Nutrient enrichment chicken feed and preparation method thereof

Abstract

The invention relates to a nutrient enrichment chicken feed and a preparation method thereof, and belongs to the technical field of feed preparation, and the preparation method specifically comprises the following steps: selecting a composite strain, co-activating in an activation culture medium to obtain an activated strain, adding the activated strain into a culture medium, fermenting, and collecting a product. And mixing the fermentation product, a nutrition enhancer and a basal feed, and performing crushing and sieving to obtain the nutrition-enhanced chicken feed. Through fermentation of composite strains, complex organic matters are decomposed to generate amino acids, vitamins and the like which are easily absorbed by chickens, so that the nutritional value of the feed is improved, protein absorption of the old laying hens is promoted, and amino acid supply required by egg laying is maintained. In the composite strain, saccharomycetes, acetic bacteria and the like have a synergistic effect, calcium conversion is promoted, and immunity is enhanced. The nutrient supplement is added, feed components are optimized, the growth requirements of the chickens are met, and the production performance is improved. The problems that in the prior art, feed is insufficient in nutritional value and unreasonable in nutritional ingredient proportion, and the digestive ability of the old laying hens is reduced are solved.

Description

Technical Field

[0001] The present application relates to the technical field of feed preparation, and more specifically, to a nutritionally fortified chicken feed and a preparation method thereof. Background Art

[0002] In the current poultry farming industry, the quality and nutritional value of feed have a crucial impact on the growth performance and egg production efficiency of chickens. However, there are still some significant deficiencies in the existing chicken feed preparation technologies, especially in meeting the nutritional needs of old laying hens and improving their production performance.

[0003] Traditional chicken feed often relies on simple raw material mixing and lacks in-depth processing and optimization for the special physiological needs of old laying hens. As chickens age, their digestive ability gradually declines, and the absorption and utilization efficiency of protein decreases, which directly leads to problems such as reduced egg production and decreased egg quality. The existing feed preparation technologies have not effectively solved the contradiction between the decline in digestive ability and the increasing nutritional needs of old laying hens, thus affecting the breeding efficiency.

[0004] In addition, there are also limitations in the proportion and types of nutritional components in the existing feed. Although many feeds are rich in protein and other basic nutrients, they lack fine regulation for the specific nutritional needs during the growth process of chickens. For example, the proportion of key nutritional components such as amino acids and vitamins is often not reasonable enough to meet the nutritional support required for old laying hens to maintain a high egg production rate and good egg quality.

[0005] Based on the above statements, the present application provides a nutritionally fortified chicken feed and a preparation method thereof. Summary of the Invention

[0006] To solve the problems raised in the background art, the present application provides a nutritionally fortified chicken feed and a preparation method thereof. By introducing a compound strain fermentation technology, the proportion of nutritional components in the feed is optimized, and the nutritional value and utilization rate of the feed are improved, so as to meet the special nutritional needs of old laying hens during the growth process and promote their healthy growth and maintenance of a high egg production rate.

[0007] A nutritionally fortified chicken feed and a preparation method thereof include the following preparation steps: S1. Select a compound strain, and co-activate it in an activation medium to obtain an activated strain; S2. Add the activated strain to a culture medium, ferment and culture, and collect the fermentation product after fermentation; S3. Mix the fermentation product obtained in step S2, a nutritional fortifier, and a basic feed, pulverize and pass through a 60 - 80 mesh sieve to obtain a nutritionally fortified chicken feed; Among them, the compound strain includes yeast, acetic acid bacteria, lactic acid bacteria, and Neurospora crassa.

[0008] Further, in step S1, the activated strain is specifically prepared by the following steps: S11. Using MRS broth medium as the basic medium, add 2-3% carbon source, 0.01-0.03% magnesium sulfate, and 0.002-0.004% manganese sulfate by mass percentage to the basic medium, and adjust the pH value of the system to 5.0-5.8 to obtain the activation medium; S12. Inoculate the composite strain into the activation medium obtained in step S1 at an inoculation amount of 5-7%, set the culture temperature to 25-30°C, shake and culture on a shaker, add an inorganic calcium source at the 4th hour and 6th hour of fermentation, the shaker speed is 100-200 rpm, and the culture time is 12-24 hours to obtain the activated strain.

[0009] Further, in step S12, the composite strain is obtained by mixing yeast, acetic acid bacteria, lactic acid bacteria, and Neurospora crassa in a viable cell number ratio of 1:(0.2-0.5):(0.1-0.3):(0.5-1) in a sterile environment.

