Preparation method and application of xanthophyll carbon nanodots with whitening and pancreatic cancer resisting functions
By preparing lutein carbon nanodots, the problem of insufficient efficacy and adverse effects of pancreatic cancer treatment was solved, and the growth inhibition and whitening effect on pancreatic cancer cells was achieved, and the effect of regulating blood lipids was also achieved.
Patent Information
- Application Number
- CN202510358248.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-25
- Publication Date
- 2025-06-20
AI Technical Summary
The prior art is difficult to effectively treat pancreatic cancer, especially in patients with middle and late stages. Traditional treatment methods have problems with adverse effects and insufficient efficacy.
By preparing lutein carbon nanodots, using their unique light stability, biocompatibility and antioxidant effects, drugs and skin care products with anti-pancreatic cancer and whitening effects were developed.
Lutein carbon nanodots significantly inhibit the growth of pancreatic cancer cells. By inducing cell cycle arrest, inhibiting cell migration and invasion, and promoting cell apoptosis, they achieve anti-pancreatic cancer effects, and at the same time have the effects of whitening and lightening spots and regulating blood lipid metabolism.
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Figure CN120168438A_ABST
Abstract
Description
Technical Field
[0001] The present invention belongs to carbon dots, and particularly relates to a preparation method and application of lutein carbon nanodots with whitening and anti-pancreatic cancer effects. Background Art
[0002] Pancreatic cancer, this terrifying demon, has always occupied an important position in the spectrum of major diseases in our country. Although the age-standardized incidence rate of pancreatic cancer has shown a gradually stable downward trend in recent years, its shadow still looms large in our lives. For many patients with advanced pancreatic cancer, the golden period for surgical treatment has passed, and the adverse effects of treatment methods such as radiotherapy and chemotherapy are extremely painful. However, in this battle against cancer, traditional Chinese medicine has brought new hope to patients with its unique advantages. It can significantly improve symptoms, enhance the quality of life, control the growth of tumors, and even extend the survival period of patients. The curative effect of traditional Chinese medicine is stable and the side effects are small, enabling patients to regain confidence during the treatment process and feel the hope and vitality of life.
[0003] Carbon dots have excellent water solubility and can easily be compatible with the surrounding environment. At the same time, carbon dots also have excellent light stability and can remain stable even under strong light. More commendably, carbon dots have good biocompatibility, are non-toxic and harmless to organisms, making them have broad application prospects in the biomedical field. The method for preparing carbon dots is simple and easy to implement, and the raw materials are rich in sources, making the production cost of carbon dots low, which is conducive to large-scale application. Compared with traditional metal quantum dots, carbon dots have higher stability and a wider application range. Its emergence has brought a more environmentally friendly and efficient alternative to the field of temperature sensors.
[0004] These advantages of carbon dots make them have broad application prospects in the field of temperature sensors. With the in-depth research, it is believed that carbon dots will play their unique advantages in more fields and bring more convenience and benefits to human production and life. Summary of the Invention
[0005] In order to solve the above technical problems, the present invention provides a preparation method and application of lutein carbon nanodots with whitening and anti-pancreatic cancer effects.
[0006] An application of lutein carbon nanodots of the present invention, wherein the lutein carbon nanodots are used for preparing anti-pancreatic cancer drugs.
[0007] Further, the mass concentration of the lutein carbon nanodots is 10 - 40 μg / mL.
[0008] An application of lutein carbon nanodots of the present invention, wherein the lutein carbon nanodots are used for preparing light spot-removing and whitening skin care products.
[0009] Furthermore, the mass concentration of the lutein carbon nanodots is 10 - 40 μg / mL.
[0010] A preparation method of lutein carbon nanodots with whitening and anti - pancreatic cancer effects according to the present invention is carried out according to the following steps:
[0011] Take lutein and deionized water, mix them evenly to obtain a lutein solution; transfer it into a polytetrafluoroethylene reaction kettle, react at a temperature of 160 - 180 °C for 6 - 8 h, after cooling, centrifuge and filter to obtain lutein carbon nanodots.
[0012] Furthermore, the mass concentration of the lutein carbon nanodots is 10 - 40 μg / mL.
