Composition for degrading biodegradable mulching film as well as preparation method and use method of composition
By using a composition containing photocatalysts and microorganisms, the problem of biodegradable PBAT mulch degradation in arid and semi-arid areas is solved, and faster degradation and a healthier soil environment are achieved, reducing the impact on next season of crop cultivation.
Patent Information
- Application Number
- CN202510387396.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-31
- Publication Date
- 2025-06-20
AI Technical Summary
Biodegradable PBAT mulch degrades for a long time in arid and semi-arid areas and needs to be recycled, otherwise it will affect the next season of crop cultivation, and the degradation products may have potential impacts on the soil environment and plant growth.
A composition is adopted, including Product A and Product B, which consists of a dispersant, a defoamer, a photocatalyst, a vitamin E polyethylene glycol succinate and water, and Product B is composed of microorganisms, which accelerates the degradation of PBAT mulch by spraying Product A and Product B.
Through the use of the composition, the degradation process of PBAT mulch is significantly accelerated, the damage of degradation products to plants is reduced, and the soil environment is improved, avoiding the impact on crop planting in the next season.
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Abstract
Description
Technical Field
[0001] The present invention relates to a composition for degrading biodegradable mulch films and its preparation and use methods. Background Art
[0002] The northwest region is the main distribution area of arid and semi-arid regions in China, with scarce water resources and a fragile ecological environment. Therefore, film mulching is mostly used for crop planting. At present, biodegradable mulch films have the advantages of good thermal stability, good ductility, and good heat preservation, and have been widely used.
[0003] Biodegradable mulch films must meet the needs of the entire growth period of crops and not affect the planting of the next season's crops, which poses a challenge to biodegradable mulch films. The main impact on the next season's planting is that when planting the next season's crops, the degradation rate of biodegradable mulch films is low. If not recycled and turned into the ground for farming, it will affect water penetration and the growth of plant roots. In the long run, it will also lead to excessive microplastics in the soil, polluting the ecological environment.
[0004] The degradation of biodegradable mulch films in nature is affected by climate, degradation products, and the mulch films themselves, as follows: Gansu has an arid and semi-arid climate, with little annual precipitation and low air humidity, which leads to insufficient water supply for hydrolysis and inability to meet the water demand of microorganisms. Therefore, it affects the hydrolysis and biodegradation of biodegradable mulch films, that is, climate reasons prolong the degradation period of biodegradable mulch films.
[0005] Taking PBAT mulch films as an example, the degradation products of biodegradable mulch films are terephthalic acid (TPA) and adipic acid. Terephthalic acid (TPA) has broad-spectrum bactericidal properties, so it will also affect the degradation of biodegradable mulch films and prolong its degradation period.
[0006] In addition, the surface of the mulch film is smooth, and microorganisms are not easily attached to its surface, which also affects the degradation of biodegradable mulch films.
[0007] In summary, when biodegradable mulch films are applied in arid and semi-arid regions, their degradation period is significantly prolonged, and they need to be recycled during planting, otherwise it will affect the soil environment and crop growth.
[0008] Currently, there is no solution to the problem that the degradation time of degradable PBAT mulch films applied in arid and semi-arid regions is long and they need to be recycled, otherwise it will affect the planting of the next season's crops; nor is there a solution to the problem that the degradation of PBAT mulch films produces microplastics (MPs) and other intermediate products, which have potential impacts on the soil environment and plant growth and development. Summary of the Invention
[0009] The present invention provides a composition for degrading biodegradable mulch films, and its preparation and use methods. The technical problems to be solved are: 1) When biodegradable PBAT mulch films are applied in arid and semi-arid regions, due to climatic reasons, the degradation time is long and they need to be recycled, otherwise it will affect the planting of the next crop; 2) The microplastics (MPs) and other intermediate products generated during the degradation of PBAT mulch films have potential impacts on the soil environment and plant growth and development.
[0010] To solve the above technical problems, the present invention adopts the following technical solutions: A composition for degrading biodegradable mulch films, comprising Product A and Product B. Product A includes a dispersant, an antifoaming agent, a photocatalyst, vitamin E polyethylene glycol succinate, and water; Product B includes microorganisms. The microorganisms can degrade biodegradable mulch films. Product A and Product B are used in combination and are packaged separately.
[0011] The fineness of the photocatalyst is ≧1200 mesh. The photocatalyst is one or more of titanium dioxide, zinc oxide, and iron(III) oxide. The microorganisms are one or more of Paecilomyces lilacinus, Aspergillus terreus, and Bacillus spp.
[0012] Product B also includes vitamin E polyethylene glycol succinate.
