Mixed grease for repairing sensitive skin as well as preparation method and application of mixed grease

Abstract

The invention provides mixed grease for repairing sensitive skin as well as a preparation method and application thereof. The mixed grease is prepared from the following raw materials: paeonia delavayi seeds, prinsepia utilis royle and camellia seeds. The prepared mixed grease has excellent anti-inflammatory, moisturizing and barrier repairing effects, the paeonia delavayi seeds, the prinsepia utilis royle and the camellia seeds have certain mutual cooperation and synergistic interaction effects on the effects, and maximization of the grease effect can be promoted through a specific extraction mode.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and in particular relates to a mixed oil for repairing sensitive skin, and a preparation method and application thereof. Background Art

[0002] The modern fast-paced lifestyle has brought many adverse effects on the skin. Long-term mental stress can lead to endocrine disorders in the human body, affecting the secretion of sebaceous glands and blood circulation of the skin. Imbalanced secretion of sebaceous glands may make the skin too oily or dry, while poor blood circulation will lead to insufficient nutrition supply to the skin, dull complexion, lack of luster, and reduced skin's self-repair ability.

[0003] Sensitive skin refers specifically to a highly reactive state of the skin under physiological or pathological conditions, which may manifest subjective symptoms such as burning, stinging, itching and tightness, accompanied by objective signs such as erythema, capillary dilation and desquamation. As the largest organ of the human body, the skin has important functions such as protecting the body from external aggression, regulating body temperature, and sensing external stimuli. Maintaining the healthy state of the skin, especially its soothing and moisturizing properties, is essential for maintaining the normal physiological function and overall health of the skin. Soothing and moisturizing skin can effectively resist external stimuli, reduce the risk of skin diseases, and improve the appearance and texture of the skin, making people look younger, healthier and more energetic. As consumers' awareness of skin care continues to increase, their demand for safe, effective and long-lasting skin care products that can simultaneously meet the dual effects of soothing and moisturizing is becoming more and more urgent. This market demand provides a strong impetus for the development of new soothing and moisturizing compositions, prompting researchers to continuously explore and innovate to develop better quality skin care products to meet the growing needs of consumers.

[0004] In view of the various adverse effects of environmental factors and lifestyle on the skin and the limitations of existing skin care products in soothing and moisturizing, the development of an efficient, stable and safe soothing and moisturizing composition is of great significance and has broad market prospects. Summary of the invention

[0005] In view of the deficiencies of the prior art, the object of the present invention is to provide a mixed oil for repairing sensitive skin and a preparation method and application thereof.

[0006] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0007] In a first aspect, the present invention provides a mixed oil for repairing sensitive skin, wherein raw materials for preparing the mixed oil include Yunnan peony seeds, green prunes and camellia seeds.

[0008] Yunnan peony seeds are rich in unsaturated fatty acids, such as linolenic acid and linoleic acid. Linolenic acid has the effects of lowering blood lipids, lowering cholesterol, promoting fat metabolism, liver cell regeneration, immunity, anti-allergic reaction, and anti-aging; linoleic acid inhibits cholesterol synthesis in the human body, anti-oxidation, anti-cancer, and prevents diabetes. These ingredients help maintain the health of the skin, enhance the skin's immune resistance, and reduce the damage of the external environment to the skin. In addition, Yunnan peony seeds are also rich in anthocyanins and other ingredients, which have strong anti-inflammatory, barrier repair and moisturizing effects on the skin.

[0009] The fruit of the Chinese prickly ash has a high oil content, which is rich in unsaturated fatty acids, polyphenols, flavonoids and other ingredients. These ingredients can form a protective film on the surface of the skin, effectively preventing the loss of moisture in the skin. This protective film is like a "barrier", reducing the contact of the skin with the dry environment outside, thereby achieving a good barrier skin care effect.

[0010] Camellia seeds mainly contain fatty acids, tea seed polysaccharides, polyphenols, flavonoids, saponins and other ingredients. Among them, fatty acids, tea polyphenols and camellia glycosides are its characteristic biologically active substances, which have the effects of lowering cholesterol, anti-aging and moisturizing.

[0011] The mixed oil prepared by the present invention has excellent anti-inflammatory, moisturizing and barrier repairing effects, and the Yunnan peony seeds, green thorn fruit and camellia seeds have certain mutual synergistic effects on the above effects.

