Skin barrier repairing composition and application thereof

Abstract

The invention discloses a skin barrier repairing composition and application thereof, and belongs to the technical field of cosmetics. The repairing composition is prepared from rosa multiflora flower extract, rose flower extract, lactobacillus / soybean milk fermentation product filtrate, vitamin E, vitamin B5 and sodium hyaluronate. Compared with the prior art, the repair composition provided by the invention is compounded by selecting specific types of substances, has a good synergistic effect among the components, can effectively activate a PPAR gamma / RXR pathway, has a good inhibition effect on free radicals, and can obviously promote cell healing. Therefore, the repair composition provided by the invention can be widely applied to preparation of skin care products, and the obtained skin care products are mild and stable and have excellent effects of moisturizing, whitening, tightening and removing wrinkles, improving skin barrier and relieving skin redness.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and particularly relates to a skin barrier repair composition and its application. Background Art

[0002] In the modern environment, factors such as ultraviolet radiation, air pollution, chemical irritants, and bad living habits have led to an increasingly prominent problem of damaged skin barrier, manifested as dryness, sensitive flushing, inflammation, and oxidative stress. According to clinical statistics, more than 60% of adults have varying degrees of skin barrier dysfunction, and there is an urgent need for safe and effective repair means. Summary of the Invention

[0003] The purpose of the present invention is to overcome the deficiencies of the above-mentioned prior art and provide a skin barrier repair composition and its application that can achieve good repair effects of the repair composition through multiple pathways such as activating the PPARγ / RXR pathway, inhibiting the activity of free radicals, and promoting cell healing, and macroscopically has excellent effects of moisturizing, whitening, firming and removing wrinkles, enhancing the skin barrier, and relieving skin flushing.

[0004] To achieve the above purpose, in the first aspect of the present invention, the present invention provides a skin barrier repair composition, and the repair composition includes Rosa centifolia flower extract, Rosa rugosa flower extract, Lactobacillus / Soybean Ferment Filtrate, vitamin E, vitamin B5, and sodium hyaluronate.

[0005] The repair composition provided by the present invention selects specific types of substances for compounding, and there is a good synergistic effect between the components, which can effectively activate the PPARγ / RXR pathway, and has a good inhibitory effect on free radicals, promotes the expression of SPRR1, strengthens the mechanical barrier of the stratum corneum, and at the same time can significantly increase the cell healing rate; thus, it has excellent effects of moisturizing, whitening, firming and removing wrinkles, enhancing the skin barrier, and relieving skin flushing macroscopically.

[0006] Specifically, the extract of Rosa centifolia is rich in substances such as vitamin C, anthocyanins, vitamin E, and flavonoids, which have the effects of whitening and lightening spots, reducing fine lines, moisturizing and nourishing, soothing the skin, as well as antioxidant and anti-inflammatory effects. The main active ingredients of the rose extract include volatile oils, flavonoids, polyphenols, etc., which have the effects of brightening the skin tone, fading age spots, and antioxidant. The active ingredients in the filtrate of Lactobacillus / Soybean Fermentation Product mainly include organic acids, polypeptides, exopolysaccharides, soybean isoflavone transformation products, antimicrobial peptides, and short-chain fatty acids, which have the effects of relieving skin sensitivity, moisturizing and repairing, and whitening. Vitamin E has certain antioxidant, moisturizing and skin-nourishing, age spot fading, and repair effects. Vitamin B5 also has certain effects of moisturizing and nourishing, antioxidant, soothing and repairing, and brightening the skin tone. Sodium hyaluronate has multiple effects, not only can lock water and moisturize, but also has certain auxiliary repair and soothing effects. Based on the above-mentioned submitted effects of the components in the present invention, there is also a certain interaction between the components. When the components in the present invention are selected for combination, the repair effect and antioxidant effect of the obtained repair composition are more excellent; when applied to subsequent skin care products, the comprehensive effect of the obtained skin care products is also better.

[0007] As a preferred embodiment of the repair composition of the present invention, the repair composition comprises the following components in parts by mass: 5-15 parts of Rosa centifolia extract, 5-15 parts of rose extract, 0.5-3.5 parts of filtrate of Lactobacillus / Soybean Fermentation Product, 0.0001-0.0003 parts of vitamin E, 0.1-0.4 parts of vitamin B5, and 20-40 parts of sodium hyaluronate.

[0008] As a preferred embodiment of the repair composition of the present invention, the repair composition comprises the following components in parts by mass: 8-12 parts of Rosa centifolia extract, 8-12 parts of rose extract, 1.5-2.5 parts of filtrate of Lactobacillus / Soybean Fermentation Product, 0.00012-0.0002 parts of vitamin E, 0.15-0.3 parts of vitamin B5, and 25-35 parts of sodium hyaluronate.

