Pesticide composition and application thereof

Abstract

The invention belongs to the field of agricultural bactericides, and particularly relates to a pesticide composition and application thereof. The composition comprises a component A and a component B, the component A is fenaminstrobin, and the component B is validamycin; the weight ratio of the component A to the component B is (1: 80)-(80: 1). The pesticide composition disclosed by the invention has a very good prevention and treatment effect on damping-off, epidemic diseases, root rot, fusarium wilt, verticillium wilt, sclerotiniose, sheath blight, damping-off and gray mold, and has a very good synergistic effect. According to the invention, pesticides with different action mechanisms are combined for use, the pesticide composition has a very good prevention and treatment effect on the diseases, the generation of drug resistance of pathogenic bacteria can be delayed, the service life of the pesticide is prolonged, and meanwhile, chemical pesticides and biological pesticides are mixed for use, so that the use amount of the chemical pesticides can be reduced. In agricultural production, the two pesticides are used together according to the proper proportion provided by the invention, and the effects of saving labor and time can also be achieved.

Description

Technical Field

[0001] The present invention belongs to the field of agricultural fungicides, and particularly relates to a pesticide composition and its application. Background Art

[0002] Fenaminstrobin was discovered by Shenyang Research Institute of Chemical Industry in 1999 and belongs to the methoxyacrylate fungicides. Fenaminstrobin has a broad fungicidal spectrum, high activity, and has preventive and therapeutic effects. It can be used to control various plant diseases such as wheat rust, wheat powdery mildew, rice sheath blight, false smut of rice, cucumber powdery mildew, cucumber downy mildew, grape downy mildew, apple blotch leaf spot, apple powdery mildew, banana leaf spot, tomato early blight, pear scab, strawberry powdery mildew, sunflower rust, etc. And it has a certain improvement effect on the growth traits and quality of crops.

[0003] Validamycin is an antibiotic produced by actinomycetes, with strong systemic absorption, easily absorbed by fungal cells and rapidly conducted within them, interfering with and inhibiting the growth and development of fungal cells. This fungicide is mainly used for rice sheath blight, and can also be used for the control of rice false smut, large and small leaf spots of corn, and crop diseases of vegetables, cotton, beans, etc. In addition, studies have shown that validamycin has a significant inhibitory effect on the biosynthesis of DON toxin of Fusarium graminearum, and in addition to directly acting on pathogenic bacteria, validamycin can also be used as a plant immune activator to induce plants to produce resistance to pathogenic bacteria.

[0004] Studies have shown that the long-term, large-scale and frequent use of the same type of pesticides will cause the generation of pathogen resistance, and the environmental problems caused by the current use of chemical pesticides are also receiving increasing attention. Therefore, the mixed use of chemical pesticides and biological pesticides can not only delay the generation of resistance, but also reduce the use of chemical pesticides to achieve the purpose of environmental friendliness.

[0005] At present, there is no relevant report on the compounding of fenaminstrobin and validamycin. Summary of the Invention

[0006] The purpose of the present invention is to provide a fungicidal composition, a disease-preventing pesticide composition with a synergistic effect, low use cost and good control effect. At the same time, the mixed use of chemical pesticides and biological pesticides can reduce the usage amount of chemical pesticides.

[0007] To achieve the above purpose, the technical solution adopted by the present invention is:

[0008] A pesticide composition, the composition is active ingredient A and active ingredient B; active ingredient A is fenaminstrobin, and active ingredient B is validamycin; the weight ratio between the two components of active ingredient A and active ingredient B is 80:1 - 1:80.

[0009] The weight ratio between the active component A and the active component B is 10:1-1:10.

[0010] The application of the pesticide composition, and the application of the composition as a fungicide in agriculture, forestry and planting.

[0011] The composition is used as a fungicide in agriculture, forestry and planting for preventing and controlling damping-off disease, blight, root rot, wilt, verticillium wilt, sclerotinia rot, sheath blight, damping-off disease or gray mold.

[0012] The composition is used as a fungicide in agriculture, forestry and planting industries for preventing and controlling sheath blight or damping-off caused by Rhizoctonia fungi and gray mold caused by Botrytis fungi.

[0013] The composition is used as a fungicide in agriculture, forestry and planting for preventing and controlling rice sheath blight, rice damping-off, cotton damping-off, corn sheath blight, wheat sheath blight and vegetable gray mold.

[0014] The method for using the composition of the present invention is to prepare component A and component B in advance according to the above appropriate ratio or prepare them on the spot, or use them separately in sequence, all of which show significant disease prevention effects.

[0015] A pesticide fungicide, the fungicide contains the pesticide composition, takes the pesticide composition as an active ingredient, and the active ingredient accounts for 0.1-95% of the mass of the fungicide.

[0016] The advantages of the present invention are:

[0017] The composition of the present invention is compounded and mixed with fenthionil and jinggangmycin, so that it has a good synergistic effect and significantly improves the disease prevention effect. The present invention uses a combination of fungicides with different action mechanisms, which not only has excellent prevention and control effects, but also can delay the generation of drug resistance of pathogens and extend the service life of the agents. At the same time, the use of chemical pesticides and biological pesticides can reduce the use of chemical pesticides. In agricultural production, the two fungicides are used together according to the appropriate ratio proposed by the present invention, which can also achieve the effect of saving labor and time. DETAILED DESCRIPTION

[0018] The synergistic effect of the composition of the present invention can be further illustrated by the following examples, but the present invention is by no means limited thereto. The active components described therein are the pesticide compositions A and B of the present invention, component A is enoxamyl, and component B is validamycin; and all substances can be purchased on the market.

