After-sun repair composition for improving mood, application, cosmetic and preparation method of after-sun repair composition

Abstract

The invention discloses an after-sun repair composition for improving emotion, application, cosmetics and a preparation method of the cosmetics. The after-sun repair composition is prepared from alfalfa extract and zanthoxylum armatum fruit extract, the mass ratio of the alfalfa extract to the zanthoxylum armatum fruit extract is (0.1-2%): (0.1-2%). According to the after-sun repair composition for improving the emotion and the application thereof, the cosmetic and the preparation method of the after-sun repair composition, through the synergistic effect of the alfalfa extract and the zanthoxylum armatum fruit extract, the after-sun repair composition can meet the requirements for water replenishing, moisturizing, relieving and anti-inflammation, and meanwhile the effect of improving the emotion is achieved by reducing the cortisol level in skin cells and increasing release of dopamine. The effects of improving the mood and reducing the damage to the skin caused by the negative mood generated by the appearance change of the sun-dried skin are achieved.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and relates to a cosmetic, in particular to a post-sun repair composition for enhancing mood, its application, a cosmetic and a preparation method thereof. Background Art

[0002] The ultraviolet rays contained in sunlight mainly have wavelengths in the range of 290nm - 400nm. Among them, medium-wave ultraviolet rays (UVB: 280nm - 315nm) have short wavelengths, high frequencies, and strong photon energies. A relatively small dose can cause capillary dilation and erythema. Long-term exposure can cause hyperplasia of keratinocytes, leading to thickening, roughness, and wrinkles of the skin. Large doses or long-term exposure often cause sunburn on the skin surface, manifested as skin redness, edema, or blisters, with a burning and painful sensation. Pigmentation spots may remain after recovery. The penetration ability of long-wave ultraviolet rays (UVA: 315 - 400nm) is relatively strong and can reach the dermis layer, damaging collagen and elastic fibers, thus causing skin relaxation and aging, accelerating the formation of wrinkles. Moreover, long-wave ultraviolet rays can promote the formation of skin melanin, resulting in skin pigmentation and inducing or aggravating freckles, melasma, etc. Long-term ultraviolet irradiation may cause damage to the DNA structure of the skin, leading to a decline in skin immune function and ultimately forming precancerous lesions or skin cancer.

[0003] As the largest organ of the human body, in addition to its protective and immune functions, the nervous and endocrine functions are also crucial for skin health. The skin and the nervous system both originate from the ectoderm and communicate with immune cells by releasing neurotrophic factors and neuropeptides, thereby affecting skin immune responses. The skin is both a target organ for the action of various hormones and an endocrine organ that secretes various hormones, and has the same function as the hypothalamic-pituitary-adrenal (HPA) axis. When the brain is stimulated by the outside world such as stress, the hypothalamus will secrete hormones, some of which will have negative effects, such as cortisol (stress hormone). These hormones will regulate the autonomic nerves to make people enter a stress state and cooperate with other structures of the limbic system to produce a series of physical reactions. Long-term elevation of cortisol levels will inhibit the migration and differentiation of skin keratinocytes, leading to inflammatory reactions and thus preventing the renewal of the skin stratum corneum.

[0004] Skin redness and swelling caused by sun exposure and pigmentation affect the appearance of the skin and easily lead to mood swings such as depression, anxiety, and melancholy. When in a low mood or anxiety, the release of catecholamine substances in the human body increases, thereby increasing adrenaline secretion, causing arteriolar vasoconstriction, and suddenly reducing the blood supply to the skin. At the same time, epithelial cells synthesize too much melanin and deposit it on the skin surface, making the skin become dull or even sallow. A negative emotional state will cause physiological changes in the skin, exacerbating the symptoms and thus forming a vicious cycle. Therefore, it is also crucial to enhance mood during the post-sun repair process. However, there is currently no relevant research report on post-sun repair and mood enhancement.

[0005] First of all, in the current field related to post-sun repair, such as Chinese invention patent CN201510868359.5, it can only play a role in simple moisturizing, suppressing inflammation, and promoting skin repair, and cannot relieve the anxiety caused by the appearance of post-sun skin. A negative emotional state will lead to physiological changes in the skin, aggravating the symptoms, thus forming a vicious cycle.

[0006] Secondly, there is relatively little research on current mood-enhancing compositions. Some solutions, such as Chinese invention patent CN202311856514.2, achieve the effects of relieving anxiety and improving sleep quality by using fragrant essential oils. Individual differences in personal olfactory sensitivity will affect likes and dislikes of odors, and aromatic essential oils have strong volatility. After inhalation by rhinitis patients, it may induce acute attacks of rhinitis, and frequent inhalation may also lead to aggravation of the rhinitis condition. Therefore, the method of enhancing mood through olfaction has limited applicability.

[0007] Finally, in the existing research on mood-enhancing compositions, such as Chinese invention patent CN202211516070.3, it only contains single-effect components and is vulnerable to storage and usage conditions, resulting in inactivation of the active components and a significant reduction in the usage effect. The present invention adds multiple active components to form a composition, and through the synergistic effect of reducing cortisol levels and promoting dopamine release, even if some components are inactivated, the mood-enhancing effect of the composition can still be ensured.

[0008] In view of this, there is an urgent need to design a new mood-enhancing composition today to overcome at least some of the above-mentioned defects existing in the existing mood-enhancing compositions. Summary of the Invention

[0009] The present invention provides a post-sun repair composition for enhancing mood, its application, a cosmetic, and a preparation method thereof. While meeting the requirements of the post-sun repair composition for moisturizing and soothing anti-inflammation, it can elevate mood and reduce the harm caused by negative emotions generated by post-sun skin appearance changes to the skin by reducing cortisol levels in skin cells and increasing the release of dopamine.

