Cordyceps sobolifera spore powder and production method thereof

Abstract

The invention provides cordyceps sobolifera spore powder and a production method thereof, and the production method comprises the following steps: S1, mixing whole wheat flour and soybean meal to form a material, and adding water into the material to form a culture medium; s2, preparing the culture medium into a spherical culture medium in a pelleting machine, and sterilizing the spherical culture medium; s3, inoculating cordyceps sobolifera strains into the sterilized spherical culture medium, and culturing; and S4, after culture is finished, collecting to obtain cordyceps sobolifera spore powder. The method improves the yield of the cordyceps sobolifera spore powder, is suitable for industrialized production of the cordyceps sobolifera spore powder, and has the advantages of short period, high economic benefit and wide application value.

Description

Technical Field

[0001] The present invention relates to the field of food processing in agricultural technology, and particularly to a cordyceps cicadae spore powder and a production method thereof. Background Art

[0002] Cordyceps cicadae is a fungus of the genus Cordyceps, which parasitizes in the nymphs of cicadas and forms a "complex of insect and fungus". Spores (conidia) are the reproductive units of Cordyceps cicadae, rich in active ingredients, and are commonly used for improving eyesight, calming the nerves, and anti-convulsion. Traditional Chinese medicine believes that it is cold in nature and belongs to the liver meridian, and is used to treat infantile convulsions, insomnia, red and swollen eyes, etc.

[0003] Modern scientific research has found active ingredients such as polysaccharides, nucleosides, cordycepin, cyclic peptides, ergosterol, etc. in Cordyceps cicadae. It can be used to activate macrophages and NK cells; scavenge free radicals (verified by DPPH / ABTS experiments); induce apoptosis of tumor cells (such as liver cancer HepG2 cells); improve the cognitive function of Alzheimer's disease model mice (by inhibiting Aβ deposition). Although Cordyceps cicadae spores have been produced by artificial cultivation methods, the existing production methods have problems such as a long production cycle, being not suitable for industrial production, and low economic benefits. Summary of the Invention

[0004] In view of this, the purpose of the present invention is to provide a cordyceps cicadae spore powder and a production method thereof to improve the above problems.

[0005] An embodiment of the present invention provides a production method of cordyceps cicadae spore powder, which includes the following steps:

[0006] S1, mixing whole wheat flour and soybean meal powder to form a material, and adding water to the material to form a culture medium;

[0007] S2, making the culture medium into spherical culture medium in a pill making machine, and sterilizing the spherical culture medium; wherein, the diameter of the spherical culture medium is less than or equal to 1 cm;

[0008] S3, inoculating Cordyceps cicadae strain into the sterilized spherical culture medium and culturing;

[0009] S4, after the culturing is completed, collecting the cordyceps cicadae spore powder.

[0010] Preferably, in step S1, by weight, in the material: 60 - 70% of whole wheat flour, 30 - 40% of soybean meal powder; the ratio of the material to water is 1:0.8 - 1.

[0011] Preferably, in step S2, the diameter of the spherical culture medium is less than or equal to 1 cm.

[0012] Preferably, step S2 specifically includes:

[0013] Use a pill-making machine to make the prepared culture medium into spherical culture media with a diameter of 0.6 - 1 cm; among them, to prevent adhesion on the outside, a thin layer of wheat bran powder is adhered to the outside of the spherical culture medium.

[0014] Place the spherical culture media in a box in a single-layer manner for sterilization.

[0015] Preferably, in step S3, the inoculation amount of Cordyceps cicadae strain is 5 - 10%.

[0016] Preferably, in step S3, the culture process is as follows:

[0017] Cultivate for 7 - 8 days in the dark at a temperature of 22 - 24°C;

[0018] After the Cordyceps cicadae mycelium grows on the surface of the ball, carry out light cultivation; the light cultivation intensity is 300 - 500 Lux, the temperature is 25 - 26°C, the humidity is controlled at 60 - 70%, the light time is 12 - 16 h / d, and the cultivation time is 20 - 25 days.

[0019] Preferably, the spore powder yield of Cordyceps cicadae spore powder reaches 18 - 25%; among them, the spore powder yield = dry weight of spore powder / (dry weight of spherical culture medium + dry weight of spore powder) × 100%.

[0020] The embodiment of the present invention also provides a Cordyceps cicadae spore powder, which is produced by the production method as described above.

[0021] In the production method of Cordyceps cicadae spore powder provided by the present invention, soybean meal (with a protein content of 40 - 48%) is added to the materials, which significantly improves the protein content of the culture medium. The present invention makes the culture medium into a spherical shape, and in the same plane, its growth plane is increased by 3 times. At the same time, it is found that spherical shapes with a diameter of 1 cm and below basically do not grow fruiting bodies, and all nutrients are used for growing spore powder, which greatly increases the yield of spore powder, and the spore powder yield can reach 18 - 25%.

