Washing and care composition, washing and care product and application

Abstract

The invention provides a washing and care composition, a washing and care product and application, the washing and care composition comprises sophorolipid, a first surfactant and a second surfactant, the first surfactant comprises an anionic surfactant, and the second surfactant comprises a zwitterionic surfactant and / or a nonionic surfactant; the mass ratio of the sophorolipid to the first surfactant is 1: (1-10), and the mass ratio of the sophorolipid to the second surfactant is 1: (1-10). The sophorolipid has the capability of directly and quickly removing endotoxin, and the sophorolipid and the surfactant are compounded according to a specific ratio to play a synergistic role, so that the endotoxin removing efficiency is remarkably improved, and the occurrence of skin irritation and inflammatory response caused by the endotoxin when a skin barrier is weak or damaged can be remarkably reduced.

Description

Technical Field

[0001] The present application relates to the technical field of daily chemical products, and particularly to a washing and care composition, a washing and care product and an application thereof. Background Art

[0002] Endotoxin is a lipopolysaccharide in the cell wall of Gram-negative bacteria and usually does not cause problems by being absorbed through the skin. However, when the skin is damaged, suffering from skin diseases, or when certain drugs that weaken the skin barrier such as corticosteroids are used for a long time, environmental exogenous endotoxins may penetrate through the damaged skin, causing irritation or allergic reactions, and even causing serious adverse reactions such as fever, microcirculation disorders, endotoxin shock and disseminated intravascular coagulation. However, there has not been the development of a cleaning product that can effectively remove endotoxin yet. Therefore, there is an urgent need to develop a personal cleaning product that can effectively remove endotoxin. Summary of the Invention

[0003] Based on this, the present application provides a washing and care composition, a washing and care product and an application thereof, so as to significantly reduce the occurrence of skin irritation and inflammatory reactions caused by endotoxin when the skin barrier is weak or damaged.

[0004] In the first aspect of the present application, a washing and care composition is provided, which includes sophorolipid, a first surfactant and a second surfactant. The first surfactant includes an anionic surfactant, and the second surfactant includes an amphoteric ion surfactant and / or a non-ionic surfactant; the mass ratio of the sophorolipid to the first surfactant is 1:(1 - 10), and the mass ratio of the sophorolipid to the second surfactant is 1:(1 - 10).

[0005] In some embodiments, the anionic surfactant includes one or more of sodium cocoyl glutamate and potassium cocoyl glycinate; and / or

[0006] The non-ionic surfactant includes one or more of lauryl glucoside, PEG-6 caprylic / capric glyceride, PEG-7 glyceryl cocoate and polyglyceryl-10 esters of caprylic / capric glycerides; and / or

[0007] The amphoteric ion surfactant includes one or more of cocamidopropyl betaine and sodium lauroamphoacetate; and / or

[0008] The sophorolipid is prepared by fermenting glucose with C12 - C22 fatty acids or C12 - C22 oils and fats;

[0009] Optionally, the sophorolipid is prepared by fermenting glucose with C18 fatty acids or C18 oils and fats.

[0010] The second aspect of the present application provides an application of the washing and care composition of the first aspect of the present application in the preparation of washing and care products.

[0011] The third aspect of the present application provides a washing and care product, comprising a washing and care composition, the washing and care composition comprising sophorolipid, a first surfactant and a second surfactant, the first surfactant comprising an anionic surfactant, and the second surfactant comprising an amphoteric ion surfactant and / or a non-ionic surfactant; the mass ratio of the sophorolipid to the first surfactant is 1:(1-10), and the mass ratio of the sophorolipid to the second surfactant is 1:(1-10).

[0012] In some embodiments, the anionic surfactant comprises one or more of sodium cocoyl glutamate and potassium cocoyl glycinate; and / or

[0013] The non-ionic surfactant comprises one or more of lauryl glucoside, PEG-6 caprylic / capric glyceride, PEG-7 glyceryl cocoate and polyglyceryl-10 esters of caprylic / capric glycerides; and / or

[0014] The amphoteric ion surfactant comprises one or more of cocamidopropyl betaine and sodium lauroamphoacetate; and / or

[0015] The sophorolipid is prepared by fermenting glucose with C12-C22 fatty acids or C12-C22 oils and fats;

[0016] Optionally, the sophorolipid is prepared by fermenting glucose with C18 fatty acids or C18 oils and fats.

