Preparation method of cannabidiol-loaded grease body and obtained product

Abstract

The invention provides a preparation method of a cannabidiol-loaded grease body and an obtained product, and the preparation method comprises the following operations: dissolving cannabidiol, preparing a grease body dispersion liquid, encapsulating and performing centrifugal separation: after the encapsulating process is completed, performing centrifugal treatment on a reaction mixture, and separating upper-layer paste to obtain the CBD-loaded grease body. According to the preparation method of the oil body loaded with cannabidiol, provided by the invention, the efficient encapsulation of the cannabidiol (CBD) is realized, and the stability and the bioavailability of the CBD are remarkably improved.

Description

Technical Field

[0001] The present invention belongs to the technical field of biological compositions of drugs, and particularly relates to a preparation method of a biological composition loaded with cannabidiol and the resulting product. Background Art

[0002] Cannabidiol (CBD) is a non-psychoactive compound extracted from cannabis plants and has received extensive attention in recent years due to its various potential biological activities. Research shows that CBD has various pharmacological effects such as anti-inflammatory, analgesic, anti-anxiety, anti-depressant, and anti-epileptic, and has been widely used in the fields of medicine and others. However, CBD faces many challenges in practical applications. First, CBD is hydrophobic and difficult to dissolve in a water-based environment, which results in low absorption and bioavailability in the body. In addition, CBD is easily degraded under exposure to air, light, and high temperature conditions, and has poor stability. Therefore, how to improve the solubility, stability, and in vivo delivery efficiency of CBD has become a key issue in the application of CBD.

[0003] To solve these problems, in recent years, researchers have developed a series of nanocarrier technologies, such as liposomes, nanoemulsions, solid lipid nanoparticles, etc. These carriers can encapsulate CBD to improve its water solubility, stability, and achieve a sustained release effect. However, there are certain limitations in the carrier materials or preparation methods adopted by many existing technologies. For example, some carriers require the use of organic solvents or surfactants, which may introduce toxicity or residue problems, limiting their application in the food or drug fields. In addition, the encapsulation efficiency and delivery efficiency of existing carriers are not yet ideal and cannot fully meet the actual needs.

[0004] Oleosomes are lipid structures naturally present in plant seeds, composed of a single layer of phospholipid molecules, interfacial proteins, and neutral fats, and have excellent biocompatibility and natural stability. As a potential new natural plant-based carrier, oleosomes can improve the dispersibility, stability, and bioavailability of hydrophobic active ingredients such as CBD in a water-based environment. Compared with other artificial carriers, oleosomes are natural in origin, do not require the introduction of organic solvents or synthetic chemicals, have high safety and low preparation costs, and conform to the concept of green environmental protection. Therefore, using oleosomes as carriers for CBD has important research value and application prospects. Summary of the Invention

[0005] Aiming at the deficiencies of the existing technology, the purpose of the present invention is to propose a preparation method of oleosomes loaded with cannabidiol to overcome the problems of poor solubility, low stability, and insufficient bioavailability of CBD existing in the prior art.

[0006] The second purpose of the present invention is to propose the product obtained by the above preparation method.

[0007] The technical solution for achieving the above object of the present invention is as follows:

[0008] A preparation method of cannabidiol-loaded oleosomes, comprising the following operations:

[0009] 1) Dissolution of cannabidiol: Cannabidiol is dissolved in absolute ethanol to form a uniform CBD-ethanol solution;

[0010] 2) Preparation of oleosome dispersion: The CBD-ethanol solution is slowly added to the oleosome dispersion, and the oleosome dispersion is formed by dispersing oleosomes in water;

[0011] 3) Encapsulation: The mixture obtained in step 2) is encapsulated at a stirring rate of 50-500 rpm, so that cannabidiol is evenly distributed and captured by oleosomes;

[0012] 4) Centrifugal separation: After the encapsulation process is completed, the reaction mixture is centrifuged to separate the upper paste to obtain cannabidiol-loaded oleosomes.