[0013] A preparation method of the composition for degrading biodegradable mulch films, and Product A is prepared according to the following steps: 1) Add the dispersant and the antifoaming agent to water and mix evenly to obtain pretreated water. 2) Under stirring conditions, add the photocatalyst to the pretreated water and stir for 15 - 40 min to obtain a suspension. 3) Add vitamin E polyethylene glycol succinate to the suspension and mix evenly to obtain Product A. Product B is prepared according to the following steps: Package the microorganisms to obtain Product B; or Mix the microorganisms and vitamin E polyethylene glycol succinate evenly to obtain Product B. The combination of Product A and Product B is the composition for degrading biodegradable mulch films.
[0014] In step 1), the mass ratio of the dispersant, the antifoaming agent, and water is 0.1 - 0.5:0.1 - 0.5:99.0 - 99.8. In step 2), the mass ratio of the photocatalyst and the pretreated water is 1 - 20:80 - 99. In step 2), the stirring speed is 800 - 5000 r / min. The addition of polyethylene glycol succinate vitamin E described in step 3) is the addition of an aqueous solution of polyethylene glycol succinate vitamin E with a mass fraction of 1-23%; In step 3), the mass ratio of the aqueous solution of polyethylene glycol succinate vitamin E to the suspension is 0.01-20:80-99.99; In product B, the mass ratio of the microorganism to polyethylene glycol succinate vitamin E is 99-99.9:0.1-1.
[0015] For the method of using the composition for degrading biodegradable mulch films, after diluting product A with water, it is sprayed onto the surface of the PBAT mulch film by spraying; after the diluted product A is sprayed onto the surface of the PBAT mulch film, the exposure time to sunlight is greater than 20 days; The dilution multiple is 100-1000 times.
[0016] After diluting product B with water, it is sprayed onto the surface of the PBAT mulch film by spraying; The dilution multiple is 100-1000 times; Product B is used before turning the PBAT mulch film into the ground, and the time interval between the use of product B and product A is greater than 20 days.
[0017] The dosage of product A is 20-500 g / mu; the dosage of product B is 20-2000 g / mu.
[0018] The invention has the following beneficial technical effects: 1) By the cooperation of product A and product B in this application, it can not only achieve better degradation of the PBAT mulch film, but also reduce the harm of the degradation products to plants.
[0019] 2) In product A of this application, polyethylene glycol succinate vitamin E enables the photocatalyst to adhere to the film, thus solving the problems that the film surface is smooth, difficult to adhere, and the degradation effect is poor.
[0020] 3) Product B of this application also includes polyethylene glycol succinate vitamin E. Polyethylene glycol succinate vitamin E can not only enable microorganisms to better adhere to the film, but also encapsulate the microorganisms to form protection for the microorganisms, avoiding the poisoning of the microorganisms by the degradation products of the film degraded by the microorganisms in the early stage and affecting the degradation effect.
[0021] 4) In this application, the mass ratio of the microorganism to polyethylene glycol succinate vitamin E in product B is limited to 99-99.9:0.1-1. This is because when the addition amount of polyethylene glycol succinate vitamin E is large, it will cause the microorganisms to agglomerate and flocculate during use, thus affecting the use effect.
[0022] 5) Preparation method of product A of the present application: First, prepare pretreated water, then prepare a suspension, and then add vitamin E polyethylene glycol succinate to the suspension, so as to make the prepared product A have stable quality and good use effect.
[0023] 6) In terms of the usage method, product A is sprayed on the surface of the PBAT plastic film, and its exposure time to sunlight is greater than 20 days. This is because the active ingredient in product A is a photocatalyst, which will only degrade the film under light irradiation, and the longer the time, the better the effect. Limiting the exposure time to sunlight is to achieve a better degradation effect. At the same time, the degradation of the film by the photocatalyst destroys the smoothness of the film surface, making it easier for product B to adhere to the degraded film, which is beneficial to the degradation of the film by product B.
[0024] 7) The present application stipulates that product B is used before turning the PBAT plastic film into the ground, and the usage interval between product B and product A is greater than 20 days. Using it before turning it into the ground is to prevent product A from killing the microorganisms in product B and affecting the use effect. An interval of 20 days is conducive to giving full play to the effect of product A, providing a good environment for the adhesion of product B, and not affecting the use of product B. Specific Embodiments
[0025] In order to better understand the above technical solutions, the technical solutions of the present invention will be described in detail below in combination with preferred examples.
[0026] Example 1 A composition for degrading biodegradable plastic films, which is composed of product A and product B. Product A is composed of a dispersant, an antifoaming agent, a photocatalyst, vitamin E polyethylene glycol succinate and water; product B is a microorganism; Product A and product B are used in combination and are packaged separately.
[0027] The photocatalyst is zinc oxide with a fineness of 2000 meshes; The microorganism is Paecilomyces lilacinus.
