New ganoderma lucidum strain Guizhi No.9 and application thereof
By developing the new red zhi strain Guizhi No. 9, the problem of insufficient trace elements of existing red zhizhi was solved, and the characteristics of natural selenium-rich and high-speed rail were realized, which improved its health performance and commercial value.
Patent Information
- Application Number
- CN202510207218.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-25
- Publication Date
- 2025-06-13
AI Technical Summary
In the prior art, the trace elements content of Chizhi is less studied, especially the natural content of selenium is low, and the iron content of Chizhi is generally low, making it difficult to meet the needs of selenium-rich and high-speed rail health products.
A new red zhi strain, Guizhi No. 9, was developed. This strain was bred and domesticated in the wild, has the characteristics of naturally selenium-rich and high iron-containing properties, and has a high ability to secrete cellulase and hemicellulase.
The high selenium and high iron content of Guizhi No. 9 Chizhi strain significantly improves its health care performance, can more effectively utilize the cellulose in the culture medium, provide better germplasm resources, and enhance the commercial value of Chizhi.
Smart Images
Figure CN120137792A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the field of biotechnology, and particularly to a new Ganoderma lucidum strain Guizhi 9 and its applications.
Background Art
[0002] Ganoderma lucidum is the fruiting body of the fungus Ganoderma lucidum in the family Polyporaceae. Due to its extremely high medicinal value, it is commercially cultivated on a large scale. Ganoderma lucidum is usually divided into two different species, Ganoderma lucidum (red) and Ganoderma sinense (purple). In ancient times, Ganoderma lucidum was considered to have the effects of resurrection and prolonging life. Traditional Chinese medicine uses it for restlessness of mind, loss of appetite, insomnia with many dreams, cough and asthma due to deficiency of lung qi, etc. Modern research shows that Ganoderma lucidum plays a huge role in diseases such as liver injury [2], cancer, cardiovascular diseases, brain injury, oxidative damage, hyperglycemia, inflammation, immunity, and aging, and has very important research value and market prospects. Currently, Ganoderma lucidum has been widely used in food and medicine. The main chemical components of Ganoderma lucidum are polysaccharides, triterpenoids, sterols, amino acids, alkaloids, nucleosides, fatty acids, and trace elements, etc. Among them, polysaccharides and triterpenoids are its main active components and are also the most studied components.
[0003] However, in the prior art, the research on the trace element content of Ganoderma lucidum is less. In the prior art, we also found that Ganoderma lucidum fruiting bodies basically do not naturally contain selenium. If we want to produce selenium-rich Ganoderma lucidum varieties, we need to add selenium elements to the culture medium additionally. If there are varieties that naturally contain selenium, it will greatly improve the health care ability of Ganoderma lucidum. The iron content in Ganoderma sinense is generally higher than that in Ganoderma lucidum. The iron content in Ganoderma sinense can reach more than 90 μg / g, while few Ganoderma lucidum varieties can reach more than 90 μg / g. In addition, in the prior art, Ganoderma lucidum belongs to wood-growing edible fungi and has a strong ability to secrete cellulase, which helps to improve the decomposition of cellulose in the substrate. If these enzymes can be extracted and applied, it will greatly expand the application of woody culture media in the prior art. Therefore, in order to strengthen the utilization of Ganoderma lucidum germplasm resources and develop more excellent germplasm resources, it is necessary to continuously optimize and cultivate Ganoderma lucidum germplasm resources and screen out Ganoderma lucidum varieties with higher natural selenium and iron content and better commerciality.
Summary of the Invention
[0004] In view of the above, the object of the present invention is to provide a new Ganoderma lucidum strain: Guizhi 9. This variety was selected and domesticated from the wild by the applicant. Compared with other existing Ganoderma lucidum varieties, this variety has a unique appearance. In addition, compared with other varieties, it has a higher selenium content and the ability to produce cellulase and hemicellulase. Its health care performance and the utilization ability of the culture medium are better than other varieties, and it can provide more excellent germplasm resources for Ganoderma lucidum.