[0013] Take an appropriate amount of cannabidiol (CBD) and dissolve it in a specific amount of ethanol to form a uniform CBD-ethanol solution. Ethanol, as a co-solvent, can effectively help CBD dissolve and disperse in an aqueous environment, and is easy to separate and volatilize in subsequent processes, facilitating removal without affecting the safety of the product.

[0014] The following are the preferred technical solutions of the present invention.

[0015] Among them, in step 1), the mass ratio of cannabidiol to ethanol is 0.01-1:5-10.

[0016] Among them, in step 2), the mass concentration of oleosomes is 5-15%.

[0017] The mass ratio of lipids in the oleosomes is more than 70%. The oleosomes are oleosomes extracted from hemp seeds. The oleosomes extracted from hemp seeds have the highest compatibility with cannabidiol; the oleosomes extracted from hemp seeds can make the ingredient list cleaner, achieve the purpose of clean label, and have a low environmental cost.

[0018] Furthermore, the oleosomes are oleosomes extracted from hemp seeds, and the mass ratio of lipids to the mass ratio of proteins is 5-8%.

[0019] The oleosome dispersion can provide a stable carrier system to enable effective encapsulation of CBD.

[0020] Among them, in step 1), the mass ratio of cannabidiol to ethanol is 0.01-1:7-8.

[0021] The mixture is encapsulated at a specific stirring rate, encapsulation temperature and encapsulation time to ensure that CBD can be evenly distributed and captured by the grease body. After the encapsulation process is completed, the sample is centrifuged. By centrifugation, the grease body loaded with CBD can be separated from the solution, and the upper paste material is collected to obtain the grease body loaded with CBD.

[0022] Furthermore, in step 3), the encapsulation temperature is 20° C. to 60° C., and the encapsulation time is 8 to 60 minutes.

[0023] Furthermore, in step 3), the stirring rate is 50 to 100 rpm.

[0024] Wherein, in step 2), the CBD-ethanol solution is added once or gradually for multiple times; the mass ratio of the CBD-ethanol solution to the lipid body dispersion is 5-10:90-110.

[0025] Another preferred technical solution of the present invention is that the preparation method comprises the following operations:

[0026] 1) Dissolution of cannabidiol: cannabidiol is dissolved in anhydrous ethanol to form a uniform CBD-ethanol solution;

[0027] 2) preparing a lipid body dispersion: slowly adding the CBD-ethanol solution into the lipid body dispersion at one time, wherein the mass content of lipid bodies in the lipid body dispersion is 9-11%;

[0028] 3) Encapsulation: The mixture obtained in step 2) is encapsulated at a stirring rate of 100 to 120 rpm, the encapsulation time is 9 to 12 minutes, and the encapsulation temperature is 20 to 22° C.;

[0029] 4) Centrifugal separation: After the encapsulation process is completed, the reaction mixture is centrifuged at 5000-8000g for 25-35 minutes at 4-6°C;

[0030] 5) Secondary dispersion and pH adjustment: The CBD-loaded lipid bodies obtained in step 4) are dispersed again in deionized water at a ratio of 1:8-10 (w / w), and the pH is adjusted to 6-8.

[0031] The pH value can be adjusted using NaOH and HCl solutions, or other pH adjusters known in the art.

[0032] The oil body loaded with cannabidiol prepared by the preparation method of the present invention

[0033] The present invention provides a CBD-loaded oleosome and its preparation method. By regulating key parameters such as oleosome concentration, CBD concentration, ethanol concentration, encapsulation temperature, stirring rate, etc., efficient encapsulation of CBD is achieved, greatly improving its encapsulation efficiency and stability, and providing a new solution for the application of CBD.

[0034] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0035] The preparation method of the CBD-loaded oleosome proposed by the present invention realizes the efficient encapsulation of cannabidiol (CBD) and significantly improves the stability and bioavailability of CBD.

