the vibration track assembly:

[0029] The track connecting block has a track one at its upper end and a track two at its lower end. The track connecting block is fixedly connected to the lower end of the shaker placement plate by a fixing block. Roller one is slidably connected in track one and roller two is slidably connected in track two.

[0030] Compared with existing technologies, one or more of the above technical solutions have the following beneficial effects:

[0031] 1. At low speeds, when the conical lifting ring is lifted by the trapezoidal block, turntable two is also lifted, causing fixed shaft two, roller two, and ratchet one to be lifted as well. At this time, roller two is slidably connected to the vibration track assembly. Ratchet one, fixedly connected above turntable two, is matched with ratchet two, causing the drive shaft to rotate and drive turntable two to rotate. Under the limiting action of the vibration track assembly, roller two causes the shaker bottle placement plate to vibrate at a low amplitude. At high speeds, the conical lifting ring moves downwards under gravity. Turntable 2, roller 2, and ratchet fixedly connected to turntable 2 move down, and ratchet 1 disengages from ratchet 2. Roller 2 disengages from the vibration track assembly. At this time, turntable 1, roller 1, and ratchet 1 fixedly connected to turntable 1 move down. Ratchet 1 and ratchet 2 are adapted to each other. Roller 1 is slidably connected to the vibration track assembly. Turntable 1 rotates. Through the limiting effect of the vibration track assembly, the shaker placement plate vibrates significantly. By changing the vibration amplitude, during the spore stage, the vibration amplitude is reduced to avoid damaging the spore structure and reducing the germination rate.

[0032] 2. At low speeds, spring two is almost at its original length. At this time, the conical lifting ring is lifted by the trapezoidal block. At high speeds, spring two is stretched. At this time, the trapezoidal block moves outward and is disengaged from the conical lifting ring. The conical lifting ring moves downward along the guide rod under the action of gravity. When the conical lifting ring moves downward, due to inertia, it suddenly stops when it reaches its maximum distance. At this time, there is a downward inertia, which causes the device to vibrate longitudinally. Combined with the above vibration effect, a three-dimensional vibration effect can be achieved, avoiding the problem of a single vibration direction. At the same time, it can reduce the problem of Aspergillus niger clumps. Attached Figure Description

[0033] The accompanying drawings, which form part of this invention, are used to provide a further understanding of the invention. The illustrative embodiments of the invention and their descriptions are used to explain the invention and do not constitute an improper limitation of the invention.

[0034] Furthermore, the terms "installation," "setup," "equipped with," "connection," "linking," and "socketing" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral structure; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium, or an internal connection between two devices, components, or parts. Those skilled in the art can understand the specific meaning of these terms in this application based on the specific circumstances.

[0035] Figure 1 This is a schematic diagram of the overall three-dimensional structure of the present invention;

[0036] Figure 2 This is a front view of the overall three-dimensional structure of the present invention;

[0037] Figure 3 This is a schematic diagram of the overall three-dimensional structure of the drive mechanism of the present invention;

[0038] Figure 4 This is a schematic diagram of the overall three-dimensional structure of the power component of the present invention;

[0039] Figure 5 This is a schematic diagram of the overall three-dimensional structure of the conical lifting ring of the present invention;

[0040] Figure 6 This is a front view of the overall three-dimensional structure of the conical lifting ring of the present invention;

[0041] Figure 7 This is a front view of the overall three-dimensional structure of the multi-amplitude adjustment mechanism of the present invention;

[0042] Figure 8 This is a schematic diagram of the overall three-dimensional structure of the first amplitude adjustment component of the present invention;

[0043] Figure 9This is an exploded three-dimensional structural diagram of the first amplitude adjustment component of the present invention;

[0044] Figure 10 This is a schematic diagram of the overall three-dimensional structure of the track connecting block of the present invention;

[0045] Figure 11 This is a top view of the overall three-dimensional structure of the track connecting block of the present invention;

[0046] Figure 12 This is a bottom view of the overall three-dimensional structure of the track connecting block of the present invention;

[0047] Figure 13 This is a schematic diagram of the integral three-dimensional structure of the ratchet of the present invention;

[0048] The attached diagram lists the components represented by each number as follows:

