Composition for preventing and relieving acute drunkenness

Abstract

The invention relates to a composition for preventing and relieving acute drunkenness. The composition is prepared from the following raw materials in parts by weight: 30 parts of flos puerariae, 10 parts of rhizoma cimicifugae, 10 parts of sedum sarmentosum, 15 parts of rhizoma alismatis, 15 parts of bighead atractylodes rhizome, 30 parts of poria cocos, 15 parts of hairyvein agrimony, 15 parts of galangal and 30 parts of lalang grass rhizome. The composition provided by the invention is a multi-target and multi-path protection process for treating acute alcoholism, and relates to anti-inflammation, anti-allergy, promotion of protein synthesis, immunosuppression, anti-shock, regulation of cholesterol of blood fat, and participation in alcohol decomposition and metabolic pathways; the traditional Chinese medicine composition has the advantages that the traditional Chinese medicine composition can be used for preventing acute drunkenness and reducing alcohol metabolism hazards, including hyperlipidemia, dementia, hypercholesterolemia, fatty liver, cognitive impairment, cardiovascular and cerebrovascular diseases and the like, after drunkenness can be reduced, in addition, the traditional Chinese medicine composition can also protect cardiovascular, nerves and bones after drunkenness, regulate immunity and enhance immunity after drunkenness can also be realized, and the traditional Chinese medicine composition can also be used for preventing drunkenness and preventing drunkenness from suffering from the influence on transcription factors such as SP1, ESR1, RELA (P65), NF-kappa b1, STAT3 and the like.

Description

Technical Field

[0001] The invention relates to the technical field of medicines, and in particular to a composition for preventing and relieving acute drunkenness. Background Art

[0002] Acute alcohol intoxication (AAI), commonly known as drunkenness, has become one of the most common types of poisoning in the emergency department.

[0003] Modern medicine mainly treats AAI with symptomatic supportive treatment, including rehydration, maintaining airway patency, maintaining circulatory function, and maintaining water, electrolyte and acid-base balance. In addition, drugs such as alcohol metabolism promoters, wakefulness promoters and sedatives are also used for intervention. For example, modern medicine often uses metadoxine to promote the metabolism of ethanol in the body for AAI, and cooperates with naloxone hydrochloride to alleviate the damage of ethanol to the central nervous system, but it has no clear effect on gastrointestinal, heart, brain, kidney and other injuries caused by AAI; proton pump inhibitors represented by omeprazole can only reduce the stimulation of gastric acid secretion on the gastrointestinal tract, and have more adverse reactions. However, studies have shown that metadoxine has potential embryo teratogenicity and can cause reproductive toxicity. The safety of frequent or long-term use of metadoxine for alcohol sobering needs further verification. Naloxone has a strong awakening effect and can relieve the patient's clinical symptoms in a short period of time. However, studies have shown that adverse reactions are mainly cardiovascular reactions (such as arrhythmias, hypertension, etc.). Once naloxone is discontinued, it can cause drowsiness in patients with alcohol poisoning. Some patients may also experience adverse reactions such as abnormal emotions and abnormal blood pressure. Omeprazole is metabolized mainly by the liver, so it is more likely to have liver toxicity; its side effects also include common gastrointestinal infections, arrhythmias, insomnia, drowsiness, tinnitus, headaches and dizziness, mental disorders, rashes, blood system damage, and endocrine and reproductive system damage. Because factors such as the patient's age, gender, liver function status and genetic background can affect the efficiency of alcohol metabolism, different patients have different responses to the same treatment plan. For example, some patients may experience more severe poisoning symptoms due to acetaldehyde dehydrogenase gene defects, which makes it difficult for standardized treatment to meet personalized needs. Moreover, even after treatment, many patients will still have symptoms of nervous system and digestive system discomfort such as dizziness, headache, nausea and vomiting. These problems not only affect the quality of the patient's recovery, but may also further increase the burden on the liver and delay the overall recovery process. Modern medicine lacks effective means to prevent acute alcohol poisoning.

[0004] In recent years, a number of clinical studies and animal experiments have shown that traditional Chinese medicine has demonstrated unique advantages in the prevention and treatment of AAI. For example, CN104147173A discloses a traditional Chinese medicine composition for treating acute alcoholism, which is made from the following raw materials in parts by weight: 5 - 15 parts of rhubarb, 10 - 15 parts of kudzu root, 10 - 15 parts of hawthorn, 5 - 15 parts of capillaris, 3 - 10 parts of bupleurum, 10 - 30 parts of hyacinth bean, 5 - 15 parts of polygala, and 10 - 15 parts of wild jujube seed. This composition has the functions of detoxifying, removing toxins, strengthening the spleen and soothing the liver, and nourishing the heart and calming the mind, and can effectively relieve acute alcoholism and the continuous damage effect of residual alcohol on the body. CN105362384A discloses a traditional Chinese medicine composition for relieving alcohol intoxication and its application. This traditional Chinese medicine composition is made from the following raw materials in parts by weight: 3 - 7 parts of raisin tree seed, 1 - 4 parts of flower of kudzu vine, 0.5 - 2 parts of flower of magnolia officinalis, and 1 - 5 parts of liquorice root. The treatment results show that after drunk patients take the traditional Chinese medicine, most patients' symptoms such as dizziness, nausea and vomiting, thirst, abdominal distension and fullness, restlessness and fever after drinking are significantly improved within 1 hour, and the above symptoms basically disappear within 2 hours, and the total effective rate is 100%. Traditional supportive therapies mostly intervene after intoxication occurs, and cannot fundamentally reduce alcohol absorption or accelerate its metabolism, thus it is difficult to effectively prevent and relieve drunkenness.

[0005] In addition, on the one hand, there are differences in the understanding of those skilled in the art; on the other hand, although the applicant has studied a large number of documents and patents when making this invention, due to space limitations, not all details and content are listed in detail. However, this does not mean that this invention does not possess the features of these prior arts. On the contrary, this invention already possesses all the features of the prior arts, and the applicant reserves the right to add relevant prior arts in the background art. Summary of the Invention

[0006] After ethanol enters the human body, it is mainly absorbed through the stomach (about 20%-30%) and the intestines (about 70%-80%). After ingesting ethanol, it usually enters the bloodstream within 5 to 10 minutes and reaches the peak concentration within 20 to 60 minutes. Ethanol is distributed in all water-containing tissues and body fluids in the body, including the brain and alveoli. Among them, about 90% of ethanol is metabolized in the liver: first, it is converted into acetaldehyde under the action of alcohol dehydrogenase, and then acetaldehyde is further metabolized into acetic acid under the catalysis of acetaldehyde dehydrogenase. Acetic acid can be completely decomposed into carbon dioxide and water in the liver, or it can participate in the energy metabolism of the body through other metabolic pathways. The carbon dioxide produced by metabolism is mainly excreted from the body through pulmonary respiration. In addition, about 10% of ethanol is excreted directly through the kidneys with urine without metabolism, and ethanol and its metabolites may also be excreted through sweat and other channels. Therefore, the key measures for preventing and treating acute alcohol poisoning include: protecting the gastrointestinal barrier to delay alcohol absorption; protecting liver function and increasing the activity of acetaldehyde dehydrogenase, thereby enhancing the metabolism and detoxification ability of alcohol; strengthening diuresis to promote alcohol excretion; and minimizing the adverse reactions that drugs may cause. These measures help to effectively reduce the toxic effects of alcohol on the body.