[0012] Preferably, the raw materials for preparing the mixed oil include 4-6 parts of Yunnan peony seeds, 1-3 parts of utilitarian fruits and 1-2 parts of camellia seeds in parts by mass.

[0013] The mass proportion of Yunnan peony seeds can be selected as 4 parts, 4.2 parts, 4.4 parts, 4.6 parts, 4.8 parts, 5 parts, 5.2 parts, 5.4 parts, 5.6 parts, 5.8 parts, 6 parts, etc., the mass proportion of green thorn fruit can be selected as 1 part, 1.2 parts, 1.4 parts, 1.6 parts, 1.8 parts, 2 parts, 2.2 parts, 2.4 parts, 2.6 parts, 2.8 parts, 3 parts, etc., the mass proportion of camellia seeds can be selected as 1 part, 1.1 parts, 1.2 parts, 1.3 parts, 1.4 parts, 1.5 parts, 1.6 parts, 1.7 parts, 1.8 parts, 1.9 parts, 2 parts, etc., and other specific point values ​​within the above numerical range can be selected, so they will not be elaborated here one by one.

[0014] In a second aspect, the present invention provides a method for preparing the mixed oil for repairing sensitive skin according to the first aspect, the preparation method comprising:

[0015] (1) mixing the prepared raw material with the fermentation strain, fermenting, and drying to obtain a fermentation mixture;

[0016] (2) Subject the fermentation mixture to subcritical extraction to obtain crude oil;

[0017] (3) Filter the crude oil to obtain the product.

[0018] The specific extraction method of the present invention can not only extract fatty acid components in the mixed raw materials, but also extract small molecules such as polyphenols, flavonoids, and anthocyanins in the seed coat of the raw materials. These components synergistically promote the maximization of the effect. The method of mixing the preparation raw materials and then fermenting in this application has a better effect on the mixed oil obtained than the method of separately extracting the extracts and then physically mixing them. This extraction method can make the molecular connection between different oils closer, and the mixed oil obtained by this preparation method has better anti-inflammatory, moisturizing, and barrier repair effects than the oils obtained by traditional methods such as pressing and supercritical CO2 extraction.

[0019] Preferably, before mixing the preparation raw materials with the fermentation strain, it also includes mixing and pulverizing the preparation raw materials and sieving them.

[0020] Preferably, the fermentation strain includes Aspergillus niger and Bacillus pumilus with a viable cell number ratio of 1:(1 - 3).

[0021] The specific point values in (1 - 3) can all be selected as 1, 1.2, 1.4, 1.6, 1.8, 2, 2.2, 2.4, 2.6, 2.8, 3, etc. Other specific point values within the above numerical range can be selected, and they will not be elaborated here one by one.

[0022] Fermentation treatment with the mixed strain in the above specific ratio improves the extraction rate of the mixed oil, reduces the content of stearic acid and palmitic acid in saturated fatty acids, increases the content of linolenic acid in polyunsaturated fatty acids, changes the fatty acid composition ratio in the mixed oil, increases the content of small molecule functional substances in the mixed oil, and thus results in more excellent soothing, moisturizing, and barrier repair effects.

[0023] Preferably, the addition amount of the fermentation strain is 2×10 8 CFU / g - 5×10 8 CFU / g, for example 2×10 8 CFU / g, 2.2×10 8 CFU / g, 2.5×10 8 CFU / g, 2.8×10 8 CFU / g, 3×10 8 CFU / g, 3.2×10 8 CFU / g, 3.5×10 8 CFU / g, 3.8×10 8 CFU / g, 4×10 8 CFU / g, 4.2×108 CFU / g, 4.5×10 8 CFU / g, 4.8×10 8 CFU / g, 5×10 8 CFU / g, etc. Any other specific point values within the above numerical range can be selected and will not be elaborated one by one here.

[0024] Preferably, the temperature of the fermentation is 30 - 40°C, the time is 48 - 96 h, and the moisture content of the fermentation material is 30 - 50%.

[0025] The temperature can be selected as 30°C, 31°C, 32°C, 33°C, 34°C, 35°C, 36°C, 37°C, 38°C, 39°C, 40°C, etc., the time can be selected as 48 h, 52 h, 56 h, 60 h, 64 h, 68 h, 72 h, 76 h, 80 h, 84 h, 88 h, 92 h, 96 h, etc., and the moisture content can be selected as 30%, 32%, 34%, 36%, 38%, 40%, 42%, 44%, 46%, 48%, 50%, etc. Any other specific point values within the above numerical range can be selected and will not be elaborated one by one here.

