Traditional Chinese medicine composition for promoting blood circulation to remove blood stasis and activating meridians to stop pain and identification method thereof

Abstract

The invention discloses a traditional Chinese medicine composition for promoting blood circulation to remove blood stasis and activating meridians to stop pain and an identification method of the traditional Chinese medicine composition, and belongs to the field of traditional Chinese medicine, the traditional Chinese medicine composition is prepared from 9-30 parts of radix astragali, 6-12 parts of cortex moutan, 5-10 parts of peach kernels, 3-10 parts of flos carthami, 6-12 parts of radix angelicae sinensis, 6-10 parts of rhizoma cyperi, 5-10 parts of lumbricus and 3-10 parts of rhizoma chuanxiong. Qi flourishing, blood circulation promoting, stasis removing and collateral dredging are achieved; angelica sinensis is used as a minister, and can promote blood circulation and nourish blood, so that the traditional Chinese medicine has the effect of dispersing blood stasis without hurting blood; cortex moutan, ligusticum wallichii, peach kernel and safflower are used as assistants to promote blood circulation and remove blood stasis of Chinese angelica, lumbricus is used as assistants to clear and activate the channels and collaterals, rhizoma cyperi is used as assistants to regulate qi and relieve pain, and a qi-tonifying medicine is matched with a blood-activating medicine, so that qi is flourishing to promote blood circulation without hurting vital energy, and the effects of tonifying qi, activating blood and activating meridians to relieve pain are achieved.

Description

Technical Field

[0001] The present invention relates to the field of traditional Chinese medicine, and particularly to a traditional Chinese medicine composition for promoting blood circulation to remove blood stasis, dredging collaterals and relieving pain, and a method for identifying the same. Background Art

[0002] Stroke, that is, cerebrovascular accident, also known as cerebrovascular accident, is mainly divided into ischemic stroke, cerebral hemorrhage, subarachnoid hemorrhage and unclassified stroke.

[0003] The etiology and pathogenesis of stroke are relatively complex and involve multiple factors. The main risk factors are hypertension, abnormal glucose metabolism, dyslipidemia, asymptomatic carotid atherosclerosis, unhealthy lifestyle, vascular malformations, etc., which lead to vascular stenosis or even blockage, affecting the normal flow of blood, and then causing insufficient cerebral blood supply or local tissue necrosis. In terms of treatment, mainly intervene in the modifiable factors among the risk factors, such as controlling blood pressure, improving blood sugar, adjusting blood lipids and changing unhealthy lifestyle, etc. Thrombolysis, surgery and other treatments can be selected in the acute phase. The treatment of stroke needs to comprehensively consider the specific condition and etiology of the patient and formulate an individualized treatment plan. However, some patients cannot tolerate the relevant drug treatment due to the adverse reactions of western medicine, so there are certain risks in treatment. Stroke is a general term in traditional Chinese medicine for acute cerebrovascular diseases. It is a class of diseases mainly characterized by sudden fainting, unconsciousness, accompanied by deviation of the mouth and eyes, speech disorders and hemiplegia. Traditional Chinese medicine believes that the occurrence of stroke is mostly based on internal injury and accumulation, and is triggered by overwork, emotional discomfort, overeating and drinking alcohol or external pathogen invasion, etc., which causes disharmony between the zang-fu organs and yin-yang, blood following qi reversal, liver yang soaring, internal wind swirling, accompanied by phlegm and fire, running horizontally through the meridians, blocking the shenqiao, and thus sudden fainting and hemiplegia occur. At present, there are many drugs for treating stroke sequelae on the market, and the curative effects are different.

[0004] The preparation of the present formulation is improved for the above-mentioned existing defects and evolved from the clinical decoction for many years. During the clinical use of the decoction, due to its good therapeutic effect and small side effects, it can be better promoted and applied. Summary of the Invention