[0010] Further, in step S2, the operation of adding the activated strain to the culture medium, fermenting and culturing, and collecting the fermentation product after fermentation is as follows: S21. Prepare the culture medium according to the following formula: 5-10 wt% astragalus powder, 3-5 wt% medical stone, 50-60 wt% mixture of corn and corn cob, 0.3-0.5 wt% magnesium sulfate, 6-10 wt% inorganic calcium source, 6-10 wt% sodium dihydrogen phosphate, and the rest is soybean powder; S22. Then mix the activated strain with the culture medium in a mass ratio of 1:(8-10), add 50% of the total weight of the two of water, and then adjust the pH value of the system to 5.0-5.8. Then shake and culture the system on a shaker, add an inorganic calcium source at the 4th hour and 6th hour of fermentation, set the culture temperature to 25-30°C, the shaker speed is 120-150 rpm, and after fermenting for 55-75 hours in a dark and ventilated place, collect the mixture including the culture medium to obtain the fermentation product.

[0011] Further, in step S22, the addition amount of the inorganic calcium source each time is 0.3-0.5 wt% of the culture medium.

[0012] Further, in step S3, the nutritional fortifier is obtained by mixing 1-3 parts of fish meal, 0.3-0.5 parts of edible salt, 0.1-0.2 parts of lysine, 0.1-0.2 parts of methionine, and 0.3-0.6 parts of threonine.

[0013] Further, in step S3, the basic feed is at least one of millet, distillers' grains, soybean meal, soybean flour, corn, and wheat.

[0014] Further, in step S3, the mass ratio of the fermentation product, the nutritional fortifier, and the basic feed is (10 - 20):(1 - 5):100.

[0015] In summary, the present application has the following beneficial effects: In the technical solution of the present invention, through the fermentation of the composite strain, the complex organic matter in the culture medium is decomposed to generate nutritional components that are more easily absorbed and utilized by chickens, such as amino acids, vitamins, enzymes, etc., thereby improving the nutritional value of the feed. Thus, it promotes the absorption of protein by elderly laying hens and compensates for the problem of the decline in their digestive ability. The amino acid supply required for egg production is maintained through the fermentation system and the addition of nutritional fortifiers, which is used to maintain the size of the eggs and the quality of the egg white. The active substances generated during the fermentation process and components such as astragalus powder added have the effect of enhancing the immunity of chickens, helping chickens resist diseases, and improving the breeding efficiency. In addition, by adding nutritional fortifiers such as fish meal and amino acids, the proportion of nutritional components in the feed can be further optimized to meet the nutritional requirements of chickens during the growth process and promote the healthy growth of chickens.

[0016] In the technical solution of the present invention, a composite strain is obtained by compounding yeast, acetic acid bacteria, lactic acid bacteria, and Neurospora crassa, and is used for fermenting and preparing chicken feed. As a single-celled fungus, yeast can decompose organic matter and release organic calcium during metabolism. During the fermentation process, acetic acid bacteria can oxidize ethanol to produce acetic acid and simultaneously participate in the metabolism of other organic matters. After being compounded with yeast, acetic acid bacteria can assist yeast in decomposing inorganic calcium and promoting the generation of organic calcium. Neurospora crassa can better convert macronutrients such as cellulose, protein, and fat into sugars that are easily utilized by the strain. Lactic acid bacteria can ferment sugars to produce lactic acid in the strain compounding system. Its metabolic activity is beneficial to regulating the pH value of the fermentation system, providing guarantee for the growth and metabolism of yeast and other strains, promoting the stability of the composite strain system, and further promoting the conversion of inorganic calcium. In addition, Neurospora crassa can also increase active substances such as soluble peptides, organic acids, and carotenoids in the fermentation system, which is beneficial to improving the improvement effect of the feed on the production performance of elderly laying hens. Specific embodiments

[0017] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0018] The basal feed used in the following examples and comparative examples was obtained by mixing soybean meal and distillers' grains in a mass ratio of 3:1. The carbon source added to the culture medium was glucose; the inorganic calcium source used was eggshell powder.