[0007] In view of the deficiencies of the prior art, the first aspect of the present invention provides a composition for preventing and alleviating acute drunkenness, which is prepared from the following raw materials in parts by weight: 30 parts of Puerariae Flos, 10 parts of Cimicifugae Rhizoma, 10 parts of Sedum sarmentosum Bunge, 15 parts of Alismatis Rhizoma, 15 parts of Atractylodis Macrocephalae Rhizoma, 30 parts of Poria, 15 parts of Agrimonia pilosa Ledeb., 15 parts of Galangae Rhizoma, 30 parts of Imperatae Rhizoma.

[0008] According to a preferred embodiment, the Atractylodis Macrocephalae Rhizoma is stir-fried Atractylodis Macrocephalae Rhizoma and / or roasted Atractylodis Macrocephalae Rhizoma.

[0009] The second aspect of the present invention provides a preparation method of a composition for preventing and alleviating acute drunkenness, comprising the following steps:

[0010] Except for Atractylodis Macrocephalae Rhizoma, soak the raw material herbs in formula amounts separately with clear water and then decoct, collect the medicinal liquid and cool it, and then add the Atractylodis Macrocephalae Rhizoma enzymatic hydrolysate to obtain the composition.

[0011] According to a preferred embodiment, the amount of clear water used is 1.5 times that of the raw material herbs.

[0012] According to a preferred embodiment, the soaking time is 2 hours.

[0013] According to a preferred embodiment, the decocting duration is 30 minutes.

[0014] According to a preferred embodiment, collect the medicinal liquid and cool it to 40 - 50 °C before adding the Atractylodis Macrocephalae Rhizoma enzymatic hydrolysate.

[0015] According to a preferred embodiment, the Atractylodes macrocephala enzyme hydrolysate includes a first Atractylodes macrocephala enzyme hydrolysate prepared from stir-fried Atractylodes macrocephala and a second Atractylodes macrocephala enzyme hydrolysate prepared from honey-fried Atractylodes macrocephala.

[0016] According to a preferred embodiment, the first Atractylodes macrocephala enzyme hydrolysate is prepared by the following steps:

[0017] After stir-frying Atractylodes macrocephala, it is added to soaking water for decoction to obtain the first Atractylodes macrocephala liquid;

[0018] The first Atractylodes macrocephala liquid after decoction is cooled down;

[0019] Cellulase, protease, and amylase with a mass ratio of 1.5:1:2 are added for enzymatic hydrolysis;

[0020] By reflux extraction and purification, the first Atractylodes macrocephala enzyme hydrolysate is prepared.

[0021] According to a preferred embodiment, the second Atractylodes macrocephala enzyme hydrolysate is prepared by the following steps:

[0022] After honey-frying Atractylodes macrocephala, it is added to soaking water for decoction to obtain the second Atractylodes macrocephala liquid;

[0023] The second Atractylodes macrocephala liquid after decoction is cooled down;

[0024] Cellulase, protease, and amylase with a mass ratio of 1.5:1:2 are added for enzymatic hydrolysis;

[0025] By reflux extraction and purification, the second Atractylodes macrocephala enzyme hydrolysate is prepared.

[0026] According to a preferred embodiment, in the step of preparing the first Atractylodes macrocephala liquid or the second Atractylodes macrocephala liquid, the decoction temperature is 70°C.

[0027] According to a preferred embodiment, in the step of preparing the first Atractylodes macrocephala liquid or the second Atractylodes macrocephala liquid, the decoction duration is 15 minutes.

[0028] According to a preferred embodiment, the first Atractylodes macrocephala liquid and the second Atractylodes macrocephala liquid after decoction are respectively cooled to 40 - 50°C before enzymatic hydrolysis.

[0029] The third aspect of the present invention provides the application of the composition provided by the first aspect of the present invention and the preparation method of the composition provided by the second aspect of the present invention in the preparation of substances for prolonging the drunkenness latency period and / or shortening the hangover time. Preferably, the substances for prolonging the drunkenness latency period and / or shortening the hangover time can be health products, foods, or drugs.

[0030] The fourth aspect of the present invention provides the application of the composition provided by the first aspect of the present invention and the preparation method of the composition provided by the second aspect of the present invention in the preparation of substances for treating inflammatory diseases. Preferably, the substances for treating inflammatory diseases can be health products, foods, or drugs.

[0031] The fifth aspect of the present invention provides the use of the composition provided in the first aspect of the present invention and the preparation method of the composition provided in the second aspect of the present invention in reducing the release level of inflammatory factors.

[0032] According to a preferred embodiment, the inflammatory factors include one or more of interleukin-6, tumor necrosis factor-α, and interleukin-10.

[0033] The sixth aspect of the present invention provides the use of the composition provided in the first aspect of the present invention and the preparation method of the composition provided in the second aspect of the present invention in inhibiting the activation of the signal pathway of transcription activator 3.

[0034] The seventh aspect of the present invention provides the use of the composition provided in the first aspect of the present invention and the preparation method of the composition provided in the second aspect of the present invention in the preparation of a substance for treating liver injury. Preferably, the substance for treating liver injury can be a health product, food, or drug.

[0035] The eighth aspect of the present invention provides the use of the composition provided in the first aspect of the present invention and the preparation method of the composition provided in the second aspect of the present invention in the preparation of a substance for treating gastric mucosal injury. Preferably, the substance for treating gastric mucosal injury can be a health product, food, or drug. Description of the Drawings

[0036] Figure 1 It is a Venn diagram of 19 possible action targets for the traditional Chinese medicine formula for relieving hangover provided by the present invention to treat acute alcoholism;

[0037] Figure 2 It is the result of gene ontology analysis provided by the present invention;

[0038] Figure 3 It is the display result of the MCODE components extracted through the protein interaction network provided by the present invention;

[0039] Figure 4 It is the enrichment analysis result in DisGeNET provided by the present invention;

[0040] Figure 5 It is the enrichment analysis result in PaGeneBase provided by the present invention;

[0041] Figure 6 It is the enrichment analysis result of TRRUST provided by the present invention;

[0042] Figure 7 It is the statistical result of the activity ability of mice over time provided by the present invention;

[0043] Figure 8 It is the observation result of the behavioral state of mice provided by the present invention;

[0044] Figure 9 Statistical results of mouse blood biochemical indexes provided by the present invention;

[0045] Figure 10 Statistical results of mouse blood biochemical indexes in the 15-day acute toxicity experiment provided by the present invention;

[0046] Figure 11 Release levels of IL-6 in the sera of mice in each group provided by the present invention;

[0047] Figure 12 Release levels of TNF-α in the sera of mice in each group provided by the present invention;

[0048] Figure 13 Release levels of IL-10 in the sera of mice in each group provided by the present invention;

[0049] Figure 14 Expression conditions of STAT3 protein detected by immunoblotting in liver tissues of mice in each group provided by the present invention;

[0050] Figure 15 Pathological scores of liver tissues of mice in each group provided by the present invention;

[0051] Figure 16 Pathological tissue sections of liver tissues of mice in each group provided by the present invention;

[0052] Figure 17 Pathological scores of gastric mucosal injuries of mice in each group provided by the present invention;

[0053] Figure 18 Pathological sections of gastric mucosa of mice in each group provided by the present invention. Detailed implementation manners

[0054] The present invention will be described in detail below with reference to the accompanying drawings and specific embodiments. It should be understood that these descriptions are exemplary and are not intended to limit the scope of the present disclosure. In the following detailed description, for the sake of explanation, many specific details are set forth to provide a comprehensive understanding of the embodiments of the present disclosure. However, obviously, one or more embodiments can also be implemented without these specific details. In addition, in the following description, descriptions of well-known structures and technologies are omitted to avoid unnecessarily confusing the concepts of the present disclosure.