[0005] In order to solve the problems mentioned in the above background art, the present invention provides a traditional Chinese medicine composition for promoting blood circulation to remove blood stasis, dredging collaterals and relieving pain, and a method for identifying the same.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions: A preparation method of a traditional Chinese medicine composition for promoting blood circulation to remove blood stasis, dredging collaterals and relieving pain, comprising the following steps: (1) Pretreatment of medicinal materials: Weigh eight traditional Chinese medicinal materials of Astragalus membranaceus, Angelica sinensis, Paeonia suffruticosa Andr., Cyperus rotundus L., Prunus persica (L.) Batsch, Pheretima aspergillum (E. Perrier), Carthamus tinctorius L. and Ligusticum chuanxiong Hort. respectively after selecting and removing impurities, and then wash and set aside; (2) Medicinal material extraction: Add 8 times the amount of water, soak for 1 hour, decoct for 2 hours, filter, and reserve the filtrate; add 6 times the amount of water to the residue, decoct for another 2 hours and then filter. Combine the filtrate obtained from the residue with the previous filtrate, and discard the residue. (3) Filtrate concentration: Concentrate the combined filtrate to a relative density of 1.15 - 1.20 to obtain an extract. (4) Extract drying: Dry the extract at a temperature of 80 ± 5 °C until the moisture content ≤ 5.0% to obtain a dry extract. (5) Dry extract pulverization: Pulverize the dry extract to form a dry extract powder. (6) Mixing: Weigh microcrystalline cellulose, cross-linked povidone and mix them evenly with the dry extract powder. (7) Granulation: Prepare a soft material with 80% ethanol, granulate through a 16-mesh sieve, dry at 70 ± 5 °C, control the moisture content: 3 - 5%, and size the granules through a 14-mesh sieve. (8) Total mixing: Weigh sodium carboxymethyl starch and silicon dioxide, and mix them evenly with the granules. (9) Tablet pressing and bottling: Debug the tablet press, press the mixture after total mixing, and control the tablet weight at 0.8 g ± 5%.

[0007] Preferably, the temperature for filtrate concentration is controlled at 60 °C.

[0008] Preferably, the dry extract is pulverized to form an 80-mesh dry extract powder.

[0009] On the other hand, the present invention also provides a method for identifying astragalus in a traditional Chinese medicine composition for promoting blood circulation to remove blood stasis and dredging collaterals to relieve pain. The identification method is as follows: Take 10 tablets of this product, grind them finely, add 50 ml of methanol, ultrasonically treat for 40 minutes, let it cool, filter, evaporate the filtrate to dryness, dissolve the residue in 30 ml of water, shake and extract with water-saturated n-butanol twice, 30 ml each time. Combine the n-butanol solutions, wash with 1% sodium hydroxide solution twice, 20 ml each time, and then wash with water saturated with n-butanol solution until neutral. Take the n-butanol solution and evaporate it to dryness, dissolve the residue in 1 ml of methanol to obtain a test solution. Additionally, take astragaloside reference substance, add methanol to make a solution containing 1 mg per 1 ml as a reference solution. According to the thin-layer chromatography test, absorb 5 μl of each of the above two solutions, respectively spot them on the same silica gel G thin-layer plate, use the lower layer solution of chloroform - methanol - water (13:7:2) as the developing agent, develop, take out, dry in the air, spray with 10% sulfuric acid ethanol solution, heat at 105 °C until the spots are clearly visible. In the test solution chromatogram, at the corresponding position to the reference solution chromatogram, there are spots of the same color. Place it under an ultraviolet lamp for inspection. In the test solution chromatogram, at the corresponding position to the reference solution chromatogram, there are fluorescent spots of the same color.

[0010] The present invention also provides a method for identifying safflower in a traditional Chinese medicine composition for promoting blood circulation to remove blood stasis, dredging collaterals and relieving pain, which is characterized in that the identification method consists of the following steps: Take 10 tablets of this product and make them into powder, then use ethanol to extract the active ingredients from the medicinal materials, filter, evaporate the filtrate to dryness, dissolve the residue in 2 ml of ethanol to make a test solution. Select adsorbent silica gel G and evenly coat it on a glass plate or other support to form a thin layer. Use a capillary tube or a spotting device to drop the test solution and the reference solution on the starting line of the thin layer plate respectively. After the development is completed, take out the thin layer plate, dry it, and then select the corresponding developer according to the properties of the separated substances for spraying and coloring, or use an ultraviolet lamp to detect the spots of the separated substances.

[0011] Compared with the prior art, the beneficial effects of the present invention are as follows: 1. The formula of the traditional Chinese medicine composition of the present invention is reasonable, and it has a significant curative effect on numbness of hands and feet, weakness of limbs, deviation of the mouth and eyes, and unclear speech in the recovery period of sequelae of stroke. It can relieve the discomfort of patients in a short time, and the treatment effect is remarkable.

[0012] 2. The composition of the present invention is prepared from all traditional Chinese medicines, with a simple formula. It is a commonly used medicinal material in traditional Chinese medicine, with small toxic and side effects, high safety, and strong practicability.