[0010] To solve the above technical problems, according to one aspect of the present invention, the following technical solution is adopted:

[0011] A post-sun repair composition for enhancing mood, the post-sun repair composition comprising: alfalfa extract and zanthoxylum schinifolium fruit extract; the mass ratio of the alfalfa extract to the zanthoxylum schinifolium fruit extract is (0.1 - 2%):(0.1 - 2%).

[0012] As an implementation manner of the present invention, the mass ratio of the alfalfa extract to the zanthoxylum schinifolium fruit extract is (0.1 - 1%):(0.1 - 1%).

[0013] According to another aspect of the present invention, the following technical solution is adopted: The application of the above-mentioned post-sun repair composition for improving mood in the preparation of cosmetics with post-sun repair efficacy.

[0014] According to still another aspect of the present invention, the following technical solution is adopted: A cosmetic, which comprises the above-mentioned post-sun repair composition.

[0015] As an embodiment of the present invention, the cosmetic is essence, cream, lotion, freeze-dried powder or skin care lotion.

[0016] As an embodiment of the present invention, the cosmetic is essence; based on 100 parts by mass of the essence, it comprises 0.2 - 4 parts of the post-sun repair composition, 1.2 - 82 parts of raw material combination A, 0.2 - 13 parts of raw material combination B, 0.1 - 5 parts of raw material combination C and 0.11 - 6 parts of raw material combination D;

[0017] Wherein: The post-sun repair composition comprises 0.1 - 2 parts of alfalfa extract and 0.1 - 2 parts of zanthoxylum armatum fruit extract;

[0018] The raw material combination A comprises 0.1 - 25 parts of thickener A, 0.1 - 30 parts of skin conditioner A and 1 - 30 parts of humectant A;

[0019] The raw material combination B comprises 0.1 - 10 parts of emulsifier and 0.1 - 5 parts of skin conditioner B;

[0020] The raw material combination C comprises 0.1 - 5 parts of pH regulator;

[0021] The raw material combination D comprises 0.1 - 5 parts of fragrance and 0.01 - 1 part of preservative; the above parts are all parts by mass.

[0022] As an embodiment of the present invention, the raw material combination A further comprises water, and the volume of the water is such that the weight of the essence reaches 100 parts;

[0023] The thickener A comprises at least one of acrylic acid (ester) / C10-30 alkyl acrylate cross-linked polymer, sodium acrylate / acryloyldimethyltaurine copolymer, polyacrylamide, xanthan gum, cyclopentasiloxane, propylene glycol, cetearyl alcohol, carbomer, laureth-7, cetyl alcohol, stearyl alcohol and hydroxyethyl cellulose;

[0024] The skin conditioner A and the skin conditioner B each independently comprise at least one of niacinamide, isocetane, olive oil, sodium hyaluronate, lauryl alcohol, shea butter, allantoin, bisabolol, butylene glycol, squalane, panthenol, lactic acid, tocopherol and dipotassium glycyrrhizinate.

[0025] As an embodiment of the present invention, the moisturizer A comprises at least one of hyaluronic acid, allantoin, glycerol, ceramide, sodium lactate, butylene glycol, hydrogenated grape seed oil, olive oil and shea butter;

[0026] The emulsifier comprises at least one of glyceryl stearate citrate, cetearyl glucoside (and) cetearyl alcohol, behenyl alcohol, behenyl trimethyl ammonium methyl sulfate, coconut oil glycerides and sorbitan oleate;

[0027] The pH regulator comprises at least one of arginine and lactic acid; the fragrance comprises essence; the preservative comprises at least one of phenoxyethanol, ethylhexylglycerin and pentanediol.

[0028] According to another aspect of the present invention, the following technical solution is adopted: a preparation method of the above cosmetic, the preparation method comprising:

[0029] Step a, obtaining an aqueous phase: placing water in a container with a stirring device, fully wetting and mixing other parts of the raw material combination A except water and then adding them into the water, fully stirring until completely dissolved, and heating;

[0030] Step b, obtaining an oil phase: mixing and heating the raw material combination B, and stirring until completely dissolved;

[0031] Step c, emulsification step: adding the mixture of step b into the aqueous phase of step a, and stirring and homogenizing for a set time;

[0032] Step d, adding the raw material combination C, stirring and homogenizing for a set time; stirring and cooling to 40-45 °C;

[0033] Step e, adding the post-sun repair composition, stirring and homogenizing for a set time;

[0034] Step f, waiting for the temperature to be stirred and cooled to 35-38 °C, adding the raw material combination D, stirring and homogenizing for a set time, and then the required post-sun repair essence is obtained.

[0035] As an embodiment of the present invention, in the emulsification step of step c, the mixture of step b is added into the aqueous phase of step a, and stirred and homogenized for 3-10 minutes;

[0036] In step d, raw material combination C is added, and it is stirred at a speed of 250 - 350 rpm for 3 - 5 minutes and homogenized at a speed of 2500 - 3500 rpm for 3 - 10 minutes; the temperature is reduced to 40 - 45 °C during stirring;

[0037] In step e, a post - sun repair composition is added, and it is stirred at a speed of 250 - 350 rpm for 3 - 5 minutes and homogenized at a speed of 2500 - 3500 rpm for 3 - 10 minutes;

[0038] In step f, when the temperature is reduced to 35 - 38 °C during stirring, raw material combination D is added, and it is stirred at 250 - 350 rpm and homogenized at 2500 - 3500 rpm for 3 - 10 minutes; it is filtered and sterilized before filling, and the bacterial content is detected and quality control is carried out on toxins, immunogenicity, etc., thus obtaining the required post - sun repair essence.

[0039] The beneficial effects of the present invention are as follows: The post - sun repair composition, application, cosmetics and preparation method for enhancing mood proposed by the present invention, through the synergistic effect of Medicago sativa extract and Zanthoxylum armatum fruit extract, can, while meeting the requirements of the post - sun repair composition for moisturizing, soothing and anti - inflammatory, reduce the cortisol level in skin cells and increase the release of dopamine, so as to achieve the effect of enhancing mood and reducing the damage caused by negative emotions generated by post - sun skin appearance changes to the skin.