[0017] In some embodiments, the mass proportion of the sophorolipid in the washing and care product is 0.1%-5%, optionally 0.5%-2%; and / or

[0018] The mass proportion of the first surfactant in the washing and care product is 1%-5%; and / or

[0019] The mass proportion of the second surfactant in the washing and care product is 1%-5%.

[0020] In some embodiments, the washing and care product further comprises a humectant;

[0021] Optionally, the mass proportion of the humectant in the washing and care product is 0.5%-20%;

[0022] Optionally, the humectant comprises one or more of glycerol, trehalose and hyaluronic acid.

[0023] In some embodiments, the washing and care product further comprises a pH regulator;

[0024] Optionally, the pH regulator includes citric acid;

[0025] Optionally, the mass ratio of the pH regulator in the washing and care product is 0.1% - 0.5%.

[0026] In some embodiments, the washing and care product further contains a preservative;

[0027] Optionally, the preservative includes one or more of phenoxyethanol, dipropylene glycol, and iodopropynyl butylcarbamate;

[0028] Optionally, the mass ratio of the preservative in the washing and care product is 0.05% - 1.0%.

[0029] In some embodiments, the washing and care product further contains a chelating agent;

[0030] Optionally, the chelating agent includes one or more of disodium ethylenediaminetetraacetate and octanoyl hydroxamic acid;

[0031] Optionally, the mass ratio of the chelating agent in the washing and care product is 0.05% - 0.3%. In some embodiments, the washing and care product further contains water.

[0032] In some embodiments, the washing and care product includes one or more of cleansing water, shampoo, and body wash.

[0033] The fourth aspect of the present application provides an application of sophorolipid as an active ingredient for removing endotoxin.

[0034] The fifth aspect of the present application provides an application of sophorolipid in preparing a washing and care product for removing endotoxin.

[0035] For the above-mentioned washing and care composition, sophorolipid has the ability to directly and rapidly remove endotoxin. By compounding sophorolipid with a surfactant in a specific ratio, a synergistic effect can be exerted, significantly improving the endotoxin removal efficiency, thereby significantly reducing the occurrence of skin irritation and inflammatory reactions caused by endotoxin when the skin barrier is weak or damaged. Detailed Embodiments

[0036] To facilitate the understanding of the present invention, the present application will be described more comprehensively with reference to relevant embodiments below. The following gives the preferred embodiments of the present application. However, the present application can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the understanding of the disclosure content of the present application more thorough and comprehensive.

[0037] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the technical field to which this application belongs. The terms used in the specification of this application are for the purpose of describing specific embodiments only and are not intended to limit this application.

[0038] As used herein, the alternative of "and / or", "or / and", "and / or" includes any one of two or more related listed items, and also includes any and all combinations of the related listed items. The said any and all combinations include combinations of any two related listed items, any more related listed items, or all related listed items. It should be noted that when at least two conjunctions selected from "and / or", "or / and", "and / or" are used to connect at least three items, it should be understood that in this application, this technical solution undoubtedly includes the technical solution connected by "logical AND", and also undoubtedly includes the technical solution connected by "logical OR".

[0039] In this application, among the technical features described in an open-ended manner, it includes a closed technical solution composed of the listed features, and also includes an open technical solution containing the listed features.

[0040] In this application, regarding numerical ranges, unless otherwise specified, the above numerical ranges are considered continuous, and include the minimum and maximum values of the range, as well as each value between such minimum and maximum values. Further, when the range refers to integers, it includes each integer between the minimum and maximum values of the range. In addition, when multiple ranges are provided to describe features or characteristics, these ranges can be combined. In other words, unless otherwise specified, all ranges disclosed herein should be understood to include any and all sub-ranges subsumed therein.

[0041] This application specifically discloses only some numerical ranges. However, any lower limit can be combined with any upper limit to form a range not explicitly recorded; and any lower limit can be combined with other lower limits to form a range not explicitly recorded. Similarly, any upper limit can be combined with any other upper limit to form a range not explicitly recorded. In addition, each individually disclosed point or single numerical value itself can be used as a lower limit or an upper limit and combined with any other point or single numerical value or combined with other lower limits or upper limits to form a range not explicitly recorded.