[0026] Preferably, the extraction solvent used in the subcritical extraction is anhydrous ethanol and butane with a volume ratio of 1:(4 - 7).

[0027] Any specific point values in (4 - 7) can be selected as 4, 4.2, 4.5, 4.8, 5, 5.2, 5.5, 5.8, 6, 6.2, 6.5, 6.8, 7, etc. Any other specific point values within the above numerical range can be selected and will not be elaborated one by one here.

[0028] Preferably, the ratio of the fermentation mixture to the extraction solvent is 1 g:(2 - 10) mL.

[0029] Any specific point values in (2 - 10) can be selected as 2, 3, 4, 5, 6, 7, 8, 9, 10, etc. Any other specific point values within the above numerical range can be selected and will not be elaborated one by one here.

[0030] Preferably, the temperature of the subcritical extraction is 35 - 45°C, and the time for single extraction is 2 - 3 h.

[0031] The temperature can be selected as 35°C, 36°C, 37°C, 38°C, 39°C, 40°C, 41°C, 42°C, 43°C, 44°C, 45°C, etc., and the time can be selected as 2 h, 2.1 h, 2.2 h, 2.3 h, 2.4 h, 2.5 h, 2.6 h, 2.7 h, 2.8 h, 2.9 h, 3 h, etc. Any other specific point values within the above numerical range can be selected and will not be elaborated one by one here.

[0032] Preferably, the pressure of the subcritical extraction is 0.3 - 0.5 MPa, and the number of times is 2 - 3 times.

[0033] The pressure can be selected as 0.3 MPa, 0.32 MPa, 0.34 MPa, 0.36 MPa, 0.38 MPa, 0.4 MPa, 0.42 MPa, 0.44 MPa, 0.46 MPa, 0.48 MPa, 0.5 MPa, etc. Other specific point values within the above numerical range can be selected and will not be elaborated one by one here.

[0034] In a third aspect, the present invention provides an application of the mixed oil for repairing sensitive skin according to the first aspect in the preparation of a product having the effects of soothing, moisturizing or repairing the skin barrier.

[0035] Preferably, the mass percentage content of the mixed oil for repairing sensitive skin in the product is 0.1 - 0.5%, such as 0.1%, 0.11%, 0.12%, 0.13%, 0.14%, 0.15%, 0.16%, 0.18%, 0.2%, 0.25%, 0.3%, 0.35%, 0.4%, 0.45%, 0.5%, etc. Other specific point values within the above numerical range can be selected and will not be elaborated one by one here.

[0036] In a fourth aspect, the present invention provides an application of the mixed oil for repairing sensitive skin according to the first aspect in the preparation of an inflammatory factor inhibitor, an AQP3 gene activator, a LOR gene activator or a FLG gene activator.

[0037] Preferably, the inflammatory factors include TNF-α, IL-6 or NO.

[0038] According to the research results of the present invention, the mixed oil prepared by the present invention has a significant effect of inhibiting the secretion of inflammatory factors and has an activating effect on the AQP3 gene, the LOR gene and the FLG gene. Therefore, the prepared oil can be used as an in vitro preparation for basic scientific research, such as exploring the mechanism of action of physiological activities related to inflammatory reactions, or for the expression of barrier-activated genes.

[0039] Compared with the prior art, the present invention has the following beneficial effects:

[0040] The mixed oil prepared by the present invention has more excellent anti-inflammatory, moisturizing, and barrier repair effects. Moreover, Paeonia delavayi Franch. seeds, Prinsepia utilis Royle fruits, and Camellia seeds have a certain synergistic effect in the above effects, and a specific extraction method can maximize the effects. The oil obtained by this preparation method not only has a higher yield than that obtained by the traditional oil preparation method, retains some small molecule active substances (such as flavonoids, anthocyanins, etc.), but also has a higher content of unsaturated fatty acids, which changes the composition ratio of each fatty acid and has more excellent anti-inflammatory, moisturizing, and barrier repair effects. Detailed implementation manners

[0041] The technical solutions of the present invention will be further described below through specific implementation manners. Those skilled in the art should understand that the embodiments are only for helping to understand the present invention and should not be regarded as specific limitations on the present invention.

[0042] The sources of the active ingredients contained in the products involved in the following examples and comparative examples are as follows (only the active ingredients are shown, and the necessary auxiliary ingredients contained in other commercially available raw materials are not described in detail):

[0043] The preservation number of Aspergillus niger is CGMCC No. 3.3928, which is purchased from Beijing Center for Biological Preservation.

