Cell genetic experiment incubator
By designing a cell genetic experimental incubator with multiple independent culture chambers, combining automated control and environmental parameter adjustment, the problem of difficult control of a single culture dish environment is solved, and the stability and efficiency of the culture process are achieved.
Patent Information
- Application Number
- CN202510320664.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-18
- Publication Date
- 2025-06-13
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
In the prior art, the culture environment of a single culture dish is difficult to control, there is a lack of comparison observation, and it is easily disturbed by external environment during the culture process, which increases the risk of secondary infection.
A cell genetic experiment incubator was designed, which contained multiple independent culture chambers, equipped with temperature control tubes, sample delivery tubes, water mist spray tubes, vacuum extraction tubes and gas delivery tubes, and automated control and environmental parameter adjustments are achieved through the ECU core controller.
Real-time observation of culture samples and real-time monitoring and regulation of environmental parameters are achieved, reducing external interference and secondary infection risks, and improving the stability and success rate of cell culture.
Smart Images

Figure CN120137778A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of incubators, and in particular to a cell genetic experiment incubator. Background Art
[0002] Cell genetics refers to the genetic transmission phenomenon of the genetic material inside cells during cell division. Cells are the basic units of organisms and the most basic carriers of genetic information. They contain genetic material DNA inside. Through cell division, genetic information is transmitted to daughter cells, thus ensuring the transmission and stability of genetic information. Cell genetics is an important aspect of biological genetics research and is of great significance for us to understand biological problems such as genetic laws, genetic variation, and evolution. In cell genetic experiments, experimental results need to be obtained through cell culture. During the experiment, an incubator is required to store the culture dishes containing cells, and the incubator provides a suitable growth environment for the cells. It is difficult to control the culture environment of a single culture dish, and there is a lack of comparative observation. For this reason, we propose a cell genetic experiment incubator. Summary of the Invention
[0003] The purpose of the present invention is to provide a cell genetic experiment incubator to overcome the technical problems existing in the prior art.
[0004] To achieve the above technical purpose and reach the above technical effect, the present invention provides the following technical solution:
[0005] A cell genetic experiment incubator includes a frame box body. The four corners of the bottom of the frame box body are connected with pedestals. A distilled water filling port and a sample collection port are opened at the top of the frame box body. A plurality of culture chambers are arranged on the front side of the inner cavity of the frame box body. A sealing door is hinged to the front end of the culture chamber. A signboard and a door handle are connected to the outer wall of the sealing door. A magnetic sealing ring is connected to the inner wall of the sealing door. An ECU core controller is installed on the rear side of the inner cavity of the frame box body. A power supply is installed on the bottom of the inner cavity of the frame box body. An AC interface is provided at the end of the power supply. A ventilation and heat dissipation port is opened on the left end face of the frame box body. A touch screen, a power switch, a status light, a USB interface, and a network cable interface are installed on the right end face of the frame box body.
[0006] Preferably, in a cell genetic experiment incubator, a temperature control pipe is built into the chamber wall of the culture chamber. A culture dish is installed at the bottom of the inner cavity of the culture chamber. A sample delivery pipe and a water mist spray pipe are inserted into the inner side wall of the culture chamber. A multi-signal acquisition board is connected to the inner side wall of the culture chamber. A vacuum extraction pipe and a gas delivery pipe communicate with the top wall of the culture chamber. An electron microscope lens, a lighting lamp, and an ultraviolet lamp are connected to the top of the inner cavity of the culture chamber. Solenoid valves are installed in the temperature control pipe, the sample delivery pipe, the water mist spray pipe, the vacuum extraction pipe, and the gas delivery pipe. A waste liquid recovery pipe is inserted into the inner side wall of the culture chamber. The culture dish is equipped with ceramic balls. A rubber pipe communicates between the waste liquid recovery pipe and the ceramic balls. A micro pump is installed in the waste liquid recovery pipe. A plurality of the waste liquid recovery pipes communicate with a waste discharge pipe in common. The end of the waste liquid recovery pipe extends out of the frame box body.