[0013] 3. At present, there are many drugs for treating sequelae of stroke on the market, and their curative effects vary. This preparation is evolved from the clinical decoction of our hospital for many years. This drug has a good treatment effect and small side effects. In this prescription, astragalus membranaceus is used as the monarch drug, and its dosage is increased to greatly tonify the vital energy of the spleen and stomach, so that qi is strong and blood circulates, stasis is removed and collaterals are dredged; angelica sinensis is used as the minister drug, which is good at promoting blood circulation and can also nourish blood, so it has the wonderful effect of promoting blood stasis without damaging blood; moutan cortex, chuanxiong, peach kernel, and safflower are used as adjuvant drugs to assist angelica sinensis in promoting blood circulation and removing blood stasis. Then, earthworm is used to dredge the meridians and collaterals, and cyperus rotundus is used to regulate qi and relieve pain. By using a large amount of qi-tonifying drugs and combining them with blood-activating drugs, qi is strong and blood circulates, and promoting blood circulation and removing blood stasis without damaging healthy qi, so as to jointly achieve the effects of tonifying qi and promoting blood circulation, dredging collaterals and relieving pain. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. Obviously, the following drawings are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0015] Figure 1 : Thin layer identification chromatogram of astragaloside IV (daylight); Figure 2 : Thin layer identification chromatogram of astragaloside IV (fluorescence); Figure 3 : Thin layer identification chromatogram of safflower; Figure 4:Thin-layer chromatographic identification of astragaloside Figure 1 Daylight (0 months); Figure 5 :Thin-layer chromatographic identification of astragaloside Figure 1 Fluorescence (0 months); Figure 6 :Thin-layer chromatographic identification of astragaloside Figure 1 Daylight (3 months); Figure 7 :Thin-layer chromatographic identification of astragaloside Figure 1 Fluorescence (3 months); Figure 8 :Thin-layer chromatographic identification of astragaloside Figure 1 Daylight (6 months); Figure 9 :Thin-layer chromatographic identification of astragaloside Figure 1 Fluorescence (6 months); Figure 10 :Thin-layer chromatographic identification of astragaloside Figure 1 Daylight (9 months); Figure 11 :Thin-layer chromatographic identification of astragaloside Figure 1 Fluorescence (9 months); Figure 12 :Thin-layer chromatographic identification of astragaloside Figure 1 Daylight (12 months); Figure 13 :Thin-layer chromatographic identification of astragaloside Figure 1 Fluorescence (12 months); Figure 14 :Thin-layer chromatographic identification of astragaloside Figure 1 Daylight; Figure 15 :Thin-layer chromatographic identification of astragaloside Figure 1 Fluorescence. Detailed implementation manners

[0016] The technical solutions of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0017] Unless otherwise specified, the raw materials used in the present invention are all conventional products purchased from the market.

[0018] Example 1: The ratio of the traditional Chinese medicine composition is as follows: 9 - 30 parts of Astragalus membranaceus, 6 - 12 parts of Cortex Moutan, 5 - 10 parts of Prunus persica, 3 - 10 parts of Carthamus tinctorius, 6 - 12 parts of Angelica sinensis, 6 - 10 parts of Cyperus rotundus, 5 - 10 parts of Pheretima aspergillum, 3 - 10 parts of Ligusticum wallichii. The mass ratio of Astragalus membranaceus, Angelica sinensis, Cortex Moutan, Cyperus rotundus, Prunus persica, Pheretima aspergillum, Carthamus tinctorius, Ligusticum wallichii is 44:13.2:13.2:13.2:11:11:6.6:6.6.

