Wearable cork wood-based aerogel sensor for rapidly detecting pseudomonas aeruginosa

Abstract

The invention discloses a wearable cork wood-based aerogel sensor for rapidly detecting pseudomonas aeruginosa, which is characterized in that cork wood aerogel is used as a carrier framework, and a PANI-In2O3 composite material is loaded on the carrier framework to obtain the cork wood-based aerogel sensor. And then assembling into the wearable cork wood-based aerogel sensor for rapidly detecting the pseudomonas aeruginosa in the wound surface. The purpose of instant detection is achieved by using a high-precision digital multimeter and combining with a computer terminal, and real-time rapid detection and timely early warning of wound infection with pseudomonas aeruginosa can be realized. The sensor disclosed by the invention has the characteristics of simplicity in operation, no need of complicated pretreatment steps, convenience in carrying, high sensitivity, low cost and the like, and has important significance on detecting the pseudomonas aeruginosa in real time and dynamically monitoring the infection degree of the pseudomonas aeruginosa in a wound surface.

Description

Technical Field

[0001] The present invention relates to the field of biosensing, and particularly to a wearable balsa wood-based aerogel sensor for rapid detection of Pseudomonas aeruginosa. Background Art

[0002] Infection of wounds with different pathogenic bacteria is a major cause endangering the lives of millions of people. Various related diseases may be caused by these pathogenic bacteria, such as bacteremia, sepsis, and multiple organ dysfunction syndrome, etc., which undoubtedly pose a huge threat to the life safety of patients. Conventional methods, such as culture, enzyme-linked immunosorbent assay (ELISA), and polymerase chain reaction (PCR), as well as some instrumental methods such as chemiluminescence, chromatography, and mass spectrometry, are used for bacterial detection. However, the above traditional detection methods are time-consuming, complex in operation, expensive in equipment, and cannot be monitored in real time, which limits their application scenarios.

[0003] At present, research reports have confirmed that after the wound is infected with Pseudomonas aeruginosa, it will produce specific volatile metabolites 1-undecene during the metabolic process. It is found that the presence of Pseudomonas aeruginosa can be indirectly identified by detecting 1-undecene. The determination of the characteristic VOC 1-undecene released by Pseudomonas aeruginosa not only helps to accurately and quickly predict whether the wound is infected with Pseudomonas aeruginosa, but also provides a novel idea for the detection method of real-time monitoring and controlling the degree of pathogenic bacteria infection in the wound, and expands the direction of real-time monitoring and timely warning of pathogenic bacteria infection in the wound. Therefore, efficient, rapid, and real-time tracking detection will be very beneficial for our timely warning and prevention of pathogenic bacteria infection in the wound.

[0004] In recent years, metal oxide semiconductor (MOS) gas sensors have been widely used in the design, manufacture, characterization, and application of sensors, providing a new basis for analysis and research. The MOS-type sensor consists of a sensing element with a sensing material coated on one side and a heater on the other side. When it works, the sensing material is heated to several hundred degrees Celsius, causing free electrons to flow through the material. When the sensing material is exposed to clean air, the material will be oxidized, resulting in a decrease in free electrons and an increase in the resistance of the sensing material. When exposed to reducing gases, the gas reacts with the adsorbed oxygen, releasing free electrons and reducing the resistance of the sensing material. However, the operation of the MOS-type sensor requires a working temperature of about 200 - 500 °C, which requires the installation of an additional heater, significantly increasing the power consumption and reducing the battery life. Moreover, it is greatly restricted by the test environment and does not have the advantages of low cost, good biocompatibility, inability to monitor in real time, and portability and wearability, which hinders their application in manufacturing applicable sensors. Summary of the Invention

[0005] The object of the present invention is to provide a wearable balsa wood-based aerogel sensor for rapid detection of Pseudomonas aeruginosa, which can be applied to the accurate prediction and timely prevention of wound infection with Pseudomonas aeruginosa.

[0006] To achieve the above object, the technical solution of the present invention is as follows:

[0007] A wearable balsa wood-based aerogel sensor for rapid detection of Pseudomonas aeruginosa, on which a gas-sensitive response material PANI-In 2 O 3 composite material is loaded to obtain a balsa wood-based aerogel sensor loaded with PANI-In 2 O 3 composite material, and then it is designed and self-assembled to obtain a portable wearable balsa wood-based aerogel sensor.

[0008] The preparation method of the balsa wood aerogel skeleton includes:

[0009] First, a 15mm×10mm×5mm balsa wood sample is placed in a mixed aqueous solution of 2.5mol / L NaOH, 0.4mol / L NaSO 3 and 4.9mol / L CH 3 OH, and treated at 120°C for 12h. After 12h, the sample is taken out and rinsed repeatedly with deionized water for more than 3 times.