[0044] The preservation number of Bacillus pumilus is CMCC(B) 63202, which is purchased from Beijing Center for Biological Preservation.

[0045] Example 1

[0046] This example provides a mixed oil for repairing sensitive skin. The preparation raw materials of the mixed oil include 5 parts by mass of Paeonia delavayi Franch. seeds, 2 parts by mass of Prinsepia utilis Royle fruits, and 1.5 parts by mass of Camellia seeds.

[0047] Its preparation method is as follows:

[0048] (1) Mix and crush the preparation raw materials, sieve them through a 60-mesh sieve and then set aside. Inoculate Aspergillus niger (CGMCC No. 3.3928) and Bacillus pumilus (CMCC(B) 63202) with a viable cell number ratio of 1:2 into the crushed material with a moisture content of 40%, and the inoculation amount is 3.5×10 8 CFU / g. After solid-state fermentation at 37°C for 60 h, perform drying treatment to obtain the first mixture.

[0049] (2) Extract the first mixture with a subcritical extraction device. The ratio of the first mixture to the extraction solvent is 1 g:6 mL. The extraction solvent is anhydrous ethanol and butane with a volume ratio of 1:5. The extraction temperature is 40°C, the pressure is 0.4 Mpa, the single extraction time is 2 h, and the number of extractions is 2 times to obtain crude oil.

[0050] (3) Filter the crude oil through a 0.45 μm filter membrane to obtain the product.

[0051] Example 2

[0052] This example provides a mixed oil for repairing sensitive skin. The raw materials for preparing the mixed oil include 4 parts by mass of Paeonia delavayi Franch. seeds, 3 parts of Prinsepia utilis Royle fruits, and 1 part of Camellia oleifera Abel seeds.

[0053] Its preparation method is as follows:

[0054] (1) Mix and crush the raw materials for preparation, sieve through a 60-mesh sieve and set aside. Inoculate Aspergillus niger (CGMCC No. 3.3928) and Bacillus pumilus (CMCC(B) 63202) with a viable count ratio of 1:1 into the crushed material with a moisture content of 30%, and the inoculation amount is 2.5×10 8 CFU / g. After solid-state fermentation at 30 °C for 96 h, perform a drying treatment to obtain a first mixture.

[0055] (2) Extract the first mixture using a subcritical extraction device. The ratio of the first mixture to the extraction solvent is 1 g: 3 mL. The extraction solvent is anhydrous ethanol and butane with a volume ratio of 1:4. The extraction temperature is 35 °C, the pressure is 0.3 Mpa, the single extraction time is 2.5 h, and the number of extractions is 3 times to obtain crude oil.

[0056] (3) Filter the crude oil through a 0.45 μm filter membrane to obtain the product.

[0057] Example 3

[0058] This example provides a mixed oil for repairing sensitive skin. The raw materials for preparing the mixed oil include 6 parts by mass of Paeonia delavayi Franch. seeds, 1 part of Prinsepia utilis Royle fruits, and 2 parts of Camellia oleifera Abel seeds.

[0059] Its preparation method is as follows:

[0060] (1) Mix and crush the raw materials for preparation, sieve through a 60-mesh sieve and set aside. Inoculate Aspergillus niger (CGMCC No. 3.3928) and Bacillus pumilus (CMCC(B) 63202) with a viable count ratio of 1:3 into the crushed material with a moisture content of 50%, and the inoculation amount is 4.5×10 8 CFU / g. After solid-state fermentation at 40 °C for 48 h, perform a drying treatment to obtain a first mixture.

[0061] (2) Extract the first mixture using a subcritical extraction device. The ratio of the first mixture to the extraction solvent is 1 g: 8 mL. The extraction solvent is anhydrous ethanol and butane with a volume ratio of 1:7. The extraction temperature is 45 °C, the pressure is 0.5 Mpa, the single extraction time is 3 h, and the number of extractions is 2 times to obtain crude oil.

[0062] (3) Filter the crude oil through a 0.45 μm filter membrane to obtain the product.

[0063] Example 4

[0064] This example provides a blended oil for repairing sensitive skin. The difference from Example 1 is only that in step (2) of the preparation method, "the first mixture is pressed using a screw press to obtain crude oil".

[0065] The components and contents of the preparation raw materials remain unchanged, and other preparation steps remain unchanged.