[0028] Preparation method of the composition for degrading biodegradable plastic films: Product A is prepared according to the following steps: 1) Add a dispersant and an antifoaming agent to water, mix evenly to obtain pretreated water; 2) Under stirring conditions, add the photocatalyst to the pretreated water and stir for 20 min to obtain a suspension; 3) Add vitamin E polyethylene glycol succinate to the suspension and mix evenly to obtain product A; Product B is prepared according to the following steps: Package the microorganism to obtain product B; The combination of Product A and Product B is the composition for degrading biodegradable mulch films.
[0029] In step 1), the mass ratio of the dispersant, defoamer and water is 0.2:0.3:99.5; In step 2), the mass ratio of the photocatalyst and the pretreated water is 15:85; In step 2), the stirring speed is 2000 r / min; In step 3), adding vitamin E polyethylene glycol succinate means adding an aqueous solution of vitamin E polyethylene glycol succinate with a mass fraction of 15%; In step 3), the mass ratio of the aqueous solution of vitamin E polyethylene glycol succinate and the suspension is 12:88.
[0030] The usage method of the composition for degrading biodegradable mulch films is as follows: After diluting Product A with water, spray it on the surface of PBAT mulch films by means of spraying; The dilution multiple is 600 times.
[0031] After diluting Product B with water, spray it on the surface of PBAT mulch films by means of spraying; Product B is used before turning the PBAT mulch film into the ground, and the time interval between the use of Product B and Product A is 140 days.
[0032] The dilution multiples of both Product A and Product B are 600 times.
[0033] The dosage of Product A is 100 g / mu; the dosage of Product B is 200 g / mu.
[0034] The dispersant is dispersant 5040, purchased from Qingdao Enze Chemical Co., Ltd.; the defoamer is polyether defoamer, purchased from Yantai Hengxin Chemical Technology Co., Ltd.
[0035] Example 2 A composition for degrading biodegradable mulch films consists of Product A and Product B. Product A consists of a dispersant, a defoamer, a photocatalyst, vitamin E polyethylene glycol succinate and water; Product B consists of microorganisms and vitamin E polyethylene glycol succinate; Product A and Product B are used in combination and are packaged separately.
[0036] The photocatalyst is zinc oxide with a fineness of 2000 mesh; The preparation method of the composition for degrading biodegradable mulch films is as follows: Product A is prepared according to the following steps: 1) Add a dispersant and a defoamer to water, mix well to obtain pretreated water; 2) Under stirring conditions, add a photocatalyst to the pretreated water and stir for 20 min to obtain a suspension; 3) Add polyethylene glycol succinate of vitamin E to the suspension and mix evenly to obtain Product A; Product B is prepared according to the following steps: Mix the microorganism and polyethylene glycol succinate of vitamin E evenly to obtain Product B; The combination of Product A and Product B is the composition for degrading biodegradable mulch films.
[0037] In step 1), the mass ratio of the dispersant, defoamer and water is 0.2:0.3:99.5; In step 2), the mass ratio of the photocatalyst and the pretreated water is 15:85; In step 2), the stirring speed is 2000 r / min; In step 3), the addition of polyethylene glycol succinate of vitamin E means adding an aqueous solution of polyethylene glycol succinate of vitamin E with a mass fraction of 15%; In step 3), the mass ratio of the aqueous solution of polyethylene glycol succinate of vitamin E and the suspension is 12:88.
[0038] In Product B, the mass ratio of the microorganism and polyethylene glycol succinate of vitamin E is 99.5:0.5.
[0039] For the using method of the composition for degrading biodegradable mulch films, dilute Product A with water and then spray it on the surface of PBAT mulch film by means of spraying; The dilution multiple is 600 times.
[0040] Dilute Product B with water and then spray it on the surface of PBAT mulch film by means of spraying; Product B is used before turning the PBAT mulch film into the ground, and the time interval between the use of Product B and Product A is 140 days.
[0041] The dilution multiples of both Product A and Product B are 600 times.
[0042] The dosage of Product A is 100 g / mu; the dosage of Product B is 200 g / mu.
[0043] Example 3 A composition for degrading biodegradable mulch films is composed of Product A and Product B. Product A is composed of a dispersant, a defoamer, a photocatalyst, polyethylene glycol succinate of vitamin E and water; Product B is a microorganism; Product A and Product B are used in combination and are packaged separately.
[0044] The photocatalyst is titanium dioxide with a fineness of 2000 mesh; The microorganism is Paecilomyces lilacinus.
[0045] Preparation method of composition for degrading biodegradable mulch film. Product A is prepared according to the following steps: 1) Add a dispersant and an antifoaming agent to water, mix well to obtain pretreated water; 2) Under stirring conditions, add a photocatalyst to the pretreated water and stir for 30 min to obtain a suspension; 3) Add vitamin E polyethylene glycol succinate to the suspension and mix well to obtain Product A; Product B is prepared according to the following steps: Package the microorganism to obtain Product B; The combination of Product A and Product B is the composition for degrading biodegradable mulch film.