[0007] Preferably, in a cell genetic experiment incubator, a temperature sensor, a humidity sensor, a carbon dioxide concentration sensor, and a pressure sensor are built into the multi-signal acquisition board. A stepping motor and a manual adjustment knob are connected to the upper end of the electron microscope lens. The end of the manual adjustment knob extends out of the front surface of the frame box body.
[0008] Preferably, in a cell genetic experiment incubator, a vacuum pump is installed at the bottom of the frame box body. An air extraction pipe communicates with the input end of the vacuum pump. The end of the vacuum extraction pipe communicates with the air extraction pipe. A fixed bracket is connected to the left side wall of the frame box body. A carbon dioxide compression gas cylinder is detachably installed in the fixed bracket. An air pump is installed at the rear end of the frame box body. An air supply pipe communicates with the output end of the air pump. The end of the gas delivery pipe communicates with the air supply pipe. Two groups of air inlet pipes communicate with the input end of the air pump. One group of the air inlet pipes communicates with the carbon dioxide compression gas cylinder. The other group of the air inlet pipes extends out of the frame box body. Solenoid valves are installed in both groups of the air inlet pipes.
[0009] Preferably, in a cell genetic experiment incubator, a distilled water tank is installed in the distilled water filling port. A piezoelectric ceramic wafer is installed at the bottom of the distilled water tank. A fan is embedded in the rear side wall of the distilled water tank. A humidifying air duct communicates with the front side wall of the distilled water tank. The end of the water mist spray pipe communicates with the humidifying air duct.
[0010] Preferably, in a cell genetic experiment incubator, a sample collection pipe is inserted into the sample collection port. A sampling port cover is installed at the upper end of the sample collection pipe. The end of the sample delivery pipe communicates with the sample collection pipe.
[0011] Preferably, in a cell genetic experiment incubator, a main water inlet pipe is connected to the left inner wall of the frame box body, a main water outlet pipe is connected to the right inner wall of the frame box body, a water pump is installed in the main water inlet pipe, a variable-temperature water supply device is communicated between the main water inlet pipe and the main water outlet pipe, both ends of the temperature control pipe are respectively communicated with the main water inlet pipe and the main water outlet pipe, and a communication check valve is installed on one side of the temperature control pipe close to the main water outlet pipe.
[0012] Preferably, in a cell genetic experiment incubator, there are six culture chambers arranged in an array, the culture chambers are made of aluminum alloy material, the inner wall surface of the culture chambers is covered with a graphene coating, and an XPS extruded insulation board is provided between adjacent culture chambers.
[0013] The beneficial effects of the present invention are as follows:
[0014] 1. The structure of the present invention is reasonably designed. There are multiple independent culture chambers in the frame box body, which can separate and independently culture the culture samples. Before culturing, ultraviolet lamps are used for sterilization treatment. By setting different culture environment parameters for comparison and reference, it is convenient to explore the best culture environment plan.
[0015] 2. The present invention has only one culture sample placement port, and the whole culture process is automated, reducing external environmental interference and the risk of secondary infection. During the culture process, waste liquid treatment can be carried out without opening the sealed door to prevent external environmental pollution.
[0016] 3. The present invention can observe the culture samples in real time, understand the progress of cell culture without taking out the culture samples, avoid culture interruption, and reduce the risk of culture failure.
[0017] 4. The present invention can monitor and adjust the culture environment parameters in real time to ensure that the environment parameters are stably at the set value and ensure the cell culture effect.
[0018] 5. The present invention can store the culture data and generate a data report, which is convenient for enriching more database content and providing more targeted control data for subsequent similar experiments. BRIEF DESCRIPTION OF THE DRAWINGS
[0019] In order to more clearly illustrate the technical solutions of the present invention, the drawings required for use in the description of the specific embodiments will be briefly introduced below. Obviously, the drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.