[0019] A preparation method of a traditional Chinese medicine composition for promoting blood circulation to remove blood stasis and dredging collaterals to relieve pain, comprising the following steps: (1) Weigh eight traditional Chinese medicine materials of Astragalus membranaceus, Angelica sinensis, Cortex Moutan, Cyperus rotundus, Prunus persica, Pheretima aspergillum, Carthamus tinctorius, and Ligusticum wallichii respectively after selecting and removing impurities, then wash and set aside; (2) Add 8 times the amount of water, soak for 1 hour, decoct for 2 hours, filter, and reserve the filtrate; add 6 times the amount of water to the medicinal residues, decoct for another 2 hours and then filter, combine the filtrate obtained from the medicinal residues with the previous filtrate, and discard the medicinal residues; (3) Control the concentration temperature of the filtrate at 60 °C, concentrate the combined filtrate to a relative density of 1.15 - 1.20 to obtain an extract; (4) Dry the extract at a temperature of 80 ± 5 °C until the moisture content ≤ 5.0% to obtain a dry extract; (5) Crush the dry extract to form a dry extract powder of 80 meshes; (6) Weigh microcrystalline cellulose and crospovidone, and mix them evenly with the dry extract powder; (7) Prepare soft materials with 80% ethanol, granulate with a 16 - mesh sieve, dry at 70 ± 5 °C, control the moisture content: 3 - 5%, and size - screen with a 14 - mesh sieve; (8) Weigh sodium carboxymethyl starch and silicon dioxide, and mix them evenly with the granules; (9) Debug the tablet press, tablet the mixed mixture, and control the tablet weight at 0.8 g ± 5%.

[0020] Example 2: A method for identifying the components of a traditional Chinese medicine composition, providing the following two component identifications: Identification method of Astragalus membranaceus in a traditional Chinese medicine composition for promoting blood circulation to remove blood stasis and dredging collaterals to relieve pain. Take 10 tablets of this product, grind them finely, add 50 ml of methanol, ultrasonically treat for 40 minutes, let it cool, filter, evaporate the filtrate to dryness, dissolve the residue in 30 ml of water, shake and extract with water-saturated n-butanol twice, 30 ml each time. Combine the n-butanol solutions, wash with 1% sodium hydroxide solution twice, 20 ml each time, then wash with water saturated with n-butanol solution until neutral. Take the n-butanol solution and evaporate it to dryness. Dissolve the residue in 1 ml of methanol to prepare the test solution. Additionally, take astragaloside IV reference substance, dissolve it in methanol to prepare a solution containing 1 mg per 1 ml as the reference solution. According to the thin-layer chromatography test, absorb 5 μl of each of the above two solutions and spot them on the same silica gel G thin-layer plate respectively. Use the lower layer solution of chloroform-methanol-water (13:7:2) as the developing agent, develop, take out, dry, spray with 10% sulfuric acid ethanol solution, heat at 105 °C until the spots are clearly visible. In the test solution chromatogram, at the position corresponding to the reference solution chromatogram, there are spots of the same color. Place it under an ultraviolet lamp for inspection. In the test solution chromatogram, at the position corresponding to the reference solution chromatogram, there are fluorescent spots of the same color.

[0021] Identification method of Carthamus tinctorius in a traditional Chinese medicine composition for promoting blood circulation to remove blood stasis and dredging collaterals to relieve pain. Take 10 tablets of this product and make them into powder. Then extract the active ingredients from the medicinal materials with ethanol, filter, evaporate the filtrate to dryness, dissolve the residue in 2 ml of ethanol to prepare the test solution. Select adsorbent silica gel G and evenly coat it on a glass plate or other supports to form a thin layer. Use a capillary or spotting device to drop the test solution and the reference solution on the starting line of the thin-layer plate respectively. After development is completed, take out the thin-layer plate, dry it, and select the corresponding developer to spray and develop according to the properties of the separated substances, or use an ultraviolet lamp to detect the spots of the separated substances. As mentioned above, it is only the preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present invention, according to the technical solution and inventive concept of the present invention, makes equivalent substitutions or changes, and all should be covered within the protection scope of the present invention.

Claims

1. A Chinese medicine composition for promoting blood circulation, removing blood stasis, unblocking meridians and relieving pain, characterized in that: The invention comprises the following components in parts by weight: 9-30 parts of astragalus, 6-12 parts of peony bark, 5-10 parts of peach kernel, 3-10 parts of safflower, 6-12 parts of angelica, 6-10 parts of cyperus, 5-10 parts of earthworm and 3-10 parts of ligusticum chuanxiong.

