Pharmaceutical composition for preventing and treating fungal diseases of tobaccos and preparation method of pharmaceutical composition

Abstract

The invention provides a pharmaceutical composition for preventing and treating fungal diseases of tobaccos, and belongs to the technical field of pesticides. The pharmaceutical composition for preventing and treating fungal diseases of tobaccos comprises the following raw materials: plant extract components, a compound microbial agent, eugenol, an emulsifier, a thickener and water. The pharmaceutical composition disclosed by the invention mainly comprises the plant extract component, the compound microbial agent and the eugenol, and the components are purely natural, green, environment-friendly and low in cost. The Chinese herbaceous peony extract, the fructus forsythiae extract, the cortex cinnamomi extract and the radix angelicae pubescentis extract are mixed for use, the trichoderma harzianum and the bacillus velezensis are mixed for use, the plant extract components, the compound microbial agent and the eugenol are compounded for use, and the various components have a synergistic effect; the prevention effect of the pharmaceutical composition on tobacco brown spot, black shank and root black rot is obviously improved.

Description

Technical Field

[0001] The present invention relates to the technical field of pesticides, and in particular to a pharmaceutical composition for preventing and treating tobacco fungal diseases and a preparation method thereof. Background Art

[0002] Tobacco is an important cash crop and an important raw material for the cigarette industry. Tobacco cultivation is the basis of the tobacco industry. The damage caused by tobacco pests and diseases has a great impact on the quality and yield of tobacco, seriously affecting the sustainable development of the tobacco industry.

[0003] The prevention and control of tobacco diseases mainly include chemical control and biological control. Chemical pesticides have good effects in a short time, but are prone to cause drug resistance and drug residues, and have a greater impact on the ecological environment. The biological control of tobacco black shank mainly includes the use of bacteria, actinomycetes and fungal biocontrol bacteria, the screening of cell mutants and the induction of improving the resistance of tobacco to tobacco black shank. For example, the bacteria with antagonistic effects on tobacco black shank bacteria are Bacillus and Pseudomonas, which mainly dissolve the mycelia of tobacco black shank bacteria by producing antibiotics. Using biological control to prevent and treat tobacco black shank can not only protect the environment, but also prevent black shank from developing drug resistance and cause no harm to the plants themselves. However, the biological control method has high costs, complex preparation methods, and cannot show effects in a short time.

[0004] Seeking an environmentally friendly, fast-acting and long-lasting method for preventing and treating tobacco fungal diseases is the main problem to be solved at present.

[0005] For example, Chinese Patent CN106538530B discloses an eugenol aqueous emulsion, a preparation method thereof and an application in preventing and treating tobacco fungal diseases. The raw materials of the eugenol aqueous emulsion include 20-30% of eugenol, 1-5% of emulsifier, 0.1-0.3% of thickener, 3-5% of antifreeze, 0.1-0.5% of defoamer and the balance of water. Using the eugenol aqueous emulsion to prevent and treat tobacco black shank and tobacco brown spot has a high inhibitory effect on both tobacco black shank bacteria and tobacco brown spot.

[0006] However, the highest inhibition rate of the above method on brown spot bacteria is only 65.67%, and the effect is poor.

[0007] It can be seen that fungal diseases in the process of tobacco cultivation have always been a prominent problem. The traditional chemical pesticide control has a negative impact on the production of sucking cash crops. Biological control has always been the focus of the industry. The traditional biological control agents for tobacco fungal diseases generally have the characteristics of low effect and slow onset. Exploring and developing innovative biocontrol technologies for tobacco fungal diseases is still an urgent problem to be solved in the tobacco industry and a strategic demand technology. Summary of the Invention

[0008] In view of this, the object of the present invention is to provide a drug composition for preventing and controlling tobacco fungal diseases, which is simple to prepare, green and environmentally friendly, low in cost, pure natural, and has broad-spectrum properties.

[0009] To achieve the above object, the present invention provides a drug composition for preventing and controlling tobacco fungal diseases, comprising the following raw materials:

[0010] Plant extraction components, compound microbial inoculum, eugenol, emulsifier, thickener, water.