[0040] The present invention is different from enhancing mood through aromatic smell; the composition is applied to the skin, and by reducing the cortisol level in cells and increasing the release of dopamine, the mood - enhancing effect is achieved, completely avoiding the irritation of aromatic components to the sense of smell and meeting the mood - enhancing needs of people sensitive to smell.

[0041] Through the synergistic effect of Medicago sativa extract and Zanthoxylum armatum fruit extract, the present invention ensures that the efficacy of the composition can be stably exerted under various usage conditions. Even if some components are inactivated, the mood - enhancing efficacy of the composition can still be guaranteed, increasing the stability and usability of the composition, reducing the compatibility difficulty, and the combined use is significantly better than the effect of each component used alone.

[0042] In a usage scenario of the present invention, the composition of the present invention can not only, through the synergistic effect of Medicago sativa extract and Zanthoxylum armatum fruit extract, inhibit the inflammatory reaction through multi - target synergy, increase the expression of loricrin and aquaporin - 3 (AQP3), promote the proliferation, differentiation of keratinocytes and the synthesis of extracellular matrix (ECM), and jointly achieve the effect of enhancing the skin barrier function and accelerating the repair of post - sun skin. In particular, Medicago sativa extract can reduce the cortisol level in skin cells, and Zanthoxylum armatum fruit extract can increase the release of dopamine. The two work together to regulate neurotransmitters in multiple ways, and jointly achieve the effect of enhancing mood and preventing a series of negative effects on the skin caused by neurotransmitters that produce negative emotions. Description of the Drawings

[0043] Figure 1 The figure is a flowchart of the method for preparing a cosmetic in an embodiment of the present invention. Detailed Description of the Invention

[0044] The preferred embodiments of the present invention will be described in detail below with reference to the drawings.

[0045] In order to further understand the present invention, the preferred embodiments of the present invention will be described below in conjunction with the embodiments. However, it should be understood that these descriptions are only for further explaining the features and advantages of the present invention, rather than limiting the claims of the present invention.

[0046] The description of this part only focuses on several typical embodiments, and the present invention is not limited to the scope described in the embodiments. The mutual replacement of the same or similar prior art means and some technical features in the embodiments is also within the scope of the description and protection of the present invention.

[0047] Unless otherwise specified, implied from the context or in accordance with the convention of the prior art, all parts and percentages in this application are based on weight, and the test and characterization methods used are synchronized with the filing date of this application. Where applicable, any patents, patent applications or published content referred to in this application are incorporated herein by reference in their entirety, and their equivalent family patents are also incorporated by reference, particularly the definitions of synthetic techniques, products and processing designs, polymers, comonomers, initiators or catalysts, etc. in the art disclosed in these documents. If the definition of a specific term disclosed in the prior art is inconsistent with any definition provided in this application, the definition of the term provided in this application shall prevail.

[0048] The numerical ranges in this application are approximate values, so unless otherwise specified, they may include values outside the ranges. The numerical ranges include all values from the lower limit value to the upper limit value increasing by 1 unit, provided that there is at least a 2-unit interval between any lower value and any higher value. For example, if it is stated that a component, physical or other property (such as molecular weight, melt index, etc.) is from 100 to 1000, it means that all individual values are explicitly listed, such as 100, 101, 102, etc., and all sub-ranges, such as 100 to 166, 155 to 170, 198 to 200, etc. For ranges containing values less than 1 or containing fractions greater than 1 (such as 1.1, 1.5, etc.), 1 unit is appropriately regarded as 0.0001, 0.001, 0.01 or 0.1. For ranges containing single-digit numbers less than 10 (such as 1 to 5), 1 unit is usually regarded as 0.1. These are just specific examples of what is intended to be expressed, and all possible combinations of the values between the lowest and highest values listed are considered to be clearly recited in this application. It should also be noted that the terms "first", "second", etc. in this article do not limit the order of precedence, but are only used to distinguish substances with different structures.

[0049] When used with respect to chemical compounds, unless explicitly stated, the singular includes all isomeric forms and vice versa (e.g., "hexane" includes individually or collectively all isomers of hexane). Additionally, unless explicitly stated, nouns described by "a", "an" or "the" also include their plural forms.

[0050] The terms "comprising", "including", "having" and their derivatives do not exclude the presence of any other components, steps or processes, and are independent of whether these other components, steps or processes are disclosed in this application. To eliminate any doubt, unless explicitly stated, all compositions using the terms "comprising", "including", or "having" in this application may contain any additional additives, excipients or compounds. In contrast, except for those necessary for the operating performance, the term "consisting essentially of" excludes any other components, steps or processes from the scope described below any such term. The term "consisting of" does not include any component, step or process not specifically described or listed. Unless explicitly stated, the term "or" refers to the individual members listed or any combination thereof.

[0051] The expressions of the steps in each embodiment in the specification are only for convenience of description, and the implementation manner of this application is not limited by the order of step implementation.

[0052] The present invention discloses a post-sun repair composition for enhancing mood, and the post-sun repair composition includes: alfalfa extract and zanthoxylum schinifolium fruit extract; the mass ratio of the alfalfa extract to the zanthoxylum schinifolium fruit extract is (0.1-2%):(0.1-2%). In an embodiment of the present invention, the mass ratio of the alfalfa extract to the zanthoxylum schinifolium fruit extract is (0.1-1%):(0.1-1%).

[0053] The present invention also discloses the application of the above-mentioned post-sun repair composition for enhancing mood in the preparation of cosmetics with post-sun repair efficacy.