[0055] All terms used herein (including technical and scientific terms) have the meanings commonly understood by those skilled in the art, unless otherwise defined. It should be noted that the terms used herein should be interpreted as having a meaning consistent with the context of this specification and should not be interpreted in an idealized or overly rigid manner.

[0056] In the following embodiments, various processes and methods not described in detail are conventional methods or technical means in the art.

[0057] The pharmacology of the present invention is as follows:

[0058] Flos Puerariae: This product is sweet, pungent, and cool in nature, and belongs to the spleen and stomach meridians. It is mainly used to treat fever and thirst due to excessive drinking, headache and dizziness, fullness and distension in the epigastric and abdominal regions, vomiting and acid regurgitation, loss of appetite, hematemesis, and intestinal wind with bloody stools.

[0059] Rhizoma Cimicifugae: This product is pungent, slightly sweet, and slightly cold in nature, and belongs to the lung, spleen, stomach, and large intestine meridians. Rhizoma Cimicifugae has the effects of inducing sweating and rash, clearing heat and detoxifying, and lifting yang qi. In addition, Rhizoma Cimicifugae and its active ingredients can inhibit the inflammatory cytokine storm induced by acute-on-chronic liver failure and reduce liver necrosis.

[0060] Herba Sedi Sarmentosi: This product is sweet, light, and cool in nature, and belongs to the liver, gallbladder, and small intestine meridians. Its main effects are promoting diuresis and reducing jaundice, clearing heat and detoxifying, and promoting blood circulation to remove stasis.

[0061] Rhizoma Alismatis: This product is sweet, light, and cold in nature, and belongs to the kidney and bladder meridians. It has the effects of promoting diuresis and percolating dampness, inducing diuresis and clearing heat, and reducing turbidity and lipid-lowering. Rhizoma Alismatis is cold in nature and can clear away heat toxins in the body and improve symptoms of febrile diseases, such as thirst, yellow urine, dizziness, vertigo, soreness and weakness of the waist and knees, etc. It can reduce the total cholesterol and blood lipids in the blood and has a good therapeutic effect on hyperlipidemia caused by alcohol. At the same time, it also has the effect of antiplatelet aggregation, which helps to prevent cardiovascular diseases induced by inflammation after drinking. In addition, it also has certain anti-inflammatory and antibacterial effects and can assist in the treatment of infections after alcohol-induced damage to the gastrointestinal mucosa.

[0062] Rhizoma Atractylodis Macrocephalae: This product is bitter, sweet, and warm in nature, and belongs to the spleen and stomach meridians. Rhizoma Atractylodis Macrocephalae can strengthen the spleen and replenish qi, enhance the functions of the spleen and stomach, and promote digestion; dry dampness and promote diuresis, which helps to improve damp-heat alcoholism and promote the excretion of alcohol from urine; replenish middle qi and relieve fatigue and weakness after drinking. Rhizoma Atractylodis Macrocephalae has two preparation methods: honey-fried and stir-fried. The similarities are that both retain the basic effects of strengthening the spleen and replenishing qi, and drying dampness and promoting diuresis. The differences are as follows:

[0063] Rhizoma Atractylodis Macrocephalae Praeparata cum Melle: It is stir-fried with honey. Honey is sweet and slow-acting, and has the effects of tonifying the middle and moistening dryness, relieving pain and detoxifying. After being stir-fried with honey, Rhizoma Atractylodis Macrocephalae Praeparata cum Melle enhances the effect of invigorating the spleen and replenishing qi, and is mainly used to treat weakness of the spleen and stomach, deficiency of middle qi, such as lassitude, loss of appetite, abdominal distension and loose stools, etc., and is more suitable for those with deficiency-cold of the spleen and stomach.

[0064] Rhizoma Atractylodis Macrocephalae Praeparata: After being stir-fried with yellow soil or wheat bran, the effect of Rhizoma Atractylodis Macrocephalae in strengthening the spleen and drying dampness is enhanced, and it is commonly used in the syndrome of spleen deficiency with excessive dampness and internal retention of water-dampness, such as edema and phlegm-fluid retention caused by spleen deficiency; and after stir-frying, its warm and dry nature is relatively mild, and it can also be applied to those with excessive dampness and a tendency to transform into heat.

[0065] Poria cocos: This herb is sweet, bland, and neutral in nature, and it pertains to the Heart, Lung, Spleen, and Kidney meridians. It has the effects of promoting diuresis to eliminate dampness, strengthening the spleen and stomach, and calming the mind. Poria cocos polysaccharide has the function of protecting the gastrointestinal mucosa, regulating the spleen and stomach, relieving vomiting, anorexia, indigestion, etc. after drinking; promoting water metabolism and promoting the excretion of alcohol through urine; it can also relieve restlessness, insomnia, etc., and helps to improve the over-inhibited or over-active state of the cerebral cortex after drinking and enhance the quality of normal sleep. In addition, it also has the effects of enhancing immune function and lowering blood sugar.

[0066] Agrimony: This herb is bitter, astringent, and neutral in nature, and it pertains to the Lung, Heart, Liver, Spleen, and Large Intestine meridians. Its therapeutic effects mainly include: arresting bleeding with astringents, stopping dysentery and sterilizing, tonifying deficiency and strengthening the body, anti-inflammatory and detoxifying, lowering blood pressure, etc. It can be used for gastrointestinal mucosal bleeding caused by alcohol stimulation, diarrhea and dysentery caused by bacteria, and relieve symptoms such as physical weakness after drinking. It also has the effect of vasodilation, which helps to relieve the symptom of elevated blood pressure after drinking.

[0067] Galangal: This herb is pungent in taste and hot in nature, and it pertains to the Spleen, Stomach, and Lung meridians. Its main effects are warming the middle-jiao to arrest vomiting, relieving pain, etc. Galangal can harmonize the stomach and arrest vomiting, relieve stomachache, indigestion, etc. caused by alcohol, inhibit ulcer formation, and improve gastrointestinal function.

[0068] Rhizoma Imperatae: It is sweet in taste and cold in nature. It pertains to the Lung, Stomach, and Bladder meridians, and has the effects of clearing heat and detoxifying, promoting diuresis and relieving stranguria, cooling blood to stop bleeding, reducing fire and moistening the lungs, etc. It can effectively relieve symptoms such as fever, dry mouth and throat pain caused by damp-heat of alcohol toxin, promote the generation and excretion of urine; reduce gastrointestinal mucosal bleeding, inflammatory reaction and pain caused by alcohol. In addition, Rhizoma Imperatae also has the effects of enhancing immunity, antibacterial, lowering blood lipid, blood sugar, and blood pressure.

