Anti-wrinkle composition as well as preparation method, application and cosmetic thereof

Abstract

The invention belongs to the technical field of cosmetic production, and discloses an anti-wrinkle composition and a preparation method and application thereof as well as cosmetics, the anti-wrinkle composition comprises a flos magnoliae extract and an emblic leafflower fruit extract, and the mass ratio of the flos magnoliae extract to the emblic leafflower fruit extract is (0.1-8): (0.5-10); through the synergistic effect of flos magnoliae and fructus phyllanthi contained in the composition, the composition not only has good elastinase inhibition ability, but also has excellent antioxidant ability, so that the composition has good elastinase inhibition ability and good free radical scavenging ability, and further achieves good anti-wrinkle performance.

Description

Technical Field

[0001] This application relates to the technical field of cosmetic production, and particularly relates to an anti-wrinkle composition, a preparation method, an application thereof, and a cosmetic. Background Art

[0002] Elastase is a protease released by inflammatory cells such as neutrophils and macrophages, and its main function is to decompose elastin in the extracellular matrix. Elastin is an important structural protein in the dermis of the skin, endowing the skin with elasticity, firmness, and resilience. With age, the skin's self-repair ability declines, the synthesis of elastin decreases, while its decomposition increases, leading to skin laxity and wrinkle formation. By inhibiting the activity of elastase, reducing its degradation of elastin, maintaining skin elasticity and firmness, and the retention of elastin can reduce fine lines and deep wrinkles caused by structural collapse.

[0003] Free radicals (such as reactive oxygen species ROS and reactive nitrogen species RNS) are strongly oxidizing molecules produced by metabolic products or external stimuli. They trigger oxidative stress reactions by attacking lipids, proteins, and DNA within cells. Free radicals can directly damage collagen fibers and elastic fibers, causing the skin to lose its supporting force. At the same time, mitochondrial DNA damage and cell membrane lipid peroxidation will accelerate cell apoptosis or aging. Neutralizing free radicals can protect collagen, elastin, and cell membrane structures, delaying skin laxity and wrinkles.

[0004] Prior Art 1: Chinese Patent Application No. 202010850281.5 discloses a plant-derived anti-aging composition and its application. The composition contains NMN and plant extracts, and the plant extracts are any one or any combination of rosemary extract, cyanotis arachnoidea extract, anoectochilus roxburghii extract, and phyllanthus emblica extract;

[0005] Further observing the description of this scheme in the specification, it is recorded that the phyllanthus emblica extract can significantly increase the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-px) in serum and tissues, and significantly reduce the contents of malondialdehyde (MAD) and lipoxin (LPF). The phyllanthus emblica extract contains a large amount of polyphenolic substances and has significant antioxidant, anti-inflammatory, antibacterial, antiviral, and anti-aging effects.

[0006] Prior Art 2: Chinese Patent Application No. 202410362678.8 discloses a method for extracting magnolia biondii, an extract thereof, and products. Further observing the description part of this scheme, as it is found through testing that the magnolia biondii extract has a certain soothing ability, and it is also found that the magnolia biondii extract has a certain firming ability, and it is further verified that the magnolia biondii extract has a certain anti-wrinkle effect.

[0007] In summary, the prior art discloses that Phyllanthus emblica and Magnolia biondii each have certain anti-aging abilities. The anti-aging mechanism of Phyllanthus emblica mainly stems from its good antioxidant mechanism;

[0008] while Magnolia biondii shows certain firming and anti-wrinkle abilities, but the prior art does not disclose the antioxidant performance of Magnolia biondii;

[0009] More importantly, the prior art has not tried whether the mixture of Magnolia biondii flowers and Phyllanthus emblica fruits still has good anti-wrinkle ability.

[0010] The problem to be solved by this solution: How to provide an anti-wrinkle composition with good firming ability. Summary of the Invention

[0011] The purpose of this application is to provide an anti-wrinkle composition. Through the synergistic effect between the Magnolia biondii flowers and Phyllanthus emblica fruits contained in this composition, while having good elastase inhibitory ability, it also has excellent antioxidant ability, so that the composition has good elastase inhibitory ability and good free radical scavenging ability. Since free radicals can damage collagen and elastic fibers, leading to skin relaxation and accelerating wrinkle formation; antioxidant components can neutralize free radicals and reduce their damage to the skin structure. Therefore, the anti-wrinkle composition provided by this application is expected to achieve anti-wrinkle effects from two aspects: inhibiting elastase and scavenging free radicals.