[0005] To achieve the above object, the present invention screened out a Ganoderma lingzhi strain Guizhi No. 9, with the preservation number of CGMCC NO. 41339. It belongs to edible fungi and the Ganoderma lingzhi species. The said Ganoderma is preserved in the General Microbiological Center of the China Committee for Culture Collection of Microorganisms, address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, with the preservation number of CGMCC NO. 41339 and the preservation date of June 14, 2024.
[0006] The present invention also includes the application of the Ganoderma lingzhi strain Guizhi No. 9 as a parent in cross-breeding.
[0007] The present invention also includes the spores obtained from the Ganoderma lingzhi strain Guizhi No. 9.
[0008] The present invention also includes the mycelium obtained from the Ganoderma lingzhi strain Guizhi No. 9.
[0009] The present invention also includes the fruiting body obtained from the Ganoderma lingzhi strain Guizhi No. 9.
[0010] The present invention also includes the application of the fruiting body of the Ganoderma lingzhi strain Guizhi No. 9 in food processing.
[0011] The present invention also includes the application of the fruiting body of the Ganoderma lingzhi strain Guizhi No. 9 in the preparation of selenium-rich and / or iron-rich health products.
[0012] The present invention also includes the application of the mycelium of the Ganoderma lingzhi strain Guizhi No. 9 in the extraction of cellulase and / or hemicellulase.
[0013] The present invention has the following beneficial effects:
[0014] 1. The Ganoderma lingzhi strain Guizhi No. 9 of this application was screened and domesticated by the research group from the wild environment. The results of various tests show that: there are significant differences in mycelial growth and fruiting body appearance between this strain and existing similar varieties. The edge of the mushroom cap is round and blunt, with a relatively deep groove. Moreover, through the determination of inclusions, it is also found that: this strain is a natural selenium-rich Ganoderma lingzhi strain, and its iron content is also higher than that of other Ganoderma lingzhi varieties. In addition, this strain also has a high ability to secrete cellulase and hemicellulase, and can effectively utilize the cellulose in the nutrient bag. It is a rare Ganoderma lingzhi variety rich in health care selenium and high in iron. This variety can greatly enhance the commercial value of Ganoderma lingzhi, and at the same time, it can also provide richer germplasm resources for Ganoderma lingzhi.
Description of the Drawings
[0015] Figure 1 It is the antagonistic experiment result of Guizhi No. 9 and similar varieties. Among them, A is the comparison between Guizhi No. 9 and Hunong Ganoderma lingzhi No. 1. In the figure: Guizhi No. 9 is on the left and Hunong Ganoderma lingzhi No. 1 is on the right; B is the comparison between Guizhi No. 9 and Guizhi No. 9 and Chuanzhi No. 6: Guizhi No. 9 is on the left and Chuanzhi No. 6 is on the right.
[0016] Figure 2 It is the comparison diagram of the mycelial bundle thickness between Guizhi No. 9 strain and similar varieties.
[0017] Figure 3 It is the comparison diagram of the colony density and morphology between Guizhi No. 9 strain and similar varieties.
[0018] Figure 4 It is the comparison diagram of the colony pigment between Guizhi No. 9 strain and similar varieties.
[0019] Figure 5 It is the upper surface diagram of the fruiting body mushroom cap of Guizhi No. 9 strain and similar varieties.
[0020] Figure 6 It is the lower surface diagram of the fruiting body mushroom cap of Guizhi No. 9 strain and similar varieties.
[0021] Figure 7 It is the sectional view of the fruiting body of Guizhi No. 9 strain and similar varieties.
[0022] Figure 8 It is the microscope diagram of the basidiospores of Guizhi No. 9 strain and similar varieties.
Detailed Embodiments
[0023] The present invention will be further described below in conjunction with the drawings, examples and tests.