[0010] Subsequently, the sample obtained in the first step is placed in a mixed aqueous solution of 2.5mol / L H 2 O 2 and treated multiple times at 80°C until the wood turns white. After taking out the sample, it is rinsed repeatedly with deionized water for more than 3 times.

[0011] Finally, the sample obtained in the second step is placed in a vacuum freeze dryer and dried for 48h to prepare a white balsa wood aerogel.

[0012] The preparation method of the balsa wood-based aerogel sensor loaded with PANI-In 2 O 3 composite material includes:

[0013] First, 1ml of aniline monomer solution and a certain proportion of indium oxide nanoparticles are added to 30mL of a 1mol / L hydrochloric acid solution, and ultrasonic treatment is carried out for 30min, and pre-cooled for a period of time to obtain solution 1.

[0014] Secondly, 2.5g of ammonium persulfate APS is dissolved in 30ml of a 1mol / L hydrochloric acid solution, and then the above solution is ultrasonically treated for 30min and pre-cooled for a period of time to obtain solution 2.

[0015] Subsequently, the balsa wood-based aerogel skeleton was immediately immersed in the above-mentioned solution 2, ultrasonic-treated for 30 min, and pre-cooled for a period of time to obtain a solution 3 containing the balsa wood-based aerogel skeleton.

[0016] Finally, solution 1 containing aniline monomer and a certain proportion of indium oxide nanoparticles was rapidly dropped into solution 3 containing the balsa wood-based aerogel skeleton, and a polymerization reaction was carried out in an ice-water bath for 3 h. During this period, it was gently stirred with a glass rod. After the reaction was completed, the prepared sample was repeatedly washed in deionized water for many times, and then dried in a vacuum freeze dryer for 48 h to obtain a balsa wood-based aerogel sensor loaded with PANI-In 2 O 3 composite material.

[0017] The preparation method of the wearable balsa wood-based aerogel sensor loaded with PANI-In 2 O 3 composite gas-sensitive material includes:

[0018] First, the common wearable sensor flexible material polyethylene terephthalate (PET) was selected as the flexible substrate. Using conductive copper tape and copper wire, the above-prepared balsa wood-based aerogel sensor loaded with PANI-In 2 O 3 composite material was fixed and installed on the PET flexible substrate.

[0019] Secondly, a semi-closed cavity body of a certain size was designed and manufactured using the flexible substrate PET, with the opening part facing downwards so as to be able to be on the wound surface 1-undecene.

[0020] Finally, the balsa wood-based aerogel sensor loaded with PANI-In 2 O 3 composite material installed on the PET substrate in the first step was self-assembled with the semi-closed cavity body through conductive tape and conductive silver paste to obtain a portable wearable balsa wood-based aerogel sensor.

[0021] The present invention also provides a preparation method of a wearable balsa wood-based aerogel sensor for rapid detection of Pseudomonas aeruginosa, including:

[0022] Preparing a balsa wood aerogel skeleton by chemical treatment, and then preparing a balsa wood-based aerogel sensor loaded with PANI-In 2 O 3 composite material, and then combining the balsa wood-based aerogel sensor loaded with PANI-In 2 O 3 composite material with the flexible substrate PET by self-assembly to manufacture a portable wearable balsa wood-based aerogel sensor.

[0023] The present invention also provides an application of a wearable balsa-based aerogel sensor for rapid detection of Pseudomonas aeruginosa, and the wearable balsa-based aerogel sensor is applied to a data acquisition system for detecting the signature volatile metabolite 1-undecene released by Pseudomonas aeruginosa.

[0024] The data acquisition system mainly includes: a sensor element, copper wires, a high-precision digital multimeter, and a computer terminal; both ends of the sensor element are connected to the copper wires, the copper wires are connected to the high-precision digital multimeter, and the high-precision digital multimeter is connected to the computer terminal.

[0025] The principle of the present invention is as follows:

[0026] Screen out PANI-In 2 O 3 The composite material is used as a gas-sensitive element with specific response to the characteristic marker 1-undecene gas molecules of Pseudomonas aeruginosa. The PANI-In 2 O 3 The composite material is in-situ loaded on the balsa aerogel skeleton and self-assembled to construct a wearable balsa-based aerogel chemiresistor sensor for room-temperature detection, which has the advantages of low power consumption, high sensitivity, portability, and real-time detection.

[0027] By selecting balsa aerogel as the carrier skeleton, due to the three-dimensional porous structure and high specific surface area of balsa aerogel, a large number of active sites are provided, which is very conducive to improving the sensing performance of detection.

