Shaking table device for biological cell culture

Abstract

The invention relates to the technical field of cell culture, and discloses a shaking table device for biological cell culture, the shaking table device comprises a shaking table host and a loading assembly, the loading assembly comprises a constant temperature plate mounted at the top of the shaking table host, the top of the constant temperature plate is fixedly connected with a first toothed bar, and the outer wall of the first toothed bar is slidably connected with a supporting plate; when a conical block rotates, a second sliding rod is extruded, so that a first rubber ring and a second rubber ring are expanded and attached to the outer wall of the culture dish, and the joint of the culture dish upper cover and the culture dish can be sealed through attachment of the first rubber ring and the second rubber ring; according to the culture dish, external bacteria can be prevented from entering the culture dish through a gap between the culture dish upper cover and the culture dish, a sterile environment in the cell culture process is ensured, meanwhile, the culture dish can be firmly mounted in the supporting plate through expansion of a second rubber ring and a first rubber ring, and the phenomenon that the culture dish is damaged when the supporting plate swings is avoided. The problem of collision caused by loose installation of the culture dish is solved, and the safety of the equipment in use is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of cell culture, and specifically relates to a shaker device for biological cell culture. Background Art

[0002] In the field of biological cell culture, an accurate and stable culture environment plays a crucial role in the growth, reproduction of cells and the accuracy of experimental research. Traditional cell culture shaker devices have gradually revealed many defects in practical applications and are difficult to meet the increasingly stringent scientific research and production requirements.

[0003] There are potential hazards in the fixing method of the culture dish in the shaker. Simply clamping or relying solely on the placement groove to fix the culture dish is extremely likely to cause the culture dish to loosen during the long-term and high-intensity shaking of the shaker. The displacement and collision of the culture dish will not only damage the cell structure being cultured, but may also cause the culture dish to break, resulting in the leakage of the culture solution and affecting the subsequent experimental process; Since it is difficult to achieve complete sealing between the upper cover and the dish body of the culture dish, pollutants such as bacteria and microorganisms in the external air are extremely likely to invade. The pollution of external bacteria may lead to cell lesions and death, interfere with the authenticity and reliability of experimental data, and hinder scientific research progress and the output of results; In order to ensure the uniform distribution of cells in the culture solution and full contact with nutrients, traditional shakers often cannot effectively solve the problem of cells adhering to the inner wall of the culture dish simply by relying on the overall shaking action. Some cells are prone to adhere to the culture dish wall, forming local aggregates, which are difficult to mix fully with the culture solution, resulting in uneven cell growth environment, abnormal morphological differentiation, etc., affecting the experimental results.

[0004] Therefore, a shaker device for biological cell culture is proposed. Summary of the Invention

[0005] The purpose of the present invention is to provide a shaker device for biological cell culture to solve the problems raised in the above background art.

[0006] To achieve the above purpose, the present invention provides the following technical solution: A shaker device for biological cell culture, including a shaker main body and a loading component. The loading component includes a thermostatic plate installed on the top of the shaker main body. A first toothed rod is fixedly connected to the top of the thermostatic plate. A tray is slidably connected to the outer wall of the first toothed rod. Feeding holes are evenly formed through the tray. The interior of the pallet is evenly provided with a sealing component for fixing the culture dish. The sealing component includes annular sealing cabins symmetrically and fixedly connected to the top of the pallet in a linear array distribution. One side of the annular sealing cabin close to the bottom of the upper cover of the culture dish is fixedly communicated with a first rubber ring, and one side of the annular sealing cabin close to the side wall of the culture dish is fixedly communicated with a second rubber ring. The bottom of the annular sealing cabin is slidably connected with second sliding rods distributed in an annular array, and third springs are fixedly connected between the top of the inner cavity of the annular sealing cabin and the top of the second sliding rods.

[0007] Preferably, a toothed ring is rotatably connected below the annular sealing cabin inside the pallet. Conical blocks are fixedly connected to the inner wall of the toothed ring in an annular array distribution, and baffles are fixedly connected to the tops of the conical blocks.