[0020] Figure 1 Schematic diagram of the overall structure of the present invention Figure 1 ;
[0021] Figure 2 Schematic diagram of the overall structure of the present invention Figure 2 ;
[0022] Figure 3 Schematic diagram of the internal structure of the culture chamber in the present invention;
[0023] Figure 4 Schematic diagram of the structure of the multi-signal acquisition board in the present invention;
[0024] Figure 5 Schematic diagram of the structure of the vacuum pump in the present invention;
[0025] Figure 6 Block diagram of the working principle of the air pump in the present invention;
[0026] Figure 7 Cross-sectional structure diagram of the distillation water tank in the present invention;
[0027] Figure 8 Cross-sectional structure diagram of the sample collection tube in the present invention;
[0028] Figure 9 Block diagram of the working principle of the temperature control tube in the present invention;
[0029] Figure 10 Schematic diagram of the structure of the temperature control tube in the present invention;
[0030] Figure 11 Schematic diagram of the structure of the waste discharge pipe in the present invention.
[0031] In the figure: 1. Frame box body; 2. Base; 3. Distilled water filling port; 4. Sample collection port; 5. Culture chamber; 6. Sealed door; 7. Identification plate; 8. Door handle; 9. Magnetic sealing ring; 10. ECU core controller; 11. Power supply; 12. AC interface; 13. Ventilation and heat dissipation port; 14. Touch screen; 15. Power switch; 16. Status light; 17. USB interface; 18. Network cable interface; 101. Vacuum pump; 102. Exhaust pipe; 103. Fixed bracket; 104. Carbon dioxide compressed gas cylinder; 105. Air pump; 106. Air supply pipe; 107. Induction pipe; 301. Distilled water tank; 302. Piezoelectric ceramic wafer; 303. Fan; 304. Humidification air duct; 401. Sample collection tube; 402. Sampling port cover; 501. Temperature control pipe; 502. Culture dish; 503. Sample delivery pipe; 504. Water mist spraying pipe; 505. Multi-signal acquisition board; 506. Vacuum extraction pipe; 507. Gas delivery pipe; 508. Electron microscope lens; 509. Lighting lamp; 510. Ultraviolet lamp; 511. Waste liquid recovery pipe; 512. Ceramic ball; 513. Rubber pipe; 514. Micro pump; 515. Waste discharge pipe; 551. Temperature sensor; 552. Humidity sensor; 553. Carbon dioxide concentration sensor; 554. Air pressure sensor; 581. Stepper motor; 582. Manual adjustment knob; 111. Main water inlet pipe; 112. Main water outlet pipe; 113. Water pump; 114. Variable temperature water supply device; 115. Connecting check valve. Detailed implementation manners
[0032] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.
[0033] Embodiment 1
[0034] Please refer to Figures 1-10As shown in the figure, this embodiment is a cell genetic experiment incubator, which includes a frame box body 1. The four corners of the bottom of the frame box body 1 are connected with pedestals 2. A distilled water filling port 3 and a sample collection port 4 are opened at the top of the frame box body 1. A plurality of culture chambers 5 are arranged on the front side of the inner cavity of the frame box body 1. A sealing door 6 is hinged at the front end of the culture chamber 5. A signboard 7 and a door handle 8 are connected to the outer wall of the sealing door 6. A magnetic sealing ring 9 is connected to the inner wall of the sealing door 6. An ECU core controller 10 is installed at the rear side of the inner cavity of the frame box body 1. A power supply 11 is installed at the bottom of the inner cavity of the frame box body 1. An AC interface 12 is provided at the end of the power supply 11. A ventilation and heat dissipation port 13 is opened on the left end face of the frame box body 1. A touch screen 14, a power switch 15, a status light 16, a USB interface 17 and a network cable interface 18 are installed on the right end face of the frame box body 1.
[0035] A temperature control pipe 501 is built into the chamber wall of the culture chamber 5. A culture dish 502 is installed at the bottom of the inner cavity of the culture chamber 5. A sample delivery pipe 503 and a water mist injection pipe 504 are inserted into the inner side wall of the culture chamber 5. A multi-signal acquisition board 505 is connected to the inner side wall of the culture chamber 5. A vacuum extraction pipe 506 and a gas delivery pipe 507 are communicated with the top wall of the culture chamber 5. An electron microscope lens 508, a lighting lamp 509 and an ultraviolet lamp 510 are connected to the top of the inner cavity of the culture chamber 5. Solenoid valves are installed in the temperature control pipe 501, the sample delivery pipe 503, the water mist injection pipe 504, the vacuum extraction pipe 506 and the gas delivery pipe 507. Different variable supplies can be respectively connected through multiple pipelines. A waste liquid recovery pipe 511 is inserted into the inner side wall of the culture chamber 5. The culture dish 502 is configured with ceramic balls 512. A rubber pipe 513 is communicated between the waste liquid recovery pipe 511 and the ceramic balls 512. A micro pump 514 is built into the waste liquid recovery pipe 511. A plurality of waste liquid recovery pipes 511 are jointly communicated with a waste discharge pipe 515. The end of the waste liquid recovery pipe 511 extends out of the frame box body 1.