[0054] The present invention further discloses a cosmetic, and the cosmetic includes the above-mentioned post-sun repair composition. The cosmetic can be a serum, a cream, a lotion, a freeze-dried powder or a skin care lotion.

[0055] In an embodiment of the present invention, the cosmetic is a serum; based on 100 parts by weight of the serum, it includes 0.2-4 parts of the post-sun repair composition, 1.2-82 parts of raw material combination A, 0.2-13 parts of raw material combination B, 0.1-5 parts of raw material combination C and 0.11-6 parts of raw material combination D. Among them: the post-sun repair composition includes 0.1-2 parts of alfalfa extract and 0.1-2 parts of zanthoxylum schinifolium fruit extract;

[0056] The raw material combination A includes 0.1-25 parts of thickener A, 0.1-30 parts of skin conditioner A and 1-30 parts of humectant A; the raw material combination B includes 0.1-10 parts of emulsifier and 0.1-5 parts of skin conditioner B; the raw material combination C includes 0.1-5 parts of pH regulator; the raw material combination D includes 0.1-5 parts of fragrance and 0.01-1 part of preservative.

[0057] The raw material combination A may further include water, and the volume of the water is such that the weight of the serum reaches 100 parts. The thickener A includes at least one of acrylic (ester) / C10-30 alkanol acrylate cross-linked polymer, sodium acrylate / acryloyldimethyltaurine copolymer, polyacrylamide, xanthan gum, cyclopentasiloxane, propylene glycol, cetearyl alcohol, carbomer, laureth-7, cetyl alcohol, stearyl alcohol and hydroxyethyl cellulose. Each of the skin conditioner A and the skin conditioner B independently includes at least one of niacinamide, isocetane, olive oil, sodium hyaluronate, lauryl alcohol, shea butter, allantoin, bisabolol, butylene glycol, squalane, panthenol, lactic acid, tocopherol and dipotassium glycyrrhizinate.

[0058] The moisturizer A includes at least one of hyaluronic acid, allantoin, glycerin, ceramide, sodium lactate, butanediol, hydrogenated grape seed oil, olive oil, and shea butter. The emulsifier includes at least one of glyceryl stearate citrate, cetearyl glucoside (and) cetearyl alcohol, behenyl alcohol, behenyl trimethylammonium methyl sulfate, coconut oil glycerides, and sorbitan oleate. The pH regulator includes at least one of arginine and lactic acid; the fragrance includes essence; the preservative includes at least one of phenoxyethanol, ethylhexylglycerin, and pentylene glycol.

[0059] The present invention also discloses a preparation method of the above-mentioned cosmetic (serum), Figure 1 which is a flowchart of the cosmetic preparation method in an embodiment of the present invention; please refer to Figure 1 and the preparation method includes:

[0060]

Step a

[0061]

Step b

[0062]

Step c

[0063] In an embodiment of the present invention, the mixture in step b is added to the aqueous phase in step a, and stirred and homogenized for 3 to 10 minutes.

[0064]

Step d

[0065] In an embodiment of the present invention, in step d, add the raw material combination C, stir at a speed of 250 - 350 rpm for 3 to 5 minutes, homogenize at a speed of 2500 - 3500 rpm for 3 to 10 minutes; stir and cool down to 40 - 45 °C.

[0066]

Step e

[0067] In an embodiment of the present invention, add the after - sun repair composition, stir at a speed of 250 - 350 rpm for 3 to 5 minutes, homogenize at a speed of 2500 - 3500 rpm for 3 to 10 minutes.

[0068]

Step f

[0069] In one embodiment of the present invention, it is stirred and cooled to 35-38°C, raw material combination D is added, and it is stirred at a speed of 250-350 rpm and homogenized at 2500-3500 rpm for 3-10 minutes; it is filtered and sterilized before filling, and the bacterial content is detected and quality control is carried out on toxins, immunogenicity, etc. to obtain the required post-sun repair essence.

[0070] The heating temperature in steps a-b can both be 75-80°C; the stirring speed in steps c-f can both be 200-400 rpm; the homogenization speed in steps c-f can both be 2000-4000 rpm.

[0071] Alfalfa (Medicago Sativa L.) is a perennial flowering plant of the legume family and is also known as lucerne. It contains nutrients such as protein, dietary fiber, minerals, and vitamins. In addition to being the world's oldest forage plant, it also has medicinal and ecological values. The active ingredients in alfalfa extract can inhibit the activity of 11β-hydroxysteroid dehydrogenase 1 (11β-HSD1), which can catalyze the conversion of inactive corticosterone into active cortisol. Inhibiting the activity of 11β-HSD1 enzyme can reduce the level of active cortisol in skin cells, thereby reducing the hardness of the fibroblast nuclear region and reducing skin aging and damage. Alfalfa extract is rich in galactomannan, which can enhance the skin barrier function, accelerate cell renewal, improve fiber quality and dermal density by increasing the expression of loricrin and aquaporin-3 (AQP3) and promoting the synthesis of extracellular matrix (ECM), thereby accelerating the repair of sun-exposed skin.

[0072] The fruit of Zanthoxylum armatum has a long tradition of use as an Ayurvedic medicine, and has traditionally been used for revitalization, anti - diabetes, antioxidant, anti - inflammatory, antibacterial and central nervous system (CNS) diseases, and can help reduce fatigue and relieve mental stress. Research shows that the fruit of Zanthoxylum armatum alleviates inflammation by regulating the expression level of toll - like receptor 4 (TLR - 4) and cytokine release, and by regulating the NF - kB and PPARγ pathways. In addition, the fruit of Zanthoxylum armatum can significantly increase the number of sensory neurons and neurite length, enhance the keratinocyte proliferation rate, and increase dopamine release. The proliferation of keratinocytes helps repair the skin barrier, thus preventing further damage to sunburned skin caused by environmental damage in daily life. As a neurotransmitter, dopamine can widely inhibit the expression of various inflammatory factors including TNF - α, MCP - 1, IL - 6 and IFN - γ in addition to regulating mood and improving cognitive function. In addition, sanshool in the fruit of Zanthoxylum armatum can reduce reactive oxygen species (ROS) induced by ultraviolet B (UVB), inhibit UVB - induced inflammation, relieve UV - exacerbated skin problems, thus playing an antioxidant and anti - aging role; induce autophagy and promote repair after tissue damage; inhibit MMP synthesis, which can reduce damage to the dermal matrix and help relieve wrinkles; and reduce photo - damage to the skin. The extract of the fruit of Zanthoxylum armatum exerts a synergistic effect in inhibiting inflammatory responses, promoting epidermal regeneration, accelerating autophagy, and enhancing mood, etc., to achieve the effect of repairing sunburned skin.