[0008] Preferably, a closed space is formed inside the second sliding rod due to the blockage of the second sliding rod. The second rubber ring is attached to the side wall of the culture dish, and the first rubber ring is attached to the bottom of the upper cover of the culture dish. The side of the outer wall of the conical block away from the baffle is an inclined surface, and the bottom of the inclined surface of the conical block is located below the second sliding rod.

[0009] Preferably, a transmission component is symmetrically arranged above the pallet. The transmission component includes toothed shafts symmetrically rotatably connected to the top of the pallet. An irregular toothed plate is slidably connected inside the pallet. The top of the irregular toothed plate meshes with the toothed shafts, and the side wall of the toothed shaft meshes with the toothed ring.

[0010] Preferably, a knocking component is arranged at the bottom of the pallet. The knocking component includes a sliding ring rotatably connected to the bottom of the pallet. A counterweight is fixedly connected to the bottom of the sliding ring. One side of the sliding ring away from the counterweight is rotatably connected to a knocking rod. A ball head is fixedly connected to the top of the knocking rod. Corrugated grooves adapted to the feeding holes are evenly formed at the bottom of the pallet, and the ball head at the top of the knocking rod is slidably connected inside the corrugated grooves.

[0011] Preferably, a moving component is arranged at the bottom of the pallet. The moving component includes a folding plate fixedly connected to the bottom of the pallet. First sliding rods are symmetrically and fixedly connected to the bottom of the pallet. A cross plate is slidably connected between the two first sliding rods. A first spring is fixedly connected between the bottom of the cross plate and the bottom of the first sliding rods. Convex blocks are symmetrically and fixedly connected to the top of the pallet. Through grooves adapted to the convex blocks are symmetrically formed in the cross plate. Slide frames are symmetrically slidably connected inside the through grooves. Second springs are fixedly connected between the opposite sides of the slide frames and the inner wall of the cross plate. A second toothed rod is fixedly connected to the top of the thermostat plate. The pallet is slidably connected to the outer wall of the second toothed rod, and the adjacent sides of the slide frames are clamped to the outer wall of the second toothed rod.

[0012] Preferably, the horizontal plate is located above the folding plate. The side of the bump away from the second toothed rod is an inclined surface. When the second spring is not compressed, the carriage is clamped to the outer wall of the second toothed rod. When the first spring is not stretched, the horizontal plate is located below the bump.

[0013] Preferably, the toothed shafts are all meshed with the outer wall of the first toothed rod.

[0014] Compared with the prior art, the beneficial effects of the present invention are as follows: 1. Due to the extrusion of the second sliding rod generated when the conical block rotates, the first rubber ring and the second rubber ring expand and fit onto the outer wall of the culture dish, enabling the connection between the upper cover of the culture dish and the culture dish to be sealed through the fitting of the first rubber ring and the second rubber ring. This can prevent external bacteria from entering the culture dish through the gap between the upper cover of the culture dish and the culture dish, ensuring a sterile environment during the cell culture process. At the same time, due to the expansion of the second rubber ring and the first rubber ring, the culture dish can be firmly installed inside the tray, avoiding the problem of collision caused by the loose installation of the culture dish when the tray swings, and improving the safety of the equipment during use; 2. By knocking the side wall of the culture dish with the knocking rod, the vibration of the culture dish shell can be generated, causing the cells attached to the inner wall of the culture dish to fall off and float in the culture solution, enabling the surface of the cells to come into complete contact with the culture solution, avoiding the problem that the cells cannot come into contact with the culture solution locally when attached to the inner wall of the culture dish, and enabling the cells to be efficiently cultivated in the culture dish. Description of the Drawings

[0015] Figure 1 It is a three-dimensional schematic diagram of the overall structure of the present invention; Figure 2 It is a side view schematic diagram of the overall structure of the present invention; Figure 3 It is a partial schematic diagram of the structure of the moving component of the present invention; Figure 4 It is a sectional view schematic diagram of the structure of the moving component of the present invention; Figure 5 It is a sectional view schematic diagram of the structure of the closing component of the present invention; Figure 6 For the present invention Figure 5 The enlarged schematic diagram of the structure at A in; Figure 7 It is an exploded schematic diagram of the structure of the closing component of the present invention; Figure 8 It is a sectional view schematic diagram of the internal structure of the tray of the present invention; Figure 9 It is an exploded schematic diagram of the structure of the knocking component of the present invention.