[0012] To achieve the above purpose, this application discloses an anti-wrinkle composition, including Magnolia biondii flower extract and Phyllanthus emblica fruit extract, and the mass ratio of the Magnolia biondii flower extract to the Phyllanthus emblica fruit extract is 0.1-8:0.5-10.

[0013] When the Magnolia biondii flower extract and the Phyllanthus emblica fruit extract are used together, on the one hand, due to their good free radical scavenging ability, oxidative stress is reduced from the source, and the release of elastase is inhibited; and because both have good elastase inhibitory ability, the destruction of elastin is blocked at the terminal, forming a double defense line to further enhance the anti-wrinkle ability of the composition.

[0014] Preferably, it also includes Syringa oblata Lindl. flower extract, and the mass ratio of the Magnolia biondii flower extract, the Phyllanthus emblica fruit extract, and the Syringa oblata Lindl. flower extract is 0.1-8:0.5-10:0.5-14.

[0015] Syringa oblata Lindl. flower extract is a mixture of multiple components, and some of its components can interact with elastase. Some of the terpenoid compounds it contains can inhibit the hydrolysis of elastin by elastase.

[0016] In addition, the present application also discloses a preparation method for preparing the above-mentioned anti-wrinkle composition, which comprises mixing and dispersing the extract of Magnolia biondii flowers and the extract of Phyllanthus emblica fruits to obtain the anti-wrinkle composition.

[0017] Preferably, the extraction method of the extract of Magnolia biondii flowers is as follows: drying the Magnolia biondii flowers, then placing them in an alcohol solvent, heating, stirring and centrifuging to obtain an intermediate, and then immersing the intermediate in a fat-soluble solvent, stirring, extracting and centrifuging to obtain the extract of Magnolia biondii flowers;

[0018] The extraction method of the extract of Phyllanthus emblica fruits is as follows: drying the Phyllanthus emblica fruits, then placing them in an alcohol solvent, heating, stirring and centrifuging to obtain an intermediate, and then immersing the intermediate in a fat-soluble solvent, stirring, extracting and centrifuging to obtain the extract of Phyllanthus emblica fruits;

[0019] The extraction method of the extract of Syringa oblata flowers is as follows: drying the Syringa oblata flowers, then placing them in an alcohol solvent, heating, stirring and centrifuging to obtain an intermediate, and then immersing the intermediate in a fat-soluble solvent, stirring, extracting and centrifuging to obtain the extract of Syringa oblata flowers.

[0020] Preferably, the fat-soluble solvent is selected from at least one of triglyceride (ethylhexanoate), caprylic / capric triglyceride, octyldodecanol, coco-caprylate / caprate, ethylhexyl triazone, cetyl ethylhexanoate, and Limnanthes alba seed oil.

[0021] In addition, the present application also discloses the use of the above-mentioned anti-wrinkle composition in preparing cosmetics.

[0022] In addition, the present application also discloses a cosmetic containing 0.1-20 wt% of the above-mentioned anti-wrinkle composition.

[0023] Preferably, the dosage form of the cosmetic is a patch, ointment, liquid, spray, gel, emulsion or cream.

[0024] Preferably, the cosmetic is a gel, essence oil, facial mask, facial cleanser, lipstick or blush.

[0025] The beneficial effects of the present application are as follows:

[0026] When the extract of Magnolia biondii flowers and the extract of Phyllanthus emblica fruits are used together, on the one hand, due to their good free radical scavenging ability, oxidative stress is reduced from the source, and the release of elastase is inhibited; and due to their good elastase inhibitory ability, the destruction of elastin is blocked at the terminal, forming a double defense line, further enhancing the anti-wrinkle ability of the composition. Description of the Drawings

[0027] Figure 1 It is a pre-detection chicken embryo diagram of the sample corresponding to Example 5 during the irritation test;

[0028] Figure 2 It is the chicken embryo diagram after the detection of the corresponding sample in Example 5 during the irritation test. Detailed implementation manners

[0029] Next, the embodiments of the present application will be described clearly and completely in conjunction with the embodiments of the present application. In the description of the present application, it should be noted that for those conditions not specified in the embodiments, they are carried out according to conventional conditions or the conditions recommended by the manufacturer. For the reagents or instruments whose manufacturers are not specified, they are all conventional products that can be obtained through commercial purchase.