[0069] Example 1

[0070] This example provides a composition, especially a composition for preventing and relieving acute drunkenness. This composition is prepared from the following raw materials in parts by weight: 30 parts of Puerariae flos, 10 parts of Cimicifugae rhizoma, 10 parts of Sedum sarmentosum Bunge, 15 parts of Alismatis rhizoma, 15 parts of Atractylodis macrocephalae rhizoma, 30 parts of Poria cocos, 15 parts of Agrimony, 15 parts of Galangal, 30 parts of Rhizoma Imperatae. In this example, the parts by weight are in g. The Atractylodis macrocephalae rhizoma in this example is stir-fried Atractylodis macrocephalae rhizoma and roasted Atractylodis macrocephalae rhizoma, and the control example 5 is raw Atractylodis macrocephalae rhizoma.

[0071] This embodiment provides the traditional Chinese medicine hangover prescription for preventing and alleviating acute drunkenness. Among them, flos puerariae and rhizoma cimicifugae in this prescription are monarch drugs. Flos puerariae can relieve hangover and invigorate the spleen, effectively treat symptoms such as feverish thirst, headache, dizziness, vomiting and acid regurgitation, loss of appetite caused by alcohol poisoning, and it has the effects of clearing heat and detoxifying and diuresis, which helps to treat symptoms such as diarrhea and dysentery caused by intestinal inflammation induced by alcohol toxicity, and promotes the excretion of alcohol from urine. Rhizoma cimicifugae can clear heat and detoxify during the process of acute alcohol poisoning, effectively inhibit acute liver injury and gastrointestinal injury caused by alcohol, and has a certain analgesic effect on headache after drinking. The combination of flos puerariae and rhizoma cimicifugae is a hangover and detoxifying group. One mainly relieves alcohol toxicity, and the other mainly disperses alcohol toxicity, synergistically enhancing the hangover power and enabling the alcohol toxicity to be eliminated from both inside and outside.

[0072] Sedum sarmentosum Bunge, alisma orientale (Sam.) Juz., atractylodes macrocephala Koidz., poria cocos (Schw.) Wolf in this prescription are ministerial drugs in total. Sedum sarmentosum Bunge can relieve symptoms such as fever, thirst, sore throat, damp-heat in the liver and gallbladder, and jaundice caused by it; it has a certain relieving effect on symptoms such as headache and swelling caused by vasodilation. In addition, Sedum sarmentosum Bunge also has effects such as protecting the liver, reducing transaminase, improving pancreatitis, immune regulation, anti-fatigue, and antioxidant. Agrimonia pilosa Ledeb., galangal, rhizoma lmperatae are assistant and guiding drugs in this prescription. The combination of alisma orientale (Sam.) Juz., poria cocos (Schw.) Wolf, and rhizoma lmperatae is a group for strengthening the spleen and promoting diuresis, strengthening the effects of clearing heat and promoting diuresis, and diuretic detoxification, promoting the excretion of alcohol and its metabolites, and reducing the burden on the liver. The combination of stir-fried / processed atractylodes macrocephala Koidz. and galangal is a group for warming the middle-jiao and regulating the stomach, focusing on strengthening the spleen and regulating the stomach, warming the middle-jiao to dispel cold, restoring the transporting and transforming functions of the spleen and stomach, resisting the damage of alcohol toxicity to the spleen and stomach, relieving gastrointestinal discomfort symptoms after drunkenness, and balancing cold and heat. The combination of Sedum sarmentosum Bunge and Agrimonia pilosa Ledeb. is a group for protecting the liver and tonifying deficiency. While detoxifying, it plays a role in strengthening the healthy qi and tonifying deficiency, enabling the body to maintain a good state during the hangover process and preventing excessive consumption of the healthy qi.

[0073] Example 2

[0074] This embodiment provides the analysis process and results of the corresponding action targets of the composition provided in Example 1.

[0075] The analysis process of this embodiment is as follows:

[0076] 1. Use the TCMSP database to obtain the compounds contained in each drug in the hangover prescription provided in Example 1, and perform ADME screening on the obtained compounds. Among them, ADME refers to absorption, distribution, metabolism, and excretion. The conditions for ADME screening are: oral bioavailability (OB) ≥ 30%, drug likeness (DL) ≥ 0.18 as key indicators to screen out the active ingredients of the hangover prescription. Determination of the hangover prescription ingredients - targets: Use the STITCH database to screen out 42 protein targets corresponding to the active ingredients (compounds).

[0077] 2. Determine the corresponding relationship between the components of the anti-alcoholism formula provided in Example 1 and the targets. Use the GeneCards database to collect the action targets related to alcoholism, merge and screen for duplicates, and cross the predicted targets of the anti-alcoholism formula components with the related targets of acute alcoholism to obtain 19 possible action targets for the traditional Chinese medicine anti-alcoholism formula to treat acute alcoholism. The obtained results are as follows Figure 1 shown. According to this example, when using the GeneCards database for retrieval, it is preferred to use "Acute alcohol Intoxication" and "Acute Ethanol Intoxication" as keywords.

[0078] Furthermore, use the metascape website and the String website to analyze the 19 overlapping targets. The results are as follows Figure 2 shown. Figure A is the enrichment result of biological processes (Gene Ontology); Figure B is the functional enrichment result. Figures A and B show that the 19 targets are respectively clustered in steroid metabolism, metabolism of organic hydroxy compounds, cholesterol metabolism, and alcohol metabolism pathways. Figure 2 The results show that the main action pathways of the anti-alcoholism formula provided in Example 1 are the steroid metabolism pathway with anti-inflammatory, anti-allergic, promoting protein synthesis, immunosuppressive, and anti-shock effects, the cholesterol metabolism pathway involving stabilizing cell membranes and regulating blood lipids, and the alcohol metabolism pathway that increases aldehyde dehydrogenase.

[0079] The analysis results using the Metascape website are as follows Figure 3 shown. Figure A is the protein-protein interaction network; Figure B is the MCODE component extracted from the protein-protein interaction network. Figure 3 The results show that the core genes are clustered in the steroid pathway, cholesterol, and alcohol metabolism pathways involving regulating blood lipids.

[0080] In DisGeNET, perform relevant disease analysis and prediction on the 19 targets of the anti-alcoholism formula for treating acute alcoholism. The results are as follows Figure 4 shown. Figure 4 It shows that the 19 targets of the anti-alcoholism formula provided in Example 1 for treating acute alcoholism are mainly concentrated in the treatment of hyperlipidemia, dementia, hypercholesterolemia, fatty liver, cognitive impairment, cardiovascular and cerebrovascular diseases, etc.

[0081] The analysis results of the target organs in PaGeneBase in this example are as follows Figure 5 shown. The results show that the main target organ of the anti-alcoholism formula for treating acute alcoholism is the liver.

[0082] The enrichment analysis results of TRRUST are as follows Figure 6As shown in the figure, the transcription factors affected by the anti-hangover formula provided in Example 1 mainly include SP1, ESR1, RELA (P65), NF-κb1 (P50), and STAT3. Specifically, in the cardiovascular system, ESR1 can reduce total cholesterol, increase high-density lipoprotein to regulate blood lipid disorders, reduce the formation of oxygen free radicals for antioxidant purposes, and dilate blood vessels to promote carbon monoxide excretion. On the other hand, ESR1 plays a protective role in the central nervous system: it has a protective effect on various nerve injuries including brain injury, oxygen-glucose deprivation, mitochondrial toxins, and glutamate receptor activation, and regulates synaptic plasticity. In addition, ESR1 has a regulatory effect on the function of the pancreas, for example, it can regulate the secretion of insulin by pancreatic β cells through direct and indirect actions; ESR1 can also regulate the metabolic processes of bone resorption and bone formation. In a mouse model of Parkinson's disease, inhibiting the expression of SP1 can significantly inhibit the production of inflammatory cytokines, reduce the apoptosis rate, and inhibit the activity of pro-apoptotic regulatory factors (P<0.05); the contents of inflammatory factors TNF-α, IL-1β, and IL-6 are significantly reduced; NF-κB and STAT3 are classical inflammation-related pathways, and inhibiting their activation helps to reduce extensive inflammatory factor damage and the body's stress response.