[0046] In step 1), the mass ratio of the dispersant, the antifoaming agent and water is 0.3:0.3:99.4; In step 2), the mass ratio of the photocatalyst and the pretreated water is 18:82; In step 2), the stirring speed is 3000 r / min; In step 3), the addition of vitamin E polyethylene glycol succinate is to add an aqueous solution of vitamin E polyethylene glycol succinate with a mass fraction of 15%; In step 3), the mass ratio of the aqueous solution of vitamin E polyethylene glycol succinate and the suspension is 15:85.
[0047] Usage method of the composition for degrading biodegradable mulch film. After diluting Product A with water, spray it on the surface of the PBAT mulch film by means of spraying; The dilution multiple is 600 times.
[0048] After diluting Product B with water, spray it on the surface of the PBAT mulch film by means of spraying; Product B is used before turning the PBAT mulch film into the ground, and the time interval between the use of Product B and Product A is 140 days.
[0049] The dilution multiples of both Product A and Product B are 600 times.
[0050] The dosage of Product A is 100 g / mu; the dosage of Product B is 200 g / mu.
[0051] The dispersant is dispersant 5040, purchased from Qingdao Enze Chemical Co., Ltd.; the antifoaming agent is a polyether antifoaming agent, purchased from Yantai Hengxin Chemical Technology Co., Ltd.
[0052] Example 4 A composition for degrading biodegradable mulch films, which is composed of Product A and Product B. Product A is composed of a dispersant, an antifoaming agent, a photocatalyst, vitamin E polyethylene glycol succinate, and water; Product B is composed of microorganisms and vitamin E polyethylene glycol succinate; Product A and Product B are used in combination and are packaged separately.
[0053] The photocatalyst is titanium dioxide with a fineness of 2000 mesh; A preparation method of the composition for degrading biodegradable mulch films. Product A is prepared according to the following steps: 1) Add the dispersant and the antifoaming agent to water and mix evenly to obtain pretreated water; 2) Under stirring conditions, add the photocatalyst to the pretreated water and stir for 30 min to obtain a suspension; 3) Add vitamin E polyethylene glycol succinate to the suspension and mix evenly to obtain Product A; Product B is prepared according to the following steps: Mix the microorganisms and vitamin E polyethylene glycol succinate evenly to obtain Product B; The combination of Product A and Product B is the composition for degrading biodegradable mulch films.
[0054] In step 1), the mass ratio of the dispersant, the antifoaming agent, and water is 0.3:0.3:99.4; In step 2), the mass ratio of the photocatalyst and the pretreated water is 18:82; In step 2), the stirring speed is 3000 r / min; In step 3), adding vitamin E polyethylene glycol succinate means adding an aqueous solution of vitamin E polyethylene glycol succinate with a mass fraction of 15%; In step 3), the mass ratio of the aqueous solution of vitamin E polyethylene glycol succinate and the suspension is 15:85.
[0055] In Product B, the mass ratio of the microorganisms and vitamin E polyethylene glycol succinate is 99.5:0.5.
[0056] A usage method of the composition for degrading biodegradable mulch films. After diluting Product A with water, spray it on the surface of PBAT mulch films by means of spraying; The dilution multiple is 600 times.
[0057] After diluting Product B with water, spray it on the surface of PBAT mulch films by means of spraying; Product B is used before turning the PBAT mulch films into the ground, and the time interval between the use of Product B and Product A is 140 days.
[0058] The dilution multiples of Product A and Product B are both 600 times.
[0059] The dosage of Product A is 100 g / mu; the dosage of Product B is 200 g / mu.
[0060] Example 5 A composition for degrading biodegradable mulch films is composed of Product A and Product B. Product A is composed of a dispersant, an antifoaming agent, a photocatalyst, vitamin E polyethylene glycol succinate, and water; Product B is composed of microorganisms and vitamin E polyethylene glycol succinate; Product A and Product B are used in combination and are packaged separately.
[0061] The photocatalyst is a composition of titanium dioxide and zinc oxide in a mass ratio of 1:1, and the fineness is 2000 mesh; The microorganisms are a composition of Paecilomyces lilacinus and Bacillus in a mass ratio of 1:2.
[0062] A preparation method of a composition for degrading biodegradable mulch films. Product A is prepared according to the following steps: 1) Add a dispersant and an antifoaming agent to water, mix evenly to obtain pretreated water; 2) Under stirring conditions, add the photocatalyst to the pretreated water and stir for 25 min to obtain a suspension; 3) Add vitamin E polyethylene glycol succinate to the suspension and mix evenly to obtain Product A; Product B is prepared according to the following steps: Mix the microorganisms and vitamin E polyethylene glycol succinate evenly to obtain Product B; The combination of Product A and Product B is the composition for degrading biodegradable mulch films.