[0036] Compared with the prior art, the present invention has the following advantages:

[0037] 1. Environmentally friendly and safe process: The present invention uses natural oleosomes as carriers, avoiding problems such as the use of artificial synthetic components and organic solvent residues, meeting the requirements of green environmental protection, and being particularly suitable for fields such as food and medicine.

[0038] 2. High encapsulation efficiency and good stability: By reasonably selecting encapsulation parameters, the present invention significantly improves the solubility of CBD in the water-based environment, thereby improving its encapsulation efficiency in oleosomes. The prepared CBD-loaded oleosomes show good stability during storage, extending the product shelf life.

[0039] 3. Simple operation and low cost: The preparation method of the present invention is simple and easy to implement, with a low process cost, and has good industrial application prospects.

[0040] 4. Wide application fields: The CBD-loaded oleosomes can be applied in the pharmaceutical field, with broad market prospects and commercial value. BRIEF DESCRIPTION OF THE DRAWINGS

[0041] Figure 1 It is a process flow chart for the preparation of the CBD-loaded oleosomes of the present invention.

[0042] Figure 2 It is a comparison chart of the long-term physical stability of the CBD-loaded oleosomes.

[0043] Figure 3 It is a comparison chart of the CBD retention rate of the CBD-loaded oleosomes. DETAILED DESCRIPTION OF THE INVENTION

[0044] The following examples are used to illustrate the present invention, but are not used to limit the scope of the present invention.

[0045] Unless otherwise specified, the test raw materials and testing instruments used in the specification are commercially available.

[0046] In the examples, high performance liquid chromatography (HPLC) was used to determine the content of CBD in the CBD-loaded liposomes. The encapsulation efficiency of the products obtained in each example was calculated according to the following formula:

[0047]

[0048] The liposomes used were liposomes extracted from hemp seeds, which were 74.9 ± 3.5 wt% lipid and 5.9 ± 0.4 wt% protein by mass ratio, and the balance was 23.2 ± 2.2 wt% water and the like.

[0049] Example 1:

[0050] This example provides a method for preparing CBD-loaded liposomes. The process is shown in Figure 1 .

[0051] It includes the following steps:

[0052] 1) Dissolution of cannabidiol: 0.1 g of CBD was dissolved in 7.5 g of absolute ethanol and stirred at room temperature until completely dissolved to obtain a uniform CBD-ethanol solution. The role of ethanol is not only to dissolve CBD, but also to increase the interaction between liposomes and CBD.

[0053] 2) Preparation of liposome dispersion: 92.4 g of the purified liposome dispersion was taken, and the dispersion contained 5 g of liposomes, and the pH was adjusted to 7. At this time, the liposomes presented stable dispersed droplets, which were suitable for the encapsulation of CBD.

[0054] 3) Encapsulation: The above CBD-ethanol solution was slowly added dropwise to the liposome dispersion at one time, and stirred at a stirring rate of 100 rpm for 10 minutes, and the temperature was set at 20 °C.

[0055] 4) Centrifugal separation: The encapsulated mixture was centrifuged at 5000 g for 30 minutes at 4 °C. The CBD-loaded liposomes were separated, and the upper paste was the liposomes successfully encapsulating CBD.

[0056] 5) Secondary dispersion: The upper paste was dispersed in deionized water at a ratio of 1:9 (w / w) to dilute the liposomes again, and the pH of the solution was adjusted to 7 to obtain the CBD-loaded liposomes.

[0057] The obtained CBD-loaded liposomes were redispersed in deionized water at a ratio of 1:9 (w / w), and the pH was adjusted to 7 for further analysis and determination of encapsulation efficiency. The content of CBD in the CBD-loaded liposomes was determined by high performance liquid chromatography (HPLC) or other appropriate analytical methods, and its encapsulation efficiency was calculated. Through systematic research on influencing factors, the encapsulation conditions were optimized to ensure the best encapsulation efficiency.