[0009] The present invention has found through research that the parts by mass of the components in the repair composition will affect the comprehensive performance of the product. When the parts by mass of the components are further selected within the above preferred ranges, the ability of the obtained product to promote cell healing and antioxidant ability are better; when applied to subsequent skin care products, the obtained skin care products can quickly and long-lastingly achieve good moisturizing and whitening, improve the skin barrier ability, relieve skin sensitivity and flushing, and firm and lift the skin.

[0010] As a preferred embodiment of the repair composition of the present invention, based on the total mass of the repair composition, the mass percentage of Rosa centifolia extract and rose extract is 36-40%.

[0011] The present invention has found that when the sum of the mass percentages of the Rosa centifolia flower extract and the rose flower extract in the repair composition is further selected within the above range, the interaction between the components can be better exerted, and the comprehensive effect of the obtained product is better.

[0012] As a preferred embodiment of the repair composition of the present invention, the mass ratio of the Rosa centifolia flower extract to the rose flower extract is 1:(0.8 - 1.2).

[0013] The present invention has found that the mass ratio of the Rosa centifolia flower extract to the rose flower extract also affects the comprehensive performance of the product. When the mass ratio of the Rosa centifolia flower extract to the rose flower extract is further selected within the above range, the comprehensive performance of the obtained product is better.

[0014] As a preferred embodiment of the repair composition of the present invention, the weight-average molecular weight of the sodium hyaluronate is 1,000,000 Da - 1,600,000 Da.

[0015] In the second aspect of the present invention, the present invention provides the use of the repair composition in the preparation of skin care products.

[0016] The repair composition provided by the present invention has good effects of promoting cell healing and antioxidant, therefore, it can be widely used as an active composition in the preparation of skin care products.

[0017] As a preferred embodiment of the use of the present invention, the skin care products include skin care lotion, milk, essence, cream, mask.

[0018] In the third aspect of the present invention, the present invention provides a rose essential oil, and the rose essential oil includes the repair composition of the present invention.

[0019] As a preferred embodiment of the rose essential oil of the present invention, based on the mass of the rose essential oil, the mass percentage of the repair composition is 0.1 - 5%.

[0020] As a preferred embodiment of the rose essential oil of the present invention, the rose essential oil further includes squalane and deionized water.

[0021] As a preferred embodiment of the rose essential oil of the present invention, the rose essential oil includes the following components in mass percentages: 0.1 - 5% repair composition, 15 - 25% squalane, and the balance deionized water.

[0022] By adding an appropriate mass percentage of the repair composition of the present invention to the rose essential oil provided by the present invention, it not only has the characteristics of stable properties, but also has excellent comprehensive effects, which are reflected in having good moisturizing and whitening effects, improving skin barrier ability, improving skin sensitivity and flushing, and removing wrinkles and firming the skin.

[0023] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0024] The repair composition provided by the present invention is prepared by compounding specific types of substances, and there is a good synergistic effect among the components, which can effectively activate the PPARγ / RXR pathway, has a good inhibitory effect on free radicals, and can significantly promote cell healing. Therefore, the repair composition provided by the present invention can be widely applied to the preparation of skin care products, and the obtained skin care products are mild and stable and have excellent effects of moisturizing, whitening, firming and removing wrinkles, enhancing the skin barrier and relieving skin flushing. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is the experimental result diagram of the negative control group in the cell healing rate test of Effect Example 1;

[0026] Figure 2 It is the experimental result diagram of the positive control group in the cell healing rate test of Effect Example 1;

[0027] Figure 3 It is the experimental result diagram of the repair composition prepared in Example 1 in the cell healing rate test of Effect Example 1;

[0028] Figure 4 It is the experimental result diagram of the repair composition prepared in Example 3 in the cell healing rate test of Effect Example 1;

[0029] Figure 5 It is the experimental result diagram of the repair composition prepared in Example 4 in the cell healing rate test of Effect Example 1;

[0030] Figure 6 It is the experimental result diagram of the repair composition prepared in Example 5 in the cell healing rate test of Effect Example 1;

[0031] Figure 7 It is the experimental result diagram of the repair composition prepared in Comparative Example 10 in the cell healing rate test of Effect Example 1. DETAILED DESCRIPTION OF THE INVENTION

[0032] To better illustrate the purpose, technical solution and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0033] The reagents, methods and equipment used in the present invention are all conventional reagents, methods and equipment in the art unless otherwise specified; and unless otherwise specified, the raw materials used in parallel experiments are of the same batch.

