SNP molecular markers related to wheat appearance quality and their applications

By developing SNP molecular markers and KASP primer combinations on wheat chromosome 2A, the problem of identifying wheat protein content in existing technologies has been solved, enabling rapid and accurate breeding selection and quality improvement.

CN120138194BActive Publication Date: 2026-05-26INSTITUTE OF CROP SCIENCE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
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Patent Information

Application Number
CN202510176617.7
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2025-02-18
Publication Date
2026-05-26
Estimated Expiration
2045-02-18

AI Technical Summary

Technical Problem

The lack of effective SNP markers in existing technologies for rapid and accurate identification of wheat protein content affects breeding efficiency and quality improvement.

Method used

A molecular marker for an SNP located at 767257977 bp on wheat chromosome 2A and its KASP primer combination were developed for rapid identification of protein content by PCR amplification. Specific primers were designed to detect polymorphic sites C or T.

Benefits of technology

It enables rapid and accurate identification of wheat protein content, improves breeding selection efficiency, shortens the breeding cycle, and allows for early prediction of quality traits, making it suitable for high-throughput detection.

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Abstract

This invention relates to the field of genetic engineering technology, and more particularly to a SNP molecular marker related to wheat appearance quality and its application. The SNP molecular marker described in this invention contains a nucleotide sequence with a polymorphism of C or T at position 767257977 bp on wheat chromosome 2A. This invention has determined that this site is a polymorphic site affecting grain length and protein content, and has designed a specific KASP primer combination to achieve rapid and accurate identification of wheat grain length and protein content.
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