[0011] Preferably, the drug composition for preventing and controlling tobacco fungal diseases comprises the following raw materials in parts by weight:

[0012] 10-15 parts of plant extraction components, 2-5 parts of compound microbial inoculum, 2-5 parts of eugenol, 0.5-0.8 part of emulsifier, 0.1-0.5 part of thickener, 60-100 parts of water.

[0013] Preferably, the plant extraction components comprise the following raw materials in parts by weight: 1-3 parts of paeony extract, 2-5 parts of forsythia extract, 1-3 parts of cinnamon extract, 2-5 parts of angelica pubescens extract.

[0014] Preferably, the compound microbial inoculum comprises the following raw materials in parts by weight: 2-3 parts of trichoderma harzianum, 3-4 parts of bacillus velezensis.

[0015] Preferably, the emulsifier is any one or more of tween-20, tween-80 or tween-60.

[0016] Preferably, the thickener is any one or more of xanthan gum, magnesium aluminum silicate, gum arabic.

[0017] Another object of the present invention is to provide a preparation method of a drug composition for preventing and controlling tobacco fungal diseases, the preparation method comprising the following steps:

[0018] S1: Preparation of paeony extract: Crush the dried paeony, mix it with absolute ethanol and soak, then extract under ultrasonic conditions, centrifuge to obtain the supernatant, mix the supernatant with absolute ethanol and soak, perform reflux extraction, refrigerate and precipitate, rotary evaporate to remove the solvent, and then perform vacuum freeze-drying to obtain paeony extract;

[0019] S2: Preparation of forsythia extract: Add the dried forsythia leaves to water and decoct, filter to obtain the medicinal liquid, add water to the medicinal residues and decoct again, filter to obtain the medicinal liquid, combine the two medicinal liquids and concentrate; then add absolute ethanol and soak in the refrigerator, filter, heat and reflux extract the filtrate, rotary evaporate to remove ethanol, and dry to obtain forsythia extract;

[0020] S3: Preparation of cinnamon extract: Cinnamon is mixed with water and soaked to obtain a soaking extract and cinnamon residues; ethanol is added to the cinnamon residues for reflux extraction to obtain a reflux extract; the soaking extract and the reflux extract are combined, concentrated and dried to obtain the cinnamon extract;

[0021] S4: Preparation of angelica pubescens extract: The dried angelica pubescens is crushed, ethanol is added for oscillating extraction, filtered, the filtrate is collected, then the filtrate is centrifuged, the supernatant is collected, the solvent in the supernatant is removed by rotary evaporation and dried to obtain the angelica pubescens extract;

[0022] S5: The paeony extract, forsythia extract, cinnamon extract and angelica pubescens extract are mixed to obtain a plant extract component;

[0023] S6: Trichoderma harzianum and Bacillus velezensis are mixed to obtain a compound microbial inoculum;

[0024] S7: The plant extract component and the compound microbial inoculum are added to water to obtain a mixed solution, then eugenol, an emulsifier and a thickener are added and stirred evenly to obtain the pharmaceutical composition for preventing and treating tobacco fungal diseases.

[0025] Preferably, the rotation speed of the stirring in step S7 is 700 - 800 r / min, and the stirring time is 5 - 10 min.

[0026] Application of the pharmaceutical composition for preventing and treating tobacco fungal diseases according to the present invention, the pharmaceutical composition is used for preventing and treating tobacco brown spot, black shank and root black rot.

[0027] Preferably, the application of the pharmaceutical composition for preventing and treating tobacco fungal diseases includes the following steps: The compound microbial inoculum is configured with water into a mixed solution with a concentration of 1.0 wt% - 1.5 wt%, and evenly spread on the surface of moist soil during the seedling stage of tobacco.

[0028] Compared with the prior art, the present invention has the following beneficial effects:

[0029] The present invention provides a pharmaceutical composition for preventing and treating tobacco fungal diseases and a preparation method thereof. The pharmaceutical composition includes a plant extract component, a compound microbial inoculum, eugenol, an emulsifier, a thickener and water.