[0030] Before presenting the embodiments, the following necessary explanations will be made on the preparation and acquisition routes of the raw materials involved in the embodiments:

[0031] Magnolia biondii flower: Bozhou Yuanshengtang Pharmaceutical Co., Ltd.;

[0032] Phyllanthus emblica fruit: Bozhou Yuanshengtang Pharmaceutical Co., Ltd.;

[0033] Syringa oblata flower: Bozhou Yuanshengtang Pharmaceutical Co., Ltd.;

[0034] Ethanol: Guangxi Jinyuan Biochemical Industry Co., Ltd., with a purity of more than 95%;

[0035] Tris(ethylhexanoate) glycerol (TIO): Guangzhou Hemiao Biotechnology Co., Ltd.;

[0036] Preparation method of the anti-wrinkle composition (except as otherwise specified, the compositions in the following examples and comparative examples are all prepared by this method):

[0037] Extraction method of Magnolia biondii flower extract:

[0038] Dry and crush the Magnolia biondii flower, then immerse the Magnolia biondii flower in an ethanol solution with a mass fraction of 95% and stir at 40°C for 3 h, then centrifuge, and heat at 65°C to accelerate the evaporation of the ethanol solution to obtain an intermediate. It should be noted that the addition amount of the ethanol solution should be such that it can completely immerse the crushed Magnolia biondii flower. In the actual operation process of this case, the mass ratio between the crushed Magnolia biondii flower and the ethanol solution is 1:25;

[0039] Subsequently, immerse the intermediate in the TIO solvent, stir and extract for 1 h, then centrifuge to remove insoluble substances to obtain the Magnolia biondii flower extract. Similarly, it should be noted that the addition amount of TIO should be such that it can completely immerse the intermediate. In the actual operation process of this case, the mass ratio between the intermediate and TIO is 1:35.

[0040] Extraction method of Phyllanthus emblica fruit extract:

[0041] Dry and crush Phyllanthus emblica fruits, then immerse the Phyllanthus emblica fruits in an ethanol solution with a mass fraction of 95% and stir at 40 °C for 3 h. Then centrifuge and heat at 55 °C to volatilize the ethanol solution to obtain an intermediate. It should be noted that the addition amount of the ethanol solution should be such that it can completely immerse the crushed Phyllanthus emblica fruits. In the actual operation process of this case, the mass ratio between the crushed Phyllanthus emblica fruits and the ethanol solution is 1:30;

[0042] Then immerse the intermediate in a TIO solvent, stir and extract for 1 h, then centrifuge to remove insoluble substances to obtain a Phyllanthus emblica fruit extract. Similarly, it should be noted that the addition amount of TIO should be such that it can completely immerse the intermediate. In the actual operation process of this case, the mass ratio between the intermediate and TIO is 1:30.

[0043] Extraction method of Syringa vulgaris extract:

[0044] Dry and crush Syringa vulgaris flowers, then immerse the Syringa vulgaris flowers in an ethanol solution with a mass fraction of 95% and stir at 40 °C for 3 h. Then centrifuge and heat at 60 °C to volatilize the ethanol solution to obtain an intermediate. It should be noted that the addition amount of the ethanol solution should be such that it can completely immerse the crushed Syringa vulgaris flowers. In the actual operation process of this case, the mass ratio between the crushed Syringa vulgaris flowers and the ethanol solution is 1:20;

[0045] Then immerse the intermediate in a TIO solvent, stir and extract for 1 h, then centrifuge to remove insoluble substances to obtain a Syringa vulgaris flower extract. Similarly, it should be noted that the addition amount of TIO should be such that it can completely immerse the intermediate. In the actual operation process of this case, the mass ratio between the intermediate and TIO is 1:25;

[0046] The preparation method of the anti-wrinkle composition is as follows: Mix and disperse the Magnolia biondii flower extract and the Phyllanthus emblica fruit extract to obtain the anti-wrinkle composition.

