Compound polysaccharide with weight-losing and lipid-lowering effects and application thereof

Abstract

The invention relates to the technical field of food science and technology, in particular to compound polysaccharide with weight losing and lipid lowering effects and application, and the compound polysaccharide is formed by directly mixing and compounding corn filament polysaccharide and kudzuvine root polysaccharide; the mass ratio of the corn filament polysaccharide to the radix puerariae polysaccharide is 1: (1-3); the extraction method of the corn filament polysaccharide comprises the following steps: soaking corn filaments in an organic solvent for degreasing, crushing, adding cellulase and papain, heating and refluxing, and concentrating to obtain the corn filament polysaccharide. The corn filament polysaccharide prepared by a special process is compounded with the kudzuvine root polysaccharide, so that the weight-losing and lipid-lowering effects are greatly improved, and a new technical scheme is provided for developing efficient and safe natural weight-losing and lipid-lowering products.

Description

Technical Field

[0001] The present invention relates to the technical field of food science and technology, and particularly relates to a composite polysaccharide with weight loss and lipid-lowering effects and its application. Background Art

[0002] Obesity is a complex metabolic disease characterized by weight gain, dyslipidemia, metabolic disorders or oxidative stress. Long-term intake of a high-fat diet can lead to the accumulation of adipose tissue, resulting in weight gain and the rupture of cells within adipose tissue, thereby inducing inflammation in the body. Currently, a large number of studies have shown that cardiovascular diseases such as diabetes and hypertension are closely related to obesity. Therefore, preventing or reducing obesity is an important factor in maintaining a healthy body.

[0003] Corn silk is the dried style and stigma of corn, and is a traditional Chinese herbal medicine and functional food raw material in China. Folk often use hot water to soak corn silk to delay the rise of blood sugar. Corn silk contains various functional active substances such as polysaccharides, flavonoids and saponins. Studies have shown that corn silk has multiple effects such as hypoglycemic, hypouricemic and antibacterial and anti-inflammatory effects, and is safe and non-toxic. It is a natural raw material for developing weight loss and lipid-lowering foods. Although corn silk polysaccharide has potential biological activity, the single use of corn silk polysaccharide has problems such as a narrow action target and low absorption efficiency, resulting in limited weight loss and lipid-lowering effects. Summary of the Invention

[0004] To solve the above problems, the present invention provides a composite polysaccharide with weight loss and lipid-lowering effects and its application.

[0005] The present invention is achieved by the following technical solutions: A composite polysaccharide with weight loss and lipid-lowering effects, characterized in that the composite polysaccharide is directly mixed and compounded by corn silk polysaccharide and kudzu root polysaccharide; the mass ratio of the corn silk polysaccharide to the kudzu root polysaccharide is 1:1 to 3.

[0006] The extraction method of the corn silk polysaccharide is: soaking corn silk with an organic solvent for defatting, adding cellulase and papain after crushing, heating under reflux, and concentrating to obtain corn silk polysaccharide.

[0007] Preferably, the extraction method of the corn silk polysaccharide specifically includes the following steps: (1) Drying corn silk at 35°C to 45°C until constant weight, pulverizing and sieving through a 70-mesh to 90-mesh sieve; soaking the sieved corn silk with 3 to 5 times the volume of an organic solvent for defatting, and air-drying to constant weight after defatting to obtain corn silk powder.

[0008] (2)Weigh the corn silk powder obtained in (1), add 30 to 50 times the amount of distilled water, and then perform a crushing treatment to obtain a corn silk mixture.

[0009] (3)Add cellulase and papain to the corn silk mixture obtained in (2) respectively, perform a heat reflux treatment, and centrifuge to collect the supernatant; the addition amount of the cellulase accounts for 2% - 4% of the weight of the corn silk; the addition amount of the papain accounts for 2% - 4% of the weight of the corn silk.

[0010] (4)Concentrate the supernatant obtained in (3) at 60°C - 70°C, then add ethanol with a volume 3 to 4 times that of the concentrated solution to precipitate polysaccharides, let it stand for 12h - 18h, centrifuge, remove the supernatant, and freeze-dry to obtain corn silk polysaccharides.

[0011] Preferably, the soaking time of the organic solvent is 6h - 12h.