[0049] 1. Shaking table body assembly; 11. Bed frame; 12. Spring 1; 13. Shaking bottle placement plate;

[0050] 2. Drive mechanism; 21. Power assembly; 211. Motor; 212. Bevel gear; 213. Drive shaft; 22. Switching assembly; 221. Connector; 222. Sliding guide rod; 223. Counterweight; 224. Trapezoidal block; 225. Spring II; 226. Conical lifting ring; 227. Guide rod; 228. Connecting block;

[0051] 3. Multi-amplitude adjustment mechanism; 31. First amplitude adjustment component; 311. Turntable 1; 312. Adjusting ring 1; 313. Connecting rod 1; 314. Fixed shaft 1; 315. Roller 1; 316. Ratchet 1; 32. Second amplitude adjustment component; 321. Turntable 2; 322. Adjusting ring 2; 323. Connecting rod 2; 324. Fixed shaft 2; 325. Roller 2; 326. Ratchet 2; 33. Vibration track assembly; 331. Track connecting block; 332. Track 1; 333. Track 2; 334. Fixed block. Detailed Implementation

[0052] To enable those skilled in the art to better understand the present application, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present application, and not all embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative effort should fall within the scope of protection of the present application.

[0053] Reference Figure 1-3 As shown in Figure 7, a shaking table device for cultivating Aspergillus niger is characterized by:

[0054] The system includes a shaking table body assembly 1, on which a drive mechanism 2 is fixedly connected, and a multi-amplitude adjustment mechanism 3 is also connected; wherein...

[0055] The drive mechanism 2 includes:

[0056] Power assembly 21, which is fixedly connected to the shaking table body assembly 1, and a switching assembly 22 is fixedly connected to the power assembly 21; and

[0057] The multi-amplitude adjustment mechanism 3 includes:

[0058] A first amplitude adjustment component 31 is connected to a switching component 22, which is also connected to a second amplitude adjustment component 32. Both the first and second amplitude adjustment components 31 and 32 are connected to the upper and lower surfaces of the vibration track component 33, respectively.

[0059] The shaking table body assembly 1 includes:

[0060] The upper end of the bed frame 11 is connected to the shaker plate 13 by a spring 12.

[0061] The shaker is fixed on the shaker placement plate 13. By activating the power component 21, the power component 21 drives the switching component 22 to rotate. When the speed of the power component 21 is low, the second amplitude adjustment component 32 rotates due to the limiting effect of the vibration track component 33, thereby driving the shaker placement plate 13 to shake. When the speed is high, the first amplitude adjustment component 31 drives the shaker placement plate 13 to shake due to the action of the switching component 22.

[0062] Reference Figure 4 As shown, the power assembly 21 includes:

[0063] Motor 211 is fixedly connected to bed frame 11. Motor 211 is located at the lower end of shaker plate 13. A bevel gear 212 is fixedly connected to the output end of motor 211. A drive shaft 213 is rotatably connected to bed frame 11. A bevel gear 212 is fixedly connected to drive shaft 213. The two bevel gears 212 mesh. Drive shaft 213 is fixedly connected to switching assembly 22.

[0064] The rotation of motor 211 causes a bevel gear 212 fixedly connected to it to rotate. The meshing of the two bevel gears 212 drives the drive shaft 213 to rotate, and the rotation of the drive shaft 213 drives the switching component 22 to rotate.

[0065] Reference Figure 5-6 As shown, the switching component 22 includes:

[0066] Connector 221 is fixedly connected to drive shaft 213. Both sides of connector 221 are fixedly connected to one end of two sliding guide rods 222. Counterweights 223 are slidably connected to the two sliding guide rods 222. A trapezoidal block 224 is fixedly connected to one side of the counterweight 223. The other side of the counterweight 223 is fixedly connected to one end of spring 225. The other end of spring 225 is fixedly connected to connector 221.

[0067] The rotation of the drive shaft 213 drives the connector 221 to rotate, which in turn causes the sliding guide rod 222 to rotate together. When the speed is low, the centripetal force required by the counterweight 223 and the trapezoidal block 224 is small, which is insufficient to stretch the second spring 225 too much. When the speed is high, the centripetal force required by the counterweight 223 and the trapezoidal block 224 is large. At this time, the second spring 225 is stretched, and the counterweight 223 and the trapezoidal block 224 move outward.