[0047] Examples 1 - 6

[0048] An anti-wrinkle composition, the raw materials and the mass ratios between the raw materials are shown in Table 1:

[0049] Table 1: Formulation table of the compositions in Examples 1 - 6

[0050]

[0051] Comparative Examples 1 - 5

[0052] A composition, the raw materials and the mass ratios between the raw materials are shown in Table 2:

[0053] Table 2: Formulation table of the compositions in Comparative Examples 1 - 5

[0054]

[0055]

[0056] Performance test:

[0057] 1. Irritation test

[0058] 1.1 Instruments and equipment

[0059] Fully automatic incubator, stereomicroscope, SPF chicken embryos;

[0060] 1.2 Reagents

[0061] Sodium chloride, sodium dodecyl sulfate (SDS);

[0062] 1.3 Incubation conditions

[0063] Room temperature: 20°C - 25°C, relative humidity: 45% - 70%, incubation temperature: 37.5°C ± 0.5°C, relative humidity: 55% - 70%, turntable rotation rate: 3 times / h - 6 times / h. Chicken embryos at 9 days old do not need to be rotated during incubation.

[0064] 1.4 Test methods

[0065] (1) Test operation steps

[0066] For each group of this test, 6 embryos are selected. The situation of the chorioallantoic membrane is recorded with a photographing device. A polytetrafluoroethylene resin ring is placed on the chorioallantoic membrane of the chicken embryo and photographed. The anti-wrinkle composition prepared in Example 5 is diluted with peanut oil to an oil solution with a mass fraction of 2% of the composition and used as the test sample. Subsequently, the test sample is added into the polytetrafluoroethylene resin ring, the time of adding the sample is recorded, and the air chamber is covered with a wet plastic wrap. The chicken embryo is transferred to an incubator with constant temperature and humidity for cultivation, and the degree of change in each toxic effect is observed.

[0067] (2) Result observation

[0068] Observe and record the manifestations of bleeding, blood coagulation and vascular lysis, and score according to their severity.

[0069] The scoring criteria for bleeding, blood coagulation and vascular lysis are shown in Table 3:

[0070] Table 3: Scoring criteria for bleeding, blood coagulation and vascular lysis

[0071]

[0072] Scoring criteria: ES ≤ 12: No / mild irritation; 12 < ES < 16: Moderate irritation; ES ≥ 16: Strong irritation.

[0073] Reference Figure 1-2, after detection, the ES value of the sample corresponding to Example 5 was 2.00, which was less than 12, indicating no irritation.

[0074] 2. Elastase Inhibition Rate Test

[0075] 2.1 Instrumentation

[0076] BSA224S Analytical Balance, RT-6100 Microplate Reader;

[0077] 2.2 Reagents

[0078] Elastase (porcine pancreas), BR, N-Succinyl-L-alanyl-L-alanyl-L-alanine, 98%;

[0079] 2.3 Test Method

[0080] (1) Treatment of Control and Test Samples

[0081] Sample Group: Dilute the sample (the composition prepared in the examples and comparative examples) with ethanol to a sample mass fraction of 5%;

[0082] Negative Control: Ethanol.

[0083] (2) Test Operation Steps

[0084] Set up a sample group, a sample background group, a solvent group, and a solvent background group. Each group needs to have 3 parallels. Add different reagent solutions to a 96-well plate, shake gently, incubate at 25 °C for 15 min, then place it in a microplate reader and measure the absorbance at 410 nm.

[0085] (3) The calculation formula is shown in Equation 1:

[0086]

[0087] In the formula: A—the absorbance of the reaction solution without the sample and containing the enzyme;

[0088] B—the absorbance of the reaction solution without the sample and the enzyme;

[0089] C—the absorbance of the reaction solution containing the sample and the enzyme;

[0090] D—the absorbance of the reaction solution containing the sample and without the enzyme.

[0091] The test results are shown in Table 4:

[0092] Table 4: Elastase Inhibition Rate Test Results Table

[0093]

[0094]

[0095] Result analysis:

[0096] 1. As can be seen from Examples 1 - 3, when the mass ratio between the magnolia flower extract and the phyllanthus emblica fruit extract is slightly adjusted, the elastase inhibition rates of Examples 1 - 3 show a certain degree of fluctuating trend, and when the mass ratio between the magnolia flower extract and the phyllanthus emblica fruit extract in Example 2 is 4:4, the composition undoubtedly has a more excellent elastase inhibition ability;

[0097] Further observing Examples 4 - 6, on the basis of Example 2, Examples 4 - 6 further introduce the lilac flower extract, and when the mass ratio between the magnolia flower extract, the phyllanthus emblica fruit extract, and the lilac flower extract is 4:4:4, the elastase inhibition ability of the composition is most significantly improved;

