LED illumination equipment and method for repairing damaged endometrium of mouse by using LED illumination equipment

Abstract

The invention relates to the technical field of mouse endometrium repair, and discloses an LED illumination device and a method for repairing damaged mouse endometrium through the LED illumination device, and the method comprises the following steps: S1, experiment grouping; s2, constructing an injured endometrial mouse model; s3, carrying out an illumination experiment; s4, evaluating the endometrial repair effect; the method comprises the following steps: S1, randomly dividing purchased mice into a normal group A, an injury group B, an irradiation group C and an irradiation group D; s2, constructing an injured endometrial mouse model by pouring absolute ethyl alcohol into the uterine cavity of the mouse; s3, performing illumination treatment on the mouse by using self-made LED illumination equipment; and S4, evaluating the repair effect of LED illumination on the damaged endometrium by checking the pregnancy rate and the average litter size of the mouse, and evaluating the recovery condition of the endometrium morphology and function in combination with histological detection. The invention has the advantages of small trauma, fast recovery, substantial reduction of infection and adhesion risks, and promotion of injury repair.

Description

Technical Field

[0001] The present invention relates to the technical field of mouse endometrial repair, and specifically to an LED lighting device and a method for repairing damaged endometrium of mice using the same. Background Art

[0002] The endometrium is crucial for successful embryo implantation and pregnancy maintenance. Endometrial injury is one of the important causes of infertility and repeated implantation failure. Once the structure and function of the endometrium are damaged, it will not only reduce the embryo implantation ability, but may also trigger a series of complications, seriously affecting the fertility outcome of patients. At present, the repair methods for endometrial injury mainly include drug treatment and surgical intervention. Drug treatment usually uses estrogen, progesterone, non-steroidal anti-inflammatory drugs, etc. These drugs improve the thickness and function of the endometrium by regulating hormone levels and reducing inflammation. Surgical intervention includes hysteroscopic surgery, which is used to directly remove diseased tissues and repair the endometrium. However, although these methods can improve the endometrial condition to a certain extent, there are significant limitations. Although drug treatment has a certain effect on regulating hormone levels and reducing inflammation, long-term use of hormonal drugs may cause side effects such as weight gain, mood swings, increased risk of thrombosis, etc., and there is also the problem of drug dependence. Once the drug is stopped, the endometrial condition may deteriorate rapidly. Although surgical treatment can directly remove diseased tissues, it is more traumatic, has a long postoperative recovery period, and there are risks of infection, adhesion, and further injury. In short, neither drug nor surgical treatment can completely solve the problem of endometrial injury.

[0003] LED (light-emitting diode) phototherapy, as a non-invasive physical therapy method without toxic side effects, has shown potential application value in the field of medical health. Research shows that LED lights of different wavelengths have unique biological effects. For example, green light can effectively stimulate the proliferation and migration of endothelial cells, which is crucial for angiogenesis and wound healing processes. White light inhibits the activity of cancer cells by affecting the p53 and MAPK signal transduction pathways in cells, providing a new research direction for cancer treatment. The mechanism of action of blue light is closely related to oxidative stress and inflammation regulation. It is found that blue light can induce oxidative stress response in subcutaneous preadipocytes of cattle, thereby regulating the inflammatory process. Narrow-band LED light in the red to infrared band has shown great potential in the application of the medical field. Phototherapy in this band has a significant impact on the wound healing process. It promotes wound healing by reducing inflammatory cell infiltration, promoting the proliferation of fibroblasts, stimulating angiogenesis and granulation tissue formation, and increasing collagen synthesis. It is found that red light can enhance the expression of transforming growth factor β (TGF-β) protein and increase the density of collagen fibers, thus promoting tissue healing and reconstruction. In terms of neurological diseases, it may improve cognitive dysfunction and neurodegenerative changes.

[0004] LED light also plays an important role in the reproductive system. Research has shown that green light and blue light can significantly affect the ovarian maturation of yellowtail damselfish by increasing the expression of vitellogenin and estrogen receptor (ER), improving the gonadosomatic index and the level of estradiol (E2) in plasma

[18] . These two lights can also regulate the E2 level in chickens, regulate ER and progesterone receptor (PR) in the ovary, and thus effectively increase the total number of eggs laid and the egg production rate during the peak period. In addition, studies have found that the combination of red light and green light can effectively increase the reproductive hormone level, ovarian weight and follicle number of laying hens, and further promote the egg production rate. Some research indicates that red light has a more significant effect on the ovarian maturation and oocyte maturation of yellowtail damselfish than green light or blue light, suggesting that red light may play a key role in activating cell processes related to reproduction. Research on zebrafish further reveals that red light may affect the gonadosomatic index by inducing oxidative damage, and achieve this by reducing the level and activity of antioxidant enzymes and the expression of the antioxidant gene Nrf2. In the field of clinical treatment, near-infrared photoimmunotherapy (NIR-PIT), a new type of photodynamic therapy that combines photosensitizers with antibody complexes, has shown great potential in the treatment of ovarian cancer, providing a new approach for tumor treatment. There is no report on the repair effect of LED light on damaged endometrium.