[0036] A temperature sensor 551, a humidity sensor 552, a carbon dioxide concentration sensor 553 and a pressure sensor 554 are built into the multi-signal acquisition board 505, which is convenient for real-time detection of internal environmental parameters. A stepping motor 581 and a manual adjustment knob 582 are connected to the upper end of the electron microscope lens 508. The end of the manual adjustment knob 582 extends out of the front end face of the frame box body 1, which is convenient for adjusting the lens picture.
[0037] Six culture chambers 5 are provided and arranged in an array, which is convenient for setting multiple culture environments for control culture. The culture chamber 5 is made of aluminum alloy material. Considering the stable structure, the inner wall surface of the culture chamber 5 is covered with a graphene coating to improve the heat preservation effect inside the chamber. An XPS extruded insulation board is provided between adjacent culture chambers 5, which can isolate the temperature.
[0038] The specific implementation manner of the present invention is as follows:
[0039] Six culture chambers 5 are arranged inside the frame box body 1 of this device. Each culture chamber 5 is relatively independent, and different culture environments can be set for each culture chamber 5. After turning on the power switch 15, they start running respectively. The touch screen 14 is lit up, and the status light 16 indicates blue (the system is normal and ready to run). The touch screen 14 displays two parts of content. One part is the real-time monitoring video of each culture chamber 5, and the other part is the operating status of each subsystem inside the device. Before the culture experiment, the culture environment is sterilized by the ultraviolet lamp 510. On the touch screen 14, select the initial settings for the control experiment, that is, set the temperature, humidity, carbon dioxide gas concentration, and culture time of each sample culture chamber 5 respectively. It also supports setting culture conditions by stage. The touch screen 14 sends the set parameters to the ECU core controller 10 through the communication line, and uses the ECU core controller 10 to control the operation of each electrical component. By setting a temperature sensor 551, a humidity sensor 552, a carbon dioxide concentration sensor 553, and a pressure sensor 554 in the multi-signal acquisition board 505, the internal environmental parameters of the culture chamber 5 can be monitored in real time. By using multiple pipelines to connect different supply units of variables, the culture environment can be adjusted to ensure the cell culture effect;
[0040] By connecting an external storage device through the USB interface 17, the culture data can be recorded and stored, and a data report of the whole culture process will be automatically generated after the culture is completed. By connecting to an external network through the network cable interface 18, it is convenient to compare the culture data with the cloud database, automatically record the change values and time of each stage. When the set time is reached or the sample is infected, the status light 16 turns red for warning, reminding the operator to analyze and process in time. After the culture is completed, just open the sealed door 6 and take out the culture container. During the culture process, regularly start the corresponding micro pump 514 of the culture chamber, which can introduce the waste liquid in the culture dish 502 along the ceramic ball 512 into the rubber tube 513, and then discharge it out of the frame box body 1 along the path of the waste liquid recovery pipe 511 - waste discharge pipe 515.
[0041] Embodiment 2
[0042] On the basis of Embodiment 1, a vacuum pump 101 is installed at the bottom of the frame box body 1. The input end of the vacuum pump 101 is connected with an air extraction pipe 102. The end of the vacuum extraction pipe 506 is connected with the air extraction pipe 102. The left side wall of the frame box body 1 is connected with a fixed bracket 103. A carbon dioxide compression gas cylinder 104 is detachably installed in the fixed bracket 103. An air pump 105 is installed at the rear end of the frame box body 1. The output end of the air pump 105 is connected with an air supply pipe 106. The end of the gas delivery pipe 507 is connected with the air supply pipe 106. The input end of the air pump 105 is connected with two groups of air intake pipes 107. One group of air intake pipes 107 is connected with the carbon dioxide compression gas cylinder 104, and the other group of air intake pipes 107 extends out of the frame box body 1. Solenoid valves are installed in both groups of air intake pipes 107 to facilitate the adjustment of the gas environment.