[0068] The switching component 22 further includes:

[0069] A conical lifting ring 226 is provided, the outer side of which abuts against the inclined surface of the trapezoidal block 224. The two sides of the conical lifting ring 226 are slidably connected to the guide rod 227. The guide rod 227 is fixedly connected to the connecting block 228, and the connecting block 228 is fixedly connected to the bed frame 11.

[0070] At low speeds, spring 225 is almost at its original length. At this time, the conical lifting ring 226 is lifted by the trapezoidal block 224. At high speeds, spring 225 is stretched. At this time, the trapezoidal block 224 moves outward and is detached from the conical lifting ring 226. The conical lifting ring 226 moves down along the guide rod 227 under the action of gravity.

[0071] Reference Figure 8-9 As shown in Figure 13, the first amplitude adjustment component 31 includes:

[0072] Turntable 311, rotatably connected to drive shaft 213, and rotatably connected to adjusting ring 312, which is connected to conical lifting ring 226 via connecting rod 313; and

[0073] The turntable 311 is fixedly connected to one end of the fixed shaft 314, and the other end of the fixed shaft 314 is rotatably connected to the roller 315. The turntable 311 is located at the upper end of the vibration track assembly 33, and a ratchet 316 is fixedly connected to the lower end of the turntable 311.

[0074] When the rotation speed is low and the conical lifting ring 226 is in a state of being lifted by the trapezoidal block 224, the turntable 311 is also lifted, thereby causing the fixed shaft 314, roller 315 and ratchet 316 to be lifted as well. At this time, the roller 315 is disengaged from the vibration track assembly 33.

[0075] The second amplitude adjustment component 32 includes:

[0076] Turntable 321, rotatably connected to drive shaft 213, and rotatably connected to adjusting ring 322, which is connected to conical lifting ring 226 via connecting rod 323; and

[0077] The turntable 321 is fixedly connected to one end of the fixed shaft 324, and the other end of the fixed shaft 324 is rotatably connected to the roller 325. The turntable 321 is located at the lower end of the vibration track assembly 33. A ratchet 316 is fixedly connected to the upper end of the turntable 321, and two ratchet 326s are fixedly connected to the drive shaft 213.

[0078] When the rotational speed is low and the conical lifting ring 226 is lifted by the trapezoidal block 224, the turntable 321 is also lifted, causing the fixed shaft 324, roller 325, and ratchet 316 to be lifted as well. At this time, roller 325 is slidably connected to the vibration track assembly 33. The ratchet 316 fixedly connected above the turntable 321 is matched with one ratchet 326, so that the drive shaft 213 drives the turntable 321 to rotate when it rotates. Under the limiting action of the vibration track assembly 33, roller 325 causes the shaker bottle placement plate 13 to vibrate at a low amplitude. When the rotational speed is high, the conical lifting ring 226 is lifted by the trapezoidal block 224. Ring 226 moves downward under the action of gravity. At this time, turntable 221, roller 225 and ratchet 1 316 fixedly connected to turntable 221 move downward and ratchet 1 316 disengages from ratchet 226. Roller 225 disengages from vibration track assembly 33. At this time, turntable 1 311, roller 1 315 and ratchet 1 316 fixedly connected to turntable 1 311 move downward. Ratchet 1 316 is adapted to ratchet 226. Roller 1 315 is slidably connected to vibration track assembly 33. Turntable 1 311 rotates. Through the limiting action of vibration track assembly 33, the shaker placement plate 13 vibrates significantly.

[0079] Reference Figure 10-12 As shown, the vibration track assembly 33:

[0080] The track connecting block 331 has a track 1 332 at its upper end and a track 2 333 at its lower end. The track connecting block 331 is fixedly connected to the lower end of the shaker placement plate 13 by a fixing block 334. The roller 1 315 is slidably connected in the track 1 332 and the roller 2 325 is slidably connected in the track 2 333.

[0081] Roller 1 315 is slidably connected within track 1 332, and roller 2 325 is slidably connected within track 2 333. The range of change of track 1 332 is greater than that of track 2 333.