[0058] Examples 2 to 3

[0059] This example provides a CBD-loaded oleosome, which uses a preparation method substantially the same as that of Example 1. The difference from Example 1 is only that the mass of the oleosome in step 2) is different, which are 10 g and 15 g of the addition amounts respectively.

[0060] Test Example 1

[0061] Compare the CBD-loaded oleosomes of Examples 1 to 3;

[0062] Effect of the addition amount of the oleosome in step 2) on the average particle size and Zeta potential of the CBD-loaded oleosome: As can be seen from the following table, the average particle size and Zeta potential of the CBD-loaded oleosome do not increase with the increase of the addition amount of the oleosome and remain relatively stable.

[0063] Effect of the addition amount of the oleosome on the encapsulation efficiency of the CBD-loaded oleosome: As can be seen from the following table, when the addition amount of the oleosome increases from 5 g to 10 g, the encapsulation efficiency of CBD increases from 75% to 86%. However, when the addition amount of the oleosome increases from 10 g to 15 g, the encapsulation efficiency of CBD in the oleosome decreases to 74%, indicating that the encapsulation efficiency obtained when the addition amount of the oleosome is 10 g is the highest. Therefore, the addition amount of the oleosome is selected as 10 g as the best.

[0064] Table 1. Effect of the addition amount of different oleosomes on the average particle size, Zeta potential and encapsulation efficiency of the CBD-loaded oleosome

[0065] Addition amount of fat body (g) Average particle size (μm) Zeta potential (mV) Encapsulation rate (%) 5 2.2±0.1 -31.5±4.7 75.2±4.6 10 2.3±0.2 -34.4±5.3 86.3±6.7 15 2.3±0.2 -33.5±4.8 74.4±3.8

[0066] Examples 4 to 5

[0067] This example provides a CBD-loaded oleosome, which uses a preparation method substantially the same as that of Example 2. The difference from Example 2 is that the mass of CBD in step 1) is different, which are 0.01 g and 1.0 g of the addition amounts respectively. The addition amount of the oleosome in step 2) is 10 g.

[0068] Test Example 2

[0069] Compare the CBD-loaded oleosomes of Example 2 and Examples 4 to 5;

[0070] Effect of the addition amount of CBD in step 1) on the average particle size and Zeta potential of the CBD-loaded oleosome: As can be seen from the following table, the average particle size and Zeta potential of the CBD-loaded oleosome do not increase with the increase of the addition amount of CBD and remain relatively stable.

[0071] Effect of the addition amount of CBD on the encapsulation efficiency of CBD-loaded oil bodies: As can be seen from the following table, with the increase of the addition amount of CBD, the encapsulation efficiency of CBD in the oil bodies decreases accordingly. When the addition amount of CBD is 1.0 g, the encapsulation efficiency of CBD drops to 64%, indicating that when the content of CBD in the system is too high, more CBD cannot enter the oil bodies and thus reaches a saturation state. Considering that as much CBD as possible should be loaded in the oil bodies, 0.1 g of CBD is selected as the optimal addition amount.

[0072] Table 2. Effects of different addition amounts of CBD on the average particle size, Zeta potential and encapsulation efficiency of CBD-loaded oil bodies

[0073] Amount of CBD added (g) Average particle size (μm) Zeta potential (mV) Encapsulation rate (%) 0.01 2.3±0.1 -34.5±3.7 91.1±3.8 0.1 2.2±0.2 -33.4±4.3 86.2±6.1 1.0 2.3±0.1 -33.8±4.2 64.4±6.9

[0074] Examples 6 - 7

[0075] This example provides a CBD-loaded oil body, using a preparation method basically the same as that in Example 2. The difference from Example 2 is only the mass of ethanol in step 1), which is 5 g and 10 g of addition amounts respectively.

[0076] Test Example 3

[0077] Compare the CBD-loaded oil bodies of Example 2 and Examples 6 - 7;

[0078] Effect of the addition amount of ethanol as a co-solvent on the average particle size and Zeta potential of CBD-loaded oil bodies: As can be seen from the following table, the average particle size and Zeta potential of CBD-loaded oil bodies do not increase with the increase of the addition amount of ethanol and remain relatively stable.