[0083] Example 3

[0084] This example provides an animal experiment to verify the efficacy of the anti-hangover formula provided in Example 1.

[0085] The preparation steps of the animal model are as follows:

[0086] Select 36 SPF-grade C57 / 6J mice, male, with an average body weight of (23±2) g. After the mice are adaptively raised, they are randomly divided into 6 groups, with 5-6 mice in each group.

[0087] Gastric perfusion with alcohol at 3 g / kg for 2 times to establish an acute liver injury model. Among them, the pure alcohol amount (calculated according to 25 g / only) is 25 g×3 g / 1000 g = 0.075 g of alcohol, and the converted amount of 56° Erguotou (g / ml) is: 0.075 / 0.56 / 0.8 = 0.1674 ml.

[0088] Anti-hangover formula: An adult with a body weight of 60 kg drinks 150 ml at a time, that is, 2.5 ml / kg. The mouse is 9 times that of a human, that is, 22.5 ml / kg. 22.5 ml×25 g / 1000 g = 0.5625 ml. The original formula of 150 ml is concentrated by decoction to 50 ml, and 0.187 ml is used for gastric perfusion. After removing the loss, the gastric perfusion dose each time is 0.2 ml.

[0089] The grouping treatment is as follows:

[0090] A. Acute impact group: Each mouse was intragastrically administered 0.2 ml of normal saline. After 30 min, each mouse was intragastrically administered 0.34 ml of 56° Erguotou (0.1674 ml for two times).

[0091] B. Acute impact + traditional Chinese medicine group: Each mouse was intragastrically administered 0.2 ml of traditional Chinese medicine hangover formula. After 30 min, each mouse was intragastrically administered 0.34 ml of 56° Erguotou (0.1674 ml for two times).

[0092] C. Acute impact + metadoxine group: Each mouse was intragastrically administered 0.2 ml of metadoxine solution. After 30 min, each mouse was intragastrically administered 0.34 ml of 56° Erguotou (0.1674 ml for two times).

[0093] D. Control group: Each mouse was intragastrically administered normal saline twice.

[0094] E. Acute toxicity group: Each mouse was intragastrically administered traditional Chinese medicine once a day, 0.2 ml, for 15 consecutive days.

[0095] F. Acute toxicity control group: Each mouse was intragastrically administered normal saline once a day, 0.2 ml, for 15 consecutive days.

[0096] After the above treatments on the mice, in this embodiment, the "righting reflex" test was used to judge the drunken and sober states, and the behavioral states of the mice were observed. By recording the drunken and sober states of the mice in each group at different time points (0.5 h, 1 h, 2 h, 3 h, 4 h, 5 h, 6 h), the intervention effect of the hangover formula on the drunkenness of the mice was judged. The drunken and sober states recorded for each group were converted into scores through the corresponding relationship shown in Figure 7 and analyzed accordingly. Among them, Figure A is the score relationship table corresponding to the drunken and sober states; Figure B is the score situation of the mice at different time points. The drunkenness of the mice was judged by the disappearance of the righting reflex: If the mouse maintained the back-down posture for more than 30 s, it was considered that the righting reflex disappeared, that is, the mouse was drunk.

[0097] The statistical results and activity states of the activity abilities of the mice in different treatment groups over time are as shown in Figure 8 shown. Figure 8For the comparison results of the mouse states in the selected acute impact group, acute impact + traditional Chinese medicine group, acute impact + metadoxine group, and control group, in Figure A, the abscissa represents different time points, and the ordinate represents the activity ability (i.e., the scores corresponding to the drunk and sober states). The activity ability of the mice in the acute impact group was relatively low initially (the score was about 1). As time went by, the activity ability of the mice gradually increased. However, compared with the control group mice, the gap between the acute impact group mice and returning to normal was relatively large. The activity ability of the mice in the acute impact + traditional Chinese medicine group was relatively high initially (the score was about 2). As time passed, the activity ability of the mice gradually increased. By the 6th h, the activity ability of the mice had basically returned to normal. Compared with the mice in the acute impact + metadoxine group, the activity ability of the acute impact + traditional Chinese medicine group was higher, and as time went by, the activity ability of the mice recovered faster, indicating that the anti-alcoholism formula significantly prolonged the drunken latency period and shortened the sobering-up time. Figures B to D respectively show the activity states of the mice in the acute impact group, acute impact + traditional Chinese medicine group, acute impact + metadoxine group, and control group at 2 h, 4 h, and 6 h (the control group is not shown).

[0098] After the observation of the mouse behavioral states ended, the blood biochemical indexes of the mice were detected. Specifically, after the experiment ended, blood was taken, serum was separated, and the expression levels of liver function and blood lipids in the serum were detected. The results are as Figure 9 shown. In the acute impact group, alanine aminotransferase (ALT, Figure A) and aspartate aminotransferase (AST, Figure B) increased significantly; compared with the acute impact group, ALT and AST in the acute impact + traditional Chinese medicine group and acute impact + metadoxine group decreased significantly. At the same time, the degree of decrease in ALT and AST in the acute impact + traditional Chinese medicine group was greater than that in the acute impact + metadoxine group. In the acute impact group, triglyceride (TG, Figure C) and total cholesterol (TC, Figure D) increased; compared with the acute impact group, TG and TC in the acute impact + traditional Chinese medicine group and acute impact + metadoxine group decreased significantly.

[0099] This example also detected the blood biochemical indexes of the mice in the 15-day acute toxicity experiment. The results are as Figure 10 shown. Figure 10 It shows that there was no significant difference in AST (Figure B) between the control group and the acute toxicity group. However, compared with the control group, the acute toxicity group could reduce the ALT of the mice (p < 0.05) (Figure A). There was no significant difference in TG (Figure D) and TC (Figure C) between the control group and the acute toxicity group. Therefore, the traditional Chinese medicine anti-alcoholism formula provided in Example 1 has no significant toxicity evidence to the body and is a safe anti-alcoholism drug.

[0100] This example also collected the serum of each group of mice and used luminex liquid phase chip to detect the release levels of inflammatory factors interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and interleukin-10 (IL-10) in the serum. The results are asFigures 11 - 13 as shown Figure 11 The results showed that compared with the control group, the release level of IL-6 in the serum of mice in the acute shock group was significantly increased. Compared with the acute shock group, the release level of IL-6 in the serum of mice in the acute shock + traditional Chinese medicine group was significantly decreased. At the same time, compared with the acute shock + traditional Chinese medicine group, the release level of IL-6 in the serum of mice in the acute shock + metadoxine group was significantly increased. There were significant differences in the release levels of IL-6 between the acute shock + traditional Chinese medicine group and the acute shock group and the acute shock + metadoxine group (p < 0.05). Figure 12 The results showed that compared with the control group, the release level of TNF-α in the serum of mice in the acute shock group was significantly increased. Compared with the acute shock group, the release level of TNF-α in the serum of mice in the acute shock + traditional Chinese medicine group was significantly decreased. There was a significant difference in the release level of TNF-α between the acute shock + traditional Chinese medicine group and the acute shock group (p < 0.05). Figure 13 The results showed that compared with the control group, the release level of IL-10 in the serum of mice in the acute shock group was significantly increased. Compared with the acute shock group, the release level of IL-10 in the serum of mice in the acute shock + traditional Chinese medicine group was significantly decreased. There was a significant difference in the release level of IL-10 between the acute shock + traditional Chinese medicine group and the acute shock group (p < 0.05).