[0016] In the figure: 1. Shaker main body; 2. Loading component; 3. Moving component; 4. Closing component; 5. Transmission component; 6. Knocking component; 21. Constant temperature plate; 22. First toothed rod; 23. Support plate; 31. Folding plate; 32. First sliding rod; 33. Horizontal plate; 34. First spring; 35. Protrusion; 36. Chute; 37. Slide carriage; 38. Second spring; 39. Second toothed rod; 41. Annular seal chamber; 42. First rubber ring; 43. Second rubber ring; 44. Third spring; 45. Second sliding rod; 46. Toothed ring; 47. Tapered block; 48. Baffle; 51. Toothed shaft; 52. Special-shaped toothed plate; 61. Slip ring; 62. Counterweight; 63. Knocking rod; 64. Ball head; 65. Corrugated groove. Detailed implementation manners

[0017] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0018] Embodiments of the present invention Please refer to Figures 1 to 9 , a shaker device for biological cell culture, including a shaker main body 1 and a loading assembly 2. The loading assembly 2 includes a constant temperature plate 21 installed on the top of the shaker main body 1. A first toothed rod 22 is fixedly connected to the top of the constant temperature plate 21. A support plate 23 is slidably connected to the outer wall of the first toothed rod 22. Feeding holes are uniformly formed through the support plate 23; A sealing assembly 4 for fixing the culture dish is uniformly arranged inside the support plate 23. The sealing assembly 4 includes annular seal chambers 41 symmetrically and fixedly connected to the top of the support plate 23 in a linear array distribution. A first rubber ring 42 is fixedly communicated with the side of the annular seal chamber 41 close to the bottom of the upper cover of the culture dish. A second rubber ring 43 is fixedly communicated with the side of the annular seal chamber 41 close to the side wall of the culture dish. Second sliding rods 45 are slidably connected to the bottom of the annular seal chamber 41 in an annular array distribution. Third springs 44 are fixedly connected between the top of the inner cavity of the annular seal chamber 41 and the top of the second sliding rods 45.

[0019] A toothed ring 46 is rotatably connected inside the support plate 23 and below the annular seal chamber 41. Tapered blocks 47 are fixedly connected to the inner wall of the toothed ring 46 in an annular array distribution. Baffles 48 are fixedly connected to the tops of the tapered blocks 47.

[0020] The inner cavity of the second sliding rod 45 is blocked by the second sliding rod 45 to form a closed space. The second rubber ring 43 is attached to the side wall of the culture dish, and the first rubber ring 42 is attached to the bottom of the upper cover of the culture dish. The side of the outer wall of the tapered block 47 away from the baffle 48 is an inclined surface, and the bottom of the inclined surface of the tapered block 47 is located below the second sliding rod 45.

[0021] Above the support plate 23, a transmission assembly 5 is symmetrically arranged. The transmission assembly 5 includes tooth shafts 51 symmetrically and rotatably connected to the top of the support plate 23. An irregular tooth plate 52 is slidably connected inside the support plate 23. The top of the irregular tooth plate 52 meshes with the tooth shafts 51, and the side wall of the tooth shaft 51 meshes with the tooth ring 46.

[0022] The tooth shafts 51 are all meshed with the outer wall of the first tooth rod 22.