[0005] In view of the above problems, we provide an LED lighting device and a method for repairing damaged endometrium in mice. Summary of the Invention

[0006] (1) Technical Problems to be Solved

[0007] In view of the deficiencies of the prior art, the present invention provides an LED lighting device and a method for repairing damaged endometrium in mice, solving the problems in the above background technology.

[0008] (2) Technical Solutions

[0009] To achieve the purpose of having less trauma, a short recovery period, significantly reducing the risks of infection and adhesion, and promoting injury repair, the present invention provides the following technical solutions:

[0010] An LED lighting device and a method for repairing damaged endometrium in mice, comprising the following steps:

[0011] S1. Experimental grouping: Purchase several mice, check their health conditions, and randomly divide the mice into four groups, namely normal group A, injury group B, irradiation group C, and irradiation group D;

[0012] S2. Establish a mouse injury model: Establish a mouse endometrial injury model by perfusing absolute ethanol into the uterine cavity of mice.

[0013] S3. Light irradiation experiment: Use a self-made LED light irradiation device to irradiate mice. Take the normal group A and the injury group B as the control groups, maintaining the same breeding environment. The irradiation groups C and D are respectively irradiated with LED lights of different wavelengths, with each light irradiation lasting 5 - 10 minutes and continuous irradiation for 10 - 15 days, spanning 3 estrus cycles for the experiment, simulating 3 treatment courses.

[0014] S4. Effect evaluation: Detect the repair effect of LED light irradiation on the injured endometrium by evaluating the pregnancy rate and average litter size of mice, and simultaneously use histological methods to evaluate the morphological and functional recovery of the endometrium.

[0015] Preferably, the LED light irradiation device in step S3 includes an installation shell, a fan assembly and a light irradiation assembly installed on the installation shell. The light irradiation assembly includes a multi - spectral LED light source. The multi - spectral LED light source is encapsulated on a flexible circuit board and installed on the installation shell, and the flexible circuit board is connected to a controller.

[0016] The multi - spectral LED light source is used to generate light of specific wavelengths and intensities for the light irradiation experiment on mice.

[0017] Preferably, the multi - spectral LED light source includes several LED lamp beads, and the LED lamp beads are used to generate light of specific wavelengths and intensities.

[0018] The several LED lamp beads distributed inside the installation shell are arranged in a ring for irradiating mice.

[0019] Preferably, the fan assembly includes a fan and a fan power supply. A fan is installed on the installation shell, and the fan is connected to the fan power supply.

[0020] Preferably, the controller is used to control the turning on or off of the light source, select the working wavelength, adjust the light power density, and the light irradiation time.

[0021] Preferably, in step S4, record and evaluate the impact of LED light irradiation on the damaged endometrium:

[0022] Record the reactions and any adverse reactions of mice during the treatment process; through histological detection and fertility evaluation, analyze the morphological changes of the endometrium of mice before and after light irradiation and fertility indicators such as pregnancy rate and litter size.

[0023] Preferably, in step S4, the light source can select one or both of red light of 630 nm and infrared light of 850 nm for treating mice.

[0024] (III) Advantageous Effects

[0025] Compared with the prior art, the present invention provides an LED lighting device and a method for repairing damaged endometrium of mice, having the following advantageous effects:

[0026] The present invention uses histological methods to detect the endometrium of mice and evaluate the degree of endometrial damage in mice. According to the evaluation results, the wavelength, intensity, and irradiation time of LED lighting are set. The mice are irradiated with a multi-spectral LED light source, and the damaged area is treated with light according to the set parameters, having the advantages of less trauma, short recovery period, significantly reducing the risks of infection and adhesion, and promoting damage repair. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 is a schematic flow structure diagram of the present invention;

[0028] Figure 2 is a diagram of the LED lighting device for mouse experiments;

[0029] Figure 3 is a diagram of the effect of LED lighting with different wavelengths on the pregnancy rate of mice with damaged endometrium;

[0030] Figure 4 is a diagram of the thickening of the damaged endometrium of mice by 850nm LED phototherapy;

[0031] Figure 5 is a schematic structural diagram of the LED lighting device of the present invention. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0032] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0033] Example 1:

[0034] As shown in Figure 1 and 5 , an LED lighting device and a method for repairing damaged endometrium of mice include the following steps:

[0035] S1. Experimental grouping: Purchase several mice, check their health conditions, and randomly divide the mice into four groups, namely a normal group A, a damaged group B, an irradiation group C, and an irradiation group D;

[0036] S2. Establish a damage model: Establish a mouse model with damaged endometrium by perfusing absolute ethanol into the uterine cavity of mice.