[0063] In step 1), the mass ratio of the dispersant, the antifoaming agent, and water is 0.3:0.4:99.3; In step 2), the mass ratio of the photocatalyst and the pretreated water is 15:85; In step 2), the stirring speed is 2000 r / min; In step 3), the addition of vitamin E polyethylene glycol succinate is to add an aqueous solution of vitamin E polyethylene glycol succinate with a mass fraction of 18%; alcohol succinate; In step 3), the mass ratio of the aqueous solution of vitamin E polyethylene glycol succinate and the suspension is 12:88; In Product B, the mass ratio of the microorganisms and vitamin E polyethylene glycol succinate is 99.5:0.5.
[0064] Method for using the composition for degrading biodegradable mulch film. After diluting Product A with water, it is sprayed onto the surface of PBAT mulch film by means of spraying; the diluted Product A is sprayed onto the surface of PBAT mulch film, and the exposure time to sunlight is more than 20 days; The dilution multiple is 800 times.
[0065] Method for using the composition for degrading biodegradable mulch film. After diluting Product B with water, it is sprayed onto the surface of PBAT mulch film by means of spraying; The dilution multiple is 800 times; Product B is used before turning the PBAT mulch film into the ground, and the time interval between the use of Product B and Product A is more than 20 days.
[0066] The dosage of Product A is 100 g / mu; the dosage of Product B is 500 g / mu.
[0067] The dispersant is Dispersant 5040, purchased from Qingdao Enze Chemical Co., Ltd.; the defoamer is polyether defoamer, purchased from Yantai Hengxin Chemical Technology Co., Ltd.
[0068] The beneficial effects of the present invention are further illustrated below in conjunction with experimental data: Experiment 1 1.1 Test location: Gansu Smart Agriculture Engineering Technology Research Center.
[0069] 1.2 Experimental detection: The weight (g) of PBAT mulch film (including the initial weight, secondary weight and end weight, and calculate the difference between the two), and the experimental PBAT mulch film was purchased from Lanzhou Xinyinhuan Rubber and Plastic Products Co., Ltd.
[0070] 1.3 Test materials: Blank (treated with water), Comparative 1 (except omitting Step 1, that is, except for not adding defoamer and dispersant, others are the same as Example 1), Comparative 2 (except omitting Step 3, that is, except for not adding vitamin E polyethylene glycol succinate, others are the same as Example 1), Comparative 3 (except in Step 2, vitamin E polyethylene glycol succinate and photocatalyst are added to the pretreatment water together and mixed evenly to obtain the composition for degrading biodegradable mulch film, omitting Step 3), others are the same as Example 1), Example 1, Example 2, Example 3 and Example 4 prepared compositions for degrading biodegradable mulch film; PBAT mulch film.
[0071] 1.4 Experimental method: Before the experiment, weigh the PBAT mulch film to be treated, with a mass of about 30 g, which is recorded as the initial weight; after diluting each treatment 600 times with water, spray it onto the PBAT mulch film by spraying, and the mass ratio of the diluted each treatment to the PBAT mulch film is 1:100; each treatment refers to blank (treated with water), comparison 1 (except omitting step 1, that is, without adding defoamer and dispersant, and the others are the same as in Example 1), comparison 2 (except omitting step 3, that is, without adding vitamin E polyethylene glycol succinate, and the others are the same as in Example 1), comparison 3 (except in step 2, vitamin E polyethylene glycol succinate and photocatalyst are added to the pretreatment water and mixed evenly to obtain the composition for degrading biodegradable mulch film, and step 3 is omitted), and the others are the same as in Example 1), Example 1, Example 2, Example 3, and product A prepared in Example 4; conduct ultraviolet degradation, which simulates the ultraviolet degradation when the mulch film is laid on the ground surface. Specifically: place the treated PBAT mulch film under an ultraviolet lamp for 4 h, set the temperature to 45 °C, and then for 8 h under dark conditions at 30 °C, that is, 12 h is a cycle. Place a water tank during the experiment to ensure the humidity of the experimental conditions. The experimental period is 12 days, and weigh (do not wash); among them, Example 1 and Example 2 are each divided into two parts, one part is sprayed with product A, and the other part is sprayed with product B the day after spraying product A. The dilution factor of product B is 600 times. Among them, Example 1 and Example 2 that are sprayed with product A are recorded as Example 1 and Example 2, and Example 1 and Example 2 that are sprayed with product B the day after spraying product A are recorded as Comparative Example 1 and Comparative Example 2. Setting the temperature at 45 °C is to prevent killing Paecilomyces lilacinus due to excessive temperature.