[0028] Due to the excellent specific response ability of the screened PANI-In 2 O 3 The composite material to the target gas 1-undecene, it overcomes the disadvantages of insufficient specific recognition ability, low sensitivity, and unstable structure of most current gas sensing devices. Using a high-precision digital multimeter and combining with a computer terminal achieves the purpose of instant detection. The sensor of the present invention has good stability and is used for real-time monitoring of pathogenic bacteria in wound infections, which helps to reduce the health risks brought by pathogenic bacteria to humans.

[0029] The beneficial effects of the present invention are as follows:

[0030] 1. In the present invention, the prepared wearable balsa-based aerogel sensor loaded with PANI-In 2 O 3 The composite material is installed at the wound site on the back of a mouse infected with Pseudomonas aeruginosa. When it is exposed to the characteristic volatile metabolite 1-undecene gas atmosphere released by Pseudomonas aeruginosa, rapid and accurate identification and detection of Pseudomonas aeruginosa in the wound can be achieved. The detection time is several minutes, and the effect of real-time detection can be achieved.

[0031] 2. The sensor of the present invention is simple to prepare and operate, does not require complex pretreatment, has low cost, is convenient to carry, and has high sensitivity and stability.

[0032] 3. The present invention selects balsa wood aerogel as the carrier skeleton. Due to the three-dimensional porous structure and high specific surface area of the balsa wood aerogel, a large number of reactive sites for adsorbing target gases are provided, which is very beneficial to improving the detection and sensing performance of the sensor. In addition, the selected PANI-In 2 O 3 composite gas-sensitive material has excellent specific response ability to the target gas 1-undecene, which overcomes the disadvantages of insufficient specific recognition ability, low sensitivity, and unstable structure of most current gas sensing devices. All in all, the present invention is of great significance for the real-time detection and dynamic monitoring of wound infection with Pseudomonas aeruginosa. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 is the flow chart of the present invention;

[0034] Figure 2 is the flow chart for preparing the balsa wood aerogel skeleton;

[0035] Figure 3 are the physical and SEM images of balsa wood and the balsa wood aerogel skeleton;

[0036] Figure 4 is the flow chart for preparing the balsa wood-based aerogel loaded with PANI-In 2 O 3 composite material;

[0037] Figure 5 is the SEM image of the balsa wood-based aerogel loaded with PANI-In 2 O 3 composite material provided by the present invention;

[0038] Figure 6 is the physical image of the balsa wood-based aerogel loaded with PANI-In 2 O 3 composite material provided by the present invention;

[0039] Figure 7 is the structural schematic diagram of the wearable balsa wood-based aerogel sensor;

[0040] Figure 8 is the schematic diagram of the data acquisition system;

[0041] Figure 9 is the response and recovery curve graph of the sensor detecting different concentrations of 1-undecene, the characteristic volatile substance of Pseudomonas aeruginosa, from 1 to 100 ppm;

[0042] Figure 10It is a graph of the actual monitoring results of a wearable balsa-based aerogel sensor for wound infection with Pseudomonas aeruginosa in mice. Detailed implementation manners

[0043] The present invention will be further described below in conjunction with specific embodiments. The following are the implementation examples of the present invention, which are not intended to limit the present invention. Any modifications, substitutions, improvements, etc. made on the basis of the present invention are all included in the protection scope of the present invention. In the following examples, the experimental methods are all conventional methods unless otherwise specified; the experimental materials used are all conventional biochemical reagents unless otherwise specified, and can be obtained through commercial channels.

[0044] Example 1

[0045] This example relates to the preparation of a balsa aerogel skeleton.

[0046] Put a 15mm×10mm×5mm balsa wood sample into a mixed aqueous solution of 2.5mol / L NaOH, 0.4mol / L NaSO 3 and 4.9mol / LCH 3 OH, and treat it at 120°C for 12h. After 12h, take out the sample and rinse it repeatedly with deionized water for more than 3 times.

[0047] Subsequently, put the sample obtained in the first step into a mixed aqueous solution of 2.5mol / L H 2 O 2 , and treat it multiple times at 80°C until the wood turns white. After taking out the sample, rinse it repeatedly with deionized water for more than 3 times.

[0048] Finally, place the sample obtained in the second step in a vacuum freeze dryer and dry it for 48h to prepare a white balsa-based aerogel. The preparation route is as Figure 2 shown, and the SEM images of balsa wood and balsa-based aerogel samples are as Figure 3 shown.