[0012] Preferably, the organic solvent used is petroleum ether.

[0013] Preferably, the crushing treatment is ultrasonic crushing treatment, the power of the ultrasonic crushing treatment is 200W - 500W, the frequency is 20kHz - 40kHz, and the time is 10min - 30min.

[0014] Preferably, the enzyme activity of the cellulase is 40u / mg - 60u / mg; the enzyme activity of the papain is 700u / mg - 900u / mg.

[0015] Preferably, the ethanol is anhydrous ethanol.

[0016] Preferably, the temperature of the heat reflux treatment is 60°C - 70°C; the time is 2h - 3h.

[0017] Application of the compound polysaccharide with weight loss and lipid-lowering effects in the preparation of weight loss and lipid-lowering foods.

[0018] Compared with the prior art, the present invention has the following beneficial effects: The present invention provides a composite polysaccharide with weight loss and lipid-lowering effects. The composite polysaccharide is directly mixed and compounded from corn silk polysaccharide and kudzu root polysaccharide; the mass ratio of the corn silk polysaccharide to the kudzu root polysaccharide is 1:1 to 3; the extraction method of the corn silk polysaccharide is as follows: soaking corn silk in an organic solvent for defatting, adding cellulase and papain after crushing treatment, heating under reflux, and concentrating to obtain corn silk polysaccharide. The corn silk polysaccharide prepared by a special process for the first time in the present invention is compounded with kudzu root polysaccharide. The experimental results show that the composite polysaccharide can reduce the body weight of obese mice by 8.04 g to 13.36 g, the liver weight by 0.27 g to 0.43 g, and the epididymal fat weight by 0.45 g to 0.99 g. The composite polysaccharide can reduce the serum total cholesterol content of obese mice by 1.75 mmol / mL to 2.29 mmol / mL and the triglyceride content by 0.9 mmol / mL to 1.14 mmol / mL. Through the pathological observation results of mouse liver tissue, it is found that the composite polysaccharide can reduce the fat in the cytoplasm of liver tissue cells of mice and alleviate cell lesions, indicating that the compounding of the corn silk polysaccharide and kudzu root polysaccharide prepared by a special process in the present invention greatly improves the weight loss and lipid-lowering effects, providing a new technical solution for the development of efficient and safe natural weight loss and lipid-lowering products. In addition, both the corn silk polysaccharide and kudzu root polysaccharide used in the compounding of the present invention are naturally extracted substances, which are safer than chemical drugs. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. Obviously, the following drawings are only some embodiments of the present invention. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.

[0020] Figure 1 It is the pathological observation results of the liver tissues of each group of mice after being treated with the compound of corn silk polysaccharide and kudzu root polysaccharide of the present invention; among them, A is the blank control group, B is the orlistat positive control group, C is the model group, D is the corn silk polysaccharide CSP group, E is the kudzu root polysaccharide PLP group, and F is the composite polysaccharide CSP-PLP group. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0021] To facilitate the understanding of the present invention, the present invention will be described more comprehensively below, and preferred embodiments of the present invention are given. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the understanding of the disclosure of the present invention more thorough and comprehensive.

[0022] Unless otherwise defined, all technical and scientific terms used in this invention have the same meaning as commonly understood by those skilled in the technical field to which this invention belongs. The terms used in the description of this invention are only for the purpose of describing specific embodiments and are not intended to limit this invention.

[0023] The inventive concept of this invention is as follows: Corn silk is the dried style and stigma of corn, and is a traditional Chinese herbal medicine and raw material for functional foods in China. Folk often use hot water to soak corn silk to delay the rise of blood sugar. Corn silk contains various functional active substances, such as polysaccharides, flavonoids, and saponins. Research shows that corn silk has various effects such as lowering blood sugar, lowering uric acid, and antibacterial and anti-inflammatory effects, and is safe and non-toxic, and is a natural raw material for developing weight loss and lipid-lowering foods. Although corn silk polysaccharide has potential biological activity, the single use of corn silk polysaccharide has problems such as a narrow action target and low absorption efficiency, resulting in limited weight loss and lipid-lowering effects.