[0034] Rosa centifolia flower extract: Centifolia Rose Essence, purchased from Ashland (China) Investment Co., Ltd.;

[0035] Rose extract: Dark red rose essence, purchased from Beijing Weiming Shiguang Biotechnology Co., Ltd.;

[0036] Lactobacillus / soy milk fermentation product filtrate: purchased from Xianting (Guangzhou) Trading Co., Ltd.;

[0037] Vitamin E: purchased from DSM Vitamins (Shanghai) Co., Ltd.

[0038] Vitamin B5: purchased from DSM Vitamins (Shanghai) Co., Ltd.;

[0039] Sodium hyaluronate: purchased from Shandong Galaxy Biotechnology Co., Ltd., with a weight average molecular weight of 1.5 million Da.

[0040] Examples 1-6 and Comparative Examples 1-4

[0041] Examples 1-6 of the present invention and Comparative Examples 1-4 provide a repair composition, the components (parts by mass) of the repair composition are shown in Table 1;

[0042] Table 1

[0043]

[0044]

[0045] The preparation method of the repair composition provided in Example 1 is: mixing the components evenly to obtain the repair composition.

[0046] The preparation methods of the repair compositions provided in Examples 2-6 and Comparative Examples 1-4 are consistent with that in Example 1.

[0047] Comparative Example 5

[0048] The comparative example of the present invention provides a repair composition, the only difference between the repair composition and Example 1 is that Damascus rose essence (purchased from Yin Gu Aromatic Technology Co., Ltd.) is used instead of Rosa centifolia flower extract.

[0049] Comparative Example 6

[0050] The comparative example of the present invention provides a repairing composition, the only difference between the repairing composition and Example 1 is that the lactobacillus / soy milk fermentation product filtrate is replaced by citrus fruit extract (purchased from Shanghai Jiakai Biological Co., Ltd.).

[0051] Comparative Example 7

[0052] The comparative example of the present invention provides a repairing composition, wherein the only difference between the repairing composition and Example 1 is that vitamin C is used instead of vitamin E.

[0053] Comparative Example 8

[0054] The comparative example of the present invention provides a repair composition, and the only difference between the repair composition and that of Example 1 is that acetyl heptapeptide-4 is used to replace vitamin B5.

[0055] Comparative Example 9

[0056] The comparative example of the present invention provides a repair composition, and the only difference between the repair composition and that of Example 1 is that a mixture of asiaticoside and ectoine with a mass ratio of 1:1000 is used to replace vitamin B5.

[0057] Comparative Example 10

[0058] The comparative example of the present invention provides a repair composition, and the only difference between the repair composition and that of Example 1 is that ectoine is used to replace sodium hyaluronate.

[0059] Application Examples 1-7, Comparative Application Examples 1-10 and Blank Application Example

[0060] The application examples, comparative application examples and blank application example of the present invention provide a rose essential oil, and the components (mass percentages) of the rose essential oil are shown in Table 2; among them, the repair compositions in Application Examples 1-6 are respectively the repair compositions prepared in Examples 1-6, and the repair compositions in Comparative Application Examples 1-10 are respectively the repair compositions prepared in Comparative Examples 1-10; for example, the repair composition used in Application Example 6 is the repair composition prepared in Example 6, and so on; the repair composition used in Application Example 7 is the repair composition prepared in Example 1;

[0061] Table 2

[0062]

[0063] The preparation method of the rose essential oil provided in Application Example 1 is as follows:

[0064] Weigh deionized water, add the repair composition and stir until it is completely dispersed; then add squalane and stir evenly to obtain the rose essential oil.

[0065] The preparation methods of the rose essential oils provided in Application Examples 2-7, Comparative Application Examples 1-10 and Blank Application Example are the same as that of Application Example 1, and if there are no relevant components, they will not be added.

[0066] Effect Example 1

[0067] The effect example of the present invention verifies the in vitro antioxidant performance, in vitro cell healing ability and the influence on RXRB of the repair compositions prepared in the examples and comparative examples, including the following aspects:

[0068] 1. PTIO free radical scavenging experiment

[0069] Free radicals are a class of highly reactive substances. When the free radical metabolism in the body is in an unbalanced state, excessive free radicals will cause oxidative damage to the body (including skin tissues), reduce the collagen content and the strength of elastic fibers, and lead to skin relaxation and wrinkles. The solution of PTIO free radicals is purple. When the wavelength is 560 nm, the PTIO free radical solution has a strong absorption. In the reaction system, after the sample reacts with the PTIO free radicals, the solution fades and the absorbance value decreases. By measuring the change in the absorbance of PTIO at a wavelength of 560 nm, the scavenging effect of the sample on PTIO free radicals is evaluated.