[0073] In this invention, each group of experiments is set with essence as an example, but it is not limited to the use of essence. This composition can also be applied to various skin care products such as lotions, creams, lyophilized powders, skin care lotions, etc. This essence is the above - mentioned key combination as well as emollients, thickeners, chelating agents and pH regulators. Preferably, the raw materials for preparing the essence for post - sun repair to enhance mood also include any one or at least two combinations of preservatives and fragrances.

[0074] Table 1 Proportion table of each component in Example 1

[0075]

[0076]

[0077] In an embodiment of the present invention, the preparation method of the above - mentioned essence includes the following steps:

[0078] a. Aqueous phase: Place water in a container with a stirring device, add the other parts of raw material combination A after fully wetting and mixing them into the water, stir fully until completely dissolved, and heat.

[0079] b. Oil phase; Mix and heat the raw material combination B, and stir until completely dissolved.

[0080] c. Emulsification: Add the mixture from step b to the aqueous phase from step a, and stir and homogenize for 5 minutes.

[0081] d. Add raw material C, stir and homogenize for 3 minutes. Stir to cool down to 45 °C.

[0082] e. Add the post-sun repair composition, stir and homogenize for 3 minutes.

[0083] f. Wait until the stirring cools down to 35 - 38 °C, add raw material combination D, stir and homogenize for 3 minutes, filter and sterilize before filling, and detect the bacterial content and conduct quality control on toxins, immunogenicity, etc. to obtain the required post-sun repair essence.

[0084] The heating temperature in steps a - b is 75 - 80 °C; the stirring speed in steps c - f is 20 - 40 rpm; the homogenization speed in steps c - f is 2000 - 4000 rpm.

[0085] Characterization data and effect data of the products in the examples and comparative examples

[0086] Examples 1 - 4 and Comparative Examples 1 - 3

[0087] The differences between Example 2, Example 3 and Example 1 are only the reduction of each active ingredient.

[0088] The difference between Example 4 and Example 1 is the increase in the content of individual raw material components.

[0089] The differences between Comparative Examples 1 - 3 and Example 2 are only the lack of 1 key composition component, and the difference between Comparative Example 3 and Example 2 is the non-addition of any composition components.

[0090] For Examples 2 - 3 and Comparative Examples 1 - 3, the specific ratios refer to Table 2, and the preparation method is the same as that of Example 1.

[0091] Table 2 Specific ratio table of each component of Examples 1 - 4 and Comparative Examples 1 - 3

[0092]

[0093] Table 3 Ratio table of Application Examples 1 - 6

[0094] Raw material INCI name Application Example 1 Application Example 2 Application Example 3 Application Example 4 Application Example 5 Application Example 6 Alfalfa extract 2 1 0.8 0 2 0 Pricklyash fruit extract 2 1 0.5 2 0 0 Water to 100 to 100 to 100 to 100 to 100 to 100

[0095] Efficacy evaluation test

[0096] 1. Rate of change of skin stratum corneum water content over time, transepidermal water loss (TWEL)

[0097] Test samples: Essence obtained from Examples 1-4 and Control Examples 1-3;

[0098] Test instruments: TWEL( Combo), Corneometer CM825

[0099] Test results:

[0100] (1) Demographic information of the subjects: A total of 36 subjects were included, all of whom were female. The average age was 32 years old, including 17 people aged 18-25, 7 people aged 26-30, 10 people aged 36-40, and 2 people aged 41-45.

[0101] (2) Statistics of adverse events during the test: None

[0102] (3) Results of skin physical and chemical data analysis

[0103] Table 4 Rate of change of stratum corneum water content of the subjects over time

[0104] Sample After 7 days of use After 14 days of use After 28 days of use Example 1 14.23％ 16.01％ 19.85％ Example 2 11.68％ 15.53％ 18.63％ Example 3 9.73％ 11.60％ 14.93％ Example 4 9.62％ 11.57％ 14.85％ Control Example 1 7.26％ 9.17％ 12.71％ Control Example 2 7.53％ 9.39％ 12.01％ Control Example 3 5.21％ 6.84％ 7.82％

[0105] As can be seen from Table 4, after using the essence, with the extension of the use time, the rate of change of the stratum corneum water content values of Examples 1-4 and Control Examples 1-3 increased significantly, indicating that the stratum corneum water content was improved and the skin barrier was repaired. After using the same time, the rate of change of the stratum corneum water content of Example 1 was the largest, indicating that the improvement effect of the stratum corneum water content was more significant after using the essence of Example 1.

[0106] There was no significant difference in the rate of change of the stratum corneum water content values between Example 4 and Example 3, indicating that when the content of the formula system changed within a certain range, it still had good stability and skin barrier repair ability, verifying the wide adaptability of the formula system.

[0107] From the comparison of the results of Example 2 and Control Examples 1-3, it can be seen that compared with not using one of hydrolyzed alfalfa extract and Zanthoxylum armatum fruit extract, simultaneously using hydrolyzed alfalfa extract and Zanthoxylum armatum fruit extract had the most significant improvement effect on the stratum corneum water content, indicating that the essence prepared from the composition had a stronger barrier repair effect.