[0023] At the bottom of the support plate 23, a knocking assembly 6 is provided. The knocking assembly 6 includes a sliding ring 61 rotatably connected to the bottom of the support plate 23. A counterweight 62 is fixedly connected to the bottom of the sliding ring 61. A knocking rod 63 is rotatably connected to the side of the sliding ring 61 away from the counterweight 62. A ball head 64 is fixedly connected to the top of the knocking rod 63. Corrugated grooves 65 adapted to the feeding holes are uniformly opened at the bottom of the support plate 23. The ball head 64 at the top of the knocking rod 63 is slidably connected inside the corrugated grooves 65.

[0024] At the bottom of the support plate 23, a moving assembly 3 is provided. The moving assembly 3 includes a folding plate 31 fixedly connected to the bottom of the support plate 23. First sliding rods 32 are symmetrically and fixedly connected to the bottom of the support plate 23. A cross plate 33 is slidably connected between the two first sliding rods 32. A first spring 34 is fixedly connected between the bottom of the cross plate 33 and the bottom of the first sliding rods 32. Convex blocks 35 are symmetrically and fixedly connected to the top of the support plate 23. Through grooves 36 adapted to the convex blocks 35 are symmetrically opened on the cross plate 33. Slide frames 37 are symmetrically and slidably connected inside the through grooves 36. Second springs 38 are fixedly connected between the opposite sides of the slide frames 37 and the inner wall of the cross plate 33. A second tooth rod 39 is fixedly connected to the top of the constant temperature plate 21. The support plate 23 is slidably connected to the outer wall of the second tooth rod 39. The adjacent sides of the slide frames 37 are clamped to the outer wall of the second tooth rod 39.

[0025] The cross plate 33 is located above the folding plate 31. The side of the convex block 35 away from the second tooth rod 39 is an inclined surface. When the second spring 38 is not compressed, the slide frames 37 are clamped to the outer wall of the second tooth rod 39. When the first spring 34 is not stretched, the cross plate 33 is located below the convex block 35.

[0026] Working principle: When the cells in the culture dish need to be cultured by the equipment, the staff puts the culture dishes into the feeding holes on the support plate 23 one by one. The cup body of the culture dish will slide down in the feeding hole. When the bottom of the upper cover of the culture dish abuts against the annular sealing chamber 41, the culture dish is limited and cannot continue to slide down inside the support plate 23; When all the culture dishes are put into the delivery hole, the staff needs to make the support plate 23 slide downward on the first gear rod 22. At this time, the staff will push the folding plate 31 upward. After the folding plate 31 is bent, it will squeeze the cross plate 33 upward. When the cross plate 33 slides upward on the outer wall of the first slide rod 32, the first spring 34 will be stretched and elastically extended. When the cross plate 33 moves upward, it will drive the slide 37 inside it to move upward together. After the slide 37 moves to contact the protrusion 35, the inclined surface of the protrusion 35 will squeeze the slide 37. The squeezed slide 37 will slide relatively away inside the cross plate 33 and make the second spring 38 contract. After the slide 37 slides, it will no longer be engaged with the outer wall of the second gear rod 39. At this time, the support plate 23 is no longer limited by the engagement of the slide 37 and the second gear rod 39 and can slide on the outer wall of the first gear rod 22. After the staff adjusts the position of the support plate 23 until the bottom of the culture dish contacts the top of the constant temperature plate 21, the staff no longer moves the folding plate 31. The folding plate 31 rebounds after being free from force, and the slide 37 resumes the engaged state with the second gear rod 39, thereby positioning the support plate 23 on the first gear rod 22 and stabilizing the contact between the culture dish and the constant temperature plate 21.