[0013] Furthermore, the centrifugation conditions are centrifuging at a speed of 10000 r / min for 5 min.
[0014] Furthermore, the filtration is carried out with a 0.22 nm filter membrane.
[0015] The present invention has the following beneficial effects:
[0016] The lutein carbon nanodots of the present invention have a growth inhibitory effect on PANC - 1 cells. The lutein carbon nanodots can significantly inhibit the growth of pancreatic cancer PANC - 1 cells. The lutein carbon nanodots achieve the anti - pancreatic cancer effect by inducing cell cycle arrest, inhibiting cell migration and invasion, and promoting cell apoptosis. The carbon nanodots with whitening effect of the present invention can reduce freckles, improve the skin condition, and have the effect of whitening and lightening spots. Through antioxidant and anti - inflammatory effects, it can effectively inhibit melanin production, improve skin brightness and uniformity; and has a certain therapeutic effect on hyperlipidemia. The present invention can regulate the expression of genes related to blood lipid metabolism, and the lutein carbon nanodots can reduce the levels of cholesterol and triglycerides in the blood, contributing to the prevention of cardiovascular diseases. Description of the Drawings
[0017] Figure 1 It is the ultraviolet absorption characteristic diagram of lutein carbon nanodots; Figure A is the ultraviolet absorption characteristic curve diagram of lutein carbon nanodots; Figure B is the photo of lutein carbon nanodots under visible light and ultraviolet light; among them, the photo under visible light (left) and 365 nm ultraviolet light (right) irradiation.
[0018] Figure 2 It is the excitation - dependent characteristic diagram of lutein carbon nanodots;
[0019] Figure 3 It is the infrared absorption spectrum diagram of lutein carbon nanodots;
[0020] Figure 4 It is the fluorescence decay curve diagram of lutein carbon nanodots;
[0021] Figure 5 It is the activity analysis diagram of PANC-1 cells after different administration concentrations of lutein carbon nanodots to PANC-1 cells;
[0022] Figure 6 It is the HE staining analysis diagram. Specific implementation manners
[0023] To make the objectives, technical solutions and advantages of the embodiments of the present invention clearer and more understandable, the spirit of the content disclosed by the present invention will be described in detail below. After any person skilled in the art in the technical field to which the present invention pertains understands the embodiments of the content of the present invention, the techniques taught by the content of the present invention can be changed and modified, which does not deviate from the spirit and scope of the content of the present invention.
[0024] The schematic embodiments of the present invention and their descriptions are used to explain the present invention, but do not limit the present invention.
[0025] Example 1
[0026] A preparation method of lutein carbon nanodots with whitening and anti-pancreatic cancer effects includes the following steps: First, precisely take out 0.02 - 0.06 grams of lutein, and the remaining part is deionized water with a ratio of 1:125. After mixing these two parts evenly, put them into a reaction kettle. Then, put the reaction kettle into an oven, set the temperature to 170 °C, and let them react in such an environment for 7 hours. After the reaction is completed, carefully suck out the medicine with a syringe, and then put it into a centrifuge tube. Next, put the centrifuge tube into a centrifuge, set the centrifugation time to 5 minutes, and the rotation speed to 10,000 r / min. After centrifugation, filter with a 0.22 nm filter membrane, and put the filtered medicine into a refrigerator, with the storage temperature set to -4 °C.
[0027] The ultraviolet absorption characteristic diagram of lutein carbon nanodots is as Figure 1 shown. It can be seen from the figure that lutein and its carbon nanodots are described by an absorption curve. In the figure, the wavelength is the abscissa and the absorbance is the ordinate. As the wavelength increases, the absorbance gradually decreases. This means that the light absorption ability of lutein and its carbon nanodots weakens under the irradiation of light with a longer wavelength. Lutein has a strong ultraviolet absorption peak at about 250 nm, and lutein carbon nanodots have ultraviolet absorption at 220 nm. The aqueous solution of lutein aqueous extract carbon dots is yellow under visible light irradiation, which may be due to the absorption of visible light by lutein and its carbon nanodots. Under the irradiation of ultraviolet light at 365 nm, the aqueous solution of lutein aqueous extract carbon dots is bright blue. This may be due to the photochemical reaction of lutein and its carbon nanodots under ultraviolet light irradiation.