[0070] This test is carried out with reference to the "In Vitro Efficacy Evaluation Method for Cosmetics / Cosmetic Raw Materials - PTIO Free Radical Scavenging Experiment" (CTC-SOP-031-2024) of China National Institute for Food and Drug Control (Beijing) Cosmetics Technology Co., Ltd.; specifically, it includes the following steps:

[0071] (1) Main instruments

[0072] Fluorescence / Chemiluminescence Multifunctional Microplate Monitoring System: Thermo Scientific, Varioskan fisherLUX N / M, USA.

[0073] (2) Main reagents

[0074] PBS: Procell, WHB824N011;

[0075] PTIO: TCI, 362TB-MQ;

[0076] Absolute ethanol: Sinopharm, 20160401;

[0077] VC: SIGMA, SLCC2424.

[0078] (3) Test samples and control samples

[0079] Test sample: The repair composition prepared in the examples and comparative examples;

[0080] Negative control: PBS solution;

[0081] Positive control: PBS solution containing 100 μg / mL vitamin C.

[0082] (4) Test method

[0083] a. Preparation of reaction solution: Prepare a 150 μg / mL PTIO solution using a PBS solution containing 1% ethanol, and stir with a glass rod until dissolved to obtain the PTIO reaction solution;

[0084] b. Test grouping: The test sets up a sample group, a sample blank, a negative control and a negative blank, a positive control and a positive blank;

[0085] c. Preparation of reaction system: In the sample group, negative control and positive control, add PTIO reaction solution and corresponding samples. In the sample blank, negative blank and positive blank, add PBS solution containing 1% ethanol and corresponding samples;

[0086] d. Detection by microplate reader: After the reaction system is mixed evenly, incubate at room temperature for 120 min. After the reaction ends, detect the change in the content of PTIO free radicals under the condition of a wavelength of 560 nm; calculate the PTIO free radical scavenging rate and record it in Table 3. At the same time, use SPSS software for analysis. The PTIO free radical scavenging rate of the positive control is higher than that of the negative control and has statistical significance (p < 0.05), indicating that the test conditions are effective in this experiment; the PTIO free radical scavenging rate of the sample test group is higher than that of the negative control and has statistical significance (p < 0.05), indicating that the sample has the ability to scavenge PTIO free radicals at this concentration, indicating that the sample has antioxidant effects;

[0087] Among them, the PTIO free radical scavenging rate = (1 - (OD560 value of the sample group or positive control - OD560 value of the blank of the sample group or positive control) / (OD560 value of the negative control - OD560 value of the negative blank)) * 100%; the OD560 value is the absorbance value of the test sample at 560 nm.

[0088] 2. In vitro cell healing ability

[0089] The epidermis is the most important part for skin repair. Keratinocytes are the main constituent cells of the epidermis, and the proliferation of keratinocytes plays a key role in skin repair. Therefore, by detecting the healing rate of human immortalized keratinocytes (HaCaT) after the action of the sample, the repair efficacy of the test sample is evaluated.

[0090] This test is carried out in accordance with the "In Vitro Efficacy Evaluation Method for Cosmetics / Cosmetic Raw Materials - Cell Healing Ability Test" (CTC-SOP-036-2024) of China National Institute for Food and Drug Control (Beijing) Cosmetics Technology Co., Ltd.; the specific steps are as follows:

[0091] (1) Main instruments

[0092] Carbon dioxide incubator: Thermo, 371, USA;

[0093] Biological safety cabinet: Esco, Esco AC2-4S1, Singapore;

[0094] Inverted microscope: ZEISS, Axio Vert.A1, Germany;

[0095] Fluorescence / Chemiluminescence Multifunctional Microplate Monitoring System: Thermo Scientific, Varioskan fisherLUX N / M, USA.

[0096] (2) Main reagents, consumables, cells

[0097] PBS: Procell, WHB824N011;

[0098] DMEM medium: Gibco, 6123134;

[0099] Fetal bovine serum: Sijiqing, 22100705;

[0100] CCK-8 kit: Beyotime, 022124240625;

[0101] HaCaT cells: Peking Union Medical College Cell Bank, 1101HUM-PUMC000373.

[0102] (3) Test samples and control samples

[0103] Test samples: The repair compositions prepared in the examples and comparative examples were diluted with DMEM medium to a concentration of 0.2% and then filtered and sterilized for standby (this concentration was the appropriate concentration determined after cytotoxicity testing);

[0104] Negative control: DMEM medium;

[0105] Positive control: DMEM medium containing 1% fetal bovine serum.