[0042] The temperature parameter in this application, unless otherwise specified, allows both constant temperature treatment and treatment within a certain temperature range. The said constant temperature treatment allows the temperature to fluctuate within the accuracy range controlled by the instrument. Fluctuations within a range such as ±5°C, ±4°C, ±3°C, ±2°C, ±1°C are allowed.

[0043] In this application, the "suitable combination method", "suitable method", "any suitable method", etc., the "suitable" described therein shall be based on being able to implement the technical solution of this application, solve the technical problems of this application, and achieve the expected technical effects of this application.

[0044] In this application, "further", "even further", "especially", etc. are used for descriptive purposes, indicating differences in content, but should not be construed as limiting the scope of protection of this application.

[0045] In this application, "optionally", "optional", "option", mean that it can be either present or absent, that is, it refers to any one of the two parallel options of "present" or "absent". If "optional" appears multiple times in a technical solution, without special instructions and without contradictions or mutual constraints, each "optional" is independent of each other.

[0046] In the description of the application, the meaning of "a variety of" is at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0047] Unless otherwise specified, all preparations and tests in this article occur in an environment of 25 °C.

[0048] In this article, "include", "contain", "comprise", "have" or other variants are intended to cover non-closed inclusion, and there is no distinction between these terms. The term "contain" means that other steps and components can be added without affecting the final result. The compositions and methods / processes of the present invention contain, consist of, and consist essentially of the necessary elements and limitations described herein, as well as any additional or optional components, ingredients, steps, or limitations described herein. There is no distinction between the terms "efficacy", "performance", "effect", and "function" in this article.

[0049] If there is no special instruction, all implementation manners and optional implementation manners of this application can be combined with each other to form a new technical solution. If there is no special instruction, all technical features and optional technical features of this application can be combined with each other to form a new technical solution.

[0050] If there is no special instruction, all steps of this application can be carried out sequentially or randomly, and preferably sequentially.

[0051] When there are skin injuries, skin diseases, or long-term use of certain drugs that weaken the skin barrier, such as corticosteroids, etc., endotoxins from the external environment may penetrate through the damaged skin, causing irritation or allergic reactions, and even causing serious adverse reactions such as fever, microcirculation disorders, endotoxin shock, and disseminated intravascular coagulation.

[0052] Sophorolipid is a glycolipid biosurfactant produced by microbial fermentation, which has good cleaning performance, mildness and biocompatibility. In this application, sophorolipid is used to remove endotoxin and applied in the washing and care composition, greatly improving the ability of the composition to remove endotoxin, and thus effectively preventing skin irritation reactions caused by contact with endotoxin.

[0053] The first aspect of this application provides a washing and care composition, comprising sophorolipid, a first surfactant and a second surfactant. The first surfactant comprises an anionic surfactant, and the second surfactant comprises an amphoteric ion surfactant and / or a nonionic surfactant; the mass ratio of sophorolipid to the first surfactant is 1:(1 - 10), and the mass ratio of sophorolipid to the second surfactant is 1:(1 - 10).

[0054] As an example, the mass ratio of sophorolipid to the first surfactant can be, but is not limited to, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5, 1:10 or the range between any two of the above mass ratios, etc. Thus, when the mass ratio of sophorolipid to the first surfactant is within the above range, it can have a synergistic effect and improve the endotoxin removal effect.

[0055] The mass ratio of sophorolipid to the second surfactant can be, but is not limited to, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5, 1:10 or the range between any two of the above mass ratios, etc. When the mass ratio of sophorolipid to the second surfactant is within the above range, it can have a synergistic effect and improve the endotoxin removal effect.

[0056] It should be noted that in this article, in "the first surfactant", "the second surfactant", etc., the terms "first", "second", etc. are only for descriptive purposes and cannot be understood as indicating or implying relative importance or quantity, nor can they be understood as implicitly indicating the importance or quantity of the indicated technical features. Moreover, "first", "second", etc. only serve the purpose of non-exhaustive listing and description, and it should be understood that they do not constitute a closed limitation on quantity.

[0057] It can be understood that the sophorolipid in this washing and care composition has the ability to directly and rapidly remove endotoxin. By compounding sophorolipid with surfactants in a specific ratio, a synergistic effect can be exerted, significantly improving the endotoxin removal efficiency, and thus significantly reducing the occurrence of skin irritation and inflammatory reactions caused by endotoxin when the skin barrier is weak or damaged.

[0058] In some embodiments, the anionic surfactant includes one or more of sodium cocoyl glutamate and potassium cocoyl glycinate.