[0101] IL-6 and TNF-α are pro-inflammatory factors, and IL-10 is an anti-inflammatory factor. The secretion of IL-10 is usually driven by the NF-κB or STAT3 signaling pathways activated by pro-inflammatory factors (such as TNF-α, IL-6, etc.). Early anti-inflammatory treatment can rapidly inhibit the release of pro-inflammatory factors, resulting in the interruption of the induction signal of IL-10. The function of IL-10 is to inhibit excessive inflammation. When anti-inflammatory treatment effectively controls inflammation, the body reduces the secretion of IL-10 through negative feedback to avoid excessive immunosuppression. If anti-inflammatory treatment blocks the inflammatory cascade reaction in the early stage, it may directly inhibit the subsequent expression of IL-10. Within 24 hours of the acute inflammatory response induced by alcohol, after anti-inflammatory treatment with the anti-alcoholism formula provided in Example 1, the level of IL-10 decreased instead. The decrease in the level of IL-10 reflects the effectiveness of the anti-alcoholism formula in treating the inflammatory response induced by alcohol after pre-gastric administration, which is expressed as: the weakening of the pro-inflammatory signal leads to a decrease in IL-10 induction, and the decrease in IL-10 induction enables the rapid control of the inflammatory response without the need for a continuously high level of IL-10 to balance the immune response. Therefore, Figures 11 - 13 the results showed that the traditional Chinese medicine anti-alcoholism formula in Example 1 successfully blocked the positive feedback loop of inflammation.

[0102] Signal transducer and activator of transcription 3 (STAT3) is an important component of the JAK-STAT signaling pathway and plays a key role in the inflammatory response. STAT3 participates in the initiation, amplification, and resolution of the inflammatory response by regulating the expression of inflammatory factors, cell proliferation, differentiation, and apoptosis. IL-6 is one of the main activators of STAT3. After binding to the receptor, IL-6 phosphorylates STAT3 through JAK kinases, promoting its nuclear translocation and downstream gene transcription. After activation, STAT3 further upregulates the expression of IL-6, forming a positive feedback loop to amplify the inflammatory response. TNF-α can indirectly activate STAT3 through the NF-κB pathway, enhancing the inflammatory response. STAT3 can also affect the intensity and duration of the inflammatory response by regulating the expression of TNF-α. IL-10 is an important downstream target gene of STAT3. After activation, STAT3 promotes the expression of IL-10, exerting an anti-inflammatory effect. IL-10 inhibits the expression of pro-inflammatory factors (such as IL-6, TNF-α) by activating STAT3, promoting the resolution of the inflammatory response.

[0103] In this example, liver tissues of mice in each group were taken, proteins were extracted, and the expression of STAT3 protein was detected by immunoblotting, as Figure 14 shown. Figure 14 It was shown that the expression level of phosphorylated-STAT3 (p-STAT3) was significantly increased in the liver tissues of the acute shock group; compared with the acute shock group, the expression level of phosphorylated-STAT3 (p-STAT3) was significantly decreased in the acute shock + traditional Chinese medicine group and the acute shock + metadoxine group. The STAT3 signaling pathway has a dual role in the inflammatory response: on the one hand, it amplifies the inflammatory signal by promoting the expression of pro-inflammatory factors (such as IL-6, TNF-α, etc.); on the other hand, it inhibits the excessive inflammatory response by upregulating the expression of anti-inflammatory factors (such as IL-10). p-STAT3 maintains the balance of the inflammatory response and prevents excessive inflammatory damage by regulating the expression of pro-inflammatory factors (such as IL-6, TNF-α) and anti-inflammatory factors (such as IL-10). Combining the luminex inflammatory factor detection ( Figures 11 - 13 ) and WB results showed that the traditional Chinese medicine formula for relieving alcoholism inhibited the activation of the STAT3 signaling pathway and downregulated IL-6 and TNF-α; the weakened pro-inflammatory signal led to a decrease in IL-10 induction. Figure 14 The results showed that the inflammatory response caused by acute alcohol injury was effectively controlled at an early stage in the acute shock + traditional Chinese medicine group and the acute shock + metadoxine group. Targeting the STAT3 signaling pathway may provide a new strategy for the treatment of inflammatory diseases.

[0104] Furthermore, this embodiment also provides an observation study on the liver pathological tissues of each group of mice. Specifically, liver tissue samples of each group of mice are taken. After the samples are fixed with 4% paraformaldehyde and in good fixed state, they are trimmed, dehydrated, embedded, sectioned, stained, mounted, and finally examined under a microscope for qualified slides in strict accordance with the SOP procedure of the pathological experiment in this unit. The sections are viewed under a microscope (upright white light photography microscope, Nikon (Japan), Eclipse Ci-L), and the tissue sections are observed in detail at different magnifications. The pathological scoring criteria for the sections in this embodiment ([US] Peter. Mann, etc. International Nomenclature of Pathology Changes in Rats and Mice and Diagnostic Criteria (INHAND) [M]. Translated by Yang Lifeng, Zhou Xiangmei, Zhao Deming. Beijing: China Agricultural Press, 2019) are as follows: The level within the normal range (under the research conditions, considering factors such as the age, gender, and strain of the animals, the tissue is considered normal. Changes occurring under other conditions can be considered abnormal) is 0; the level of very slight (the changes just exceed the normal range) is 1; the level of slight (lesions can be observed, but not yet severe) is 2; the level of moderate (the lesions are obvious and are likely to be more severe) is 3; the level of severe (the lesions are very severe / the lesions have occupied the entire tissue or organ) is 4. On the other hand, in this embodiment, the pathological tissues are judged from the aspects of congestion, stasis, hemorrhage, edema, degeneration, necrosis, hyperplasia, fibrosis, organization, granulation tissue, inflammatory changes, etc., and the typical lesion characteristics are marked with arrows of different colors in the photographed section pictures. The scores of the pathological tissues of each group of mice and the results of the pathological sections are as Figure 15 and Figure 16 shown.

[0105] The pathological scores of the mouse liver tissues are carried out from three dimensions of degeneration, necrosis, and inflammatory cell infiltration. Figure 15 The results show that the degree of injury in the acute shock group is the most severe, with an average score of 2.667; the degree of injury in the acute shock + metadoxine group is the second, with an average score of 2.0; the average score of the injury degree in the acute shock + traditional Chinese medicine group is 1.200, which is significantly lower than the injury degree score of the acute shock group (p < 0.05). Figure 15 The results indicate that the traditional Chinese medicine formula for relieving alcohol has an obvious protective ability on the liver.