[0106] (4) Test methods

[0107] a. Cell seeding: HaCaT cells were seeded into a 6-well plate at an inoculation density of 5×10 5 cells / well and incubated in an incubator (37°C, 5% CO2) for 24 h;

[0108] b. Cell administration: When the cell confluence rate in the 6-well plate reached 80% - 90%, a cell scratch was made perpendicular to the 6-well plate with a P200 pipette tip. The medium containing a certain concentration of the test sample was added to the test sample wells, the cell medium was added to the negative control wells, and the medium containing 1% fetal bovine serum was added to the positive control wells. 1 mL of sample was added to each well, and 3 replicates were set for each group. The cell scratch pictures at 0 h after drug addition were taken with an inverted microscope. After drug administration, the 6-well plate was placed in an incubator (37°C, 5% CO2) and continued to be cultured for 24 h;

[0109] c. Cell scratch photography: After the culture was completed, the cell scratch pictures at 24 hours after adding the drug were taken with an inverted microscope; the cell healing rate was calculated and recorded in Table 3; at the same time, SPSS software was used for analysis. Compared with the negative control, the HaCaT cell healing rate increased in the positive control and there was a statistically significant difference (p < 0.05), indicating that the test conditions were effective in this experiment; compared with the negative control, the HaCaT cell healing rate increased in the sample test group and there was a statistically significant difference (p < 0.05), indicating that the sample had the ability to promote the healing of HaCaT cells at this concentration, suggesting that the sample had a repair effect;

[0110] Among them, the cell healing rate = (1 - (scratch area at 24h / scratch area at 0h)) * 100%.

[0111] 3. Influence on RXRB

[0112] RXRB has multiple functions in the body. For example, on the one hand, it can regulate gene transcription and cell differentiation. As a nuclear receptor, RXRB can form heterodimers with retinoic acid receptors (RAR) or itself, bind to specific DNA sequences (such as retinoic acid response element RARE), and regulate the expression of downstream genes. For example, in the retinoic acid (such as retinoic acid, retinol) signaling pathway, RXRB acts synergistically with RAR to activate genes related to cell differentiation and proliferation, promote the orderly differentiation of epidermal keratinocytes, reduce the accumulation of senescent cells, and thus maintain the integrity and function of the skin structure. On the other hand, it can participate in antioxidant and anti-inflammatory responses. RXRB reduces the accumulation of ultraviolet-induced reactive oxygen species (ROS) by regulating the expression of antioxidant enzymes (such as superoxide dismutase SOD, glutathione peroxidase), thereby reducing the damage of oxidative stress to skin collagen and DNA. In addition, its signaling pathway can also inhibit the release of pro-inflammatory factors (such as IL-6, TNF-α) and relieve the damage of chronic inflammation to the skin barrier. On the third hand, it can regulate lipid metabolism. RXRB is the core co-activator of the PPAR pathway. The PPAR family (α, β / δ, γ) needs to form heterodimers with the RXR family (including RXRA, RXRB, RXRG) to bind to the peroxisome proliferator response element (PPRE) in the promoter region of the target gene and activate downstream gene transcription.

[0113] (1) Main instruments

[0114] Carbon dioxide incubator: Thermo, 371, USA.

[0115] (2) Main reagents, consumables, cells

[0116] PBS: Procell, WHB824N011;

[0117] DMEM medium: Gibco, 6123134;

[0118] HaCaT cells: Peking Union Medical College Cell Bank, 1101HUM-PUMC000373.

[0119] (3) Test samples and control samples

[0120] Test samples: The repair compositions prepared in the examples and comparative examples were diluted with DMEM medium to a concentration of 0.1% and then filtered and sterilized for later use (this concentration was the appropriate concentration determined after cytotoxicity testing);

[0121] Blank control: DMEM medium.

[0122] (4) Test methods

[0123] a. Cell seeding: HaCaT cells were seeded into a 6-well plate at an inoculation density of 3×10 5 cells / well and incubated in an incubator (37 °C, 5% CO2) for 24 h;

[0124] b. Cell administration: After incubation for 24 h, the DMEM medium was aspirated, and after carefully rinsing once with PBS, the cells were treated with the DMEM medium containing the sample for 24 h;

[0125] c. RNA extraction: The cell supernatant was discarded, and the cells were lysed with Trozol to extract RNA;