[0098] 2. As can be seen from Example 2 and Comparative Examples 1 - 2, when the compositions of Comparative Examples 1 - 2 only contain the magnolia flower extract or the phyllanthus emblica fruit extract, the elastase inhibition rates of Comparative Examples 1 - 2 are only 23.57% and 27.97% respectively;

[0099] When the composition of Example 2 is a mixture with a mass ratio of 4:4 between the magnolia flower extract and the phyllanthus emblica fruit extract, the elastase inhibition rate of Example 2 reaches 35.14%, which is much higher than any one of Comparative Examples 1 and 2;

[0100] It can be seen that there is a synergistic effect between the magnolia flower extract and the phyllanthus emblica fruit extract, which significantly improves the elastase inhibition ability of the composition prepared in Example 2;

[0101] 3. As can be seen from Example 5, Comparative Examples 1 - 2, and Comparative Example 5, when the compositions of Comparative Examples 1 - 2 only contain the magnolia flower extract or the phyllanthus emblica fruit extract, the elastase inhibition rates of Comparative Examples 1 - 2 are only 23.57% and 27.97% respectively;

[0102] When the composition of Comparative Example 5 only contains the lilac flower extract, the elastase inhibition rate of Comparative Example 5 is 25.24%; when Example 5 is composed of the magnolia flower extract, the phyllanthus emblica fruit extract, and the lilac flower extract at the same time, its elastase inhibition rate reaches 41.29%, and according to the elastase inhibition ability of the compositions of Comparative Examples 1 - 2 and Comparative Example 5, the theoretical value of the elastase inhibition rate of Example 5 should be (23.57% + 27.97% + 25.24%) / 3 = 25.59%;

[0103] It can be seen that the elastase inhibition rate of Example 5 exceeds its theoretical value, that is, the elastase inhibition ability of the composition prepared in Example 5 exceeds the simple superposition of Comparative Examples 1-2 and Comparative Example 5;

[0104] Thus, there is a synergistic effect among the extracts of Magnolia biondii Pamp., Phyllanthus emblica L. fruit, and Syringa vulgaris L. flower;

[0105] Further observing Comparative Examples 3-4, it can be seen that when the extract of Magnolia biondii Pamp. or the extract of Phyllanthus emblica L. fruit is used in combination with the extract of Syringa vulgaris L. flower, the elastase inhibition rate of Comparative Example 3 is 24.20%, and its inhibitory ability against elastase is between that of Comparative Example 1 and Comparative Example 5. It can be seen that there is no obvious synergistic effect between the extract of Magnolia biondii Pamp. and the extract of Syringa vulgaris L. flower in Comparative Example 3;

[0106] The elastase inhibition rate of Comparative Example 4 reaches 30.02%, which is significantly higher than any one of Comparative Example 2 and Comparative Example 5. It can be seen that there is a relatively strong synergistic effect between the extract of Phyllanthus emblica L. fruit and the extract of Syringa vulgaris L. flower in Comparative Example 4;

[0107] In summary, there is a synergistic effect between the extract of Magnolia biondii Pamp. and the extract of Phyllanthus emblica L. fruit;

[0108] 1. There is a synergistic effect between the extract of Phyllanthus emblica L. fruit and the extract of Syringa vulgaris L. flower, but the synergistic effect is relatively poor compared with that between the extract of Phyllanthus emblica L. fruit and the extract of Magnolia biondii Pamp.;

[0109] 2. It is difficult to show a strong synergistic effect when the extract of Syringa vulgaris L. flower and the extract of Magnolia biondii Pamp. act together. However, when the extract of Phyllanthus emblica L. fruit is added to the composition, the synergistic effect of the three can be more fully exerted.

[0110] 3. DPPH free radical scavenging rate test

[0111] 3.1 Instrument and equipment

[0112] BSA224S analytical balance, L6s ultraviolet spectrophotometer

[0113] 3.2 Reagents

[0114] DPPH (1,1-diphenyl-2-picrylhydrazine), 98%

[0115] 3.3 Test method

[0116] (1) Treatment of control and test samples

[0117] Sample group: Dilute the sample (the composition prepared in the examples and comparative examples) with ethanol to a sample mass fraction of 5%;

[0118] Negative control: Ethanol.

[0119] (2) Experimental operation steps

[0120] Set up a sample group and a negative control group. Three parallel tubes should be set up for each group. Add the corresponding reagent solutions to each group respectively, shake gently, and let stand at room temperature for 5 minutes. Transfer the reaction solutions of each group into 1 cm cuvettes and measure the absorbance at 517 nm.