[0108] Table 5 Rate of change of skin TWEL value of the subjects over time

[0109] Sample After 7 days of use After 14 days of use After 28 days of use Example 1 -15.77％ -18.25％ -24.62％ Example 2 -14.23％ -16.74％ -21.96％ Example 3 -12.78％ -14.65％ -17.21％ Example 4 -12.42％ -14.53％ -17.18％ Control Example 1 -9.53％ -10.71％ -12.16％ Control Example 2 -9.79％ -10.86％ -12.52％ Control Example 3 -2.15％ -3.57％ -4.10％

[0110] As can be seen from Table 5, the change rate of the TWEL value of the subjects' skin was the largest after using Example 1; compared with Control Examples 1-3, the change rate of the TWEL value of the subjects' skin was more significant after using Examples 1-4, indicating that the hydrolyzed alfalfa extract and the extract of Zanthoxylum armatum DC. fruits have a synergistic effect, and this composition has a stronger ability to reduce the TWEL value of the subjects' skin.

[0111] There was no significant difference in the change rate of the skin TWEL value between Example 4 and Example 3, indicating that when the content of the formula system changes within a certain range, it can still effectively repair the skin barrier function, reduce the transepidermal water loss rate, and the efficacy is not significantly affected by the excipient content.

[0112] After the subjects used Example 1, the TWEL value of the skin decreased significantly by 18.25% after 2 weeks; after using the product for 4 weeks, the TWEL decreased significantly by 24.62%. The above results suggest that under the test conditions of this time, Example 1 can reduce the skin TWEL value to the greatest extent and has the strongest repair effect.

[0113] 2. Determination of the content of TNF-α inflammatory factor in vitro - Testing with lipopolysaccharide-induced macrophages RAW264.7

[0114] Test model: Mouse mononuclear macrophage leukemia cell line RAW264.7

[0115] Test samples: The compositions obtained from Application Examples 1-6 at 0.02%

[0116] Positive control group: Dexamethasone at 100 μg / mL

[0117] Test method:

[0118] (1) Cytotoxicity test: Determine the most suitable sample concentration through the cytotoxicity test;

[0119] (2) Cell seeding: Dilute the cells with cell culture medium to the seeding density (the confluence reaches 45%-60% 24 hours after seeding), then seed them into a 96-well plate, 200 μL per well, and culture them in a CO2 incubator for 24 ± 2 hours;

[0120] (3) Drug administration: Discard the culture medium in the 96-well plate, and carry out the induction and drug administration operations. Add the culture medium containing a certain concentration of the test substance and LPS to the test substance wells, add the cell culture medium containing LPS to the negative control wells, add the culture medium containing the positive control and LPS to the positive control wells, and add the cell culture medium to the blank / solvent control wells, 200 μL per well. After drug administration, place the 96-well plate in a CO2 incubator and culture it for 24 h ± 2 h;

[0121] (4) Collection of cell supernatant: After the incubation culture is completed, collect the cell supernatant and place it in a 1.5 ml sterile centrifuge tube, and freeze it and store it in a -80°C refrigerator;

[0122] (5) Pre-experiment for ELISA detection: Since the expression level of TNF-α is relatively high after LPS induction, before the formal detection, the dilution ratio should be set to conduct a pre-experiment to explore and determine the dilution ratio of the experimental group for ELISA detection, so as to ensure that the ELISA detection values fall within the range of the standard curve.

[0123] (6) ELISA detection: Perform the detection according to the operation manual of the TNF-α ELISA detection kit.

[0124] Calculation formula for TNF-α inhibition rate: Inhibition rate (%) = (1 - T / C) × 100%;

[0125] In the formula, T is the average value of the TNF-α content of the test substance, and C is the average value of the TNF-α content of the negative control.

[0126] Table 6 Results table of type I TNF-α content

[0127]

[0128]

[0129] As can be seen from Table 6, the inhibition rate of the TNF-α content in the positive control is ≥ 25%, indicating that the test system is effective. The TNF-α content of the negative control under the action of LSP is up-regulated by a factor of ≥ 5 times compared with the blank control, and the stimulation conditions meet the test requirements. Compared with the negative control, the inhibition rate of the TNF-α content in Application Examples 1-5 has a significant difference, indicating that the sample has the ability to inhibit the TNF-α content, which can further illustrate that the sample has a soothing effect. Among them, Application Example 1 has the strongest inhibitory effect on TNF-α; compared with Application Examples 4-6, Application Examples 1-3 have a more significant inhibitory effect on TNF-α, indicating that the hydrolyzed alfalfa extract and the extract of Zanthoxylum armatum fruits have a synergistic effect, and this composition has a stronger ability to inhibit TNF-α.

[0130] 3. Product stability detection

[0131] Test sample: The sample obtained in Example 1

[0132] Test method: Internal control standard, observe the appearance color, viscosity, and pH within three months.

[0133] Table 7 Results table of product stability investigation

[0134] Time 45℃ 40℃ 37℃ 25℃ 5℃ -15℃ CYCLE Light 1W √ √ √ √ √ √ √ √ 2W √ √ √ √ √ √ √ √ 3W √ √ √ √ √ √ √ √ 4W √ √ √ √ √ √ √ √ 2M √ √ √ √ √ √ √ √ 3M √ √ √ √ √ √ √ √

[0135] As can be seen from Table 7 above, for the prepared essence liquid, within three months under high and low temperatures or light conditions, there are no significant changes in its color, viscosity, and pH, indicating that the essence liquid has good stability.