[0126] d. Library construction and sequencing: Rigorous quality control is performed on the RNA samples, mainly through the Agilent 2100 bioanalyzer to accurately detect the RNA integrity. Oligo dT magnetic beads are used to enrich mRNA from total RNA. After fragmentation, random hexamer primers are used to synthesize the first-strand cDNA, followed by the synthesis of the second-strand cDNA. After end repair, A-tailing, adapter ligation, fragment selection, amplification, and purification, the library is ready. After the library construction is completed, it is first preliminarily quantified using the Qubit 2.0 Fluorometer, and the library is diluted to 1.5 ng / μl. Subsequently, the Agilent 2100 bioanalyzer is used to detect the insert size of the library. After the insert size meets the expectation, qRT-PCR is used to accurately quantify the effective concentration of the library (the effective concentration of the library is higher than 1.5 nM) to ensure the library quality. After passing the library inspection, different libraries are pooled according to the requirements of the effective concentration and the target output data volume and then subjected to Illumina sequencing. The basic principle of sequencing is Sequencing by Synthesis. Four fluorescently labeled dNTPs, DNA polymerase, and adapter primers are added to the flow cell for sequencing. When extending the complementary strand in each sequencing cluster, each added fluorescently labeled dNTP can release the corresponding fluorescence. The sequencer captures the fluorescence signal and converts the optical signal into a sequencing peak through computer software, thereby obtaining the sequence information of the fragment to be tested;

[0127] e. Differential gene analysis: The HISAT2 software is used to quickly and accurately align the Clean Reads with the reference genome to obtain the mapping information of the Reads on the reference genome. According to the position information of the gene alignment on the reference genome, the number of reads covering each gene (including newly predicted genes) from the start to the end range is counted. Reads with a mapping quality value lower than 10, non-uniquely mapped reads, and reads mapped to multiple regions of the genome are filtered out respectively. This part of the analysis uses the featureCounts tool in the subread software. Quantitative analysis of gene expression levels is performed on each sample separately, and then the expression matrix of all samples is combined. After the gene expression quantification is completed, statistical analysis needs to be performed on its expression data to screen for genes with significantly different expression levels in different states of the samples. The differential analysis is mainly divided into three steps: 1) First, the original readcount is normalized, mainly for the correction of the sequencing depth; 2) Then, the probability of the hypothesis test (pvalue) is calculated using the statistical model; 3) Finally, multiple hypothesis test correction is performed to obtain the FDR value; Finally, the results of calculating the upregulation rate of RXRB are shown in Table 3;

[0128] Among them, the up-regulation rate of RXRB = (gene expression level of the sample - gene expression level of the blank control) / gene expression level of the blank control.

[0129] Table 3

[0130]

[0131] Among them, in Table 3, "*" indicates p < 0.05 compared with the negative control group; "#" indicates p < 0.05 compared with the negative control group between the positive control group and the negative control group.

[0132] It can be seen from Table 3 that when the technical solution provided by the present invention is adopted, the obtained repair composition has excellent antioxidant effect and cell healing promoting effect; specifically, the scavenging rate of the obtained repair composition for PTIO free radicals is above 80.66%, the cell healing rate is above 15.81%, and the obtained composition can significantly up-regulate RXRB, and the up-regulation rate is above 2190%;

[0133] It can be seen from Examples 1-6 that the mass parts of the components, the mass ratio between two certain components, and the sum of the mass percentages of two certain components also affect the comprehensive effect of the repair composition;

[0134] It can be seen from Example 1 and Comparative Examples 1-4 that the components provided by the present invention are indispensable. When either one of the components is not added or the mass parts of other components are used to make up, the scavenging ability of the obtained product for PTIO free radicals and the cell healing rate both decrease significantly; it can be seen from Example 1 and Comparative Examples 5-10 that when other similar components are used to replace the components in the present invention or further components are added, the effects of the present invention cannot be achieved.

[0135] In addition, in the cell healing rate test, the states of the cell healing rates at 0 h and 24 h of the negative control group, the positive control group, Example 1, Examples 3-5, and Comparative Example 10 are respectively as Figures 1-7 shown, and it can also be intuitively seen from Figures 1-7 that the repair composition provided by the present invention has excellent cell healing promoting effect.

[0136] Effect Example 2

[0137] The present invention verifies the irritation of the rose essential oil prepared in the application example, the comparative application example, and the blank application example; specifically, it is tested by the human skin patch test:

[0138] Recruit 30 volunteers, 15 males and 15 females, aged 20 - 50 years old. Using the closed patch test method, place an equal amount (0.020 mL) of the test sample (the prepared rose essential oil) in a specific patch tester, apply it to the volunteers' arms with a low-allergenic tape, gently press it to make it evenly adhere to the skin, and leave it for 24 h; the blank control group is distilled water. After 24 h, remove the patch tester, observe the skin reaction at 0.5 h, 24 h, and 48 h, and record the results. The adverse reaction grades of the skin are shown in Table 4 below;

[0139] Table 4

[0140]

[0141] The results show that the rose essential oils prepared in the application examples, comparative application examples, and blank application examples are all negative reactions after human patch testing, and they are non-irritating to the human skin and safe.