[0019] The yeast used was provided by Shanghai Quanyan Biotechnology Co., Ltd., and the viable count was 1.24×10 11 cfu / g; the acetic acid bacteria used were provided by Wuhan Lanaibai Pharmaceutical and Chemical Co., Ltd., with the product number lnb-1693, and the viable count was 3×10 9 cfu / g; the lactic acid bacteria used were provided by Jiangsu Caiwei Biotechnology Co., Ltd., and the viable count was 1×10 10 cfu / g; Neurospora crassa was provided by the China Center for Industrial Culture Collection of Microorganisms, with the preservation number CICC 40203, and the viable count of the sample was 1×10 9 cfu / g.

[0020] Example 1 A nutrient-enriched chicken feed and its preparation method, including the following preparation steps: S1. Select a composite strain and co-activate it in an activation medium to obtain an activated strain. The specific operation is as follows: S11. Using MRS broth medium as the basal medium, add 2% carbon source, 0.01% magnesium sulfate, and 0.002% manganese sulfate to the basal medium by mass percentage, and adjust the pH value of the system to 5.0 to obtain the activation medium; S12. Inoculate the composite strain into the activation medium obtained in step S1 at an inoculation amount of 5%, set the culture temperature to 25°C, culture it with a shaker, the shaker speed is 100 rpm, and the culture time is 12 hours to obtain the activated strain; among them, the composite strain was obtained by mixing yeast, acetic acid bacteria, lactic acid bacteria, and Neurospora crassa in a viable count ratio of 1:0.2:0.1:0.5 in a sterile environment.

[0021] S2. Add the activated strain to the culture medium and ferment it. After fermentation, collect the fermentation product. The specific operation is as follows: S21. Prepare the culture medium according to the following formula: 5 wt% astragalus powder, 3 wt% medical stone, 50 wt% corn and corn cob mixture, 0.3 wt% magnesium sulfate, 6 wt% sodium dihydrogen phosphate, 6 wt% inorganic calcium source, and the rest is soybean powder; S22. Subsequently, the activated strains are mixed with the culture medium in a mass ratio of 1:8, 50% of the total weight of the two is added with water, and the pH value of the system is adjusted to 5.0. Then, the system is cultured by shaking in a shaker. Inorganic calcium sources are added at the 4th hour and the 6th hour of fermentation. The culture temperature is set at 25 °C, the shaker speed is 120 rpm, and after fermenting for 55 hours in a dark and ventilated place, the mixture including the culture medium is collected to obtain the fermentation product; wherein, the addition amount of the inorganic calcium source each time is 3 wt% of the culture medium.

[0022] S3. The fermentation product, nutritional enhancer and basic feed obtained in step S2 are mixed, pulverized and sieved through a 60-mesh sieve to obtain a nutritionally enhanced chicken feed; wherein, the nutritional enhancer is obtained by mixing 1 part of fish meal, 0.3 part of edible salt, 0.1 part of lysine, 0.1 part of methionine and 0.3 part of threonine; the mass ratio of the fermentation product, nutritional enhancer and basic feed is 10:1:100.

[0023] Example 2 A nutritionally enhanced chicken feed and its preparation method, including the following preparation steps: S1. Select a composite strain and co-activate it in an activation medium to obtain an activated strain. The specific operation is as follows: S11. Using MRS broth medium as the basic medium, by mass percentage, 3% of carbon source, 0.02% of magnesium sulfate and 0.003% of manganese sulfate are added to the basic medium, and the pH value of the system is adjusted to 5.5 to obtain the activation medium. S12. Inoculate the composite strain into the activation medium obtained in step S1 at an inoculation amount of 6%. Set the culture temperature at 28 °C, culture by shaking in a shaker, the shaker speed is 150 rpm, and the culture time is 18 hours to obtain the activated strain; wherein, the composite strain is obtained by mixing yeast, acetic acid bacteria, lactic acid bacteria and Neurospora crassa in a viable bacteria number ratio of 1:0.4:0.2:0.7 in a sterile environment.

[0024] S2. Add the activated strain to the culture medium and ferment and culture it. After the fermentation is completed, collect the fermentation product. The specific operation is as follows: S21. Prepare the culture medium according to the following formula: 7 wt% of astragalus powder, 4 wt% of medical stone, 55 wt% of corn and corn cob mixture, 0.4 wt% of magnesium sulfate, 8 wt% of sodium dihydrogen phosphate, 8 wt% of inorganic calcium source, and the rest is soybean powder. S22. Subsequently, the activated strains are mixed with the culture medium matrix at a mass ratio of 1:9, and 50% of the total weight of the two is added with water, and then the pH value of the system is adjusted to 5.5. Subsequently, the system is cultured by shaking in a shaker. Inorganic calcium sources are added at the 4th hour and the 6th hour of fermentation. The culture temperature is set at 28 °C, the shaker speed is 150 rpm, and after 65 hours of fermentation in a dark and ventilated place, the mixture including the culture medium matrix is collected to obtain the fermentation product; wherein, the addition amount of the inorganic calcium source each time is 4 wt% of the culture medium matrix.