[0066] Example 5

[0067] This example provides a blended oil for repairing sensitive skin. The difference from Example 1 is only that in step (2) of the preparation method, "the first mixture is extracted using a supercritical CO2 extraction device, with a CO2 flow rate of 20 L / h, an extraction temperature of 45 °C, a pressure of 8 Mpa, a single extraction time of 2.5 h, and the number of extractions is 2 times to obtain crude oil".

[0068] The components and contents of the preparation raw materials remain unchanged, and other preparation steps remain unchanged.

[0069] Example 6

[0070] This example provides a blended oil for repairing sensitive skin. The difference from Example 1 is only that the preparation raw materials of the blended oil include 3 parts by mass of Paeonia delavayi Franch. seeds, 4 parts by mass of Prinsepia utilis Royle fruits, and 0.5 part by mass of Camellia oleifera Abel seeds.

[0071] The components and contents of the preparation raw materials remain unchanged, and other preparation steps remain unchanged.

[0072] Example 7

[0073] This example provides a blended oil for repairing sensitive skin. The difference from Example 1 is only that the preparation raw materials of the blended oil include 7 parts by mass of Paeonia delavayi Franch. seeds, 0.5 part by mass of Prinsepia utilis Royle fruits, and 3 parts by mass of Camellia oleifera Abel seeds.

[0074] The components and contents of the preparation raw materials remain unchanged, and other preparation steps remain unchanged.

[0075] Example 8

[0076] This embodiment provides a blended oil for repairing sensitive skin. The difference from Embodiment 1 is only that in step (2), "the first mixture is extracted using a subcritical extraction device. The ratio of the first mixture to the extraction solvent is 1 g:6 mL. The extraction solvent is anhydrous ethanol and butane with a volume ratio of 1:8. The extraction temperature is 40°C, the pressure is 0.4 Mpa, the single extraction time is 2 h, and the number of extractions is 2 times to obtain crude oil".

[0077] The components and contents of the preparation raw materials remain unchanged, and other preparation steps remain unchanged.

[0078] Embodiment 9

[0079] This embodiment provides a blended oil for repairing sensitive skin. The difference from Embodiment 1 is only that in step (2), "the first mixture is extracted using a subcritical extraction device. The ratio of the first mixture to the extraction solvent is 1 g:6 mL. The extraction solvent is anhydrous ethanol and butane with a volume ratio of 1:3. The extraction temperature is 40°C, the pressure is 0.4 Mpa, the single extraction time is 2 h, and the number of extractions is 2 times to obtain crude oil".

[0080] The components and contents of the preparation raw materials remain unchanged, and other preparation steps remain unchanged.

[0081] Embodiment 10

[0082] This embodiment provides a blended oil for repairing sensitive skin. The difference from Embodiment 1 is only that in step (1), "the preparation raw materials are crushed, passed through a 60-mesh sieve and then reserved. Aspergillus niger (CGMCC No. 3.3928) is inoculated into the crushed material with a moisture content of 40%. The inoculation amount is 3.5×10 8 CFU / g. After solid-state fermentation at 37°C for 60 h, drying treatment is carried out to obtain the first mixture".

[0083] The components and contents of the preparation raw materials remain unchanged, and other preparation steps remain unchanged.

[0084] Embodiment 11

[0085] This embodiment provides a blended oil for repairing sensitive skin. The difference from Embodiment 1 is only that in step (1), "the preparation raw materials are crushed, passed through a 60-mesh sieve and then reserved. Bacillus pumilus (CMCC(B)63202) is inoculated into the crushed material with a moisture content of 40%. The inoculation amount is 3.5×10 8 CFU / g. After solid-state fermentation at 37°C for 60 h, drying treatment is carried out to obtain the first mixture".

[0086] The components and contents of the preparation raw materials remain unchanged, and other preparation steps remain unchanged.

[0087] Example 12

[0088] This example provides a mixed oil for repairing sensitive skin. The difference from Example 1 is only that step (1) is "crush the preparation raw materials, sieve them through a 60-mesh sieve and set aside, place them at 37 °C for 60 h and then perform drying treatment to obtain the first mixture".

[0089] The components and contents of the preparation raw materials remain unchanged, and other preparation steps remain unchanged.