[0082] In the description of this invention, it should be understood that the terms "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," and "outer," etc., indicating orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, are only for the convenience of describing the invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the invention. In the description of this invention, unless otherwise specified and limited, it should be noted that the terms "installed," "connected," and "linked" should be interpreted broadly. For example, they can refer to mechanical or electrical connections, or internal connections between two elements; they can be direct connections or indirect connections through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms according to the specific circumstances.

[0083] The above are merely preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A shaking incubator for cultivating Aspergillus niger, characterized in that: The system includes a shaking table body assembly (1), on which a drive mechanism (2) is fixedly connected, and on which a multi-amplitude adjustment mechanism (3) is also connected; wherein The drive mechanism (2) includes: Power assembly (21), which is fixedly connected to the rocker body assembly (1), and a switching assembly (22) is fixedly connected to the power assembly (21); and The multi-amplitude adjustment mechanism (3) includes: A first amplitude adjustment component (31) is connected to a switching component (22), which is also connected to a second amplitude adjustment component (32). Both the first amplitude adjustment component (31) and the second amplitude adjustment component (32) are connected to the upper and lower surfaces of the vibration track component (33), respectively. The shaker body assembly (1) includes: Bed frame (11), the upper end of which is connected to the shaker plate (13) by spring one (12); The switching component (22) includes: Connector (221), the connector (221) is fixedly connected to the drive shaft (213), the two sides of the connector (221) are respectively fixedly connected to one end of two sliding guide rods (222), the two sliding guide rods (222) are respectively slidably connected to counterweights (223), one side of the counterweight (223) is fixedly connected to a trapezoidal block (224), the other side of the counterweight (223) is fixedly connected to one end of spring two (225), and the other end of spring two (225) is fixedly connected to connector (221); The switching component (22) also includes: A conical lifting ring (226) is formed, the outer side of which abuts against the inclined surface of the trapezoidal block (224). The two sides of the conical lifting ring (226) are slidably connected to the guide rod (227). The guide rod (227) is fixedly connected to the connecting block (228). The connecting block (228) is fixedly connected to the bed frame (11). The first amplitude adjustment component (31) includes: Turntable 1 (311), which is rotatably connected to the drive shaft (213), is rotatably connected to adjusting ring 1 (312), and adjusting ring 1 (312) is connected to conical lifting ring (226) via connecting rod 1 (313); and The turntable (311) is fixedly connected to one end of the fixed shaft (314), and the other end of the fixed shaft (314) is rotatably connected to the roller (315). The turntable (311) is located at the upper end of the vibration track assembly (33), and a ratchet (316) is fixedly connected to the lower end of the turntable (311). The second amplitude adjustment component (32) includes: Turntable 2 (321), which is rotatably connected to the drive shaft (213), is rotatably connected to adjusting ring 2 (322), and adjusting ring 2 (322) is connected to conical lifting ring (226) via connecting rod 2 (323); and The turntable 2 (321) is fixedly connected to one end of the fixed shaft 2 (324), and the other end of the fixed shaft 2 (324) is rotatably connected to the roller 2 (325). The turntable 2 (321) is located at the lower end of the vibration track assembly (33). A ratchet 1 (316) is fixedly connected to the upper end of the turntable 2 (321), and two ratchet 2 (326) are fixedly connected to the drive shaft (213). The vibration track assembly (33) includes: The track connecting block (331) has a track one (332) at its upper end and a track two (333) at its lower end. The track connecting block (331) is fixedly connected to the lower end of the shaker placement plate (13) by a fixing block (334). The roller one (315) is slidably connected in the track one (332) and the roller two (325) is slidably connected in the track two (333).

2. The shaking incubator device for cultivating Aspergillus niger according to claim 1, characterized in that: The power assembly (21) includes: The motor (211) is fixedly connected to the bed frame (11). The motor (211) is located at the lower end of the shaker plate (13). A bevel gear (212) is fixedly connected to the output end of the motor (211). A drive shaft (213) is rotatably connected to the bed frame (11). Another bevel gear is fixedly connected to the drive shaft (213). The two bevel gears mesh. The drive shaft (213) is fixedly connected to the switching assembly (22).

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