[0142] Effect Example 3

[0143] The effect examples of the present invention conduct human efficacy verification on the rose essential oils prepared in Application Examples 1 - 7, Comparative Application Examples 1 - 10, and Blank Application Example, including the following steps:

[0144] 1. Test items

[0145] Skin rapid optical imaging test (AEVA-HE), facial image acquisition (VISIA), skin moisture content test (Corneometer), skin moisture loss test (Vapometer), skin patch sampling, skin component analysis (Raman), and facial image analysis (Image-Pro plus), skin glossiness test (Glossymeter), skin heme test (Mexameter), skin multiphoton tomography test (MPT).

[0146] 2. Test areas

[0147] Skin rapid optical imaging test (AEVA-HE): Fine lines on a randomly selected cheek;

[0148] Facial image acquisition (VISIA): The entire face;

[0149] Skin moisture content test (Corneometer), skin moisture loss test (Vapometer), skin patch sampling, skin component analysis (Raman), and facial image analysis (Image-Pro plus): The left cheek;

[0150] Skin glossiness test (Glossymeter), skin heme test (Mexameter), skin multiphoton tomography test (MPT): Right cheek.

[0151] 3. Testing instruments

[0152] Skin moisture content tester (Cornemeter, CM825, Courage and Khazaka, Germany);

[0153] Skin moisture loss tester (Vapometer, SWL5201, Delfin, Finland);

[0154] Skin glossiness tester (Glossymeter, GL200, Courage and Khazaka, Germany);

[0155] Skin heme tester (Mexameter, Mx18, Courage and Khazaka, Germany);

[0156] Skin composition analysis system (Raman, River DInternational B.V., Gen2, Netherlands);

[0157] Skin multiphoton tomography system (Multiphoton Tomography, MPT flex10, Jenlab, Germany);

[0158] Skin rapid optical imaging system (AEVAHE, Breuckmann, France);

[0159] Facial image acquisition system (VISIACR, Canfield, USA).

[0160] 4. Testing time points

[0161] Initial value, 2 weeks and 4 weeks after product use.

[0162] 5. Subject requirements

[0163] 1) Inclusion criteria

[0164] (1) Female subjects aged 26 - 33 years old;

[0165] (2) Facial skin sensitivity (screened by 10% lactic acid);

[0166] (3) Those who self-report that their perceived facial age is consistent with their physiological age;

[0167] (4) Those who have never used essence oil in daily life;

[0168] (5) Subjects are ordinary people in daily life, without special working or living environments (such as exposure to the sun, night work, etc.) and without specific intervention measures; the subjects are in good health during the test.

[0169] (6) Voluntarily participate and sign the informed consent form.

[0170] (7) Those who can complete the specified content according to the requirements of the test protocol.

[0171] 2) Exclusion criteria

[0172] (1) Women who are planning to be pregnant, pregnant, lactating, or within 6 months after childbirth.

[0173] (2) Those suffering from severe systemic diseases, immunodeficiency, or autoimmune diseases.

[0174] (3) Those suffering from allergic diseases or having had cosmetic allergies in the last 1 - 2 years.

[0175] (4) Those with dermatological diseases in the test area or who are receiving drug treatment.

[0176] (5) Participants who have a cold, flu, or other acute diseases at the start of the test, which may affect the study.

[0177] (6) Those who have participated in other tests on the test site now or in the last month.

[0178] (7) Those considered unsuitable for participation by dermatologists.

[0179] 3) Grouping information

[0180] There are 6 volunteers in each group, and a total of 108 volunteers.

[0181] 6. Test method

[0182] Based on a full - face experimental design, 108 eligible female subjects participated in the test. During the test, the subjects used the distributed test products (products prepared from the application example, comparative application example, and blank application example) on the full face, once in the morning and once in the evening every day for 4 consecutive weeks. Throughout the test period, the test products were used to replace the subjects' original essence products, and the original cleansing products, toner, lotion, sunscreen products, and makeup were continued to be used, and the product brands and usage methods were kept unchanged. Before using the test products, and 2 weeks and 4 weeks after using the test products, rapid skin optical imaging tests were conducted on the fine lines of one randomly selected cheek; skin moisture content tests, skin moisture loss tests, skin patch sampling, and skin component analysis were conducted on the left cheek; skin glossiness tests, skin heme tests, and skin multiphoton tomography scans were conducted on the right cheek; facial image acquisition and analysis were conducted on the full face.

[0183] 7. Test environment

[0184] Constant temperature and humidity environment, temperature is 21±1°C, humidity is 50±10%RH.