[0030] In the present invention, the plant extract component is obtained by compounding paeony extract, forsythia extract, cinnamon extract, and angelica pubescens extract. The paeony extract contains a large amount of paeoniflorin, which has an inhibitory effect on certain pathogenic bacteria and fungi; the forsythia extract contains triterpenoid saponins, and the pericarp contains sterols, phenolic components (forsythol), alkaloids, saponins, oleanolic acid, coumarin derivatives, as well as rich vitamins and a small amount of volatile oil, having a broad-spectrum antibacterial effect; both the cinnamon extract and the angelica pubescens extract have obvious bactericidal effects. The above components are used in combination to improve the antibacterial effect.

[0031] The compound microbial inoculum of the present invention is composed of trichoderma harzianum and bacillus velezensis, and the combination of the two components improves the inhibitory effect on the growth of harmful fungi.

[0032] The plant extract component, compound microbial inoculum, and eugenol of the present invention are used in combination, and the synergistic effect of multiple components significantly improves the control effects of the pharmaceutical composition on tobacco brown spot, black shank, and root black rot. Moreover, the main components of the present invention are natural, environmentally friendly, and low-cost. Brief Description of the Drawings

[0033] Figure 1 It is the control effect results of the pharmaceutical composition on tobacco brown spot, black shank, and root black rot after 40 days of spraying in Examples 1 to 3 of the present invention. Detailed Description of the Invention

[0034] The present invention provides a pharmaceutical composition for preventing and treating tobacco fungal diseases and a preparation method thereof.

[0035] The pharmaceutical composition for preventing and treating tobacco fungal diseases according to the present invention is composed of the following raw materials in parts by weight:

[0036] 10 - 15 parts of plant extract component, 2 - 5 parts of compound microbial inoculum, 2 - 5 parts of eugenol, 0.5 - 0.8 parts of emulsifier, 0.1 - 0.5 parts of thickener, and 60 - 100 parts of water.

[0037] In the specific embodiments of the present invention, eugenol is a raw material purchased conventionally in the market.

[0038] The plant extract component described in the present invention is composed of the following raw materials in parts by weight: 1 - 3 parts of paeony extract, 2 - 5 parts of forsythia extract, 1 - 3 parts of cinnamon extract, and 2 - 5 parts of angelica pubescens extract.

[0039] The compound microbial inoculum includes the following raw materials in parts by weight: 2 - 3 parts of trichoderma harzianum and 3 - 4 parts of bacillus velezensis. In the specific embodiments of the present invention, the trichoderma harzianum is a raw material purchased conventionally in the market, and the effective viable count in the trichoderma powder is 15 billion / g. The bacillus velezensis is purchased from the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC AB 2018297;

[0040] Preferably, the emulsifier is any one or more of Tween-20, Tween-80 or Tween-60.

[0041] Preferably, the thickener is any one or more of xanthan gum, magnesium aluminum silicate, and gum arabic.

[0042] The preparation method of the pharmaceutical composition for preventing and treating tobacco fungal diseases in the specific embodiments of the present invention is as follows:

[0043] S1: Preparation of paeony extract: Crush the dried paeony, pass through a 100-mesh sieve, then mix it with absolute ethanol in a ratio of 1 g: 10 mL and soak for 5 h, then extract under ultrasonic conditions for 20 min, centrifuge to obtain the supernatant, mix the supernatant with absolute ethanol in a volume ratio of 1:2, soak for 1 h, reflux and extract at 45 °C for 2 h, refrigerate at 4 °C for 2 h to precipitate and then filter, rotary evaporate to remove the solvent, and obtain the paeony extract through vacuum freeze-drying; wherein the ultrasonic power is 450 W;

[0044] S2: Preparation of forsythia extract: According to the ratio of forsythia leaves to water of 1 g: 12 mL, add the dried forsythia leaves to water and decoct for 2 h, filter to obtain the medicinal liquid, then add water to the medicinal residues according to the ratio of medicinal residues to water of 1 g: 10 mL and decoct for 2 h, filter to obtain the medicinal liquid, combine the two medicinal liquids, and concentrate to a relative density of 1.02 - 1.10 at 60 °C; then add absolute ethanol until the ethanol content is 70%, soak and refrigerate at 4 °C for 15 h, filter, heat and reflux extract the filtrate at 85 °C and then rotary evaporate to remove ethanol, and obtain the forsythia extract through freeze-drying;