[0037] S3. Lighting experiment: Place the normal group A and the damaged group B in the same breeding environment as the control group. Use a self-made LED lighting device, in which multi-spectral LED beads are installed on a flexible circuit board and arranged in a circular array on the inner wall of the device to ensure uniform light reception in the irradiated area of the mice.

[0038] Adjust the light density and wavelength of the LED lighting device, and perform light treatment on the damaged group of mice. Each light treatment lasts for 5 - 10 minutes, and continuous irradiation is carried out for 10 - 15 days, about 3 estrus cycles, to simulate the clinical treatment process. By detecting the pregnancy rate and the average litter size, evaluate the repair effect of light on the damaged endometrium, and further judge the fertility of the mice.

[0039] S4. Effect evaluation: Detect the repair effect of LED light on the damaged endometrium by evaluating the pregnancy rate and the average litter size of the mice. At the same time, use histological methods to evaluate the morphological and functional recovery of the endometrium.

[0040] Example 2:

[0041] As Figure 2 and Figure 5 shown, the LED lighting device includes a mounting shell, a fan assembly and a lighting assembly mounted on the mounting shell. The lighting assembly includes a multi-spectral LED light source, which is encapsulated on a flexible circuit board and mounted on the mounting shell. The flexible circuit board is connected to a controller;

[0042] The multi-spectral LED light source is used to generate light with a specific wavelength and intensity for performing a lighting experiment on mice.

[0043] Preferably, the multi-spectral LED light source includes a number of LED beads, and the LED beads are used to generate light with a specific wavelength and intensity;

[0044] A number of LED beads distributed inside the mounting shell are arranged in a circular pattern for irradiating mice;

[0045] The fan assembly includes a fan and a fan power supply. A fan is mounted on the mounting shell, and the fan is connected to a fan power supply; the controller is used to control the turning on or off of the light source, select the working wavelength, adjust the light power density, and the lighting time.

[0046] Example 3:

[0047] Design and application of LED light device for mouse experiments

[0048] Explore the repair effect of LED light on the damaged endometrium of mice through mouse experiments. An LED light therapy device dedicated to mouse experiments was designed. The mouse irradiation device adopted a circular irradiation scheme, arranging 9 * 37 LED beads in a circular array to achieve uniform light reception in the irradiated area of the mouse ( Figure 2 A). The light source was realized by encapsulating multi-spectral LED beads on a flexible circuit board ( Figure 2 B). The LED light therapy device included multiple components, including a light source, a power adapter, a fan power supply, a fan, and a controller. The main functions of the controller were to control the on / off of the light source, select the working wavelength, adjust the light power density, and the illumination time ( Figure 2 C). Subsequently, red light of 630 nm and infrared light of 850 nm were used for treatment to find the optimal light wavelength and energy density that could repair the damaged endometrium of mice.

[0049] Figure 2 LED lighting device for mouse experiments. (A) Schematic diagram of the circular irradiation device design to achieve uniform light reception in the irradiated area of the mouse. (B) Multi-spectral LED light source encapsulated on a flexible circuit board. (C) Device controller interface. (D) Status of mice irradiated with 630 nm and 850 nm LEDs.

[0050] Example 2, 850 nm LED light therapy can increase the pregnancy rate of mice with damaged endometrium

[0051] Table 1 Statistical table of the effects of LED light irradiation with different wavelengths on the pregnancy rate and litter size of mice with damaged endometrium. Normal mice were used as control mice

[0052] Grouping Normal - con Injury - con Injury + 630LED Injury + 850LED Number of female mice 6 6 6 6 Number of pregnancies 6(100.00％) 1(16.67％) 2(33.33％) 5(83.33％) Litter size 8.5±0.43a 2b 2.0±1.0b 2.0±0.45b

[0053] Figure 3 (A) Effects of LED light irradiation with different wavelengths on the pregnancy rate of mice with damaged endometrium. (B) Effects of LED light irradiation with different wavelengths on the litter size of mice with damaged endometrium. n = 6. Statistical analysis was performed using the t-test, and the data were expressed as mean ± SEM. *P < 0.05, **P < 0.01.