[0024] Based on this, the present invention provides a composite polysaccharide with significant weight loss and lipid-lowering effects. First, the present invention has developed a special extraction process for corn silk polysaccharide: using low-temperature drying to protect heat-sensitive components, soaking with petroleum ether for fractional degreasing to remove impurities, combining the synergistic hydrolysis of cellulase and papain and ultrasonic-assisted fragmentation technology, and increasing the yield to 8.0% - 8.2% while ensuring the integrity of the polysaccharide structure, and obtaining a highly active polysaccharide with a uniform molecular weight and a weight-average molecular weight of 10.257 kDa. This process effectively retains more active components related to weight loss and lipid-lowering in the polysaccharide, such as β-glycosidic bonds and hydroxyl groups. On this basis, the corn silk polysaccharide prepared by the special process of the present invention is compounded with pueraria polysaccharide, which has a significant synergistic effect, can reduce the body weight of obese mice by 8.04 - 13.36 g, reduce the epididymal fat by 0.45 - 0.99 g, reduce the serum TC by 1.75 mmol / L - 2.29 mmol / L, and reduce the TG by 0.9 mmol / L - 1.14 mmol / L, greatly improving the weight loss and lipid-lowering effects. The weight loss and lipid-lowering effects achieved by compounding the corn silk polysaccharide prepared by the special process of the present invention with pueraria polysaccharide cannot be achieved by the prior art, providing a new technical solution for the development of high-efficiency and safe natural weight loss and lipid-lowering products.

[0025] The beneficial effects of the present invention are illustrated below through specific examples.

[0026] Pueraria polysaccharide was purchased from Shaanxi Xinkang Biotechnology Co., Ltd.

[0027] Cellulase was purchased from Shanghai Yuanye Biotechnology Co., Ltd.

[0028] Papain was purchased from Shanghai Yuanye Biotechnology Co., Ltd.

[0029] Example 1 A composite polysaccharide with weight loss and lipid-lowering effects.

[0030] The composite polysaccharide is composed of corn silk polysaccharide and kudzu root polysaccharide; the mass ratio of the corn silk polysaccharide to the kudzu root polysaccharide is 1:1.

[0031] Example 2 A composite polysaccharide with weight loss and lipid-lowering effects.

[0032] The composite polysaccharide is composed of corn silk polysaccharide and kudzu root polysaccharide; the mass ratio of the corn silk polysaccharide to the kudzu root polysaccharide is 1:2.

[0033] Example 3 A composite polysaccharide with weight loss and lipid-lowering effects.

[0034] The composite polysaccharide is composed of corn silk polysaccharide and kudzu root polysaccharide; the mass ratio of the corn silk polysaccharide to the kudzu root polysaccharide is 1:3.

[0035] Example 4 The corn silk polysaccharide is obtained by the following method.

[0036] (1) Put the corn silk into an oven and dry it at 35 °C to constant weight. Use a grinder to crush the corn silk and pass it through a 70-mesh sieve; soak the sieved corn silk with 3 times its volume of petroleum ether for 6 h, repeat three times to ensure complete defatting, and air-dry it to constant weight after defatting to obtain corn silk powder.

[0037] (2) Weigh the corn silk powder obtained in (1), add 30 times the amount of distilled water, and put it into a cell disruptor for ultrasonic disruption treatment. The power of the ultrasonic disruption treatment is 200 W, the frequency is 20 kHz, and the time is 10 min.

[0038] (3) Add cellulase and papain to the corn silk mixture obtained in (2), heat and reflux at 60 °C for 2 h, and centrifuge to collect the supernatant; the addition amount of the cellulase accounts for 2% of the weight of the corn silk; the addition amount of the papain accounts for 2% of the weight of the corn silk. The enzyme activity of the cellulase is 40 u / mg; the enzyme activity of the papain is 700 u / mg.

[0039] (4) Concentrate the supernatant obtained in (3) to 1 / 10 of its original volume at 60 °C, add 3 times its volume of absolute ethanol, stir evenly, let it stand at 4 °C for 12 h, centrifuge, remove the supernatant, and freeze-dry to obtain corn silk polysaccharide.

[0040] (5) The polysaccharide from corn silk was purified by the Sevage method four times for protein removal, freeze-dried, the freeze-dried powder was redissolved in water, and dialysis was carried out to obtain the purified polysaccharide from corn silk.