[0043] Inside the distilled water filling port 3, there is a distilled water tank 301 installed. At the bottom of the distilled water tank 301, there is a piezoelectric ceramic wafer 302 installed. On the rear side wall of the distilled water tank 301, there is a fan 303 embedded. On the front side wall of the distilled water tank 301, there is a humidifying air duct 304 connected. The end of the water mist spraying pipe 504 is connected to the humidifying air duct 304, which is convenient for adjusting the humidity environment.
[0044] Inside the sample collection port 4, there is a sample collection tube 401 inserted. At the upper end of the sample collection tube 401, there is a sampling port cover 402 installed. The end of the sample delivery tube 503 is connected to the sample collection tube 401, which is convenient for sample splitting.
[0045] On the left side of the inner wall of the frame box body 1, there is a main water inlet pipe 111 connected. On the right side of the inner wall of the frame box body 1, there is a main water outlet pipe 112 connected. Inside the main water inlet pipe 111, there is a water pump 113 installed. Between the main water inlet pipe 111 and the main water outlet pipe 112, there is a variable temperature water supply device 114 connected. Both ends of the temperature control pipe 501 are respectively connected to the main water inlet pipe 111 and the main water outlet pipe 112. On the side of the temperature control pipe 501 close to the main water outlet pipe 112, there is a connected check valve 115 installed, which is convenient for ensuring the temperature environment of the culture chamber 5.
[0046] The specific implementation manner of the present invention is as follows:
[0047] In this embodiment, before the culture test, the culture environment is sterilized by the ultraviolet lamp 510. On the touch screen 14, the initial settings of the control test are selected, that is, the temperature, humidity, CO 2 gas concentration, and culture time of each sample culture in the culture chamber 5 are respectively set; it also supports setting multiple stages, and the temperature, humidity, CO 2 gas concentration, and culture time of each stage can be set differently;
[0048] The touch screen 14 sends the set parameters to the ECU core controller 10 through the communication line. Using the ECU core controller 10, first disconnect all the solenoid valves leading to the culture chamber 5, so that the culture chamber 5 forms a closed space. After the air extraction pipe 102 is unblocked, control the vacuum pump 101 to work, and the air in the culture chamber 5 can be evacuated. According to the data fed back by the air pressure sensor 554, the culture chamber 5 is adjusted to the preset negative pressure value; once the preset negative pressure value is reached, disconnect the solenoid valve at the exhaust port of the culture chamber 5 that meets the negative pressure requirement until all the culture chambers 5 reach the preset negative pressure value, and then disconnect all the solenoid valves at the exhaust ports of the culture chambers 5;
[0049] The ECU core controller 10 is used to start an oscillation circuit with an oscillation frequency of about 20KHz, and the output is connected to the piezoelectric ceramic chip 302 placed at the bottom of the distilled water tank 301, thereby converting electrical energy into mechanical vibration with ultrasonic frequency, namely, the diaphragm. The diaphragm acts on the water surface to increase the energy of water molecules and escapes from the water surface to form water mist, which is blown out by the fan 303 and enters the water mist injection pipe 504 from the humidification air duct 304, and then flows into each small culture chamber 5 of the incubator. According to the feedback data of the humidity sensor 552, the corresponding small culture chambers 5 in the incubator are adjusted to the set air humidity value. After the air humidity feedback from the humidity sensor 552 reaches the set value, the solenoid valve of the corresponding small culture chamber 5 channel is closed.