[0025] S3. The fermentation product, the nutritional enhancer and the basic feed obtained in step S2 are mixed, pulverized and passed through an 80-mesh sieve to obtain a nutritionally enhanced chicken feed; wherein, the nutritional enhancer is obtained by mixing 2 parts of fish meal, 0.4 part of edible salt, 0.15 part of lysine, 0.15 part of methionine and 0.5 part of threonine; the mass ratio of the fermentation product, the nutritional enhancer and the basic feed is 15:3:100.

[0026] Example 3 A nutritionally enhanced chicken feed and its preparation method, including the following preparation steps: S1. Select a composite strain and co-activate it in an activation medium to obtain an activated strain. The specific operation is as follows: S11. Using MRS broth medium as the basic medium, by mass percentage, 3% of carbon source, 0.03% of magnesium sulfate, and 0.004% of manganese sulfate are added to the basic medium, and the pH value of the system is adjusted to 5.8 to obtain the activation medium. S12. Inoculate the composite strain into the activation medium obtained in step S1 at an inoculation amount of 7%, set the culture temperature at 30 °C, culture by shaking in a shaker, the shaker speed is 200 rpm, and the culture time is 24 hours to obtain the activated strain; wherein, the composite strain is obtained by mixing yeast, acetic acid bacteria, lactic acid bacteria and Neurospora crassa in a sterile environment at a viable bacteria ratio of 1:0.5:0.3:1.

[0027] S2. Add the activated strain to the culture medium matrix, ferment and culture, and collect the fermentation product after fermentation. The specific operation is as follows: S21. The culture medium matrix is prepared according to the following formula: 10 wt% of astragalus powder, 5 wt% of medical stone, 60 wt% of corn and corn cob mixture, 0.5 wt% of magnesium sulfate, 10 wt% of sodium dihydrogen phosphate, 10 wt% of inorganic calcium source, and the rest is soybean powder; S22. Subsequently, the activated strains are mixed with the culture medium matrix at a mass ratio of 1:10, and 50% of the total weight of the two is added with water, and then the pH value of the system is adjusted to 5.8. Subsequently, the system is cultured by shaking on a shaker. Inorganic calcium sources are added at the 4th hour and the 6th hour of fermentation. The culture temperature is set at 30 °C, the shaker speed is 150 rpm, and after fermenting for 75 hours in a dark and ventilated place, the mixture including the culture medium matrix is collected to obtain the fermentation product; wherein, the addition amount of the inorganic calcium source each time is 5 wt% of the activated culture medium.

[0028] S3. The fermentation product obtained in step S2, the nutritional enhancer and the basic feed are mixed, pulverized and passed through an 80-mesh sieve to obtain a nutritionally enhanced chicken feed; wherein, the nutritional enhancer is obtained by mixing 3 parts of fish meal, 0.5 part of edible salt, 0.2 part of lysine, 0.2 part of methionine and 0.6 part of threonine; the mass ratio of the fermentation product, the nutritional enhancer and the basic feed is 20:5:100.

[0029] Comparative Example 1 The difference between this comparative example and Example 1 is that in step S12, the composite strains are obtained by mixing yeast, lactic acid bacteria and Neurospora crassa in a viable cell number ratio of 1:0.3:0.5 in a sterile environment.

[0030] Comparative Example 2 The difference between this comparative example and Example 1 is that in step S12, the composite strains are obtained by mixing yeast, acetic acid bacteria and Neurospora crassa in a viable cell number ratio of 1:0.3:0.5 in a sterile environment.

[0031] Comparative Example 3 The difference between this comparative example and Example 1 is that in step S2, the inorganic calcium source is added all at once, and the specific operation is as follows: S21. The culture medium matrix is prepared according to the following formula: 5 wt% of astragalus powder, 3 wt% of medical stone, 50 wt% of corn and corn cob mixture, 0.3 wt% of magnesium sulfate, 6 wt% of sodium dihydrogen phosphate, 12 wt% of inorganic calcium source, and the rest is soybean powder; S22. Subsequently, the activated strains are mixed with the culture medium matrix at a mass ratio of 1:8, and 50% of the total weight of the two is added with water, and then the pH value of the system is adjusted to 5.0. Subsequently, the system is cultured by shaking on a shaker. The culture temperature is set at 25 °C, the shaker speed is 120 rpm, and after fermenting for 55 hours in a dark and ventilated place, the mixture including the culture medium matrix is collected to obtain the fermentation product.