[0024] Example 1:
[0025] In this example, a strain of Ganoderma lingzhi, Guizhi No. 9, was screened out. Its preservation number is CGMCC NO. 41339. It belongs to edible fungi and the Ganoderma lingzhi species. The said Ganoderma is preserved in the General Microbiological Center of the China General Microbiological Culture Collection Center, address: No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing. The preservation number is CGMCC NO. 41339, and the preservation date is June 14, 2024.
[0026] The above-mentioned Ganoderma lingzhi strain Guizhi No. 9 was isolated, domesticated and cultivated by the applicant from wild Ganoderma lingzhi in Tianlin County, Baise City, Guangxi. The antagonism map of this strain with other varieties of Ganoderma lingzhi can be seen in Figure 1 , where Figure 1 Figure A in it is the antagonism map of Guizhi No. 9 (left) and Hunong Ganoderma No. 1 (right); Figure B is the antagonism map of Guizhi No. 9 (left) and Hunong Ganoderma No. 1 (right). From the antagonism map, it can be seen that there is an obvious antagonistic line between Guizhi No. 9 and similar varieties, and it can be determined as different varieties.
[0027] The fruiting body morphology of Guizhi No. 9 can be seen in Figures 5 - 7 : where Figure 7 is the upper surface view of the fruiting body, Figure 6 is the lower surface view of the fruiting body, Figure 7 is the cross-sectional comparison view of the fruiting body; it can be seen from the figure that the formation time of the fruiting body cap is extremely early, the stipe length is shorter, the stipe shape is cylindrical, the cap thickness is thick, the cap size is medium, the cap transverse diameter / stipe length is large, the cap thickness / cap transverse diameter is large, the cap is kidney-shaped, the color of the upper surface of the fruiting body is light reddish-brown, the color of the lower surface is yellow, there are radial longitudinal ridges on the upper surface of the cap, the annulus on the upper surface of the cap is medium, the edge of the cap is round and blunt, the flesh of the fruiting body is obviously layered, the color of the flesh is light brown, the texture of the stipe is hard, and the texture of the cap is hard.
[0028] From Figure 5 and Figure 6 's comparison map, it can be seen that the morphology of Guizhi No. 9 is significantly different from similar varieties: Hunong Ganoderma No. 1 and Chuanzhi No. 6. There is only an obvious circular pattern around the periphery of its cap, no circular pattern inside, and the cap shape is obviously kidney-shaped. The color of the cap is grayish, and the back color is closer to Chuanzhi No. 6 from the lower surface of the cap.
[0029] Example 2:
[0030] This example is for morphological identification of the Ganoderma lingzhi strain Guizhi No. 9 screened out in Example 1. Fruiting body trait comparison and identification test:
[0031] Cultivation experiment for fruiting body production: (1) Preparation of mother spawn: Transfer the PDA slant culture to sawdust medium and incubate at 25 °C for later use. (2) Preparation of fungus bags: Fill each bag with 1 / 2 - 2 / 3 volume of miscellaneous wood medium, sterilize at 0.14 MPa - 0.15 MPa (temperature 126 °C - 127 °C) for 2.5 h, and cool for later use. (3) Inoculation: Transfer the mother spawn to the fungus bags, inoculate 10 bags for each number. After the mycelium fills the bag for 3 - 7 days, transfer to the culture rack for ventilation management. The temperature during the mycelium growth stage is set at 18 °C - 32 °C, and the temperature during the fruiting body production stage is set at 25 °C - 28 °C. Manage the entire production process routinely. The duration of the entire cultivation experiment is 5 months.
[0032] Comparison of agronomic traits of fruiting bodies: Conducted in accordance with the standards of the Agricultural Industry Standard of the People's Republic of China "Guidelines for the Testing of Specificity, Consistency and Stability of New Plant Varieties - Ganoderma lucidum". All data are calculated and presented as averages. For specific traits, see Table 1:
[0033] Table 1 Comparative chart of morphological characteristics between Guizhi No. 9 and the similar variety Wuzhi No. 2
[0034]
[0035]
[0036] From the results recorded in Table 1, the mycelial cords of the new variety Guizhi No. 9 are not as thick as those of the similar varieties Hunong Ganoderma lucidum No. 1 and Chuanzhi No. 6, and the colony morphology is relatively fluffier than that of the similar varieties.