[0045] S3: Preparation of cinnamon extract: According to the ratio of cinnamon to water of 1 g: 10 mL, mix the dried cinnamon with water and soak for 12 h to obtain the soaking extract and cinnamon residues; add an ethanol solution with a volume fraction of 80% to the cinnamon residues according to the ratio of cinnamon residues to the ethanol solution of 1 g: 11 mL, reflux and extract at 85 °C to obtain the reflux extract; combine the soaking extract and the reflux extract, concentrate, and obtain the cinnamon extract through freeze-drying;

[0046] S4: Preparation of angelica pubescens extract: Crush the dried angelica pubescens, pass through a 100-mesh sieve to obtain angelica pubescens powder, then add an ethanol solution with a volume fraction of 80% according to the ratio of angelica pubescens powder to the ethanol solution of 1 g: 8 mL, oscillate and extract at 150 r / min for 12 h, filter, collect the filtrate, then centrifuge at 6000 r / min for 5 min, collect the supernatant, rotary evaporate to remove the solvent in the supernatant, and dry to obtain the angelica pubescens extract;

[0047] S5: Mix the paeony extract, forsythia extract, cinnamon extract, and angelica pubescens extract in proportion to obtain the plant extraction component;

[0048] S6: Mix Trichoderma harzianum and Bacillus velezensis in proportion to obtain a compound microbial inoculum;

[0049] S7: Add the plant extract component and the compound microbial inoculum into water to obtain a mixed solution, then add eugenol, an emulsifier, and a thickener, and stir at 800 r / min for 5 min to uniformly mix the raw materials, thereby obtaining the pharmaceutical composition for preventing and treating tobacco fungal diseases.

[0050] The present invention will be further described below in conjunction with embodiments.

[0051] Example 1

[0052] A pharmaceutical composition for preventing and treating tobacco fungal diseases is composed of the following raw materials in parts by weight:

[0053] 10 parts of plant extract component, 2 parts of compound microbial inoculum, 4 parts of eugenol, 0.5 part of emulsifier, 0.2 part of thickener, and 60 parts of water;

[0054] The plant extract component is composed of the following raw materials in parts by weight: 1 part of Paeonia lactiflora extract, 2 parts of Forsythia suspensa extract, 2 parts of Cinnamomum cassia extract, and 5 parts of Angelica pubescens extract.

[0055] The compound microbial inoculum includes the following raw materials in parts by weight: 3 parts of Trichoderma harzianum and 3 parts of Bacillus velezensis;

[0056] The emulsifier is Tween-20; the thickener is xanthan gum.

[0057] Example 2

[0058] A pharmaceutical composition for preventing and treating tobacco fungal diseases is composed of the following raw materials in parts by weight:

[0059] 13 parts of plant extract component, 4 parts of compound microbial inoculum, 3 parts of eugenol, 0.5 part of emulsifier, 0.3 part of thickener, and 70 parts of water;

[0060] The plant extract component is composed of the following raw materials in parts by weight: 3 parts of Paeonia lactiflora extract, 2 parts of Forsythia suspensa extract, 2 parts of Cinnamomum cassia extract, and 5 parts of Angelica pubescens extract.

[0061] The compound microbial inoculum includes the following raw materials in parts by weight: 3 parts of Trichoderma harzianum and 4 parts of Bacillus velezensis;

[0062] The emulsifier is Tween-20; the thickener is magnesium aluminum silicate.

[0063] Example 3

[0064] A pharmaceutical composition for preventing and treating tobacco fungal diseases is composed of the following raw materials in parts by weight:

[0065] 12 parts of plant extracts, 5 parts of compound microbial inoculant, 2 parts of eugenol, 0.6 part of emulsifier, 0.4 part of thickener, and 65 parts of water;

[0066] The plant extracts are composed of the following raw materials in parts by weight: 2 parts of peony extract, 3 parts of forsythia extract, 2 parts of cinnamon extract, and 4 parts of angelica pubescens extract.

[0067] The compound microbial inoculant includes the following raw materials in parts by weight: 2 parts of trichoderma harzianum and 3 parts of bacillus velezensis;

[0068] The emulsifier is Tween-80; the thickener is xanthan gum.