[0050] The temperature control tubes 501 distributed throughout the inner wall of the culture chamber 5 can evenly and stably control the temperature in the culture chamber 5, and because the graphene coating is attached to the inner wall surface, the temperature in the room can be quickly kept stable and uniform. When the room temperature of a small culture chamber 5 of the incubator deviates from the set value, the variable temperature water supply device 114 is used in conjunction with the water pump 113 to provide hot water for exchange, so that the temperature in the corresponding small culture chamber 5 reaches the set temperature, and the corresponding water pump 113 is turned off to keep the temperature consistent, so as to maintain the temperature in each small culture chamber 5 of the incubator consistent with the set temperature;
[0051] The culture sample is placed in the sample collection port 4. After the ECU core controller 10 confirms that the temperature of the culture chamber 5 has reached the set value, it controls the solenoid valve on the sample delivery tube 503 to open. Since each culture chamber 5 is currently in a negative pressure state, the culture sample will be sucked from the sample delivery tube 503 into the culture dish 502, thereby performing cell culture;
[0052] After the culture sample is in place, the ECU core controller 10 can control and adjust the carbon dioxide concentration, that is, start the air pump 105, open the corresponding solenoid valve to allow carbon dioxide or air to enter the gas delivery pipe 507, and then flow into each small culture chamber 5 of the incubator. When the carbon dioxide concentration of a small culture chamber 5 of the incubator reaches the set value, the carbon dioxide concentration feedback from the carbon dioxide concentration detection sensor 553 reaches the set value, and then the air pump 105 and the solenoid valve corresponding to the small culture chamber 5 in the incubator are closed;
[0053] During the sample culture process, each sensor feeds back the real-time environmental parameters in the culture chamber 5 in real time. When the real-time environmental parameters deviate from the set values, the corresponding system is started synchronously to maintain the stability of the culture environment. The conventional parameters that can be set during the culture process are temperature 37°C, CO 2 The concentration was 5%, the pH value was 7.2-7.4, and the relative saturation humidity was 95%. The single parameter was adjusted by comparing the environment, so as to conduct sample monitoring control;
[0054] Turn on the illumination lamp 509 and the electron microscope lens 508. The lens images are transmitted to the touch screen 14 synchronously. Use the stepper motor 581 to adjust the lens for autofocus, which can make the lens images clearer and facilitate subsequent processing and analysis. You can also use the manual adjustment knob 582 to adjust it until the shooting position is accurate.
[0055] In the description of this specification, the description with reference to terms such as "one embodiment", "example", "specific example", etc. means that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in a suitable manner in any one or more embodiments or examples.
[0056] The preferred embodiments of the present invention disclosed above are only used to help explain the present invention. The preferred embodiments do not describe all the details in detail, nor do they limit the invention to the specific embodiments described. Obviously, many modifications and variations can be made according to the content of this specification. These embodiments are selected and specifically described in this specification to better explain the principles and practical applications of the present invention, so that those skilled in the art can understand and utilize the present invention well. The present invention is only limited by the claims and their full scope and equivalents.
Claims
1. A cell genetics experiment incubator, comprising a frame box (1), characterized in that: The four corners of the bottom of the frame box (1) are connected to a base (2); the top of the frame box (1) is provided with a distilled water inlet (3) and a sample collection port (4); the front side of the inner cavity of the frame box (1) is provided with a plurality of culture chambers (5); the front end of the culture chamber (5) is hinged with a sealed door (6); the outer wall of the sealed door (6) is connected with a signboard (7) and a door handle (8); the inner wall of the sealed door (6) is connected with a magnetic sealing ring (9); the rear side of the inner cavity of the frame box (1) is provided with an ECU core controller (10); the bottom of the inner cavity of the frame box (1) is provided with a power supply (11); the end of the power supply (11) is provided with an AC interface (12); the left end face of the frame box (1) is provided with a ventilation and heat dissipation port (13); the right end face of the frame box (1) is provided with a touch screen (14), a power switch (15), a status light (16), a USB interface (17) and a network cable interface (18).