[0032] Comparative Example 4 The difference between this comparative example and Example 1 is that the addition amount of the inorganic calcium source in the fermentation system is increased, and the specific operation is as follows: Culture medium prepared according to the following formula: 5 wt% of astragalus powder, 3 wt% of medical stone, 50 wt% of corn and corn cob mixture, 0.3 wt% of magnesium sulfate, 6 wt% of sodium dihydrogen phosphate, 10% of inorganic calcium source, and the rest is soybean powder; S22. Subsequently, the activated strain is mixed with the culture medium according to a mass ratio of 1:8, water accounting for 50% of the total weight of the two is added, and the pH value of the system is adjusted to 5.0. Then the system is cultured by shaking in a shaker. The inorganic calcium source is added at the 4th hour and the 6th hour of fermentation. The culture temperature is set at 25 °C, the shaker speed is 120 rpm, and after fermenting for 55 hours in a dark and ventilated place, the mixture including the culture medium is collected to obtain the fermentation product; among them, the addition amount of the inorganic calcium source each time is 10 wt% of the culture medium.

[0033] Performance test The feeding effects of the nutrient - fortified chicken feeds prepared in Examples 1 - 3 and Comparative Examples 1 - 4 of this application were tested.

[0034] Hy - Line Brown laying hens at the age of 500 ± 10 days were selected as the feeding objects, and they were fed with the chicken feeds prepared in Examples 1 - 3 and Comparative Examples 1 - 4 respectively.

[0035] The specific feeding method is as follows: Provide the laying hens with sufficient feed and clean drinking water to ensure their free access to food and water. Feed them regularly at 8 am and 6 pm. There should be a small amount of remaining feed in the trough after each feeding. Clean the chicken house every day to keep the house clean, hygienic, well - ventilated and dry. Disinfect the manure board every week, observe the mental state, feeding situation and feces state of the laying hens every day, and make records. Settle the remaining feed amount of the previous day every morning, accurately record the feed addition amount during the test period, and the daily feed waste amount, and calculate the average daily feed intake, egg production rate and average egg weight of the whole test period respectively. Among them, the average daily feed intake of laying hens = total feed consumption / (number of chickens × test days) × 100%; average egg production rate = total number of eggs produced / total number of laying hens × 100%; average egg weight = total weight of eggs produced / total number of eggs produced × 100%.

[0036] And evaluate the quality of the eggs produced by the laying hens in different groups, and measure the data of Haugh unit, eggshell strength and eggshell thickness in the eggs. Among them, Haugh unit = 100×lg [egg height + 7.57 - 1.7×(egg mass^0.37)]; the eggshell strength is measured with an eggshell strength tester.

[0037] The specific test results are shown in Table 1 below: Table 1

[0038] As can be seen from the results shown in Table 1 above: The comprehensive performance of the nutritionally fortified chicken feed prepared in Examples 1-3 of this application is significantly better than that of the products prepared in Comparative Examples 1-4. That is, within the scope of the technical solution defined in this application, the comprehensive performance of the prepared chicken feed product is excellent. During the statistical process, the number of broken eggs in the example group decreased significantly, the eggshell strength increased, the number of dirty eggs and deformed eggs decreased significantly, and the glossiness of the eggshell was significantly improved in terms of sensory perception compared to the eggs in the comparative examples.

[0039] As can be seen from the results in Comparative Examples 1 and 2, a composite strain obtained by compounding yeast, acetic acid bacteria, lactic acid bacteria, and Neurospora crassa is used for fermenting and preparing chicken feed. The interaction between the strains can promote the accumulation of active substances in the system and is beneficial to adjusting the content ratio of easily absorbable calcium, phosphorus, and other nutrient elements in the feed, improving the egg production promotion effect on elderly laying hens.

[0040] As can be seen from the results in Comparative Example 3, adding inorganic calcium raw materials in batches according to the characteristics of the strain fermentation process can better promote the accumulation of organic calcium components in the system and has a better promotion effect on the egg production quality of elderly laying hens.