[0090] Example 13

[0091] This example provides a mixed oil for repairing sensitive skin. The difference from Example 1 is only that step (1) is "mix and crush the preparation raw materials, sieve them through a 60-mesh sieve and set aside, inoculate Aspergillus niger (CGMCC No. 3.3928) and Bacillus pumilus (CMCC(B)63202) with a viable count ratio of 1:0.5 into the crushed material with a moisture content of 40%, and the inoculation amount is 3.5×10 8 CFU / g, perform solid-state fermentation at 37 °C for 60 h, and then perform drying treatment to obtain the first mixture".

[0092] The components and contents of the preparation raw materials remain unchanged, and other preparation steps remain unchanged.

[0093] Example 14

[0094] This example provides a mixed oil for repairing sensitive skin. The difference from Example 1 is only that step (1) is "mix and crush the preparation raw materials, sieve them through a 60-mesh sieve and set aside, inoculate Aspergillus niger (CGMCC No. 3.3928) and Bacillus pumilus (CMCC(B)63202) with a viable count ratio of 1:5 into the crushed material with a moisture content of 40%, and the inoculation amount is 3.5×10 8 CFU / g, perform solid-state fermentation at 37 °C for 60 h, and then perform drying treatment to obtain the first mixture".

[0095] The components and contents of the preparation raw materials remain unchanged, and other preparation steps remain unchanged.

[0096] Example 15

[0097] This example provides a mixed oil for repairing sensitive skin. The difference from Example 1 is only that the preparation method is different.

[0098] The preparation method is as follows: separately prepare the oils from Paeonia delavayi Franch. seeds, Prinsepia utilis Royle fruits and Camellia seeds with reference to the preparation method of Example 1. The mass ratio of Paeonia delavayi Franch. seeds, Prinsepia utilis Royle fruits and Camellia seeds is the same as that in Example 1, and then physically mix the prepared Paeonia delavayi Franch. seed oil, Prinsepia utilis Royle fruit oil and Camellia seed oil to obtain it.

[0099] Comparative Example 1

[0100] This comparative example provides a mixed oil for repairing sensitive skin. The difference from Example 1 is only that the preparation raw materials do not contain Paeonia delavayi Franch. seeds, and the reduced mass is proportionally distributed to the masses of Prinsepia utilis Royle and Camellia seeds, while other components and contents remain unchanged.

[0101] The preparation method refers to Example 1.

[0102] Comparative Example 2

[0103] This comparative example provides a mixed oil for repairing sensitive skin. The difference from Example 1 is only that the preparation raw materials do not contain Prinsepia utilis Royle, and the reduced mass is proportionally distributed to the masses of Paeonia delavayi Franch. seeds and Camellia seeds, while other components and contents remain unchanged.

[0104] The preparation method refers to Example 1.

[0105] Comparative Example 3

[0106] This comparative example provides a mixed oil for repairing sensitive skin. The difference from Example 1 is only that the preparation raw materials do not contain Camellia seeds, and the reduced mass is proportionally distributed to the masses of Paeonia delavayi Franch. seeds and Prinsepia utilis Royle, while other components and contents remain unchanged.

[0107] The preparation method refers to Example 1.

[0108] Test Example 1

[0109] Anti-inflammatory and soothing effect

[0110] Using LPS-induced mouse macrophage RAW264.7 as an anti-inflammatory efficacy test model, a blank control group, an LPS-induced group, a positive control group (dexamethasone, 120 μg / mL), and a sample group were set up. The concentrations of the samples in each example and comparative example were all 120 μg / mL. After incubating in a 37°C, 5% CO2 incubator for 24 h, the cell supernatants of each group were collected, and then the contents of inflammatory factors TNF-α, IL-6, and NO in the collected cell supernatants were detected according to the ELISA kit detection method. The experimental results were analyzed by data processing software to evaluate the inhibition rate of the mixed oil on the expression of inflammatory factors. The inhibition rate calculation formula is:

[0111]

[0112] Table 1

[0113]

[0114] As can be seen from the data in Table 1, the mixed oil and fat prepared by the present invention has good anti-inflammatory effects, can inhibit the expression of inflammatory factors, and Paeonia delavayi Franch. seeds, Prinsepia utilis Royle fruits and Camellia seeds have a certain cooperative effect in the above effects, and none of the three can be missing. The pre-fermentation treatment can improve the technical effect of extraction. The co-fermentation of Aspergillus niger and Bacillus pumilus can maximize the fermentation effect, and neither of the two strains can be missing. The effect of subcritical extraction is better than other extraction effects.