2. A cell genetics experiment incubator according to claim 1, characterized in that: The wall of the culture chamber (5) is provided with a temperature control tube (501), the bottom of the inner cavity of the culture chamber (5) is provided with a culture dish (502), the inner wall of the culture chamber (5) is plugged with a sample delivery tube (503) and a water mist injection tube (504), the inner wall of the culture chamber (5) is connected with a multi-signal acquisition board (505), the top wall of the culture chamber (5) is connected with a vacuum extraction tube (506) and a gas delivery tube (507), the top of the inner cavity of the culture chamber (5) is connected with an electron microscope lens (508), an illuminating lamp (509) and an ultraviolet lamp (510), the temperature control tube (501), the sample delivery tube (503) and the water mist injection tube (504) are connected with the inner wall of the culture chamber (5), and the vacuum extraction tube (506) and the gas delivery tube (507) are connected with the top of the inner cavity of the culture chamber (5). Solenoid valves are installed in the delivery pipe (503), the water mist injection pipe (504), the vacuum extraction pipe (506) and the gas delivery pipe (507); a waste liquid recovery pipe (511) is inserted into the inner wall of the culture chamber (5); the culture dish (502) is provided with a ceramic ball (512); a rubber tube (513) is connected between the waste liquid recovery pipe (511) and the ceramic ball (512); a micro pump (514) is built into the waste liquid recovery pipe (511); a plurality of waste liquid recovery pipes (511) are commonly connected to a waste discharge pipe (515); and an end of the waste liquid recovery pipe (511) extends out of the frame box (1).
3. A cell genetics experiment incubator according to claim 2, characterized in that: The multi-signal acquisition board (505) is equipped with a temperature sensor (551), a humidity sensor (552), a carbon dioxide concentration sensor (553) and an air pressure sensor (554); the upper end of the electron microscope lens (508) is connected to a stepping motor (581) and a manual adjustment knob (582); the end of the manual adjustment knob (582) extends out of the front end surface of the frame box (1).
4. A cell genetic experiment incubator according to claim 2, characterized in that: A vacuum pump (101) is installed at the bottom of the frame box (1), the input end of the vacuum pump (101) is connected to an exhaust pipe (102), the end of the vacuum extraction pipe (506) is connected to the exhaust pipe (102), the left side wall of the frame box (1) is connected to a fixed bracket (103), a carbon dioxide compressed gas cylinder (104) is detachably installed in the fixed bracket (103), and the rear end of the frame box (1) is installed with an air pump (101). 05), the output end of the air pump (105) is connected to the air supply pipe (106), the end of the gas delivery pipe (507) is connected to the air supply pipe (106), the input end of the air pump (105) is connected to two groups of air bleed pipes (107), one group of the air bleed pipes (107) is connected to the carbon dioxide compressed gas cylinder (104), and the other group of the air bleed pipes (107) extends out of the frame box (1), and solenoid valves are installed in the two groups of the air bleed pipes (107).
5. A cell genetics experiment incubator according to claim 2, characterized in that: A distilled water tank (301) is installed in the distilled water inlet (3), a piezoelectric ceramic chip (302) is installed at the bottom of the distilled water tank (301), a fan (303) is embedded in the rear side wall of the distilled water tank (301), a humidification air duct (304) is connected to the front side wall of the distilled water tank (301), and the end of the water mist injection pipe (504) is connected to the humidification air duct (304).
6. A cell genetics experiment incubator according to claim 2, characterized in that: A sample collection tube (401) is inserted into the sample collection port (4), a sampling port cover (402) is installed at the upper end of the sample collection tube (401), and the end of the sample delivery tube (503) is connected to the sample collection tube (401).
7. A cell genetics experiment incubator according to claim 2, characterized in that: The left side of the inner wall of the frame box (1) is connected to a main water inlet pipe (111), and the right side of the inner wall of the frame box (1) is connected to a main water outlet pipe (112); a water pump (113) is installed in the main water inlet pipe (111); a variable temperature water supply device (114) is connected between the main water inlet pipe (111) and the main water outlet pipe (112); two ends of the temperature control pipe (501) are respectively connected to the main water inlet pipe (111) and the main water outlet pipe (112); and a connecting check valve (115) is installed on the side of the temperature control pipe (501) close to the main water outlet pipe (112).
8. The cell genetic experiment incubator according to claim 1, characterized in that: The culture chambers (5) are provided with six and arranged in an array; the culture chambers (5) are made of an aluminum alloy material; the inner wall surface of the culture chamber (5) is covered with a graphene coating; and XPS extruded insulation boards are provided between adjacent culture chambers (5).