[0054] A mouse model of damaged endometrium was constructed by perfusing anhydrous ethanol into the uterine cavity of mice. Mice with damaged endometrium were irradiated with LED light of different wavelengths at a light intensity of 50 mW / cm2 for 5 minutes, and continuously irradiated for 15 days (about 3 estrus cycles, simulating 3 treatment courses of mouse experiments), and then fertility was detected. The results showed that the pregnancy rate of mice in the normal control group was 100% (normal-con), and the pregnancy rate of mice in the group with damaged endometrium without LED light irradiation (damage-con) was 16.67%, which was significantly decreased compared with the normal control group. The pregnancy rate of mice in the group with damaged endometrium irradiated with 630 nm LED light (damage + 630LED) was 33.33%. The pregnancy rate of mice in the group with damaged endometrium irradiated with 850 nm LED light (damage + 850LED) was 83.33%. The above results indicate that 850 nm LED light irradiation can significantly increase the pregnancy rate of mice with damaged endometrium (Table 1 and Figure 3 A). Further analyze the effect of LED light irradiation on the average litter size of mice with damaged endometrium. The average litter size of mice in the normal control group was 8.5 ± 0.43 per litter, the average litter size of mice in the group with damaged endometrium without LED light irradiation was 2 per litter, the average litter size of mice in the group with damaged endometrium irradiated with 630 nm LED light was 2.0 ± 1.0 per litter, and the average litter size of mice in the group with damaged endometrium irradiated with 850 nm LED light was 2.0 ± 0.45 per litter. LED light irradiation did not increase the average litter size of mice with damaged endometrium ( Figure 3 B).

[0055] Example 4:

[0056] 850 nm LED phototherapy can thicken the damaged endometrium of mice

[0057] Furthermore, the effect of light irradiation on the uterus of damaged mice was detected by histological methods. The results showed that the endometrium of mice was significantly thickened after 850 nm LED light irradiation compared with the damage control group ( Figure 4 ).

[0058] It should be noted that in this article, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the term "comprising", "including" or any other variant thereof is intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements not only includes those elements, but also includes other elements not expressly listed, or also includes elements inherent to such process, method, article or device.

[0059] Although embodiments of the present invention have been shown and described, it will be understood by those of ordinary skill in the art that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An LED lighting device and a method for repairing damaged endometrium in mice, characterized in that: The following steps are involved: S1. Experimental grouping: Several mice were purchased, their health status was checked, and the mice were randomly divided into four groups, namely normal group A, injury group B, irradiation group C and irradiation group D; S2. Construction of a mouse model of endometrial injury: A mouse model of endometrial injury was constructed by perfusing anhydrous ethanol into the mouse uterine cavity; S3. Lighting experiment: The mice were treated with light using a homemade LED lighting device. The normal group A and the injured group B were used as the control group and maintained in the same breeding environment. The irradiated group C and the irradiated group D were exposed to LED light of different wavelengths for 5-10 minutes each time for 10-15 days continuously, spanning three estrus cycles, simulating three treatment courses. S4. Effect evaluation: The pregnancy rate and average litter size of mice were evaluated to detect the repair effect of LED light on damaged endometrium. Histological methods were used to evaluate the morphological and functional recovery of the endometrium.

2. The LED lighting device and the method for repairing damaged endometrium of mice according to claim 1, characterized in that: The LED lighting device in step S3 comprises a mounting shell, and a fan assembly and a lighting assembly mounted on the mounting shell, wherein the lighting assembly comprises a multi-spectrum LED light source, wherein the multi-spectrum LED light source is packaged on a flexible circuit substrate and mounted on the mounting shell, and wherein the flexible circuit substrate is connected to a controller; The multi-spectrum LED light source is used to generate light of specific wavelength and intensity for performing light experiments on mice.

3. The LED lighting device according to claim 2, characterized in that: The multi-spectrum LED light source includes a plurality of LED lamp beads, and the LED lamp beads are used to generate light of a specific wavelength and intensity; The plurality of LED lamp beads distributed inside the mounting shell are distributed in a ring shape and are used to illuminate mice.

4. The LED lighting device according to claim 2, characterized in that: The fan assembly comprises a fan and a fan power supply. The fan is mounted on the mounting shell, and the fan is connected to the fan power supply.

5. The LED lighting device according to claim 2, characterized in that: The controller is used to control the on or off of the light source, select the working wavelength, and adjust the light power density and the illumination time.

6. The LED lighting device and the method for repairing damaged endometrium of mice according to claim 1, characterized in that: In step S4, the effect of LED illumination on the damaged endometrium is recorded and evaluated: The responses of the mice during the treatment and any adverse reactions were recorded; the morphological changes of the mouse endometrium before and after illumination as well as fertility indicators such as pregnancy rate and litter size were analyzed through histological examination and fertility assessment.

7. The LED lighting device and the method for repairing damaged endometrium of mice according to claim 1, characterized in that: In step S4, the light source can be selected to use one or both of 630nm red light and 850nm infrared light for treating mice.

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