[0069] The pharmaceutical compositions prepared in Examples 1 to 3 are formulated with water into a mixed solution with a concentration of 1.0 wt% for preventing and controlling tobacco fungal diseases. The application amount per plant is 0.2 L, and it is evenly spread on the surface of moist soil during the tobacco seedling stage, and then the prevention and control of tobacco fungal diseases can be achieved.

[0070] 30 days and 60 days after spraying the medicament, record the growth conditions of tobacco using different mixed solutions, and calculate the control effects on tobacco brown spot, black shank, and root black rot. The results are shown in Table 1. The control effect results on tobacco brown spot, black shank, and root black rot 40 days after spraying are as follows Figure 1 .

[0071] Table 1

[0072]

[0073] As can be seen from Table 1, using the pharmaceutical compositions prepared in Examples 1 to 3 of the present invention, 30 days and 60 days after spraying the medicament, it has a high control effect on tobacco brown spot, black shank, and root black rot, and the pharmaceutical composition of the present invention has a long-term effect.

[0074] Change the raw materials of Example 1 to prepare a pharmaceutical composition for preventing and controlling tobacco fungal diseases, and formulate the pharmaceutical composition with the changed formula and water into a mixed solution with a concentration of 1.0 wt% for preventing and controlling tobacco fungal diseases. The application amount per plant is 0.2 L, and it is evenly spread on the surface of moist soil during the tobacco seedling stage for the prevention and control of tobacco fungal diseases. Record the control effect 30 days after spraying the medicament. The specific raw material changes and control effects are shown in Comparative Examples 1 to 3.

[0075] Comparative Example 1

[0076] Adjust the plant extracts to prepare a pharmaceutical composition for preventing and controlling tobacco fungal diseases. The other raw material compositions are the same as those in Example 1. The specific component contents and control effects are shown in Table 2.

[0077] Table 2

[0078]

[0079]

[0080] The plant extracts include Paeonia lactiflora extract, Forsythia suspensa extract, Cinnamomum cassia extract and Angelica pubescens extract. It can be seen from Table 1 and Table 2 that when only one of these components is added, the control effect on tobacco fungal diseases remains almost unchanged or even slightly decreases; when 3 components are added, the control effect slightly increases, but when all of the above four components are added, the control effect significantly increases, proving that the above 4 components have a synergistic effect, significantly improving the control effect of the pharmaceutical composition on tobacco fungal diseases.

[0081] Comparative Example 2

[0082] Adjust the components of the compound microbial inoculant to prepare a pharmaceutical composition for controlling tobacco fungal diseases. The other raw material compositions are the same as those in Example 1. The specific component contents and control effects are shown in Table 3.

[0083] Table 3

[0084] Composition of compound microbial inoculum Control effect of tobacco brown spot (%) Control effect of black shank (%) Control effect of root black rot (%) Not added 77 79 71 2 parts of Trichoderma harzianum 81 83 79 3 parts of Bacillus velezensis 84 82 78

[0085] The compound microbial inoculant includes Trichoderma harzianum and Bacillus velezensis. It can be seen from Table 1 and Table 3 that when only one of these components is added, the control effect on tobacco fungal diseases slightly increases, but when both of the above two components are added, the control effect significantly increases, proving that the above 2 components have a synergistic effect, significantly improving the control effect of the pharmaceutical composition on tobacco fungal diseases.

[0086] Comparative Example 3

[0087] Adjust the addition amount of eugenol to prepare a pharmaceutical composition for controlling tobacco fungal diseases. The other raw material compositions are the same as those in Example 1. The specific component contents and control effects are shown in Table 4.

[0088] Table 4

[0089]

[0090]

[0091] It can be seen from the examples and comparative examples of the present invention that there is a synergistic relationship among the plant extracts, the compound microbial inoculant and eugenol. Adding the three components simultaneously can significantly improve the control effect of the pharmaceutical composition on tobacco fungal diseases.

[0092] The above are only the preferred embodiments of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the principle of the present invention, several improvements and modifications can be made, and these improvements and modifications should also be regarded as the protection scope of the present invention.

Claims

1. A pharmaceutical composition for preventing and treating tobacco fungal diseases, characterized in that: Including the following ingredients: Plant extracts, compound microbial agents, eugenol, emulsifier, thickener, water.