[0049] Example 2

[0050] This example relates to the preparation of a balsa-based aerogel sensor loaded with PANI-In 2 O 3 composite gas-sensitive material.

[0051] First, add 1ml of aniline monomer solution and a certain proportion of indium oxide nanoparticles to 30mL of a hydrochloric acid solution with a concentration of 1mol / L, and ultrasonically treat it for 30min. After pre-cooling for a period of time, solution 1 is obtained.

[0052] Secondly, 2.5 g of ammonium persulfate (APS) was dissolved in 30 ml of hydrochloric acid solution with a concentration of 1 mol / L. Immediately, the above solution was ultrasonically treated for 30 min and pre-cooled for a period of time to obtain Solution 2.

[0053] Subsequently, the delignified balsa wood was immediately immersed in the above Solution 2 and ultrasonically treated for 30 min and pre-cooled for a period of time to obtain Solution 3 containing the delignified balsa wood.

[0054] Finally, Solution 1 containing aniline monomer and a certain proportion of indium oxide nanoparticles was rapidly added dropwise to Solution 3 containing the delignified balsa wood. The polymerization reaction was carried out in an ice-water bath for 3 h, and during this period, it was gently stirred with a glass rod. After the reaction was completed, the prepared sample was repeatedly washed in deionized water for many times, and then placed in a vacuum freeze dryer for drying for 48 h to obtain the balsa wood-based aerogel sensor loaded with PANI-In 2 O 3 composite material. The preparation process is as Figure 4 shown. The physical picture and SEM image of the balsa wood-based aerogel sample loaded with PANI-In 2 O 3 composite material are as shown in Figure 5 、 5 respectively.

[0055] Example 3

[0056] This example relates to the fabrication of a wearable balsa wood-based aerogel sensor loaded with PANI-In 2 O 3 composite gas-sensitive material.

[0057] First of all, the common wearable sensor flexible material polyethylene terephthalate (PET) was selected as the flexible substrate. Using conductive copper tape and copper wire, the above-prepared balsa wood-based aerogel sensor loaded with PANI-In 2 O 3 composite material was fixed and installed on the PET flexible substrate.

[0058] Secondly, a closed cavity body of a certain size was designed and fabricated using the flexible substrate PET.

[0059] Finally, the balsa wood-based aerogel sensor loaded with PANI-In 2 O 3 composite material installed on the PET substrate in the first step was self-assembled with the closed cavity body fabricated in the second step through conductive tape and conductive silver paste to obtain a portable wearable balsa wood-based aerogel sensor. The gas-sensing performance of the sensor was tested using a self-built static platform. Before the first measurement, the sensor was placed in room-temperature air until the baseline was stable. The response signal was defined as:

[0060]

[0061] Wherein: R g and R 0 are respectively the resistances of the sensor when exposed to the sample gas or air.

[0062] The structural diagram of the sensor manufacturing is as shown in Figure 7 the figure.

[0063] Example 4

[0064] This example relates to the design of the data acquisition system.

[0065] Using a high-precision digital multimeter as the electrical signal data acquisition system of the wearable balsa-based aerogel sensor of the present invention, aiming to collect, display, and save the electrical signals of the sensor in real time. The overall structural schematic diagram of the data acquisition system is as shown in Figure 8 the figure. It mainly includes a wearable balsa-based aerogel sensor, a flow control valve, an air bag, a flow meter, a test bottle, a high-precision digital multimeter, copper wires, and a computer terminal. After completing the acquisition of an electrical signal, the data is packed and sent to the computer through a USB serial port. The function of the computer is to parse the data packet and display and save the data. The response recovery curve diagrams of different concentrations of 1-undecene gas, a characteristic marker released by Pseudomonas aeruginosa, from 1 to 100 ppm are as shown in Figure 9 the figure.

[0066] Example 5

[0067] This example relates to the practical application of the wearable balsa-based aerogel sensor for wound infection with Pseudomonas aeruginosa.

[0068] Select mice as the research object. First, establish a wound infection model for mice. After anesthetizing the mice, shave and disinfect the back, and then use a professional scissors to cut a circular wound of a certain size along the back. Then, drop a suspension of Pseudomonas aeruginosa at a certain concentration onto the wound surface to establish a wound infection model for mice. After inoculating bacteria on the wound surface of the mice, install the wearable balsa-based aerogel sensor of the present invention at the wound infected with Pseudomonas aeruginosa, record the time of inoculating the bacteria, and observe the change of the response value of the sensor. The real-time monitoring results are as shown in Figure 10 the figure. The wound of the mice infected with Pseudomonas aeruginosa was continuously monitored for 7.5 days. The results confirmed that the wearable balsa-based aerogel sensor of the present invention can effectively monitor in the early stage of pathogen infection and can accurately and quickly identify the types of pathogens, showing great potential in the field of wound infection pathogen monitoring.