[0041] As can be seen from the results in Comparative Example 4, only by using a reasonable formula mass of inorganic calcium source can the reasonable distribution of each nutrient element component in the feed be improved, and it has a better effect on improving the quality of the harvested eggs.

[0042] In the description of the specification, the description referring to terms such as "one embodiment", "example", "specific example", etc. means that the specific features, structures, materials, or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described can be combined in a suitable manner in any one or more embodiments or examples.

[0043] The above content is only an example and explanation of the present invention. Those skilled in the art of this technology can make various modifications or supplements to the described specific embodiments or use similar methods to replace them. As long as they do not deviate from the invention or exceed the scope defined by this claim book, they should all belong to the protection scope of the present invention.

Claims

1. A method for preparing a nutritionally fortified chicken feed, characterized in that: The method comprises the following preparation steps: S1. Selecting composite bacterial strains, and co-activating them in an activation medium to obtain activated bacterial strains; S2, adding the activated bacteria to the culture medium, fermenting and culturing, and collecting the fermentation product after the fermentation is completed; S3, mixing the fermentation product obtained in step S2, the nutritional enhancer and the basic feed, and crushing them through a 60-80 mesh sieve to obtain a nutritionally enhanced chicken feed; The composite bacterial species include yeast, acetic acid bacteria, lactic acid bacteria and Neurospora crassa.

2. The method for preparing a nutritionally fortified chicken feed according to claim 1, characterized in that: In step S1, the activated bacteria are prepared by the following steps: S11, using MRS broth as the basic medium, adding 2-3% carbon source, 0.01-0.03% magnesium sulfate, and 0.002-0.004% manganese sulfate to the basic medium by mass percentage, and adjusting the pH value of the system to 5.0-5.8 to obtain an activated medium; S12, inoculate the composite bacteria into the activation culture medium obtained in step S1 at an inoculum size of 5-7%, set the culture temperature to 25-30°C, culture on a shaker at a shaking rate of 100-200 rpm, and culture for 12-24 hours to obtain activated bacteria.

3. The method for preparing a nutritionally fortified chicken feed according to claim 2, characterized in that: In step S12, the composite bacteria are obtained by mixing yeast, acetic acid bacteria, lactic acid bacteria and Neurospora crassa in a sterile environment according to a live bacteria count ratio of 1:(0.2-0.5):(0.1-0.3):(0.5-1).

4. The method for preparing a nutritionally fortified chicken feed according to claim 2, characterized in that: In step S2, the activated bacteria are added to the culture medium for fermentation. After the fermentation is completed, the specific operation of collecting the fermentation product is as follows: S21, a culture matrix prepared according to the following formula: 5-10wt% astragalus powder, 3-5wt% medical stone, 50-60wt% corn and corncob mixture, 0.3-0.5wt% magnesium sulfate, 6-10wt% inorganic calcium source, 6-10wt% sodium dihydrogen phosphate, and the rest soybean powder; S22. The activated bacteria are then mixed with the culture matrix in a mass ratio of 1:(8-10), and 50% of the total weight of water is added to adjust the pH value of the system to 5.0-5.

8. The system is then shaken and cultured, and an inorganic calcium source is added at the 4th and 6th hours of fermentation. The culture temperature is set to 25-30°C and the shaking rate is set to 120-150rpm. After fermenting in a dark and ventilated place for 55-75 hours, the mixture contained in the culture medium matrix is ​​collected to obtain a fermentation product.

5. The method for preparing a nutritionally fortified chicken feed according to claim 4, characterized in that: In step S22, the amount of the inorganic calcium source added each time is 0.3-0.5 wt % of the culture matrix.

6. The method for preparing a nutritionally fortified chicken feed according to claim 2, characterized in that: In step S3, the nutritional enhancer is obtained by mixing 1-3 parts of fish meal, 0.3-0.5 parts of edible salt, 0.1-0.2 parts of lysine, 0.1-0.2 parts of methionine and 0.3-0.6 parts of threonine.

7. The method for preparing a nutritionally fortified chicken feed according to claim 2, characterized in that: In step S3, the basic feed is at least one of millet, distiller's grains, soybean meal, soybean powder, corn and wheat.

8. The method for preparing a nutritionally fortified chicken feed according to claim 2, characterized in that: In step S3, the mass ratio of the fermentation product, the nutritional enhancer and the basic feed is (10-20):(1-5):

100.

9. A chicken feed obtained by the method for preparing a nutritionally fortified chicken feed according to any one of claims 1 to 8.

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