[0072] Dark degradation: After diluting product B 600 times, spray it onto the mulch film after ultraviolet degradation and hang it on the sample rack of a constant temperature and humidity chamber. Under the conditions of no light, a temperature of 25 °C, and a humidity of 60% RH, keep it for 30 days. Rinse the treated mulch film with distilled water, dry it and weigh it, which is recorded as the final weight. Among them, the blank (water) is sprayed with water, and Comparative Example 1 and Comparative Example 2 are also sprayed with water. The amount of water sprayed is equal to the amount of diluted product B sprayed.
[0073] The dosage of product B is equal to the dosage of product A, and the mass ratio of the diluted product B to the PBAT after ultraviolet degradation is 1:100.
[0074] 1.5 Detection method: Weigh the mulch film with an electronic balance.
[0075] In this experiment, except for different experimental treatments, other operations are the same.
[0076] 2 Results and analysis Initial weight (g), secondary weight (g), final weight (g), and calculate the difference between the initial weight and the secondary weight and the difference between the initial weight and the final weight (considered as the degraded mass), which are recorded as difference a and difference b respectively, as shown in Table 1 Table 1
[0077] As can be seen from Table 1, the PBAT mulch film treated with blank (water treatment) degrades the least.
[0078] From the comparison of the data of Comparative Example 1 (except that step 1 is omitted, that is, no defoamer and dispersant are added, and the others are the same as those in Example 1), Comparative Example 2 (except that step 3 is omitted, that is, no vitamin E polyethylene glycol succinate is added, and the others are the same as those in Example 1) and Example 1, it can be seen that any change in the conditions of the defoamer, dispersant and vitamin E polyethylene glycol succinate will affect the use effect of the present application and they are inseparable.
[0079] From the comparison of the data of Comparative Example 3 (except that in step 2, vitamin E polyethylene glycol succinate and photocatalyst are added to the pretreated water and mixed evenly to obtain the composition for degrading biodegradable mulch film, and step 3 is omitted) and Example 1, it can be seen that changing the steps in the preparation method will also affect the use effect.
[0080] From the comparison of the data of Example 1 and Example 1 and the comparison of the data of Example 2 and Example 2, it can be seen that the difference between the initial weight and the secondary weight is very similar. The reason may be that product A is the same, while the difference between the initial weight and the final weight is enlarged. This may be because the bactericidal effect of the photocatalyst in product A causes the death of Paecilomyces lilacinus in Example 1 and Example 2, resulting in a large difference in the final weight. That is, the use method will also affect the use effect of the present application. This effect is obviously not as large as that of Comparative Example 1, Comparative Example 2 and Comparative Example 3. At the same time, it also shows that Paecilomyces lilacinus can further degrade biodegradable mulch film.
[0081] From the comparison between Example 1 and Example 2 and the comparison between Example 3 and Example 4, it can be seen that adding vitamin E polyethylene glycol succinate to Paecilomyces lilacinus of product B can further improve its degradation effect. The reason may be that vitamin E polyethylene glycol succinate makes Paecilomyces lilacinus more easily attached to the mulch film and forms a protective film on the surface of Paecilomyces lilacinus to prevent the degradation products generated by the PBAT degradable mulch film from poisoning Paecilomyces lilacinus.
[0082] According to the test effect of Experiment 1, Experiment 2 is preferentially carried out.
[0083] Experiment 2 1.1 Test location: Gansu Smart Agriculture Engineering Technology Research Center.
[0084] 1.2 Experimental detection: Root activity (mg / g.h).
[0085] 1.3 Test materials: Blank (no PBAT film added to the potting soil, only soil), Control 4 (the PBAT film added to the soil is the one degraded by ultraviolet light, and the ultraviolet degradation uses Product A prepared in Example 1), and the PBAT mulch films treated in Example 1, Example 2, Example 3, and Example 4. The PBAT film degraded by ultraviolet light used in Control 4 is the product obtained by the ultraviolet degradation method in Experiment 1. The product B is diluted 600 times with water and sprayed on the PBAT film degraded by ultraviolet light. The mass ratio of the diluted product B to the PBAT film degraded by ultraviolet light is 1:100. After drying, it is added to the soil. The mass ratio of the PBAT film treated by ultraviolet degradation to the soil is 2:98. Then it is ground and sieved through a 1-mm sieve, and filled into flower pots, 1.5 kg per pot. The well-seedling lettuce is transplanted into the pots, watered, and the soil moisture content is controlled at about 65%. Three plants are planted in each pot. The selected lettuce is of the same size visible to the naked eye. The soil moisture content is controlled by adding water. After growing for 45 days, it is harvested. After harvesting, the root activity is measured. The planting experiment is carried out in a greenhouse. The experiment is repeated 4 times in parallel.