[0136] (1) Detection of the inhibitory effect on cortisol in Hacat cells (human immortalized keratinocytes)

[0137] Preparation of culture medium and solution: Add FBS 10% / double antibody 1% to DMEM culture medium; use 1× PBS buffer as diluent to prepare 1 mg / ml LPS solution;

[0138] (2) Cell seeding: 10,000 HaCat cells were seeded into a 96-well plate and cultured in a 37°C, 5% CO2 incubator for 24 h;

[0139] (3) Setting up a negative control group (NC), an agonist positive control group, an inhibitor positive control group (PC) and a sample group (Application Examples 1-6);

[0140] (4) Cortisol conversion reaction: dilute 50mM Corti sone solution to 100μM, add 50μl / well to the well plate, dilute and add samples to be tested: use complete culture medium as diluent, prepare different concentrations of sample working solution according to the sample test concentration table, add 50μl / well, a total of 100μl / well reaction system. Add an equal amount of complete culture medium to the blank control group, add 100μM Corti sone 100μl / well to the agonist positive control group, and add 200μM Metyrapone to the inhibitor positive control group. Place in a 37℃, 5% CO2 incubator for 24h and observe the cell morphology under a microscope.

[0141] (5) Take the supernatant from the well plate and detect the Cortisol content by ELISA. The specific steps are as follows:

[0142] ① Calculate the required enzyme label strips in advance, take out the kit 30 minutes before the experiment, and restore it to room temperature;

[0143] ② Gradient dilution of standard: dilute the standard with standard & sample diluent to 200, 100, 50, 12.5, 0 ng / mL;

[0144] ③ Collect the cell culture supernatant into a sterile centrifuge tube, centrifuge (4°C, 1000×g, 20min), and take the supernatant;

[0145] ④ Add 50 μl of standard working solution and test sample to each reaction well. Each group has 2 duplicate wells. Then add 50 μl of biotin-labeled cortisol antibody working solution to each reaction well, a total of 100 μl reaction system, and incubate at 37°C for 45 min.

[0146] ⑤ Discard the liquid and centrifuge to dry. Add 300 μl of washing solution to each reaction well, soak for 1 - 2 min and then centrifuge to dry. Repeat 4 times;

[0147] ⑥ Add 100 μl of HRP-labeled streptavidin working solution to each reaction well and incubate in an incubator at 37 °C for 30 min;

[0148] ⑦ Add 300 μL of washing solution to each reaction well, centrifuge to dry the washing solution after an interval of 30 s. Repeat 4 times;

[0149] ⑧ Add 90 μL of chromogenic agent to each reaction well and develop color at 37 °C in the dark for about 15 min;

[0150] ⑨ Add 50 μL of stop solution to each reaction well, immediately measure the OD value with an enzyme-linked immunosorbent assay (ELISA) reader at a wavelength of 450 nm. The OD value is inversely proportional to the cortisol content;

[0151] ⑩ According to the known concentration of the standard product and the measured OD value, fit a regression curve (R2 > 0.99) through ELISA Calc software. Substitute the OD value of the sample well into the ELISA Calc software for calculation, and calculate the X value from the Y value to obtain the actual cortisol concentration of the sample.

[0152] Table 8 Inhibition rate table of cortisol in Hacat cells (human immortalized keratinocytes)

[0153]

[0154]

[0155] As can be seen from Table 8, the inhibition rate of cortisol content in the inhibitor positive control group is ≥ 25%, indicating that the test system is effective. The cortisol content in the agonist positive control group under the action of cortisone is upregulated by a factor of ≥ 5 times compared with the blank control group, and the stimulation conditions meet the test requirements. Application Examples 1 - 5 all have obvious inhibitory effects on cortisol. Compared with Application Examples 2 - 5, Application Example 1 has the most obvious inhibitory effect on cortisol, indicating that the hydrolyzed alfalfa extract and the fruit extract of Zanthoxylum armatum have a synergistic effect, and this composition has a stronger ability to inhibit cortisol content.

[0156] In summary, the post-sun repair composition and application for enhancing mood, the cosmetics and their preparation methods proposed by the present invention, through the synergistic effect of the alfalfa extract and the fruit extract of Zanthoxylum armatum, can meet the requirements of the post-sun repair composition for moisturizing and soothing inflammation while reducing the cortisol level in skin cells and increasing the release of dopamine, so as to enhance mood and reduce the damage caused by negative emotions generated by post-sun skin appearance changes to the skin.

[0157] The present invention is different from enhancing mood through the sense of smell of aromatic scents. By applying the composition to the skin, the mood-enhancing effect is achieved by reducing the intracellular cortisol level and increasing dopamine release, completely avoiding the irritation of the olfactory sense by aromatic components and meeting the needs of odor-sensitive people for mood enhancement.

[0158] Through the synergistic effect of Medicago sativa extract and Zanthoxylum armatum DC. fruit extract, the present invention ensures that the efficacy of the composition can be stably exerted under various usage conditions. Even if some components are inactivated, the mood-enhancing efficacy of the composition can still be guaranteed, increasing the stability and ease of use of the composition, reducing the compatibility difficulty, and the combined use is significantly superior to the effect of using each component alone.

[0159] In one usage scenario of the present invention, the composition of the present invention can not only, through the synergistic effect of Medicago sativa extract and Zanthoxylum armatum DC. fruit extract, inhibit the inflammatory response through multi-target synergy, increase the expression of loricrin and aquaporin-3 (AQP3), promote the proliferation, differentiation of keratinocytes and the synthesis of extracellular matrix (ECM), and jointly achieve the effect of enhancing the skin barrier function and accelerating the repair of sunburned skin. In particular, Medicago sativa extract can reduce the level of cortisol in skin cells, and Zanthoxylum armatum DC. fruit extract can increase the release of dopamine. The two work synergistically to regulate neurotransmitters in multiple ways, and jointly achieve the effect of enhancing mood and preventing a series of negative impacts on the skin caused by neurotransmitters that produce negative emotions.

[0160] The technical features of the above-described embodiments can be combined arbitrarily. For the sake of brevity of description, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, it should be considered as the scope described in this specification.