[0079] Effect of the addition amount of ethanol in step 1) on the encapsulation efficiency of CBD-loaded oil bodies: As can be seen from the following table, with the increase of the addition amount of ethanol, the encapsulation efficiency of CBD in the oil bodies first increases and then decreases. When the addition amount of ethanol is 7.5 g, the encapsulation efficiency of CBD reaches the highest at 86%. This is because in the case of low ethanol addition amounts, CBD cannot be fully dissolved and dispersed in the system, so some CBD will precipitate or cannot be successfully encapsulated into the oil bodies; in the case of high ethanol addition amounts, the content of ethanol is too high, which will affect the interfacial properties of the oil bodies, and at the same time, too much ethanol will affect the interaction between CBD and the oil bodies, so the encapsulation efficiency of CBD will decrease. Therefore, choosing 7.5 g of ethanol as the co-solvent will obtain the highest CBD encapsulation efficiency and maintain the stability of the oil bodies.

[0080] Table 3. Effects of different addition amounts of ethanol on the average particle size, Zeta potential and encapsulation efficiency of CBD-loaded oil bodies

[0081] Amount of ethanol added (g) Average particle size (μm) Zeta potential (mV) Encapsulation rate (%) 5 2.3±0.1 -32.8±5.2 76.2±4.6 7.5 2.3±0.2 -33.4±6.2 86.3±5.7 10 2.4±0.2 -35.5±4.9 81.4±3.8

[0082] Examples 8 - 9

[0083] This example provides an oil body loaded with CBD. The preparation method is basically the same as that of Example 2, and the difference from Example 2 is only that the encapsulation time in step 3) is different, which is 30 minutes and 60 minutes respectively.

[0084] Test Example 4

[0085] Compare the oil bodies loaded with CBD in Example 2 and Examples 8 - 9;

[0086] Effect of the encapsulation time in step 3) on the average particle size and Zeta potential of the oil body loaded with CBD: As can be seen from the following table, the average particle size and Zeta potential of the oil body loaded with CBD do not increase with the increase of the encapsulation time and remain relatively stable.

[0087] Effect of the encapsulation time on the encapsulation efficiency of the oil body loaded with CBD: As can be seen from the following table, with the increase of the encapsulation time, the encapsulation efficiency of CBD in the oil body remains unchanged and stable. This is because ethanol is used as a co - solvent, and CBD can be fully dispersed in the system, and the whole encapsulation process is completed quickly. Prolonging the encapsulation time has no significant effect on the encapsulation of CBD. Therefore, 10 minutes is selected as the optimal encapsulation time.

[0088] Table 4. Effects of different encapsulation times on the average particle size, Zeta potential and encapsulation efficiency of the oil body loaded with CBD

[0089] Encapsulation time (minutes) Average particle size (μm) Zeta potential (mV) Encapsulation rate (%) 10 2.3±0.2 -32.7±3.9 86.1±4.6 30 2.3±0.2 -33.3±5.8 86.4±6.5 60 2.3±0.2 -35.1±3.9 83.4±4.8

[0090] Examples 10 - 11

[0091] This example provides an oil body loaded with CBD. The preparation method is the same as that of Example 1, and the difference from Example 1 is only that the encapsulation temperature in step 3) is different, which is 40°C and 60°C respectively.

[0092] Test Example 5

[0093] Compare the oil bodies loaded with CBD in Example 1 and Examples 10 - 11;

[0094] Effect of the encapsulation temperature in step 3) on the average particle size and Zeta potential of the oil body loaded with CBD: As can be seen from the following table, the average particle size and Zeta potential of the oil body loaded with CBD do not increase with the increase of the encapsulation temperature and remain relatively stable.