[0069] Matters not described in the present invention are well-known technologies.

[0070] The above embodiments are only used to illustrate the technical concept and features of the present invention. The purpose is to enable those skilled in the art to understand the content of the present invention and implement it accordingly, and it should not be used to limit the protection scope of the present invention. Any equivalent changes or modifications made according to the spirit and essence of the present invention should be covered within the protection scope of the present invention.

Claims

1. A light wood-based composite material, characterized in that: Balsa wood aerogel was used as the carrier skeleton and PANI-In2O3 composite material was loaded on it.

2. Use of the balsa wood-based composite material according to claim 1 in the preparation of a sensor for detecting Pseudomonas aeruginosa.

3. A sensor for detecting Pseudomonas aeruginosa, characterized in that: The sensor for detecting Pseudomonas aeruginosa has a semi-enclosed cavity with an opening at the bottom. The balsa wood aerogel loaded with the PANI-In2O3 composite material according to claim 1 is fixed at the top of the semi-enclosed cavity, and wires are connected at both ends of the balsa wood aerogel loaded with the PANI-In2O3 composite material.

4. The balsa wood-based composite material according to claim 1, characterized in that: The balsa aerogel skeleton is prepared by the following method: The balsa wood samples were placed in a mixed aqueous solution of 2.5 mol / L NaOH, 0.4 mol / L NaSO3 and 4.9 mol / L CH3OH and treated at 120°C; after treatment, the samples were taken out and repeatedly rinsed with deionized water; The obtained sample was placed in a mixed aqueous solution of 2.5 mol / L H2O2 and treated multiple times at 80°C until the wood turned white. After the sample was taken out, it was repeatedly rinsed with deionized water. The obtained sample was placed in a vacuum freeze dryer and dried for 48 hours to prepare a white balsa wood aerogel skeleton.

5. The balsa wood-based composite material according to claim 1, characterized in that: The preparation method of the balsa wood-based aerogel sensor loaded with PANI-In2O3 composite material is as follows: 1 ml of aniline monomer solution and a certain proportion of indium oxide nanoparticles were added to 30 mL of 1 mol / L hydrochloric acid solution, ultrasonically treated, and precooled for a period of time to obtain solution 1; 2.5 g of ammonium persulfate APS was dissolved in 30 ml of 1 mol / L hydrochloric acid solution, and then the solution was ultrasonically treated and precooled for a period of time to obtain solution 2; The balsa wood aerogel is immediately immersed in the above solution 2, subjected to ultrasonic treatment, and precooled for a period of time to obtain solution 3; Solution 1 containing aniline monomer and a certain proportion of indium oxide nanoparticles was quickly added dropwise to solution 3, and polymerization reaction was carried out in an ice water bath, during which time a glass rod was used to gently stir; after the reaction was completed, the prepared sample was placed in deionized water and washed repeatedly for several times, and then placed in a vacuum freeze dryer to obtain a light wood-based aerogel sensor loaded with PANI-In2O3 composite material.

6. The sensor for detecting Pseudomonas aeruginosa according to claim 3, characterized in that: The sensor for detecting Pseudomonas aeruginosa adopts flexible polyethylene terephthalate (PET) as a flexible substrate, and a light wood-based aerogel sensor loaded with a PANI-In2O3 composite material is fixed and installed on the PET flexible substrate.

7. A data acquisition system for rapid detection of Pseudomonas aeruginosa, characterized in that: It includes a high-precision digital multimeter, a computer terminal and the sensor for detecting Pseudomonas aeruginosa as described in claim 3, and the specific positions and connection relationships are as follows: The target gas and nitrogen are stored in gas storage tanks respectively. The target gas and nitrogen gas storage tanks are respectively connected to a gas supply pipeline after being controlled by gas valves. A flow meter and a control valve are arranged on the gas supply pipeline. The gas supply pipeline is connected to a test bottle. The test bottle is provided with a gas outlet. The Pseudomonas aeruginosa detection sensor is arranged in the test bottle and connected to a digital multimeter through a wire. The digital multimeter is connected to a computer. The electrical signal of the Pseudomonas aeruginosa detection sensor is obtained through the digital multimeter and the electrical signal is sent to the computer, and the computer processes and obtains data information.

8. Use of the data acquisition system for rapid detection of Pseudomonas aeruginosa according to claim 7 in detecting 1-undecene, a characteristic volatile metabolite released by Pseudomonas aeruginosa.

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