[0041] Example 5 The polysaccharide from corn silk was obtained by the following method.

[0042] (1) The corn silk was placed in an oven and dried to a constant weight at 35°C. The corn silk was crushed using a grinder and passed through a 70-mesh sieve. The sieved corn silk was soaked in 3 times its volume of petroleum ether for 6 h, and the process was repeated three times to ensure complete defatting. After defatting, it was air-dried to a constant weight to obtain corn silk powder.

[0043] (2) Weigh the corn silk powder obtained in (1), add 30 times the volume of distilled water, and place it in a cell disruptor for ultrasonic disruption. The power of the ultrasonic disruption was 300 W, the frequency was 30 kHz, and the time was 20 min.

[0044] (3) Add cellulase and papain to the corn silk mixture obtained in (2), heat under reflux at 60°C for 2 h, and centrifuge to collect the supernatant. The addition amount of the cellulase accounted for 3% of the weight of the corn silk; the addition amount of the papain accounted for 3% of the weight of the corn silk. The enzyme activity of the cellulase was 50 u / mg; the enzyme activity of the papain was 800 u / mg.

[0045] (4) The supernatant obtained in (3) was concentrated to 1 / 10 of its original volume at 60°C, 3 times its volume of absolute ethanol was added, after stirring evenly, it was left standing at 4°C for 12 h, centrifuged, the supernatant was removed, and freeze-dried to obtain the polysaccharide from corn silk.

[0046] (5) The polysaccharide from corn silk was purified by the Sevage method four times for protein removal, freeze-dried, the freeze-dried powder was redissolved in water, and dialysis was carried out to obtain the purified polysaccharide from corn silk.

[0047] Example 6 The polysaccharide from corn silk was obtained by the following method.

[0048] (1) The corn silk was placed in an oven and dried to a constant weight at 35°C. The corn silk was crushed using a grinder and passed through a 70-mesh sieve. The sieved corn silk was soaked in 3 times its volume of petroleum ether for 6 h, and the process was repeated three times to ensure complete defatting. After defatting, it was air-dried to a constant weight to obtain corn silk powder.

[0049] (2) Weigh the corn silk powder obtained in (1), add 30 times the volume of distilled water, and place it in a cell disruptor for ultrasonic disruption. The power of the ultrasonic disruption was 500 W, the frequency was 40 kHz, and the time was 30 min.

[0050] (3) Add cellulase and papain to the corn silk mixture obtained in (2), heat under reflux at 60 °C for 2 h, and centrifuge to collect the supernatant; the addition amount of the cellulase accounts for 4% of the weight of the corn silk; the addition amount of the papain accounts for 4% of the weight of the corn silk. The enzyme activity of the cellulase is 60 u / mg; the enzyme activity of the papain is 900 u / mg.

[0051] (4) Concentrate the supernatant obtained in (3) to 1 / 10 of the original volume at 60 °C, add 3 times the volume of absolute ethanol, stir evenly, let stand at 4 °C for 12 h, centrifuge, remove the supernatant, and freeze-dry to obtain corn silk polysaccharide.

[0052] (5) Purify the corn silk polysaccharide by deproteinizing it 4 times with the Sevage method, freeze-dry, dissolve the freeze-dried powder in water, and dialyze to obtain the purified corn silk polysaccharide.

[0053] Example 7 The corn silk polysaccharide is obtained by the following method.

[0054] (1) Put the corn silk into an oven, dry it to constant weight at 40 °C, crush the corn silk with a grinder, and pass through an 80-mesh sieve; soak the sieved corn silk with 4 times the volume of petroleum ether for 10 h, repeat three times to ensure complete defatting, and air-dry to constant weight after defatting to obtain corn silk powder.

[0055] (2) Weigh the corn silk powder obtained in (1), add 40 times the volume of distilled water, put it into a cell disruptor for ultrasonic disruption treatment, the power of the ultrasonic disruption treatment is 200 W, the frequency is 20 kHz, and the time is 10 min.

[0056] (3) Add cellulase and papain to the corn silk mixture obtained in (2), heat under reflux at 65 °C for 2.5 h, and centrifuge to collect the supernatant; the addition amount of the cellulase accounts for 2% of the weight of the corn silk; the addition amount of the papain accounts for 2% of the weight of the corn silk. The enzyme activity of the cellulase is 40 u / mg; the enzyme activity of the papain is 700 u / mg.