[0022] In summary, the present invention improves the yield of Cordyceps cicadae spore powder, is suitable for industrial production of Cordyceps cicadae spore powder, has a short cycle, high economic benefits, and has broad application value. Detailed Embodiments

[0023] Next, in combination with the embodiments of the present invention, the technical solutions of the present invention will be clearly and completely described. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0024] Example 1

[0025] The formula of the spherical culture medium is as follows: by weight, 60% whole wheat flour, 40% soybean meal powder, and the ratio of material to water is 1:0.8. Use a pill-making machine to make the prepared culture medium into balls with a diameter of 0.6 cm. To prevent adhesion on the outside, a thin layer of wheat bran powder is adhered to the outside.

[0026] Put the spherical culture medium into plastic boxes, only one layer is placed, and 100 g (dry weight) of spherical culture medium is placed in each box, with a total of 20 boxes. Sterilize at 121 °C for 30 minutes, inoculate after cooling. The cicada flower liquid species is prepared by a conventional method, and the inoculation amount is 5%.

[0027] Cultivate in the dark at a temperature of 22 °C for 8 days, and then expose to light after the cicada flower cordyceps mycelium grows on the surface of the balls. The light intensity for light cultivation is 300 Lux, the temperature is 25 °C, the humidity is controlled at 60%, the light time is 12 h / d, and the cultivation time is 25 days.

[0028] After all the spores are mature, harvest and dry them. After weighing, the biological conversion rate of the spore powder reaches the yield. Dry the 20 boxes of balls with grown spore powder, and sieve out the spore powder through a 100-mesh closed sieve to obtain 1287 g of spherical culture medium and 326 g of spore powder. The spore powder yield is 20.2%. The spore powder yield = spore powder dry weight / (spherical culture medium dry weight + spore powder dry weight) × 100%.

[0029] In this example, the feeding amount is 2000 grams. The reduction in harvest is due to the relatively high moisture content of the whole wheat flour and soybean meal powder and the consumption of a part during the growth stage.

[0030] Example 2

[0031] The formula of the spherical culture medium: 65% whole wheat flour, 35% soybean meal powder, and the ratio of material to water is 1:0.9. Use a pill-making machine to make the prepared culture medium into balls with a diameter of 0.8 cm. To prevent adhesion on the outside, a thin layer of wheat bran powder is adhered to the outside.

[0032] Put the spherical culture medium into plastic boxes, only one layer is placed, and 100 g (dry weight) of small balls is placed in each box, with a total of 20 boxes. Sterilize at 121 °C for 30 minutes, inoculate after cooling. The cicada flower liquid species is prepared by a conventional method, and the inoculation amount is 8%.

[0033] Cultivate in the dark at a temperature of 24 °C for 7 days, and then expose to light after the cicada flower cordyceps mycelium grows on the surface of the balls. The light intensity for light cultivation is 500 Lux, the temperature is 25 °C, the humidity is controlled at 60%, the light time is 14 h / d, and the cultivation time is 23 days.

[0034] After all the isospores are mature, harvest them. Dry the 20 boxes of spore powder balls that have grown well. Sieve out the spore powder through a 100-mesh closed sieve to obtain 1208 g of small ball culture medium, 399 g of spore powder, and the spore powder yield is 24.8%.

[0035] In this example, the feeding amount is 2000 grams. The reduction in harvest is due to the relatively high moisture content of wheat wholemeal flour and soybean meal powder and the consumption of a part during the growth stage.

[0036] Example 3

[0037] Spherical culture medium formula: 70% wheat wholemeal flour, 30% soybean meal powder, the ratio of material to water is 1:1. Use a pill-making machine to make the prepared culture medium into balls with a diameter of 1 cm. To prevent adhesion on the outside, apply a thin layer of wheat bran powder on the outside.

[0038] Put the spherical culture medium into plastic boxes, only place one layer, put 100 g of small balls in each box (calculated by dry weight), and a total of 20 boxes are placed. Sterilize at 121 °C for 30 minutes, inoculate after cooling. The cicada flower liquid seed is prepared by a conventional method, and the inoculation amount is 10%.

[0039] Cultivate in the dark at a temperature of 24 °C for 7 days. After the cicada flower cordyceps mycelium grows on the surface of the balls, carry out light cultivation. The light cultivation intensity is 500 Lux, the temperature is 26 °C, the humidity is controlled at 60%, the light time is 16 h / d, and the cultivation time is 20 days.

[0040] After all the isospores are mature, harvest them. Dry the 20 boxes of spore powder balls that have grown well. Sieve out the spore powder through a 100-mesh closed sieve to obtain 1312 g of spherical culture medium, 298 g of spore powder, and the spore powder yield is 18.5%.