[0028] The excitation-dependent characteristic diagram of lutein carbon nanodots is as Figure 2As shown, lutein carbon dots exhibit an obvious fluorescence excitation dependence phenomenon, which means that their fluorescence emission characteristics strongly depend on the wavelength of the excitation light source. Through experimental research, the optimal excitation wavelength of the lutein carbon dots of the present invention is 370 nm, and the optimal emission wavelength is 450 nm.
[0029] The infrared absorption spectrum of lutein carbon dots is as Figure 3 shown Figure 3 showing that obvious absorption peaks appear around 3000 cm -1 due to the presence of -OH bonds in their structure. The -OH bond (hydroxyl group) is a polar bond with strong absorption ability, especially in the infrared region. Therefore, the absorption peak near 3000 cm -1 can be attributed to the vibration of the hydroxyl group in lutein carbon dots. Additionally, obvious doublets appear around 1600 cm -1 due to the presence of C=C double bonds in the structure of lutein carbon dots. The C=C double bond is one of the characteristics of olefin compounds, and the absorption peak caused by its vibration shows an obvious doublet phenomenon in the infrared region.
[0030] The fluorescence decay curve of lutein carbon dots is as Figure 4 shown. Lutein carbon dots have two fluorescence centers in terms of fluorescence properties, which may be related to factors such as their structure, composition, and surface modification. By fitting the fluorescence decay curve of lutein carbon dots with a second-order decay exponential function, two different fluorescence lifetimes (τ) can be obtained. Through calculation, the average fluorescence lifetime is 2.99 ns.
[0031] Example 2
[0032] Analysis of the anti-pancreatic cancer activity of lutein carbon dots
[0033] To evaluate the in vitro cytotoxicity of lutein carbon dots on PANC-1 cells, the CCK-8 assay was used in this example. First, PANC-1 cells were cultured to the logarithmic growth phase to ensure that the cell density was within an appropriate range of 3000 - 5000 cells / well. Then, the cells were transferred to a 96-well cell culture plate and continued to be cultured in an incubator containing 5% CO2 at 37°C.
[0034] When the cells were completely attached to the culture flask wall, 20 μL of lutein carbon dots with different concentrations (0, 10, 20, 30, 40 μg / mL) were added to each well. After incubating the cells with lutein carbon dots for 48 hours, 20 μL of CCK-8 reagent was added to each well.
[0035] Put the 96-well cell culture plate containing CCK-8 reagent back into the incubator and incubate for 40 minutes. Subsequently, measure the optical density (OD) value at a wavelength of 450 nm using a microplate reader. Calculate the viability of PANC-1 cells based on the measured OD value.
[0036] Result analysis: According to Figure 5 , it can be seen that as the administration concentration of lutein carbon dots increases, the activity of PANC-1 cells gradually decreases. When the administration concentration reaches 40 μg / mL, the cell activity drops to the lowest, approximately 60%.
[0037] These results indicate that lutein carbon dots have an inhibitory effect on the growth of PANC-1 cells and have a certain anti-pancreatic cancer effect. In subsequent experiments, the mechanism of action, optimal action concentration, and possible clinical application value of lutein carbon dots can be further explored.
[0038] Verification of lutein carbon dots in mouse cancer repair: To explore the potential of lutein carbon dots in cancer treatment, first, lutein carbon dots were synthesized and their structure and properties were verified by a series of characterization techniques. Subsequently, these nanoparticles were applied to the skin of damaged mice to observe their repair effect on cancer damage.
[0039] Example 3
[0040] Activity test of the whitening effect of lutein carbon dots:
[0041] 1. First, conduct skin tests on 7 volunteers to ensure that they have no abnormal conditions such as skin allergies.
[0042] 2. After the experiment starts, the volunteers apply the test sample on their faces every morning and evening, and the application area should be uniform. During the experiment, the subjects should not apply other cosmetics on the experimental site to ensure the accuracy of the experimental results.
[0043] 3. Test the volunteers respectively after 0 days, 14 days, and 30 days of continuous use of the test sample. The test contents include:
[0044] a. Observation of skin condition: Observe the changes in skin color, luster, fineness, etc.