[0095] Effect of encapsulation temperature on the encapsulation efficiency of CBD-loaded liposomes: As can be seen from the following table, with the increase of encapsulation temperature, there is no significant difference in the encapsulation efficiency of CBD in liposomes. Although the increase in temperature will change the interaction between CBD and liposomes, because ethanol is used as a co-solvent, CBD is quickly captured by liposomes and is not affected by temperature instead. Therefore, 20 °C is selected as the optimal encapsulation temperature.

[0096] Table 5. Effects of different encapsulation temperatures on the average particle size, Zeta potential and encapsulation efficiency of CBD-loaded liposomes

[0097] Encapsulation temperature (℃) Average particle size (μm) Zeta potential (mV) Encapsulation rate (%) 20 2.3±0.3 -34.2±4.2 86.4±3.6 40 2.2±0.2 -34.3±5.3 85.6±6.8 60 2.3±0.1 -34.5±6.3 87.4±5.1

[0098] Examples 12 - 13

[0099] This example provides a CBD-loaded liposome, using a preparation method basically the same as that of Example 2. The difference from Example 2 is only that the stirring rates in step 3) are different, which are 300 rpm and 500 rpm respectively.

[0100] Test Example 6

[0101] Compare the CBD-loaded liposomes of Example 1 and Examples 12 - 13;

[0102] Effect of stirring rate on the average particle size and Zeta potential of CBD-loaded liposomes: As can be seen from the following table, the average particle size and Zeta potential of CBD-loaded liposomes do not increase with the increase of stirring rate and remain relatively stable.

[0103] Effect of stirring rate on the encapsulation efficiency of CBD-loaded liposomes: As can be seen from the following table, with the increase of stirring rate, there is no significant difference in the encapsulation efficiency of CBD in liposomes. Therefore, 100 rpm is selected as the optimal stirring rate.

[0104] Table 6. Effects of different stirring rates on the average particle size, Zeta potential and encapsulation efficiency of CBD-loaded liposomes

[0105] Stirring speed (rpm) Average particle size (μm) Zeta potential (mV) Encapsulation rate (%) 100 2.3±0.1 -31.6±3.2 86.3±4.2 300 2.2±0.3 -33.3±4.1 85.8±4.8 500 2.3±0.2 -33.5±4.3 84.5±5.7

[0106] Example 14

[0107] This example provides a CBD-loaded liposome, using the same preparation method as Example 2. The difference from Example 2 is only that the CBD-ethanol solution is added to the liposome dispersion step by step in multiple times at the same time interval during the encapsulation process.

[0108] Test Example 7

[0109] Compare the CBD-loaded liposomes of Example 1 and Example 14;

[0110] The addition method of the CBD-ethanol solution has no significant effect on the average particle size, Zeta potential and encapsulation efficiency of the CBD-loaded liposomes, and the liposomes remain relatively stable. Therefore, considering the simplicity of operation, the addition method of one-time addition was selected.

[0111] Table 7. Effects of different addition methods of CBD-ethanol solution on the average particle size, Zeta potential and encapsulation efficiency of CBD-loaded liposomes

[0112] Add method Average particle size (μm) Zeta potential (mV) Encapsulation rate (%) One-time Join 2.3±0.1 -32.2±3.2 86.3±3.9 Multiple gradual additions 2.3±0.2 -34.6±4.5 86.1±4.7

[0113] Example 15

[0114] This example provides a kind of CBD-loaded liposome, which is prepared by the same preparation method as in Example 2. The obtained sample is stored at 4°C in sealed and light-proof conditions. The average particle size and CBD retention rate of the sample are measured on the 0th, 1st, 3rd, 5th and 7th days respectively. The CBD retention rate is calculated according to the following formula:

[0115]

[0116] Test Example 8

[0117] The average particle size and CBD retention rate of the sample in Example 2 are measured at different storage time periods respectively. From Figure 2 and Figure 3 it can be seen that under the condition of 4°C, the sample still maintains good physicochemical stability after 7 days of storage. There is no significant difference in the average particle size of the sample, and at the same time, the retention rate of CBD is also above 95%. It shows that the liposomes have good long-term physical stability and can effectively prevent the decrease of the effective content caused by the oxidation of the encapsulated CBD and other situations.