[0037] For the comparison diagram of mycelia between Guizhi No. 8 and the similar variety, see Figures 2 - 4 , for the morphological characteristics of the fruiting body, see Figures 5 - 7 , and the microscope image of basidiospores is as shown in Figure 8 . As can be seen from the figure: Among the thickness of the mycelial cords of Guizhi No. 9, the mycelial density is high, the colony morphology is slightly fluffy, the colony has pigment, the mycelial growth rate is slow, the primordium formation time is earlier, the fruiting body pileus formation time is extremely early, the stipe length is shorter, the stipe shape is cylindrical, the pileus thickness is thick, the pileus size is medium, the pileus transverse diameter / stipe length is large, the pileus thickness / pileus transverse diameter is large, the pileus is kidney-shaped, the upper surface color of the fruiting body is light reddish-brown, the lower surface color is yellow, there are radial longitudinal ridges on the upper surface of the pileus, the annulus on the upper surface of the pileus is medium, the pileus edge is round and blunt, the context is obviously stratified, the context color is light brown, the stipe texture is hard, the pileus texture is hard, the spores are oval with a large protrusion at one end, and the spore length / width is large.
[0038] By Figure 5 and Figure 6From the comparison chart, it can be seen that the morphology of Guizhi No. 9 is significantly different from that of the similar varieties: Hunong Ganoderma lucidum No. 1 and Chuanzhi No. 6. Its pileus only has an obvious annular pattern around the edge, without an annular pattern inside, and the pileus shape is obviously kidney-shaped. The color of the pileus is grayish, and from the lower surface of the pileus, the back color is closer to Chuanzhi No. 6.
[0039] Example 3:
[0040] Analysis of the differences in the internal substances between the new strain Guizhi No. 9 and the similar variety: Wuzhi No. 2
[0041] 1. Nutritional components:
[0042] Under the same conditions, Guizhi No. 9 and the similar variety: Wuzhi No. 2 were cultivated to produce mushrooms. After fruiting, the fruiting bodies of the same batch with the same maturity were collected. According to the relevant detection standards for nutritional components, the protein, polysaccharide, and total amino acid contents of the Ganoderma lucidum fruiting bodies were detected. The specific analysis results are shown in Table 2:
[0043] Table 2 Comparison of nutritional components between Guizhi No. 9 and its parental strains
[0044]
[0045]
[0046] Note: The same lowercase letter superscript or no superscript for data in the same row indicates no significant difference (P>0.05), and different lowercase letter superscripts indicate significant difference (P<0.05).
[0047] It can be seen from Table 2 that through detection and analysis, the selenium content, iron content, and polysaccharide content of the new strain Guizhi No. 9 are significantly higher than those of the similar varieties Hunong Ganoderma lucidum No. 1 and Chuanzhi No. 6 (P<0.05), while the total flavonoid and total phenol contents have no significant difference compared with the similar varieties. The triterpene content is higher than that of Hunong Ganoderma lucidum No. 1 and lower than that of Chuanzhi No. 6, and there is no significant advantage compared with the similar varieties.
[0048] 2. Extracellular enzyme activity:
[0049] The applicant also measured the extracellular enzyme activity of the mycelium of the new strain Guizhi No. 9 and the similar variety, as follows:
[0050] (1) Preparation of crude enzyme solution After the Ganoderma lucidum mycelium filled the cultivation bags, 20 cultivation bags were taken from each generation of the strain. The upper 5 cm of the substrate was taken, freeze-dried, and then ground at low temperature through a 20-mesh sieve. After mixing evenly, an appropriate amount of the substrate powder was taken, and distilled water or extraction solution was added according to the mass-volume ratio of 1:10. The supernatant obtained after soaking in a constant temperature water bath and centrifugation was the crude enzyme solution.