[0059] As a possible embodiment, the nonionic surfactant includes one or more of lauryl glucoside, PEG-6 caprylic / capric glycerides, PEG-7 glyceryl cocoate, and polyglyceryl-10 esters of caprylic / capric glycerides.

[0060] In some alternative embodiments, the zwitterionic surfactant includes one or more of cocamidopropyl betaine and sodium lauroamphoacetate.

[0061] In some exemplary embodiments, sophorolipid is prepared by fermenting glucose with C12-C22 fatty acids. That is, glucose and C12-C22 fatty acids are used as the fermentation substrates to prepare sophorolipid.

[0062] In some alternative embodiments, sophorolipid is prepared by fermenting glucose with C18 fatty acid.

[0063] In some alternative embodiments, sophorolipid is prepared by fermenting glucose with C12-C22 oils and fats. That is, glucose and C12-C22 oils and fats are used as the fermentation substrates to prepare sophorolipid.

[0064] In some of these embodiments, sophorolipid is prepared by fermenting glucose with C18 oils and fats.

[0065] One or more embodiments of the present application provide an application of the above-mentioned washing and care composition in the preparation of washing and care products.

[0066] Applying the above-mentioned washing and care composition to washing and care products can greatly improve the ability of the washing and care products to remove endotoxins, and thus have the effect of preventing skin irritation reactions caused by contact with endotoxins.

[0067] One or more embodiments of the present application provide a washing and care product, which includes a washing and care composition. The washing and care composition includes sophorolipid, a first surfactant, and a second surfactant. The first surfactant includes an anionic surfactant, and the second surfactant includes a zwitterionic surfactant and / or a nonionic surfactant; the mass ratio of sophorolipid to the first surfactant is 1:(1-10), and the mass ratio of sophorolipid to the second surfactant is 1:(1-10).

[0068] As an example, the mass ratio of sophorolipid to the first surfactant can be, but is not limited to, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5, 1:10, or the range between any two of the above mass ratios, etc. Thus, when the mass ratio of sophorolipid to the first surfactant is within the above range, it can achieve synergistic effects and improve the endotoxin removal effect.

[0069] The mass ratio of sophorolipid to the second surfactant can be, but is not limited to, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5, 1:10, or the range between any two of the above mass ratios, etc. Thus, when the mass ratio of sophorolipid to the second surfactant is within the above range, it can achieve synergistic effects and improve the endotoxin removal effect.

[0070] It can be understood that by introducing sophorolipid into the washing and care products, not only can part of the surfactant and humectant be replaced with sophorolipid, but also the efficacy of the washing and care products in removing endotoxin can be further improved, thereby avoiding skin irritation and inflammatory reactions caused by endotoxin.

[0071] In some embodiments, the anionic surfactant includes one or more of sodium cocoyl glutamate and potassium cocoyl glycinate.

[0072] As a possible embodiment, the non-ionic surfactant includes one or more of lauryl glucoside, PEG-6 caprylate / caprate glyceride, PEG-7 glyceryl cocoate, and polyglyceryl-10 esters of caprylic / capric glycerides.

[0073] In some alternative embodiments, the zwitterionic surfactant includes one or more of cocamidopropyl betaine and sodium lauroamphoacetate.

[0074] As a possible embodiment, sophorolipid is prepared by fermenting glucose with C12-C22 fatty acids.

[0075] In some alternative embodiments, sophorolipid is prepared by fermenting glucose with C18 fatty acid.

[0076] In some embodiments, sophorolipid is prepared by fermenting glucose with C12-C22 oils and fats.

[0077] In some of these embodiments, sophorolipid is prepared by fermenting glucose with C18 oils and fats.

[0078] In one or more embodiments of the present application, the mass ratio of sophorolipid in the washing and care product is 0.1% - 5%; for example, it can be, but is not limited to, 0.1%, 0.2%, 0.4%, 0.6%, 0.8%, 1%, 1.2%, 1.4%, 1.6%, 1.8%, 2%, 2.2%, 2.4%, 2.6%, 2.8%, 3%, 3.2%, 3.4%, 3.6%, 3.8%, 4%, 4.2%, 4.4%, 4.6%, 4.8%, 5% or the range between any two of the above mass ratios, etc. When the mass ratio of sophorolipid in the washing and care product is within the above range, it is beneficial to improve the ability of the washing and care product to remove endotoxins.