2. A Chinese medicine composition for promoting blood circulation, removing blood stasis, unblocking meridians and relieving pain according to claim 1, characterized in that: The mass ratio of astragalus, angelica, peony bark, cyperus, peach kernel, earthworm, safflower and ligusticum chuanxiong is 44: 13.2：13.2：13.2：11：11：6.6：6.6。 3. The method for preparing the Chinese medicine composition for promoting blood circulation, removing blood stasis, dredge meridians and relieving pain according to claim 1 or 2, characterized in that: The method comprises the following steps: (1) Pretreatment of medicinal materials: Take eight kinds of Chinese medicinal materials, including astragalus, angelica, peony bark, cyperus, peach kernel, earthworm, safflower, and ligusticum chuanxiong, select and remove impurities, weigh them, and then wash them for later use; (2) Extraction of medicinal materials: add 8 times the amount of water, soak for 1 hour, decoct for 2 hours, filter, and reserve the filtrate; add 6 times the amount of water to the medicinal residue, decoct for another 2 hours, filter, and combine the obtained medicinal residue filtrate with the above filtrate, and discard the medicinal residue; (3) Filtrate concentration: Concentrate the combined filtrate to a relative density of 1.15-1.20 to obtain an extract; (4) Extract drying: Dry the extract at a temperature of 80±5°C until the moisture content is ≤5.0% to obtain a dry extract; (5) Dry paste crushing: crush the dry paste to form dry paste powder; (6) Mixing: weigh microcrystalline cellulose, cross-linked polyvinylpyrrolidone and dry paste powder and mix them evenly; (7) Granulation: Use 80% ethanol to make soft material, granulate with 16-mesh sieve, dry at 70±5℃, control moisture: 3-5%, and granulate with 14-mesh sieve; (8) Mixing: Weigh sodium carboxymethyl starch and silicon dioxide, and mix them evenly with the granules; (9) Tableting and bottling: Debug the tablet press and make tablets of the mixture after total mixing, and control the tablet weight to 0.8g±5%.

4. The method for preparing the Chinese medicine composition for promoting blood circulation, removing blood stasis, unblocking meridians and relieving pain according to claim 3, characterized in that: The filtrate concentration temperature was controlled at 60°C.

5. The method for preparing the Chinese medicine composition for promoting blood circulation, removing blood stasis, unblocking meridians and relieving pain according to claim 3, characterized in that: The dry paste is crushed to form 80 mesh dry paste powder.

6. The method for identifying Astragalus in the Chinese medicine composition for promoting blood circulation, removing blood stasis, dredge meridians and relieving pain according to claim 1, characterized in that The identification method consists of the following steps: take 10 tablets of the product, grind them into powder, add 50 ml of methanol, perform ultrasonic treatment for 40 minutes, cool, filter, evaporate the filtrate to dryness, add 30 ml of water to dissolve the residue, add saturated n-butanol to shake and extract twice, 30 ml each time, combine the n-butanol solution, add 1% sodium hydroxide solution to wash twice, 20 ml each time, then wash with water saturated with n-butanol solution until neutral, take the n-butanol solution and evaporate it to dryness, add 1 ml of methanol to dissolve the residue, and use it as the test solution.

7. The method for identifying Astragalus in the Chinese medicine composition for promoting blood circulation, removing blood stasis, dredge meridians and relieving pain according to claim 6, characterized in that: It also includes taking another astragaloside IV reference substance, adding methanol to make a solution containing 1 mg per 1 ml as the reference substance solution. According to the thin layer chromatography test, 5 μl of each of the above two solutions are respectively spotted on the same silica gel G thin layer plate, and the lower layer solution of chloroform-methanol-water (13:7:2) is used as the developing agent, developing, taking out, drying, spraying with 10% sulfuric acid ethanol solution, heating at 105°C until the spots are clearly colored, and the same color spots appear in the chromatogram of the test substance at the corresponding position of the chromatogram of the reference substance, and the test substance chromatogram is placed under ultraviolet light for inspection, and the same color fluorescent spots appear in the chromatogram of the test substance at the corresponding position of the chromatogram of the reference substance.

8. The method for identifying safflower in the Chinese medicine composition for promoting blood circulation, removing blood stasis, dredge meridians and relieving pain according to claim 1, characterized in that: The identification method consists of the following steps: 10 tablets of the product are made into powder, and then the active ingredients in the medicinal material are extracted using ethanol, filtered, the filtrate is evaporated to dryness, and the residue is dissolved by adding 2 ml of ethanol to serve as the test solution.

9. The method for identifying safflower in the Chinese medicine composition for promoting blood circulation, removing blood stasis, dredge meridians and relieving pain according to claim 8, characterized in that: The method also includes selecting an adsorbent silica gel G and coating it evenly on a glass plate or other support to form a thin layer, using a capillary or a spotter to drop the test solution and the reference solution on the starting line of the thin layer plate respectively, and after the development is completed, taking out the thin layer plate, and after drying, selecting a corresponding color developer to spray for color development according to the properties of the separated substance, or using an ultraviolet lamp to detect the spots of the separated substance.

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