[0045] b. Measurement of skin moisture content: Use a professional instrument to measure the skin moisture content to evaluate the effect of the test sample on skin moisturization.
[0046] c. Measurement of skin oil secretion: Use a professional instrument to measure the skin oil secretion amount to evaluate the effect of the test sample on controlling skin oil secretion.
[0047] d. Questionnaire survey: Distribute questionnaires to collect the usage feelings of volunteers during the experiment, such as comfort, ease of use, smell, etc.
[0048] 4. Data collection and analysis: Statistically analyze the experimental data to evaluate the efficacy and safety of the test samples.
[0049] 5. Compile an experimental report: Organize the experimental data and results, write an experimental report, and summarize the advantages and disadvantages of the test samples.
[0050] Test results:
[0051]
[0052] According to the usage feelings of the subjects, it shows that the carbon nanodots with whitening effect of the present invention can reduce freckles, improve the skin condition, and have the effect of whitening and lightening spots.
[0053] Example 4
[0054] Evaluate the repair effect of lutein carbon nanodots on the subcutaneous tissue of hyperlipidemic mice.
[0055] 1. Experimental design:
[0056] Model establishment: Replicate a hyperlipidemic mouse model by feeding a high-sugar and high-fat diet.
[0057] Animal grouping: Randomly divide the mice into three groups, with 3 mice in each group to ensure the reliability of the experimental results.
[0058] Drug administration method: Subcutaneous administration, which may mean injecting the drug directly into the subcutaneous tissue of the mice.
[0059] Control group: Use normal saline, which is usually used as a control without therapeutic effect.
[0060] Experimental group: Use lutein aqueous solution and its carbon nanodots respectively to evaluate the effects of these two treatments on the hyperlipidemia model.
[0061] Experimental period: The experiment lasts for 14 days, during which the physiological changes of the mice can be observed.
[0062] 2. Follow-up steps:
[0063] HE staining analysis: HE staining of skin tissue can be used to observe tissue structure and cell morphology, so as to evaluate the pathological changes of the subcutaneous tissue of each group of mice.
[0064] Statistical analysis: Statistically analyze the experimental data to ensure the reliability and validity of the results.
[0065] Repeat the experiment: In order to verify the repeatability of the results, more experiments should be carried out or the sample size should be expanded.
[0066] Through HE staining, the morphological structures of cells and tissues can be observed under a microscope, such as Figure 6 shown. Compared with the blank control group and the lutein group, improvement in the tumor tissue structure was observed in the lutein carbon nanodot group, with more orderly cell arrangement and clearer tumor boundaries. This indicates that lutein carbon nanodots have a certain effect on the treatment of pancreatic cancer.
Claims
1. An application of lutein carbon nanodots, characterized in that The lutein carbon nanodots are used for preparing drugs against pancreatic cancer.
2. The use of lutein carbon nanodots according to claim 1, characterized in that The mass concentration of the lutein carbon nanodots is 10-40 μg / mL.
3. An application of lutein carbon nanodots, characterized in that The lutein carbon nanodots are used to prepare spot-lightening and whitening skin care products.
4. The use of lutein carbon nanodots according to claim 1, characterized in that The mass concentration of the lutein carbon nanodots is 10-40 μg / mL.
5. A method for preparing lutein carbon nanodots with whitening and anti-pancreatic cancer properties, characterized in that It is carried out according to the following steps: Lutein and deionized water are mixed evenly to obtain a lutein solution; the solution is transferred into a polytetrafluoroethylene reactor and reacted at a temperature of 160-180° C. for 6-8 hours. After cooling, the solution is centrifuged and filtered to obtain lutein carbon nanodots.
6. The method for preparing the lutein carbon nanodots with whitening and anti-pancreatic cancer properties according to claim 1, characterized in that The mass concentration of the lutein carbon nanodots is 10-40 μg / mL.
7. The method for preparing lutein carbon nanodots with whitening and anti-pancreatic cancer properties according to claim 1, characterized in that The centrifugal condition is 10000r / min speed and centrifugation for 5min.
8. The method for preparing the lutein carbon nanodots with whitening and anti-pancreatic cancer properties according to claim 1, characterized in that The filtration was performed using a 0.22 nm filter membrane.