[0057] (4) Concentrate the supernatant obtained in (3) to 1 / 10 of the original volume at 65 °C, add 4 times the volume of absolute ethanol, stir evenly, let stand at 4 °C for 15 h, centrifuge, remove the supernatant, and freeze-dry to obtain corn silk polysaccharide.

[0058] (5) Purify the corn silk polysaccharide by deproteinizing it 4 times with the Sevage method, freeze-dry, dissolve the freeze-dried powder in water, and dialyze to obtain the purified corn silk polysaccharide.

[0059] Example 8 The polysaccharide from corn silk is obtained by the following method.

[0060] (1) Put the corn silk into an oven and dry it at 40 °C until constant weight. Use a grinder to crush the corn silk and sieve it through an 80-mesh sieve. Soak the sieved corn silk in petroleum ether with a volume 4 times that of the corn silk for 10 h, repeat three times to ensure complete defatting, and after defatting, air-dry it to constant weight to obtain corn silk powder.

[0061] (2) Weigh the corn silk powder obtained in (1), add distilled water with a volume 40 times that of the corn silk powder, put it into a cell disruptor for ultrasonic disruption treatment. The power of the ultrasonic disruption treatment is 300 W, the frequency is 30 kHz, and the time is 20 min.

[0062] (3) Add cellulase and papain to the corn silk mixture obtained in (2) respectively, heat and reflux at 65 °C for 2.5 h, and centrifuge to collect the supernatant. The addition amount of the cellulase accounts for 3% of the weight of the corn silk; the addition amount of the papain accounts for 3% of the weight of the corn silk. The enzyme activity of the cellulase is 50 u / mg; the enzyme activity of the papain is 800 u / mg.

[0063] (4) Concentrate the supernatant obtained in (3) to 1 / 10 of the original volume at 65 °C, add anhydrous ethanol with a volume 4 times that of the supernatant, stir evenly, then let it stand at 4 °C for 15 h, centrifuge, remove the supernatant, and freeze-dry to obtain the polysaccharide from corn silk.

[0064] (5) Purify the polysaccharide from corn silk by the Sevage method for 4 times, freeze-dry it, dissolve the freeze-dried powder in water, and dialyze to obtain the purified polysaccharide from corn silk.

[0065] Example 9 The polysaccharide from corn silk is obtained by the following method.

[0066] (1) Put the corn silk into an oven and dry it at 40 °C until constant weight. Use a grinder to crush the corn silk and sieve it through an 80-mesh sieve. Soak the sieved corn silk in petroleum ether with a volume 4 times that of the corn silk for 10 h, repeat three times to ensure complete defatting, and after defatting, air-dry it to constant weight to obtain corn silk powder.

[0067] (2) Weigh the corn silk powder obtained in (1), add distilled water with a volume 40 times that of the corn silk powder, put it into a cell disruptor for ultrasonic disruption treatment. The power of the ultrasonic disruption treatment is 500 W, the frequency is 40 kHz, and the time is 30 min.

[0068] (3) Add cellulase and papain to the corn silk mixture obtained in (2), heat under reflux at 65 °C for 2.5 h, and centrifuge to collect the supernatant; the addition amount of the cellulase accounts for 4% of the weight of the corn silk; the addition amount of the papain accounts for 4% of the weight of the corn silk. The enzyme activity of the cellulase is 60 u / mg; the enzyme activity of the papain is 900 u / mg.

[0069] (4) Concentrate the supernatant obtained in (3) to 1 / 10 of the original volume at 65 °C, add 4 times the volume of absolute ethanol, stir evenly, let it stand at 4 °C for 15 h, centrifuge, remove the supernatant, and freeze-dry to obtain corn silk polysaccharide.

[0070] (5) Purify the corn silk polysaccharide by deproteinizing it 4 times with the Sevage method, freeze-dry, dissolve the freeze-dried powder in water, and dialyze to obtain the purified corn silk polysaccharide.

[0071] Example 10 The corn silk polysaccharide is obtained by the following method.