[0086] 1.4 Experimental method: Take the soil that has not been used with agricultural film for 4 years, pick out stones, branches, and tree roots, and dry it in an oven at 80 °C for 30 min, sterilize and kill insects, and reserve for use; Take the PBAT film obtained by the ultraviolet degradation method in Experiment 1. Dilute product B 600 times with water and spray it on the PBAT film degraded by ultraviolet light. The mass ratio of the diluted product B to the PBAT film degraded by ultraviolet light is 1:100. After drying, it is added to the soil. The mass ratio of the PBAT film treated by ultraviolet degradation to the soil is 2:98. Then it is ground and sieved through a 1-mm sieve, and filled into flower pots, 1.5 kg per pot. The well-seedling lettuce is transplanted into the pots, watered, and the soil moisture content is controlled at about 65%. Three plants are planted in each pot. The selected lettuce is of the same size visible to the naked eye. The soil moisture content is controlled by adding water. After growing for 45 days, it is harvested. After harvesting, the root activity is measured. The planting experiment is carried out in a greenhouse. The experiment is repeated 4 times in parallel. For the blank (no PBAT film added to the potting soil, only soil), water is sprayed throughout the process. The amount of water sprayed during ultraviolet degradation is equal to the amount of the diluted product A, and the amount used when spraying on the ultraviolet-degraded film is equal to the amount of the diluted product B. 1.4 Experimental method: Take the soil that has not been used with agricultural film for 4 years, pick out stones, branches, and tree roots, and dry it in an oven at 80 °C for 30 min, sterilize and kill insects, and reserve for use; Take the PBAT film obtained by the ultraviolet degradation method in Experiment 1. Dilute product B 600 times with water and spray it on the PBAT film degraded by ultraviolet light. The mass ratio of the diluted product B to the PBAT film degraded by ultraviolet light is 1:100. After drying, it is added to the soil. The mass ratio of the PBAT film treated by ultraviolet degradation to the soil is 2:98. Then it is ground and sieved through a 1-mm sieve, and filled into flower pots, 1.5 kg per pot. The well-seedling lettuce is transplanted into the pots, watered, and the soil moisture content is controlled at about 65%. Three plants are planted in each pot. The selected lettuce is of the same size visible to the naked eye. The soil moisture content is controlled by adding water. After growing for 45 days, it is harvested. After harvesting, the root activity is measured. The planting experiment is carried out in a greenhouse. The experiment is repeated 4 times in parallel. For the blank (no PBAT film added to the potting soil, only soil), water is sprayed throughout the process. The amount of water sprayed during ultraviolet degradation is equal to the amount of the diluted product A, and the amount used when spraying on the ultraviolet-degraded film is equal to the amount of the diluted product B.
[0087] 1.5 Detection method: The root activity is measured according to the method of triphenyltetrazolium chloride (TTC).
[0088] Except for the different experimental treatments, other operations in this experiment are the same.
[0089] 2 Results and analysis Root activity (mg / g·h), recorded as activity (mg / g·h) in the table, see Table 2 Table 2
[0090] From the comparison of the data of the blank and Control 4, it can be seen that the degraded mulch film has an impact on the growth of the soil and plants, affecting the root activity of the plants.
[0091] From the data comparison of the blank, Comparative Example 4, Example 1, Example 2, Example 3, and Example 4, it can be seen that the root activity of Example 1, Example 2, Example 3, and Example 4 is higher than that of Comparative Example 4 and lower than that of the blank. It may be that when Paecilomyces lilacinus degrades the PBAT film, it also secretes substances that promote plant root growth, or it may be that Paecilomyces lilacinus degrades some microplastics, thereby offsetting part of the impact of degrading the mulch film. In particular, the root activity of Example 2 and Example 4 is basically equivalent to that of the blank, that is, adding vitamin E polyethylene glycol succinate to the microorganism can further offset the impact of degrading the mulch film.
[0092] Experiment Three 1.1 Test site: Dingxi City, Gansu Province. Potato test variety: Wotu No. 5.
[0093] 1.2 Experimental detection: Plant height (cm), number of single potatoes (pcs), and average yield (kg / mu).
[0094] 1.3 Test materials: Comparative Example 5 (only using Product A prepared in Example 1 to treat the PBAT film), Example 1, Example 2, PBAT mulch films treated in Example 3 and Example 4. Since Gansu has an arid and semi-arid climate and all plantings use film mulching, a blank test cannot be carried out in the field experiment.
[0095] 1.4 Experimental method: Potatoes were planted in the previous season (planted at the end of April 2023). The mulch film used was a PBAT mulch film purchased from Lanzhou Xinyinhuan Rubber and Plastic Products Co., Ltd. and applied on September 25, 2023. Product A prepared in Comparative Example 5 (Example 1), Example 1, Example 2, Example 3, and Example 4 were used to treat the laid PBAT mulch films in the set treatment plots respectively. The dosage was 5 kg / mu, and the usage method was to dilute Product A 400 times with water and then apply it by spraying. The harvest was on October 3, 2023. The area corresponding to each treatment was one mu. The land was plowed on April 10, 2024. Before plowing, Product B was diluted 500 times and applied by spraying. The dosage of Product B was 5 kg / mu. Among them, Comparative Example 5 (Product A prepared in Example 1) was sprayed with water of the same mass as the diluted Product A. The PBAT mulch film was turned into the ground. The PBAT mulch film was not recycled before that. Potatoes were replanted on April 25, 2024, still using film mulching. The mulch film was a PBAT mulch film purchased from Lanzhou Xinyinhuan Rubber and Plastic Products Co., Ltd., and sub-surface drip irrigation was used. The harvest time was October 2, 2024.