[0106] Representative pathological sections of each group are selected as Figure 16As shown, the scale bar = 100 μm. In the liver tissue of mice in the acute impact + traditional Chinese medicine group, in the visual field, the central vein (black arrow) is at the center of the liver lobule of the liver tissue, and around it are hepatocytes (blue arrow) and hepatic sinusoids (yellow arrow) arranged roughly radially. The hepatocytes are round and plump; the hepatic plates are arranged regularly and neatly, and there is no obvious dilation or compression of the hepatic sinusoids; there is no obvious abnormality in the portal area between adjacent liver lobules; no obvious inflammatory cell infiltration is seen. In the liver tissue of mice in the acute impact + metadoxine group, in the visual field, a large number of hepatocytes show slight vacuolar degeneration (yellow arrow) can be seen around the central vein, portal area and in the hepatic parenchyma, and tiny round vacuoles can be seen in the cytoplasm; quite a number of hepatocytes show slight hydropic degeneration, and the cytoplasm is loose and lightly stained; no obvious necrosis is seen, and no obvious inflammatory cell infiltration is seen. In the liver tissue of mice in the acute impact group, in the visual field, a relatively large number of hepatocytes show slight vacuolar degeneration (yellow arrow) can be seen around the central vein, portal area and in the hepatic parenchyma, and tiny round vacuoles can be seen in the cytoplasm; occasional hepatocyte necrosis (red arrow) can be seen around blood vessels, the cell nucleus is fragmented, and occasional granulocyte infiltration (blue arrow) can be seen around it. Figure 16 The results also show that the traditional Chinese medicine anti-alcoholism formula provided in Example 1 has obvious protective ability for the liver.

[0107] In addition, in this example, pathological scoring of gastric mucosal damage and observation and research of pathological tissues were also carried out on each group of mice, and the results are as Figure 17 and Figure 18 shown. Pathological scoring of the gastric tissues of each group of mice was carried out from four dimensions of necrosis, inflammatory cell infiltration, bleeding, and dilation of gastric glands, and the results are as Figure 17 shown. The results show that the degree of injury in the acute impact + metadoxine group is the most serious, with an average score of 3.0; the degree of injury in the acute impact group is the second, with an average score of 2.0; the degree of injury in the acute impact + traditional Chinese medicine group is the lowest, with an average score of 1.200. Therefore, the traditional Chinese medicine anti-alcoholism formula provided in Example 1 has a certain protective ability for the gastric mucosa. Representative pathological sections of each group are as Figure 18 shown.

[0108] Figure 18Pathological sections of the gastric mucosa of mice in each group, scale bar = 100 μm. In the gastric mucosa tissue of mice in the acute impact + traditional Chinese medicine group, there was rare necrosis and exfoliation of mucosal epithelial cells (red arrow) in the mucosal layer of the gastric tissue within the visual field, pyknosis and fragmentation of cell nuclei; the number of gastric glands in the lamina propria was abundant and arranged closely. The gastric glands were tubular glands composed of chief cells and parietal cells; the submucosa was connective tissue; a small amount of lymphocyte infiltration was visible (blue arrow), and no obvious inflammatory cell infiltration was seen. In the gastric mucosa tissue of mice in the acute impact + metadoxine group, there was small-area necrosis (red arrow) in the superficial layer of the mucosal layer of the gastric tissue within the visual field, disappearance of epithelial cells and gastric gland structure, slight bleeding (yellow arrow), a small amount of red blood cells were visible, and a small amount of granulocyte infiltration was accompanied around (blue arrow); the number of gastric glands in the lamina propria was abundant, and slight dilation of gastric glands was rarely seen (green arrow), and the submucosa was connective tissue. In the gastric mucosa tissue of mice in the acute impact group, there was rare necrosis and exfoliation of mucosal epithelial cells in the mucosal layer of the gastric tissue within the visual field, pyknosis and fragmentation of cell nuclei; the number of gastric glands in the lamina propria was abundant and arranged closely. The gastric glands were tubular glands composed of chief cells and parietal cells; the submucosa was connective tissue, and a small amount of lymphocyte infiltration was visible (blue arrow); the number of gastric glands in the lamina propria was abundant, and slight dilation of gastric glands was rarely seen (green arrow).

[0109] Example 4

[0110] This example provides the preparation method of Atractylodes macrocephala, process optimization steps such as reflux purification, etc.

[0111] Fried Atractylodes macrocephala and roasted Atractylodes macrocephala are Chinese medicinal materials processed from Atractylodes macrocephala through different processing methods. There are certain differences in their preparation methods and functions, which are as follows:

[0112] (1) Preparation of fried Atractylodes macrocephala

[0113] Select Atractylodes macrocephala medicinal materials that are dry, free of impurities, and of uniform size. Remove impurities such as fibrous roots, wash them, slice them after being moistened thoroughly. Generally, cut them into thin slices with a thickness of 2 - 4 mm.

[0114] Heat the pot, pour in an appropriate amount of yellow soil or wheat bran, heat with medium heat, and when it starts to smoke, put in the Atractylodes macrocephala slices. Continuously turn the Atractylodes macrocephala slices to make them fully contact with the yellow soil or wheat bran and be evenly heated. The frying time is generally 5 - 10 minutes. When the surface of the Atractylodes macrocephala slices turns yellowish-brown, has scorched spots, and emits an aroma, take them out.

[0115] Sift the fried Atractylodes macrocephala slices with a sieve to remove the wheat bran or yellow soil, and then obtain fried Atractylodes macrocephala after cooling.

[0116] (2) Preparation of roasted Atractylodes macrocephala

[0117] Select high-quality Atractylodes macrocephala, wash it, moisten it thoroughly and then slice it. Prepare honey, generally select high-quality, impurity-free natural honey, and if necessary, refine the honey appropriately to make its viscosity and medicinal properties more suitable for the requirements of processing.

[0118] Wash and heat the pot, pour in an appropriate amount of refined honey, heat it over low heat. When the honey melts and uniform small bubbles appear, add the Atractylodes macrocephala slices. Gently turn the Atractylodes macrocephala slices to evenly coat the surface of the slices with honey, and continue to heat and roast over low heat. The roasting time is usually 10 - 15 minutes, and during this period, keep turning to prevent the Atractylodes macrocephala slices from getting burnt.

[0119] When the color of the Atractylodes macrocephala slices deepens, the surface is moist and shiny, and it is not sticky to the touch, take them out, place them in a ventilated place to dry or dry them at a low temperature to obtain roasted Atractylodes macrocephala.

[0120] (3)Preparation and purification of the first Atractylodes macrocephala enzymatic hydrolysate and the second Atractylodes macrocephala enzymatic hydrolysate

[0121] Take 12 g of raw Atractylodes macrocephala, divide it into 2 equal parts, stir-fry or honey-roast them respectively, and the preparation methods are the same as in (1) and (2); after stir-frying or honey-roasting, add them to the soaking water respectively and decoct at 70 °C for 15 minutes to obtain the first Atractylodes macrocephala liquid and the second Atractylodes macrocephala liquid; cool the first Atractylodes macrocephala liquid and the second Atractylodes macrocephala liquid after decoction to 40 - 50 °C respectively, and then add cellulase, protease, and amylase with a mass ratio of 1.5:1:2 for enzymatic hydrolysis, and then carry out reflux extraction and purification to obtain the first Atractylodes macrocephala enzymatic hydrolysate and the second Atractylodes macrocephala enzymatic hydrolysate.