[0027] When the support plate 23 moves downward, the gear shaft 51 will be driven to rotate. When the gear shaft 51 rotates, it will drive the special-shaped toothed plate 52 to slide horizontally inside the support plate 23 through meshing. When the special-shaped toothed plate 52 slides inside the support plate 23, it will drive multiple toothed rings 46 to rotate. During the rotation of the toothed ring 46, the multiple toothed rings 46 and the conical block 47 on the inner wall will rotate together. During the rotation of the conical block 47, the inclined surface of the conical block 47 will contact the bottom of the second sliding rod 45, and as the conical block 47 rotates, it will cause the second sliding rod 45 to form an upward extrusion. After being extruded, the second sliding rod 45 slides toward the inside of the annular sealed cabin 41. After the internal cavity of the annular sealed cabin 41 is extruded, the first rubber ring 42 and the second rubber ring 43 will be inflated and expanded. After the first rubber ring 42 is expanded, it will fit with the bottom of the culture dish cover, and after the second rubber ring 43 is expanded, it will fit with the side wall of the culture dish body, so that the first rubber ring 42 and the second rubber ring 43 respectively seal the culture dish body and the culture dish cover.

[0028] The conical block 47 squeezes the second slide bar 45 when it rotates, so that the first rubber ring 42 and the second rubber ring 43 expand and fit onto the outer wall of the culture dish, so that the connection between the culture dish cover and the culture dish can be sealed by the fit of the first rubber ring 42 and the second rubber ring 43, which can prevent foreign bacteria from entering the culture dish through the gap between the culture dish cover and the culture dish, ensuring a sterile environment during the cell culture process. At the same time, the expansion of the second rubber ring 43 and the first rubber ring 42 can securely install the culture dish inside the support plate 23, avoiding collision problems caused by loose installation of the culture dish when the support plate 23 swings, thereby improving the safety of the equipment when in use.

[0029] After the culture dish is installed on the support plate 23, the staff starts the shaker main body 1. The shaker main body 1 drives the constant temperature plate 21 to start shaking. During the shaking of the support plate 23, due to the setting of the counterweight 62, the slip ring 61 at the bottom of the support plate 23 will generate a centrifugal force during the shaking of the support plate 23 and drive the slip ring 61 to rotate at the bottom of the support plate 23. During the rotation of the slip ring 61 at the bottom of the support plate 23, the ball head 64 at the top of the knocking rod 63 will slide inside the corrugated groove 65. Affected by the shape of the corrugated groove 65, the knocking rod 63 will reciprocally swing inside the slip ring 61 during the sliding of the ball head 64 inside the corrugated groove 65, so that the bottom of the knocking rod 63 is constantly in a state of knocking on the side wall of the culture dish. By knocking on the side wall of the culture dish with the knocking rod 63, the culture dish shell can be vibrated, so that the cells attached to the inner wall of the culture dish fall off and float in the culture solution, enabling the cell surface to be in complete contact with the culture solution, avoiding the problem that the cells cannot come into contact with the culture solution locally when attached to the inner wall of the culture dish, and enabling the cells to be efficiently cultivated in the culture dish.

[0030] It should be noted that in this article, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variant thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements not only includes those elements, but also includes other elements not expressly listed, or also includes elements inherent to such process, method, article or device. Without further limitation, an element defined by the statement "including a..." does not exclude the existence of additional identical elements in the process, method, article or device including the said element.

[0031] Although the embodiments of the present invention have been shown and described, it will be understood by those of ordinary skill in the art that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A shaking table device for biological cell culture, comprising a shaking table main unit (1) and a loading assembly (2), characterized in that: The loading assembly (2) comprises a constant temperature plate (21) mounted on the top of the shaking table main unit (1), the top of the constant temperature plate (21) is fixedly connected to a first gear rod (22), the outer wall of the first gear rod (22) is slidably connected to a support plate (23), and the support plate (23) is evenly penetrated with delivery holes; The interior of the support plate (23) is evenly provided with a sealing component (4) for fixing the culture dish. The sealing component (4) includes an annular sealing cabin (41) symmetrically fixedly connected to the top of the support plate (23) in a linear array arrangement. A first rubber ring (42) is fixedly connected to the side of the annular sealing cabin (41) close to the bottom of the culture dish upper cover. A second rubber ring (43) is fixedly connected to the side of the annular sealing cabin (41) close to the side wall of the culture dish. A second sliding rod (45) is slidably connected to the bottom of the annular sealing cabin (41) in an annular array arrangement. A third spring (44) is fixedly connected between the top of the inner cavity of the annular sealing cabin (41) and the top of the second sliding rod (45).