[0079] In some alternative embodiments, the mass ratio of sophorolipid in the washing and care product is 0.5% - 2%.

[0080] In some embodiments, the mass ratio of the first surfactant in the washing and care product is 1% - 5%; for example, it can be, but is not limited to, 1%, 1.2%, 1.4%, 1.6%, 1.8%, 2%, 2.2%, 2.4%, 2.6%, 2.8%, 3%, 3.2%, 3.4%, 3.6%, 3.8%, 4%, 4.2%, 4.4%, 4.6%, 4.8%, 5% or the range between any two of the above mass ratios, etc. When the mass ratio of the first surfactant in the washing and care product is within the above range, it is beneficial to improve the mildness, foaming ability and detergency of the washing and care product, and at the same time, it can be compounded with sophorolipid to synergistically improve the ability of the washing and care product to remove endotoxins.

[0081] In some embodiments, the mass ratio of the second surfactant in the washing and care product is 1% - 5%; for example, it can be, but is not limited to, 1%, 1.2%, 1.4%, 1.6%, 1.8%, 2%, 2.2%, 2.4%, 2.6%, 2.8%, 3%, 3.2%, 3.4%, 3.6%, 3.8%, 4%, 4.2%, 4.4%, 4.6%, 4.8%, 5% or the range between any two of the above mass ratios, etc. When the mass ratio of the second surfactant in the washing and care product is within the above range, it is beneficial to improve the detergency, emulsifying ability, dispersibility, mildness and antistatic ability of the washing and care product, and it can be compounded with sophorolipid to synergistically improve the ability of the washing and care product to remove endotoxins.

[0082] In some embodiments, the washing and care product further contains a humectant.

[0083] As a possible implementation, the mass ratio of the humectant in the washing and care product is 0.5% - 20%; for example, it can be, but is not limited to, 0.5%, 1%, 2%, 4%, 6%, 8%, 10%, 12%, 14%, 16%, 18%, 20% or the range between any two of the above mass ratios, etc.

[0084] In some alternative implementations, the humectant includes one or more of glycerol, trehalose, and hyaluronic acid.

[0085] In some implementations, the washing and care product further contains a pH regulator.

[0086] As a possible implementation, the pH regulator includes citric acid.

[0087] In some alternative implementations, the mass ratio of the pH regulator in the washing and care product is 0.1% - 0.5%; for example, it can be, but is not limited to, 0.1%, 0.2%, 0.3%, 0.4%, 0.5% or the range between any two of the above mass ratios, etc.

[0088] In some implementations, the washing and care product further contains a preservative.

[0089] As a possible implementation, the preservative includes one or more of phenoxyethanol, dipropylene glycol, and iodopropynyl butylcarbamate.

[0090] In some of these implementations, the mass ratio of the preservative in the washing and care product is 0.05% - 1.0%; for example, it can be, but is not limited to, 0.05%, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1.0% or the range between any two of the above mass ratios, etc.

[0091] In some implementations, the washing and care product further contains a chelating agent.

[0092] In some alternative implementations, the chelating agent includes one or more of disodium ethylenediaminetetraacetate and octanoyl hydroxamic acid.

[0093] In some of these implementations, the mass ratio of the chelating agent in the washing and care product is 0.05% - 0.3%. For example, it can be, but is not limited to, 0.05%, 0.1%, 0.15%, 0.2%, 0.25%, 0.3% or the range between any two of the above mass ratios, etc.

[0094] In some implementations, the washing and care product includes one or more of cleansing water, shampoo, and body wash.

[0095] One or more embodiments of the present application provide an application of sophorolipid as an active ingredient for endotoxin clearance.

[0096] One or more embodiments of the present application provide an application of sophorolipid in the preparation of a washing and care product for endotoxin clearance.

[0097] The technical solutions of the present invention are described in detail below in conjunction with specific embodiments. It should be understood that these embodiments are only used to illustrate the present invention and not to limit the scope of the present invention. For the experimental methods without specific conditions in the following embodiments, the guidance given in the present invention is preferably referred to, and it can also be carried out according to the experimental manuals or conventional conditions in the art, or according to the conditions recommended by the manufacturers, or referring to the experimental methods known in the art.

[0098] In the following specific embodiments, for the measurement parameters of the raw material components, if there is no special explanation, there may be slight deviations within the weighing accuracy range. For the temperature and time parameters, acceptable deviations caused by instrument test accuracy or operation accuracy are allowed.