[0051] (2) Enzyme activity assay: The ABTS method was used to determine the laccase activity. The amount of enzyme required to oxidize 1 μmol of the substrate ABTS per minute per milligram of protein was defined as one enzyme activity unit. For the determination of cellulase, hemicellulase, and filter paper enzyme activities, the 3,5-dinitrosalicylic acid (DNS) colorimetric method was used. The amount of enzyme required to catalyze the production of 1 mg of glucose per minute per milligram of protein was defined as one enzyme activity unit respectively.
[0052] Table 3 Results of extracellular enzyme activity determination of mycelia
[0053]
[0054] Note: The same lowercase letter superscript or no superscript for data in the same row indicates no significant difference (P > 0.05), and different lowercase letter superscripts indicate significant difference (P < 0.05).
[0055] As can be seen from Table 3, the hemicellulase and cellulase activities of Guizhi 9 are greater than those of similar varieties, indicating that the new strain Guizhi 9 of this application has a better ability to decompose cellulose.
[0056] Example 4:
[0057] The above Ganoderma lucidum strain Guizhi 9 can be used as a parent to hybridize with other Ganoderma lucidum strains of other parents to obtain Ganoderma lucidum germplasm resources with richer colors and more special morphologies.
[0058] In summary, the Ganoderma lucidum strain Guizhi 9 of this application was screened and domesticated by the applicant from the wild environment. After verification, it was found that: among the thickness of the mycelial bundles of this strain, the mycelial density is dense, the colony morphology is slightly fluffy, the colony has pigments, the mycelial growth rate is slow, the primordium formation time is earlier, the fruiting body pileus formation time is extremely early, the stipe length is shorter, the stipe shape is cylindrical, the pileus thickness is thick, the pileus size is medium, the pileus transverse diameter / stipe length is large, the pileus thickness / pileus transverse diameter is large, the pileus is kidney-shaped, the upper surface color of the fruiting body is light reddish-brown, the lower surface color is yellow, there are radial longitudinal ridges on the upper surface of the pileus, the annulus on the upper surface of the pileus is medium, the pileus edge is round and blunt, the context is obviously stratified, the context color is light brown, the stipe texture is hard, the pileus texture is hard, the spores are oval with a large protrusion at one end, the spore length / width is large. In terms of the contained substances: the selenium and iron contents in the fruiting body are higher than those of the existing Ganoderma lucidum varieties. In terms of enzyme activity: the hemicellulase and cellulase activities of the above strain are both greater than those of the existing Ganoderma lucidum varieties, indicating that the new strain Guizhi 9 of this application has a better ability to decompose cellulose and is a Ganoderma lucidum strain with excellent performance.
[0059] The above description is a detailed description of the preferred and feasible embodiments of the present invention, but the embodiments are not used to limit the patent application scope of the present invention. Any equivalent changes or modifications made under the technical spirit disclosed by the present invention should fall within the patent scope covered by the present invention.
Claims
1. Ganoderma lingzhi strain Guizhi No. 9, with the preservation number of CGMCC NO.41339.
2. Use of the Ganoderma lingzhi strain Guizhi No. 9 as claimed in claim 1 as a parent in hybrid breeding.
3. Spores obtained by culturing the Ganoderma lingzhi strain Guizhi No. 9 as claimed in claim 1.
4. Mycelium obtained by culturing the Ganoderma lingzhi strain Guizhi No. 9 as claimed in claim 1.
5. A fruiting body obtained by culturing the Ganoderma lingzhi strain Guizhi No. 9 as claimed in claim 1.
6. Use of the fruiting body according to claim 5 in food processing.
7. Use of the fruiting body according to claim 5 in preparing selenium-rich and / or high-iron health products.
8. Use of the mycelium according to claim 4 in extracting cellulase and / or hemicellulase.