[0072] (1) Put the corn silk into an oven and dry it to constant weight at 45 °C. Use a grinder to crush the corn silk and pass it through a 90-mesh sieve; soak the sieved corn silk with 5 times the volume of petroleum ether for 12 h, repeat three times to ensure complete defatting, and air-dry to constant weight after defatting to obtain corn silk powder.

[0073] (2) Weigh the corn silk powder obtained in (1), add 50 times the volume of distilled water, put it into a cell disruptor for ultrasonic disruption treatment. The power of the ultrasonic disruption treatment is 200 W, the frequency is 20 kHz, and the time is 10 min.

[0074] (3) Add cellulase and papain to the corn silk mixture obtained in (2), heat under reflux at 70 °C for 3 h, and centrifuge to collect the supernatant; the addition amount of the cellulase accounts for 2% of the weight of the corn silk; the addition amount of the papain accounts for 2% of the weight of the corn silk. The enzyme activity of the cellulase is 40 u / mg; the enzyme activity of the papain is 700 u / mg.

[0075] (4) Concentrate the supernatant obtained in (3) to 1 / 10 of the original volume at 70 °C, add 4 times the volume of absolute ethanol, stir evenly, let it stand at 4 °C for 18 h, centrifuge, remove the supernatant, and freeze-dry to obtain corn silk polysaccharide.

[0076] (5) Purify the corn silk polysaccharide by deproteinizing it 4 times with the Sevage method, freeze-dry, dissolve the freeze-dried powder in water, and dialyze to obtain the purified corn silk polysaccharide.

[0077] Example 11 The polysaccharide from corn silk is obtained by the following method.

[0078] (1) Put the corn silk into an oven and dry it at 45 °C until constant weight. Use a grinder to crush the corn silk and sieve it through a 90-mesh sieve. Soak the sieved corn silk in 5 times its volume of petroleum ether for 12 h, repeat three times to ensure complete defatting, and after defatting, air-dry it to constant weight to obtain corn silk powder.

[0079] (2) Weigh the corn silk powder obtained in (1), add 50 times the volume of distilled water, put it into a cell disruptor for ultrasonic disruption treatment. The power of the ultrasonic disruption treatment is 300 W, the frequency is 30 kHz, and the time is 20 min.

[0080] (3) Add cellulase and papain to the corn silk mixture obtained in (2), heat and reflux at 70 °C for 3 h, and centrifuge to collect the supernatant. The addition amount of the cellulase accounts for 3% of the weight of the corn silk; the addition amount of the papain accounts for 3% of the weight of the corn silk. The enzyme activity of the cellulase is 50 u / mg; the enzyme activity of the papain is 800 u / mg.

[0081] (4) Concentrate the supernatant obtained in (3) to 1 / 10 of its original volume at 70 °C, add 4 times the volume of absolute ethanol, stir evenly, then let it stand at 4 °C for 18 h, centrifuge, remove the supernatant, and freeze-dry to obtain the polysaccharide from corn silk.

[0082] (5) Purify the polysaccharide from corn silk by the Sevage method to remove protein 4 times, freeze-dry it, dissolve the freeze-dried powder in water, and dialyze to obtain the purified polysaccharide from corn silk.

[0083] Example 12 The polysaccharide from corn silk is obtained by the following method.

[0084] (1) Put the corn silk into an oven and dry it at 45 °C until constant weight. Use a grinder to crush the corn silk and sieve it through a 90-mesh sieve. Soak the sieved corn silk in 5 times its volume of petroleum ether for 12 h, repeat three times to ensure complete defatting, and after defatting, air-dry it to constant weight to obtain corn silk powder.

[0085] (2) Weigh the corn silk powder obtained in (1), add 50 times the volume of distilled water, put it into a cell disruptor for ultrasonic disruption treatment. The power of the ultrasonic disruption treatment is 500 W, the frequency is 40 kHz, and the time is 30 min.

[0086] (3) Add cellulase and papain to the corn silk mixture obtained in (2), heat under reflux at 70 °C for 3 h, and centrifuge to collect the supernatant; the addition amount of the cellulase accounts for 4% of the weight of the corn silk; the addition amount of the papain accounts for 4% of the weight of the corn silk. The enzyme activity of the cellulase is 60 u / mg; the enzyme activity of the papain is 900 u / mg.