[0099] It should be noted that the raw materials used in the following test examples, embodiments and comparative examples can all be purchased from the market.

[0100] It should be noted that in the following test examples, embodiments and comparative examples, SL represents sophorolipid, LG represents lauryl glucoside; DCG represents disodium cocoyl glutamate; CB represents cocamidopropyl betaine; PEG-6 C / CG represents PEG-6 caprylic / capric glyceride; CCG-10E represents polyglycerol-10 esters of caprylic / capric glycerides; PEG-7 GC represents PEG-7 glyceryl cocoate.

[0101] I. Test Examples

[0102] Test Example 1

[0103] Sophorolipid, lauryl glucoside, PEG-6 caprylic / capric glyceride, PEG-7 glyceryl cocoate, polyglycerol-10 esters of caprylic / capric glycerides, cocamidopropyl betaine and disodium cocoyl glutamate were respectively mixed with water to prepare an endotoxin detection reagent with a mass percentage concentration of 0.5% for endotoxin clearance ability detection. The detection method is as follows:

[0104] 1. Sample preparation: Adjust the pH range of the sample to be between 6 and 8, prepare a 1 EU endotoxin solution using an endotoxin standard product, and add it to the sample in a 1:1 ratio (i.e., the sample contains 0.5 EU endotoxin), and mix well.

[0105] 2. The limulus reagent colorimetric method is used to detect the endotoxin content. The detection method is as follows:

[0106] Standard preparation: Prepare endotoxin standard solutions with concentrations of 1.0, 0.5, 0.25, and 0.10 EU / mL from the 20 EU / mL endotoxin standard solution. Sample: Shake for 2 - 3 min to fully dissolve. Take 10 μL and add it to 190 μL of endotoxin test water to prepare a 1 EU / mL stock solution.

[0107] Detection steps: a) Take an endotoxin - free centrifuge tube or PCR tube, add 10 μL of endotoxin test water, endotoxin standard solution, or sample to be tested. Then add 10 μL of endotoxin detection reagent solution, mix well, cover with aluminum foil, and incubate at 37 °C in the dark for 9 min. After incubation, add 10 μL of chromogenic reagent solution, mix well, and incubate at 37 °C for 6 min. After incubation, add 50 μL of Buffer A solution and mix well. b) Add 50 μL of Buffer B solution and mix well. c) Add 50 μL of Buffer C solution, mix well, let stand for 5 minutes, and read the absorbance value at a wavelength of 545 nm using a spectrophotometer or microplate reader. The time from the end of the color reaction to reading the absorbance value should not exceed 5 h.

[0108] 3. The test results are shown in Table 1.

[0109] Test Example 2

[0110] Respectively mix sophorolipid, lauryl glucoside, PEG - 6 caprylic / capric glyceride, PEG - 7 glyceryl cocoate, caprylic / capric glyceride polyglycerol - 10 esters, cocamidopropyl betaine, and disodium cocoyl glutamate with water to prepare endotoxin detection reagents with a mass percentage concentration of 1.0% for endotoxin clearance ability detection. The detection method is the same as that in Test Example 1. The test results are shown in Table 1.

[0111] Test Example 3

[0112] Respectively mix sophorolipid, lauryl glucoside, PEG - 6 caprylic / capric glyceride, PEG - 7 glyceryl cocoate, caprylic / capric glyceride polyglycerol - 10 esters, cocamidopropyl betaine, and disodium cocoyl glutamate with water to prepare endotoxin detection reagent solutions with a mass percentage concentration of 2.0% for endotoxin clearance ability detection. The detection method is the same as that in Test Example 1. The test results are shown in Table 1.

[0113] Table 1

[0114]

[0115] As can be seen from the results in Table 1, when the mass percentage concentration is 0.5%, for the ability to remove endotoxin within 0.5 EU, sophorolipid (44.72%) > cocamidopropyl betaine (43.99%) > lauryl glucoside (19.03%) > disodium cocoyl glutamate (17.23%) > PEG-7 glyceryl cocoate (13.49%) > caprylic / capric glycerides polyglyceryl-10 esters (12.75%) > PEG-6 caprylic / capric glyceride (4.45%).