[0019] The test methods and evaluation methods are as follows:

[0020] Indoor in vitro test: In a sterile operating table, dissolve component A with acetone and component B with sterile water, and dilute them with sterile water to the test solution with the required concentration. Then, mix the test solutions of component A and B with the required concentration prepared respectively according to the set ratio to prepare the test solution of the composition. Cool the melted culture medium to 60°C - 70°C, add a quantitative medicament according to the set concentration to prepare a toxic culture medium containing different dosages of the medicament. After it cools sufficiently, inoculate a test pathogen disc with a diameter of 0.5 cm, and set a blank control. Place it in an incubator for cultivation (Rhizoctonia solani at 28°C ± 1°C, Botrytis cinerea at 25°C ± 1°C). During the investigation, use the cross method to measure the growth diameter of the test pathogen colony for each treatment respectively, and calculate the inhibition rate.

[0021] Use the DPS data processing system to calculate the virulence regression equation of each medicament, and obtain the EC 50 value of each test medicament and its ratio. Obtain the co-toxicity coefficient according to the method of SUN-Johnson to compare the virulence effects of each medicament, so as to screen out the best synergistic ratio.

[0022]

[0023]

[0024] Theoretical virulence index of the mixture = virulence index of medicament A × percentage of active ingredient of medicament A in the mixture + virulence index of medicament B × percentage of active ingredient of medicament B in the mixture

[0025] Co-toxicity coefficient CTC: greater than 120 is synergistic, less than 80 is antagonistic, and 80 to 120 is additive.

[0026] In vivo pot experiment: Dissolve component A with acetone and component B with sterile water, and dilute them with 0.1% Tween-80 water to the test solution with the required concentration. Then, mix the test solutions of component A and B with the required concentration prepared respectively according to the set ratio to prepare the test solution of the composition. Then, conduct experiments with single components with different effective concentrations (effective concentrations of 100, 50, 25, 12.5, 6.25 mg / L) and compositions with different ratios. Spray the medicament evenly on rice seedlings, air dry naturally for 24 h, then inoculate the pathogen evenly on the soil surface, place it in a greenhouse for cultivation (RH > 85%, temperature 26 ± 2°C), cultivate for 8 d, and investigate the number of diseased plants after the blank control shows stable disease development, and calculate the disease plant rate and control effect. The calculation methods are as follows:

[0027]

[0028]

[0029] Example 1 Joint Toxicity Test against Rhizoctonia solani

[0030] The test was carried out according to the above indoor in vitro test method. The results showed that the composition had a good control effect against Rhizoctonia solani. When the ratio of enestroburin to jinggangmycin was in the range of 80:1 - 1:80, the co-toxicity coefficient was greater than 120, indicating a synergistic effect. Among them, when the ratio was in the range of 10:1 - 1:10, the synergistic effect was the most obvious. The results are shown in Table 1 for details.

[0031] Table 1 Results of Joint Toxicity Test of Individual Active Components and Composition against Rhizoctonia solani

[0032]

[0033] Example 2 Joint Toxicity Test against Botrytis cinerea

[0034] The test was carried out according to the above indoor in vitro test method. The results showed that the composition had a good control effect against Botrytis cinerea. When the ratio of enestroburin to jinggangmycin was in the range of 80:1 - 1:80, the co-toxicity coefficient was greater than 120, indicating a synergistic effect. Among them, when the ratio was in the range of 10:1 - 1:10, the synergistic effect was the most obvious. The results are shown in Table 2 for details.

[0035] Table 2 Results of Joint Toxicity Test of Individual Active Components and Composition against Botrytis cinerea

[0036]

[0037] Example 3 In Vivo Pot Experiment against Rhizoctonia solani

[0038] The test was carried out according to the above in vivo pot test method. The results showed that the composition had a good control effect against Rhizoctonia solani. When the ratio of enestroburin to jinggangmycin was in the range of 10:1 - 1:10, the control effect was significantly better than that of the single agent. The results are shown in Table 3 for details.

[0039] Table 3 Results of In Vivo Pot Experiment of Individual Active Components and Composition against Rhizoctonia solani

[0040]

Claims

1. A pesticide composition, characterized in that: The composition comprises active ingredient A and active ingredient B; active ingredient A is enestroburin, and active ingredient B is jinggangmycin; the weight ratio between the two components of active ingredient A and active ingredient B is 80:1 - 1:

80.

2. The pesticide composition according to claim 1, characterized in that: The weight ratio between the two components of active ingredient A and active ingredient B is 10:1 - 1:

10.

3. The application of the pesticide composition according to claim 1, characterized in that: The application of the said composition in fungicides for agriculture, forestry and planting.

4. The application of the pesticide composition according to claim 1, characterized in that: The application of the said composition in fungicides for agriculture, forestry and planting for preventing and controlling damping-off, phytophthora blight, root rot, fusarium wilt, verticillium wilt, sclerotinia rot, sheath blight, damping-off or gray mold.

5. The application of the pesticide composition according to claim 4, characterized in that: The application of the said composition in fungicides for agriculture, forestry and planting for preventing and controlling sheath blight or damping-off caused by fungi of the genus Rhizoctonia, and gray mold caused by fungi of the genus Botrytis.

6. The application of the pesticide composition according to claim 5, characterized in that: The application of the said composition in fungicides for agriculture, forestry and planting for preventing and controlling sheath blight of rice, damping-off of rice, damping-off of cotton, sheath blight of corn, sheath blight of wheat, and gray mold of vegetables.

7. A pesticide bactericide, characterized in that: The fungicide contains the pesticide composition as claimed in claim 1, taking the pesticide composition as claimed in claim 1 as the active ingredient, and the active ingredient accounts for 0.1 - 95% of the mass of the fungicide.

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