[0121] (3) The calculation formula is shown in Equation 2:

[0122]

[0123] Where: T—the absorbance of the sample tube, that is, the absorbance of the solution after the sample reacts with DPPH;

[0124] T0—the background absorbance of the sample;

[0125] C—the average value of the absorbance of the DPPH tube three times, that is, the absorbance of the DPPH solution without adding the sample;

[0126] C0—the background absorbance of the solvent.

[0127] The test results are shown in Table 5:

[0128] Table 5: Test result table of DPPH free radical scavenging rate

[0129]

[0130]

[0131] Result analysis:

[0132] 1. It can be seen from Examples 1-3 that when the mass ratio between the magnolia flower extract and the phyllanthus emblica fruit extract is slightly adjusted, the free radical scavenging rates of Examples 1-3 show a certain degree of fluctuation trend. And when the mass ratio between the magnolia flower extract and the phyllanthus emblica fruit extract in Example 2 is 4:4, the composition undoubtedly has a more excellent free radical scavenging ability;

[0133] Further observing Examples 4-6, on the basis of Example 2, Examples 4-6 further introduce the lilac flower extract. And when the mass ratio between the magnolia flower extract, the phyllanthus emblica fruit extract, and the lilac flower extract is 4:4:4, the free radical scavenging ability of the composition is most significantly improved;

[0134] 2. It can be seen from Example 2 and Comparative Examples 1-2 that when the compositions of Comparative Examples 1-2 only contain the magnolia flower extract or the phyllanthus emblica fruit extract, the free radical scavenging rates of Comparative Examples 1-2 are only 33.39% and 34.38% respectively;

[0135] When the composition of Example 2 is a mixture with a mass ratio of 4:4 between the extract of Magnolia biondii Pamp. flowers and the extract of Phyllanthus emblica fruits, the free radical scavenging rate of Example 2 reaches 42.10%, which is much higher than that of any one of Comparative Examples 1 and 2;

[0136] It can be seen that there is a synergistic effect between the extract of Magnolia biondii Pamp. flowers and the extract of Phyllanthus emblica fruits, which significantly improves the free radical scavenging ability of the composition prepared in Example 2;

[0137] 3. It can be seen from Example 5, Comparative Examples 1-2, and Comparative Example 5 that when the compositions of Comparative Examples 1-2 contain only the extract of Magnolia biondii Pamp. flowers or the extract of Phyllanthus emblica fruits, the free radical scavenging rates of Comparative Examples 1-2 are only 33.39% and 34.38% respectively;

[0138] When the composition of Comparative Example 5 contains only the extract of Syringa oblata Lindl. flowers, the free radical scavenging rate of Comparative Example 5 is 38.62%; while when Example 5 is composed of the extract of Magnolia biondii Pamp. flowers, the extract of Phyllanthus emblica fruits, and the extract of Syringa oblata Lindl. flowers, its free radical scavenging rate reaches 50.61%. According to the free radical scavenging ability of the compositions of Comparative Examples 1-2 and Comparative Example 5, the theoretical value of the free radical scavenging rate of Example 5 should be (33.39% + 34.38% + 38.62%) / 3 = 35.46%;

[0139] It can be seen that the free radical scavenging rate of Example 5 exceeds its theoretical value, that is, the free radical scavenging ability of the composition prepared in Example 5 exceeds the simple superposition of Comparative Examples 1-2 and Comparative Example 5;

[0140] Thus, it can be seen that there is a synergistic effect between the extract of Magnolia biondii Pamp. flowers, the extract of Phyllanthus emblica fruits, and the extract of Syringa oblata Lindl. flowers;

[0141] Further observing Comparative Examples 3-4, it can be seen that when the extract of Magnolia biondii Pamp. flowers or the extract of Phyllanthus emblica fruits is used in combination with the extract of Syringa oblata Lindl. flowers, the free radical scavenging rate of Comparative Example 3 is 37.16%, and its ability to scavenge free radicals is higher than that of any one of Comparative Example 1 and Comparative Example 5. It can be seen that a relatively obvious synergistic effect is produced between the extract of Magnolia biondii Pamp. flowers and the extract of Syringa oblata Lindl. flowers in Comparative Example 3;