2. A shaking table device for biological cell culture according to claim 1, characterized in that: A gear ring (46) is rotatably connected inside the support plate (23) and below the annular sealed cabin (41). Conical blocks (47) are fixedly connected to the inner wall of the gear ring (46) in an annular array. Baffles (48) are fixedly connected to the tops of the conical blocks (47).

3. A shaking table device for biological cell culture according to claim 2, characterized in that: The inner cavity of the second sliding rod (45) is blocked by the second sliding rod (45) to form a closed space, the second rubber ring (43) is in contact with the side wall of the culture dish, the first rubber ring (42) is in contact with the bottom of the upper cover of the culture dish, and the outer wall of the conical block (47) is formed into an inclined surface on the side away from the baffle (48), and the bottom of the inclined surface of the conical block (47) is located below the second sliding rod (45).

4. A shaking table device for biological cell culture according to claim 1, characterized in that: A transmission assembly (5) is symmetrically arranged above the support plate (23), the transmission assembly (5) comprising a gear shaft (51) symmetrically rotatably connected to the top of the support plate (23), a special-shaped gear plate (52) is slidably connected inside the support plate (23), the top of the special-shaped gear plate (52) is meshed with the gear shaft (51), and the side wall of the gear shaft (51) is meshed with the gear ring (46).

5. A shaking table device for biological cell culture according to claim 1, characterized in that: A knocking assembly (6) is provided at the bottom of the support plate (23), and the knocking assembly (6) comprises a slip ring (61) rotatably connected to the bottom of the support plate (23), a counterweight (62) is fixedly connected to the bottom of the slip ring (61), a knocking rod (63) is rotatably connected to the side of the slip ring (61) away from the counterweight (62), a ball head (64) is fixedly connected to the top of the knocking rod (63), and corrugated grooves (65) adapted to the delivery holes are evenly formed at the bottom of the support plate (23), and the ball head (64) at the top of the knocking rod (63) is slidably connected to the inside of the corrugated grooves (65).

6. A shaking table device for biological cell culture according to claim 1, characterized in that: A moving assembly (3) is provided at the bottom of the support plate (23), and the moving assembly (3) includes a folding plate (31) fixedly connected to the bottom of the support plate (23); a first sliding rod (32) is symmetrically fixedly connected to the bottom of the support plate (23); a cross plate (33) is slidably connected between two of the first sliding rods (32); a first spring (34) is fixedly connected between the bottom of the cross plate (33) and the bottom of the first sliding rod (32); a protrusion (35) is symmetrically fixedly connected to the top of the support plate (23); The plate (33) is symmetrically provided with a slide groove (36) adapted to the protrusion (35), the slide groove (36) is symmetrically slidably connected to a slide rack (37) inside, a second spring (38) is fixedly connected between the side of the slide rack (37) away from the inner wall of the cross plate (33), a second gear rod (39) is fixedly connected to the top of the constant temperature plate (21), the support plate (23) is slidably connected to the outer wall of the second gear rod (39), and the side of the slide rack (37) close to the outer wall of the second gear rod (39) is clamped.

7. A shaking table device for biological cell culture according to claim 6, characterized in that: The transverse plate (33) is located above the folding plate (31); a side of the protrusion (35) away from the second gear rod (39) is an inclined surface; when the second spring (38) is not squeezed, the slide (37) is engaged with the outer wall of the second gear rod (39); when the first spring (34) is not stretched, the transverse plate (33) is located below the protrusion (35).

8. A shaking table device for biological cell culture according to claim 4, characterized in that: The gear shafts (51) are all meshed with the outer wall of the first gear rod (22).

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