[0115] Test Example 2

[0116] Moisturizing effect

[0117] HaCaT cells in the logarithmic growth phase with good morphology were selected and inoculated into 24-well plates at 1×10 5 cells / well and incubated in an incubator for 24 h. A blank control group, a positive control group (calcium chloride, 120 μg / mL) and a sample group were set up. The concentrations of the samples in the examples and comparative examples were both 120 μg / mL. The cells were treated with the samples and then co-incubated in a 37 °C, 5% CO2 incubator for another 24 h. Then the RNA of each group was extracted, the RNA content was measured, cDNA was reverse transcribed, and a fluorescence quantitative PCR experiment was carried out. The experimental results were analyzed by data processing software to evaluate the activation effect of the mixed oil and fat on the expression of aquaporin AQP3 mRNA. The activation rate calculation formula is:

[0118]

[0119] Table 2

[0120] AQP3 activation rate Positive control group 16.23％ Example 1 74.85％ Example 2 73.59％ Example 3 72.64％ Example 4 41.32％ Example 5 43.16％ Example 6 36.85％ Example 7 40.56％ Example 8 47.21％ Example 9 49.03％ Example 10 45.10％ Example 11 41.48％ Example 12 34.93％ Example 13 50.31％ Example 14 47.83％ Example 15 49.60％ Comparative example 1 36.84％ Comparative example 2 32.85％ Comparative example 3 21.05％

[0121] As can be seen from the data in Table 2, the mixed oil and fat prepared by the present invention has good moisturizing effects, and Paeonia delavayi Franch. seeds, Prinsepia utilis Royle fruits and Camellia seeds have a certain cooperative effect in the above effects, and none of the three can be missing. The pre-fermentation treatment can improve the technical effect of extraction. The co-fermentation of Aspergillus niger and Bacillus pumilus can maximize the fermentation effect, and neither of the two strains can be missing. The effect of subcritical extraction is better than other extraction effects.

[0122] Test Example 3

[0123] Barrier repair

[0124] HaCaT cells in the logarithmic growth phase with good morphology were selected and inoculated into 24-well plates at 1×10 5Cells / well were incubated in an incubator for 24 h. A control group and a sample group were set up. In the sample group, the concentrations of the samples in the examples and comparative examples were all 120 μg / mL. The cells were treated with the samples and then co-incubated in an incubator at 37 °C and 5% CO2 for another 24 h. RNA was extracted from each group, the RNA content was measured, cDNA was reverse transcribed, and the experimental results were analyzed by data processing software in the fluorescence quantitative PCR experiment to evaluate the activation effect of the mixed oil on the mRNA expression of filaggrin (FLG) and loricrin (LOR) related to barrier repair. The activation rate calculation formula is:

[0125]

[0126] Table 3

[0127]

[0128]

[0129] It can be seen from the data in Table 3 that the mixed oil prepared by the present invention has a good barrier repair effect, can significantly activate the expression of FLG and LOR mRNA, and Paeonia delavayi Franch. seeds, Prinsepia utilis Royle and Camellia seeds have a certain cooperative effect on the above effects, and none of them can be missing. The pretreatment before fermentation can improve the technical effect of extraction. The co-fermentation of Aspergillus niger and Bacillus pumilus can maximize the fermentation effect, and neither of the two strains can be missing. The effect of subcritical extraction is better than other extraction effects.

[0130] Test Example 4

[0131] The detection method of fatty acid content refers to the national standard GB5009.168—2016, Determination of Fatty Acids in Foods (Normalization Method).

[0132] Total flavonoid detection method: 0.5 mL of the test solution and rutin reference solution were taken into a colorimetric tube respectively, 0.2 mL of 5% NaNO2 was added to each, mixed well, and after standing for 6 min, 0.2 mL of 1% Al(NO3)3 was added to each and mixed well, then stood for 6 min, and then 1 mL of 4% NaOH was added respectively. After standing at room temperature for 15 min, the absorbance was measured at a wavelength of 500 nm. The standard curve of the rutin reference solution was drawn, and the absorbance of the test sample was substituted into the standard curve to calculate the total flavonoid content.

[0133] The method for detecting anthocyanins is as follows: Prepare an anthocyanin standard solution with a concentration of 0.5 mg / mL. Respectively pipette 0.1, 0.2, 0.3, 0.4, and 0.5 mL into colorimetric tubes, and add methanol solution to each tube until the volume reaches 1.0 mL. Then, add 6 mL of n-butanol-hydrochloric acid solution and 0.2 mL of ammonium ferric sulfate solution with a mass fraction of 2% to both the standard solution and the test solution. After shaking well, heat in a boiling water bath for 40 min, and then quickly cool. Measure the absorbance of the anthocyanin standard solution and the test solution at a wavelength of 530 nm, plot the anthocyanin standard curve, and then calculate the anthocyanin content of the test solution.