[0041] Example 4

[0042] Spherical culture medium formula: 65% wheat wholemeal flour, 35% soybean meal powder, the ratio of material to water is 1:0.9. Use a pill-making machine to make the prepared culture medium into balls with a diameter of 1.2 cm. To prevent adhesion on the outside, apply a thin layer of wheat bran powder on the outside.

[0043] Put the spherical culture medium into plastic boxes, only place one layer, put 100 g of small balls in each box (calculated by dry weight), and a total of 20 boxes are placed. Sterilize at 121 °C for 30 minutes, inoculate after cooling. The cicada flower liquid seed is prepared by a conventional method, and the inoculation amount is 10%.

[0044] Cultivate in the dark at a temperature of 24 °C for 7 days. After the cicada flower cordyceps mycelium grows on the surface of the balls, carry out light cultivation. The light cultivation intensity is 500 Lux, the temperature is 26 °C, the humidity is controlled at 60%, the light time is 14 h / d, and the cultivation time is 20 days.

[0045] After all the isospores are mature, harvest them. Dry the 20 boxes of spore powder balls that have grown well. Sieve out the spore powder through a 100-mesh closed sieve to obtain 1477 g of spherical medium and 168 g of spore powder, with a spore powder yield of 10.2%.

[0046] In this embodiment, it can be seen that after the diameter of the spherical medium is greater than 1 cm, the spore powder yield decreases significantly. This is mainly because part of the medium with a diameter above 1 cm is used for the growth of fruiting bodies, thereby affecting the spore powder yield.

[0047] In summary, in the production method of Cordyceps cicadae spore powder provided by the present invention, soybean meal (protein content 40 - 48%) is added to the materials, significantly increasing the protein content of the medium. The present invention makes the medium spherical, increasing its growth plane by 3 times in the same plane. At the same time, it is found that spherical shapes with a diameter of 1 cm and below basically do not grow fruiting bodies, and all nutrients are used for growing spore powder, which greatly increases the output of spore powder, and the spore powder yield can reach 18 - 25%.

[0048] The present invention increases the output of Cordyceps cicadae spore powder, is suitable for industrial production of Cordyceps cicadae spore powder, has a short cycle, high economic benefits, and broad application value.

[0049] The above is only the preferred embodiment of the present invention, and the protection scope of the present invention is not limited to the above embodiments. All technical solutions within the idea of the present invention belong to the protection scope of the present invention.

Claims

1. A method for producing spore powder of Cordyceps sinensis, characterized in that: The steps include: S1, mixing whole wheat flour and soybean meal powder to form a material, and adding water to the material to form a culture medium; S2, making the culture medium into a spherical culture medium in a pellet making machine, and sterilizing the spherical culture medium; S3, inoculating Cordyceps sinensis strains into the sterilized spherical culture medium for culturing; S4, after the cultivation is completed, the spore powder of Cordyceps sinensis is collected.

2. The method for producing spore powder of Cordyceps sinensis according to claim 1, characterized in that: In step S1, the material comprises, by weight: 60-70% whole wheat flour and 30-40% soybean meal powder; and the ratio of the material to water is 1:0.8-1.

3. The method for producing spore powder of Cordyceps sinensis according to claim 1, characterized in that: The diameter of the spherical culture medium is less than or equal to 1 cm.

4. The method for producing spore powder of Cordyceps sinensis according to claim 1, characterized in that: Step S2 specifically includes: The prepared culture medium is made into a spherical culture medium with a diameter of 0.6-1 cm by a pelleting machine; wherein, in order to prevent the outside from sticking, a thin layer of wheat bran powder is adhered to the outside of the spherical culture medium; Place the spheroid culture in a monolayer in a box for sterilization.

5. The method for producing spore powder of Cordyceps sinensis according to claim 1, characterized in that: In step S3, the inoculation amount of Cordyceps sinensis fungus is 5-10%.

6. The method for producing spore powder of Cordyceps sinensis according to claim 1, characterized in that: In step S3, the cultivation process is: Cultivate in the dark at 22-24°C for 7-8 days; After the mycelium of Cordyceps sinensis grows on the surface of the ball, light culture is carried out; the light culture intensity is 300-500 Lux, the temperature is 25-26°C, the humidity is controlled at 60-70%, the light time is 12-16h / d, and the culture time is 20-25 days.

7. The method for producing spore powder of Cordyceps sinensis according to claim 3, characterized in that: The spore powder yield of Cordyceps sinensis spore powder reaches 18-25%; wherein, the spore powder yield=spore powder dry weight / (spherical culture medium dry weight+spore powder dry weight)×100%.

8. A Cordyceps sinensis spore powder, characterized in that: The product is produced by the production method according to any one of claims 1 to 7.