[0185] 8. Test results

[0186] By using the corresponding instrument to test the corresponding parameters, calculate the change rate of the corresponding parameters after 2 weeks and 4 weeks of use compared with the initial value, and the obtained results are shown in Table 5-6;

[0187] Table 5

[0188]

[0189]

[0190]

[0191] Table 6

[0192]

[0193]

[0194]

[0195]

[0196]

[0197] As can be seen from Table 5-6, when the technical solution provided by the present invention is adopted, the obtained rose essential oil has excellent immediate and long-term human body effects; specifically, the absolute values of the change rates of Rz and Ra shown in the rapid skin optical imaging at 2 weeks are above 3.19% and above 4.41% respectively, and the absolute values of the change rates of Rz and Ra shown in the rapid skin optical imaging at 4 weeks are above 3.91% and above 4.99% respectively; the change rates of skin moisture content at 2 weeks and 4 weeks are above 12.91% and above 17.31% respectively; the absolute values of the change rates of skin moisture loss at 2 weeks and 4 weeks are above 10.41% and above 11.97% respectively; the change rates of Gloss value and Gloss with DSC shown in skin glossiness at 2 weeks are above 10.76% and above 13.81% respectively, and the change rates of Gloss value and Gloss with DSC shown in skin glossiness at 4 weeks are above 15.27% and above 17.47% respectively; the absolute values of the change rates of skin heme at 2 weeks and 4 weeks are above 2.91% and above 7.41% respectively; the change rates of stratum corneum moisture content and ceramide content shown in skin component analysis at 2 weeks are above 19.36% and above 6.27% respectively, and the change rates of stratum corneum moisture content and ceramide content shown in skin component analysis at 4 weeks are above 21.77% and above 12.31% respectively; the absolute values of the change rates of the proportion of red area and glossiness shown in facial image analysis at 2 weeks are above 4.44% and above 17.28% respectively, and the absolute values of the change rates of the proportion of red area and glossiness shown in facial image analysis at 4 weeks are above 14.41% and above 28.91% respectively; the absolute values of the change rates of ultraviolet spots and wrinkles shown in facial image acquisition at 2 weeks are above 3.91% and above 15.17% respectively, and the absolute values of the change rates of ultraviolet spots and wrinkles shown in facial image acquisition at 4 weeks are above 4.76% and above 5.17% respectively; and there is no obvious change in the results of skin multiphoton tomography after using the lotion prepared in the application example.

[0198] As can be seen from Application Examples 1-6, the mass parts of the components in the repair composition will affect the comprehensive effect of the rose essential oil; as can be seen from Application Example 1 and Comparative Application Examples 1-10, when a certain component in the repair composition is not added or replaced with other similar components, the effects of the present invention cannot be achieved.

[0199] Finally, it should be noted that the above embodiments are used to illustrate the technical solutions of the present invention rather than to limit the protection scope of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that the technical solutions of the present invention can be modified or equivalently replaced without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A skin barrier repairing composition, characterized in that: The repair composition comprises Rosa centifolia flower extract, rose flower extract, lactobacillus / soy milk fermentation product filtrate, vitamin E, vitamin B5 and sodium hyaluronate.

2. The repair composition according to claim 1, characterized in that The repair composition comprises the following components in parts by weight: 5-15 parts of Rosa centifolia flower extract, 5-15 parts of rose flower extract, 0.5-3.5 parts of lactobacillus / soy milk fermentation product filtrate, 0.0001-0.0003 parts of vitamin E, 0.1-0.4 parts of vitamin B5, and 20-40 parts of sodium hyaluronate.

3. The repair composition according to claim 2, characterized in that The repair composition comprises the following components in parts by weight: 8-12 parts of Rosa centifolia flower extract, 8-12 parts of rose flower extract, 1.5-2.5 parts of lactobacillus / soy milk fermentation product filtrate, 0.00012-0.0002 parts of vitamin E, 0.15-0.3 parts of vitamin B5, and 25-35 parts of sodium hyaluronate.

4. The repair composition according to claim 2, characterized in that Based on the total mass of the repair composition, the mass percentage of the Rosa centifolia flower extract and the rose flower extract is 36-40%.

5. The repair composition according to claim 2, characterized in that: The mass ratio of the Rosa centifolia flower extract to the rose flower extract is 1:(0.8-1.2).

6. Use of the repair composition according to any one of claims 1 to 4 in the preparation of skin care products.

7. A rose essential oil, characterized in that: The rose essential oil comprises the repair composition according to any one of claims 1 to 5.

8. The rose essential oil according to claim 7, characterized in that Calculated based on the mass of the rose essential oil, the mass percentage of the repair composition is 0.1-5%.

9. The rose essential oil according to claim 7, characterized in that The rose essential oil also includes squalane and deionized water.

10. The rose essential oil according to claim 9, characterized in that The rose essential oil comprises the following components in percentage by mass: 0.1-5% of a repairing composition, 15-25% of squalane, and the balance of deionized water.

Images