[0134] Table 4

[0135] Example 1 Example 2 Example 3 Example 12 Oleic acid (%) 33.2 32.9 32.1 37.6 Linoleic acid (%) 25.6 24.2 24.4 27.6 Linolenic acid (%) 18.4 18.6 19.2 14.4 Total flavonoids (%) 0.54 0.49 0.51 0.32 Anthocyanin (%) 1.44 1.21 1.17 0.86

[0136] As can be seen from the data in Table 4, the oleic acid, linoleic acid, linolenic acid, total flavonoids, and anthocyanin contents of the mixed oil obtained by the specific preparation method of the present invention are all relatively high. The oleic acid and linoleic acid contents of Example 12 (without microbial fermentation) are slightly higher than those of Examples 1-3, while the linolenic acid content is lower than that of Examples 1-3, and the contents of total flavonoids and anthocyanins are lower than those of Examples 1-3. It can be seen that microbial fermentation changes the component ratio of the mixed oil, thereby enhancing the soothing, moisturizing, and barrier repair effects of the mixed oil.

[0137] The applicant declares that the present invention uses the above examples to illustrate a mixed oil for repairing sensitive skin, its preparation method, and application. However, the present invention is not limited to the above examples, that is, it does not mean that the present invention must rely on the above examples to be implemented. Those skilled in the art should understand that any improvement of the present invention, the equivalent substitution of each raw material of the product of the present invention, the addition of auxiliary components, and the selection of specific methods, etc., all fall within the protection scope and the disclosure scope of the present invention.

[0138] The preferred embodiments of the present invention have been described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, various simple modifications can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0139] In addition, it should be noted that in the various specific technical features described in the above specific embodiments, they can be combined in any suitable manner without conflict. To avoid unnecessary repetition, the present invention will not separately describe various possible combination methods.

Claims

1. A mixed oil for repairing sensitive skin, characterized in that: The raw materials for preparing the mixed oil include Yunnan peony seeds, green thorn fruit and camellia seeds.

2. The mixed oil for repairing sensitive skin according to claim 1, characterized in that: The raw materials for preparing the mixed oil include 4-6 parts of Yunnan peony seeds, 1-3 parts of prickle fruits and 1-2 parts of camellia seeds in parts by weight.

3. The method for preparing the mixed oil for repairing sensitive skin according to claim 1 or 2, characterized in that: The preparation method comprises: (1) mixing the prepared raw material with the fermentation strain, fermenting, and drying to obtain a fermentation mixture; (2) extracting the fermentation mixture using subcritical water to obtain crude oil; (3) Filter the crude oil to obtain.

4. The method for preparing the mixed oil for repairing sensitive skin according to claim 3, characterized in that: The fermentation strains include Aspergillus niger and Bacillus pumilus with a live bacterial count ratio of 1:(1-3).

5. The method for preparing the mixed oil for repairing sensitive skin according to claim 3, characterized in that: The amount of the fermentation strain added was 2×10 8 CFU / g-5×10 8 CFU / g.

6. The method for preparing the mixed oil for repairing sensitive skin according to claim 3, characterized in that: The fermentation temperature is 30-40° C., the fermentation time is 48-96 hours, and the moisture content of the fermented material is 30-50%.

7. The method for preparing the mixed oil for repairing sensitive skin according to claim 3, characterized in that: The extraction solvent used in the subcritical extraction is anhydrous ethanol and butane in a volume ratio of 1:(4-7); The ratio of the fermentation mixture to the extraction solvent is 1 g: (2-10) mL.

8. The method for preparing the mixed oil for repairing sensitive skin according to claim 3, characterized in that: The temperature of the subcritical extraction is 35-45° C., the time of a single extraction is 2-3 hours, the number of times is 2-3 times, and the extraction pressure is 0.3-0.5 MPa.

9. Use of the mixed oil for repairing sensitive skin according to claim 1 or 2 in preparing products with soothing, moisturizing or barrier repairing effects.

10. Use of the mixed oil for repairing sensitive skin according to claim 1 or 2 in the preparation of an inflammatory factor inhibitor, an AQP3 gene activator, a LOR gene activator or a FLG gene activator.

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