[0116] When the mass percentage concentration is 1.0%, for the ability to remove endotoxin within 0.5 EU, sophorolipid (83.04%) > lauryl glucoside (38.99%) > PEG-7 glyceryl cocoate (37.45%) > cocamidopropyl betaine (33.32%) > caprylic / capric glycerides polyglyceryl-10 esters (31.73%) > disodium cocoyl glutamate (29.85%) > PEG-6 caprylic / capric glyceride (17.11%).

[0117] When the mass percentage concentration is 2.0%, for the ability to remove endotoxin within 0.5 EU, sophorolipid (124.07%) > cocamidopropyl betaine (62.46%) > lauryl glucoside (61.76%) > disodium cocoyl glutamate (41.96%) > caprylic / capric glycerides polyglyceryl-10 esters (38.72%) > PEG-7 glyceryl cocoate (36.84%) > PEG-6 caprylic / capric glyceride (8.03%).

[0118] Six endotoxin detection reagent solutions with a mass percentage concentration of 0.5% of sophorolipid were prepared, and then lauryl glucoside, PEG-6 caprylic / capric glyceride, PEG-7 glyceryl cocoate, caprylic / capric glycerides polyglyceryl-10 esters, cocamidopropyl betaine, and disodium cocoyl glutamate were added respectively to obtain six mixtures. The mass percentage concentration of lauryl glucoside, PEG-6 caprylic / capric glyceride, PEG-7 glyceryl cocoate, caprylic / capric glycerides polyglyceryl-10 esters, cocamidopropyl betaine, or disodium cocoyl glutamate in each mixture was 0.5%. The endotoxin removal ability of each mixture was detected according to the detection method in Test Example 1, and the detection results are shown in Table 2.

[0119] Table 2

[0120]

[0121] It can be seen from the result comparison between Table 1 and Table 2 that the endotoxin removal effects of the above compound combinations are all better than the sum of the endotoxin removal effects of each component in the compound combination when used alone. It shows that after the sophorolipid is compounded with an anionic surfactant, an amphoteric ion surfactant or a non-ionic surfactant, the endotoxin removal ability is significantly improved, achieving a synergistic effect.

[0122] II. Preparation of the washing and care products

[0123] 1. Formulation composition

[0124] The formulation compositions of each example and comparative example are shown in Table 3.

[0125] Table 3

[0126]

[0127] Table 4

[0128]

[0129] 2. Preparation process

[0130] Prepare according to the following steps: S1 Heat the first surfactant and the second surfactant to 55°C, then add the dissolved surfactant and sophorolipid into the beaker, and stir while heating; S2 Add 50 wt% of the water in the formula amount to the beaker under stirring; S3 Add the humectant, pH regulator, preservative and chelating agent to the beaker under stirring, and make up the water to the formula amount; S4 Cool to room temperature while stirring until all raw materials are evenly dispersed; S5 Transfer to the sample bottle after standing and defoaming.

[0131] 3. Using the endotoxin removal ability detection method recorded in Test Example 1, the endotoxin removal effects of the washing and care products prepared in the above examples and comparative examples were tested, and the results are shown in Table 5.

[0132] Table 5

[0133]

[0134] It can be seen from the result comparison between Example 1 and Comparative Example 1, Example 2 and Comparative Example 2, Example 3 and Comparative Example 3, and Example 4 and Comparative Example 4 in Table 5 that compared with only using a surfactant without using sophorolipid, the endotoxin removal rate is significantly improved when using sophorolipid and surfactant at the same time.

[0135] It can be seen from the result comparison between Examples 1-7 and Comparative Example 5 that compared with only using sophorolipid, the endotoxin removal rate is significantly improved when using sophorolipid and surfactant at the same time.

[0136] From the comparison of the results of Examples 1-10 and Comparative Examples 6-7, it can be seen that when the mass ratio of sophorolipid to the first surfactant needs to be regulated to 1:(1-10) and the mass ratio of sophorolipid to the second surfactant is 1:(1-10), the combination of sophorolipid and the surfactant can play a synergistic effect. Therefore, the above advantages indicate that the combination of sophorolipid and the surfactant in a specific ratio can play a synergistic effect and significantly improve the endotoxin clearance efficiency.

[0137] The technical features of the above-described embodiments can be combined arbitrarily. For the sake of concise description, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, it should be considered as the scope described in this specification.