2. The pharmaceutical composition for preventing and controlling tobacco fungal diseases according to claim 1, characterized in that: The invention comprises the following raw materials in parts by weight: 10-15 parts of plant extract components, 2-5 parts of composite microbial agents, 2-5 parts of eugenol, 0.5-0.8 parts of emulsifiers, 0.1-0.5 parts of thickeners, and 60-100 parts of water.

3. The pharmaceutical composition for preventing and controlling tobacco fungal diseases according to claim 1 or 2, characterized in that: The plant extract component comprises the following raw materials in parts by weight: 1-3 parts of peony extract, 2-5 parts of forsythia extract, 1-3 parts of cinnamon extract, and 2-5 parts of angelica dahurica extract.

4. The pharmaceutical composition for preventing and controlling tobacco fungal diseases according to claim 1 or 2, characterized in that: The composite microbial agent comprises the following raw materials in parts by weight: 2-3 parts of Trichoderma harzianum and 3-4 parts of Bacillus velezii.

5. The pharmaceutical composition for preventing and controlling tobacco fungal diseases according to claim 1 or 2, characterized in that: The emulsifier is any one or more of Tween-20, Tween-80 or Tween-60.

6. The pharmaceutical composition for preventing and controlling tobacco fungal diseases according to claim 1 or 2, characterized in that: The thickener is any one or more of xanthan gum, magnesium aluminum silicate and gum arabic.

7. A method for preparing the pharmaceutical composition for preventing and controlling tobacco fungal diseases according to any one of claims 1 to 6, characterized in that: The preparation method comprises the following steps: S1: Preparation of peony extract: crushing dried peony, mixing and soaking with anhydrous ethanol, then extracting under ultrasonic conditions, centrifuging to obtain supernatant, mixing the supernatant with anhydrous ethanol and soaking, reflux extraction, cold storage precipitation, rotary evaporation to remove the solvent, and vacuum freeze drying to obtain peony extract; S2: Preparation of Forsythia suspensa extract: adding dried Forsythia suspensa leaves to water for decoction, filtering out the medicinal liquid, adding water to the medicinal residue for decoction again, filtering out the medicinal liquid, combining the two medicinal liquids, and concentrating; then adding anhydrous ethanol for soaking and refrigerating, filtering, heating the filtrate for extraction by reflux, and then removing the ethanol by rotary evaporation, and drying to obtain the Forsythia suspensa extract; S3: Preparation of cinnamon extract: mixing cinnamon with water and soaking to obtain soaking extract and cinnamon residue; adding ethanol to the cinnamon residue for reflux extraction to obtain reflux extract; combining the soaking extract and the reflux extract, concentrating and drying to obtain cinnamon extract; S4: Preparation of Angelica dahurica extract: crush the dried Angelica dahurica, add ethanol for oscillation extraction, filter, collect the filtrate, then centrifuge the filtrate, collect the supernatant, remove the solvent in the supernatant by rotary evaporation, and dry to obtain the Angelica dahurica extract; S5: mixing the peony extract, the forsythia extract, the cinnamon extract, and the angelica dahurica extract to obtain a plant extract component; S6: mixing Trichoderma harzianum and Bacillus velezii to obtain a composite microbial agent; S7: the plant extract component and the composite microbial agent are added into water to obtain a mixed solution, and then eugenol, an emulsifier and a thickener are added and stirred evenly to obtain the pharmaceutical composition for preventing and controlling tobacco fungal diseases.

8. The method for preparing the pharmaceutical composition for preventing and controlling tobacco fungal diseases according to claim 7, characterized in that: The stirring speed in step S7 is 700-800 r / min, and the stirring time is 5-10 min.

9. Use of the pharmaceutical composition for preventing and controlling tobacco fungal diseases according to any one of claims 1 to 6, characterized in that: The pharmaceutical composition is used for preventing and treating tobacco brown spot disease, black shank disease and black root rot.

10. Use of the pharmaceutical composition for preventing and controlling tobacco fungal diseases according to claim 9, characterized in that: The following steps are involved: The composite bacterial agent and water are prepared into a mixed solution with a concentration of 1.0wt% to 1.5wt%, and the mixed solution is evenly spread on the moist soil surface during the tobacco seedling stage.

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