[0087] (4) Concentrate the supernatant obtained in (3) to 1 / 10 of the original volume at 70 °C, add 4 times the volume of absolute ethanol, stir evenly, let stand at 4 °C for 18 h, centrifuge, remove the supernatant, and freeze-dry to obtain corn silk polysaccharide.

[0088] (5) Purify the corn silk polysaccharide by Sevage method for deproteinization 4 times, freeze-dry, dissolve the freeze-dried powder in water, and dialyze to obtain the purified corn silk polysaccharide.

[0089] Experimental Example 1: Detection of the yield and weight-average molecular weight of corn silk polysaccharide 1. Prepare a glucose standard solution with a concentration of 0.1 mg / mL by dissolving a glucose standard product in distilled water. Respectively take 0 mL, 0.1 mL, 0.2 mL, 0.4 mL, 0.6 mL, 0.8 mL, and 1.0 mL of the glucose standard solution and add them into colorimetric tubes. Add distilled water to make the total volume of each tube reach 1 mL. Then add 1 mL of a phenol solution with a volume fraction of 5% to each tube. After shaking well, quickly add 5 mL of concentrated sulfuric acid with a pipette. After standing and reacting for 30 min, use an ultraviolet spectrometer to measure the absorbance of each tube at 490 nm respectively, and draw a glucose standard curve with the regression equation of y = 10.656x - 0.0002, R 2 = 0.9995, showing a good linear relationship.

[0090] Calculate the yield of the corn silk polysaccharide obtained in Examples 4 to 12 through the following formula, and the final yields are all between 8.0% and 8.2%.

[0091] ; In the formula: Y is the yield of corn silk polysaccharide, %; C is the concentration of corn silk polysaccharide, mg / mL; N is the dilution factor; V is the total volume of the solution, mL; m is the mass of the raw material powder, g.

[0092] 2. Detect that the weight-average molecular weight of the corn silk polysaccharide is 10.257 kDa.

[0093] Experimental Example 1: In vivo experiment The high-fat diet formula is: 20.70% casein, 60% glucose, 7.77% cellulose, 5% lard, 6.53% basal diet.

[0094] Grouping, model establishment, and drug administration: Thirty-six male C57 / 6J mice were fasted for 12 h with free access to water before the experiment. The mice were randomly divided into 6 groups of 6 mice each: blank control group, model group, orlistat positive control group with an orlistat dosage of 30 mg / kg; corn silk polysaccharide CSP group with a corn silk polysaccharide dosage of 300 mg / kg; pueraria polysaccharide PLP group with a pueraria polysaccharide dosage of 300 mg / kg; composite polysaccharide CSP-PLP group, which was a composite polysaccharide prepared from the corn silk polysaccharide prepared in Example 8 and pueraria polysaccharide in a mass ratio of 1:1, with a dosage of 300 mg / kg. Except for the blank control group, the other groups were given a high-fat diet. During the experiment, the animals had free access to water and food. When the average body weight of all groups exceeded 30% of the body weight of the blank control group, the model was successfully established and drug administration began. The blank control group and the model group were given an equal amount of normal saline for 6 consecutive weeks. The body weight was measured 3 times a week, and the drug dosage was adjusted according to the body weight.

[0095] Sample collection: At the end of the 12th week, the mice in each group were fasted for 12 h, weighed, and then sacrificed. Samples such as blood, liver, and epididymal fat were collected. After the blood was allowed to stand for 2 h, it was centrifuged at 3500 rpm for 10 min at 4°C, and then the serum was stored at -80°C for later use.

[0096] Index detection: Weigh the liver and epididymal fat. Use the corresponding kits to detect the serum TC and TG levels. And perform pathological observations on the liver tissues of the mice.

[0097] Statistical method: The data were all processed using SPSS 28.0 statistical software, and the graphs were made using Origin 2021 and Graphpad Pism9.5 software.