[0118] By using natural liposomes as carriers, the present invention provides a green, environmentally friendly and efficient CBD encapsulation technology, which can effectively improve the stability, solubility and bioavailability of CBD, and is applicable to the fields of medicine, food, cosmetics, etc.

[0119] Although the present invention has been described above through examples, those skilled in the art should understand that without departing from the spirit and essence of the present invention, the improvements and modifications made to the present invention shall fall within the protection scope of the present invention.

Claims

1. A method for preparing a cannabidiol-loaded grease body, characterized in that: The following operations are included: 1) Dissolution of cannabidiol: cannabidiol is dissolved in anhydrous ethanol to form a uniform CBD-ethanol solution; 2) preparing a lipid body dispersion: slowly adding the CBD-ethanol solution into a lipid body dispersion, wherein the lipid body dispersion is formed by dispersing lipid bodies in water; 3) Encapsulation: The mixture obtained in step 2) is encapsulated at a stirring rate of 50 to 500 rpm so that the cannabidiol solution is evenly distributed and captured by the lipid body; 4) Centrifugal separation: After the encapsulation process is completed, the reaction mixture is centrifuged to separate the upper paste to obtain the CBD-loaded lipid body.

2. The method for preparing a cannabidiol-loaded grease body according to claim 1, characterized in that: In step 1), the mass ratio of cannabidiol to ethanol is 0.01-1:5-10.

3. The method for preparing a cannabidiol-loaded grease body according to claim 1, characterized in that: The mass concentration of the grease bodies in step 2) is 5-15%; the mass proportion of lipids in the grease bodies is more than 70%.

4. The method for preparing a cannabidiol-loaded grease body according to claim 3, characterized in that: The oil body is extracted from hemp seeds, the mass proportion of lipids is 70-80%, and the mass proportion of proteins is 4-8%.

5. The method for preparing a cannabidiol-loaded grease body according to claim 1, characterized in that: In step 1), the mass ratio of cannabidiol to ethanol is 0.01-1:7-8.

6. The method for preparing a cannabidiol-loaded grease body according to claim 1, characterized in that: In step 3), the encapsulation temperature is 20° C. to 60° C., and the encapsulation time is 8 to 60 minutes.

7. The method for preparing a cannabidiol-loaded grease body according to claim 1, characterized in that: In step 3), the stirring rate is 50-100 rpm.

8. The method for preparing a cannabidiol-loaded grease body according to claim 1, characterized in that: In step 2), the CBD-ethanol solution is added once or gradually over several times; the mass ratio of the CBD-ethanol solution to the lipid body dispersion is 5-10:90-110.

9. The method for preparing a cannabidiol-loaded grease body according to any one of claims 1 to 8, characterized in that: The following operations are included: 1) Dissolution of cannabidiol: cannabidiol is dissolved in anhydrous ethanol to form a uniform CBD-ethanol solution; 2) preparing a lipid body dispersion: slowly adding the CBD-ethanol solution into the lipid body dispersion at one time, wherein the mass content of lipid bodies in the lipid body dispersion is 9-11%; 3) Encapsulation: The mixture obtained in step 2) is encapsulated at a stirring rate of 100 to 120 rpm, the encapsulation time is 9 to 12 minutes, and the encapsulation temperature is 20 to 22° C.; 4) Centrifugal separation: After the encapsulation process is completed, the reaction mixture is centrifuged at 5000-8000g for 25-35 minutes at 4-6°C; 5) Secondary dispersion and pH adjustment: The cannabidiol-loaded oil body obtained in step 4) is dispersed again in deionized water at a ratio of 1:8-10 (w / w), and the pH is adjusted to 6-8.

10. The cannabidiol-loaded lipid body prepared by the preparation method according to any one of claims 1 to 9.

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