[0122] After enzymatic hydrolysis of Atractylodes macrocephala by cellulase, protease, and amylase, it has many benefits in terms of improving the extraction rate of active ingredients, enhancing pharmacological activity, improving absorption and utilization rate, and reducing adverse reactions.

[0123] Example 5

[0124] This example provides a preparation method of the traditional Chinese medicine hangover prescription in Example 1, which is as follows:

[0125] 30 g of Pueraria flower, 10 g of Rhizoma Cimicifugae, 10 g of Sedum sarmentosum Bunge, 15 g of Alisma orientale (Sam.) Juzep., 12 g of Atractylodes macrocephala, 30 g of Poria cocos (Schw.) Wolf, 15 g of Agrimonia pilosa Ledeb., 15 g of Alpinia officinarum Hance, 30 g of Imperata cylindrica Beauv. Except for Atractylodes macrocephala, soak the rest of the raw medicinal materials in 1.5 times the amount of water for 2 hours respectively and then decoct. After decocting for 30 minutes, collect the medicinal liquid, cool it to 40 - 50 °C, and then add the first Atractylodes macrocephala enzymatic hydrolysate and the second Atractylodes macrocephala enzymatic hydrolysate to obtain the traditional Chinese medicine hangover prescription.

[0126] Comparative Example 1

[0127] 10 g of Pueraria flower, 5 g of Rhizoma Cimicifugae, 10 g of Sedum sarmentosum Bunge, 15 g of Alisma orientale (Sam.) Juzep., 12 g of Atractylodes macrocephala, 30 g of Poria cocos (Schw.) Wolf, 15 g of Agrimonia pilosa Ledeb., 15 g of Alpinia officinarum Hance, 30 g of Imperata cylindrica Beauv.

[0128] In this comparative example, the dosage of the group for relieving hangover and detoxifying was reduced, that is, the amounts of Puerariae Flos and Cimicifugae Rhizoma were reduced.

[0129] Comparative Example 2

[0130] Puerariae Flos 30 g, Cimicifugae Rhizoma 10 g, Sedum sarmentosum Bunge 10 g, Alismatis Rhizoma 5 g, Atractylodis Macrocephalae Rhizoma 12 g, Poria 5 g, Agrimoniae Herba 15 g, Alpiniae Officinarum Rhizoma 15 g, Imperatae Rhizoma 5 g.

[0131] In this comparative example, the dosage of the group for strengthening the spleen and promoting diuresis was reduced, that is, the amounts of Alismatis Rhizoma, Poria and Imperatae Rhizoma were reduced.

[0132] Comparative Example 3

[0133] Puerariae Flos 30 g, Cimicifugae Rhizoma 10 g, Sedum sarmentosum Bunge 10 g, Alismatis Rhizoma 15 g, Atractylodis Macrocephalae Rhizoma 6 g, Poria 30 g, Agrimoniae Herba 15 g, Alpiniae Officinarum Rhizoma 5 g, Imperatae Rhizoma 30 g.

[0134] In this comparative example, the dosage of the group for warming the middle-jiao and regulating the stomach was reduced, that is, the amounts of Atractylodis Macrocephalae Rhizoma and Alpiniae Officinarum Rhizoma were reduced.

[0135] Comparative Example 4

[0136] Puerariae Flos 30 g, Cimicifugae Rhizoma 10 g, Sedum sarmentosum Bunge 5 g, Alismatis Rhizoma 15 g, Atractylodis Macrocephalae Rhizoma 12 g, Poria 30 g, Agrimoniae Herba 5 g, Alpiniae Officinarum Rhizoma 15 g, Imperatae Rhizoma 30 g.

[0137] In this comparative example, the dosage of the group for protecting the liver and tonifying deficiency was reduced, that is, the amounts of Sedum sarmentosum Bunge and Agrimoniae Herba were reduced.

[0138] Comparative Example 5

[0139] Puerariae Flos 30 g, Cimicifugae Rhizoma 10 g, Sedum sarmentosum Bunge 10 g, Alismatis Rhizoma 15 g, Atractylodis Macrocephalae Rhizoma recens 12 g, Poria 30 g, Agrimoniae Herba 15 g, Alpiniae Officinarum Rhizoma 15 g, Imperatae Rhizoma 30 g. The raw medicinal materials were respectively soaked in 1.5 times of clear water for 2 hours and then decocted, and the medicinal juice was taken after 30 minutes, which was the hangover-relieving prescription of this comparative example.

[0140] Eighteen male drinkers were selected and given one dose orally before, during or after drinking, and their drunkenness time and improvement of TCM syndromes were observed. The specific results are shown in Table 1.

[0141] Table 1

[0142]

[0143]

[0144] It should be noted that the above specific embodiments are exemplary. Those skilled in the art can come up with various solutions inspired by the disclosure of the present invention, and these solutions also fall within the scope of the disclosure of the present invention and within the protection scope of the present invention. Those skilled in the art should understand that the specification and drawings of the present invention are illustrative and do not constitute a limitation to the claims. The protection scope of the present invention is defined by the claims and their equivalents. The specification of the present invention contains multiple inventive concepts. For example, "preferably" and "according to a preferred embodiment" indicate that the corresponding paragraphs disclose an independent concept. The applicant reserves the right to file divisional applications based on each inventive concept. Throughout the text, the features guided by "preferably" are only optional and should not be construed as must be provided. Therefore, the applicant reserves the right to waive or delete the relevant preferred features at any time.

Claims

1. A composition for preventing and relieving acute drunkenness, characterized in that: The composition is prepared from the following raw materials in parts by weight: 30 parts of kudzu flower, 10 parts of cimicifuga heracleifolia, 10 parts of scutellaria baicalensis, 15 parts of oriental alisma, 15 parts of atractylodes macrocephala, 30 parts of tuckahoe, 15 parts of agrimony, 15 parts of galangal and 30 parts of imperata rhizome.

2. The composition according to claim 1, characterized in that The Atractylodes macrocephala is stir-fried and / or roasted.

3. A method for preparing a composition for preventing and relieving acute drunkenness, comprising the following steps: The raw medicinal materials except Atractylodes macrocephala are soaked in water and then decocted, the medicinal liquid is collected and cooled, and then the Atractylodes macrocephala enzymatic hydrolysate is added to obtain the composition.

4. Use of the composition according to claim 1 or 2 and the preparation method of the composition according to claim 3 in preparing a substance for prolonging the latent period of drunkenness and / or shortening the sobering time.

5. Use of the composition according to claim 1 or 2 and the preparation method of the composition according to claim 3 in preparing substances for treating inflammatory diseases.

6. Use of the composition according to claim 1 or 2 and the preparation method of the composition according to claim 3 in reducing the release level of inflammatory factors.

7. The use according to claim 6, characterized in that: Inflammatory factors include one or more of interleukin-6, tumor necrosis factor-α and interleukin-10.

8. Use of the composition according to claim 1 or 2 and the preparation method of the composition according to claim 3 in inhibiting the activation of transcription activator 3 signaling pathway.

9. Use of the composition according to claim 1 or 2 and the preparation method of the composition according to claim 3 in preparing a substance for treating liver damage.

10. Use of the composition according to claim 1 or 2 and the preparation method of the composition according to claim 3 in preparing a substance for treating gastric mucosal damage.

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