[0098] The results showed that compared with the model group, the composite polysaccharide could reduce the body weight of obese mice by 8.04 g - 13.36 g, the liver weight by 0.27 g - 0.43 g, and the epididymal fat weight by 0.45 g - 0.99 g; the composite polysaccharide could reduce the serum total cholesterol, that is, the TC content, by 1.75 mmol / mL - 2.29 mmol / mL and the triglyceride, that is, the TG content, by 0.9 mmol / mL - 1.14 mmol / mL in obese mice. Through the pathological observation results of the mouse liver tissues, it was found that the composite polysaccharide could reduce the fat in the cytoplasm of liver tissue cells and alleviate cell lesions, as Figure 1 shown.

[0099] Taking the polysaccharides from corn silk prepared in Examples 4 to 12 respectively as raw materials, and conducting the same in-vivo experiments as above on the composite polysaccharides prepared by compounding with pueraria polysaccharides at mass ratios of 1:1, 1:2, and 1:3, all can achieve the effect of reducing the body weight, liver weight, epididymal fat weight, total cholesterol content, and triglyceride content of obese mice.

[0100] The technical features of the above-described embodiments can be combined arbitrarily. For the sake of brevity of description, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, it should be considered as the scope described in this specification.

[0101] The above-described embodiments merely represent several implementation manners of the present invention. Their descriptions are relatively specific and detailed, but they should not be construed as limiting the scope of the invention patent. For those of ordinary skill in the art, without departing from the concept of the present invention, several modifications and improvements can still be made, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the invention patent should be subject to the appended claims.

Claims

1. A composite polysaccharide having weight loss and lipid-lowering effects, characterized in that: The composite polysaccharide is prepared by directly mixing corn silk polysaccharide and kudzu root polysaccharide; the mass ratio of corn silk polysaccharide to kudzu root polysaccharide is 1:1-3; The method for extracting corn silk polysaccharide comprises: soaking corn silk in an organic solvent to defatted, adding cellulase and papain after crushing, heating and refluxing, and concentrating to obtain corn silk polysaccharide.

2. The complex polysaccharide with weight loss and lipid-lowering effects according to claim 1, characterized in that: The method for extracting corn silk polysaccharide specifically comprises the following steps: (1) drying corn silk at 35°C to 45°C to constant weight, crushing and passing through a 70-mesh to 90-mesh sieve; soaking and defatting the sieved corn silk in 3-5 times the volume of an organic solvent, defatting and air-drying to constant weight to obtain corn silk powder; (2) Weighing the corn silk powder obtained in (1), adding 30 to 50 times of distilled water, and crushing the powder to obtain a corn silk mixed liquid; (3) adding cellulase and papain to the corn silk mixture obtained in (2), heating and refluxing, and collecting the supernatant by centrifugation; the amount of the cellulase added is 2% to 4% of the weight of the corn silk; the amount of the papain added is 2% to 4% of the weight of the corn silk; (4) Concentrate the supernatant obtained in (3) at 60°C to 70°C, add ethanol in a volume equivalent to 3 to 4 times the volume of the concentrate, let stand for 12 h to 18 h, centrifuge, remove the supernatant, and lyophilize to obtain corn silk polysaccharide.

3. The complex polysaccharide with weight loss and lipid-lowering effects as claimed in claim 2, characterized in that: The organic solvent soaking time is 6h~12h.

4. The complex polysaccharide with weight loss and lipid-lowering effects as claimed in claim 2, characterized in that: The organic solvent used is petroleum ether.

5. The complex polysaccharide with weight loss and lipid-lowering effects as claimed in claim 2, characterized in that: The crushing treatment is ultrasonic crushing treatment, the power of the ultrasonic crushing treatment is 200W~500W, the frequency is 20kHz~40kHz, and the time is 10min~30min.

6. The complex polysaccharide with weight loss and lipid-lowering effects as claimed in claim 2, characterized in that: The enzymatic activity of the cellulase is 40u / mg-60u / mg; the enzymatic activity of the papain is 700u / mg-900u / mg.

7. The complex polysaccharide with weight loss and lipid-lowering effects as claimed in claim 2, characterized in that: The ethanol is anhydrous ethanol.

8. The complex polysaccharide with weight loss and lipid-lowering effects as claimed in claim 2, characterized in that: The temperature of the heating reflux treatment is 60° C. to 70° C. and the time is 2 h to 3 h.

9. Use of the complex polysaccharide with weight loss and lipid-lowering effects as claimed in claim 1 in the preparation of weight loss and lipid-lowering foods.

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