[0096] Except for different experimental treatments, other operations in this experiment were the same.
[0097] 2 Results and Analysis Plant height (cm), number of single sweet potatoes (pcs), and yield (kg / mu) were all averaged, as shown in Table 3 Table 3
[0098] From the data comparison of Example 1 and Example 1 in Table 1 and that of Comparative Example 5 and Example 1 in Table 3, it can be seen that adding Paecilomyces lilacinus can not only further degrade the biodegradable mulch film, but also offset the impact of the degradation products of the biodegradable mulch film on plants, thereby increasing crop yield.
[0099] From the comparison of Example 1 and Example 2, as well as Example 3 and Example 4, it can be seen that adding vitamin E polyethylene glycol succinate to Product B can further increase crop yield. The possible reason is that vitamin E polyethylene glycol succinate makes Paecilomyces lilacinus more likely to adhere to the biodegradable mulch film for colonization. At the same time, vitamin E polyethylene glycol succinate forms micelles to protect Paecilomyces lilacinus, enabling it to form a population effect after colonization and reducing the impact of the degradation products of the biodegradable mulch film on it.
Claims
1. A composition for degrading biodegradable mulch film, characterized in that: The invention comprises product A and product B, wherein product A comprises a dispersant, a defoaming agent, a photocatalyst, vitamin E polyethylene glycol succinate and water; and product B comprises a microorganism; The microorganisms are capable of degrading biodegradable mulch films; The product A and product B are used together and are packaged separately.
2. The composition for degrading biodegradable mulch film according to claim 1, characterized in that: The photocatalyst has a fineness of ≧1200 mesh; The photocatalyst is one or more of titanium dioxide, zinc oxide and ferric oxide; The microorganism is one or more of Paecilomyces lilacinus, Aspergillus terreus and Bacillus.
3. The composition for degrading biodegradable mulch film according to claim 1, characterized in that: Product B also includes vitamin E polyethylene glycol succinate.
4. The method for preparing a composition for degrading biodegradable mulch film according to any one of claims 1 to 3, characterized in that: Product A follows the steps below: 1) Add dispersant and defoamer to water and mix well to obtain pretreated water; 2) Add photocatalyst to the pretreated water under stirring conditions and stir for 15 to 40 minutes to obtain a suspension; 3) Adding vitamin E polyethylene glycol succinate to the suspension and mixing well to obtain product A; Product B follows the steps below: Packaging the microorganisms to obtain product B; or The microorganism and vitamin E polyethylene glycol succinate are mixed to obtain product B; The combination of product A and product B is a composition for degrading biodegradable mulch.
5. The method for preparing a composition for degrading biodegradable mulch film according to claim 3, characterized in that: In step 1), the mass ratio of dispersant, defoamer and water is 0.1-0.5:0.1-0.5:99.0-99.8; In step 2), the mass ratio of the photocatalyst to the pretreated water is 1-20:80-99; The stirring speed in step 2) is 800-5000 r / min; The step 3) wherein the vitamin E polyethylene glycol succinate is added is 1 to 23% by mass of vitamin E polyethylene glycol succinate. Aqueous solution of alcohol succinate; In step 3), the mass ratio of the aqueous solution of vitamin E polyethylene glycol succinate to the suspension is 0.01-20:80-99.99; The mass ratio of microorganisms to vitamin E polyethylene glycol succinate in product B is 99-99.9:0.1-1.
6. The method for using the composition for degrading biodegradable mulch film according to any one of claims 1 to 5, characterized in that: Product A is diluted with water and then sprayed on the surface of the PBAT mulch film by spraying; the diluted product A is sprayed on the surface of the PBAT mulch film and is exposed to sunlight for more than 20 days; The dilution multiple is 100 to 1000 times.
7. The method for using the composition for degrading biodegradable mulch film according to claim 5, characterized in that: Dilute product B with water and spray it on the surface of PBAT mulch film; The dilution multiple is 100 to 1000 times; Product B is used before burying the PBAT mulch film underground, and the interval between the use of Product B and Product A is greater than 20 days.
8. The method for using the composition for degrading biodegradable mulch film according to claim 6, characterized in that: The dosage of product A is 20-500g / mu; the dosage of product B is 20-2000g / mu.