[0161] The description and application of the present invention here are illustrative and do not intend to limit the scope of the present invention to the above embodiments. The effects or advantages involved in the embodiments may not be reflected in the embodiments due to various factors. The description of the effects or advantages is not used to limit the embodiments. The deformation and change of the embodiments disclosed here are possible, and the substitution and equivalent various components of the embodiments are well known to those of ordinary skill in the art. Those skilled in the art should clearly understand that the present invention can be implemented in other forms, structures, arrangements, proportions, and with other components, materials and parts without departing from the spirit or essential characteristics of the present invention. Other deformations and changes can be made to the embodiments disclosed here without departing from the scope and spirit of the present invention.

Claims

1. A mood-enhancing after-sun repair composition, characterized in that: The after-sun repair composition comprises: alfalfa extract and Zanthoxylum bungeanum fruit extract; the mass ratio of the alfalfa extract and the Zanthoxylum bungeanum fruit extract is (0.1-2%): (0.1～2％)。 2. The after-sun repair composition according to claim 1, characterized in that: The mass ratio of the alfalfa extract and the Zanthoxylum bungeanum fruit extract is (0.1-1%): (0.1-1%).

3. Use of the mood-enhancing after-sun repair composition according to any one of claims 1 to 2 in the preparation of cosmetics with after-sun repair efficacy.

4. A cosmetic, characterized in that: The cosmetic comprises the after-sun repair composition according to any one of claims 1 to 2.

5. The cosmetic according to claim 4, characterized in that: The cosmetics are essence, cream, lotion, freeze-dried powder or skin care water.

6. The cosmetic according to claim 5, characterized in that: The cosmetic is an essence; based on 100 parts by mass of the essence, it comprises 0.2 to 4 parts of an after-sun repair composition, 1.2 to 82 parts of a raw material combination A, 0.2 to 13 parts of a raw material combination B, 0.1 to 5 parts of a raw material combination C, and 0.11 to 6 parts of a raw material combination D; Wherein: the after-sun repair composition comprises 0.1-2 parts of alfalfa extract and 0.1-2 parts of Zanthoxylum bungeanum fruit extract; the raw material combination A comprises 0.1-25 parts of thickener A, 0.1-30 parts of skin conditioner A and 1-30 parts of moisturizer A; The raw material combination B includes 0.1 to 10 parts of an emulsifier and 0.1 to 5 parts of a skin conditioning agent B; The raw material combination C includes 0.1 to 5 parts of a pH regulator; The raw material combination D includes 0.1 to 5 parts of aromatics and 0.01 to 1 parts of preservatives; the above parts are all by mass.

7. The cosmetic according to claim 6, characterized in that: The raw material combination A also includes water, and the volume of the water is such that the weight parts of the essence reach 100 parts; The thickener A comprises at least one of acrylic acid (ester) / C10-30 alkyl acrylate cross-linked polymer, sodium acrylate / sodium acryloyldimethyl taurate copolymer, polyacrylamide, xanthan gum, cyclopentasiloxane, propylene glycol, cetearyl alcohol, carbomer, laureth-7, cetyl alcohol, stearyl alcohol and hydroxyethyl cellulose; The skin conditioning agent A and the skin conditioning agent B each independently include at least one of niacinamide, isohexadecane, olive oil, sodium hyaluronate, lauryl alcohol, avocado butter, allantoin, bisabolol, butylene glycol, squalane, panthenol, lactic acid, tocopherol and dipotassium glycyrrhizinate.

8. The cosmetic according to claim 6, characterized in that: The moisturizing agent A comprises at least one of hyaluronic acid, allantoin, glycerin, ceramide, sodium lactate, butylene glycol, hydrogenated grape seed oil, olive oil and shea butter; The emulsifier includes at least one of glyceryl stearate citrate, cetearyl glucoside (and) cetearyl alcohol, behenyl alcohol, behenyl trimethyl ammonium methyl sulfate, cocoglycerides and sorbitan olivate; The pH regulator includes at least one of arginine and lactic acid; the fragrance includes essence; and the preservative includes at least one of phenoxyethanol, ethylhexylglycerin and pentylene glycol.

9. A method for preparing the cosmetic according to any one of claims 6 to 8, characterized in that: The preparation method comprises: Step a, obtaining a water phase: placing water in a container with a stirring device, fully wetting and mixing the other parts of the raw material combination A except water, adding them into the water, stirring them fully until they are completely dissolved, and heating; Step b, obtaining an oil phase: mixing and heating the raw material combination B, and stirring until completely dissolved; Step c, emulsification step: adding the mixture of step b to the aqueous phase of step a, stirring and homogenizing for a set time; Step d, add raw material combination C, stir and homogenize for a set time; stir and cool to 40-45°C; Step e, adding the after-sun repair composition, stirring and homogenizing for a set time; Step f: After stirring and cooling to 35-38° C., add the raw material combination D, stir and homogenize for a set time to obtain the desired after-sun repair essence.

10. The preparation method according to claim 9, characterized in that: In step c, in the emulsification step, the mixture of step b is added to the aqueous phase of step a, and stirred and homogenized for 3 to 10 minutes; In step d, add raw material combination C, stir at a speed of 250-350 rpm for 3-5 minutes, and homogenize at a speed of 2500-3500 rpm for 3-10 minutes; stir and cool to 40-45° C.; Step e, adding the after-sun repair composition, stirring at a speed of 250-350 rpm for 3-5 minutes, and homogenizing at a speed of 2500-3500 rpm for 3-10 minutes; Step f, after stirring and cooling to 35-38° C., add the raw material combination D, stir at 250-350 rpm and homogenize at 2500-3500 rpm for 3-10 minutes; filter and sterilize before filling, detect the bacterial content and perform quality control on toxins, immunogenicity, etc. to obtain the desired after-sun repair essence.

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