[0142] The free radical scavenging rate of Comparative Example 4 reaches 35.95%, which is between Comparative Example 2 and Comparative Example 5. It can be seen that the synergistic effect between the extract of Phyllanthus emblica fruits and the extract of Syringa oblata Lindl. flowers in Comparative Example 4 is very weak in the direction of free radical scavenging;

[0143] In summary, it can be known that there is a synergistic effect between the extract of Magnolia biondii Pamp. flowers and the extract of Phyllanthus emblica fruits;

[0144] 1. There is a synergistic effect between the extract of Magnolia biondii Pamp. flowers and the extract of Syringa oblata Lindl. flowers, but the synergistic effect is relatively poor compared with that between the extract of Phyllanthus emblica fruits and the extract of Magnolia biondii Pamp. flowers;

[0145] 2. The synergistic effect is weak when the lilac flower extract and the Phyllanthus emblica fruit extract are used together. However, when the magnolia flower extract is added to the composition, the synergistic effect of the three can be more fully exerted.

[0146] Application Example

[0147] An anti-wrinkle product has a formulation as shown in Table 6:

[0148] Table 6: Formulation Table of the Anti-Wrinkle Product

[0149]

[0150]

[0151] An anti-wrinkle product has a formulation referring to Table 6, and the specific preparation method is as follows:

[0152] Step 1: Phase A is preheated to 80 - 85 °C for dissolution, stirred and mixed evenly, and reserved.

[0153] Step 2: The raw materials in Phase B are added to the main pot in sequence and stirred evenly.

[0154] Step 3: Add Phase A and Phase C, stir evenly, and that's it.

[0155] The embodiments presented herein are only the implementation manners selected according to the combinations of all possible embodiments. The appended claims should not be limited by the embodiments illustrating the present invention. Some numerical ranges used in the claims include sub-ranges within them, and the changes in these ranges should also be covered by the appended claims.

Claims

1. An anti-wrinkle composition, characterized in that: The invention comprises a primula bicolor flower extract and a phyllanthus emblica fruit extract, wherein the mass ratio of the primula bicolor flower extract to the phyllanthus emblica fruit extract is 0.1-8:0.5-10.

2. The anti-wrinkle composition according to claim 1, characterized in that It also includes a lilac flower extract, wherein the mass ratio of the primrose extract, the emblica fruit extract and the lilac flower extract is 0.1-8:0.5-10:0.5-14.

3. A method for preparing the anti-wrinkle composition according to any one of claims 1 to 2, characterized in that: The extract of Primula ficus-indica flower and the extract of Emblica emblica fruit are mixed and dispersed to obtain an anti-wrinkle composition.

4. The method for preparing the anti-wrinkle composition according to claim 3, characterized in that: The extract of the flower of Bismarckia bicolor is extracted by drying and crushing the flower of Bismarckia bicolor, then heating, stirring and centrifuging in an alcohol solvent to obtain an intermediate, then immersing the intermediate in a fat-soluble solvent, stirring, extracting and centrifuging to obtain the flower of Bismarckia bicolor extract; The extracting method of the emblica fruit extract is as follows: drying and crushing the emblica fruit, then heating and stirring and centrifuging in an alcohol solvent to obtain an intermediate, then immersing the intermediate in a fat-soluble solvent, stirring, extracting and centrifuging to obtain the emblica fruit extract; The extraction method of the lilac flower extract comprises the following steps: drying and crushing the lilac flowers, then placing the lilac flowers in an alcohol solvent, heating, stirring, and centrifuging to obtain an intermediate, and then immersing the intermediate in a fat-soluble solvent, stirring, extracting, and centrifuging to obtain the lilac flower extract.

5. The method for preparing the anti-wrinkle composition according to claim 4, characterized in that: The fat-soluble solvent is selected from at least one of tri(ethylhexanoin)glyceryl, caprylic / capric triglyceride, octyldodecanol, coconut oil caprylate / caprate, ethylhexyl triazone, cetyl ethylhexanoate, and white meadowfoam seed oil.

6. Use of the anti-wrinkle composition according to any one of claims 1 to 2 in preparing cosmetics.

7. A cosmetic, characterized in that: Contains 0.1-20 wt % of the anti-wrinkle composition according to any one of claims 1-2.

8. The cosmetic according to claim 7, characterized in that: The dosage form of the cosmetic is a patch, ointment, liquid, spray, gel, emulsion or cream.

9. The cosmetic according to claim 7, characterized in that: The cosmetics are gel, essential oil, facial mask, facial cleanser, lipstick or blush.

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