[0138] The above-described embodiments only represent several implementation manners of the present invention. The description is relatively specific and detailed, but it cannot be understood as a limitation on the scope of the invention patent. It should be noted that for those of ordinary skill in the art, without departing from the concept of the present invention, several modifications and improvements can still be made, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the present invention patent shall be subject to the appended claims.

Claims

1. A cleaning composition, characterized in that: It includes a sophorolipid, a first surfactant and a second surfactant, wherein the first surfactant includes an anionic surfactant, and the second surfactant includes a zwitterionic surfactant and / or a nonionic surfactant; the mass ratio of the sophorolipid to the first surfactant is 1:(1-10), and the mass ratio of the sophorolipid to the second surfactant is 1:(1-10).

2. The cleaning composition according to claim 1, characterized in that The anionic surfactant comprises one or more of disodium cocoyl glutamate and potassium cocoyl glycinate; and / or The nonionic surfactant comprises one or more of lauryl glucoside, PEG-6 caprylic / capric glycerides, PEG-7 glyceryl cocoate and caprylic / capric glycerides polyglyceryl-10 esters; and / or The zwitterionic surfactant comprises one or more of cocamidopropyl betaine and sodium lauroamphoacetate; and / or The sophorolipid is prepared by fermenting glucose with C12-C22 fatty acids or C12-C22 oils; Optionally, the sophorolipid is produced by fermenting glucose with C18 fatty acid or C18 oil.

3. Use of the cleaning composition according to any one of claims 1 to 2 in the preparation of cleaning products.

4. A cleaning product, characterized in that: The invention comprises a washing and care composition, wherein the washing and care composition comprises a sophorolipid, a first surfactant and a second surfactant, wherein the first surfactant comprises an anionic surfactant, and the second surfactant comprises a zwitterionic surfactant and / or a nonionic surfactant; the mass ratio of the sophorolipid to the first surfactant is 1:(1-10), and the mass ratio of the sophorolipid to the second surfactant is 1:(1-10).

5. The cleaning product according to claim 4, characterized in that: The anionic surfactant comprises one or more of disodium cocoyl glutamate and potassium cocoyl glycinate; and / or The nonionic surfactant comprises one or more of lauryl glucoside, PEG-6 caprylic / capric glycerides, PEG-7 glyceryl cocoate and caprylic / capric glycerides polyglyceryl-10 esters; and / or The zwitterionic surfactant includes one or more of cocamidopropyl betaine and sodium lauroamphoacetate; and / or The sophorolipid is prepared by fermenting glucose with C12-C22 fatty acids or C12-C22 oils; Optionally, the sophorolipid is produced by fermenting glucose with C18 fatty acid or C18 oil.

6. The cleaning product according to claim 4, characterized in that: The mass proportion of the sophorolipid in the cleaning product is 0.1%-5%, and can be 0.5%-2%; and / or The mass proportion of the first surfactant in the cleaning product is 1%-5%; and / or The mass proportion of the second surfactant in the cleaning product is 1%-5%.

7. The cleaning product according to claim 4, characterized in that: The cleaning product further comprises a moisturizer; Optionally, the mass proportion of the moisturizer in the cleaning product is 0.5%-20%; Optionally, the moisturizing agent includes one or more of glycerin, trehalose and hyaluronic acid.

8. The cleaning product according to claim 4, characterized in that: The cleaning product further comprises a pH adjuster; Optionally, the pH adjuster includes citric acid; Optionally, the pH adjuster accounts for 0.1%-0.5% by mass in the cleaning product.

9. The cleaning product according to claim 4, characterized in that: The cleaning product further comprises a preservative; Optionally, the preservative includes one or more of phenoxyethanol, dipropylene glycol and iodopropynyl butylcarbamate; Optionally, the mass proportion of the preservative in the cleaning product is 0.05%-1.0%.

10. The cleaning product according to claim 4, characterized in that: The cleaning product further comprises a chelating agent; Optionally, the chelating agent includes one or more of disodium ethylenediaminetetraacetic acid and caprylhydroxamic acid; Optionally, the mass proportion of the chelating agent in the cleaning product is 0.05%-0.3%.

11. The cleaning product according to any one of claims 4 to 10, characterized in that: The cleaning products include one or more of facial cleansing water, shampoo and shower gel.

12. Use of a sophorolipid as an effective ingredient for removing endotoxin.

13. Use of a sophorolipid in the preparation of a cleaning product for removing endotoxins.

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